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1.
Zhonghua Nei Ke Za Zhi ; 60(5): 453-458, 2021 May 01.
Article in Chinese | MEDLINE | ID: mdl-33906275

ABSTRACT

Objective: Longitudinally extensive transverse myelitis (LETM) could be seen in patients with connective tissue disease (CTD), especially systemic lupus erythematosus (SLE) or primary Sjögren's syndrome (pSS). Some patients are combined with neuromyelitis optica spectrum disorders (NMOSD)(termed CTD-LETM-NMOSD) while others without (termed CTD-LETM-non-NMOSD). The aim of this study is to compare the clinical characteristics of CTD-LETM-NMOSD patients to CTD-LETM-non-NMOSD patients. Methods: We retrospectively collected data from 40 CTD patients with LETM who were admitted to the Department of Neurology or Rheumatology at Peking Union Medical College Hospital from Jan, 2006 to Dec, 2016. They were divided into CTD-LETM-NMOSD and CTD-LETM-non-NMOSD two groups. Demographic characteristics, clinical and laboratory features were obtained from the database. Relapse rates and clinical outcome were analyzed by Kaplan-Meier method. Results: Among 40 patients with CTD, 28 (70.0%) were NMOSD while 12 (30.0%) were not. The positivity rates of anti-SSA, antibodies to aquaporin-4 (anti-AQP4) were significantly higher in patients with NMOSD than those in patients with non-NMOSD (P<0.05). Age, gender, clinical features, disease duration, anti-double-stranded DNA antibody, anti-ribosomal P antibody, antiphospholipid antibodies, expanded disability status scale (EDSS) scores, and magnetic resonance imaging (MRI) features were all comparable between two groups. CTD-NMOSD patients had significantly higher disease relapse rate (75.0% vs. 3/12, P<0.01). Conclusion: Anti-SSA and anti-AQP4 positivity is associated with NMOSD and higher relapse rates, which suggests that NMOSD in CTD-LETM patients may represent distinct characteristics and pathogenesis from patients with CTD-LETM-non NMOSD.


Subject(s)
Connective Tissue Diseases , Myelitis, Transverse , Neuromyelitis Optica , Aquaporin 4 , Autoantibodies , Connective Tissue Diseases/complications , Humans , Neoplasm Recurrence, Local , Neuromyelitis Optica/diagnostic imaging , Retrospective Studies
2.
Zhonghua Gan Zang Bing Za Zhi ; 28(7): 603-607, 2020 Jul 20.
Article in Chinese | MEDLINE | ID: mdl-32791797

ABSTRACT

Objective: To understand the clinical phenotype and spectrum of ATP7B gene mutation in children with Wilson's disease (WD). Methods: A total of 55 cases diagnosed with WD at the Children's Hospital Affiliated to Nanjing Medical University from June 2012 to June 2018 were taken as the research subject. ATP7B gene point mutation was detected by direct sequencing after PCR amplification. Heterozygous mutation in children was discovered by sequencing. Furthermore, the long segment mutation of exon was analyzed by multiplex ligation-dependent probe amplification (MLPA). Results: All 55 WD children had varying degree of liver damage symptoms. Among them, 2 cases had combined neurological symptoms. The positive rates of K-F ring (21%), 24-hour urine copper (97.7%), and ceruloplasmin were all abnormal. The results of ATP7B gene had identified 8 homozygous, 41 compound heterozygous and 6 heterozygous in 55 cases. Direct sequencing method had detected ten cases of ATP7B heterozygotes. In addition, MLPA analysis showed that other allele in four cases had a deletion of the ATP7B gene exon. In all cases, 35 different ATP7B gene mutations were detected, including 23 missense mutations, 3 frameshift mutations, 4 nonsense mutations, 3 exon deletions and 2 splicing changes. The most common allele mutation was c.2333G > T/p.R778L in exon 8, with an allele frequency of 36.54%, followed by c.2975C > T/p.P992L in exon 13, with an allele frequency of 14.42%. Conclusion: ATP7B gene c.2333G > T/p.R778L and c.2975C > T/p.P992L mutations are the most common mutations in children with WD in China. WD patients report shows that there are three long deletion mutations in the exon of the ATP7B gene. For WD children whose DNA sequencing is heterozygous ATP7B gene, it is suggested to further use MLPA method to detect deletion mutations of exons.


Subject(s)
Copper-Transporting ATPases/genetics , Hepatolenticular Degeneration , Child , China , DNA Mutational Analysis , Genotype , Hepatolenticular Degeneration/genetics , Humans , Mutation , Phenotype , Sequence Analysis, DNA
3.
Zhonghua Yi Xue Za Zhi ; 100(25): 1933-1936, 2020 Jul 07.
Article in Chinese | MEDLINE | ID: mdl-32629591

ABSTRACT

Objective: To explore the clinical features of post-viral-encephalitis autoimmune encephalitis (PVEAE). Methods: Ten cases of PVEAE, who were hospitalized in the Neurology Department of Peking Union Medical College Hospital (PUMCH) between November 2014 and October 2019, were retrospectively reviewed. Clinical manifestation, immunology, neuroradiology, treatment and outcomes were analyzed. Results: There were 5 males and 5 females, with a median age of 44 (18, 66) years. Of 9 cases, the median interval between the two onsets of encephalitis was 37 (24, 60) days, and there was no obvious interval in case 7. In viral encephalitis phase, the peak modified Rankin scale (mRS) was 4.5 (4.0, 5.0) and the remission mRS was 2.0 (1.0, 3.0). In autoimmune encephalitis (AE) phase, the peak mRS was 4.0 (3.0, 5.0). Symptoms of AE included mental and behavioral abnormalities (10/10), amnesia (10/10), motor disorders (5/10), autonomic dysfunction (5/10), speech disorders (4/10), seizures (2/10) and consciousness disturbance (2/10). On average, each case presented with 4 (2, 6) symptoms. In AE phase, the positive rate of anti-N-methyl-D-aspartate (anti-NMDA) receptor antibody in cerebrospinal fluid (CSF) was 80% (8/10), while in serum it was only 20% (2/10). Neuroimaging showed that in AE phase, the lesions expanded in 8 cases, remained unchanged in 1 case and shrank in 1 case. In AE phase, 10 cases received first line treatments, and 2 cases accepted long-course immunotherapy. After treatment, symptoms of 9 cases were obviously relieved. The mRS for short-term and long-term outcomes was 2.0 (1.0, 4.0) and 1.0 (0, 2.0), respectively. Conclusions: PVEAE might present with either typical biphasic course or monophasic/pseudo-monophasic course. In AE phase, anti-NMDA receptor antibody turned positive in most cases. Much importance should be attached to the recognition and diagnosis of PVEAE and treat it actively thereafter.


Subject(s)
Encephalitis, Viral , Encephalitis , Hashimoto Disease , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Receptors, N-Methyl-D-Aspartate , Retrospective Studies , Young Adult
4.
Phys Chem Chem Phys ; 22(13): 7058-7064, 2020 Apr 06.
Article in English | MEDLINE | ID: mdl-32196030

ABSTRACT

Polycrystalline Co2Ti1-xCrxO4 (0 ≤ x ≤ 0.2) inverse spinel ceramics have been synthesized via a sol-gel technique. The dc magnetization measurement in the field-cooled mode shows that negative magnetization could be observed until x reaches 0.2. The exchange constants are calculated using the ferrimagnetic Curie-Weiss fitting and the mean-field theory. This reveals that the strength of the inter sublattice magnetic interaction presents a non-monotonic trend with the increase in Cr content and reaches the minimum at x = 0.1, giving rise to the highest compensation temperature in the x = 0.1 sample. The applicability of the x = 0.1 sample is investigated in light of two prominent magnetic effects: (i) the stable magnetic switching effect indicates the potential applications in magnetic switching and data storage and (ii) the coexistence of normal and inverse magnetocaloric effects suggests a potential application in a constant temperature bath at 54 K.

5.
Zhonghua Yi Xue Za Zhi ; 97(27): 2120-2123, 2017 Jul 18.
Article in Chinese | MEDLINE | ID: mdl-28763887

ABSTRACT

Objective: To investigate the pathogenesis and management of the hydrocephalus in patients with systemic lupus erythematosus (SLE). Methods: Eight cases of hospitalized lupus patients with hydrocephalus in Peking Union Medical College Hospital from Jan 1990 to Mar 2017 were analyzed retrospectively.We collected the patients' medical records including medical history, CT and/or MRI images and analysis of cerebrospinal fluid via lumbar puncture in order to investigate the pathogenesis of the hydrocephalus in patients with SLE and summarize treatment experience. Results: All the 8 patients with SLE complicated with hydrocephalus were treated with steroids or immunosuppressive agents, and 5 cases were diagnosed with the central nervous system infection. Three cases received ventriculoperitoneal shunt, 5 cases received treatment of medicine.They all were followed up for 2-12 months, of which 3 cases were markedly effective, 1 case effective and 4 cases dead. Conclusion: The pathogenesis of lupus combined with hydrocephalus may have a certain relationship with the central infection, should take effective anti-infection treatment.We recommend cerebrospinal fluid shunt surgery intervention.


Subject(s)
Hydrocephalus/complications , Lupus Erythematosus, Systemic/complications , Cerebrospinal Fluid Shunts , Humans , Retrospective Studies , Ventriculoperitoneal Shunt
6.
Mol Biol Rep ; 40(4): 3381-8, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23271126

ABSTRACT

Hemangioma is a tumor that causes vascular endothelial cell hyperplasia, which commonly occur in newborns. Angiogenesis inhibitor targets the processes of angiogenesis, including the proliferation of vascular endothelial cells. A DNA sequence named Scl was designed, recombined into Pichia Pastoris, expressed by fermenting the engineered strain in a bioreactor, and purified the recombinant Scl by SP-sepharose fast flow. Scl can inhibit CAM angiogenesis. Only 1 µg of Scl significantly suppressed the growth of CAM blood vessel, similar to that of 25 µg of angiostatin. Scl showed a strong cytotoxicity on hemangioma cell (ATCC CRL No. 2587). After the drug acted for 24 h, the OD 570 measured value of the PBS control group averaged 1.873, whereas that of the Sc1 drug group was 0.692 (P < 0.01). Using the DeadEndTM Fluorometric TUNEL System, the detection results showed that 92 % of hemangioma cell apoptosis was observed in the Scl protein group, but only 1.3 % in the PBS control group (P < 0.01). After 2 weeks of treatment with the hemangioma model (cock's wattle) of the PBS group, 151 blood vessels with 100 views (40×) were obtained, whereas 250 in the PBS group (P < 0.01). During the two-week medication, the hemangioma model of the PBS group increased by 1.18 cm, whereas only 0.58 cm in the Scl drug group (P < 0.01).


Subject(s)
Endothelial Cells/drug effects , Hemangioma/drug therapy , Hyperplasia/genetics , Neovascularization, Pathologic/drug therapy , Peptides/administration & dosage , Angiogenesis Inhibitors/administration & dosage , Animals , Apoptosis/drug effects , Base Sequence , Cell Line, Tumor , Chickens/genetics , Chickens/growth & development , Endothelial Cells/pathology , Hemangioma/genetics , Hemangioma/pathology , Humans , Hyperplasia/drug therapy , Hyperplasia/pathology , Molecular Sequence Data , Neovascularization, Pathologic/genetics , Peptides/chemical synthesis , Peptides/genetics , Pichia
7.
Mol Biol Rep ; 39(5): 5805-10, 2012 May.
Article in English | MEDLINE | ID: mdl-22201022

ABSTRACT

α-amy gene amplified from barley genome was cloned into MCS of pGAP9K to generate pGAP9K-α-amy which was then transformed into Pichia pastoris GS115 by electroporation. Transformants with multi-copies and high expression for the foreign gene were selected on G418 containing plate and expression analysis. The fermentation was carried out in a 50 l bioreactor with 20 l working volume, using a high-density cell culture method by continuously feeding with 50% glycerol-0.8% PTM4 to the growing culture for 54 h at 30°C. Under the control of GAP promoter (pGAP), α-amy gene was constitutively expressed. At the end of the fermentation, the α-AMY expression reached 125 mg/l, while the biomass growth was 186 as measured by absorption of 600 nm. The secreted α-AMY was purified to 97.5% by SP-Sepharose FF ion-exchange chromatography and affinity purification. The recombinant α-AMY showed activity on hydrolysis of starch.


Subject(s)
Cell Culture Techniques/methods , Hordeum/enzymology , Pichia/growth & development , Pichia/metabolism , alpha-Amylases/metabolism , Biomass , Electrophoresis, Polyacrylamide Gel , Fermentation/drug effects , Gene Dosage/genetics , Hordeum/cytology , Hordeum/drug effects , Hydrolysis/drug effects , Peptones/pharmacology , Pichia/cytology , Pichia/drug effects , Polymerase Chain Reaction , Recombinant Proteins/isolation & purification , Starch/metabolism , Transformation, Genetic/drug effects , alpha-Amylases/isolation & purification
8.
Food Chem Toxicol ; 49(8): 1857-62, 2011 Aug.
Article in English | MEDLINE | ID: mdl-21586309

ABSTRACT

Compound K, i.e., 20-O-ß-d-glucopyranosyl-20(S)-protopanaxadiol, is the main metabolite of the protopanaxadiol type of ginseng saponin produced by intestinal bacteria after oral administration of ginseng extract. In the present study, the toxicity of compound K was evaluated in male and female dogs after 90 days continuous intravenous infusion. Beagle dogs were treated with compound K at doses of 6.7, 20 and 60 mg/kg/day, and observed for 90 days followed by recovery periods. Measurements included clinical observations, body weight, food consumption, temperature, electrocardiogram (ECG), hematology, blood chemistry, urinalysis, gross necropsy, organ weight and histopathology. Under the conditions, the clinical condition of the animals, body weights, body weight gains and food consumption were unaffected by compound K administration relative to the control group. Hematology, ECG data and urinalysis parameters were also unaffected. However, the hepatotoxicity was evident from the observation of multiple parameters, including histopathological evaluation of liver tissue upon necropsy as well as large increases in plasma levels of liver enzymes (alanine aminotransferase, ALT, Gamma-glutamyltranspeptidase, γ-GT, alkaline phosphatase,ALP) in groups receiving compound K (20 or 60 mg/kg/day), and this hepatoxicity might be reversible. In addition, the NOAEL of compound K is 6.7 mg/kg/day in this 90 days toxicity study.


Subject(s)
Ginsenosides/toxicity , Plant Extracts/toxicity , Toxicity Tests, Chronic/methods , Alanine Transaminase/blood , Alkaline Phosphatase/blood , Animals , Body Weight/drug effects , Dogs , Dose-Response Relationship, Drug , Eating/drug effects , Electrocardiography/drug effects , Female , Ginsenosides/chemistry , Injections, Intravenous , Liver/drug effects , Male , No-Observed-Adverse-Effect Level , Organ Size/drug effects , Panax/chemistry , Urinalysis
9.
Inflamm Res ; 56(3): 93-7, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17406805

ABSTRACT

OBJECTIVE: To investigate the expression of the Toll-like receptor (TLR) 4 in the brain after experimental subarachnoid haemorrhage (SAH) in rabbits. METHODS: A total of 52 rabbits were randomly divided into four groups: control group; day 3, day 5, and day 7 groups. Day 3, day 5, and day 7 groups were all SAH groups in which the rabbits were killed on day 3, 5, and 7, respectively. In SAH groups, autologous arterial blood was injected into cisterna magna twice on day 0 and day 2. Immunostaining and immunoblotting experiments were performed to detect the expression of TLR4 protein. Reverse-transcriptase polymerase chain reaction was used to analyze the presence and quantity of TLR4 mRNA. RESULTS: The expressions of TLR4 protein and mRNA were increased remarkably in SAH groups compared with the control group. The immunohistochemical staining demonstrated high level expression of TLR4 was present mainly in the endothelial cells of capillaries in the brain. CONCLUSION: Our results indicate that TLR4 expression is upregulated in the brain after experimental SAH.


Subject(s)
Brain/metabolism , Disease Models, Animal , Gene Expression Regulation , Subarachnoid Hemorrhage/metabolism , Toll-Like Receptor 4/metabolism , Animals , Immunohistochemistry , RNA, Messenger/genetics , Rabbits , Subarachnoid Hemorrhage/genetics , Subarachnoid Hemorrhage/pathology , Toll-Like Receptor 4/genetics
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