ABSTRACT
African swine fever (ASF) is a fatal disease that threatens the health status of the swine population and thus can impact the economic outcome of the global pig industry. Monitoring the ASF virus (ASFV) is of utmost concern to prevent and control its distribution. This study aims to identify a suitable sampling strategy for ASFV detection in living and deceased pigs under field conditions. A range of samples, comprising tissues obtained from deceased pigs, as well as serum and tonsil swab samples from live pigs, were gathered and subjected to detection using the qPCR method. The findings revealed that the mandibular lymph nodes demonstrated the highest viral loads among superficial tissues, thereby indicating their potential suitability for detecting ASFV in deceased pigs. Additionally, the correlations between virus loads in various tissues have demonstrated that tonsil swab samples are a viable specimen for monitoring live pigs, given the strong associations observed with other tissues. These findings indicated two dependable sample types for the detection of ASFV: mandibular lymph nodes for deceased pigs and tonsil swabs for live pigs, which supply some references for the development of efficacious preventive measures against ASFV.
ABSTRACT
Adsorption methods offer efficient recovery of phosphorus from water bodies. Modification adsorption materials combining lanthanum (La) and zirconium (Zr) dual-metal immobilized via co-precipitation method have been widely applied in the adsorption and recovery of phosphate. Meanwhile, sodium carbonate (Na2CO3) is gradually replacing sodium hydroxide (NaOH) as the mainstream co-precipitant for immobilizing metals into supporting matrices due to its excellent performance and environmental friendliness. However, the adsorption mechanisms of materials synthesized with different co-precipitants and the synergistic effects between dual-metal components are not well understood, which is not conducive to the further optimization of dual-metal adsorption materials. In this study, anion exchange resin was utilized as the supporting matrices, and La&Zr dual-metal-modified materials, La&Zr-CO32- and La&Zr-OH-, were prepared using Na2CO3 and NaOH as co-precipitants, respectively. The results indicate that La&Zr-CO32- exhibits superior performance in phosphate adsorption and recovery, with adsorption capacity and recovery efficiency reaching 36.28 mg/g and 82.59%, respectively. Additionally, this material demonstrates strong stability in reuse, phosphate selectivity, and a wide pH applicability range. La&Zr-CO32- achieves phosphate adsorption through surface electrostatic affinity, ligand exchange, and intraspherical complexation, whereas La&Zr-OH- primarily relies on electrostatic adsorption on the surface and interior of the material. Synergistic effects between La and Zr result in enhanced adsorption performance of the dual-metal material compared to individual metals. Specifically, phosphate adsorption is predominantly governed by La, while the presence of Zr further enhances ligand exchange between lattice oxygen and metals. Simultaneously, Zr doping enhances the phosphate recovery capacity and reusability of the materials. Continuous flow adsorption results from actual water bodies demonstrate that La&Zr-CO32- is more suitable for the removal and recovery of phosphate in water treatment engineering. This study provides a theoretical basis and technical support for the adsorption and recovery of phosphate using dual-metal-modified materials.
Subject(s)
Carbonates , Lanthanum , Phosphates , Water Pollutants, Chemical , Zirconium , Adsorption , Zirconium/chemistry , Lanthanum/chemistry , Carbonates/chemistry , Phosphates/chemistry , Water Pollutants, Chemical/chemistry , Water Purification/methods , Chemical PrecipitationABSTRACT
African swine fever (ASF) is a severe, hemorrhagic, and highly contagious disease caused by the African swine fever virus (ASFV) in both domestic pigs and wild boars. In China, ASFV has been present for over six years, with three genotypes of strains prevalent in field conditions: genotype I, genotype II, and genotype I/II recombinant strains. In order to differentiate among these three ASFV genotypes, a duplex fluorescent quantitative PCR method was established using specific probes and primers designed based on viral genes MGF_110-1L and O61R from ASFV strains reported in the GenBank database. Following optimization of reaction conditions, a duplex fluorescent quantitative PCR method was successfully developed. This method demonstrated no cross-reactivity with porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), porcine reproductive and respiratory syndrome virus (PRRSV), classic swine fever virus (CSFV), porcine pseudorabies virus (PRV), porcine circovirus 2 (PCV2), porcine circovirus 3 (PCV3), highlighting its specificity. Sensitivity analysis revealed that the limits of detection (LODs) of this method were 2.95 × 10-1 copies/µL for the MGF_110-1L gene and 2.95 × 100 copies/µL for the O61R gene. The inter- and intra-group coefficients of variation were both <1%, indicating high reproducibility. In summary, the establishment of this duplex fluorescent quantitative PCR method not only addresses the identification of the ASFV recombinant strains but also allows for simultaneous identification of the three epidemic genotype strains.
ABSTRACT
Effective evaluation of water quality and accurate quantification of pollution sources are essential for the sustainable use of water resources. Although water quality index (WQI) and positive matrix factorization (PMF) models have been proven to be applicable for surface water quality assessments and pollution source apportionments, these models still have potential for further development in today's data-driven, rapidly evolving technological era. This study coupled a machine learning technique, the random forest model, with WQI and PMF models to enhance their ability to analyze water pollution issues. Monitoring data of 12 water quality indicators from six sites along the Minjiang River from 2015 to 2020 were used to build a WQI model for determining the spatiotemporal water quality characteristics. Then, coupled with the random forest model, the importance of 12 indicators relative to the WQI was assessed. The total phosphorus (TP), total nitrogen (TN), chemical oxygen demand (CODCr), dissolved oxygen (DO), and five-day biochemical oxygen demand (BOD5) were identified as the top five significant parameters influencing water quality in the region. The improved WQI model constructed based on key parameters enabled high-precision (R2 = 0.9696) water quality prediction. Furthermore, the feature importance of the indicators was used as weights to adjust the results of the PMF model, allowing for a more reasonable pollutant source apportionment and revealing potential driving factors of variations in water quality. The final contributions of pollution sources in descending order were agricultural activities (30.26%), domestic sewage (29.07%), industrial wastewater (26.25%), seasonal factors (6.45%), soil erosion (6.19%), and unidentified sources (1.78%). This study provides a new perspective for a comprehensive understanding of the water pollution characteristics of rivers, and offers valuable references for the development of targeted strategies for water quality improvement.
Subject(s)
Water Pollutants, Chemical , Water Quality , Environmental Monitoring/methods , Random Forest , Water Pollutants, Chemical/analysis , Water Pollution/analysis , Rivers , ChinaABSTRACT
African swine fever (ASF), caused by the African swine fever virus (ASFV), is a highly contagious and notifiable animal disease in domestic pigs and wild boars, as designated by the World Organization for Animal Health (WOAH). The effective diagnosis of ASF holds great importance in promptly controlling its spread due to its increasing prevalence and the continuous emergence of variant strains. This paper offers a comprehensive review of the most common and up-to-date methods established for various genes/proteins associated with ASFV. The discussed methods primarily focus on the detection of viral genomes or particles, as well as the detection of ASFV associated antibodies. It is anticipated that this paper will serve as a reference for choosing appropriate diagnostic methods in diverse application scenarios, while also provide direction for the development of innovative technologies in the future.
ABSTRACT
Introduction: Pseudorabies virus (PRV) is a linear DNA virus with a double-stranded structure, capable of infecting a diverse array of animal species, including humans. This study sought to ascertain the seroprevalence of Pseudorabies Virus (PRV) in China by conducting a comprehensive collection of blood samples from 16 provinces over the course of 2022. Methods: The presence of PRV gE antibodies was detected through the utilization of an enzyme-linked immunosorbent assay (ELISA) technique. Logistic regression analysis was conducted to identify potential related factors associated with the serologic status of PRV gE at the animal level. Additionally, the SaTScan 10.1 software was used to analyze the spatial and temporal clusters of PRV gE seroprevalence. Results: A comprehensive collection of 161,880 samples was conducted, encompassing 556 swine farms throughout the country. The analysis revealed that the seroprevalence of PRV gE antibodies was 12.36% (95% confidence interval [CI], 12.20% to 12.52%) at the individual animal level. However, at the swine farm level, the seroprevalence was considerably higher, reaching 46.22% (95% CI, 42.08% to 50.37%). Related factors for PRV infection at the farm level included the geographic distribution of farms and seasonal variables. Moreover, five distinct high seroprevalence clusters of PRV gE were identified across China, with the peak prevalence observed during the months of April through June 2022. Conclusion: Our findings serve as a valuable addition to existing research on the seroprevalence, related factors, and temporal clustering of PRV gE in China. Furthermore, our study provides a reference point for the development of effective strategies for the prevention and control of pseudorabies and wild virus outbreaks.
ABSTRACT
The transmission of viral aerosols poses a vulnerable aspect in the biosecurity measures aimed at preventing and controlling swine virus in pig production. Consequently, comprehending and mitigating the spread of aerosols holds paramount significance for the overall well-being of pig populations. This paper offers a comprehensive review of transmission characteristics, influential factors and preventive strategies of common swine viral aerosols. Firstly, certain viruses such as foot-and-mouth disease virus (FMDV), porcine reproductive and respiratory syndrome virus (PRRSV), influenza A viruses (IAV), porcine epidemic diarrhea virus (PEDV) and pseudorabies virus (PRV) have the potential to be transmitted over long distances (exceeding 150 m) through aerosols, thereby posing a substantial risk primarily to inter-farm transmission. Additionally, other viruses like classical swine fever virus (CSFV) and African swine fever virus (ASFV) can be transmitted over short distances (ranging from 0 to 150 m) through aerosols, posing a threat primarily to intra-farm transmission. Secondly, various significant factors, including aerosol particle sizes, viral strains, the host sensitivity to viruses, weather conditions, geographical conditions, as well as environmental conditions, exert a considerable influence on the transmission of viral aerosols. Researches on these factors serve as a foundation for the development of strategies to combat viral aerosol transmission in pig farms. Finally, we propose several preventive and control strategies that can be implemented in pig farms, primarily encompassing the implementation of early warning models, viral aerosol detection, and air pretreatment. This comprehensive review aims to provide a valuable reference for the formulation of efficient measures targeted at mitigating the transmission of viral aerosols among swine populations.
ABSTRACT
BACKGROUND: Batch production, a widely implemented production model in large-scale pig farms, was characterized by its long-term duration, cost-effectiveness, and efficiency. Nevertheless, the recent occurrence of African swine fever (ASF) outbreaks in China has necessitated the implementation of discreet mating operations within this model, leading to disruptions in production cycles and substantial indirect losses. CASE PRESENTATION: This study implemented a novel operational procedure, which involved the division of risk areas for zone management and allowed mating operations, in 12 farms experiencing ASF outbreaks. Another 12 farms were used as a control group, employing the old procedure. Subsequently, the prognoses of both the old and new procedures were calculated and analyzed. The findings indicate that the new method resulted in an enhanced retention rate and reduced non-productive days (NPD), without impacting the positive detection rate and disposal time. Consequently, this approach significantly mitigated economic losses (p < 0.05). CONCLUSION: The efficacy of the novel procedure in mitigating the indirect economic losses stemming from ASF outbreaks, through the reduction of NPD while maintaining retention rates and disposition days, has been substantiated. This methodology has demonstrated feasibility in extensive pig farming operations and exhibits promise for broader application.
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Introduction: Porcine circovirus type 2 (PCV2) is the primary etiological agent of porcine circovirus diseases (PCVD), which are widespread in most pig herds, causing huge economic losses in the global pig industry. Therefore, it is critical to assess the infection characteristics of PCV2 in different swine herds to develop effective strategies against PCVD. Methods: In this study, routine diagnostic and monitoring protocols were used to collect 12,714 samples from intensive farms in China, and PCV2 was tested for by qPCR to determine positivity rates and viral loads in samples from different herds and materials. Results: PCV2 was found to be prevalent throughout China, and fattening farms had higher positivity rates than breeding farms. The PCV2 positivity rates in breeding farms in Southern China were higher than those in Northern China. Growing-finishing pigs demonstrated the highest positivity rate in the tested samples, while pre-weaning piglets and adult sows had the lowest. Meanwhile, samples with viral loads exceeding 106 copies/mL in growing-finishing pigs had 27.2% positivity, compared to 1.9% and 3.3% in sows and piglets, respectively. The results of the viral loads in the serum samples followed a similar trend. Discussion: The findings reveal that PCV2 circulates in different herds from intensive farms, with positivity increasing from pre-weaning to growing-finishing herds. It is urgent to develop effective strategies to reduce PCV2 positivity in growing-finishing herds and prevent viral circulation among pigs.
ABSTRACT
African swine fever (ASF) is a devastating and economically significant infectious disease that has caused enormous losses in the commercial pig sector in China since 2018. The primary transmission routes of the African swine fever virus (ASFV), the causative agent of ASF, are direct pig-to-pig contact or indirect contact with virus-contaminated objects. While aerosol transmission of ASFV has been previously reported under experimental conditions, no reports have described it under field conditions. In this case study, aerosol-associated samples were collected over a monitoring period of 24 days in an ASFV-positive farm. A complete and clear chain of ASFV transmission through aerosols was observed: pigs in Room A on Day 0-aerosol in Room A on Day 6-dust of air outlets in Room A on Day 9-outdoor aerosols on Day 9-dust of air inlets in Room B on Day 15-aerosols/pigs in Room B on Day 21. Furthermore, a fluorescent powder experiment confirmed the transmission of dust from Room A to Room B. This study represents the first report providing evidence of aerosol transmission of ASFV under field conditions. Further research is needed to study the laws of aerosol transmission in ASFV and develop effective strategies such as air filtration or disinfection to create a low-risk environment with fresh air for pig herds.
ABSTRACT
To effectively control pollution and improve water quality, it is essential to accurately analyze the potential pollution sources in rivers. The study proposes a hypothesis that land use can influence the identification and apportionment of pollution sources and tested it in two areas with different types of water pollution and land use. The redundancy analysis (RDA) results showed that the response mechanisms of water quality to land use differed among regions. In both regions, the results indicated that the water quality response relationship to land use provided important objective evidence for pollution source identification, and the RDA tool optimized the procedure of source analysis for receptor models. Positive matrix decomposition (PMF) and absolute principal component score-multiple linear regression (APCS-MLR) receptor models identified five and four pollution sources along with their corresponding characteristic parameters. PMF attributed agricultural nonpoint sources (23.8%) and domestic wastewater (32.7%) as the major sources in regions 1 and 2, respectively, while APCS-MLR identified mixed sources in both regions. In terms of model performance parameters, PMF demonstrated better-fit coefficients (R2) than APCS-MLR and had a lower error rate and proportion of unidentified sources. The results show that considering the effect of land use in the source analysis can overcome the subjectivity of the receptor model and improve the accuracy of pollution source identification and apportionment. The results of the study can help managers clarify the priorities of pollution prevention and control, and provide a new methodology for water environment management in similar watersheds.
Subject(s)
Environmental Monitoring , Water Pollutants, Chemical , Environmental Monitoring/methods , Rivers , Water Pollutants, Chemical/analysis , Principal Component Analysis , Water Quality , ChinaABSTRACT
Selecting indicators based on the characteristics and development trends of a given study area is essential for building a framework for assessing urban ecological security. However, few studies have focused on how to select the representative indicators systematically, and quantitative research is lacking. We developed an innovative quantitative modeling approach called the grey dynamic hierarchy analytic system (GDHAS) for both the procedures of indicator selection and quantitative assessment of urban ecological security. Next, a systematic methodology based on the GDHAS is developed to assess urban ecological security comprehensively and dynamically. This assessment includes indicator selection, driving force-pressure-state-impact-response (DPSIR) framework building, and quantitative evaluation. We applied this systematic methodology to assess the urban ecological security of Tianjin, which is a typical coastal super megalopolis and the industry base in China. This case study highlights the key features of our approach. First, 39 representative indicators are selected for the evaluation index system from 62 alternative ones available through the GDHAS. Second, the DPSIR framework is established based on the indicators selected, and the quantitative assessment of the eco-security of Tianjin is conducted. The results illustrate the following: urban ecological security of Tianjin in 2008 was in alert level but not very stable; the driving force and pressure subsystems were in good condition, but the eco-security levels of the remainder of the subsystems were relatively low; the pressure subsystem was the key to urban ecological security; and 10 indicators are defined as the key indicators for five subsystems. These results can be used as the basis for urban eco-environmental management.
Subject(s)
Conservation of Natural Resources/methods , Environment , Risk Assessment/methods , China , Cities , Environmental Monitoring , Fuzzy Logic , Models, TheoreticalABSTRACT
Chemical industry parks in China are considered high-risk areas because they present numerous risks that can damage the environment, such as pollution incidents. In order to identify the environmental risks and the principal risk factors in these areas, we have developed a simple physical model of a regional environmental risk field (ERF) using existing dispersal patterns and migration models. The regional ERF zoning was also conducted and a reference value for diagnostic methods was developed to determine risk-acceptable, risk-warning, and risk-mitigation zones, which can provide a risk source layout for chemical industry parks. In accordance with the environmental risk control requirements, this study focused on the three stages of control and management of environmental risk and established an environmental risk management system including risk source identification and assessment, environmental safety planning, early risk warning, emergency management, assessment of environmental effects, and environmental remediation of pollution accidents. By using this model, the environmental risks in Tianjin Binhai New Area, the largest chemical industry park in China, were assessed and the environmental risk zoning map was drawn, which suggested the existence of many unacceptable environmental risks in this area. Thus, relevant suggestions have been proposed from the perspective of the adjustment of risk source layout, intensified management of environmental risk control and so on.
Subject(s)
Chemical Industry/standards , Environmental Monitoring/methods , Environmental Pollutants/chemistry , Environmental Pollutants/toxicity , Risk Assessment , China , Ecosystem , Hazardous Substances/analysis , Hazardous Substances/toxicity , Humans , Industrial Waste/analysisABSTRACT
OBJECTIVE: To explore the effective therapeutic method in the treatment of idiopathic sudden hearing loss (ISHL). METHODS: One hundred and eighty-eight cases of ISHL were randomized into a warming-promoting needling group (74 cases), a conventional acupuncture group (56 cases) and a medication group (58 cases). In the conventional acupuncture group, the conventional needling technique was applied to Baihui (GV 20), Fengchi (GB 20), Yifeng (TE 17), Tinggong (SI 19), Touqiaoyin (GB 11) and Zhigou (TE 6) on the affected side. The treatment was given 5 times each week. Totally, the treatment of 6 weeks was required. In the warming-promoting needling group, on the basic treatment as the conventional acupuncture group, the warming-promoting needling technique was applied to Fengchi (GB 20). In the medication group, the intravenous drop with salvia injectio and mecobalamin was prescribed, once per day, for 10 days totally. Meanwhile, Erlong Zuoci Wan was prescribed for oral administration, 8 pills each time, three times a day for 30 days continuously. RESULTS: All of the three therapeutic methods achieved the effect on ISHL. The total effective rate was 89.2% (66/74) in the warming-promoting needling group, which was better than 62.5% (35/56) in the conventional acupuncture group and 53.4% (31/58) in the medication group (both P<0.01). CONCLUSION: The warming-promoting needling techinque achieves the significant efficacy on ISHL. The hearing improvement is superior to that treated with either the conventional needling technique or medication.
Subject(s)
Acupuncture Therapy , Hearing Loss, Sudden/therapy , Acupuncture Points , Acupuncture Therapy/instrumentation , Adult , Female , Humans , Male , Middle Aged , Needles , Treatment OutcomeABSTRACT
Comprehensive evaluation of the water environment for effective water quality management is complicated by a considerable number of factors and uncertainties. It is difficult to combine micro-evaluation with the macro-evaluation process. To effectively eliminate the subjective errors of the traditional analytic hierarchy process (AHP), a new modeling approach--the analytic hierarchy process and grey target theory (AHP-GTT) systematic model--is presented in this study to evaluate water quality in a certain watershed. A case study of applying the AHP-GTT systematic model to the evaluation and analysis of the water environment was conducted in the Yibin section of the Yangtze River, China. The micro-evaluation is based on defining the weights of indices of the water quality (IWQ) of each water cross-section, while the macro-evaluation is based on calculating the comprehensive indices of water environmental quality and analyzing the tendency of the water environment of each cross-section. The results indicated that the Baixi and Shuidongmen sections are seriously polluted areas, with the tendencies of becoming worse. Also, the key IWQs of these two cross-sections are 5-day biochemical oxygen demand and chemical oxygen demand of permanganate, respectively.