ABSTRACT
In this study, a response surface method (RSM) was used to optimise the ultrasonic-assisted deep eutectic solvent (DES) extraction of myricetin from myricetin leaves. The results demonstrated that the DES-5 (choline chloride-oxalic acid) system exhibited better extraction results than the other seven DESs prepared. The optimum extraction conditions for myricetin were a DES-5 system with 19% water content of DES, a liquid-to-solid ratio of 37 : 1 mL g-1, an extraction time of 45 min, and an extraction temperature of 72 °C. Under these conditions, the extraction amount of myricetin was 22.47 mg g-1. To optimise the extraction process, the crude myricetin extract was purified, and the optimal conditions were as follows: an AB-8 macroporous adsorption resin was used with an anhydrous ethanol desorption agent. The adsorption rate was 1 BV per h (bed volume per hour), the desorption rate was 1 BV per h, and the desorption capacity was 2 BV (bed volume). The antioxidant properties of the myricetin were also investigated. The results demonstrated that, with an increase in concentration, the scavenging rates of DPPH and ËOH free radicals increased. Compared to Vc, myricetin had a better scavenging ability for DPPH free radicals, whereas purified myricetin had a better antioxidant effect. At the same concentration, the radical-scavenging rate of the ËOH radical was slightly higher in myricetin purified by the macroporous adsorption resin than in Vc, and that of the unpurified myricetin was the smallest. Myricetin was purified using a macroporous adsorption resin to improve its antioxidant properties.
ABSTRACT
The aim of this study is to detect the action properties of functional materials (FM) in transforming waste into resource products with high humus content. FM (MnO2 and reducing sugar) were added in different periods of chicken manure composting. During composting, concentration of humic acids (HA) as aromatic fraction of humus, was increased by FM. The promotive effects of adding FM in later period was the most obvious. While adding FM in the beginning period could accelerate organic matter degradation, but it did not promote HA formation. Meanwhile, the microbial diversity was higher in groups by adding FM in the beginning and thermophilic periods. Therefore, it was speculated that FM might improve HA formation by promoting the abiotic polymerization of precursors. Eventually, structural equation model showed that FM was beneficial to abiotic pathway of HA formation. But the formation efficiency was reduced by interfering with biotic pathway.
Subject(s)
Composting , Animals , Chickens , Manganese Compounds , Manure , Oxides , SoilABSTRACT
The aim of this study is to reveal the roles of MnO2 in Maillard reaction of biotic composting, and to identify its effectiveness in promoting humus formation. Corn straw (CS) and chicken manure (CM) have been chosen to be composted. During CS composting, addition of MnO2 rapidly reduced reducing sugars concentration (decreased by 84.0%) in 5â¯days and significantly improved humus production by 38.7% compared with treatment without MnO2. Whereas in CM composting, the promoting effect of MnO2 on humus formation was relatively weak by comparing with the treatment group of CS. Additionally, the presence of MnO2 has reshaped bacteria community, which might be the reason of MnO2 stimulated bacteria to utilize organic matter during CM composting. Therefore, the structural equation modeling has confirmed that MnO2 mainly performed as chemical catalyst to promote humus formation during CS composting. Besides catalyst, MnO2 also played as a bioactive activator in CM composting.