Structural engineering of atomic catalysts for electrocatalysis.

As a burgeoning category of heterogeneous catalysts, atomic catalysts have been extensively researched in the field of electrocatalysis. To satisfy different electrocatalytic reactions, single-atom catalysts (SACs), diatomic catalysts (DACs) and triatomic catalysts (TACs) have been successfully designed and synthesized, in which microenvironment structure regulation is the core to achieve high-efficiency catalytic activity and selectivity. In this review, the effect of the geometric and electronic structure of metal active centers on catalytic performance is systematically introduced, including substrates, central metal atoms, and the coordination environment. Then theoretical understanding of atomic catalysts for electrocatalysis is innovatively discussed, including synergistic effects, defect coupled spin state change and crystal field distortion spin state change. In addition, we propose the challenges to optimize atomic catalysts for electrocatalysis applications, including controlled synthesis, increasing the density of active sites, enhancing intrinsic activity, and improving the stability. Moreover, the structure-function relationships of atomic catalysts in the CO2 reduction reaction, nitrogen reduction reaction, oxygen reduction reaction, hydrogen evolution reaction, and oxygen evolution reaction are highlighted. To facilitate the development of high-performance atomic catalysts, several technical challenges and research orientations are put forward.

Toxicity of common biocides used in aquaculture to embryos and larvae of Brachymystax tsinlingensis Li.

Brachymystax tsinlingensis Li is a threatened fish species endemic to China. With the problems of environmental factors and seeding breeding diseases, it is important to further improve the efficiency of seeding breeding and the basis of resource protection. This study investigated the acute toxicity of copper, zinc and methylene blue (MB) on hatching, survival, morphology, heart rate (HR) and stress behaviour of B. tsinlingensis. Eggs (diameter: 3.86 ± 0.07 mm, weight: 0.032 ± 0.004 g) of B. tsinlingensis were selected randomly from artificial propagation and developed from eye-pigmentation-stage embryos to yolk-sac stage larvae (length: 12.40 ± 0.02 mm, weight: 0.03 ± 0.001 g) and exposed to different concentrations of Cu, Zn and MB for 144 h in a series of semi-static toxicity tests. The acute toxicity tests indicated that the 96-h median lethal concentration (LC50 ) values of the embryos and larvae were 1.71 and 0.22 mg l-1 for copper and 2.57 and 2.72 mg l-1 for zinc, respectively, whereas the MB LC50 after 144-h exposure for embryos and larvae were 67.88 and 17.81 mg l-1 , respectively. The safe concentrations of copper, zinc and MB were 0.17, 0.77 and 6.79 mg l-1 for embryos and 0.03, 0.03 and 1.78 mg l-1 for larvae, respectively. Copper, zinc and MB treatments with concentrations greater than 1.60, 2.00 and 60.00 mg l-1 , respectively, led to a significantly low hatching rate and significantly high embryo mortality (P < 0.05), and copper and MB treatments with concentrations greater than 0.2 and 20 mg l-1 led to significantly high larvae mortality (P < 0.05). Exposure to copper, zinc and MB resulted in developmental defects, including spinal curvature, tail deformity, vascular system anomalies and discolouration. Moreover, copper exposure significantly reduced the HR of larvae (P < 0.05). The embryos exhibited an obvious change in behaviour, converting from the normal behaviour of emerging from the membrane head first to emerging tail first, with probabilities of 34.82%, 14.81% and 49.07% under copper, zinc and MB treatments, respectively. The results demonstrated that the sensitivity of yolk-sac larvae to copper and MB was significantly higher than that of embryos (P < 0.05) and that B. tsinlingensis embryos or larvae might be more resistant to copper, zinc and MB than other members of the Salmonidae family, which benefits their resource protection and restoration.

Effects of different combinations of koumine and gelsemine on growth performance, intestinal health, and transcriptome of Cyprinus carpio.

Koumine (KM) and gelsemine (GS) have shown significant benefits in livestock production, but their potential in aquaculture remains largely unexplored. This study examined the impact of different KM and GS combinations as feed additives on C. carpio (90 fish per group, initial weight 1.95 ± 0.08 g). KM and GS were introduced in ratios of 2:2 (mg/kg), 2:1 (mg/kg), and 2:0.67 (mg/kg) over a 10-week aquaculture experiment. The results demonstrate that the 2:1 (mg/kg) group increases the villus length, muscular layer thickness, crude protein, and crude fat content. Regarding fatty acid content, KM and GS enhance the levels of various fatty acids, including the total saturated fatty acid and total monounsaturated fatty acid. Additionally, KM and GS improve the composition and function of the intestinal microbiota. The 2:1 (mg/kg) group significantly elevates the enzymatic activities of SOD, MDA, CAT and upregulates the expression of immune-related genes such as toll-like receptor 2, transforming growth factor ß, and glutathione S-transferase. Transcriptomic analysis suggests that KM and GS may have potential benefits for nutrient utilization and immune regulation in C. carpio. In summary, this study provides valuable insights into the use of KM and GS as feed additives in aquaculture.

Evaluating effects of climate change on the spatial distribution of an atypical cavefish Onychostoma macrolepis.

Comprehending endangered species' spatial distribution in response to global climate change (GCC) is of great importance for formulating adaptive management, conservation, and restoration plans. However, it is regrettable that previous studies mainly focused on geoclimatic species, while neglected climate-sensitive subterranean taxa to a large extent, which clearly hampered the discovery of universal principles. In view of this, taking the endemic troglophile riverine fish Onychostoma macrolepis (Bleeker, 1871) as an example, we constructed a MaxEnt (maximum-entropy) model to predict how the spatial distribution of this endangered fish would respond to future climate changes (three Global Climate Models × two Shared Socio-economic Pathways × three future time nodes) based on painstakingly collected species occurrence data and a set of bioclimatic variables, including WorldClim and ENVIREM. Model results showed that variables related to temperature rather than precipitation were more important in determining the geographic distribution of this rare and endemic fish. In addition, the suitable areas and their distribution centroids of O. macrolepis would shrink (average: 20,901.75 km2) and move toward the northeast or northwest within the study area (i.e. China). Linking our results with this species' limited dispersion potential and unique habitat requirements (i.e. karst landform is essential), we thus recommended in situ conservation to protect this relict.

A New Porphyrin-based Covalent Organic Framework with High Iodine Capture Capacity and I-doping Enhanced Conductivity.

Covalent organic frameworks (COFs) are porous organic materials with well-defined and uniform structure. The material is an excellent candidate as a solid adsorbent for iodine adsorption. In the present study, we report the synthesis of COF with porphyrin moiety, TF-TA-COF, by solvothermal reaction, which was characterized by XRD, solid-state 13 C NMR, IR, TGA, and nitrogen adsorption-desorption analysis. TF-TA-COF showed a high specific surface area of 443 m2 g-1 , and exhibited good adsorption performance for iodine vapor, with an adsorption capacity of 2.74 g g-1 . XPS and Raman spectrum indicated that a hybrid of physisorption and chemisorption took place between host COF and iodine molecules. The electric properties of iodine-loaded TF-TA-COF were also studied. After doped with iodine, the conductivity of the material increased by more than 5 orders of magnitude. The photoconductivity of I2 -doped COF was also studied and TF-TA-COF showed doping-enhanced photocurrent generation.

Achievements and challenges of copper-based single-atom catalysts for the reduction of carbon dioxide to C2+ products.

Copper is the only metal that can convert CO2 into C2 and C2+ in electrocatalytic carbon dioxide reduction (CO2RR). However, the Faraday efficiency of CO2 conversion to C2 and C2+ products at high current densities is still low, which cannot meet the actual industrial demand. Here, the design methods of single-atom copper catalysts (including regulating the coordination environment of single-atom copper, modifying the carbon base surface and constructing diatomic Cu catalysts) are reviewed, and the current limitations and future research directions of copper-based single-atom catalysts are proposed, providing directions for the industrial conversion of CO2 into C2 and C2+ products.

Optimization strategies of high-entropy alloys for electrocatalytic applications.

High-entropy alloys (HEAs) are expected to become one of the most promising functional materials in the field of electrocatalysis due to their site-occupancy disorder and lattice order. The chemical complexity and component tunability make it possible for them to obtain a nearly continuous distribution of adsorption energy curve, which means that the optimal adsorption strength and maximum activity can be obtained by a multi-alloying strategy. In the last decade, a great deal of research has been performed on the synthesis, element selection and catalytic applications of HEAs. In this review, we focus on the analysis and summary of the advantages, design ideas and optimization strategies of HEAs in electrocatalysis. Combined with experiments and theories, the advantages of high activity and high stability of HEAs are explored in depth. According to the classification of catalytic reactions, how to design high-performance HEA catalysts is proposed. More importantly, efficient strategies for optimizing HEA catalysts are provided, including element regulation, defect regulation and strain engineering. Finally, we point out the challenges that HEAs will face in the future, and put forward some personal proposals. This work provides a deep understanding and important reference for electrocatalytic applications of HEAs.

Identification and characterization of long non-coding RNAs in intestinal immune regulation of largemouth bass, Micropterus salmoides, under acute heat stress.

Long noncoding RNAs (lncRNAs), which are RNA molecules that do not code for proteins and have a length exceeding 200 base pairs, have been found to play a crucial role in regulating intestinal immunity. The high mortality of various fish species induced by high temperatures is known to be associated with enteritis. Our investigation demonstrated that acute heat stress was responsible for inducing fish enteritis. However, the specific lncRNAs involved this process remains unknown. In this current study, we utilized intestinal sequencing data from the largemouth bass species Micropterus salmoides under acute heat stress, resulting in a total of 347,351,492 clean reads obtained from six cDNA libraries. A total of 3399 novel lncRNA transcripts originating from 2488 distinct lncRNA genes were successfully identified. Consistent with previous findings in other fish species, these lncRNAs demonstrated comparatively shorter transcript lengths when compared to protein-coding genes. Furthermore, a total of 216 novel lncRNA exhibited differential expression (DE) in the intestine of largemouth bass, meeting the criteria of absolute log2 fold change exceeding 2 and a p-value below 0.05. Additionally, these DE-lncRNAs were found to regulate 210 neighboring genes in a cis-regulatory manner. An examination of GO/KEGG enrichment revealed a notable enrichment of immune regulation (p < 0.05) among these cis-genes, with lncRNA MSTRG.8573.1 playing a significant role in regulating the jak-stat signaling pathway during this process. This study presents a comprehensive inventory of novel DE-lncRNA implicated in the development of enteritis in largemouth bass under acute heat stress. These findings offer valuable insights for future investigations on the regulation of lncRNAs to mitigate heat stress-induced fish enteritis.

Temporal correlations of ferroptosis, inflammation and oxidative stress under acute ammonia exposure in brain tissue of yellow catfish (Pelteobagrus fulvidraco).

Ammonia is one of the most serious environmental stressors which severely affect fishery production. Ammonia toxicity to fish has a tight relationship with oxidative stress, inflammation and ferroptosis (a type of programmed cell death characterized by iron-dependent lipid peroxidation), but the temporal response of the above three in brain remains unclear. In the present study, yellow catfish were exposed to three concentrations of ammonia: low concentration (TA-N ˂ 0.01 mg L-1, LA), middle concentration (TA-N 5.70 mg L-1, MA), high concentration (TA-N 28.50 mg L-1, HA) for 96 h. Brain was selected as target tissues for analysis. Results showed that ammonia stress resulted in firstly increased contents of hydroxyl radical at 1 h, total iron at 12 h, malondialdehyde at 48 h, respectively, and decreased contents of GSH at 3 h. The initial high expression levels of ferroptosis (GPX4, system xc-, TFR1) and inflammatory-related factors (NF-ƙB p65, TNF, COX-2, and LOX-15B), antioxidant enzymes genes (SOD and CAT) were observed at first hour upon MA or HA stress. Combining all, it suggested that brain ferroptosis and inflammation were the first to be activated at the initial stage of ammonia stress, and then that provoked oxidative stress.

Single-atom nanozyme-based electrochemical sensors for health and food safety monitoring.

Electrochemical sensors and biosensors play an important role in many fields, including biology, clinical trials, and food industry. For health and food safety monitoring, accurate and quantitative sensing is needed to ensure that there is no significantly negative impact on human health. It is difficult for traditional sensors to meet these requirements. In recent years, single-atom nanozymes (SANs) have been successfully used in electrochemical sensors due to their high electrochemical activity, good stability, excellent selectivity and high sensitivity. Here, we first summarize the detection principle of SAN-based electrochemical sensors. Then, we review the detection performances of small molecules on SAN-based electrochemical sensors, including H2O2, dopamine (DA), uric acid (UA), glucose, H2S, NO, and O2. Subsequently, we put forward the optimization strategies to promote the development of SAN-based electrochemical sensors. Finally, the challenges and prospects of SAN-based sensors are proposed.

Construction of highly-stable covalent organic framework with combined enol-imine and keto-enamine linkages.

A novel covalent organic framework (COF) (Tp-BI-COF) with combined ketimine-type enol-imine and keto-enamine linkages was prepared through a cascade of ketimine condensation followed by aldimine condensation and characterized by XRD, solid state 13C NMR, IR, TGA and BET. Tp-BI-COF showed high stability toward acid, organic solvent, and boiling water. The 2D COF exhibited photochromic properties after being irradiated with a xenon lamp. The stable COF, with aligned one-dimensional nanochannels, provided nitrogen sites on pore walls, which confine and stabilize the H3PO4 in the channel via hydrogen-bonding interactions. After loading with H3PO4, the material showed excellent anhydrous proton conductivity.

Insight into three water additives: Revealing the protective effects on survival and stress response under cold stress for Pacific white shrimp Litopenaeus vannamei.

The reproduction, development and growth of shrimp were hindered by cold stress, and even death was caused in severe cases. Moreover, huge economic losses to the shrimp aquaculture industry were caused every year by cold currents. The purpose of this study was to investigate the potential protective effects of water additives on the cold stress resistance of Pacific white shrimp (Litopenaeus vannamei) and their ability to improve the survival and stress response of the shrimp. Three potential cold-resistant additives adenosine triphosphate (A), soybean phospholipid (SP) and Clostridium butyricum (CB) on Pacific white shrimp under cold stress were added to the water with three concentrations for each additive. The mortality, activities of antioxidation enzymes and expression of anti-stress related genes in each group under cold stress were detected. The results showed that the cumulative mortality of low concentration for adenosine triphosphate (AL) and soybean phospholipid (SPL), medium concentration for soybean phospholipid (SPM) and high concentration for Clostridium butyricum (CBH) groups were significantly lower than that of the control (C) group when temperature maintained at 13 °C for 6 days. Total antioxidant capacity (T-AOC) content in shrimp plasma was significantly higher, while malondialdehyde (MDA) content was significantly lower than that in the C group. Gene expression analysis showed that 0.4 mg/L of adenosine triphosphate could regulate the immune defense ability and decrease apoptosis level of Pacific white shrimp under cold stress. Soybean phospholipid (2 mg/L) could enhance the immune ability of hepatopancreas, and Clostridium butyricum (10 mg/L) could significantly increase the expression of stress-related genes in shrimp intestine. Overall, these findings suggested that adenosine triphosphate and soybean phospholipid have the potential to be used as cold-resistant additives in Pacific white shrimp culture. This study provided valuable insights into addressing the problem of cold stress in shrimp culture.

Size dependent effects of nanoplastics and microplastics on the nitrogen cycle of microbial flocs.

NANO: and microplastics (NPs/MPs) are a new type of persistent environmental pollutant. Microbial flocs are a type of microbial aggregate commonly used in aquaculture. To investigate the impact of NPs/MPs on microbial flocs with different particle sizes: NPs/MPs-80 nm (M 0.08), NPs/MPs-800 nm (M 0.8), and NPs/MPs-8 µm (M 8), NPs/MPs exposure tests (28 days) and ammonia nitrogen conversion tests (24 h) were conducted. The results showed that the particle size was significantly higher in the M 0.08 group when compared with the control group (C group). The TAN (total ammonia nitrogen) content of each group maintained the order of M 0.08 > M 0.8 > M 8 > C from days 12-20. The nitrite content in the M 0.08 group was significantly higher on day 28 than that in the other groups. In the ammonia nitrogen conversion test, the nitrite content of the C group was significantly lower than that of the NPs/MPs exposure groups. The results suggested that NPs contributed to microbial aggregation and affected microbial colonization. In addition, NPs/MPs exposure could reduce microbial nitrogen cycling capacity, with a size-dependent toxicity difference of NPs > MPs. The findings of this study are expected to fill the research gap on the mechanisms of NPs/MPs' impact on microorganisms and the nitrogen cycle in aquatic ecosystems.

Responses of Micropterus salmoides under Ammonia Stress and the Effects of a Potential Ammonia Antidote.

Ammonia is a common environmental limiting factor in aquaculture. To investigate the effects of ammonia stress and explore the protective effect of N-carbamylglutamate (NCG) on Micropterus salmoides (M. salmoides), tissue sections and parameters related to oxidative stress and the inflammatory response in M. salmoides were carried out during the ammonia stress test and feeding test. The results demonstrated that the LC50 for 24 h, 48 h, 72 h, and 96 h under ammonia stress in M. salmoides were 25.78 mg/L, 24.40 mg/L, 21.90 mg/L, and 19.61 mg/L, respectively. Under ammonia stress, the structures of the tissues were damaged, and the GSH content decreased, while the MDA content increased with the increase in stress time and ammonia concentration. The NO content fluctuated significantly after the ammonia nitrogen stress. In the 15-day feeding test, with the increased NCG addition amount and feeding time, the GSH content increased while the MDA and NO contents decreased gradually in the NCG addition groups (NL group: 150 mg/kg; NM group: 450 mg/kg; NH group: 750 mg/kg) when compared with their control group (CK group: 0 mg/kg). In the ammonia toxicology test after feeding, the damage to each tissue was alleviated in the NL, NM, and NH groups, and the contents of GSH, MDA, and NO in most tissues of the NH group were significantly different from those in the CK group. The results suggested that ammonia stress caused tissue damage in M. salmoides, provoking oxidative stress and inflammatory response. The addition of NCG to the feed enhances the anti-ammonia ability of M. salmoides. Moreover, the gill and liver might be the target organs of ammonia toxicity, and the brain and kidney might be the primary sites where NCG exerts its effects. Our findings could help us to find feasible ways to solve the existing problem of environmental stress in M. salmoides culture.

New insights into the regulation mechanism of Litopenaeus vannamei hepatopancreas after lipopolysaccharide challenge using transcriptome analyses.

Litopenaeus vannamei (L. vannamei) is the most economically valuable cultured shrimp in the world, while Gram-negative bacteria infection causes huge economic losses to shrimp culture. In this study, we performed transcriptome sequencing of the hepatopancreas in L. vannamei after lipopolysaccharide (LPS, the cell wall component of Gram-negative bacteria) injection to investigate the response of shrimp under Gram-negative bacteria invasion. A total of 306 differentially expressed genes (DEGs) (70 up- and 236 down-regulated) were identified in the LPS treatment group (L group) when compared to their expression levels in the control group (C group). The oxidoreductase activity (GO:0016491) in the molecular function category was enriched in the LPS-responsive DEGs in GO annotation, and the metabolism of xenobiotics by cytochrome P450 (ko00980) was the most enriched pathway in KEGG annotation. The transcriptome profiling revealed that the toll like receptor, C-type lectin receptor, and ß-1,3-glucan binding protein were involved in the recognition of LPS during its early invasion stage. Although LPS could reduce the metabolic ability of exogenous substances, induce inflammation and reduce antioxidant capacity, L. vannamei could maintain its homeostasis by improving immunity, enhancing anti-stress ability and reducing apoptosis. Our research provides the first transcriptome profiling for the L. vannamei hepatopancreas after LPS injection. These results could offer a valuable reference on the mechanism of shrimp against Gram-negative bacteria and could provide guidance for shrimp farming.

Single and Combined Effects of Microplastics and Cadmium on the Cadmium Accumulation and Biochemical and Immunity of Channa argus.

Microplastics can accumulate residual drugs and heavy metals in the environment and accumulate through the layers of the food chain, ultimately causing harm to human health. The pollution of microplastics in the freshwater environment is becoming more and more serious, which directly affects the safety of aquatic organisms. This experiment studied the effects of single and composite microplastics and Cd on the tissue damage, antioxidant, and immune response of juvenile Channa argus. Microplastics with different diameters of 80 nm, 0.5 µm (200 µg/L), and Cd (50 µg/L) were used for exposure, and four sampling points were set for 24 h, 48 h, 96 h, and clear 48 h. Under different treatments, a certain degree of gill tissue damage can be found in 96 h. Microplastics and Cd can cause oxidative stress and affect the antioxidant status, and the impact of 0.5-µm microplastics is stronger than that of 80-nm microplastics. There is an antagonistic effect between the two microplastics and Cd during compound exposure, but the activity of CAT shows a synergistic effect. Microplastics and Cd affect the expression of immune-related genes to varying degrees. When exposed together, the expression of HSP70 gene all showed mutual antagonism, while the expression of IL-1ß gene was different. The expression of the MT gene can infer the ability of microplastics to accumulate Cd, and microplastics with a small diameter of 80 nm have stronger enrichment capabilities.

Enhanced oxygen evolution activity on mesoporous cobalt-iron oxides.

To solve the energy crisis and environmental pollution problems, the use of clean and renewable energy to replace fossil energy has become a top priority. The oxygen evolution reaction (OER) is the core of many renewable energy technologies. Developing low-cost and high-performance OER electrocatalysts is the key to implementing efficient energy conversion processes. Here, we synthesize ordered mesoporous iron-cobalt oxides using a hard template strategy. As a mesoporous oxide catalyst, meso-CoFe0.05Ox exhibits low OER overpotentials of 280 and 373 mV at current densities of 10 and 100 mA cm-2, respectively, and does not show deactivation for at least 18 hours at 100 mA cm-2. The introduction of iron can change the electronic structure of Co, and the orbital electrons are easily transferred from cobalt to iron. The enhanced OER performance can be attributed to concerted catalysis between the iron and cobalt sites that lowers the OER energy barrier, and the large specific surface area of the porous oxide providing efficient active sites for the reaction.

Delta Opioid Receptor Activation with Delta Opioid Peptide [d-Ala2, d-Leu5] Enkephalin Contributes to Synaptic Improvement in Rat Hippocampus against Global Ischemia.

Global cerebral ischemia induced by cardiac arrest usually leads to poor neurological outcomes. Numerous studies have focused on ways to prevent ischemic damage in the brain, however clinical therapies are still limited. Our previous studies revealed that delta opioid receptor (DOR) activation with [d-Ala2, d-Leu5] enkephalin (DADLE), a DOR agonist, not only significantly promotes neuronal survival on day 3, but also improves spatial memory deficits on days 5-9 after ischemia. However, the neurological mechanism underlying DADLE-induced cognitive recovery remains unclear. This study first examined the changes in neuronal survival in the CA1 region at the advanced time point (day 7) after ischemia/reperfusion (I/R) injury and found a significant amelioration of damaged CA1 neurons in the rats treated with DADLE (2.5 nmol) when administered at the onset of reperfusion. The structure and function of CA1 neurons on days 3 and 7 post-ischemia showed significant improvements in both the density of the injured dendritic spines and the basic transmission of the impaired CA3-CA1 synapses following DADLE treatment. The molecular changes involved in DADLE-mediated synaptic modulation on days 3 and 7 post-ischemia implied the time-related differential regulation of PKCα-MARCKS on the dendritic spine structure and of BDNF- ERK1/2-synapsin I on synaptic function, in response to ischemic/reperfusion injury as well as to DADLE treatment. Importantly, all the beneficial effects of DADLE on ischemia-induced cellular, synaptic, and molecular deficits were eliminated by the DOR inhibitor naltrindole (2.5 nmol). Taken together, this study suggested that DOR activation-induced protective signaling pathways of PKCα-MARCKS involved in the synaptic morphology and BDNF-ERK-synapsin I in synaptic transmission may be engaged in the cognitive recovery in rats suffering from advanced cerebral ischemia.

Insight into the immune and microbial response of the white-leg shrimp Litopenaeus vannamei to microplastics.

Microplastics (MPs) are a new type of environmental pollutant. To investigate the response of shrimp and their microflora to MPs, Litopenaeus vannamei (L. vannamei) was exposed to different concentrations of MPs (0, 50, 500, and 5000 µg/L, i.e., C, L, M and H groups) for 48 h. The survival rate, intake of MPs, immune-related gene expression and microbial response under MP exposure were detected. The results showed that the survival rate in the H group was significantly lower than those in the C, L and M groups, while the relative expression levels of proPO, TLR and ALF in the M and H groups were significantly higher than those in the C and L groups. For the microbial response, microbial community richness in the L group was significantly decreased, while community richness and diversity in the H group were significantly increased compared with those in the C group. The relative abundances of 3, 4 and 11 taxa were significantly changed after MP treatment at the phylum, class and genus levels, respectively. The results suggested that short-term exposure to low concentrations of MPs did not cause immune defense responses or death but affected the balance of bacterial composition in shrimp. Exposure to high concentrations of MPs can induce immune responses and microbial changes and can even cause death in shrimp. These findings increase our understanding of MP impacts on aquatic organisms.

Species-specific effect of microplastics on fish embryos and observation of toxicity kinetics in larvae.

Microplastics will definitely increase the potential health risks to humans through food chain, especially by commercial fishes. Here, we studied species-specific effect of microplastics on fish embryos and observed uptake, accumulation and elimination of microplastics in larvae. We chose three commercial fish species with different feeding types as our research objects. The results we found demonstrated that microplastics abundance in larvae was related with feeding type. At the same exposure concentration, the ingestion of microplastics in carnivores was lower than that in filter feeders and omnivores. In addition, omnivores were less able to remove microplastics than filter feeders. To the best of our knowledge, this is the first study to compared the differences of microplastics ingested in fishes with feeding types under laboratory conditions, and we believe that the findings will be valid evidence to explain species-specific effect of microplastics on fishes.