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1.
Sci Rep ; 14(1): 12194, 2024 05 28.
Article in English | MEDLINE | ID: mdl-38806542

ABSTRACT

Blood exchange therapy, specifically Whole blood exchange (WBE), is increasingly being utilized in clinical settings to effectively treat a range of diseases. Consequently, there is an urgent requirement to establish convenient and clinically applicable animal models that can facilitate the exploration of blood exchange therapy mechanisms. Our study conducted continuous WBE in rats through femoral and tail vein catheterization using dual-directional syringe pumps. To demonstrate the applicability of continuous WBE, drug-induced hemolytic anemia (DIHA) was induced through phenylhydrazine hydrochloride (PHZ) injection. Notability, the rats of DIHA + WBE group all survived and recovered within the subsequent period. After the implementation of continuous WBE therapy day (Day 1), the DIHA + WBE group exhibited a statistically significant increase in red blood cells (RBC) (P = 0.0343) and hemoglobin (HGB) levels (P = 0.0090) compared to DIHA group. The rats in the DIHA + WBE group exhibited a faster recovery rate compared to the DIHA group, indicating the successful establishment of a continuous blood exchange protocol. This experimental approach demonstrates not just promising efficacy in the treatment of DIHA and offers a valuable tool for investigating the underlying mechanisms of blood exchange. Furthermore, it has a great potential to the advancement of biomedical research such as drug delivery exploration.


Subject(s)
Phenylhydrazines , Animals , Rats , Male , Anemia, Hemolytic/blood , Anemia, Hemolytic/therapy , Disease Models, Animal , Hemoglobins , Erythrocytes/metabolism , Rats, Sprague-Dawley
2.
Quant Imaging Med Surg ; 14(1): 579-591, 2024 Jan 03.
Article in English | MEDLINE | ID: mdl-38223078

ABSTRACT

Background: Pneumonia can be anatomically classified into lobar, lobular, and interstitial types, with each type associated with different pathogens. Utilizing artificial intelligence (AI) to determine the anatomical classifications of pneumonia and assist in refining the differential diagnosis may offer a more viable and clinically relevant solution. This study aimed to develop a multi-classification model capable of identifying the occurrence of pneumonia in patients by utilizing case-specific computed tomography (CT) information, categorizing the pneumonia type (lobar, lobular, and interstitial pneumonia), and performing segmentation of the associated lesions. Methods: A total of 61 lobar pneumonia patients, 60 lobular pneumonia patients, and 60 interstitial pneumonia patients were consecutively enrolled at our local hospital from June 2020 and May 2022. All selected cases were divided into a training cohort (n=135) and an independent testing cohort (n=46). To generate the ground truth labels for the training process, manual segmentation and labeling were performed by three junior radiologists. Subsequently, the segmentations were manually reviewed and edited by a senior radiologist. AI models were developed to automatically segment the infected lung regions and classify the pneumonia. The accuracy of pneumonia lesion segmentation was analyzed and evaluated using the Dice coefficient. Receiver operating characteristic curves were plotted, and the area under the curve (AUC), accuracy, precision, sensitivity, and specificity were calculated to assess the efficacy of pneumonia classification. Results: Our AI model achieved a Dice coefficient of 0.743 [95% confidence interval (CI): 0.657-0.826] for lesion segmentation in the training set and 0.723 (95% CI: 0.602-0.845) in the test set. In the test set, our model achieved an accuracy of 0.927 (95% CI: 0.876-0.978), precision of 0.889 (95% CI: 0.827-0.951), sensitivity of 0.889 (95% CI: 0.827-0.951), specificity of 0.946 (95% CI: 0.902-0.990), and AUC of 0.989 (95% CI: 0.969-1.000) for pneumonia classification. We trained the model using labels annotated by senior physicians and compared it to a model trained using labels annotated by junior physicians. The Dice coefficient of the model's segmentation improved by 0.014, increasing from 0.709 (95% CI: 0.589-0.830) to 0.723 (95% CI: 0.602-0.845), and the AUC improved by 0.042, rising from 0.947 to 0.989. Conclusions: Our study presents a robust multi-task learning model with substantial promise in enhancing the segmentation and classification of pneumonia in medical imaging.

3.
Ecotoxicol Environ Saf ; 243: 114001, 2022 Sep 15.
Article in English | MEDLINE | ID: mdl-36027710

ABSTRACT

Heavy metal pollution is a major threat to agricultural produce and it can pose potential ecological risks which subsequently impacts on human health. Strawberries are an economically important produce of China. The intrinsic link of heavy metal pollution risk in the soil-strawberry ecosystem is of concern. In this study, the pollution index of heavy metal pollutants in farmlands of different provinces were evaluated, and the results showed significantly high levels of cadmium. In addition, Nemerow integrated pollution index analysis showed that low-pollution farmlands only accounted for 14.07% of the total arable land area. Then, the transfer factors were used to calculate the migration of heavy metals from the soil into strawberries. The results showed that cadmium and nickel were relatively high in strawberries from the Guangxi province. Similar results were found for mercury in Jiangxi Province. The pollution index of single food pollution also showed that mercury in strawberries from Jiangxi Province was at a moderate pollution level. The comprehensive pollution index indicated that heavy metal pollution in strawberries in Central China may be severe. In addition, spatial clustering analysis showed that cadmium, chromium, lead, arsenic and zinc in strawberries had significant hotspot clustering in central, south and southwest China. Finally, our studies also suggested that the risk of carcinogenic and non-carcinogenic diseases was higher in the (2, 4] years age group than in other age groups. People in Yunnan Province were also found to have a higher non-carcinogenic risk than those in other provinces and cities in China. This study provides a comprehensive view of the potential risks of heavy metal contamination in strawberries, which could provide assistance in the design of regulatory and risk management programs for chemical pollutants in strawberries, thus ensuring the safety of consumption of these edible fruits.


Subject(s)
Environmental Pollutants , Fragaria , Mercury , Metals, Heavy , Soil Pollutants , Cadmium/analysis , China , Ecosystem , Environmental Monitoring , Environmental Pollutants/analysis , Humans , Mercury/analysis , Metals, Heavy/analysis , Risk Assessment , Soil , Soil Pollutants/analysis
4.
Am J Physiol Gastrointest Liver Physiol ; 323(1): G9-G20, 2022 06 01.
Article in English | MEDLINE | ID: mdl-35411804

ABSTRACT

Anemia is a common complication of hepatitis B-related acute-on-chronic liver failure (HB-ACLF). Eryptosis, a suicidal erythrocyte death characterized by phosphatidylserine (PS) externalization and red blood cell-derived microparticle (RMP) generation, decreases erythrocyte lifespan. Herein, we investigated whether enhanced eryptosis is involved in the anemia pathophysiology associated with HB-ACLF. PS exposure, cell volume, cytosolic Ca2+, and reactive oxygen species (ROS) production were determined using flow cytometry. RMPs were extracted using a polyethylene glycol (PEG)-based method. We found that hemoglobin (Hb) and hematocrit (Hct) were significantly lower in patients with HB-ACLF than in healthy controls (HC), patients with chronic hepatitis B (CHB), and patients with cirrhosis. The direct antiglobulin test positive rate was 75.9% in patients with HB-ACLF while its intensity was associated with anemia. The ratio of abnormal erythrocytes was higher in patients with HB-ACLF than in HC, CHB, and cirrhosis. The percentage of PS-exposed erythrocytes was higher in patients with HB-ACLF (2.07 ± 0.11%) compared with HC (0.37 ± 0.05%), CHB (0.38 ± 0.03%), and cirrhosis (0.38 ± 0.04%). The cytosolic Ca2+ and ROS abundance were also higher in patients with HB-ACLF compared with HC, patients with CHB, and patients with cirrhosis, and were inversely correlated with the anemia in patients with HB-ACLF. PS exposure of erythrocytes collected from HC was significantly pronounced following incubation in plasma from patients with HB-ACLF compared with incubation in plasma from HC. The protein concentration and RMPs size significantly increased in patients with HB-ACLF compared with HC. Thus, the anemia in patients with HB-ACLF is associated with increased eryptosis, which is partially triggered by increased cytosolic Ca2+ and oxidative stress.NEW & NOTEWORTHY Acute chronic liver failure (ACLF) is a critical syndrome characterized by multiple organ failures and high short-term mortality. A common complication of HB-ACLF is anemia, however, the mechanism of anemia in HB-ACLF remains to be elucidated. We confirm that the accelerated eryptosis is involved in the pathophysiology of anemia associated with HB-ACLF, which progressively aggravates the clinical outcome. Our study illustrates the mechanism regarding the anemia pathogenesis of HB-ACLF, which may be utilized further toward therapeutic ends.


Subject(s)
Acute-On-Chronic Liver Failure , Anemia , Eryptosis , Hepatitis B, Chronic , Hepatitis B , Acute-On-Chronic Liver Failure/complications , Anemia/complications , Hepatitis B/complications , Hepatitis B, Chronic/complications , Humans , Liver Cirrhosis/complications , Phosphatidylserines/metabolism , Reactive Oxygen Species/metabolism
5.
Talanta ; 241: 123240, 2022 May 01.
Article in English | MEDLINE | ID: mdl-35065346

ABSTRACT

Molecularly imprinted polymers (MIPs) are artificial chemical receptors, and can recognize template molecules with a high selectivity and affinity. As "antibody mimics", MIPs have been widely studied in various fields. However, the general applicability of MIPs is limited by the type of functional monomers. Herein, we developed caffeic acid (CA, a natural polyphenol) as novel a functional monomer. An innovative poly(caffeic acid)-coated molecularly imprinted magnetic nanoparticles (PCA-MIMN) with transferrin (TRF) as a model glycoprotein template was fabricated by autoxidation of CA with hexamethylenediamine (HMDA) in an aerobic environment as imprinted layer. The successful fabrication of PCA-MIMN was proved in detail by diversified characterization. The PCA-MIMN exhibited not only outstanding binding affinity and specificity for target glycoprotein, but also excellent hydrophilicity due to the externally generous hydrophilic groups. To evaluate the preeminent performance, the PCA-MIMN was linked with pH-triggered allochroic-graphene oxide (AGO), which was used for determination of TRF in real samples. The proposed PCA-MIMN linked AGO strategy exhibited ultrahigh sensitivity with limit of detection of 0.38 pg mL-1 for TRF. Finally, the proposed strategy was successfully applied in determination of TRF in spiked human serum sample with recovery and relative standard deviation in the range of 97.2%-103.9% and 4.6%-5.8%, respectively. This work demonstrates that the "autoxidation of CA with HMDA" may be a universal tool for synthesis of highly specific MIPs, and the type of functional monomers will increase exponentially due to the presence of numerous polyphenols in nature.


Subject(s)
Magnetite Nanoparticles , Molecular Imprinting , Adsorption , Caffeic Acids , Glycoproteins , Humans , Magnetite Nanoparticles/chemistry , Polymers/chemistry
7.
Transfus Apher Sci ; 60(5): 103201, 2021 Oct.
Article in English | MEDLINE | ID: mdl-34238707

ABSTRACT

BACKGROUND: Multiple factors contribute to anemia in patients with Hepatitis B virus (HBV)related acute-on-chronic liver failure (ACLF); however, the mechanism is unclear. The purpose of this study was to evaluate the clinical significance of the direct antiglobulin test (DAT) in patients with HBV related ACLF. METHODS: DAT was used to detect immunoglobulins and/or complement proteins on the surface of erythrocytes. RESULTS: We recruited 78 HBV-associated ACLF patients, 30 chronic hepatitis B(CHB)patients and 40 healthy people between October 2015 and May 2016. In HBV related ACLF patients, the hemoglobin concentration, number of erythrocytes, and hematocrit value were significantly lower, while the erythrocyte distribution width was significantly higher, compared to patients with CHB and healthy controls (HCs) (P < 0.001). The rates of DAT positivity in HBV related ACLF patients, CHB patients, and HCs were 62.8 %, 13.3 %, and 0%, respectively. DAT-positive ACLF patients exhibited lower Hb levels, older average age, as well as higher total bilirubin, alanine aminotransferase, and complement component 3 levels compared to DAT-negative patients. CONCLUSIONS: HBV related ACLF patients showed significant alterations in erythrocyte parameters, possibly reflecting disease development and severity. The high presence of erythrocyte autoantibodies suggested that immunologic clearance of erythrocytes contributed to multifactorial anemia in HBV related ACLF patients.


Subject(s)
Acute-On-Chronic Liver Failure/blood , Coombs Test/methods , Hemoglobins/analysis , Hepatitis B, Chronic/blood , Acute-On-Chronic Liver Failure/complications , Adult , Aged , Alanine Transaminase/blood , Bilirubin/blood , Complement System Proteins , Erythrocytes/cytology , Female , Hepatitis B, Chronic/complications , Humans , Male , Middle Aged , Reproducibility of Results
8.
Dalton Trans ; 50(23): 8120-8126, 2021 Jun 15.
Article in English | MEDLINE | ID: mdl-34021298

ABSTRACT

Introducing electronically active organic components into lower dimensional metal halide compounds is an effective strategy to improve the electronic properties of hybrid metal halide materials. We have previously used this strategy to explore hybrid halides with tetrathiafulvalenes (TTFs) and a series of lead iodides and bismuth halides were isolated. The electronic properties were improved notably using this modification. In this work, we expand the study of TTF based main-group metal halides to double metal halides with mixed lead and copper transition metals. Two hybrid TTF-lead-cuprous iodides, formulated as [TTF]5[Pb2Cu2I10]·H2O (1) and [TTF]2[PbCu2I6] (2), and two monometal analogues of [TTF]2[Cu4I6]·H2O (3) and [TTF]2[Ag4I6] (4) were crystallographically characterized. The anion of 1 is a 0D cluster, while that of the others is a 1D chain structure. The anion structures of 1-4 are novel and are reported for the first time. The TTF moieties are stacked to form a 2D framework in 1 and 1D columns in 2-4. We found that the semiconductor properties of the hybrids are related to electron donation from an anion to a cation. The electronic state of the TTF cations is another significant factor that affects the electronic properties of the materials. More notably, this work proved that the conductivity and photoconductivity of the mixed metal iodides are superior to those of the monometal iodides.

9.
Sci Rep ; 11(1): 2505, 2021 01 28.
Article in English | MEDLINE | ID: mdl-33510312

ABSTRACT

Red blood cells (RBCs) stressed by high temperature are similar to senescent or damaged RBCs in pathological conditions. RBCs can be efficiently labelled with 18F-fluorodeoxyglucose (FDG). The aim of this study was to assess stressed RBCs erythrophagocytosis and organ distribution in vivo with the application of 18F-FDG PET/CT. RBCs were induced under high temperature (48 °C) to prepare stressed RBCs. Fluorescence-activated cell sorting (FACS) was used to analyse reactive oxygen species (ROS) generation, intracellular Ca2+ concentration and membrane phosphatidylserine (PS) externalization of RBCs. 18F-FDG was used to label RBCs and assess the erythrophagocytosis. Finally, 18F-FDG PET/CT was applied to reveal and measure the organ distribution of stressed RBCs in mice. Compared with untreated RBCs, stressed RBCs decreased in cell volume and increased in ROS level, intracellular Ca2+ concentration, and PS exposure. RBCs could be labelled by 18F-FDG. Stressed RBCs tended to be phagocytosed by macrophages via assessment of FACS and radioactivity. 18F-FDG PET/CT imaging showed that stressed RBCs were mainly trapped in spleen, while untreated RBCs remained in circulation system. Thus, stressed RBCs can be effectively labelled by 18F-FDG and tend to be trapped in spleen of mice as assessed by PET/CT.


Subject(s)
Erythrocytes/metabolism , Fluorodeoxyglucose F18 , Positron Emission Tomography Computed Tomography , Stress, Physiological , Animals , Calcium/metabolism , Mice , Organ Specificity , Phagocytosis , Reactive Oxygen Species/metabolism , Spleen/blood supply , Spleen/metabolism , Temperature , Tissue Distribution
10.
Transfus Apher Sci ; 60(1): 102930, 2021 Feb.
Article in English | MEDLINE | ID: mdl-32933846

ABSTRACT

Liver-targeted cargo delivery possesses great potential for the treatment of liver disease. It is urgent to find an efficient and biocompatible liver targeted delivery system. This study focused on the liver targeting properties of erythrocyte ghosts and its possible mechanism. Herein, we optimized conditions to fabricate human and mouse erythrocyte ghosts with sufficient room capable of incorporating various model substances. Erythrocyte ghosts are biocompatible cargo carriers because it is derived from autologous red blood cells (RBCs), and the cell size, zeta potential, and biconcave-disk shape of the ghosts were consistent with those of RBCs. An in vivo imaging system and positron emission tomography/computed tomography imaging showed that the ghosts were captured mainly in the liver by intravenous injection of fluorescence or 18F-fluorodeoxyglucose (FDG)-labelled ghosts into mice. In contrast, the main concentration of naked octreotide was trapped in the lungs while naked 18F-FDG was trapped in the heart. However, the concentration of cargo-loaded ghosts decreased significantly in the liver in macrophage-depleted mice. Accordingly, in vitro experiments showed that higher phosphatidylserine exposure was observed in the ghosts (38.9 %) compared to normal erythrocytes (0.69 %), and the phagocytic activity of the macrophage RAW 264.7. on the ghosts was significantly higher than that of normal erythrocytes (p < 0.001). Together they indicate that erythrocyte ghosts show liver targeting properties, and possibly owing to macrophage phagocytosis. This promising and effective therapeutic delivery system may provide therapeutic benefits for liver disease.


Subject(s)
Erythrocyte Count/methods , Macrophages/metabolism , Humans
11.
ACS Appl Mater Interfaces ; 12(47): 52615-52623, 2020 Nov 25.
Article in English | MEDLINE | ID: mdl-33170613

ABSTRACT

Metal-organic frameworks (MOFs) have aroused great interest as lithium-ion battery (LIB) electrode materials. In this work, we first report that a pristine three-dimensional tetrathiafulvalene derivatives (TTFs)-based zinc MOF, formulated [Zn2(py-TTF-py)2(BDC)2]·2DMF·H2O (1) (py-TTF-py = 2,6-bis(4'-pyridyl)tetrathiafulvalene and H2BDC = terephthalic acid), can work as a high-performance electrode material for rechargeable LIBs. The TTFs-Zn-MOF 1 electrode displayed a high discharge specific capacity of 1117.4 mA h g-1 at a current density of 200 mA g-1 after 150 cycles along with good reversibility. After undergoing elevated discharging/charging rates, the electrode showed superior lithium storage performance in the extreme case of 20 A g-1 and could finally recover the capability when the current rate was back to 200 mA g-1. Particularly, specific capacities of 884.2, 513.8, and 327.8 mA h g-1 were reached at high current densities of 5, 10, and 20 A g-1 after 180, 175, and 300 cycles along with good reversibility, respectively. Such an excellent performance is first reported for the LIB anode materials. TTFs-Zn-MOF 2, namely, [Zn2(py-TTF-py) (BDC)2]·DMF·2H2O (2), was prepared as a contrast to explore the relationship between the structures of the electrode materials and the electrochemical properties. Based on the structural analysis of 1 and 2 and ex situ X-ray photoelectron spectroscopy, the TTF moiety and the twofold TTF pillar play a key role in the excellent electrochemical performance. The full cell of MOF 1 with NMC 622 delivered the capacity of 131.9 mA h g-1 at 100 mA g-1 with the Coulombic efficiency of 99.45% after 70 cycles and exhibited the tolerance to high-current operation.

12.
Inorg Chem ; 59(7): 5161-5169, 2020 Apr 06.
Article in English | MEDLINE | ID: mdl-32186867

ABSTRACT

Most large organic cations in the low-dimensional hybrid halide perovskites deteriorate the photoelectric conversion efficiency of the cells. Integrating electronically active organic components into hybrid metal halides is an effective method to improve their photoelectric properties. In this work, a series of compounds obtained by hybridizing redox-active tetrakis(methylthio)tetrathiafulvalene (TMT-TTF) with bismuth chloride, formulated as [TMT-TTF]4[Bi6Cl22] (1 and 1'), [TMT-TTF]3[Bi4Cl16] (2), [TMT-TTF]2[Bi3Cl13] (3), [TMT-TTF]2[Bi2Cl10] (4), and {[TMT-TTF][Bi2Cl8]}n (5), were crystallographically characterized. These hybrids exhibit changeable oxidation states of the TTF moiety. The radical cation TTF•+ exists in 1 and 1', while a mixed-valence TTF•+/TTF2+ appears in 2 that has never been documented in any compounds and the dication TTF2+ exists in 3-5 that has never been introduced into hybrid organic-inorganic materials. The different charged states of the TTF cations lead to various degrees of connectivity of metal chloride anions, which exert a significant effect on the cation-anion arrangement and result in different supramolecular interactions between TMT-TTF and between cations and anions. The changeable oxidation states of the TTF moiety and varying degrees of metal chloride connectivity provide a good comparison among these hybridized bismuth chlorides. The order of conductivity is 2 > 1 > 1' > 3 ≈ 4 ≫ 5, which results from the synergistic effect of different oxidation states, the packing of TMT-TTF cations, and back charge transfer from the Bi-Cl anion to the TMT-TTF cation. Notably, the electrical conductivity and carrier mobility can be modulated with the fact that compound 2 has the highest performances in the dark, while in light, these properties of 1 and 1' are in turn higher than that of 2. The order of the photocurrent densities is in accordance with the increase of carrier mobility under irradiation of light. This work is the first systematic study of hybrid metal halides with various oxidation states of TTFs and presents a clear structure-property relationship and offers a fresh view on the design of new perovskite materials at the molecular level.

13.
Inorg Chem ; 58(23): 15824-15831, 2019 Dec 02.
Article in English | MEDLINE | ID: mdl-31710209

ABSTRACT

Inorganic CuSCN and organic tetrathiafulvalene derivatives (TTFs) have been exploited as hole-transport materials (HTM) in hybrid perovskite solar cells. To develop new HTM, we herein report two hybrid materials incorporating redox-active TTFs with CuSCN framework (TTFs-CuSCN). Single-crystal analysis showed that compound [Cu2(py-TTF-py)(SCN)2] (1) is three-dimensional (3D) and compound [Cu(py-TTF-py)(SCN)] (2) is two-dimensional (2D) (py-TTF-py = 2,6-bis(4'-pyridyl)tetrathiafulvalene). There are covalent coordination interactions between CuSCN and py-TTF-py and short S···S contacts between the py-TTF-py ligands for both compounds. Besides, C···S contacts exist between py-TTF-py ligands of the neighboring 2D networks in 2, which facilitate the charge transfer and supply efficient multidimensional pathways for carrier migration. As a result, 2 presented better semiconductor performance in comparison with that of 1. The performance of 2 related to the HTMs could be significantly improved by modulating the electronic state of the TTFs-CuSCN framework via oxidative doping. The iodine-doped 2D material (2-I2) gives the most excellent conductivity and carrier mobility, which might be a potential new HTM.

14.
Acta Crystallogr Sect E Struct Rep Online ; 69(Pt 2): o211, 2013 Feb 01.
Article in English | MEDLINE | ID: mdl-23424493

ABSTRACT

In the crystal structure of the title salt, C(9)H(9)N(2)O(2) (+)·Cl(-), the cations and anions are linked into chains parallel to [021] by O-H⋯Cl and N-H⋯Cl hydrogen bonds.

15.
Dalton Trans ; 41(8): 2340-5, 2012 Feb 28.
Article in English | MEDLINE | ID: mdl-22186681

ABSTRACT

Three new metal(II) 2-pyrazinephosphonates have been synthesized by hydrothermal reactions based on 2-pyrazinephosphonic acid (C(4)H(3)N(2)PO(3)H(2)1) as ligand, namely, Zn(C(4)H(3)N(2)PO(3)) (2), Cd[(C(4)H(3)N(2)PO(3))(H(2)O)] (3) and Cd[(C(4)H(3)N(2)PO(3)H)Cl]·H(2)O (4). In compound 2, the O-P-O bridged inorganic layers are pillared by pyrazinyl groups into a three-dimensional network. In compound 3, the {CdO(5)N} and {CPO(3)} polyhedra are interconnected via edge and corner-sharing into a metal phosphonate layer. In compound 4, the {Cd(2)Cl(2)} dimers are linked by O-P-O bridges into a one-dimensional double chain, and the chains are joined into a layer by pyrazinyl groups. Here we employ pyrazinephosphonic acids as structure directing motifs to form extended structures and materials with interesting luminescent properties. The luminescent properties studied have also been described.

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