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Strongyloides stercoralis is an important soil-transmitted helminth occurring world-wide and affecting 30-100 million people. Because many cases are asymptomatic and sensitive diagnostic methods are lacking, S. stercoralis infection is frequently underdiagnosed. The increasing incidence of autoimmune and wasting diseases and increased use of immunosuppressive agents, as well as the increased use of immunosuppressants and cytotoxic drugs, have increased S. stercoralis infection and their mortality. This review provides information about S. stercoralis epidemiology, life cycle, aetiology, pathology, comorbidities, immunology, vaccines, diagnosis, treatment, prevention, control and makes some recommendations for future prevention and control of this important parasite.
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BACKGROUND AND AIMS: The liver possesses a remarkable regenerative capacity in response to injuries or viral infections. Various growth factors and cytokines are involved in regulating liver regeneration. Prostaglandin D 2 , a pro-resolution lipid mediator, is the most abundant hepatic prostanoid. However, the role of prostaglandin D 2 in the injury-induced liver regeneration remains unclear. APPROACH AND RESULTS: Two-thirds partial hepatectomy (70% PH), massive hepatectomy (85% resection), and carbon tetrachloride-induced chronic injury were performed in mice to study the mechanisms of live regeneration. Hepatic prostaglandin D 2 production was elevated in mice after PH. Global deletion of D prostanoid receptor (DP) 1, but not DP2, slowed PH-induced liver regeneration in mice, as evidenced by lower liver weight to body weight ratio, less Ki67 + hepatocyte proliferation, and G2/M phase hepatocytes. In addition, DP1 deficiency, specifically in resident KCs, and not in endothelial cells or HSCs, retarded liver regeneration in mice after PH. Conversely, the overexpression of exogenous DP1 in KCs accelerated liver regeneration in mice. Mechanistically, DP1 activation promoted Wnt2 transcription in a PKA/CREB-dependent manner in resident KCs and mediated hepatocyte proliferation through Frizzled8/ß-catenin signaling. Adeno-associated virus vector serotype 8-mediated Frizzled8 knockdown in hepatocytes attenuated accelerated liver regeneration in KC-DP1 transgenic mice after PH. Treatment with the DP1 receptor agonist BW245C promotes PH-induced liver regeneration in mice. CONCLUSIONS: DP1 activation mediates crosstalk between KCs and hepatocytes through Wnt2 and facilitates liver regeneration. Hence, DP1 may serve as a novel therapeutic target in acute and chronic liver diseases.
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The deleterious impact of pollution point sources on the surrounding environment and human has long been a focal point of environmental research. When considering the local atmospheric dispersion of semi-volatile organic compounds (SVOCs) around the emission sites, it is essential to account the dynamic process for the gas/particle (G/P) partitioning, which involves the transition from an initial state to a steady state. In this study, we have developed a model that enables the prediction of the dynamic process for G/P partitioning of SVOCs, particularly considering the influence from emission. It is important to note that the dynamic processes of the concentrations of SVOCs in particle phase (CP) and in gas phase (CG) differ significantly. These differences arise due to the influence of two critical factors: particulate proportion of SVOCs in the emissions (Ï0) and octanol-air partitioning coefficient (KOA). The validity of our model was assessed by comparing its predictions of the extremum value of the G/P partitioning quotient (KP) with the results obtained from the steady-state model. Remarkably, the characteristic time (tC), used to evaluate the timescale required for SVOCs to reach steady state, demonstrated different variations with KOA for CP and CG. Additionally, the values of tC were quite different for CP and CG, which were markedly influenced by Ï0. For some SVOCs with high KOA values, it took approximately 35 h to reach steady state. Furthermore, it was found that the time to achieve 95 % of steady state (t95 ≈ 3tC) could reach approximately 105 h. This duration is sufficient for chemicals to disperse from their emission site to the surrounding areas. Therefore, it is crucial to consider the dynamic process of G/P partitioning in local atmospheric transport studies. Moreover, the influence of Ï0 should be incorporated into future investigations examining the dynamic process of G/P partitioning.
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Background: Nodal T-follicular helper cell lymphomas (nTFHLs) represent a new family of peripheral T-cell lymphomas (PTCLs), and comparative studies of their constituents are rare. Methods: This study retrospectively enrolled 10 patients with nTFHL-F and 30 patients with nTFHL-NOS diagnosed between December 2017 and October 2023 at six large comprehensive tertiary hospitals; 188 patients with nTFHL-AI were diagnosed during the same period at the First Affiliated Hospital of Zhengzhou University for comparison. Results: Compared with nTFHL-AI, nTFHL-NOS patients exhibited better clinical manifestations, lower TFH expression levels, and a lower Ki-67 index. However, no differences in clinicopathological features were observed between nTFHL-F and nTFHL-AI patients as well as nTFHL-NOS patients. According to the survival analysis, the median OS for patients with nTFHL-NOS, nTFHL-AI, and nTFHL-F were 14.2 months, 10 months, and 5 months, respectively, whereas the median TTP were 14 months, 5 months, and 3 months, respectively. Statistical analysis revealed differences in TTP among the three subtypes(P=0.0173). Among the population of patients receiving CHOP-like induction therapy, there were significant differences in the OS and TTP among the nTFHL-NOS, nTFHL-AI, and nTFHL-F patients (P=0.0134, P=0.0205). Both the GDPT and C-PET regimens significantly improved the ORR, OS, and PFS in nTFHL patients. Conclusion: There are significant differences in the clinical manifestations, pathology, and survival outcomes among the three subtypes of nTFHLs. However, further research with a larger sample size, and involving clinical pathology and molecular genetics is needed to determine the distinctive biological characteristics of these tumors.
Subject(s)
Lymphoma, T-Cell, Peripheral , Humans , Retrospective Studies , Lymphoma, T-Cell, Peripheral/drug therapy , Survival Analysis , T-Lymphocytes, Helper-Inducer/metabolism , China/epidemiologyABSTRACT
This study aims to investigate the diagnostic potential of IL-2 for PDAC and develop a method to improve the dendritic cell (DC) based vaccine against PDAC. The gene expression data and clinical characteristics information for 178 patients with PDAC were obtained from The Cancer Genome Atlas (TCGA). DCs were isolated from Human peripheral blood mononuclear cells (PBMCs) and were cultured in 4 different conditions. DCs were pulsed by tumor cell lysates or KRAS G12D1 - 23 peptide, and then used to activate T cells. The mixture of DCs and T cells were administered to xenograft mouse model through the tail vein. The infiltration of DCs and T cells were detected by immunohistochemistry. The generation of KRAS G12D mutation specific cytotoxic T cells was determined by in vitro killing assay. We observed that PDAC patients with higher IL-2 mRNA levels exhibited improved overall survival and increased infiltration of CD8 + T cells, NK cells, naïve B cells, and resting myeloid DCs in the tumor microenvironment. IL-2 alone did not enhance DC proliferation, antigen uptake, or apoptosis inhibition unless co-cultured with PBMCs. DCs co-cultured with PBMCs in IL-2-containing medium demonstrated the strongest tumor repression effect in vitro and in vivo. Compared to DCs obtained through the traditional method (cultured in medium containing GM-CSF and IL-4), DCs cultured with PBMCs, and IL-2 exhibited increased tumor infiltration capacity, potentially facilitating sustained T cell immunity. DCs cultured in the PBMCs-IL-2 condition could promote the generation of cytotoxic T cells targeting tumor cells carrying KRAS G12D mutation.
Subject(s)
Interleukin-2 , Pancreatic Neoplasms , Humans , Animals , Mice , Interleukin-2/metabolism , Dendritic Cells , Leukocytes, Mononuclear , Proto-Oncogene Proteins p21(ras)/genetics , T-Lymphocytes, Cytotoxic , Pancreatic Neoplasms/metabolism , Tumor MicroenvironmentABSTRACT
BACKGROUND: Macrophages and neutrophils are rapidly recruited around Schistosome eggs to form granulomas. Extracellular traps (ETs) of macrophages and neutrophils are part of the pathogen clearance armamentarium of leukocytes. Schistosome eggs possess the ability to resist attack by the host's immune cells and survive by employing various immune evasion mechanisms, including the release of extracellular vesicles (EVs). However, the specific mechanisms by which Schistosome egg-derived EVs (E-EVs) evade the immune response and resist attack from macrophage and neutrophil ETs remain poorly understood. In this study, we aimed to investigate the association between E-EVs and macrophage/neutrophil ETs. METHODS: EVs were isolated from the culture supernatant of S. japonicum eggs and treated macrophages and neutrophils with E-EVs and Sja-miR-71a. The formation of ETs was then observed. Additionally, we infected mice with S. japonicum, administered HBAAV2/9-Sja-miR-71a, and the formation of macrophage ETs (METs) and neutrophil ETs (NETs) in the livers was measured. Sema4D-knockout mice, RNA sequencing, and trans-well assay were used to clarify Sja-miR-71a in E-EVs inhibits METs and NETs formation via the Sema4D/ PPAR-γ/ IL-10 axis. RESULTS: Our findings revealed that E-EVs were internalized by macrophages and neutrophils, leading to the inhibition of METs and NETs formation. The highly expressed Sja-miR-71a in E-EVs targeted Sema4D, resulting in the up-regulation of IL-10 and subsequent inhibition of METs and NETs formation. Sema4D knockout up-regulated IL-10 expression and inhibited the formation of METs and NETs. Furthermore, we further demonstrated that Sja-miR-71a inhibits METs and NETs formation via the Sema4D/ PPAR-γ/ IL-10 axis. CONCLUSIONS: In summary, our findings provide new insights into the immune evasion abilities of Schistosome eggs by demonstrating their ability to inhibit the formation of METs and NETs through the secretion of EVs. This study enhances our understanding of the host-pathogen interaction and may have implications for the development of novel therapeutic approaches. Video Abstract.
Subject(s)
Extracellular Traps , Extracellular Vesicles , MicroRNAs , Schistosoma japonicum , Mice , Animals , Schistosoma japonicum/genetics , Interleukin-10 , Peroxisome Proliferator-Activated Receptors/metabolism , Neutrophils , Extracellular Vesicles/metabolism , MicroRNAs/genetics , MicroRNAs/metabolism , MacrophagesABSTRACT
This paper presents the optical design of a high-resolution double-grating spectrometer for extracting the multiple lines in the Stokes or anti-Stokes branch of the pure rotational Raman spectra of nitrogen. The spectrometer is composed of collimating and focusing mirrors, two reflective gratings, and a linear detector. The structural parameters were calculated using geometric configuration, dispersion, and aberrational theory, and conditions for first-order correction of keystone distortion with divergent grating illumination were derived. Based on this method, we simulated a spectrometer with a 16-channel linear array photomultiplier tube, resulting in uniformly distributed single-branch lines on each detector channel. The resolution reached 0.225 nm per channel, and the keystone distortion was less than 0.7%. The spectrometer avoids the interference of elastic signals by not detecting them, enabling the extraction of atmospheric temperature profiles via separated single-branch lines with high precision. Our design provides a promising solution to extract atmospheric temperature profiles for pure rotational Raman lidar.
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Liver fibrosis can occur in many chronic liver diseases, and no effective treatments are available due to the poorly characterized molecular pathogenesis. Semaphorin 4D (Sema4D) has immune functions and serves important roles in T cell priming. Here, we found that Sema4D was highly expressed in fibrotic liver, and the expression of Sema4D increased with hepatic stellate cells (HSCs) activation. Knockout of Sema4D alleviated liver fibrosis. Mechanistically, knockout of Sema4D alleviated liver fibrosis by suppressing the expression of AOX1 in retinol metabolism. Further investigation demonstrated that retinoic acid receptor α (RARA), an important nuclear receptor of retinoic acid, was reduced by Sema4D knockout during liver fibrogenesis. Sema4D knockout-mediated suppression of liver fibrosis was partly mediated by regulating the balance of Th1, Th2, Th17, and T-bet+Treg cells via inhibiting AOX1/RARA. Thus, targeting Sema4D may hold promise as a potential therapeutic approach for treating liver fibrosis.
Subject(s)
Liver Cirrhosis , Semaphorins , Animals , Humans , Male , Mice , Aldehyde Oxidase , Antigens, CD , Liver Cirrhosis/genetics , Mice, Knockout , Semaphorins/geneticsABSTRACT
Background and Aims: Aminoacyl-tRNA synthetases (ARSs) participate in tumor initiation and progression but their involvement in hepatocellular carcinoma (HCC) is not clear. This study aimed to investigate the prognostic value and underlying mechanisms of ARS in HCC. Methods: Data were obtained from The Cancer Genome Atlas (TCGA), International Cancer Genome Consortium, Gene Expression Omnibus and Human Protein Atlas databases. The prognostic model was constructed with the use of Cox regression and least absolute shrinkage and selection operator regression. Kaplan-Meier survival analysis, enrichment analysis, single sample gene set enrichment analysis and tumor mutation burden calculation were performed with R to evaluate the model and explore the underlying mechanism. Wilcoxon tests were used for comparisons between groups. Results: Aspartyl-tRNA synthetase 2 (DARS2), tyrosyl-tRNA synthetase 1 (YARS1) and cysteinyl-tRNA synthetase 2 (CARS2) were identified as prognostic biomarkers and enrolled in model construction. The area under receiver operating characteristic curve of the model was 0.775. The model was used to assign patients from TCGA into low- and high-risk groups. Those in the high-risk group had a worse prognosis (p<0.001). The clinical significance of the model was tested in different clinical subgroups. Genetic mutation analysis had a higher TP53 mutation frequency in high-risk group. Enrichment analysis and study of immune-related cells and molecules found that the high-risk group was characterized by immune-cell infiltration and immunosuppression states. Conclusions: A novel ARS family-based model of HCC prognosis was constructed. TP53 mutation frequency and immune-suppressive status accounted for a worse prognosis in patients included in the high-risk group.
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BACKGROUND: Small intestinal vascular malformations (angiodysplasias) are common causes of small intestinal bleeding. While capsule endoscopy has become the primary diagnostic method for angiodysplasia, manual reading of the entire gastrointestinal tract is time-consuming and requires a heavy workload, which affects the accuracy of diagnosis. AIM: To evaluate whether artificial intelligence can assist the diagnosis and increase the detection rate of angiodysplasias in the small intestine, achieve automatic disease detection, and shorten the capsule endoscopy (CE) reading time. METHODS: A convolutional neural network semantic segmentation model with a feature fusion method, which automatically recognizes the category of vascular dysplasia under CE and draws the lesion contour, thus improving the efficiency and accuracy of identifying small intestinal vascular malformation lesions, was proposed. Resnet-50 was used as the skeleton network to design the fusion mechanism, fuse the shallow and depth features, and classify the images at the pixel level to achieve the segmentation and recognition of vascular dysplasia. The training set and test set were constructed and compared with PSPNet, Deeplab3+, and UperNet. RESULTS: The test set constructed in the study achieved satisfactory results, where pixel accuracy was 99%, mean intersection over union was 0.69, negative predictive value was 98.74%, and positive predictive value was 94.27%. The model parameter was 46.38 M, the float calculation was 467.2 G, and the time length to segment and recognize a picture was 0.6 s. CONCLUSION: Constructing a segmentation network based on deep learning to segment and recognize angiodysplasias lesions is an effective and feasible method for diagnosing angiodysplasias lesions.
Subject(s)
Angiodysplasia , Capsule Endoscopy , Humans , Capsule Endoscopy/methods , Artificial Intelligence , Neural Networks, Computer , Predictive Value of Tests , Angiodysplasia/diagnosisABSTRACT
BACKGROUND: Angiostrongylus cantonensis (A. cantonensis) infection can induce acute inflammation, which causes meningoencephalitis and tissue mechanical injury to the brain. Parasite infection-induced microRNAs play important roles in anti-parasite immunity in non-permissive hosts. miR-101b-3p is highly expressed after A. cantonensis infection; however, the role of miR-101b-3p and the transcription regulation of miR-101b-3p in A. cantonensis infection remain poorly characterized. RESULTS: In the present study, we found that miR-101b-3p inhibition alleviated inflammation infiltration and pyroptosis in A. cantonensis infection. In addition, we found that CCAAT/enhancer-binding protein alpha (CEBPα) directly bound to the - 6-k to - 3.5-k region upstream of miR-101b, and CEBPα activated miR-101b-3p expression in microglia. These data suggest the existence of a novel CEBPα/miR-101b-3p/pyroptosis pathway in A. cantonensis infection. Further investigation verified that CEBPα promotes pyroptosis by activating miR-101b-3p expression in microglia, and microglial pyroptosis further promoted inflammation. CONCLUSIONS: Our results suggest that a CEBPα/miR-101b-3p/pyroptosis pathway may contribute to A. cantonensis infection-induced inflammation and highlight the pro-inflammatory effect of miR-101b-3p. Video Abstract.
Subject(s)
Angiostrongylus cantonensis , Meningoencephalitis , MicroRNAs , Animals , Mice , Angiostrongylus cantonensis/metabolism , CCAAT-Enhancer-Binding Protein-alpha , Inflammation , Microglia/metabolism , MicroRNAs/genetics , MicroRNAs/metabolism , PyroptosisABSTRACT
Soil salinity is known to improve cadmium (Cd) mobility, especially in arid soils. However, the mechanisms involved in how salt stress-associated metabolic profiles participate in mediating Cd transport in the soil-plant system remain poorly understood. This study was designed to investigate the effects of salinity-induced changes in soil metabolites on Cd bioavailability. Sodium salts in different combinations according to molar ratio (NaCl:Na2SO4=1:1; NaCl:Na2SO4:NaHCO3=1:2:1; NaCl:Na2SO4:NaHCO3:Na2CO3=1:9:9:1; NaCl:Na2SO4:NaHCO3:Na2CO3=1:1:1:1) were applied to the Cd-contaminated soils, which increased soil Cd availability by 22.36% and the Cd content in wheat grains by 36.61%, compared to the control. Salt stress resulted in soil metabolic reprogramming, which might explain the decreased growth of wheat plants and increased Cd transport from the soil into wheat tissues. For example, down-regulation of starch and sucrose metabolism reduced the production of sugars, which adversely affected growth; up-regulation of fatty acid metabolism allowed wheat plants to maintain a normal intracellular environment under saline conditions; up-regulation of the tricarboxylic acid (TCA) cycle was triggered, causing an increase in organic acid synthesis and the accumulation of organic acids, which facilitated the migration of soil Cd into wheat tissues. In summary, salt stress can facilitate Cd transport into wheat tissues by the direct effect of salt-based ions and the combined effect of altered soil physicochemical properties and soil metabolic profiles in Cd-contaminated soils.
Subject(s)
Soil Pollutants , Soil , Soil/chemistry , Cadmium/chemistry , Triticum/metabolism , Soil Pollutants/analysis , Sodium Chloride , Salt StressABSTRACT
Schistosomiasis is an important, neglected tropical disease. Schistosoma japonicum can evade host attacks by regulating the host's immunity, causing continuous infection. However, interactions between the host's immune system and S. japonicum are unclear. Our previous research found that the Sj16 protein isolated from S. japonicum has an anti-inflammatory effect in the host. However, the role of Sj16 in the regulation of host immunity in S. japonicum infection is not clear. Here, we applied the CRISPR/Cas9 technique to knockout Sj16 in S. japonicum eggs and investigated the effect of Sj16 in regulating host immunity. We found egg viability decreased after Sj16 knockout. In addition, we found granulomatous inflammation increased, the T-cell immune response enhanced and the immune microenvironment changed in mice model injected with Sj16-knockout eggs by tail vein. These findings suggested that S. japonicum could regulate host immunity through Sj16 to evade the host immune attack and cause continuous infection. In addition, we confirmed the application of CRISPR/Cas9-mediated gene reprogramming for functional genomics in S. japonicum.
Subject(s)
Schistosoma japonicum , Mice , Animals , Schistosoma japonicum/genetics , Gene Knockout Techniques , CRISPR-Cas Systems , Anti-Inflammatory Agents/metabolism , ImmunityABSTRACT
Current clinical needs require the development and use of rapid and effective diagnostic indicators to accelerate the identification of pneumonia and the process of microbiological diagnosis. MicroRNAs (miRNAs) in extracellular vesicles (EVs) have become attractive candidates for novel biomarkers to evaluate the presence and progress of many diseases. We assessed their performance as biomarkers of pneumonia. Patients were divided into the pneumonia group (with pneumonia) and the control group (without pneumonia). We identified and compared two upregulated miRNAs in EVs derived from bronchoalveolar lavage fluid (BALF-EVs) between the two groups (PmiR-17-5p = 0.009; PmiR-193a-5p = 0.031). Interestingly, in cell-debris pellets and EVs-free supernatants derived from bronchoalveolar lavage fluid (BALF-cell-debris pellets and BALF-EVs-free supernatants), total plasma, and EVs derived from plasma (plasma-EVs), the expression of miR-17-5p and miR-193a-5p showed no difference between pneumonia group and control group. In vitro experiments revealed that miR-17-5p and miR-193a-5p were strikingly upregulated in EVs derived from macrophages stimulated by lipopolysaccharide. MiR-17-5p (area under the curve, AUC: 0.753) and miR-193a-5p (AUC: 0.692) in BALF-EVs are not inferior to procalcitonin (AUC: 0.685) in the diagnosis of pneumonia. Furthermore, miR-17-5p and miR-193a-5p in BALF-EVs had a significantly higher specificity compared to procalcitonin and could be served as a potential diagnostic marker. MiR-17-5p and miR-193a-5p in EVs may be involved in lung inflammation by influencing the forkhead box O (FoxO) signaling pathway and protein processing in endoplasmic reticulum. This study is one of the few studies which focused on the potential diagnostic role of miRNAs in BALF-EVs for pneumonia and the possibility to use them as new biomarkers for a rapid and early diagnosis.
Subject(s)
Extracellular Vesicles , MicroRNAs , Pneumonia , Biomarkers/metabolism , Bronchoalveolar Lavage Fluid , Extracellular Vesicles/metabolism , Humans , Lipopolysaccharides/metabolism , MicroRNAs/metabolism , Pneumonia/diagnosis , Pneumonia/metabolism , Procalcitonin/metabolismABSTRACT
Trichinella spiralis (T. spiralis) is a globally distributed food-borne parasite that can coexist with the host for a long time after infection. Trichinella-derived secretions can regulate the immune response and fibroblasts of the host, but the specific mechanisms involved are still unclear. The purpose of this study was to investigate the role of T. spiralis larvae-derived extracellular vesicles (EVs) and their key miRNAs in the process of T. spiralis-host interaction. In this study, we found that the EVs of T. spiralis larvae, as well as miR-1-3p and let-7-5p, expressed in T. spiralis larvae-derived EVs, can promote the polarization of bone marrow macrophages to M2b type while inhibiting the activation of fibroblasts. These findings will contribute to further understanding of the molecular mechanisms underlying T. spiralis-host interactions.
Subject(s)
Extracellular Vesicles , MicroRNAs , Trichinella spiralis , Trichinellosis , Animals , Fibroblasts , Larva , Macrophages , Trichinellosis/parasitologyABSTRACT
The lack of toxicological data becomes the main bottleneck of ecological risk assessment of lead (Pb) in Chinese soils. The present study assessed Pb toxicity on three underground test endpoints (barley root elongation, earthworm avoidance response, and substrate-induced respiration (SIR) of microorganism) in 10 different soils. Hormetic dose-response induced by Pb was >118 % for earthworm avoidance response. EC10 and EC50 (the effective concentrations of Pb that inhibit 10 % or 50 % of endpoint bioactivity and also represents the toxicity threshold of Pb) after leaching increased by 0.32-8.73 times, and 1.02-3.75 times, respectively. Leaching factor (LF) prediction models indicated pH and cation exchange capacity (CEC) were the vital predictors for LF10 and LF50, explaining 60.6 % and 73.1 % of variations, respectively. SIR was one sensitive test endpoint for Pb toxicity, with the lowest of EC10 and EC50 values (from 373.7 to 1008.5 mg·kg-1, and from 837.1 to 2869.0 mg·kg-1, respectively). The best prediction models between ECx and soil properties is LogEC50 = 1.324Log(pH) + 0.423Log(CEC) + 1.742 (R2 = 0.761, p < 0.01). The results displayed significant implications for deriving ECx of Pb, and provided a scientific basis for soil ecological risk assessment of Pb.
Subject(s)
Oligochaeta , Soil Pollutants , Animals , Soil/chemistry , Soil Pollutants/analysis , Lead/toxicity , ChinaABSTRACT
The unsteady comprehensive system of peâ¯+â¯pH strongly affects the fate of Cd in paddy soils. However, the specific peâ¯+â¯pH threshold determining Cd bioavailability is largely unknown especially considering the roles of Fe and S reduction. The experiment set different water managements to obtain paddy soil samples with unstable peâ¯+â¯pH, and chemical analysis, X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS) characterization were applied to reveal the dynamic process and mechanism about how Fe and S controlled Cd mobilization. The results showed that low peâ¯+â¯pH was favorable to soil Cd immobilization. Compared with high and medium peâ¯+â¯pH, the exchangeable Cd content decreased by 67.57â¯% and 64.71â¯% at low peâ¯+â¯pH, respectively. The XPS results showed that the contents of Fe(II) and S(-II) increased to 65.1â¯% and 75.2â¯% at low peâ¯+â¯pH condition, which was higher than that in other treatments. In the process of flooding for reducing Cd mobility, first it was attributed to the formation of amorphous iron oxides that can provide amount of adsorption sites for Cd. After then, S2- began to play a dominant role to combine with Cd2+ to form CdS with continuous decreased peâ¯+â¯pH. Therefore, Fe and S played the different dominant roles on Cd immobilization in paddy soil, and soil peâ¯+â¯pH value could work as a threshold.
Subject(s)
Oryza , Soil Pollutants , Cadmium/analysis , Ferrous Compounds/metabolism , Hydrogen-Ion Concentration , Iron/analysis , Oryza/chemistry , Soil/chemistry , Soil Pollutants/analysis , Water/analysisABSTRACT
Aging of pollutants determines bioavailability and toxicity thresholds of environmental pollutants in soil. However, the ecotoxicity of chromium (Cr) rarely considers the effect of aging as well as soil properties. In order to explore the aging characteristics and establish their quantitative relationship with different soil properties, this study selected 7 soils with different properties through exogenous addition of Cr and determined its toxicity on barley root elongation. From 14d to 540d, EC10 and EC50 of barley root elongation ranged from 21.40 to 312.52 (mg·kg-1) and 50.15 to 883.88 (mg·kg-1) respectively. The hormesis appeared in the dose-response curve of acid soil as relative barley root elongation reached >110 % compared with the control. Extended aging time of Cr from 14d to 540d was associated with the attenuation of the toxicity of Cr, as the aging factor increased from 1.26 to 6.09 for EC50, from 0.88 to 4.98 for EC10. The prediction model of AFEC50 and soil properties is lg (AF360d) = 0.306lg Clay+0.026lg CEC + 0.240 (R2 = 0.872, P < 0.01). The results demonstrated that with the extension of aging time, the toxicity of Cr decreased at 360d and reached a slow reaction stage, after that soil OC, Clay and CEC could well explain the aging procedure of Cr (VI). These results are beneficial for risk assessment of Cr contaminated soils and establishment of a soil environmental quality criteria for Cr.
Subject(s)
Hordeum , Soil Pollutants , Chromium/toxicity , Clay , Soil , Soil Pollutants/analysisABSTRACT
Extra-nodal NK/T-cell lymphoma, nasal type (ENKTCL) is a highly aggressive Epstein-Barr virus associated lymphoma, typically presenting in the nasal and paranasal areas. We assembled a large series of ENKTCL (n = 209) for comprehensive genomic analysis and correlative clinical study. The International Lymphoma Prognostic Index (IPI), site of disease, stage, lymphadenopathy, and hepatomegaly were associated with overall survival. Genetic analysis revealed frequent oncogenic activation of the JAK/STAT3 pathway and alterations in tumor suppressor genes (TSGs) and genes associated with epigenomic regulation. Integrated genomic analysis including recurrent mutations and genomic copy number alterations using consensus clustering identified seven distinct genetic clusters that were associated with different clinical outcomes, thus constituting previously unrecognized risk groups. The genetic profiles of ENTKCLs from Asian and Hispanic ethnic groups showed striking similarity, indicating shared pathogenetic mechanism and tumor evolution. Interestingly, we discovered a novel functional cooperation between activating STAT3 mutations and loss of the TSG, PRDM1, in promoting NK-cell growth and survival. This study provides a genetic roadmap for further analysis and facilitates investigation of actionable therapeutic opportunities in this aggressive lymphoma.