Growth Stimulation of Durum Wheat and Common Buckwheat by Non-Thermal Atmospheric Pressure Plasma.

The grains of durum wheat (Triticum durum Desf.) and achenes of common buckwheat (Fagopyrum esculentum Moench) were tested after treatment with two sources of non-thermal atmospheric pressure plasma (DCSBD, MSDBD) with different treatment times (0, 3, 5, 10, 20, 30, and 40 s). The effect of these treatments was monitored with regard to the seed surface diagnostics (water contact angle-WCA, chemical changes by Fourier transform infrared spectroscopy-FTIR); twenty parameters associated with germination and initial seed growth were monitored. A study of the wettability confirmed a decrease in WCA values indicating an increase in surface energy and hydrophilicity depending on the type of seed, plasma source, and treatment time. Surface analysis by attenuated total reflectance FTIR (ATR-FTIR) showed no obvious changes in the chemical bonds on the surface of the plasma-treated seeds, which confirms the non-destructive effect of the plasma on the chemical composition of the seed shell. A multivariate analysis of the data showed many positive trends (not statistically significant) in germination and initial growth parameters. The repeated results for germination rate and root/shoot dry matter ratio indicate the tendency of plants to invest in underground organs. Durum wheat required longer treatment times with non-thermal plasma (10 s, 20 s) for germination and early growth, whereas buckwheat required shorter times (5 s, 10 s). The responses of durum wheat grains to the two non-thermal plasma sources used were equal. In contrast, the responses of buckwheat achenes were more favorable to MSDBD treatment than to DCSBD.

Effect of Arsenic Soil Contamination on Stress Response Metabolites, 5-Methylcytosine Level and CDC25 Expression in Spinach.

Experimental spinach plants grown in soil with (5, 10 and 20 ppm) arsenic (As) contamination were sampled in 21 days after As(V) contamination. Levels of As in spinach samples (from 0.31 ± 0.06 µg g-1 to 302.69 ± 11.83 µg g-1) were higher in roots and lower in leaves, which indicates a low ability of spinach to translocate As into leaves. Species of arsenic, As(III) and As(V), were represented in favor of the As (III) specie in contaminated variants, suggesting enzymatic arsenate reduction. In relation to predominant As accumulation in roots, changes in malondialdehyde levels were observed mainly in roots, where they decreased significantly with growing As contamination (from 11.97 ± 0.54 µg g-1 in control to 2.35 ± 0.43 µg g-1 in 20 ppm As). Higher values in roots than in leaves were observed in the case of 5-methylcytosine (5-mC). Despite that, a change in 5-mC by As contamination was further deepened in leaves (from 0.20 to 14.10%). In roots of spinach, expression of the CDC25 gene increased by the highest As contamination compared to the control. In the case of total phenolic content, total flavonoid content, total phenolic acids content and total antioxidant capacity were higher levels in leaves in all values, unlike the roots.

The Impact of Nutshell Biochar on the Environment as an Alternative Fuel or as a Soil Amendment.

Walnut, pistachio, and peanut nutshells were treated by pyrolysis to biochar and analyzed for their possible usage as fuels or soil fertilizers. All the samples were pyrolyzed to five different temperatures, i.e., 250 °C, 300 °C, 350 °C, 450 °C, and 550 °C. Proximate and elemental analyses were carried out for all the samples, as well as calorific value and stoichiometric analysis. For sample usage as a soil amendment, phytotoxicity testing was performed and the content of phenolics, flavonoids, tannin, juglone, and antioxidant activity were determined. To characterize the chemical composition of walnut, pistachio, and peanut shells, lignin, cellulose, holocellulose, hemicellulose, and extractives were determined. As a result, it was found that walnut shells and pistachio shells are best pyrolyzed at the temperature of 300 °C and peanut shells at the temperature of 550 °C for their use as alternative fuels. The highest measured net calorific value was in pistachio shells, which were biochar pyrolyzed at 550 °C, of 31.35 MJ kg-1. On the other hand, walnut biochar pyrolyzed at 550 °C had the highest ash share of 10.12% wt. For their use as soil fertilizers, peanut shells were the most suitable when pyrolyzed at 300 °C, walnut shells at 300 and 350 °C, and pistachio shells at 350 °C.

Arsenic Soil Contamination and Its Effects on 5-Methylcytosine Levels in Onions and Arsenic Distribution and Speciation.

Arsenic represents a serious health threat in localities with a high arsenic-polluted environment and can easily get into the human food chain through agronomy production in areas affected by arsenic contamination. Onion plants that were grown in controlled conditions in arsenic-contaminated soil (5, 10, and 20 ppm) were harvested 21 days after contamination. Arsenic levels (from 0.43 ± 0.03 µg g-1 to 1761.11 ± 101.84 µg g-1) in the onion samples were high in the roots and low in the bulbs and leaves, which is probably caused by a reduced ability of the onions to transport arsenic from roots to bulbs and leaves. Arsenic species As(V) and As(III) in As(V)-contaminated soil samples were represented strongly in favor of the As(III) species. This indicates the presence of arsenate reductase. Levels of 5-methylcytosine (5-mC) (from 5.41 ± 0.28% to 21.17 ± 1.33%) in the onion samples were also higher in the roots than in the bulbs and leaves. Microscopic sections of the roots were examined, and the most damage was found in the 10 ppm As variant. Photosynthetic parameters pointed to a significant decrease in photosynthetic apparatus activity and the deterioration of the physiological state of plants as arsenic content increased in the soil.

Properties of Biochar Derived from Tea Waste as an Alternative Fuel and Its Effect on Phytotoxicity of Seed Germination for Soil Applications.

Tea waste as a potential biofuel and bio fertilizer was analyzed. Samples were collected from various tea species and torrefied to five different temperatures. All samples were analyzed for their proximal composition and calorific value. From the results, stoichiometric properties were calculated. A phytotoxicity test was performed, and the germination index was measured. Tea waste torrefied at 350 °C may be suitable biofuel reaching the calorific value of 25-27 MJ kg-1, but with quite a high share of ash, up to 10%, which makes its use technically challenging and may lead to operating issues in a combustion chamber. The same biochar may be a suitable fertilizer for increasing the germination index, therefore, applicable to the soil. The non-torrefied sample and the sample treated at 250 °C are not suitable as fertilizers for being toxic. The total phenolic content in waste black tea was reduced from 41.26 to 0.21 mg g-1, depending on the torrefaction temperature. The total flavonoid content was also reduced from 60.49 to 0.5 mg g-1. The total antioxidant activity in the non-torrefied sample was 144 mg g-1, and after torrefaction at 550 °C, it was 0.82 mg g-1. The results showed that black tea waste residues have the potential for further use, for example, in agriculture as a soil amendment or as a potential biofuel.

Use of Spent Coffee Ground as an Alternative Fuel and Possible Soil Amendment.

Spent coffee ground is a massively produced coffee industry waste product whose reusage is beneficial. Proximate and ultimate and stochiometric analysis of torrefied spent coffee ground were performed and results were analyzed and compared with other research and materials. Spent coffee ground is a material with high content of carbon (above 50%) and therefore high calorific value (above 20 MJ·kg-1). Torrefaction improves the properties of the material, raising its calorific value up to 32 MJ·kg-1. Next, the phytotoxicity of the aqueous extract was tested using the cress test. The non-torrefied sample and the sample treated at 250 °C were the most toxic. The sample treated at 250 °C adversely affected the germination of the cress seeds due to residual caffeine, tannins and sulfur release. The sample treated at 350 °C performed best of all the tested samples. The sample treated at 350 °C can be applied to the soil as the germination index was higher than 50% and can be used as an alternative fuel with net calorific value comparable to fossil fuels.