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Bull Exp Biol Med
; 173(2): 261-264, 2022 Jun.
Article
En
| MEDLINE
| ID: mdl-35737160
We performed a comparative quantitative analysis of LINE-1 mRNA levels in extracellular total plasma RNA in patients with colon cancer and practically healthy donors. Quantitative multiplex PCR with reverse transcription was used to assess the level of LINE-1 and 18S rRNA mRNA in extracellular total plasma RNA. The median of LINE-1 mRNA values in colon cancer patients (4.95) was significantly higher than in healthy donors (2.3) (p=0.037). It was shown for the first time that the level of LINE-1 mRNA in total RNA from blood plasma can be determined in the format of a liquid biopsy and serve as a new potential non-invasive marker of colon cancer.
Cell-Free Nucleic Acids , Colonic Neoplasms , Colorectal Neoplasms , RNA-Binding Proteins , Biomarkers, Tumor/blood , Biomarkers, Tumor/genetics , Cell-Free Nucleic Acids/blood , Cell-Free Nucleic Acids/genetics , Colonic Neoplasms/blood , Colonic Neoplasms/genetics , Colorectal Neoplasms/blood , Colorectal Neoplasms/genetics , Colorectal Neoplasms/pathology , Humans , RNA, Messenger/blood , RNA, Messenger/genetics , RNA-Binding Proteins/genetics , Reverse Transcriptase Polymerase Chain Reaction
