Comparative study on physicochemical properties and hypoglycemic activities of intracellular and extracellular polysaccharides from submerged fermentation of Morchella esculenta.

The structural characteristic, physicochemical properties and structure-hypoglycemic activity relationship of intracellular (IPS) and extracellular (EPS) from submerged fermentation of Morchella esculenta were systematically compared and assessed. Both IPS and EPS were neutral, with a triple-helical conformation, and composed of galactose, glucose and mannose monosaccharides in different molar ratios. The molecular weight and particle size of IPS were higher than those of EPS. FTIR and SEM showed that the main functional group absorption peak intensity, glycosidic bond type and surface morphology of the two polysaccharides differed. Analysis of rheological and thermal properties revealed that the viscosity of IPS was higher than that of EPS, while thermal stability of EPS was greater than that of IPS. Hypoglycemic activity analysis in vitro showed that both IPS and EPS were non-competitive inhibitors of α-amylase and α-glucosidase. EPS showed strong digestive enzyme inhibitory activity due to its higher sulphate content and molar ratio of galactose, lower Mw and particle size. Meanwhile, with its higher Mw and apparent viscosity, IPS showed stronger glucose adsorption capacity and glucose diffusion retardation. These results indicate that IPS and EPS differed considerably in structure and physicochemical properties, which ultimately led to differences in hypoglycemic activity. These results not only suggested that IPS and EPS has the potential to be functional foods or hypoglycemic drugs, but also provided a new target for the prevention and treatment of diabetes with natural polysaccharides.

Phase-sensitive manipulations of a squeezed vacuum field in an optical parametric amplifier inside an optical cavity.

A squeezed vacuum field can be amplified or deamplified when it is injected, as the signal beam, into a phase-sensitive optical parametric amplifier (OPA) inside an optical cavity. The spectral features of the reflected quantized signal field are controlled by the relative phase between the injected squeezed vacuum field and the pump field for the OPA. The experimental results demonstrate coherent phenomena of OPA in the quantum regime and show phase-sensitive manipulations of quantum fluctuations for quantum information processing.

Electromagnetically induced transparency-like effect in the degenerate triple-resonant optical parametric amplifier.

We investigate experimentally the absorptive and dispersive properties of the triple-resonant optical parametric amplifier (OPA) for the degenerate subharmonic field. In the experiment the subharmonic field is utilized as the probe field and the harmonic wave as the pump field. We demonstrate that the electromagnetically induced transparency (EIT)-like effect can be simulated in the triple-resonant OPA when the cavity linewidth for the harmonic wave is narrower than that for the subharmonic field. However, this phenomenon cannot be observed in a double-resonant OPA. The narrow transparency window appears in the reflected field. Especially in the measured dispersive spectra of the triple-resonant OPA, a very steep variation of the dispersive profile of the subharmonic field is observed, which can result in a slow light as that observed in an atomic EIT medium.

Coherence phenomena in the phase-sensitive optical parametric amplification inside a cavity.

We theoretically and experimentally demonstrate coherence phenomena in optical parametric amplification inside a cavity. The mode splitting in the transmission spectra of a phase-sensitive optical parametric amplifier is observed. Especially, we show that a very narrow dip and peak, which are the shape of a delta function, appear in the transmission profile. The origin of the coherence phenomenon in this system is the interference between the harmonic pump field and the subharmonic seed field in cooperation with dissipation of the cavity.