A narrative review of research linking non-sexual social touch to sleep quality.

This narrative review describes the current state of the literature that has examined associations between non-sexual social touch (i.e., affectionate touch, touch therapies, touch with animals and inanimate objects that mimic social touch) and sleep quality. It also highlights areas for future research to clarify the links and to identify underlying mechanisms. Most existing studies have focussed on and shown positive effects of touch therapies (e.g., massage, therapeutic touch) on sleep quality in clinical populations. Although there are fewer studies examining how other forms of social touch are linked with sleep quality, the existing research provides preliminary evidence supporting affectionate touch (e.g., hugging, skin-to-skin contact) and tactile contact with animals (e.g., dogs) and objects that mimic social touch (e.g., robots, weighted blankets) as predictors of better sleep quality, while touch deprivation and touch aversion are associated with worse sleep quality. Informed by the existing literature, we additionally reviewed potential relational-cognitive (e.g., felt-security) and neurobiological (e.g., oxytocin) mechanisms likely to underlie associations between social touch and sleep quality. Overall, current research supports associations between non-sexual social touch and sleep quality. However, future research is needed to establish these links for specific forms of social touch (and in various populations), to test explanatory mechanisms, and to identify boundary conditions. Understanding associations between non-sexual social touch and sleep quality can inform the development of touch-based interventions to improve sleep quality and health.

Cyclical Links Between Daily Partner Interactions and Sleep Quality in Older Adult Couples: The Mediating Role of Perceived Partner Responsiveness and Negative Affect.

Researchers have found significant associations between romantic relationship experiences and sleep quality. However, most existing studies are cross-sectional, few have focused on the aging population, and few have considered mechanisms underlying such associations. To address these gaps, 238 older adult couples completed 7-day daily diaries, reporting on their daily relationship, emotional, and sleep experiences. Multilevel structural equation modeling revealed that husbands' higher negative partner interactions and lower positive partner interactions were indirectly associated with their own and their wives' decreased sleep quality that night via lower perceived partner responsiveness and increased negative affect. Moreover, decreased sleep quality was associated with participants' and their partners' increased negative partner interactions and participants' decreased positive partner interactions the next day, with no significant gender differences. This research provides a foundation for future research on cyclical associations between romantic relationship experiences and sleep quality, with implications for relationship-based interventions to improve sleep quality.

Reticular Chemistry of the Fcu-Type Gd(III)-Doped Metal-Organic Framework for T1 -Weighted Magnetic Resonance Imaging.

Nanoscale metal-organic frameworks (nanoMOFs) are emerging as an important class of nanomaterials for the systematical investigation of biomedically relevant structure-property relationship (SPR) due to their highly tailorable features. In this work, the reticular chemistry approach is shown to explore the SPR of a fcu-type Zr(IV)-nanoMOF for T1 -weighted magnetic resonance imaging (MRI). Isoreticular replacement of the eight-coordinated square-antiprismatic Zr(IV) by nine-coordinated Gd(III) brings a stoichiometric water capped on the square-antiprismatic site, enabling the relaxation transfer in the inner-sphere, giving the r1 value of 4.55 mM-1 ·s-1 at the doping ratio of Gd : Zr = 1 : 1. Then, these isoreticular engineering studies provide feasible ways to facilitate the relaxation transfer in the second- and outer-sphere of the Gd(III)-doped Zr-oxo cluster for the relaxation respectively. Finally, these in vitro and in vivo MRI studies revealed that the Gd(III)-doped Zr-oxo cluster aggregated underlying the fcu-type framework surpasses its discrete molecular cluster for MRI. These results demonstrated that there is plenty of room inside MOFs for T1 -weighted MRI by reticular chemistry.

The 3D Controllable Fabrication of Nanomaterials with FIB-SEM Synchronization Technology.

Nanomaterials with unique structures and functions have been widely used in the fields of microelectronics, biology, medicine, and aerospace, etc. With advantages of high resolution and multi functions (e.g., milling, deposition, and implantation), focused ion beam (FIB) technology has been widely developed due to urgent demands for the 3D fabrication of nanomaterials in recent years. In this paper, FIB technology is illustrated in detail, including ion optical systems, operating modes, and combining equipment with other systems. Together with the in situ and real-time monitoring of scanning electron microscopy (SEM) imaging, a FIB-SEM synchronization system achieved 3D controllable fabrication from conductive to semiconductive and insulative nanomaterials. The controllable FIB-SEM processing of conductive nanomaterials with a high precision is studied, especially for the FIB-induced deposition (FIBID) 3D nano-patterning and nano-origami. As for semiconductive nanomaterials, the realization of high resolution and controllability is focused on nano-origami and 3D milling with a high aspect ratio. The parameters of FIB-SEM and its working modes are analyzed and optimized to achieve the high aspect ratio fabrication and 3D reconstruction of insulative nanomaterials. Furthermore, the current challenges and future outlooks are prospected for the 3D controllable processing of flexible insulative materials with high resolution.

Mechanisms Linking Attachment Orientation to Sleep Quality in Married Couples.

There is emerging evidence for links between relationship factors and sleep quality. Existing research linking attachment orientation to sleep quality has yielded inconsistent effects, has focused on younger samples, and has not considered underlying mechanisms of action. This research addressed these gaps in two studies that investigated the links between attachment orientation and sleep quality in both younger/middle-aged (Study 1) and older (Study 2) adult couples using Actor-Partner Interdependence Models. We also tested mediating effects of relationship-specific security and negative affect. In both studies, participants completed surveys assessing their attachment orientation, sleep quality, and the proposed mediators. Both studies revealed that relationship-specific security and negative affect mediated the negative association between insecure attachment and one's own sleep quality. This research enhances our understanding of how attachment orientation affects sleep quality, provides a foundation for future research on relationship influences on sleep, and suggests avenues for improving sleep quality.

Boundary delineation and grading functional zoning of Sanjiangyuan National Park based on biodiversity importance evaluations.

Recently, China advanced a policy of establishing a protected area system with national parks as the main body, and so the development of protected areas has entered a transitional period. However, to promote the coordinated development of economic construction and ecological protection of protected areas, their management needs to be more comprehensive and refined. Therefore, a more quantitative and refined spatial planning and management method for protected areas is urgently needed. This study took Sanjiangyuan National Park as the research object and considered the three biodiversity elements of species, ecosystems and landscape. The maximum entropy (MaxEnt) model, Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST) model, and Fragstats software were used to determine the areas with highly suitable species habitat, areas of high ecosystem service value and areas of high landscape diversity. Based on these areas, Marxan software was used to calculate the irreplaceability value, identify the boundary of Sanjiangyuan National Park and clarify the gaps of the existing Sanjiangyuan National Park in the space. We suggest that at least 18,569 km2 of the eastern part of the Sanjiangyuan Region with a high irreplaceability value should be included in the existing Sanjiangyuan National Park. Moreover, the first-level zones were also classified based on irreplaceability, and the second-level functional zones were classified by K-means clustering based on the evaluation of ecological vulnerability and economic construction suitability. The first-level zones include "core conservation zones" and "general control zones", which had areas of 71,758.20 km2 (57.99%) and 51,980.13 km2 (42.01%), respectively. The core conservation zones were further zoned into primary vulnerable zones and secondary vulnerable zones. The subzones of the general control zones are multifunctional and they are tourism-grazing-living zones, grazing-living zones, tourism-living zones, tourism zones and other areas.

An integrated assessment system for the carrying capacity of the water environment based on system dynamics.

Implementing an integrated assessment system for the carrying capacity of water environments should include recognizing and eliminating warning signs based on future predictions. However, existing methodologies tend to ignore the warning methods already in place, and current studies fail to quantify water ecology issues adequately. To help solve these problems, the ecological footprint that involves water uses a procedural indicator system, which adopts an early warning methodology system approach. This reconstruction has devised definitions, recognizes hazards, states forecasts status, analyses signs, judges situations, distinguishes levels, and eliminates risks. Based on these procedures, a dynamic system model has been developed, comprising five subsystems with an overarching parent system. These subsystems are population, ecology, water resources, water environment, and water ecology. The simulation involves carrying rates for the water environment, water resource, water ecology, and the level of harmony between society and the environment. All these serve to describe the water environment carrying capacity, i.e., the upper limit of the capacity to supply resources, remove pollutants, and offer sustainable ecological services. To properly quantify the carrying capacity, the water environment carrying rate was assessed by a comprehensive analysis of the water environment, water resources, and water ecological carrying rate. The carrying rates were calculated as the ratios of currently existing pressure to the maximum pressure that can be born. When values are greater than 1, they indicate overload because the actual pressure is greater than the pressure they can bear. The degree of coordination between economy and environment was standardized to range between 0 and 1. The larger the value, the more harmonious the relationship. For this research, the North Canal basin, a basin consisting of several rivers flowing through Beijing, Tianjin, and Hebei in northern China and its surrounding areas, was chosen. The results showed that water environment and resource carrying rates would decline to 2.60 and 0.94, respectively, while the water ecology carrying rate would remain high at 10.98 by the year 2025. In addition, the degree of coordination would increase from 0.65 to 0.79. These statistics mean that the overload statuses will be high for a long time, although they are expected to ease gradually. Besides, the relationship between society and the environment would become more stable. Considering both the overload statuses and the relationship between society and the environment, the warning signs would not vanish. Based on predictions, the measures used were explained from three perspectives, i.e., alleviating pressures, enhancing carrying capacities, and finding a balance between society and the environment. Finally, the effects of the measures were estimated quantitatively.

Spatial-temporal variation, sources and driving factors of organic carbon burial in rift lakes on Yunnan-Guizhou plateau since 1850.

Burial of organic carbon (OC) in rift lakes on plateau is an important part of the global cycle. It is affected by natural and anthropogenic factors. In this study, we selected the sediment records of 7 rift lakes on the Yunnan-Guizhou Plateau to study spatial-temporal variation, sources and driving factors of organic carbon burial since 1850. The analysis of the temporal and spatial trend of carbon burial shows that the TOC concentration, TOC flux, C: N and mass accumulate rate have increased significantly since 1850. Co-occurrence network analysis indicated that a strong correlation between the TOC concentration and silty. TOC concentration were identified as core genera due to their high concentration. Carbon isotope tracing results show that before 1950, endogenous OC input played a dominant role, and after 1950, the proportion of exogenous OC increased. Canonical correlation analysis indicated that after 1950, agriculture intensification and population increase become one of the factors affecting the carbon burial of lakes in this area. The result of this study indicate that anthropogenic factors have become the main factors promoting carbon burial in rift lakes on the plateau.

Ecological network analysis of an urban water metabolic system: Integrated metabolic processes of physical and virtual water.

The contradiction between social economy and water environment has become increasingly prominent, and the analysis of urban water metabolism system (UWMS) represents a problem-solving approach from the perspective of the entire flow process. However, a comprehensive UWMS model that considers both physical and virtual water flows is currently lacking. This paper presents an innovative application of an ecological network model of the UWMS-integrated metabolic process of physical and virtual water in Xining during the 2002-2018 period. By analysing and screening the metabolic characteristics, metabolic structure and metabolic relationships, the sustainability of the UWMS is evaluated in depth, and the main causes and critical compartments of the unhealthy metabolic process are identified. The findings show that the UWMS in Xining maintains a moderate level of robustness (the average R = 0.48) with limited metabolic efficiency. Since 2012, the water management policies in Xining have been significantly strengthened, contributing to a slight increase in robustness by improving the water use efficiency and metabolic structure. The integrated metabolic process is unhealthy because the metabolic structure is not reasonable, and the systematic metabolic relationship tends to be antagonistic due to the network mutualism index dropping to 1.0 during 2016-2018. We conclude that efficient irrigation management, more infrastructure projects for the sewer network, wastewater treatment and recycling could provide effective support to enhance the coordinate development of the social economy and water environment in Xining.

Fabrication and characterization of anchovy protein hydrolysates-polyphenol conjugates with stabilizing effects on fish oil emulsion.

Conjugation between peptides and polyphenols could improve their bioactive and functional properties. The improvement effects of anchovy protein hydrolysates (APH) -polyphenol (catechin (CA), gallic acid (GA), tannic acid (TA)) conjugates were investigated. The content of protein and polyphenols and ratio of polyphenols/peptides in conjugates increased as the number of OH group increased with TA > CA > GA. Results showed that APH-CA and APH-GA exhibited the highest ORAC and ABTS+ scavenging capacity, respectively. Mass spectrometry analysis suggested the highest number of bioactive peptides were identified in APH-CA 5:1 (APH/polyphenols). The physical stability of fish oil emulsions during storage was significantly enhanced by TA 5:1 conjugate followed by CA 5:1 conjugate. The oxidative stability was remarkably elevated by APH-GA 10:1. This was due to the antioxidant capacity and the peptides adsorbed at the interfacial. This study demonstrated that APH-polyphenol conjugates could bring the possibility of utilizing peptides-polyphenols in the nutraceutical and functional food ingredient fields.

No flow meter method for measuring radon exhalation from the medium surface with a ventilation chamber.

The method for measuring radon exhalation rate from the medium surface with a ventilation chamber is un-replaceable when surveying the radon exhalation rate continuously. Generally, the pump flow rate is an important parameter to obtain the radon exhalation rate from the measurement model. Our previous research indicated that the results of those measurements are inaccurate when the air change rate is not far larger than the effective decay constant. A no flow meter method is proposed for measuring radon exhalation from the medium surface with a ventilation chamber. A constant K is used to replace the sum of the air change rate and the effective decay constant. The radon exhalation rate and the value of K can be obtained by nonlinear data fitting through a novel model. The air flow rate is not a parameter of this model, and the flow meter is unnecessary in this measurement. The radon exhalation rates obtained by verification experiments are within the accepted values for the reference value. This method can be applied to develop and improve the instruments for measuring radon exhalation rate.

Exploring the influencing paths of natives' conservation behavior and policy incentives in protected areas: Evidence from China.

To enhance ecosystem conservation in protected areas, natives' behavior, mainly the use of natural resources or direct human disturbance to ecosystems, must be well intervened and regulated. To explore the determinants and incentives of the conservation behavior of natives, this paper establishes a theoretical behavior model emphasizing livelihood assets and conservation policy impacts, and specifies it with structural equation modeling. The protected areas of Chishui county in southwest China and the Three-River-Source National Park in the Qinghai-Tibet Plateau were selected for empirical analyses based on household surveys. Results show that conservation intentions that shape the natives' conservation behavior are collectively decided by conservation attitude, subjective norm, and perceived behavioral control. Livelihood assets exert indirect positive effects on conservation behavior by influencing the aforementioned psychological variables. The impacts on conservation behavior of the governmental policy instruments, Grain to Green Program, Grassland Ecological Protection Subsidy and Incentive Policy, and Ecological Ranger Post Setting are evaluated. The command & control functions of the first two instruments are verified to be effective. The propaganda & education functions of all three policy instruments affect conservation intention and behavior by influencing conservation attitude, subjective norm, and perceived behavioral control of the natives. The ecological compensation functions of the policies prevent the loss of the natives' livelihood assets but fail to enhance it. The findings demonstrate that besides the direct impacts on conservation behavior of the protection policy instruments, the indirect policy impacts affecting the protection consciousness of natives should not be underestimated. In the long run, a suggestion is to strengthen eco-compensation to improve livelihood assets, to better promote the protection behavior of the local residents and obtain sustainable ecosystem conservation in the protected areas. This research demonstrates applicability and feasibility of the behavioral theory and causal model method in natural conservation study and shows strong policy implication.

Identification of conflict between wildlife living spaces and human activity spaces and adjustments in/around protected areas under climate change：A case study in the Three-River Source Region.

Overlap between wildlife living spaces and human activity spaces represents one of the main causes of human-wildlife conflict. It is therefore necessary to identify and adjust the spatial distribution of conflicts because climate change will modify the ranges and locations of species. The MaxEnt model was used to simulate the distribution of wildlife living spaces under current and future climatic conditions (RCP4.5 scenario) based on species distribution data and environmental data from the Three-River Source Region. Furthermore, the logistic-cellular automata model was used to simulate the changes in human activity spaces (construction land and arable land) by 2050. Areas of overlap between wildlife living spaces and human activity spaces were determined by overlay analysis. Then, the areas of construction and arable land that would conflict with wildlife were redistributed outside highly suitable wildlife living spaces. In addition, ecological corridors connecting current and future living spaces were planned for the migration of certain species in response to climate change based on the minimum cumulative resistance model. The results showed that the areas of highly suitable wildlife living spaces will decrease under the influence of climate change if global warming occurs. In the future, the area of overlap between highly suitable wildlife living spaces and construction land will be 125 km2, and that between highly suitable wildlife living spaces and arable land will be 340 km2. The redistribution of these areas will cause human activity spaces to become more concentrated. Moreover, 110 ecological corridors should be constructed across roads in the Three-River Source Region.

MicroRNA-29-3p Regulates Hepatocellular Carcinoma Progression Through NF-κB Pathway.

BACKGROUND: Hepatocellular carcinoma (HCC) is the most common primary liver cancer and accounts for over 90% of all primary liver cancers. Increasing evidence suggests that microRNAs (miRNAs) mediate signaling pathways by gene expression regulation. METHODS: In this study, we evaluated the role of miR-29a-3p in HCC progression. MiR-29a-3p was found significantly down-regulated in HCC tissues compared to adjacent non-tumor tissues. Meantime, PTEN expression was up-regulated in HCC tissues. Moreover, NF-κB activity was decreased following PTEN up-regulation. RESULTS: In vitro assays in the HCC cell line BEL7402 demonstrated that miR-29a-3p suppresses cell proliferation. CONCLUSIONS: miR-29a-3p participates in the HCC progression by regulation of NF-κB pathway via targeting PTEN.

Erinacine Facilitates the Opening of the Mitochondrial Permeability Transition Pore Through the Inhibition of the PI3K/ Akt/GSK-3ß Signaling Pathway in Human Hepatocellular Carcinoma.

BACKGROUND/AIMS: Erinacine, which is extracted from the medicinal mushroom Hericium erinaceus, is known to play anticancer roles in human cancers. The following study aims to investigate the role of erinacine in the opening of the mitochondrial permeability transition pore (MPTP) in hepatocellular carcinoma (HCC) through the PI3K/Akt/GSK-3ß signaling pathway and highlights the applicability of erinacine in HCC treatments. METHODS: HCC and paracancerous tissues were obtained from 85 HCC patients who've undergone surgical resection. Immunohistochemistry was adopted to detect positive expression of PI3K, Akt, and GSK-3ß. Treatment of HepG-2 with LY294002 (an inhibitor of the PI3K/Akt/GSK-3ß signaling pathway) and different concentration of erinacine was performed to determine the involvement of LY294002 in erinacine action. The expressions of PI3K, Akt, GSK-3ß, CyclinD1, Vimentin, ß-catenin, Bcl-2, E-cadherin, Bax, and caspase-9 were determined by RT-qPCR and Western blot analysis. Cell viability, colony formation rate, migration, invasion, cycle, and apoptosis were detected by MTT, colony formation, wound healing assay, Transwell assay, and flow cytometry, respectively. The size and weight of xenograft tumors were observed in nude mice. Mitochondrial membrane potential in HepG-2 was determined using laser scanning confocal microscopy following JC-1 staining. Mitochondrial Ca2+ indicator Rhod-2, AM was used to detect the changes of mitochondrial Ca2+, while western blot analysis was employed to detect the presence levels of cytochrome C (cyt-C). RESULTS: The results revealed that PI3K, Akt, and GSK-3ß were up-regulated in HCC tissues. Erinacine or LY294002 led to a decrease in mitochondrial membrane potential, increase in intracellular mitochondrial Ca2+, and the release of cyt-C in mitochondria. In addition, Erinacine was found to decrease the mitochondrial membrane potential, expression of PI3K, Akt, GSK-3ß, CyclinD1, Vimentin, ß-catenin, and Bcl-2, cell proliferation, colony formation ability, migration, invasion, and xenograft tumor size, while E-cadherin, Bax, and caspase-9 expression, and cell apoptosis were elevated in a dose-dependent manner. Erinacine also stimulated the effects of LY294002 on the HCC. Following the addition of 500 µM Erinacine and MPTP opening inhibitor CsA, we found that the mitochondrial membrane potential level increased, while mitochondrial Ca2+ and Cyt-C decreased from the mitochondria. CONCLUSION: The results from the study demonstrated that erinacine induced MPTP opening, facilitates the release of cyt-C, and inhibited cell proliferation, migration, and invasion, while it promotes apoptosis by inactivating the PI3K/Akt/GSK-3ß signaling pathway, preventing the progression of HCC.

Let-7b contributes to hepatocellular cancer progression through Wnt/ß-catenin signaling.

Elevated evidences show that microRNAs (miRNAs) play vital roles in tumor progression regulation. However, the functional role of let-7b in hepatocellular carcinoma (HCC) is still largely unknown. In this study, we try to investigate the biological activity of let-7b in human HCC cells and try to find the potential regulatory signaling pathway. Our results indicate that let- 7b was remarkably down-regulated in human HCC tissues by qRT-PCR. In addition, let-7b overexpression decreased the expression of ß-catenin and c-Myc, while upregulated E-cadherin expression in HCC cells which was verified by quantitative real-time PCR (qRT-PCR) and western blotting. Furthermore, Wnt/ß-catenin was involved in let-7b biological activity which was revealed by luciferase assay. Moreover, Wnt/ß-catenin signaling inhibitor blocks HCC cell proliferation which is as the same pattern as let-7b overexpression inhibits in HCC cells proliferation. In conclusion, down-regulated let-7b promotes HCC cell proliferation through Wnt/ß-catenin signaling in HCC cells. These results suggested that appropriate manipulation of let-7b might be a new treatment of human HCC in the future.

Tauroursodeoxycholic acid inhibits endoplasmic reticulum stress, blocks mitochondrial permeability transition pore opening, and suppresses reperfusion injury through GSK-3ß in cardiac H9c2 cells.

This study investigates whether inhibition of endoplasmic reticulum (ER) stress prevents opening of the mitochondrial permeability transition pore (mPTP) and evaluates the corresponding signaling pathways involved in this process. Exposure of cardiac H9c2 cells to 800 µM H2O2 for 20 min opened mPTP in response to oxidative stress, as demonstrated by quenching of tetramethylrhodamine ethyl ester (TMRE) fluorescence. Oxidative stress-induced mPTP opening was rescued by the ER stress inhibitor tauroursodeoxycholic acid (TUDCA) in a dose-dependent manner at low concentrations. The PI3K and PKG inhibitors LY294002 and KT5823 inhibited the effect of TUDCA on mPTP opening, suggesting the involvement of PI3K/Akt and PKG signaling pathways. TUDCA significantly increased glycogen synthase kinase 3 (GSK-3ß) phosphorylation at Ser-9, with peak effect at 30 µM TUDCA. The level of GRP78 (ER chaperone) expression was significantly upregulated by 30 µM TUDCA. TUDCA-induced increases in Akt and GSK-3ß phosphorylation were inhibited by LY294002, whereas KT5823 suppressed TUDCA-induced increases in VASP and GSK-3ß phosphorylation. Oxidative stress severely affected cell morphology and ultrastructure. TUDCA prevented H2O2-induced ER swelling and mitochondrial damage. TUDCA boosted the viability of cells disrupted by ischemia/reperfusion (I/R), indicating that TUDCA eased reperfusion injury. However, TUDCA did not improve the viability of cells expressing the constitutively active GSK-3ß mutant (GSK-3ß-S9A-HA) that were subjected to I/R, suggesting an essential role of GSK-3ß inactivation in TUDCA-mediated cardioprotection against reperfusion damage. These data indicate that ER stress inhibition prevents mPTP opening and attenuates reperfusion injury through GSK-3ß inactivation. The PI3K/Akt and PKG pathways may mediate GSK-3ß inactivation.

Effects of cytomegalovirus infection on the prognosis of inflammatory bowel disease patients.

The aim of the present study was to investigate the effects of cytomegalovirus (CMV) infection on the prognosis of inflammatory bowel disease (IBD). Various databases were searched using a combination of keywords associated with CMV infection and IBD. Subsequent to the selection of relevant studies in line with strict inclusion and exclusion criteria, a meta-analysis was conducted using the Stata 12.0 software. A total of 195 studies were initially retrieved, including 28 studies in Chinese and 167 in English. Following the exclusion of unsuitable studies, 7 cohort studies with 374 IBD patients were included in the meta-analysis. The results of the present study identified significant differences between patients with and without CMV infection regarding the disease duration of IBD [standardized mean difference, -0.81; 95% confidence interval (CI), -1.19 to -0.43; P<0.001], the efficacy of corticosteroid therapy [relative risk (RR), 1.24; 95% CI, 1.02-1.49; P=0.029], the colectomy rate (RR, 2.13; 95% CI, 1.03-4.40; P=0.042) and the incidence of severe IBD (RR, 1.32; 95% CI, 1.04-1.67; P=0.022). Considering the IBD onset area, patients with CMV infection may have higher susceptibility to pancolitis (RR, 1.31; 95% CI; 1.01-1.72; P=0.045); however, no difference in susceptibility to left-sided IBD was observed between patients with or without CMV infection (RR, 0.97; 95% CI, 0.72-1.30; P=0.828). In conclusion, CMV infection may be associated with the disease duration, efficacy of corticosteroid therapy, colectomy rate, severe IBD incidence and disease location of IBD; thus, the presence of CMV infection may be considered as an important biomarker for determining the prognosis of IBD.

Carnosol protects against spinal cord injury through Nrf-2 upregulation.

BACKGROUND: Carnosol is an ortho-diphenolic diterpene with excellent antioxidant potential. The present study was designed to identify the protective role of carnosol against spinal cord injury (SCI)-induced oxidative stress and inflammation in Wistar rats. METHODS: In the present study, oxidative stress status was determined through estimating total antioxidant capacity, total oxidant status, lipid peroxide content, protein carbonyl and sulfhydryl levels, reactive oxygen species (ROS), antioxidant status (superoxide-dismutase, catalase, glutathione, glutathione peroxidase, glutathione-S-transferase). Inflammatory effects were determined by analyzing the expression of NF-κB and COX-2 through Western blot analysis. Further, carnosol-mediated redox homeostasis was analyzed by determining p-AKT and Nrf-2 levels. RESULTS: SCI resulted in a significant increase in oxidative stress status through increased ROS generation, total oxidant levels, lipid peroxide content, protein carbonyl and sulfhydryl levels. The antioxidant status in SCI rats was significantly reduced, indicating imbalance in redox status. In addition, the expression of NF-κB and COX-2 was significantly upregulated, while p-AKT and Nrf-2 levels were downregulated in SCI rats. However, treatment with carnosol showed a significant enhancement in the antioxidant status with concomitant decline in oxidative stress parameters. Further, carnosol treatment regulated the key proteins in inflammation and redox status through significant downregulation of NF-κB and COX-2 levels and upregulation of p-AKT and Nrf-2 expression. CONCLUSION: Thus, the present study shows for the first time on the protective role of carnosol against SCI-induced oxidative stress and inflammation through modulating NF-κB, COX-2 and Nrf-2 levels in Wistar rats.