ABSTRACT
This is the second study of a two-prong investigation conducted to learn two different aspects of vasovasostomy in dog vas deferens by using the carbon dioxide milliwatt laser. This investigation involves the evaluation of patency and analysis of sperm. Six dogs underwent vasectomy, followed by vasovasostomies performed between 4 and 12 weeks later, utilizing two approximating sutures and welding with the carbon dioxide milliwatt laser. Metal clips were used both for x-ray analysis and localization of the vasovasostomy site. All dogs had pre-vasectomy semen analysis. Following vasectomy each dog underwent ejaculation until azoospermia was obtained. Vasovasostomy was performed and semen was examined. Prior to harvesting, vasography was also obtained. All ejaculates had active sperm noted and 12 of 12 vasograms (100%) revealed patency. The dogs were sacrificed at 4 weeks, 6 weeks, and 8 weeks post-vasovasostomy. The vasovasostomy specimens were studied with electron microscopy and by histological evaluation. The vasovasostomy was completed in under 90 minutes by using this laser-assisted technique. This procedure offers to the urologist a more simplified and reproducible operation that can be performed easily and with a potential decrease in hospital costs.