ABSTRACT
Short association fibres (SAF) are the most abundant fibre pathways in the human white matter. Until recently, SAF could not be mapped comprehensively in vivo because diffusion weighted magnetic resonance imaging with sufficiently high spatial resolution needed to map these thin and short pathways was not possible. Recent developments in acquisition hardware and sequences allowed us to create a dedicated in vivo method for mapping the SAF based on sub-millimetre spatial resolution diffusion weighted tractography, which we validated in the human primary (V1) and secondary (V2) visual cortex against the expected SAF retinotopic order. Here, we extended our original study to assess the feasibility of the method to map SAF in higher cortical areas by including SAF up to V3. Our results reproduced the expected retinotopic order of SAF in the V2-V3 and V1-V3 stream, demonstrating greater robustness to the shorter V1-V2 and V2-V3 than the longer V1-V3 connections. The demonstrated ability of the method to map higher-order SAF connectivity patterns in vivo is an important step towards its application across the brain.
Subject(s)
Brain Mapping , Diffusion Tensor Imaging , Visual Cortex , Visual Pathways , Humans , Visual Cortex/physiology , Visual Cortex/diagnostic imaging , Male , Female , Adult , Diffusion Tensor Imaging/methods , Brain Mapping/methods , Visual Pathways/physiology , Visual Pathways/diagnostic imaging , White Matter/diagnostic imaging , White Matter/physiology , Young Adult , Image Processing, Computer-Assisted/methodsABSTRACT
Visual imagery and perception share neural machinery but rely on different information flow. While perception is driven by the integration of sensory feedforward and internally generated feedback information, imagery relies on feedback only. This suggests that although imagery and perception may activate overlapping brain regions, they do so in informationally distinctive ways. Using lamina-resolved MRI at 7 T, we measured the neural activity during imagery and perception of faces and scenes in high-level ventral visual cortex at the mesoscale of laminar organization that distinguishes feedforward from feedback signals. We found distinctive laminar profiles for imagery and perception of scenes and faces in the parahippocampal place area and the fusiform face area, respectively. Our findings provide insight into the neural basis of the phenomenology of visual imagery versus perception and shed new light into the mesoscale organization of feedforward and feedback information flow in high-level ventral visual cortex.
ABSTRACT
Interpretation of cortical laminar functional magnetic resonance imaging (fMRI) activity requires detailed knowledge of the spatiotemporal haemodynamic response across vascular compartments due to the well-known vascular biases (e.g. the draining veins). Further complications arise from the spatiotemporal hemodynamic response that differs depending on the duration of stimulation. This information is crucial for future studies using depth-dependent cerebral blood volume (CBV) measurements, which promise higher specificity for the cortical microvasculature than the blood oxygenation level dependent (BOLD) contrast. To date, direct information about CBV dynamics with respect to stimulus duration, cortical depth and vasculature is missing in humans. Therefore, we characterized the cortical depth-dependent CBV-haemodynamic responses across a wide set of stimulus durations with 0.9 mm isotropic spatial and 0.785 seconds effective temporal resolution in humans using slice-selective slab-inversion vascular space occupancy (SS-SI VASO). Additionally, we investigated signal contributions from macrovascular compartments using fine-scale vascular information from multi-echo gradient-echo (ME-GRE) data at 0.35 mm isotropic resolution. In total, this resulted in >7.5h of scanning per participant (n=5). We have three major findings: (I) While we could demonstrate that 1 second stimulation is viable using VASO, more than 12 seconds stimulation provides better CBV responses in terms of specificity to microvasculature, but durations beyond 24 seconds of stimulation may be wasteful for certain applications. (II) We observe that CBV responses show dilation patterns across the cortex. (III) While we found increasingly strong BOLD signal responses in vessel-dominated voxels with longer stimulation durations, we found increasingly strong CBV signal responses in vessel-dominated voxels only until 4 second stimulation durations. After 4 seconds, only the signal from non-vessel dominated voxels kept increasing. This might explain why CBV responses are more specific to the underlying neuronal activity for long stimulus durations.
ABSTRACT
Tract-tracing studies in primates indicate that different subregions of the medial temporal lobe (MTL) are connected with multiple brain regions. However, no clear framework defining the distributed anatomy associated with the human MTL exists. This gap in knowledge originates in notoriously low MRI data quality in the anterior human MTL and in group-level blurring of idiosyncratic anatomy between adjacent brain regions, such as entorhinal and perirhinal cortices, and parahippocampal areas TH/TF. Using MRI, we intensively scanned four human individuals and collected whole-brain data with unprecedented MTL signal quality. Following detailed exploration of cortical networks associated with MTL subregions within each individual, we discovered three biologically meaningful networks associated with the entorhinal cortex, perirhinal cortex, and parahippocampal area TH, respectively. Our findings define the anatomical constraints within which human mnemonic functions must operate and are insightful for examining the evolutionary trajectory of the MTL connectivity across species.
Subject(s)
Entorhinal Cortex , Temporal Lobe , Animals , Humans , Temporal Lobe/diagnostic imaging , Entorhinal Cortex/diagnostic imaging , Memory , Neuroimaging , Magnetic Resonance Imaging/methods , Hippocampus/anatomy & histologyABSTRACT
The characterization of cortical myelination is essential for the study of structure-function relationships in the human brain. However, knowledge about cortical myelination is largely based on post-mortem histology, which generally renders direct comparison to function impossible. The repeating pattern of pale-thin-pale-thick stripes of cytochrome oxidase (CO) activity in the primate secondary visual cortex (V2) is a prominent columnar system, in which histology also indicates different myelination of thin/thick versus pale stripes. We used quantitative magnetic resonance imaging (qMRI) in conjunction with functional magnetic resonance imaging (fMRI) at ultra-high field strength (7 T) to localize and study myelination of stripes in four human participants at sub-millimeter resolution in vivo. Thin and thick stripes were functionally localized by exploiting their sensitivity to color and binocular disparity, respectively. Resulting functional activation maps showed robust stripe patterns in V2 which enabled further comparison of quantitative relaxation parameters between stripe types. Thereby, we found lower longitudinal relaxation rates (R1) of thin and thick stripes compared to surrounding gray matter in the order of 1-2%, indicating higher myelination of pale stripes. No consistent differences were found for effective transverse relaxation rates (R2*). The study demonstrates the feasibility to investigate structure-function relationships in living humans within one cortical area at the level of columnar systems using qMRI.
Subject(s)
Electron Transport Complex IV , Visual Cortex , Animals , Humans , Electron Transport Complex IV/metabolism , Brain Mapping , Visual Cortex/physiology , Vision Disparity , Magnetic Resonance ImagingABSTRACT
The subthalamic nucleus (STN) is a small, subcortical brain structure. It is a target for deep brain stimulation, an invasive treatment that reduces motor symptoms of Parkinson's disease. Side effects of DBS are commonly explained using the tripartite model of STN organization, which proposes three functionally distinct subregions in the STN specialized in cognitive, limbic, and motor processing. However, evidence for the tripartite model exclusively comes from anatomical studies and functional studies using clinical patients. Here, we provide the first experimental tests of the tripartite model in healthy volunteers using ultra-high field 7 Tesla (T) functional magnetic resonance imaging (fMRI). Thirty-four participants performed a random-dot motion decision-making task with a difficulty manipulation and a choice payoff manipulation aimed to differentially affect cognitive and limbic networks. Moreover, participants responded with their left and right index finger, differentially affecting motor networks. We analysed BOLD signal in three subregions of the STN along the dorsolateral-ventromedial axis, identified using manually delineated high resolution anatomical images and based on a previously published atlas. Using these paradigms, all segments responded equally to the experimental manipulations, and the tasks did not provide evidence for the tripartite model.
Subject(s)
Deep Brain Stimulation , Parkinson Disease , Subthalamic Nucleus , Deep Brain Stimulation/methods , Humans , Magnetic Resonance Imaging/methods , Parkinson Disease/diagnostic imaging , Subthalamic Nucleus/diagnostic imagingABSTRACT
We present the first three-dimensional (3D) concordance maps of cyto- and fiber architecture of the human brain, combining histology, immunohistochemistry, and 7-T quantitative magnetic resonance imaging (MRI), in two individual specimens. These 3D maps each integrate data from approximately 800 microscopy sections per brain, showing neuronal and glial cell bodies, nerve fibers, and interneuronal populations, as well as ultrahigh-field quantitative MRI, all coaligned at the 200-µm scale to the stacked blockface images obtained during sectioning. These unprecedented 3D multimodal datasets are shared without any restrictions and provide a unique resource for the joint study of cell and fiber architecture of the brain, detailed anatomical atlasing, or modeling of the microscopic underpinnings of MRI contrasts.
Subject(s)
Brain , Magnetic Resonance Imaging , Brain/diagnostic imaging , Brain/pathology , Brain Mapping/methods , Humans , Magnetic Resonance Imaging/methods , Microscopy , Nerve FibersABSTRACT
In Parkinson's disease, the depletion of iron-rich dopaminergic neurons in nigrosome 1 of the substantia nigra precedes motor symptoms by two decades. Methods capable of monitoring this neuronal depletion, at an early disease stage, are needed for early diagnosis and treatment monitoring. Magnetic resonance imaging (MRI) is particularly suitable for this task due to its sensitivity to tissue microstructure and in particular, to iron. However, the exact mechanisms of MRI contrast in the substantia nigra are not well understood, hindering the development of powerful biomarkers. In the present report, we illuminate the contrast mechanisms in gradient and spin echo MR images in human nigrosome 1 by combining quantitative 3D iron histology and biophysical modeling with quantitative MRI on post mortem human brain tissue. We show that the dominant contribution to the effective transverse relaxation rate (R2*) in nigrosome 1 originates from iron accumulated in the neuromelanin of dopaminergic neurons. This contribution is appropriately described by a static dephasing approximation of the MRI signal. We demonstrate that the R2* contribution from dopaminergic neurons reflects the product of cell density and cellular iron concentration. These results demonstrate that the in vivo monitoring of neuronal density and iron in nigrosome 1 may be feasible with MRI and provide directions for the development of biomarkers for an early detection of dopaminergic neuron depletion in Parkinson's disease.
Subject(s)
Dopaminergic Neurons/chemistry , Iron/analysis , Magnetic Resonance Imaging/methods , Substantia Nigra/cytology , Aged, 80 and over , Biophysics , Ferritins/analysis , Humans , Male , Melanins/analysis , Middle Aged , Models, Neurological , Parkinson Disease/metabolism , Parkinson Disease/pathology , Software , Substantia Nigra/chemistryABSTRACT
The subcortical sensory pathways are the fundamental channels for mapping the outside world to our minds. Sensory pathways efficiently transmit information by adapting neural responses to the local statistics of the sensory input. The long-standing mechanistic explanation for this adaptive behaviour is that neural activity decreases with increasing regularities in the local statistics of the stimuli. An alternative account is that neural coding is directly driven by expectations of the sensory input. Here, we used abstract rules to manipulate expectations independently of local stimulus statistics. The ultra-high-field functional-MRI data show that abstract expectations can drive the response amplitude to tones in the human auditory pathway. These results provide first unambiguous evidence of abstract processing in a subcortical sensory pathway. They indicate that the neural representation of the outside world is altered by our prior beliefs even at initial points of the processing hierarchy.
Subject(s)
Sensory Receptor Cells/physiology , Adaptation, Physiological/physiology , Brain/diagnostic imaging , Brain/physiology , Female , Functional Neuroimaging , Humans , Magnetic Resonance Imaging , Male , Neural Pathways/physiology , Young AdultABSTRACT
Post mortem magnetic resonance imaging (MRI) studies on the human brain are of great interest for the validation of in vivo MRI. It facilitates a link between functional and anatomical information available from MRI in vivo and neuroanatomical knowledge available from histology/immunocytochemistry. However, linking in vivo and post mortem MRI to microscopy techniques poses substantial challenges. Fixation artifacts and tissue deformation of extracted brains, as well as co registration of 2D histology to 3D MRI volumes complicate direct comparison between modalities. Moreover, post mortem brain tissue does not have the same physical properties as in vivo tissue, and therefore MRI approaches need to be adjusted accordingly. Here, we present a pipeline in which whole-brain human post mortem in situ MRI is combined with subsequent tissue processing of the whole human brain, providing a 3-dimensional reconstruction via blockface imaging. To this end, we adapted tissue processing procedures to allow both post mortem MRI and subsequent histological and immunocytochemical processing. For MRI, tissue was packed in a susceptibility matched solution, tailored to fit the dimensions of the MRI coil. Additionally, MRI sequence parameters were adjusted to accommodate T1 and T2∗ shortening, and scan time was extended, thereby benefiting the signal-to-noise-ratio that can be achieved using extensive averaging without motion artifacts. After MRI, the brain was extracted from the skull and subsequently cut while performing optimized blockface imaging, thereby allowing three-dimensional reconstructions. Tissues were processed for Nissl and silver staining, and co-registered with the blockface images. The combination of these techniques allows direct comparisons across modalities.
ABSTRACT
Superficial white matter (SWM) contains the most cortico-cortical white matter connections in the human brain encompassing the short U-shaped association fibers. Despite its importance for brain connectivity, very little is known about SWM in humans, mainly due to the lack of noninvasive imaging methods. Here, we lay the groundwork for systematic in vivo SWM mapping using ultrahigh resolution 7 T magnetic resonance imaging. Using biophysical modeling informed by quantitative ion beam microscopy on postmortem brain tissue, we demonstrate that MR contrast in SWM is driven by iron and can be linked to the microscopic iron distribution. Higher SWM iron concentrations were observed in U-fiber-rich frontal, temporal, and parietal areas, potentially reflecting high fiber density or late myelination in these areas. Our SWM mapping approach provides the foundation for systematic studies of interindividual differences, plasticity, and pathologies of this crucial structure for cortico-cortical connectivity in humans.
ABSTRACT
Most fundamental cognitive processes rely on brain networks that include both cortical and subcortical structures. Studying such networks using functional magnetic resonance imaging (fMRI) requires a data acquisition protocol that provides blood-oxygenation-level dependent (BOLD) sensitivity across the entire brain. However, when using standard single echo, echo planar imaging protocols, researchers face a tradeoff between BOLD-sensitivity in cortex and in subcortical areas. Multi echo protocols avoid this tradeoff and can be used to optimize BOLD-sensitivity across the entire brain, at the cost of an increased repetition time. Here, we empirically compare the BOLD-sensitivity of a single echo protocol to a multi echo protocol. Both protocols were designed to meet the specific requirements for studying small, iron rich subcortical structures (including a relatively high spatial resolution and short echo times), while retaining coverage and BOLD-sensitivity in cortical areas. The results indicate that both sequences lead to similar BOLD-sensitivity across the brain at 7 âT.
Subject(s)
Brain/diagnostic imaging , Image Processing, Computer-Assisted/methods , Magnetic Resonance Imaging/methods , Adult , Echo-Planar Imaging/methods , Female , Humans , Male , Young AdultABSTRACT
Short association fibers (U-fibers) connect proximal cortical areas and constitute the majority of white matter connections in the human brain. U-fibers play an important role in brain development, function, and pathology but are underrepresented in current descriptions of the human brain connectome, primarily due to methodological challenges in diffusion magnetic resonance imaging (dMRI) of these fibers. High spatial resolution and dedicated fiber and tractography models are required to reliably map the U-fibers. Moreover, limited quantitative knowledge of their geometry and distribution makes validation of U-fiber tractography challenging. Submillimeter resolution diffusion MRI-facilitated by a cutting-edge MRI scanner with 300 mT/m maximum gradient amplitude-was used to map U-fiber connectivity between primary and secondary visual cortical areas (V1 and V2, respectively) in vivo. V1 and V2 retinotopic maps were obtained using functional MRI at 7T. The mapped V1-V2 connectivity was retinotopically organized, demonstrating higher connectivity for retinotopically corresponding areas in V1 and V2 as expected. The results were highly reproducible, as demonstrated by repeated measurements in the same participants and by an independent replication group study. This study demonstrates a robust U-fiber connectivity mapping in vivo and is an important step toward construction of a more complete human brain connectome.
Subject(s)
Connectome/methods , Diffusion Tensor Imaging/methods , Neurons/cytology , Visual Pathways/cytology , Adult , Female , Humans , Image Processing, Computer-Assisted/methods , MaleABSTRACT
In our environment, our senses are bombarded with a myriad of signals, only a subset of which is relevant for our goals. Using sub-millimeter-resolution fMRI at 7T, we resolved BOLD-response and activation patterns across cortical depth in early sensory cortices to auditory, visual and audiovisual stimuli under auditory or visual attention. In visual cortices, auditory stimulation induced widespread inhibition irrespective of attention, whereas auditory relative to visual attention suppressed mainly central visual field representations. In auditory cortices, visual stimulation suppressed activations, but amplified responses to concurrent auditory stimuli, in a patchy topography. Critically, multisensory interactions in auditory cortices were stronger in deeper laminae, while attentional influences were greatest at the surface. These distinct depth-dependent profiles suggest that multisensory and attentional mechanisms regulate sensory processing via partly distinct circuitries. Our findings are crucial for understanding how the brain regulates information flow across senses to interact with our complex multisensory world.
Subject(s)
Attention/physiology , Auditory Cortex/physiology , Visual Cortex/physiology , Acoustic Stimulation , Adult , Brain Mapping , Female , Humans , Magnetic Resonance Imaging , Male , Photic Stimulation , Visual Fields/physiology , Young AdultABSTRACT
Sensory thalami are central sensory pathway stations for information processing. Their role for human cognition and perception, however, remains unclear. Recent evidence suggests an involvement of the sensory thalami in speech recognition. In particular, the auditory thalamus (medial geniculate body, MGB) response is modulated by speech recognition tasks and the amount of this task-dependent modulation is associated with speech recognition abilities. Here, we tested the specific hypothesis that this behaviorally relevant modulation is present in the MGB subsection that corresponds to the primary auditory pathway (i.e., the ventral MGB [vMGB]). We used ultra-high field 7T fMRI to identify the vMGB, and found a significant positive correlation between the amount of task-dependent modulation and the speech recognition performance across participants within left vMGB, but not within the other MGB subsections. These results imply that modulation of thalamic driving input to the auditory cortex facilitates speech recognition.
Subject(s)
Auditory Pathways/physiology , Geniculate Bodies/physiology , Speech Perception , Brain Mapping , Female , Humans , Magnetic Resonance Imaging , Male , Young AdultABSTRACT
The human neocortex is organized radially into six layers which differ in their myelination and the density and arrangement of neuronal cells. This cortical cyto- and myeloarchitecture plays a central role in the anatomical and functional neuroanatomy but is primarily accessible through invasive histology only. To overcome this limitation, several non-invasive MRI approaches have been, and are being, developed to resolve the anatomical cortical layers. As a result, recent studies on large populations and structure-function relationships at the laminar level became possible. Early proof-of-concept studies targeted conspicuous laminar structures such as the stria of Gennari in the primary visual cortex. Recent work characterized the laminar structure outside the visual cortex, investigated the relationship between laminar structure and function, and demonstrated layer-specific maturation effects. This paper reviews the methods and in-vivo MRI studies on the anatomical layers in the human cortex based on conventional and quantitative MRI (excluding diffusion imaging). A focus is on the related challenges, promises and potential future developments. The rapid development of MRI scanners, motion correction techniques, analysis methods and biophysical modeling promise to overcome the challenges of spatial resolution, precision and specificity of systematic imaging of cortical laminae.
Subject(s)
Cerebral Cortex/anatomy & histology , Magnetic Resonance Imaging/methods , Neuroimaging/methods , HumansABSTRACT
INTRODUCTION: The polyphenol resveratrol has been suggested to exert beneficial effects on memory and the aging hippocampus due to calorie-restriction mimicking effects. However, the evidence based on human interventional studies is scarce. We therefore aimed to determine the effects of resveratrol on memory performance, and to identify potential underlying mechanisms using a broad array of blood-based biomarkers as well as hippocampus connectivity and microstructure assessed with ultra-high field magnetic resonance imaging (UHF-MRI). METHODS: In this double-blind, randomized controlled trial, 60 elderly participants (60-79 years) with a wide body-mass index (BMI) range of 21-37 kg/m2 were randomized to receive either resveratrol (200â¯mg/day) or placebo for 26 weeks (registered at ClinicalTrials.gov: NCT02621554). Baseline and follow-up assessments included the California Verbal Learning Task (CVLT, main outcome), the ModBent task, anthropometry, markers of glucose and lipid metabolism, inflammation and neurotrophins derived from fasting blood, multimodal neuroimaging at 3 and 7â¯T, and questionnaires to assess confounding factors. RESULTS: Multivariate repeated-measures ANOVA did not detect significant time by group effects for CVLT performance. There was a trend for preserved pattern recognition memory after resveratrol, while performance decreased in the placebo group (n.s., pâ¯=â¯0.07). Further exploratory analyses showed increases in both groups over time in body fat, cholesterol, fasting glucose, interleukin 6, high sensitive C-reactive protein, tumor necrosis factor alpha and in mean diffusivity of the subiculum and presubiculum, as well as decreases in physical activity, brain-derived neurotrophic factor and insulin-like growth factor 1â¯at follow-up, which were partly more pronounced after resveratrol. DISCUSSION: This interventional study failed to show significant improvements in verbal memory after 6 months of resveratrol in healthy elderly with a wide BMI range. A non-significant trend emerged for positive effects on pattern recognition memory, while possible confounding effects of unfavorable changes in lifestyle behavior, neurotrophins and inflammatory markers occurred. Our findings also indicate the feasibility to detect (un)healthy aging-related changes in measures of hippocampus microstructure after 6 months using 7T diffusion MRI. More studies incorporating a longer duration and larger sample size are needed to determine if resveratrol enhances memory performance in healthy older adults.
Subject(s)
Hippocampus/drug effects , Memory/drug effects , Resveratrol/administration & dosage , Aged , Brain Mapping , Double-Blind Method , Female , Hippocampus/anatomy & histology , Hippocampus/physiology , Humans , Magnetic Resonance Imaging , Male , Memory/physiology , Middle Aged , Neural Pathways/anatomy & histology , Neural Pathways/physiology , Neuropsychological Tests , Pattern Recognition, Physiological/drug effects , Pattern Recognition, Physiological/physiologyABSTRACT
PURPOSE: To improve a radial multi-slice 2D gradient- and spin-echo (GRASE) sequence and provide an appropriate image reconstruction technique for SAR-reduced high-resolution neuroimaging. METHODS: Additional readout gradients per radio-frequency (RF) refocusing allow for a reduced number of RF pulses. In this way, a specific absorption rate (SAR) reduction is achieved and the application at high-field systems becomes more feasible. A phase insensitive image reconstruction is proposed to reduce signal dropout artifacts originating from opposite readout polarities. In addition, the image reconstruction allows for the calculation of images with varying contrast from one measurement. RESULTS: Results obtained at 3T and 7T demonstrate a SAR-reduction of at least 66% for a single-slice experiment with radial GRASE. The reduced SAR is used for an increased spatial coverage without increasing the measurement time. Experiments at 3T and 7T showed that the visual image quality is comparable to standard TSE and GRASE sequences with the same measurement parameters. Using higher EPI factors and the presented image reconstruction, artifact-free images with a significant SAR-reduction can be achieved. CONCLUSION: Radial GRASE enables SAR-reduced acquisitions of high-resolution brain images with different contrasts from one measurement and is a promising sequence for high-field neuroimaging.
Subject(s)
Brain/diagnostic imaging , Magnetic Resonance Imaging , Neuroimaging/methods , Humans , Image Enhancement , Neuroimaging/instrumentationABSTRACT
Physical exercise has been suggested to improve cognitive performance through various neurobiological mechanisms, mediated by growth factors such as BDNF, IGF-I, and VEGF. Moreover, animal research has demonstrated that combined physical and cognitive stimulation leads to increased adult neurogenesis as compared to either experimental condition alone. In the present study, we therefore investigated whether a sequential combination of physical and spatial training in young, healthy adults elicits an additive effect on training and transfer gains. To this end, we compared the effects of (i) eight 20-minute sessions of cycling, (ii) sixteen 30-minute sessions of spatial training, (iii) a combination of both, and included (iv) a passive control cohort. We assessed longitudinal changes in cognitive performance, growth factor levels, and T1 relaxation of hippocampal subfields (acquired with 7 T MRI). While substantial physical and spatial training gains were elicited in all trained groups, longitudinal transfer changes did not differ between these groups. Notably, we found no evidence for an additive effect of sequential physical and spatial training. These results challenge the extrapolation from the findings reported in animals to young, healthy adults.
Subject(s)
Cognition , Exercise/physiology , Hippocampus/physiology , Intercellular Signaling Peptides and Proteins/metabolism , Neuronal Plasticity , Spatial Learning , Adolescent , Adult , Female , Humans , Longitudinal Studies , Male , Young AdultABSTRACT
OBJECTIVE: Cortical malformations are documented postmortem in speech processing areas of the dyslexic human brain. The goal of this pilot study was to find out if such anatomic anomalies can be detected noninvasively and in vivo. METHODS: We developed a reconstruction of left perisylvian cortex profiles at a resolution of 400 µm using T1 data acquired with ultra-high-field MRI at 7T. Cortical thickness, myelinated cortical thickness, and layer-wise myelination were then compared in 6 men with developmental dyslexia and 6 healthy controls matched for age, sex, handedness, education level, and nonverbal IQ. RESULTS: Compared to healthy controls, dyslexic individuals showed comparable cortical thickness (t[1,10] = 1.98, p = 0.311) but significantly increased myelinated cortical thickness ratio (t[1,10] = 3.85, p = 0.013, familywise error-corrected, Cohen d = 2.03), resulting in an area under the receiver operator characteristic curve of 0.944 (p = 0.010, standard error 0.067, 95% confidence interval 0.814-1). Moreover, T1 relaxation, especially in layer IV of the left auditory cortex, was also significantly increased (t[1,10] = 3.32, p = 0.043, familywise-error corrected, Cohen d = 1.67). CONCLUSIONS: Our findings provide critical insights into the neurobiological manifestation of the most common learning disorder and suggest that our approach might also shed new light on other neurodevelopmental disorders associated with cortical abnormalities.