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1.
Nanotechnology ; 35(38)2024 Jul 04.
Article in English | MEDLINE | ID: mdl-38925105

ABSTRACT

Lu doped Hf0.5Zr0.5O2(HZO) ferroelectric films were prepared on Pt/TiN/SiO2/Si substrate by chemical solution deposition method, and an interfacial engineering strategy for improving the ferroelectric property was explored by capping the Lu doped HZO films with a cerium oxide layer. Compared with the Lu doped HZO film without the CeOxcoating layer, the Lu doped HZO film with the CeOxcoating layer has a larger remanent polarization (2Pr= 34.72µC cm-2) and presents weaker wake-up behavior, which result from the higher orthogonal phase ratio and the lower oxygen vacancy of the CeOxcoated Lu doped HZO film. In addition, the CeOxcoating can remarkably improve the fatigue resistance and retention performance of the Lu doped HZO films. It is hoped that the results can provide an effective approach for the realization of high-performance and highly reliable hafnium oxide based ferroelectric thin films.

2.
Chemosphere ; 362: 142687, 2024 Jun 25.
Article in English | MEDLINE | ID: mdl-38936488

ABSTRACT

Effective dewatering is vital for both sludge treatment and resource recovery. This study focuses on converting post-anaerobic digested sludge into biochar to enhance sludge dewatering. The sludge-derived biochar is further modified with polyacrylamide (PAM-ADBC) and applied with sulfuric acid-modified montmorillonite (HMTS) for better performance. Significant advancements in dewatering were noted, even at reduced HMTS (0.1 g/g DS) and PAM-ADBC (25 g/kg DS) dosages. These improvements resulted in a remarkable 41.96% enhancement in capillary suction time (17.2 s) and a notable 20.26% reduction in moisture content (66.33%), respectively, all while maintaining a stable pH level. HMTS, with leached cations, improved dewatering by decomposing the extracellular polymeric substance structure through electro-neutralization to release the internal bound water within sludge flocs. Simultaneously, PAM-ADBC coagulated decomposed sludge particles into larger flocs to form a skeletal structure with itself to discharge internal water in compression dewatering. This study introduces a resource recovery method for anaerobically digested sludge and highlights its potential for sustainable utilization.

3.
Biophys J ; 2024 Jun 21.
Article in English | MEDLINE | ID: mdl-38909279

ABSTRACT

Isolated bovine adrenal chromaffin cells exposed to single 2-, 4-, or 5-ns pulses undergo a rapid, transient rise in intracellular Ca2+ mediated by Ca2+ entry via voltage-gated Ca2+ channels (VGCCs), mimicking the activation of these cells in vivo by acetylcholine. However, pulse durations 150 ns or longer elicit larger amplitude and longer-lived Ca2+ responses due to Ca2+ influx via both VGCCs and a yet to be identified plasma membrane pathway(s). To further our understanding of the differential effects of ultrashort versus longer pulse durations on Ca2+ influx, chromaffin cells were loaded with calcium green-1 and exposed to single 3-, 5-, 11-, 25-, or 50-ns pulses applied at their respective Ca2+ activation threshold electric fields. Increasing pulse duration from 3 or 5 ns to only 11 ns was sufficient to elicit increased amplitude and longer-lived Ca2+ responses in the majority of cells, a trend that continued as pulse duration increased to 50 ns. The amplification of Ca2+ responses was not the result of Ca2+ release from intracellular stores and was accompanied by a decreased effectiveness of VGCC inhibitors to block the responses and a reduced reliance on extracellular Na+ and membrane depolarization to evoke the responses. Inhibitors of pannexin channels, P2X receptors, or non-selective cation channels failed to attenuate 50-ns-elicited Ca2+ responses, ruling out these Ca2+-permeable channels as secondary Ca2+ entry pathways. Analytical calculations and numerical modeling suggest that the parameter that best determines the response of chromaffin cells to increasing pulse durations is the time the membrane charges to its peak voltage. These results highlight the pronounced sensitivity of a neuroendocrine cell to pulse durations differing by only tens of nanoseconds, which has important implications for the future development of nanosecond pulse technologies enabling electrostimulation applications for spatially focused and graded in vivo neuromodulation.

4.
Chemosphere ; 355: 141824, 2024 May.
Article in English | MEDLINE | ID: mdl-38548082

ABSTRACT

The complexity and high cost to separate and recover short chain fatty acids (SCFAs), ammonium ions, and phosphates in the sludge fermentation liquid hinder the application of sludge anaerobic fermentation. In this study, an interesting phenomenon was found in a sludge anaerobic fermenter with a dynamic membrane (DM) which could not only enhance SCFAs production but also retain most SCFAs in fermenter. The separation factor of DM for NH3-N/SCFAs and PO43-/SCFAs throughout the DM development were about 40 and 80, respectively. Analysis reveals that rejection of SCFAs by DM could not be simply correlated to molecular weight or membrane pore size. The rejection mechanisms might be dominated by Donnan rejection. In addition, biodegradation in the DM may also have contribution. Findings of this study suggest the potential of DM as an economical technology for nutrients and SCFAs recover.


Subject(s)
Bioreactors , Sewage , Anaerobiosis , Fermentation , Nutrients , Fatty Acids, Volatile , Hydrogen-Ion Concentration
5.
Sci Total Environ ; 916: 170232, 2024 Mar 15.
Article in English | MEDLINE | ID: mdl-38278257

ABSTRACT

Anaerobic fermentation is an effective method to harvest volatile fatty acids (VFAs) from waste activated sludge (WAS). Accurately predicting and optimizing VFAs production is crucial for anaerobic fermentation engineering. In this study, we developed machine learning models using two innovative strategies to precisely predict the daily yield of VFAs in a laboratory anaerobic fermenter. Strategy-1 focuses on model interpretability to comprehend the influence of variables of interest on VFAs production, while Strategy-2 takes into account the cost of variable acquisition, making it more suitable for practical applications in prediction and optimization. The results showed that Support Vector Regression emerged as the most effective model in this study, with testing R2 values of 0.949 and 0.939 for the two strategies, respectively. We conducted feature importance analysis to identify the critical factors that influence VFAs production. Detailed explanations were provided using partial dependence plots and Shepley Additive Explanations analyses. To optimize VFAs production, we integrated the developed model with optimization algorithms, resulting in a maximum yield of 2997.282 mg/L. This value was 45.2 % higher than the average VFAs level in the operated fermenter. Our study offers valuable insights for predicting and optimizing VFAs production in sludge anaerobic fermentation, and it facilitates engineering practice in VFAs harvesting from WAS.


Subject(s)
Fatty Acids, Volatile , Sewage , Fermentation , Anaerobiosis , Hydrogen-Ion Concentration
6.
Hepatology ; 79(1): 167-182, 2024 Jan 01.
Article in English | MEDLINE | ID: mdl-37368993

ABSTRACT

BACKGROUND AND AIMS: Chronic hepatitis B (CHB) is caused by HBV infection and affects the lives of millions of people worldwide by causing liver inflammation, cirrhosis, and liver cancer. Interferon-alpha (IFN-α) therapy is a conventional immunotherapy that has been widely used in CHB treatment and achieved promising therapeutic outcomes by activating viral sensors and interferon-stimulated genes (ISGs) suppressed by HBV. However, the longitudinal landscape of immune cells of CHB patients and the effect of IFN-α on the immune system are not fully understood. APPROACH AND RESULTS: Here, we applied single-cell RNA sequencing (scRNA-seq) to delineate the transcriptomic landscape of peripheral immune cells in CHB patients before and after PegIFN-α therapy. Notably, we identified three CHB-specific cell subsets, pro-inflammatory (Pro-infla) CD14+ monocytes, Pro-infla CD16+ monocytes and IFNG+ CX3CR1- NK cells, which highly expressed proinflammatory genes and positively correlated with HBsAg. Furthermore, PegIFN-α treatment attenuated percentages of hyperactivated monocytes, increased ratios of long-lived naive/memory T cells and enhanced effector T cell cytotoxicity. Finally, PegIFN-α treatment switched the transcriptional profiles of entire immune cells from TNF-driven to IFN-α-driven pattern and enhanced innate antiviral response, including virus sensing and antigen presentation. CONCLUSIONS: Collectively, our study expands the understanding of the pathological characteristics of CHB and the immunoregulatory roles of PegIFN-α, which provides a new powerful reference for the clinical diagnosis and treatment of CHB.


Subject(s)
Hepatitis B, Chronic , Humans , Antiviral Agents , Interferon-alpha , Transcriptome , Sequence Analysis, RNA , Hepatitis B virus , Hepatitis B Surface Antigens , Hepatitis B e Antigens , DNA, Viral
7.
J Cell Mol Med ; 28(1): e18004, 2024 01.
Article in English | MEDLINE | ID: mdl-37864300

ABSTRACT

Nonsyndromic hearing loss (NSHL) is a genetically diverse, highly heterogeneous condition characterised by deafness, and Gasdermin E (GSDME) variants have been identified as directly inducing autosomal dominant NSHL. While many NSHL cases associated with GSDME involve the skipping of exon 8, there is another, less understood pathogenic insertion variant specifically found in Chinese pedigrees that causes deafness, known as autosomal dominant 5 (DFNA5) hearing loss. In this study, we recruited a large Chinese pedigree, conducted whole-exome and Sanger sequencing to serve as a comprehensive clinical examination, and extracted genomic DNA samples for co-segregation analysis of the members. Conservation and expression analyses for GSDME were also conducted. Our clinical examinations revealed an autosomal dominant phenotype of hearing loss in the family. Genetic analysis identified a novel insertion variant in GSDME exon 8 (GSDME: NM_004403.3: c.1113_1114insGGGGTGCAGCTTACAGGGTGGGTGT: p. P372fs*36). This variant is segregated with the deafness phenotype of this pedigree. The GSDME gene was highly conserved in the different species we analysed, and its mRNA expression was ubiquitously low in different human tissues. In conclusion, we have successfully identified a novel pathogenic insertion variant of GSDME in a Chinese pedigree that causes deafness, shedding light on the genetic basis of hearing loss within this specific family. Our findings expand the spectrum of known variants associated with GSDME-related deafness and may further support both the underlying gain-of-function mechanism and functional associations of GSDME hearing loss variants.


Subject(s)
Deafness , Hearing Loss, Sensorineural , Hearing Loss , Humans , Pedigree , Hearing Loss/genetics , Deafness/genetics , China , Mutation , Hearing Loss, Sensorineural/genetics
8.
Heliyon ; 9(12): e23005, 2023 Dec.
Article in English | MEDLINE | ID: mdl-38125507

ABSTRACT

The Tibetan people are ancient and populous, constituting the seventh-largest of the fifty-five ethnic minority groups in China. The Ngawa Tibetan and Qiang Autonomous Prefecture (NTQAP), situated on the border of northwest and southwest China, has its distinct group relationships. Short tandem repeat (STR) is extremely polymorphic and extensively used in the application of forensic medicine and population genetics. However, it is not clear the genetic information including linkage disequilibrium (LD) by 36 autosomal STR (A-STR) markers in the Tibetan group from NTQAP. The Tibetan population from NTQAP of southwest China was examined for 36 A-STR loci in the research. Every marker across the 36 A-STR loci was consistent with Hardy-Weinberg equilibrium (HWE). The results of the calculation revealed that the total discrimination power (TDP) is 1-2.2552 × 10-42 and the cumulative probability of exclusion (CPE) is 1-1.3031 × 10-16. Subsequently, a total of 345 alleles with allelic frequencies ranging from 0.00382 to 0.55343 were identified, and the allelic numbers varied from 5 in both the TH01 and TPOX markers to 28 in the SE33 locus. The Ngawa Tibetan population, along with other Chinese populations, exhibited influences from historical factors and regional distribution, as indicated by the results of population genetics analysis. We thus first explored the genetic characteristics and correlated forensic parameters of the 36 A-STR markers in NTQAP to fill the gap in the Tibetan population. It was discovered that these 36 autosomal STR markers supplemented forensic STR databases and offered extremely valuable polymorphisms for Chinese forensic applications, such as parentage testing and personal identification. Moreover, the study would contribute additional information regarding the substructure and diversity in the Chinese population.

9.
Medicine (Baltimore) ; 102(43): e34246, 2023 Oct 27.
Article in English | MEDLINE | ID: mdl-37904464

ABSTRACT

The aim of this study is to investigate the core competencies of midwives in China. Combination of qualitative research and quantitative research. A total of 100 midwives in 3 tertiary (Grade 3) hospitals in Shijiazhuang were investigated by using the Midwife Core Competency Scale, and simultaneously followed by semi-structured interviews with 12 midwives. The questionnaire survey showed that the average score of core competencies of midwives was 4.17 ± 0.17. The scores of midwives' competency for labor and delivery care (4.31 ± 0.09), new-born care (4.29 ± 0.04), and postpartum care (4.25 ± 0.13) were relatively high, while the prepregnancy care had the lowest score (3.88 ± 0.07). The interview results showed that the self-perception of core midwifery competencies was not bad, the limitations of midwives' work scope affect the core competencies, and midwifery education needs to strengthen the humanistic care and the training of obstetric knowledge and technology.


Subject(s)
Labor, Obstetric , Midwifery , Pregnancy , Female , Humans , Midwifery/education , Midwifery/methods , Qualitative Research , Hospitals , China
10.
Environ Sci Pollut Res Int ; 30(44): 99473-99483, 2023 Sep.
Article in English | MEDLINE | ID: mdl-37612553

ABSTRACT

Despite its great potential to recover energy from waste sludge, anaerobic digestion (AD) still needs to solve issues such as slow hydrolysis and H2 inhibition. This study investigated the effects of coupling microbial electrolysis cell (MEC) with AD on the CH4 yield. Results and analysis show that the CH4 yield was significantly improved in MEC-AD reactors by two factors, i.e., enhanced and accelerated hydrolysis and acidogenesis, and enrichment of hydrogenotrophic methanogens in suspended culture. Compared with graphite rod and carbon fiber brush, carbon felt (CF) as an electrode showed the best performance in terms of net energy output. The CH4 yield of MEC-AD-CF was 40.2 L CH4/kg VS, 92.3% higher than in the control group, and the VS removal rate was also increased by 47.2%. Acetoclastic methanogens were dominant in the control AD reactor, while the relative abundance of Methanobacterium, which is electroactive and known as hydrogenotrophic methanogen, increased to 24.6% in MEC-AD with CF as electrodes.


Subject(s)
Bioreactors , Sewage , Anaerobiosis , Bioreactors/microbiology , Sewage/microbiology , Methane , Electrodes
11.
J Clin Transl Hepatol ; 11(5): 1003-1010, 2023 Oct 28.
Article in English | MEDLINE | ID: mdl-37577218

ABSTRACT

Background and Aims: A functional cure, or hepatitis B virus (HBV) surface antigen (HBsAg) loss, is difficult to achieve in patients with hepatitis B virus e antigen (HBeAg)-positive chronic hepatitis B. The HBV vaccine and granulocyte-macrophage colony-stimulating factor (GM-CSF) have been reported to help reduce HBsAg levels and promote HBsAg loss. In this prospective randomized trial, we evaluated HBsAg loss in patients receiving pegylated interferon-α2b (PEGIFN-α2b) and tenofovir disoproxil fumarate (TDF), with and without GM-CSF and HBV vaccination. Methods: A total of 287 patients with HBeAg positive chronic hepatitis B and seroconversion after nucleot(s)ide analog treatment were assigned randomly to three treatment groups for 48 weeks, TDF alone (control), PEGIFN-α2b + TDF, and PEGIFN-α2b + TDF + GM-CSF + HBV vaccine. The primary endpoints were the proportions of patients with HBsAg loss and seroconversion at 48 and 72 weeks. Results: The cumulative HBsAg loss rates in the control, PEGIFN-α2b + TDF, and PEGIFN-α2b + TDF + GM-CSF + HBV vaccine groups at week 48 were 0.0%, 28.3%, and 41.1%, respectively. The cumulative HBsAg seroconversion rates in these groups at week 48 were 0.0%, 21.7%, and 33.9%, respectively. Multivariate regression analysis showed that GM-CSF use plus HBV vaccination was significantly associated with HBsAg loss (p=0.017) and seroconversion (p=0.030). Conclusions: In patients with HBeAg-positive chronic hepatitis B and seroconversion after nucleot(s)ide analog treatment, immunomodulatory/antiviral treatment regimens effectively improved HBsAg loss, and the regimen including GM-CSF and HBV vaccination was most effective.

12.
J Hazard Mater ; 459: 132073, 2023 Oct 05.
Article in English | MEDLINE | ID: mdl-37467613

ABSTRACT

The use of green, non-toxic raw materials is of great significance to the sustainable development of the environment, among which epigallocatechin gallate (EGCG) is a renewable carbon source from plants. At present, there is a lack of research on the metal-polyphenol nanomaterials their use in water decontamination. In this study, a novel SA/EGCG@Ti/SA/PVDF (SESP) sandwich membrane was prepared to effectively solve the problems of difficult recovery of nanomaterials and the leaching of metal ions. The membrane was made by scraping SA on the surface of the PVDF substrate as the bottom protective layer, depositing EGCG@Ti NPs as the functional layer, then coating SA as the surface isolation layer, and finally cross-linking with anhydrous calcium chloride. Results showed that EGCG@Ti NPs dispersed well on the surface of the SA/PVDF basement membrane. SESP sandwich membrane had good hydrothermal and acid-base stability, and it can be applied to wastewater with multiple co-existing heavy metals (e.g., Cu, Pb, Cd, and Ni). The contact angle and pure water flux of the SESP sandwich membrane with a negatively charged surface were 14.0-15.6° and 171.40 L/m2 h, respectively. The pure water flux of the regenerated membrane after BSA pollution recovered to 98.68 L/m2 h, and the interception efficiency and the interception flux of Cr(VI) were 100 % and 72.92 L/m2 h at 40 min of interception, respectively. Additionally, the removal efficiency of Cr(VI) by SESP sandwich membrane was maintained above 83 % for simulated wastewater and 100 % for actual wastewater after five adsorption-desorption cycles. Cr(VI) and Cr(III) can be removed simultaneously with the negatively charged SESP sandwich membrane. EDS and XPS analysis showed that the removal of Cr(VI) was controlled by the Donnan effect, anion exchange, chelation/complexation, and reduction mechanism. In contrast, Cr(III) was mainly influenced by electrostatic attraction and chelation/complexation mechanisms. In conclusion, the newly prepared sandwich membrane has good application potential in treating Cr(VI) wastewater.

13.
Genes (Basel) ; 14(7)2023 07 09.
Article in English | MEDLINE | ID: mdl-37510323

ABSTRACT

The Han nationality is an ancient and populous people, and different places in China may have their distinct group relationships. Luzhou area, as a crossroads of several provinces in Southwest China, lacks autosomal short tandem repeat (STR) research and population genetics research. In this study, 21 autosomal STR loci were evaluated in 1959 Han-Chinese individuals from Luzhou area. There was no substantial linkage disequilibrium (LD) among the 21 autosomal STR markers, and all markers were in Hardy-Weinberg equilibrium (HWE). The total discrimination power (TDP) and cumulative probability of exclusion (CPE) of the 21 autosomal STR loci were calculated to be 1-9.8505 × 10-16 and 1-1.9406 × 10-9, respectively. There were 333 alleles for 21 STRs with allelic frequencies ranging from 0.00026 to 0.51302, and the number of alleles ranged from 7 in locus TPOX to 29 in locus Penta E. According to the results of population comparison and population differentiation, historical influences, geographical distribution, cultural integration, and economic development may have an impact on the Luzhou Han population and other Chinese populations. These 21 STR loci were found to enrich autosomal STR information in forensic databases and provide highly informative polymorphisms for our forensic practice in China, including personal identification and parentage testing.


Subject(s)
East Asian People , Polymorphism, Genetic , Humans , Genetics, Population , Microsatellite Repeats/genetics , China
14.
Front Immunol ; 14: 1149986, 2023.
Article in English | MEDLINE | ID: mdl-37020558

ABSTRACT

SRY-box transcription factor 9 (SOX9) (OMIM 608160) is a transcription factor. The expression of SOX9 in pan-cancers and the regulation by small molecules in cancer cell lines are unclear. In the current study, we comprehensively analyzed the expression of SOX9 in normal tissues, tumor tissues and their matched healthy tissues in pan-cancers. The study examined the correlation between immunomodulators and immune cell infiltrations in normal and tumor tissues. Cordycepin (CD), an adenosine analog for SOX9 expression regulation, was also conducted on cancer cells. The results found that SOX9 protein is expressed in a variety of organs, including high expression in 13 organs and no expression in only two organs; in 44 tissues, there was high expression in 31 tissues, medium expression in four tissues, low expression in two tissues, and no expression in the other seven tissues. In pan-cancers with 33 cancer types, SOX9 expression was significantly increased in fifteen cancers, including CESC, COAD, ESCA, GBM, KIRP, LGG, LIHC, LUSC, OV, PAAD, READ, STAD, THYM, UCES, and UCS, but significantly decreased in only two cancers (SKCM and TGCT) compared with the matched healthy tissues. It suggests that SOX9 expression is upregulated in the most cancer types (15/33) as a proto-oncogene. The fact that the decrease of SOX9 expression in SKCM and the increase of SOX9 in the cell lines of melanoma inhibit tumorigenicity in both mouse and human ex vivo models demonstrates that SOX9 could also be a tumor suppressor. Further analyzing the prognostic values for SOX9 expression in cancer individuals revealed that OS is long in ACC and short in LGG, CESC, and THYM, suggesting that high SOX9 expression is positively correlated with the worst OS in LGG, CESC, and THYM, which could be used as a prognostic maker. In addition, CD inhibited both protein and mRNA expressions of SOX9 in a dose-dependent manner in 22RV1, PC3, and H1975 cells, indicating CD's anticancer roles likely via SOX9 inhibition. Moreover, SOX9 might play an important role in tumor genesis and development by participating in immune infiltration. Altogether, SOX9 could be a biomarker for diagnostics and prognostics for pan-cancers and an emerging target for the development of anticancer drugs.


Subject(s)
Deoxyadenosines , Melanoma , Humans , Animals , Mice , Adenosine , Adjuvants, Immunologic , SOX9 Transcription Factor
15.
BMC Med Genomics ; 16(1): 85, 2023 04 24.
Article in English | MEDLINE | ID: mdl-37095468

ABSTRACT

Neurofibromatosis type 1 (NF1) presents an autosomal dominant, haploinsufficient, and multisystemic disorder with patches of skin café-au-lait spots, lisch nodules in the iris, even tumors in the peripheral nervous system or fibromatous skin. In this study, a Chinese young woman who suffered from NF1 disease with first-trimester spontaneous abortion was recruited. Analysis for whole exome sequencing (WES), Sanger sequencing, short tandem repeat (STR), and co-segregation was carried out. As results, a novel, heterozygous, de novo pathogenic variant (c.4963delA:p.Thr1656Glnfs*42) of the NF1 gene in the proband was identified. This pathogenic variant of the NF1 gene produced a truncated protein that lost more than one-third of the NF1 protein at the C-terminus including half of the CRAL-TRIO lipid-binding domain and nuclear localization signal (NLS), thus leading to pathogenicity (ACMG criteria: PVS1 + PM2 + PM2). Analysis for NF1 conservation in species revealed high conservation in different species. Analysis of NF1 mRNA levels in different human tissues showed low tissue specificity, which may affect multiple organs presenting other symptoms or phenotypes. Moreover, prenatal NF1 gene diagnosis showed both alleles as wild types. Thus, this NF1 novel variant probably underlays the NF1 pathogenesis in this pedigree, which would help for the diagnosis, genetic counseling, and clinical management of this disorder.


Subject(s)
Neurofibromatosis 1 , Female , Humans , Cafe-au-Lait Spots/diagnosis , Cafe-au-Lait Spots/genetics , East Asian People , Genes, Neurofibromatosis 1 , Neurofibromatosis 1/diagnosis , Neurofibromatosis 1/genetics , Neurofibromatosis 1/pathology , Neurofibromin 1/genetics
16.
PLoS One ; 18(4): e0284381, 2023.
Article in English | MEDLINE | ID: mdl-37058539

ABSTRACT

PURPOSE: Acupuncture has been widely used in the treatment of knee osteoarthritis (KOA), but the selection of acupoints is indeterminate and lacks biological basis. The skin temperature of acupoints can reflect the state of local tissue and may be a potential factor for guiding acupoint selection. This study aims to compare the skin temperature of acupoints between KOA patients and the healthy population. STUDY DESIGN AND METHODS: This is a protocol for a cross-sectional case-control study with 170 KOA patients and 170 age- and gender-matched healthy individuals. Diagnosed patients aged 45 to 70 will be recruited in the KOA group. Participants in the healthy group will be matched with the KOA group based on mean age and gender distribution. Skin temperature of 11 acupoints (ST35, EX-LE5, GB33, GB34, EX-LE2, ST34, ST36, GB39, BL40, SP9, SP10) will be extracted from infrared thermography (IRT) images of the lower limbs. Other measurements will include demographic data (gender, age, ethnicity, education, height, weight, BMI) and disease-related data (numerical rating scale, pain sites, duration of pain, pain descriptors, pain activities). DISCUSSION: The results of this study will provide biological evidence for acupoint selection. This study is a precondition for follow-up studies, in which the value of optimized acupoint selection will be verified. TRIAL REGISTRATION: ChiCTR2200058867.


Subject(s)
Acupuncture Therapy , Osteoarthritis, Knee , Humans , Acupuncture Points , Thermography , Case-Control Studies , Cross-Sectional Studies , Osteoarthritis, Knee/therapy , Acupuncture Therapy/methods , Pain , Lower Extremity , Treatment Outcome
17.
Environ Sci Pollut Res Int ; 30(18): 54205-54222, 2023 Apr.
Article in English | MEDLINE | ID: mdl-36877394

ABSTRACT

The countries along the "Belt and Road" (B&R) have close cooperative connections economically and in trade, and these connections lead to a large amount of embodied carbon emissions and have formed a complex network of net carbon transfers. Considering 63 countries and 26 sectors, this study uses the Eora multiregional input-output (MRIO) model to construct the embodied carbon transfer networks in 1992, 1998, 2004, 2010, and 2016. Additionally, it applies the social network approach to analyze the structural characteristics and evolution of carbon flow networks in the countries and regions along the B&R. The results show that (1) from the regional perspective, the net embodied carbon flow network of trade between countries is connected with an obvious core-periphery structure. The embodied carbon transfer network generally tends to expand over time. (2) The net carbon transfer network can be divided into four blocks, among which 13 countries, such as China, India, and Russia, are part of the main spillover block, and 25 countries, such as Singapore, the UAE, and Israel, are the main beneficiary block. (3) From the sectoral perspective, the embodied carbon transfer network has generally tended to shrink. (4) The net carbon transfer network can also be divided into four blocks, with six industries, such as the wood and paper industry, constituting the main spillover block and 11 industries, such as agriculture, constituting the main beneficiary block. Our findings can provide a factual basis for the coordinated control of carbon emissions from regional and sectoral perspectives in the countries and regions along the B&R and reasonably define the responsibility of producers and consumers of embodied carbon to facilitate a more equitable and effective negotiation process for emission reductions.


Subject(s)
Carbon , Industry , Agriculture , Carbon/analysis , Carbon Dioxide/analysis , China
18.
Environ Sci Pollut Res Int ; 30(13): 39154-39168, 2023 Mar.
Article in English | MEDLINE | ID: mdl-36595173

ABSTRACT

Numerous studies have explored the adsorption of cadmium (Cd) and arsenic (As) by iron (Fe)-modified biochar, but few studies have examined in-depth the similarities and differences in the adsorption behavior of different iron types on Cd and As. In this study, sewage sludge biochar (BC) was co-pyrolyzed with self-made Fe minerals (magnetite, hematite, ferrihydrite, goethite, and schwertmannite) to treat Cd and As co-contaminated water. The adsorption of Cd and As on the Fe-modified biochar was further analyzed by adsorption kinetics, adsorption isotherms, and adsorption thermodynamics combined with a series of characterization experiments. Both SEM-EDX and XRD results confirmed the successful loading of iron minerals onto BC. Both adsorption kinetics and adsorption isotherms experiments showed that the adsorption of Cd and As by BC and the other five Fe-modified biochar was mainly controlled by chemical interactions. The results also indicated that goethite biochar (GtBC) was the most effective for the adsorption of Cd among the five Fe-modified biochar. Ferrihydrite biochar (FhBC) formed more diverse complexes, coupled with the relatively stronger electrons accepting ability, thus making it more effective for As adsorption than the others. Additionally, GtBC and hematite biochar (HmBC) were found effective for the adsorption of both Cd and As, whereas MBC was not found effective for either metal. Furthermore, combined with XPS results, the adsorption of Cd by the materials was mainly governed by Cd2+-π interactions, complexation precipitation, and co-precipitation, while oxidation reactions also existed for As.


Subject(s)
Arsenic , Water Pollutants, Chemical , Iron/analysis , Sewage , Cadmium/analysis , Minerals/chemistry , Charcoal/chemistry , Adsorption , Water Pollutants, Chemical/chemistry
19.
Mol Biol Rep ; 50(2): 1117-1123, 2023 Feb.
Article in English | MEDLINE | ID: mdl-36401065

ABSTRACT

BACKGROUND: Neurofibromatosis type 1 (NF1) is an autosomal dominant with haploinsufficient, and multisystemic disorder including patches of skin Café-au-lait spots, Lisch nodules in the iris, and tumors in the peripheral nervous systems or fibromatous skin. METHODS: Blood samples were collected and DNA was extracted from a large Chinese pedigree suffering from NF1 disease with three spontaneous abortions or death for proband. Analysis for whole exome sequencing (WES), Sanger sequencing, and co-segregation was carried out. Prenatal gene diagnosis was also carried out in amniotic fluid DNA. The expression of NF1 was conducted by bioinformatics. RESULTS: A large Chinese pedigree with NF1 was recruited and a novel, heterozygous, variant (c.4272delA: p.I1426Ffs*2) for the NF1 gene in the proband was identified. This variant of NF1 produced a truncated protein that losses half of NF1 protein at the C-terminus including the CRAL-TRIO lipid-binding domain, NLS, and a small portion of Ras-GAP domain, thus leading to pathogenicity (ACMG criteria: PVS1 + PM2). NF1 expressions in different human tissues showed low tissue specificity, which may affect multiple organs presenting different phenotypes. Moreover, prenatal gene diagnosis for NF1 showed both alleles as wild types in the fetus of the proband. CONCLUSION: We thus successfully identified a novel, pathogenic, heterozygous variant (c.4272delA:p.I1426Ffs*2) in the NF1 gene of NF1 disorder, expanding the NF1 mutation spectrum, that will help elucidate the molecular pathogenesis of NF1 disease and to contribute to the NF1 diagnosis, genetic counseling, clinical management in this large Chinese family.


Subject(s)
Neurofibromatosis 1 , Humans , Neurofibromatosis 1/genetics , Neurofibromatosis 1/diagnosis , Genes, Neurofibromatosis 1 , East Asian People , Neurofibromin 1/genetics , Cafe-au-Lait Spots/genetics
20.
Environ Sci Pollut Res Int ; 29(55): 83126-83137, 2022 Nov.
Article in English | MEDLINE | ID: mdl-35759098

ABSTRACT

Rice is the main food in China, and its pollution by heavy metals has attracted widespread attention. In this study, rice grain samples were collected from 14 prefecture-level cities in Hunan Province, China. The contents of 9 heavy metals (i.e., As, Cr, Co, Ni, Cu, Zn, Cd, Pb, and Sb) were measured using graphite digestion-inductively coupled plasma mass spectrometry (ICP-MS). Pearson correlation analysis and principal component analysis were performed to evaluate the correlation among these heavy metals. In addition, ordinary kriging interpolation were applied to investigate the spatial distribution pattern of the heavy metals. Results showed that the average concentrations of these heavy metals were 0.48 (As), 1.28 (Cr), 0.03 (Co), 0.84 (Ni), 2.39 (Cu), 15.73 (Zn), 0.28 (Cd), 0.66 (Pb), and 0.0043 (Sb) mg/kg, respectively. The single-factor pollution index (SFPI) contamination assessment showed that As, Pb, Cr, Ni, and Cd accumulated significantly in the rice grain, with over-standard rates of 100%, 100%, 64.70%, 47.05%, and 44.12%, respectively. The Sb concentrations at the sampling sites were low, and there was no obvious pollution. Health risk assessment showed that the target hazard quotient followed the order of As> Cr> Cd> Pb> 1.0> Co> Cu> Zn> Ni> Sb, and the carcinogenic risk value was in the order of Cd> Ni> As> Cr> 1.0×10-4> Pb. In particular, quick actions should be taken to regulate As, Cr, and Cd contents in rice because they posed greater non-carcinogenic and carcinogenic health risks than the others to the local residents.


Subject(s)
Metals, Heavy , Oryza , Soil Pollutants , Humans , Soil Pollutants/analysis , Cadmium/analysis , Lead/analysis , Environmental Monitoring/methods , Metals, Heavy/analysis , Risk Assessment , China , Soil/chemistry
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