ABSTRACT
Immunotherapies for cancers may cause severe and life-threatening cardiotoxicities. The underlying mechanisms are complex and largely elusive. Currently, there are several ongoing clinical trials based on the use of activated invariant natural killer T (iNKT) cells. The potential cardiotoxicity commonly associated with this particular immunotherapy has yet been carefully evaluated. The present study aims to determine the effect of activated iNKT cells on normal and ß-adrenergic agonist (isoproterenol, ISO)-stimulated hearts. Mice were treated with iNKT stimulants, α-galactosylceramide (αGC) or its analog OCH, respectively, to determine their effect on ISO-induced cardiac injury. We showed that administration of αGC (activating both T helper type 1 (Th1)- and T helper type 2 (Th2)-liked iNKT cells) significantly accelerated the progressive cardiac injury, leading to enhanced cardiac hypertrophy and cardiac fibrosis with prominent increases in collagen deposition and TGF-ß1, IL-6, and alpha smooth muscle actin expression. In contrast to αGC, OCH (mainly activating Th2-liked iNKT cells) significantly attenuated the progression of cardiac injury and cardiac inflammation induced by repeated infusion of ISO. Flow cytometry analysis revealed that αGC promoted inflammatory macrophage infiltration in the heart, while OCH was able to restrain the infiltration. In vitro coculture of αGC- or OCH-pretreated primary peritoneal macrophages with primary cardiac fibroblasts confirmed the profibrotic effect of αGC and the antifibrotic effect of OCH. Our results demonstrate that activating both Th1- and Th2-liked iNKT cells is cardiotoxic, while activating Th2-liked iNKT cells is likely cardiac protective, which has implied key differences among subpopulations of iNKT cells in their response to cardiac pathological stimulation.
Subject(s)
Cardiomegaly/etiology , Cardiotonic Agents/therapeutic use , Galactosylceramides/adverse effects , Glycolipids/therapeutic use , Lymphocyte Activation/drug effects , Natural Killer T-Cells/drug effects , Animals , Cardiomegaly/chemically induced , Cardiomegaly/drug therapy , Cardiomegaly/pathology , Cytokines/metabolism , Fibrosis , Inflammation/prevention & control , Isoproterenol , Macrophages/drug effects , Male , Mice, Inbred C57BL , Natural Killer T-Cells/classificationABSTRACT
Gq-protein is located at the convergent point in signal transduction pathways leading to vascular remodeling. The carboxyl terminus of Gα-subunit plays a vital role in G-protein-receptor interaction. The present study was designed to explore the effects of a synthetic Gαq carboxyl terminus imitation peptide, namely GCIP-27, on vascular smooth muscle cells (VSMC) in vitro and vascular remodeling in spontaneous hypertensive rats (SHR). Hyperplasia and hypertrophy of VSMC wre determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, [(3)H]-thymidine and [(3)H]-leucine incorporation, and [Ca(2+)](i) was measured with Fluo-3/AM staining. Systolic blood pressure (SBP), the ratio of media thickness to lumen diameter (MT/LD) of aorta, collagen content, and phospholipase C activity in aorta were measured in SHR. GCIP-27 (3-100 µg/l) significantly decreased proliferation activity, protein content, incorporation of [(3)H]-thymidine and [(3)H]-leucine, and [Ca(2+)](i) level in VSMC. SBP, MT/LD, collagen content, and phospholipase C activity in aorta of SHR were decreased significantly in GCIP-27 (7, 20, 60 µg/kg)-treated groups and losartan (6 mg/kg) group compared with vehicle group. In conclusion, GCIP-27 could inhibit vascular remodeling effectively in vitro and in vivo.
Subject(s)
Aorta/pathology , Cardiotonic Agents/pharmacology , Cell Proliferation/drug effects , GTP-Binding Protein alpha Subunits, Gq-G11/therapeutic use , Hypertension/pathology , Hypertrophy/pathology , Muscle, Smooth, Vascular/pathology , Myocytes, Smooth Muscle/pathology , Peptides/pharmacology , 3T3 Cells , Angiotensin II/pharmacology , Angiotensin II Type 1 Receptor Blockers/pharmacology , Animals , Aorta/cytology , Aorta/metabolism , Aorta/physiopathology , Blood Pressure/drug effects , Blood Pressure/physiology , Blood Pressure Determination , Calcium/analysis , Cardiotonic Agents/therapeutic use , Cell Culture Techniques , Collagen/metabolism , Dose-Response Relationship, Drug , Drug Evaluation, Preclinical , GTP-Binding Protein alpha Subunits, Gq-G11/physiology , Hypertension/drug therapy , Hypertension/metabolism , Hypertension/physiopathology , Hypertrophy/physiopathology , Losartan/pharmacology , Male , Mice , Molecular Targeted Therapy , Muscle, Smooth, Vascular/cytology , Muscle, Smooth, Vascular/drug effects , Muscle, Smooth, Vascular/metabolism , Myocytes, Smooth Muscle/cytology , Myocytes, Smooth Muscle/metabolism , Random Allocation , Rats , Rats, Inbred SHR , Rats, Inbred WKY , Rats, Wistar , Tetrazolium Salts , Thiazoles , Type C Phospholipases/metabolism , Vasoconstrictor Agents/pharmacologyABSTRACT
Prenatal exposure to inflammation produces offspring that are hypertensive in adulthood. The present study was to explore the role of intrarenal renin-angiotensin (Ang) system in the development of hypertension programmed by prenatal exposure to zymosan. Pregnant rats were randomly divided into control group and zymosan group (n = 6). Rats in these two groups were administered intraperitoneally with 0.5 ml vehicle and 2.37 mg/kg zymosan, respectively, on the eighth, tenth, and 12th day during gestation. The results showed the glomerular number and creatinine clearance rate decreased significantly in offspring of zymosan-treated rats. The renal cortex renin mRNA expression, Ang II-positive cells in renal cortex, and Ang II expression in renal medulla increased significantly in offspring of zymosan-treated rats at 7, 16, and 25 weeks of age. The plasma renin activity and Ang II concentration were unchanged. In conclusion, prenatal exposure to zymosan resulted in the activation of intrarenal renin-Ang system in adult offspring rats.
Subject(s)
Hypertension/physiopathology , Inflammation/physiopathology , Kidney Glomerulus/physiopathology , Prenatal Exposure Delayed Effects , Renin-Angiotensin System/physiology , Angiotensin II/blood , Angiotensin II/genetics , Animals , Blood Pressure , Creatinine/metabolism , Female , Gene Expression , Hypertension/blood , Hypertension/metabolism , Inflammation/pathology , Kidney Function Tests , Kidney Glomerulus/pathology , Polymerase Chain Reaction , Pregnancy , RNA, Messenger/genetics , Random Allocation , Rats , Rats, Sprague-Dawley , Renin/blood , Renin/genetics , Renin/metabolism , Zymosan/immunologyABSTRACT
1. Various G(q)-protein-coupled receptors, such as alpha(1)-adrenoceptors, angiotension AT(1) receptors, endothelin ET(A) receptors, neuropeptide Y(1) receptors etc., contribute to cardiac hypertrophy. In G-protein signalling pathways, the carboxyl terminus of the G(alpha) subunit plays a vital role within G-protein-receptor interaction. The present study was designed to explore the effects of the synthetic G(alphaq) carboxyl terminal imitation peptide GCIP-27 on cardiac hypertrophy. 2. Hypertrophy of rat cultured cardiomyocytes was induced by noradrenaline (NA) or angiotensin (Ang) II in vitro. Protein content, [(3)H] incorporation and [Ca(2+)](i) were determined in cardiomyocytes cultured with GCIP-27. Three in vivo animal models of cardiac hypertrophy were prepared using intraperitoneal injections of NA in mice and rats and suprarenal abdominal aortic stenosis in rats. After treatment with GCIP-27 (10-100 microg/L) for 15 or 20 days, indices of cardiac hypertrophy were measured. The effect of GCIP-27 on the mRNA expression of c-fos and c-jun was detected using reverse transcription-polymerase chain reaction. 3. At 10-100 microg/L, GCIP-27 significantly decreased protein content and [(3)H]-leucine incorporation in cultured cardiomyocytes compared with 1 micromol/L NA- and 1 micromol/L AngII-treated groups. After treatment with GCIP-27 (10, 30 or 100 microg/kg) for 15 days, the heart index (HI) and left ventricular index (LVI) in mice decreased significantly compared with the NA control group. In rats, GCIP-27 significantly reduced HI and LVI compared with the NA and aortic stenosis groups. Moreover, [Ca(2+)](i) in cardiomyocytes in the GCIP-27 (3, 10, 30 microg/L)-treated groups was lower than that in the control groups. Expression of c-fos and c-jun mRNA decreased significantly in the myocardium from 5-45 microg/L GCIP-27-treated rats compared with NA controls. 4. The results indicate that GCIP-27 can attenuate cardiac hypertrophy effectively in various models in vitro and in vivo.
Subject(s)
Cardiomegaly/drug therapy , GTP-Binding Proteins/pharmacology , Peptides/pharmacology , Animals , Calcium/metabolism , Cardiomegaly/chemically induced , Cardiomegaly/metabolism , Cells, Cultured , Genes, fos/genetics , Genes, jun/genetics , Leucine/metabolism , Male , Mice , Myocardium/metabolism , Myocytes, Cardiac/drug effects , Myocytes, Cardiac/metabolism , Norepinephrine , RNA, Messenger/metabolism , Rats , Rats, Sprague-DawleyABSTRACT
AIM: To investigate the effects of prenatal exposure to lipopolysaccharide (LPS) on blood pressure and body weight of offspring in rats. METHODS: Sixteen healthy, pregnant rats were randomly divided into 2 groups. The rats in the LPS group were injected intraperitoneally with LPS (0.79 mg/kg) on the d 8, d 10, and d 12 of gestation. Those in the control group were only treated with normal saline. After delivery, all offspring were weighed and blood pressure was measured by the tailcuff method once every 2 weeks from the 6th to the 24th week. In the 15th week, their food intake was weighed every day. At the end of the 24th week, the rats were killed by decapitation. Abdominal adipose tissues were weighed, and the serum level of leptin was detected by radioimmunoassay. RESULTS: The offspring with prenatal LPS exposure showed increased systemic arterial pressure, heavier body weight, elevated food intake, increased adipose tissue weight, and increased circulating leptin compared with the controls. CONCLUSION: Prenatal exposure to LPS leads to increases in blood pressure and body weight in rats.
Subject(s)
Blood Pressure/drug effects , Body Weight/drug effects , Fetus/drug effects , Lipopolysaccharides/pharmacology , Prenatal Exposure Delayed Effects , Adipose Tissue/anatomy & histology , Animals , Eating , Female , Fetus/physiology , Humans , Hypertension/etiology , Leptin/blood , Male , Pregnancy , Random Allocation , Rats , Rats, Sprague-DawleyABSTRACT
AIM: To clone and express G protein competitive inhibitory polypeptide (GCIP) gene and investigate the prophylactic effects of GCIP on myocardial hypertrophy in vitro. METHODS: The pIVEX2.3MCS-GCIP plasmid expressing GCIP was constructed by inserting double-stranded oligonucleotide into pIVEX2.3-MCS plasmid vector. Recombinant plasmid was expressed in Rapid Translation System 500 (RTS500). The expression of GCIP was identified by SDS-PAGE and Western blotting. The purification of GCIP with 6xHis tag was carried out on a Ni-NTA agarose column. The prophylactic effects of GCIP was observed on cardiomyocytes isolated from newborn Wistar rats. Protein synthesis rate were assessed by [3H]Leu incorporation. Protein contents were measured by Lowry method. RESULTS: The plasmid pIVEX2.3MCS-GCIP was successfully constructed. The expression of GCIP was about 2.43 % of total protein. GCIP was purified on Ni-NTA agarose column. The purity of the purified GCIP peptide was about 98 %. The contents of total protein and the rate of [3H]Leu incorporation were significantly decreased in (100 microg/L, 1 mg/L, and 10 mg/L) GCIP-treated groups in myocardial cellular hypertrophy model (P<0.01). CONCLUSION: The pIVEX2.3MCS-GCIP expression vector has been constructed successfully. The GCIP was expressed in RTS500 system and was purified with Ni-NTA agarose. GCIP was able to inhibit myocardial hypertrophy concentration-dependently in vitro.