ABSTRACT
New reactive analogs of substrates for DNA repair enzyme E. coli Fpg protein containing the residues of 8-oxoguanine and photoactivatable phenyl(trifluoromethyl)diazirine groups were synthesized. Their substrate properties were investigated. Using photocross-linking technique, we established the presence of contacts of two nucleosides located near the oxoG with amino acids from the Fpg protein. The cross-linking efficiency achieved 10%.
Subject(s)
DNA-Formamidopyrimidine Glycosylase/metabolism , DNA/metabolism , Diazomethane , Escherichia coli/enzymology , Oligodeoxyribonucleotides/metabolism , Base Sequence , Cross-Linking Reagents , DNA/chemistry , Oligodeoxyribonucleotides/chemistryABSTRACT
Photoreactive cross-linking reagents that simultaneously contain a trifluoromethyldiazirine and an o-nitrobenzyl groups were synthesized for the first time. Photochemical properties of the reagents were studied, and the possibility of separate activation of the diazirine group and o-nitrobenzyl linker was shown.