IFN-γ-Preconditioned Human Gingival-Derived Mesenchymal Stromal Cells Inhibit Plasmacytoid Dendritic Cells via Adenosine.

Plasmacytoid dendritic cells (pDCs) are vital players in antiviral immune responses because of their high levels of IFN-α secretion. However, this attribute has also implicated them as critical factors behind the immunopathogenesis of inflammatory diseases, and no currently available therapy can efficiently inhibit pDCs' aberrant activation. Mesenchymal stromal cells (MSCs) possess stromal immunomodulatory functionality, regulating immune cell activation through several mechanisms, including the adenosinergic (CD39/CD73/adenosine) pathway. The IFN-γ preconditioning of bone marrow MSCs improves their inhibitory properties for therapy applications; however, isolating human gingival tissue-derived MSCs (hGMSCs) is more accessible. These cells have shown better immunomodulatory effects, yet the outcome of IFN-γ preconditioning and its impact on the adenosinergic pathway has not been evaluated. This study first validated the immunoregulatory properties of primary-cultured hGMSCs, and the results showed that IFN-γ preconditioning strengthens CD39/CD73 coexpression, adenosine production, and the regulatory properties of hGMSC, which were confirmed by describing for the first time their ability to reduce pDC activation and their IFN-α secretion and to increase the frequency of CD73+ pDC. In addition, when CD73's enzymatic activity was neutralized in hGMSCs, adenosine production and the IFN-γ preconditioning effect were restrained. This evidence might be applied to design hGMSCs- and adenosine-based immunotherapeutic strategies for treating inflammatory disorders that are associated with pDC overactivation.

Percepción del ambiente educativo en médicos internos de pregrado de la facultad de medicina y cirugía, URSE / Perception of the educational environment in pre-degree internal doctors of the faculty of medicine and surgery, URSE

INTRODUCCIÓN: El ambiente educativo hace referencia a condiciones tangibles e intangibles en una institución y está asociado a la satisfacción de los estudiantes. OBJETIVO: Determinar la percepción del ambiente educativo que tienen los médicos internos de pregrado de la Facultad de Medicina y Cirugía (URSE) durante el ciclo escolar 2018-2019. MATERIALES Y MÉTODOS: Estudio descriptivo-transversal, se empleó el cuestionario Postgraduate Hospital Education Environment (PHEEM) a médicos internos de pregrado, el análisis de los datos se realizó a través de estadística descriptiva mediante SPSS(R) y la base de datos fue elaborada a través de Excel(R). RESULTADOS: El cuestionario fue respondido por 124 médicos internos (mujeres: 44%, hombres: 56%; edad: 24.71 años), la puntuación global del PHEEM fue catalogado como un ambiente educacional excelente. CONCLUSIONES: El ambiente educativo es percibido como excelente, aún se debe mejorar el desarrollo de prácticas médico-quirúrgicas y el diagnóstico en los diversos servicios del internado, como aspecto negativo se observa la pobre rotación por el servicio de Medicina Familiar

INTRODUCTION: The educational environment refers to tangible and intangible conditions in an institution and is associated with student satisfaction. OBJECTIVE: To determine the perception of the educational environment that internal undergraduate doctors of the Faculty of Medicine and Surgery (URSE) have during the 2018-2019 school year. MATERIALS AND METHODS: Descriptive-cross-sectional study, the Postgraduate Hospital Education Environment (PHEEM) questionnaire was used to undergraduate internal physicians, the data analysis was performed through descriptive statistics using SPSS(R) and the database was prepared through Excel(R). RESULTS: The questionnaire was answered by 124 internal doctors (women: 44%, men: 56%; age: 24.71 years), the overall PHEEM score was classified as an excellent educational environment. CONCLUSIONS: The educational environment is perceived as excellent, the development of medical-surgical practices and diagnosis in the various boarding school services must still be improved, as a negative aspect, the poor rotation by the Family Medicine service is observed