Postoperative chronic operation-related symptoms after minimally invasive lung surgery: a prospective observational protocol.

INTRODUCTION: Significant numbers of patients undergoing minimally invasive lung surgery develop chronic symptoms such as chronic pain and chronic cough after surgery, which may lead to a reduced quality of life (QoL). Despite this, there remains a dearth of high-quality prospective studies on this topic. Therefore, our study aims to systematically investigate the incidence and progression of long-term chronic symptoms following minimally invasive lung surgery, as well as changes in patient's psychological status and long-term QoL. METHODS: This is a single-centre, observational, prospective study that included patients with stage I non-small cell lung cancer or benign lesions. Prior to surgery, patients' baseline levels of chronic pain, chronic cough and sleep will be documented. Anxiety, depression and QoL assessments will be conducted using the Hospital Anxiety and Depression Scale (HADS) and the European Organisation for Research and Treatment of Cancer (EORTC) 30-item QoL Questionnaire (QLQ-C30). Following surgery, pain and cough will be evaluated during the initial 3 days using the Numeric Pain Rating Scale and Visual Analogue Scale score, with assessments performed thrice daily. Additionally, sleep status will be recorded daily during this period. Subsequently, postoperative chronic symptoms and QoL will be assessed at weeks 1, 2, 4, 12, 26 and 52. Chronic cough will be evaluated using the Leicester Cough Questionnaire, chronic pain will be assessed via the Brief Pain Inventory and McGill Pain Questionnaire while the EORTC QLQ-C30 questionnaire and HADS will provide continuous monitoring of QoL, anxiety and depression statuses. Data will also include the timing of chronic symptom onset, predisposing factors, as well as aggravating and relieving factors. ETHICS AND DISSEMINATION: Ethical approval was obtained from the Ethics Committees of Fujian Medical University Union Hospital. The findings will be disseminated in peer-reviewed publications. TRIAL REGISTRATION NUMBER: NCT06016881.

[Research Advances in the Association Between Alzheimer's Disease and Double-Stranded RNA-Dependent Protein Kinase].

Alzheimer's disease (AD) is a severe threat to human health and one of the three major causes of human death.Double-stranded RNA-dependent protein kinase (PKR) is an interferon-induced protein kinase involved in innate immunity.In the occurrence and development of AD,PKR is upregulated and continuously activated.On the one hand,the activation of PKR triggers an integrated stress response in brain cells.On the other hand,it indirectly upregulates the expression of ß-site amyloid precursor protein cleaving enzyme 1 and facilitates the accumulation of amyloid-ß protein (Aß),which could activate PKR activator to further activate PKR,thus forming a sustained accumulation cycle of Aß.In addition,PKR can promote Tau phosphorylation,thereby reducing microtubule stability in nerve cells.Inflammation in brain tissue,neurotoxicity resulted from Aß accumulation,and disruption of microtubule stability led to the progression of AD and the declines of memory and cognitive function.Therefore,PKR is a key molecule in the development and progression of AD.Effective PKR detection can aid in the diagnosis and prediction of AD progression and provide opportunities for clinical treatment.The inhibitors targeting PKR are expected to control the activity of PKR,thereby controlling the progression of AD.Therefore,PKR could be a target for the development of therapeutic drugs for AD.

Do Adverse Childhood Experiences Make Us More Utilitarian in Moral Dilemmas?

Purpose: Adverse childhood experiences (ACEs) have been associated with various aspects of morality, but their precise impact on moral decision-making remains unclear. This study aims to explore how ACEs influence moral decision-making in sacrificial dilemmas. Methods: Study 1 employed traditional dilemma analysis to quantify utilitarian responses and compare them among groups with no, low, and high ACEs. Study 2 utilized the CNI model to quantify three determinants of moral decision-making: sensitivity to consequences (C parameter), sensitivity to norms (N parameter), and general action tendencies (I parameter). Differences in these parameters among groups with no, low, and high ACEs were investigated. Results: Both Study 1 and Study 2 revealed that the high-ACE and low-ACE groups showed significantly higher utilitarian responses compared to the no-ACE group. However, no notable differences emerged between the high-ACE and low-ACE groups. Study 2 found that the N parameter was significantly lower in the high-ACE group compared to the low and no-ACE groups. Similarly, the low-ACE group exhibited significantly lower scores in the N parameter compared to the no-ACE group. Additionally, no significant differences were observed in the C and I parameters among groups with no, low, and high ACEs. Conclusion: These findings suggest that individuals with a high number of ACEs tend to exhibit more utilitarian responses, attributed to decreased affective response to the violation of moral rules, rather than increased deliberative cost-benefit reasoning or a general preference for action. Such insights deepen our understanding of the precise aspects of moral decision-making influenced by ACEs.

Contribution of FOS in neutrophils to venous thromboembolism via miR-144 based on bioinformatic prediction and validation.

The Finkel-Biskis-Jinkins Osteosarcoma (c-Fos; encoded by FOS) plays an important role in several cardiovascular diseases, including atherosclerosis and stroke. However, the relationship between FOS and venous thromboembolism (VTE) remains unknown. We identified differentially expressed genes in Gene Expression Omnibus dataset, GSE48000, comprising VTE patients and healthy individuals, and analysed them using CIBERSORT and weighted co-expression network analysis (WGCNA). FOS and CD46 expressions were significantly downregulated (FOS p = 2.26E-05, CD64 p = 8.83E-05) and strongly linked to neutrophil activity in VTE. We used GSE19151 and performed PCR to confirm that FOS and CD46 had diagnostic potential for VTE; however, only FOS showed differential expression by PCR and ELISA in whole blood samples. Moreover, we found that hsa-miR-144 which regulates FOS expression was significantly upregulated in VTE. Furthermore, FOS expression was significantly downregulated in neutrophils of VTE patients (p = 0.03). RNA sequencing performed on whole blood samples of VTE patients showed that FOS exerted its effects in VTE via the leptin-mediated adipokine signalling pathway. Our results suggest that FOS and related genes or proteins can outperform traditional clinical markers and may be used as diagnostic biomarkers for VTE.

A multimode biosensor based on prussian blue nanoparticles loaded with gold nanoclusters for the detection of aflatoxin B1.

Herein, a novel fluorescent/colorimetric/photothermal biosensor is proposed for aflatoxin B1 (AFB1) detection in food based on Prussian blue nanoparticles (PBNPs) (∼50 nm), gold nanoclusters (AuNCs), and an aptamer (Apt) within three hours. Briefly, a multifunctional compound, namely PBNPs-PEI@AuNCs, was synthesized from PBNPs as the loading carrier, polyethyleneimine (PEI) as the cross-linking agent, and AuNCs directly combined on the surface of PBNPs. The AFB1 Apt was then modified on the PBNPs-PEI@AuNCs to form a PBNPs-PEI@AuNCs-Apt probe, whereby when AFB1 is present, AFB1 is specifically captured by the probe. Meanwhile, the MNPs@antibody was also introduced to capture AFB1, thereby forming a "sandwich" structure compound. After magnetic separation, high temperature was applied to this "sandwich" structure compound to induce the denaturation of the Apt. Then the fluorescent/colorimetric/photothermal signals were collected from the PBNPs-PEI@AuNCs@Apt to give information on its related condition. The detection limits of the biosensor were 0.64 × 10-14, 0.96 × 10-14, and 0.55 × 10-12 g mL-1 for the three signals, which were outputted independently and could be verified with each other to ensure the accuracy of the results. Moreover, the colorimetric and photothermal strategies with this probe do not require large-scale instruments, providing a promising choice for achieving the rapid field detection of AFB1.

POSS and PEG Contained Copolymer for Antibioadhesive Rigid Contact Lenses Materials Application.

Myopia is a global public health issue. Rigid contact lenses (RCLs) are an effective way to correct or control myopia. However, bioadhesion issues remain one of the significant obstacles limiting its clinical application. Although enhancing hydrophilicity through various surface treatments can mitigate this problem, the duration of effectiveness is short-lived and the processing involved is complex and costly. Herein, an antiadhesive RCLs material was designed via 8-armed methacrylate-POSS (8MA-POSS), and poly(ethylene glycol) methacrylate (PEGMA) copolymerization with 3-[tris(trimethylsiloxy)silyl] propyl methacrylate (TRIS). The POSS and PEG segments incorporated P(TRIS-co-PEGMA-co-8MA-POSS) (PTPM) material was obtained and their optical transparency, refractive index, resolution, hardness, surface charge, thermal features, and wettability were tested and optimized. The antibioadhesion activities, including protein, lipid, and bacteria, were evaluated as well. In vitro and in vivo results indicated that the optimized antibioadhesive PTPM materials present good biocompatibility and biosafety. Thus, such POSS and PEG segments containing material were a potential antibioadhesive RCL material option.

Hsa_circ_0004872 alleviates meningioma progression by sponging miR-190a-3p/PTEN signaling.

BACKGROUND: Meningioma, the most prevalent intracranial tumor, possesses a significant propensity for malignant transformation. Circular RNAs (circ-RNAs), a class of non-coding RNAs, have emerged as crucial players in tumorigenesis. This study explores the functional relevance of hsa_circ_0004872, a specific circ-RNA, in the context of meningioma. METHODS: Molecular structure and stability of hsa_circ_0004872 were elucidated through PCR identification. Meningioma cell proliferation and apoptosis were assessed using the CCK-8 assay and flow cytometry, respectively. Gene and protein expression were analyzed via qRT-PCR and western blot. Molecular interactions were confirmed through dual-luciferase reporter gene and RIP assays. RESULTS: Hsa_circ_0004872, derived from exons 2 to 4 of the host gene MAPK1, demonstrated enhanced stability compared to its host MAPK1. Clinical data described that hsa_circ_0004872 was reduced in meningioma tissues and cell lines, and negatively correlated to poor survival rate of meningioma patients. Overexpression of hsa_circ_0004872 exhibited inhibitory effects on cell proliferation and promotion of apoptosis in vitro. Subsequent investigations unveiled a direct interaction between hsa_circ_0004872 and miR-190a-3p, leading to the activation of the PI3K/AKT signaling pathway through targeting PTEN. Notably, miR-190a-3p silence accelerated the apoptosis and proliferation inhibition of meningioma cells by inactivating PTEN/PI3K/AKT signaling, while miR-190a-3p overexpression showed an opposite effect, which greatly reversed the anti-tumor effects of hsa_circ_0004872 overexpression. CONCLUSION: In summary, our findings highlighted the intricate role of hsa_circ_0004872 in meningioma, shedding light on the regulatory mechanisms involving circ-RNAs in tumor progression. This positions hsa_circ_0004872 as a potential key regulatory factor in meningioma with implications for future therapeutic interventions.

Boosting Potassium Adsorption and Diffusion Performance of Carbon Anodes for Potassium-Ion Batteries via Topology and Curvature Engineering: From KT-Graphene to KT-CNTs.

We propose a two-dimensional carbon allotrope (named KT-graphene) by incorporating kagome and tetragonal lattices consisting of trigonal, quadrilateral, octagonal, and dodecagonal rings. The introduction of non-hexagonal rings can give rise to the localized electronic states that improve the chemical reactivity toward potassium, making KT-graphene a high-performance anode material for potassium-ion batteries. It shows a high theoretical capacity (892 mAh g-1), a low diffusion barrier (0.33 eV), and a low average open-circuit voltage (0.51 V). The presence of electrolyte solvents is propitious to boost the K-ion adsorption and diffusion capabilities. Moreover, one-dimensional nanotubes (KT-CNTs), rolled up by the KT-graphene sheet, are metallic regardless of the tube diameter. As the curvature increases, KT-CNTs exhibit significantly increased surface activity, which can promote the electron-donating ability of K. Furthermore, the curvature effect greatly enhances the efficiency of K diffusion on the inner surface compared to that on the outer surface.

Photo-controllable drug releasing bulk polyacrylic intraocular lens material for safer posterior capsular opacification prevention.

Posterior capsular opacification (PCO) is the most common complication that occurs after intraocular lens (IOL) implantation in cataract therapy. In recent years, IOLs have been developed as drug delivery platforms, but concerns over the safety of uncontrolled proliferative drug release have arisen. Therefore, a controlled drug release strategy is needed for safer PCO prevention. In this study, a new monomer contained coumarin group was introduced in material preparation, and poly(ethylene glycol phenyl ether methacrylate-co-2-(2-ethoxyethoxy) ethyl acrylate-co-7-(2-methacryloyloxyethoxy)-4-methylcoumarin) (PEEC) acrylic IOL materials were synthesized. The antiproliferative drug 5-fluorouracil (5-FU) could be chemically grafted to the PEEC IOL materials easily via a light induced [2 + 2] cycloaddition reaction with the coumarin group, getting drug-loaded IOL (PEEC@5-FU IOL). The PEEC@5-FU IOL exhibited excellent optical and mechanical properties and biocompatibility. More importantly, the loaded 5-FU could be easily controlled from release by light irradiation via photo-dissociation of the cyclobutane ring that was obtained by the [2 + 2] cycloaddition reaction of 5-FU and coumarin. The in vitro and in vivo experiments demonstrated that such photo-controllable drug release IOL could effectively prevent PCO after implantation in a safe way.

Mechanical method to reduce aberrations for a two-sided coating axisymmetric optical lens based on the specialized finite element method.

Two-sided coated optical lenses are important in optical applications. A film-stress-induced aberration can adversely affect the lens performance. In this paper, a mechanical method has been developed to reduce this aberration. The proposed method uses a specialized finite element method with an easy modeling process and high versatility to analyze the impact of film parameters (including stress, the thickness, and the coating range) on aberrations under different lens geometric parameters. Theoretically, by selecting the property film parameters within the range of an application's requirements can reduce the aberrations. The proposed method could reduce film-stress-induced aberrations to make the aberration compensation easier.

Surface modulation of zinc anodes by foveolate ZnTe nanoarrays for dendrite-free zinc ion batteries.

Zinc metal is widely considered as the primary option for constructing various aqueous batteries due to its cost-effectiveness, safety, and environmental friendliness. However, the Zn anode continues to be plagued by parasitic reactions and dendrite growth in aqueous electrolytes, limiting the practical implementation of zinc ion batteries (ZIBs) for large-scale energy storage. Herein, a foveolate ZnTe nanoarray is developed as a protective layer to enhance the chemical reversibility during Zn plating/stripping. The semi-conductive ZnTe with excellent ionic conductivity and hydrophobicity can effectually prevent the corrosion reactions, hydrogen generation and dendritic growth on the surface of the Zn anode. As a result, the Zn@ZnTe symmetrical cells achieve ultrahigh cycling stability (over 2800 h at 2 mA cm-2 and 1 mA h cm-2) and simultaneously deliver a low voltage hysteresis of 28 mV. Additionally, the durable Zn@ZnTe//V2O5 cells exhibit a remarkable capacity retention of 96.7% after 3000 cycles, surpassing that of the Zn//V2O5 cells. This work provides a straightforward and low-cost strategy to regulate the interface chemistry of the Zn anode, which may open a way for the development of practical ZIBs.

Efeitos de intervenções de enfermagem de alta qualidade nos resultados e na taxa de complicações de pacientes idosos submetidos à endoscopia gastrointestinal indolor / Efectos de intervenciones de enfermería de alta calidad en los resultados y en el índice de complicaciones de pacientes mayores sometidos a endoscopía gastrointestinal indolora / Effects of high-quality nursing interventions on outcomes and complication rate of elderly patients undergoing painless gastrointestinal endoscopy

Resumo Objetivo Para pacientes idosos, a endoscopia gastrointestinal indolor pode facilmente induzir eventos adversos. Nosso objetivo foi avaliar os efeitos de intervenções de enfermagem de alta qualidade nos resultados e na taxa de complicações de pacientes idosos submetidos à endoscopia gastrointestinal indolor. Métodos Foram selecionados 124 pacientes que receberam endoscopia gastrointestinal indolor no período de janeiro a dezembro de 2022, 62 dos quais internados entre janeiro e junho de 2022 foram incluídos em um grupo de enfermagem convencional e receberam intervenção de enfermagem básica tradicional, e os outros 62 internados entre julho e dezembro 2022 foram atribuídos a um grupo de enfermagem de alta qualidade e receberam intervenções de enfermagem de alta qualidade. Os resultados da intervenção foram comparados. Resultados As pontuações da Escala de Autoavaliação de Ansiedade e da Escala de Autoavaliação de Depressão do grupo de enfermagem de alta qualidade foram inferiores às do grupo de enfermagem convencional (P<0,05). A pressão arterial sistólica, a pressão arterial diastólica e a frequência cardíaca durante e após o diagnóstico e tratamento diminuíram no grupo de enfermagem de alta qualidade em comparação com os do grupo de enfermagem convencional (P <0,05). O grupo de enfermagem de alta qualidade teve uma taxa de complicação mais baixa e uma taxa de satisfação de enfermagem mais alta do que o grupo de enfermagem convencional (P<0,05). Conclusão A intervenção de enfermagem de alta qualidade não só estabiliza o estado psicológico dos pacientes idosos submetidos à endoscopia gastrointestinal indolor, mas também é benéfica para manter a pressão arterial e a frequência cardíaca estáveis.

Resumen Objetivo En pacientes mayores, la endoscopía gastrointestinal indolora puede inducir eventos adversos fácilmente. Nuestro objetivo fue evaluar los efectos de intervenciones de enfermería de alta calidad en los resultados y en el índice de complicaciones de pacientes mayores sometidos a endoscopía gastrointestinal indolora. Métodos Se seleccionaron 124 pacientes que recibieron endoscopía gastrointestinal indolora en el período de enero a diciembre de 2022. Entre ellos, se incluyó a 62 pacientes internados entre enero y junio de 2022 en un grupo de enfermería convencional y recibieron intervenciones de enfermería básica tradicional. Los otros 62 internados entre julio y diciembre de 2022 fueron asignados a un grupo de enfermería de alta calidad y recibieron intervenciones de enfermería de alta calidad. Se compararon los resultados de la intervención. Resultados Los puntajes de la Escala de Autoevaluación de Ansiedad y de la Escala de Autoevaluación de Depresión del grupo de enfermería de alta calidad fueron inferiores a los del grupo de enfermería convencional (p<0,05). La presión arterial sistólica, la presión arterial diastólica y la frecuencia cardíaca durante y después del diagnóstico y tratamiento disminuyeron en el grupo de enfermería de alta calidad en comparación con el grupo de enfermería convencional (p<0,05). El grupo de enfermería de alta calidad tuvo un índice de complicaciones más bajo y un índice de satisfacción de enfermería más alto que el grupo de enfermería convencional (p<0,05). Conclusión La intervención de enfermería de alta calidad no solo estabiliza el estado psicológico de los pacientes mayores sometidos a endoscopía gastrointestinal indolora, sino que también es benéfica para mantener la presión arterial y la frecuencia cardíaca estables.

Abstract Objective For elderly patients, painless gastrointestinal endoscopy can easily induce adverse events. We aimed to evaluate the effects of high-quality nursing interventions on the outcomes and complication rate of elderly patients undergoing painless gastrointestinal endoscopy. Methods A total of 124 patients who received painless gastrointestinal endoscopy from January to December 2022 were selected, 62 of whom admitted between January and June 2022 were included into a conventional nursing group and given traditional basic nursing intervention, and the other 62 admitted between July and December 2022 were assigned into a high-quality nursing group and given high-quality nursing intervention. The intervention outcomes were compared. Results The Self-rating Anxiety Scale and Self-rating Depression Scale scores of the high-quality nursing group were lower than those of the conventional nursing group (P<0.05). The systolic blood pressure, diastolic blood pressure, and heart rate during and after diagnosis and treatment decreased in the high-quality nursing group compared with those in the conventional nursing group (P<0.05). The high-quality nursing group had a lower complication rate and a higher nursing satisfaction rate than those of the conventional nursing group (P<0.05). Conclusion High-quality nursing intervention not only stabilizes the psychological state of elderly patients undergoing painless gastrointestinal endoscopy, but also is beneficial to maintaining stable blood pressure and heart rate.

Influence of subjective social class and social mobility beliefs on self-focused attention: the mediating role of sense of control.

We verified whether social class shapes different models of the self in China, by integrating individuals' social mobility beliefs and exploring the mediating effect of sense of control. Participants were randomly assigned to one of 2 (subjective social class: upper vs. low class) × 2 (social mobility beliefs: high vs. low mobility) manipulation conditions. They then completed the sense of control questionnaire and self-focused attention task. High mobility belief could alleviate the difference in perception among different subjective social classes and improve lower classes' control perception. Sense of control mediates subjective social class effects and social mobility beliefs on self-focused attention.

The Application of Wearable Sensors and Machine Learning Algorithms in Rehabilitation Training: A Systematic Review.

The integration of wearable sensor technology and machine learning algorithms has significantly transformed the field of intelligent medical rehabilitation. These innovative technologies enable the collection of valuable movement, muscle, or nerve data during the rehabilitation process, empowering medical professionals to evaluate patient recovery and predict disease development more efficiently. This systematic review aims to study the application of wearable sensor technology and machine learning algorithms in different disease rehabilitation training programs, obtain the best sensors and algorithms that meet different disease rehabilitation conditions, and provide ideas for future research and development. A total of 1490 studies were retrieved from two databases, the Web of Science and IEEE Xplore, and finally 32 articles were selected. In this review, the selected papers employ different wearable sensors and machine learning algorithms to address different disease rehabilitation problems. Our analysis focuses on the types of wearable sensors employed, the application of machine learning algorithms, and the approach to rehabilitation training for different medical conditions. It summarizes the usage of different sensors and compares different machine learning algorithms. It can be observed that the combination of these two technologies can optimize the disease rehabilitation process and provide more possibilities for future home rehabilitation scenarios. Finally, the present limitations and suggestions for future developments are presented in the study.

Assessment System for Child Head Injury from Falls Based on Neural Network Learning.

Toddlers face serious health hazards if they fall from relatively high places at home during everyday activities and are not swiftly rescued. Still, few effective, precise, and exhaustive solutions exist for such a task. This research aims to create a real-time assessment system for head injury from falls. Two phases are involved in processing the framework: In phase I, the data of joints is obtained by processing surveillance video with Open Pose. The long short-term memory (LSTM) network and 3D transform model are then used to integrate key spots' frame space and time information. In phase II, the head acceleration is derived and inserted into the HIC value calculation, and a classification model is developed to assess the injury. We collected 200 RGB-captured daily films of 13- to 30-month-old toddlers playing near furniture edges, guardrails, and upside-down falls. Five hundred video clips extracted from these are divided in an 8:2 ratio into a training and validation set. We prepared an additional collection of 300 video clips (test set) of toddlers' daily falling at home from their parents to evaluate the framework's performance. The experimental findings revealed a classification accuracy of 96.67%. The feasibility of a real-time AI technique for assessing head injuries in falls through monitoring was proven.

CRISPR/Cas12a and G-quadruplex DNAzyme-driven multimodal biosensor for visual detection of Aflatoxin B1.

Aflatoxin B1 (AFB1) contamination severely threatens human and animal health, it is thus critical to construct a strategy for its rapid, accurate, and visual detection. Herein, a multimodal biosensor was proposed based on CRISPR/Cas12a cleaved G-quadruplex (G4) for AFB1 detection. Briefly, specific binding of AFB1 to the aptamer occupied the binding site of the complementary DNA (cDNA), and cDNA then activated Cas12a to cleave G4 into fragments. Meanwhile, the intact G4-DNAzyme could catalyze 3, 3', 5, 5'-tetramethylbenzidine (TMB) to form colourimetric/SERS/fluorescent signal-enhanced TMBox, and the yellow solution produced by TMBox under acidic conditions could be integrated with a smartphone application for visual detection. The colourimetric/SERS/fluorescent biosensor yielded detection limits of 0.85, 0.79, and 1.65 pg·mL-1, respectively, and was applied for detecting AFB1 in peanut, maize, and badam samples. The method is suitable for visual detection in naturally contaminated peanut samples and has prospective applications in the food industry.

Effect of Sandpaper Meshes on the Performance of Tilia Sp. Self-Repairing Coatings.

This study aimed to investigate the impact of different sandpaper sanding meshes on the mechanical and optical properties of microencapsulated Tilia sp. film. An orthogonal experiment revealed that sanding between primers had the most significant effect. Furthermore, an independent experiment implied that increasing the mesh size resulted in decreased surface roughness and decreased color difference, elongation at break, and gloss after liquid resistance. In the aging test, the color difference of the paint film increased with the aging time, and the gloss tended to stabilize. Additionally, the anti-aging gloss of 240 mesh sandpaper used between primers remained relatively stable. The paint film sanded with 240 mesh sandpaper between primers displayed small and regular cracks after temperature and UV aging. Overall, the paint film demonstrated good comprehensive performance when sanded with 240 mesh between primers, 240 mesh between primer/topcoat, and 1000 mesh for topcoat. Self-repairing microcapsules showed better repair efficacy on the coating. This study provides a technical reference for the development of self-repairing coatings.

Effects of Alexithymia on Moral Decision-Making in Sacrificial Dilemmas: High Alexithymia is Associated with Weaker Sensitivity to Moral Norms.

Purpose: Although several studies have investigated the association between alexithymia and moral decision-making in sacrificial dilemmas, the evidence remains mixed. The current work investigated this association and how alexithymia affects moral choice in such dilemmas. Methods: The current research used a multinomial model (ie, CNI model) to disentangle (a) sensitivity to consequences, (b) sensitivity to moral norms, and (c) general preference for inaction versus action irrespective of consequences and norms in responses to moral dilemmas. Results: Higher levels of alexithymia were associated with a greater preference for utilitarian judgments in sacrificial dilemmas (Study 1). Furthermore, individuals with high alexithymia showed significantly weaker sensitivity to moral norms than did those with low alexithymia, whereas there were no significant differences in sensitivity to consequences or a general preference for inaction versus action (Study 2). Conclusion: The findings suggest that alexithymia affects moral choice in sacrificial dilemmas by blunting emotional reactions to causing harm, rather than through increased deliberative cost-benefit reasoning or general preference for inaction.

A novel camptothecin derivative, ZBH-01, exhibits superior antitumor efficacy than irinotecan by regulating the cell cycle.

BACKGROUND: Irinotecan (CPT-11) is a classic chemotherapeutic agent that plays an important role in the clinical treatment of metastatic colon cancer and other malignant tumors. We previously designed a series of novel irinotecan derivatives. In this study, we select one representative, ZBH-01, to investigate its sophisticated antitumor mechanism in colon tumor cells. METHODS: The cytotoxic activity of ZBH-01 on colon cancer cells was evaluate by MTT or Cell Counting Kit-8 (CCK8) assay, 3D and xenograft model. The inhibitory effect of ZBH-01 on TOP1 was detected by DNA relaxation assay and Immuno Complex of Ezyme (ICE) bioassay. The molecular mechanism of ZBH-01 was explored by Next-Generation Sequencing (NGS), bioinformatics analyses, flow cytometry, qRT-PCR, and western blot etc. RESULTS: ZBH-01 can induce obvious DNA damage and has superior antitumor activity against colon cancer cells compared to CPT-11 and SN38 (7-Ethyl-10-hydroxy camptothecin, the in vivo active form of CPT-11) both in vivo and in vitro. Its inhibitory effect on topoisomerase I (TOP1) was also comparable with these two control drugs. There are a much larger number of 842 downregulated and 927 upregulated mRNAs in ZBH-01 treatment group than that in the controls. The most significantly enriched KEGG pathways for these dysregulated mRNAs were DNA replication, the p53 signaling pathway, and the cell cycle. After constructing a protein-protein interaction (PPI) network and screening out a prominent cluster, 14 involved in the cell cycle process was identified. Consistently, ZBH-01 induced G0/G1 phase arrest in colon cancer cells, while CPT-11/SN38 caused S phase arrest. The initiation of apoptosis by ZBH-01 was also superior to CPT-11/SN38, followed by the increased expression of Bax, active caspase 3, and cleaved-PARP, and decreased expression of Bcl-2. Additionally, CCNA2 (cyclin A2), CDK2 (cyclin-dependent kinase 2), and MYBL2 (MYB proto-oncogene like 2) might be involved in the G0/G1 cell cycle arrest induced by ZBH-01. CONCLUSIONS: ZBH-01 can be an antitumor candidate drug for preclinical study in the future.

Platelet-to-Lymphocyte Ratio (PLR) as the Prognostic Factor for Recurrence/Residual Disease in HSIL Patients After LEEP.

Purpose: The platelet-to-lymphocyte ratio (PLR) is considered correlated with cancer prognosis including cervical cancer, in addition to high-risk papillomavirus (HR-HPV) infection, of which the predictive value in prognosis of high-grade squamous intraepithelial lesions (HSILs) remains unknown. Here, the prognostic predictive value of PLR in HSIL after loop electrosurgical excision procedure (LEEP) was evaluated. Patients and Methods: This study included 335 nonpregnant participants with histopathologically confirmed HSIL and 3- and 5-year follow-ups from the Fujian Cervical Lesions Screening Cohorts (FCLSCs) between September 2016 and September 2018. PLR and other variables were evaluated to identify the factors related to the recurrence/residual cervical intraepithelial neoplasia (CIN)-free survival (RFS), namely, the time from LEEP at baseline to first detection of recurrence/residual CIN or end of follow-up, by logistic and Cox regression. Results: In the Kaplan‒Meier analysis, HR-HPV infection (p=0.049/0.012), higher PLR (p=0.031/0.038), and gland invasion (p=0.047) had a higher risk for recurrence/residual CIN at the 3-/5-year follow-up. The univariate logistic and Cox regression analyses showed significant differences and a higher cumulative risk in patients with HR-HPV infection (OR=3.917, p=0.026; HR=3.996, p=0.020) and higher PLR (OR=2.295, p=0.041; HR=2.161, p=0.030) at the 5-year follow-up. The findings by multivariate Cox regression analysis were similar, indicating a poor prognosis for patients with HR-HPV infection (HR=3.901, p=0.023) and higher PLR (HR=2.082, p=0.038) at the 5-year follow-up. The calibration plot showed a better model fit for RFS at the 3-year follow-up. Conclusion: Preoperative PLR level and HR-HPV infection could be available markers for predicting recurrence/residual disease of HSIL after LEEP. Clinically, combining PLR with HR-HPV tests may provide novel evaluation method and reference for management in post-treatment patients with cervical precancerous lesions.