ABSTRACT
Here we develop a near infrared (NIR) fluorogenic probe for carbon monoxide (CO) detection and imaging based on palladium-mediated carbonylation using a NIR boron-dipyrromethene difluoride as a fluorophore and tetraethylene glycols as aqueous moieties. The probe is utilized to image exogenous and endogenous CO under different stimulated conditions in live cells.
Subject(s)
Boron Compounds , Carbon Monoxide , Palladium , Fluorescent Dyes , Spectroscopy, Near-InfraredABSTRACT
KYKZL-1, a newly synthesized compound with COX/5-LOX dual inhibition, was subjected to the inhibitory activity test on Hep G2 growth. We found that KYKZL-1 inhibited the growth of Hep G2 cells via inducing apoptosis. Further studies showed that KYKZL-1 activated caspase-3 through cytochrome c release from mitochondria and down regulation of Bcl-2/Bax ratio and reduced the high level of COX-2 and 5-LOX. As shown in its anti-inflammatory effect, KYKZL-1 also exhibited inhibitory effect on the PGE2 and LTB4 production in Hep G2 cells. Accordingly, exogenous addition of PGE2 or LTB4 reversed the decreases in cell viability. In addition, KYKZL-1 caused cell cycle arrest at the S-G2 checkpoint via the activation of p21(CIP1) protein and down-regulation of cyclin A expression. These data indicate that the growth inhibitory effect of KYKZL-1 is associated with inhibition of AA metabolites and caspase-3 pathway and cell cycle arrest. Combined with our previous findings, KYKZL-1 exhibiting COX/5-LOX inhibition may be a promising potential agent not only for inflammation control but also for cancer prevention/therapy with an enhanced gastric safety profile.
Subject(s)
Arachidonic Acid/antagonists & inhibitors , Caspase 3/metabolism , Cell Cycle Checkpoints/drug effects , Growth Inhibitors/pharmacology , Phenylpropionates/pharmacology , Signal Transduction/drug effects , Stilbenes/pharmacology , Arachidonic Acid/metabolism , Cell Cycle Checkpoints/physiology , Cell Survival/drug effects , Cell Survival/physiology , Enzyme Activation/drug effects , Enzyme Activation/physiology , Hep G2 Cells , Humans , Signal Transduction/physiologyABSTRACT
A luminosilicate glass samples doped with Eu203 and Dy2O3 were prepared under the air and reducing atmosphere respectively. The excitation spectra, emission spectra and thermal luminescence spectra of the samples prepared under different atmosphere were measured. Luminescence mechanism of the afterglow glasses wa s analyzed. The results showed that the sample prepared under an ambient atmosphere has no afterglow. Its excitation and emission spectra showed the typical transitions of (5)Di( i = 0, 1) --> (7)Fj (j = 0-4) from Eu3+ but the sample reheated at a reducing atmosphere has afterglow. The sample doped with Eu2+ shows luminescence peaking at 462 nm. And the sample codoped with Eu2+ and Dy3+ shows luminescence peaking at 457 nm. The afterglowluminescence can last more than 12 h.
