Functional CRISPR screens in T cells reveal new opportunities for cancer immunotherapies.

T cells are fundamental components in tumour immunity and cancer immunotherapies, which have made immense strides and revolutionized cancer treatment paradigm. However, recent studies delineate the predicament of T cell dysregulation in tumour microenvironment and the compromised efficacy of cancer immunotherapies. CRISPR screens enable unbiased interrogation of gene function in T cells and have revealed functional determinators, genetic regulatory networks, and intercellular interactions in T cell life cycle, thereby providing opportunities to revamp cancer immunotherapies. In this review, we briefly described the central roles of T cells in successful cancer immunotherapies, comprehensively summarised the studies of CRISPR screens in T cells, elaborated resultant master genes that control T cell activation, proliferation, fate determination, effector function, and exhaustion, and highlighted genes (BATF, PRDM1, and TOX) and signalling cascades (JAK-STAT and NF-κB pathways) that extensively engage in multiple branches of T cell responses. In conclusion, this review bridged the gap between discovering element genes to a specific process of T cell activities and apprehending these genes in the global T cell life cycle, deepened the understanding of T cell biology in tumour immunity, and outlined CRISPR screens resources that might facilitate the development and implementation of cancer immunotherapies in the clinic.

Extracellular vesicle-derived non-coding RNAs in remodeling melanoma.

Melanoma is one of the most lethal cutaneous malignancies. Despite great advances in radiotherapy, chemotherapy, and immunotherapy, the survival rate and prognosis of patients with melanoma remain poor. The abundant and sophisticated reciprocal communication network between melanoma cells and non-tumor cells contributes to the high heterogeneity of the melanoma microenvironment and is intimately related to varying treatment responses and clinical courses. Extracellular vesicles (EVs) are membrane structures generated by nearly all cell types. EVs contain biologically active molecules, mainly comprising proteins, lipids, and RNAs, and undoubtedly play multifaceted roles in numerous diseases, represented by melanoma. Non-coding RNAs (ncRNAs) mainly encompass long non-coding RNAs, microRNAs, and circular RNAs and constitute the majority of the human transcriptome. Multiple ncRNAs encapsulated in EVs coordinate various pathophysiological processes in melanoma. This review summarizes the mechanisms by which EV-ncRNAs modulate biological behaviors and immunity, and their potential diagnostic and therapeutic applications in melanoma. Undoubtedly, further insight into EV-ncRNAs and their functions in melanoma will contribute to the clinical treatment of melanoma and the implementation of precision medicine.

Neutrophil extracellular trap-related mechanisms in acne vulgaris inspire a novel treatment strategy with adipose-derived stem cells.

Acne vulgaris is a type of chronic skin disorder caused by Propionibacterium acnes (P. acnes). Neutrophil extrinsic traps (NETs) play key role in many types of inflammatory skin diseases. Adipose-derived stem cells (ADSCs) was reported modulate immune responses and neutrophil activity. Here, we explored the potential role of ADSCs and the potential mechanism associated with neutrophil extracellular traps (NETs) in relieving acne vulgaris. In the P. acnes-infected ear skin model, histological staining was used to evaluate the inflammatory infiltration and NET formation in control, P. acnes, and P. acnes + ADSCs groups. Besides, western blot was used to detect the expression levels of cit-H3, MPO, and Nrf2 in ear tissue. In vitro, the immunofluorescence staining of MPO and cit-H3, and SYTOX green staining were performed to measure the NET formation. CCK-8 assay, EdU staining, and wound healing assay were used to detect the proliferation and migration abilities of keratinocytes. ELISA assay was utilized to detect the secretion of inflammatory cytokines. In P. acnes-infected ear skin, ADSC treatment significantly attenuated inflammation and NET formation via activating Nrf2 signaling pathway. In vitro, the conditioned medium of ADSCs reduced the formation of P. acne-induced NETs. Besides, ADSCs could inhibit that the NETs efficiently promoted the proliferation, migration, and inflammatory cytokine secretion of keratinocytes. Our study suggested that ADSCs could attenuate P. acne-related inflammation by inhibiting NET formation. This study provides a novel therapeutic perspective of ADSCs in combating acne vulgaris.

Contribution of Insulin Resistance and ß Cell Dysfunction to Gestational Diabetes Stratified for Pre-pregnant Body Mass Index.

The objective of the study was to evaluate the contribution of insulin resistance and ß cell dysfunction to gestational diabetes mellitus (GDM) in Chinese women stratified by pre-pregnant body mass index (BMI). A total of 847 pregnant women were enrolled. They were divided into low BMI and high BMI groups according to the median of pre-pregnancy BMI. The homeostasis model assessment of insulin resistance (HOMA-IR) and ß cell function (HOMA-ß), Matsuda index, and 60-min insulinogenic index (IGI60) were used to evaluate insulin resistance and ß cell function. In all the participants, 150 (17.71%) were diagnosed with GDM. ROC analyses showed that in the low BMI group, the association of ß cell dysfunction (IGI60 or HOMA-ß) with GDM was stronger than that of insulin resistance (Matsuda index or HOMA-IR), while in the high BMI group, the association of ß cell dysfunction with GDM was weaker than that of insulin resistance (all P < 0.05). Among all GDM patients, 47.33% demonstrated predominant insulin resistance (Matsuda index < 25th percentile), and 46% had predominant ß cell defect (IGI60 < 25th percentile). In the low BMI group, 15.09% of GDM patients demonstrated predominant insulin resistance, and 62.26% of GDM patients had predominant ß cell defect, whereas in the high BMI group, 64.95% of GDM patients demonstrated mainly insulin resistance and 36.08% of GDM patients had mainly ß cell defect. In women with low BMI, ß cell dysfunction is the major etiologic factor, whereas, in women with high BMI, insulin resistance is the predominant etiologic factor in the development of GDM.

Predicting pediatric Crohn's disease based on six mRNA-constructed risk signature using comprehensive bioinformatic approaches.

Crohn's disease (CD) is a recurrent, chronic inflammatory condition of the gastrointestinal tract which is a clinical subtype of inflammatory bowel disease for which timely and non-invasive diagnosis in children remains a challenge. A novel predictive risk signature for pediatric CD diagnosis was constructed from bioinformatics analysis of six mRNAs, adenomatosis polyposis downregulated 1 (APCDD1), complement component 1r, mitogen-activated protein kinase kinase kinase kinase 5 (MAP3K5), lysophosphatidylcholine acyltransferase 1, sphingomyelin synthase 1 and transmembrane protein 184B, and validated using samples. Statistical evaluation was performed by support vector machine learning, weighted gene co-expression network analysis, differentially expressed genes and pathological assessment. Hematoxylin-eosin staining and immunohistochemistry results showed that APCDD1 was highly expressed in pediatric CD tissues. Evaluation by decision curve analysis and area under the curve indicated good predictive efficacy. Gene Ontology, Kyoto Encyclopedia of Genes and Genomes and gene set enrichment analysis confirmed the involvement of immune and cytokine signaling pathways. A predictive risk signature for pediatric CD is presented which represents a non-invasive supplementary tool for pediatric CD diagnosis.

A cold-responsive liquid crystal elastomer provides visual signals for monitoring a critical temperature decrease.

Critical temperature indicators have been extensively utilized in various fields, ranging from healthcare to food safety. However, the majority of the temperature indicators are designed for upper critical temperature monitoring, indicating when the temperature rises and exceeds a predefined limit, whereas stringently demanded low critical temperature indicators are scarcely developed. Herein, we develop a new material and system that monitor temperature decrease, e.g., from ambient temperature to the freezing point, or even to an ultra-low temperature of -20 °C. For this purpose, we create a dynamic membrane which can open and close during temperature cycles from high temperature to low temperature. This membrane consists of a gold-liquid crystal elastomer (Au-LCE) bilayer structure. Unlike the commonly used thermo-responsive LCEs which actuate upon temperature rise, our LCE is cold-responsive. This means that geometric deformations occur when the environmental temperature decreases. Specifically, upon temperature decrease the LCE creates stresses at the gold interface by uniaxial deformation due to expansion along the molecular director and shrinkage perpendicular to it. At a critical stress, optimized to occur at the desired temperature, the brittle Au top layer fractures, which allows contact between the LCE and material on top of the gold layer. Material transport via cracks enables the onset of the visible signal for instance caused by a pH indicator substance. We apply the dynamic Au-LCE membrane for cold-chain applications, indicating the loss of the effectiveness of perishable goods. We anticipate that our newly developed low critical temperature/time indicator will be shortly implemented in supply chains to minimize food and medical product waste.

Reversible Perspiring Artificial "Fingertips".

Fingertip perspiration is a vital process within human predation, to which the species owes its survival and its biological success. In this paper, the unique human ability of extensive perspiration and controlled friction in self-assembled cholesteric liquid crystals is recreated, mimicking the natural processes that occur in the dermis and epidermis of human skin. This is achieved by inducing porosity in responsive, liquid-bearing material through the controlled-polymerization phase-separation process. The unique topography of human fingerprints is further emulated in the materials by balancing the parallel chirality-induced force and the perpendicular substrate-anchoring force during synthesis. As a result, artificial fingertips are capable of secreting and re-absorbing liquid upon light illumination. By demonstrating the function of the soft material in a tribological aspect, it exhibits a controllable anti-sliding property comparable to human fingertips and subsequently attains a higher degree of biomimicry. This biomimetic fingertip is envisioned being applied in a multitude of fields, ranging from biomedical instruments to interactive, human-like soft robotic devices.

Perspiring Soft Robotics Skin Constituted by Dynamic Polarity-Switching Porous Liquid Crystal Membrane.

Secretion of functional fluids is essential for affecting surface properties in ecosystems. The existing polymer membranes that mimic human skin functions are limited to secreting, either apolar or polar, liquid. However, the development of membranes that grant exchange liquid with different polarities remains a grand challenge. This process is prohibited by the mismatch of the polarity between the carrier polymer and the loaded liquid. To conquer this limitation, an innovative strategy is reported to dynamically switch the polarity of the porous membrane, thereby empowering the exchange of apolar liquid with polar liquid and vice versa. This approach incorporates a benzoic acid derivative into the original apolar polymer network. The benzoic acid dimerizes and forms hydrogen bonds, which supports the molecular alignment, but can be broken into the ionic state when subjected to alkaline treatment, changing the polarity of themembrane. Consequently, the apolar liquid can be replaced with a more polar one. This polar liquid is ejected upon safe-dose UV illumination from the membrane. Reabsorption occurs on demand by illumination of visible light or when left in contact with the membrane, spontaneously in the dark. Based on this, the consumed membrane is replenished with the same or different exchanging liquid.

The Distribution Characteristics and Potential Risk Assessment of Lead in the Soil of Tieguanyin Tea Plantations in Anxi County, China.

Assessing the distribution and risks associated with the soil lead content in the Tieguanyin tea plantations of Anxi County is critical, given the county's significance as the primary Tieguanyin tea production area in Fujian Province. This study examined the distribution characteristics of soil lead in Anxi County's tea plantations according to the Kriging spatial interpolation of the parameters of the semivariance function of the exponential model. Moreover, the sources of lead content were analyzed, considering geological backgrounds and anthropogenic influences. Ecological risks and the issuance of early warnings were also assessed. The soil lead content in the rocks of the Tieguanyin tea plantations in Anxi County followed the order: andesite > dacite > rhyolite > granite. The soil lead content gradually decreased from the center toward the east and west, forming four distinct north-south parallel zones. High-lead-content areas were identified at the border of Jiandou, Bailai, and Hushang; in the central part of Lutian; and in the southern part of Huqiu. The high levels of soil lead in the tea plantations possibly originated from industrial and mining activities, automobile exhaust, and agricultural activities. The distribution of single-factor pollution indices and potential risk evaluation based on the Soil Environmental Quality Standard, Environmental Technical Conditions for Tea Production Area, and Environmental Technical Conditions for Organic Tea Production Area indicated that the soil in Tieguanyin tea plantations in Anxi County was clean and safe for tea cultivation.

NcRNA: key and potential in hearing loss.

Hearing loss has an extremely high prevalence worldwide and brings incredible economic and social burdens. Mechanisms such as epigenetics are profoundly involved in the initiation and progression of hearing loss and potentially yield definite strategies for hearing loss treatment. Non-coding genes occupy 97% of the human genome, and their transcripts, non-coding RNAs (ncRNAs), are widely participated in regulating various physiological and pathological situations. NcRNAs, mainly including micro-RNAs (miRNAs), long-stranded non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), are involved in the regulation of cell metabolism and cell death by modulating gene expression and protein-protein interactions, thus impacting the occurrence and prognosis of hearing loss. This review provides a detailed overview of ncRNAs, especially miRNAs and lncRNAs, in the pathogenesis of hearing loss. We also discuss the shortcomings and issues that need to be addressed in the study of hearing loss ncRNAs in the hope of providing viable therapeutic strategies for the precise treatment of hearing loss.

A combined aging and immune prognostic signature predict prognosis and responsiveness to immunotherapy in melanoma.

Background: Melanoma is the most lethal, and one of the most aggressive forms of cutaneous malignancies, which poor response to treatment has always puzzled clinicians. As is known to all, aging and immune microenvironment are two crucial factors impacting melanoma biological progress through the tumor microenvironment (TME). However, reliable biomarkers for predicting melanoma prognosis based on aging and immune microenvironment and therapeutic efficacy of immune checkpoints remain to be determined. Methods: The aging-related genes (ARGs) were obtained from the Human Ageing Genomic Resources and immune-related genes (IRGs) were downloaded from the Immunology database as well as Analysis Portal (ImmPort) database. Next, we initially performed LASSO regression and multivariate Cox regression to identify prognostic ARGs and IRGs in the TCGA and GSE65904 datasets, and firstly constructed a novel comprehensive index of aging and immune (CIAI) signature. Finally, in vitro molecular biology experiments were performed to assess the regulatory role of CNTFR in melanoma cell lines proliferation and migration, macrophage recruitment, and M2 polarization. Results: This novel CIAI signature consisted of 7 genes, including FOXM1, TP63, ARNTL, KIR2DL4, CCL8, SEMA6A, and CNTFR, in which melanoma patients in the high-CIAI group had shorter OS, DSS, and PFI, indicating CIAI model served as an independent prognostic index. Moreover, we found the CIAI score was potentially correlated with immune scores, estimate score, immune cell infiltration level, tumor microenvironment, immunotherapy effect, and drug sensitivity. Finally, CNTFR might function as oncogenes in melanoma cell lines and the silencing of CNTFR reduced macrophage recruitment and M2 polarization. Conclusion: In this study, we have first presented a novel prognostic CIAI model applied to assess immune checkpoint therapy and the efficacy of conventional chemotherapy agents in melanoma patients. Thus providing a new insight for combating melanoma.

Socioeconomic Disparities in Disability-Free Life Expectancy and Life Expectancy Among Older Chinese Adults From a 7-Year Prospective Cohort Study.

Objectives: We examined the magnitude and determinants of socioeconomic disparities in disability-free life expectancy and life expectancy at age 65 (DFLE65 and LE65) in China. Methods: Data from Chinese Longitudinal Healthy Longevity Survey collected during 2011-2018 (8,184 participants aged ≥65) were used. Socioeconomic status (SES) was measured by economic status (ES), and education, respectively. Multistate Markov models and microsimulations were fitted to estimate DFLE65 and LE65. Results: LE65 between high- and low-ES groups differed by 2.20 years for males and 2.04 years for females. The DFLE65 disparity in ES was 1.51 and 1.29 years for males and females, respectively. Not undergoing physical examinations, inadequate fruit/vegetable intake, and stress contributed to 35.10% and 57.36% of DFLE65 disparity in ES, as well as 26.36% and 42.65% of LE65 disparity for males and females, respectively. These disparities in education and ES were of a similar magnitude, while the above factors contributed little to education disparity. Conclusion: Socioeconomic disparities in DFLE65 and LE65 existed in China. Physical examination, fruit/vegetable intake and stress partly explained these disparities.

Light- and Field-Controlled Diffusion, Ejection, Flow and Collection of Liquid at a Nanoporous Liquid Crystal Membrane.

Liquid manipulation at solid surfaces has attracted plenty of interest yet most of them are limited to one or two direction(s), while transport in three dimensions is largely unexplored. Here, we demonstrate three-dimensionally steered dynamic liquid mobility at nanoporous liquid crystal polymer coatings. To this end, we orchestrate liquid motion via sequential triggers of light and/or electric field. Upon a primary flood exposure to UV light, liquid is ejected globally over the entire coating surfaces. We further reallocate the secreted liquid by applying a secondary electric field stimulus. By doing so, the liquid is transported and collected at pre-set positions as determined by the electrode positions. We further monitor this process in real-time and perform precise analysis. Interestingly, when applying those two triggers simultaneously, we discover a UV-gated liquid-release effect, which decreases threshold voltage as well as threshold frequency.

Improvement in Mung Bean Peptide on High-Fat Diet-Induced Insulin Resistance Mice Using Untargeted Serum Metabolomics.

This study aimed to elucidate the potential regulatory mechanism of mung bean peptides (MBPs) on glucolipid metabolism in insulin-resistant mice induced by high-fat diet (HFD) using untargeted serum metabolomics, enzyme linked immunosorbent assay (ELISA), intraperitoneal injection glucose tolerance test (IPGTT), insulin tolerance test (IPITT), and hematoxylin-eosin staining (H&E). The regulatory effect of MBPs for alleviating insulin resistance was studied by measuring body weight, fasting blood glucose (FBG) and serum insulin levels, C-Peptide levels, inflammatory and antioxidant factors, and histopathological observation of C57BL/6 mice. The experimental results showed that dietary intervention with MBPs (245 mg/kg/d) for 5 weeks significantly relieved insulin resistance in HFD mice. The body weight, insulin resistance index, and the levels of FBG, C-Peptide, IL-6, TNF-α, and MDA in the serum of HFD mice significantly decreased (P < 0.05). Conversely, SOD content and pancreatic ß cell function index significantly increased (P < 0.05), and the damaged pancreatic tissue was repaired. One biomarker associated with insulin resistance was glycine. In addition, there were four important differential metabolites: pyroglutamate, D-glutamine, aminoadipic acid, and nicotinamide, involved in 12 metabolic pathway changes. It was found that MBPs may regulate amino acid, glycerol phospholipid, fatty acid, alkaloid, and nicotinamide metabolism to regulate the metabolic profile of HFD mice in a beneficial direction.

Active Surfaces Formed in Liquid Crystal Polymer Networks.

There is an increasing interest in animating materials to develop dynamic surfaces. These dynamic surfaces can be utilized for advanced applications, including switchable wetting, friction, and lubrication. Dynamic surfaces can also improve existing technologies, for example, by integrating self-cleaning surfaces on solar cells. In this Spotlight on Applications, we describe our most recent advances in liquid crystal polymer network (LCN) dynamic surfaces, focusing on substrate-based topographies and dynamic porous networks. We discuss our latest insights in the mechanisms of deformation with the "free volume" principle. We illustrate the scope of LCN technology through various examples of photo-/electropatterning, free-volume channeling, oscillating/programmable network distortion, and porous LCNs. Finally, we close by discussing prominent applications of LCNs and their outlook.

Artificial Organic Skin Wets Its Surface by Field-Induced Liquid Secretion.

Living organisms enhance their survival rate by excreting fluids at their surface, but man-made materials can also benefit from liquid secretion from a solid surface. Known approaches to secrete a liquid from solids are limited to passive release driven by diffusion, surface tension, or pressure. Remotely triggered release would give active control over surface properties but is still exceptional. Here, we report on an artificial skin that secretes functional fluids by means of radiofrequency electrical signals driven by dielectric liquid transport in a (sub-)microporous smectic liquid crystal network. The smectic order of the polymer network and its director determine the flow direction and enhance fluid transport toward the surface at pre-set positions. The released fluid can be reabsorbed by the skin using capillary filling. The fluid-active skins open avenues for robotic handling of chemicals and medicines, controlling tribology and fluid-supported surface cleaning.

Liquid crystal polymers with motile surfaces.

In analogy with developments in soft robotics it is anticipated that soft robotic functions at surfaces of objects may have a large impact on human life with respect to comfort, health, medical care and energy. In this review, we demonstrate the possibilities and versatilities of liquid crystal networks and elastomers being explored for soft robotics, with an emphasis on motile surface properties, such as topographical dynamics. Typically the surfaces reversibly transfer from a flat state to a pre-designed corrugated state under various stimuli. But also reversible conversion between different corrugated states is feasible. Generally, the driving triggers are heat, light, electricity or contact with pH changing media. Also, the macroscopic effects of those dynamic topographies, such as altering the friction, wettability and/or performing work are illustrated. The review concludes with the existing challenges as well as outlook opportunities.

Dual-responsive deformation of a crosslinked liquid crystal polymer film with complex molecular alignment.

Crosslinked liquid crystal polymers (CLCPs) containing azobenzene mesogens have been developed as stimuli-responsive materials, which can undergo photodeformation and thus convert light energy into mechanical force. The deformation behavior of CLCPs is strongly influenced by the alignment of the mesogens; however, a precise control of the alignment domain at micro-scale is still a challenge. Here we report complex molecular alignment in the CLCP film by using photoalignment technology. First, azo dye SD1 is aligned in-plane by UV light with a discrete alternating striped director profile. The SD1 molecules in adjacent strips are aligned orthogonal, and the widths of the strips are controlled in several hundred micrometers by a photomask with grating patterns. Then the liquid crystal molecules in the CLCP film are aligned by SD1 through the anchoring effect on one side (SD1 side), and aligned perpendicular by the polyimide (PI) alignment layer on the other side (PI side). With these alignments, two kinds of splayed structures are formed through the depth of the film. When irradiated by UV light, the film bends toward the SD1 side with the bending direction along the diagonal of the film, determined by the resultant direction of molecular alignment on the SD1 side. When irradiated by blue light and heat, the bending direction is along the edge of the film. This dual-responsive deformable film with complex alignment is anticipated to be used in shape-changing biomedical devices, multiple controllable switches, and microactuators.

Protective effects of seed melon extract on CCl4-induced hepatic fibrosis in mice.

ETHNOPHARMACOLOGICAL RELEVANCE: Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao, was also known as watermelon belongs to family Cucurbitaceae, variously used as healthy food and in the treatment of liver and lungs problems. Currently, Citrullus lanatus has become a major economic crop of medicinal and edible effects with regional characteristics. AIM: This study was designed to evaluate the hepatoprotective and antioxidant activity of the seed melon (Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao) extract (SME) against carbon tetrachloride (CCl4) induced hepatic fibrosis in mice. MATERIALS AND METHODS: In this study, mice were randomly divided into 7 groups, including normal control, model, silymarin tablets as the positive control, SME 100, 200, 400, and 800mg/kg. After 8 weeks, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), hyaluronic acid (HA) and laminin (LN) were checked. The levels of antioxidant enzymes such as superoxide dismutase (SOD), glutataion (GSH) and glutathione peroxidase (GSH-Px) were determined after SME administration. The hydroxyproline (HYP) levels, malondialdehyde (MDA) levels and histopathologic examinations of hepatocyte fibrosis were also determined. Additionally, effects of SME on alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1(TGF-ß1) protein expressions were determined. RESULTS: We found that SME could significantly lower the serum levels of hepatic enzyme markers AST, ALT, HA and LN (P<0.01). Compared with the CCl4-only treatment group, levels of hepatic SOD and GSH-Px were significantly increased, and the MDA levels were remarkably decreased in mice treated by SME at medium dose (400mg/kg) and high dose (800mg/kg) (P<0.01). A histological examination of the liver showed that lesions, including necrosis, lymphocyte infiltration and fatty degeneration, were partially healed by treatment with SME. The results of protein expressions studies displayed that SME could inhibit α-SMA and TGF-ß1 protein expression (P<0.01). CONCLUSION: The present results suggested that protective effect of SME against CCl4-induced hepatic fibrosis may rely on its effect on reducing oxidative stress and improving drug metabolizing enzyme activity in liver.

Biomimetic Submicroarrayed Cross-Linked Liquid Crystal Polymer Films with Different Wettability via Colloidal Lithography.

Photoresponsive cross-linked liquid crystal polymer (CLCP) films with different surface topographies, submicropillar arrays, and submicrocone arrays were fabricated through colloidal lithography technique by modulating different types of etching masks. The prepared submicropillar arrays were uniform with an average pillar diameter of 250 nm and the cone bottom diameter of the submicrocone arrays was about 400 nm, which are much smaller than previously reported CLCP micropillars. More interestingly, these two species of films with the same chemical structure represented completely different wetting behavior of water adhesion and mimicked rose petal and lotus leaf, respectively. Both the submicropillar arrayed film and the submicrocone arrayed film exhibited superhyrophobicity with a water contact angle (CA) value of 144.0 ± 1.7° and 156.4 ± 1.2°, respectively. Meanwhile, the former demonstrated a very high sliding angle (SA) greater than 90°, and thus, the water droplet was pinned on the surface as rose petal. On the contrary, the SA of the submicrocone arrayed CLCP film consisting of micro- and nanostructure was only 3.1 ± 2.0°, which is as low as that of lotus leaf. Furthermore, the change on the wettability of the films was also investigated under alternating irradiation of visible light with two different wavelengths, blue light and green light.