Monolayer acellular dermal matrix for reconstruction of face burn: A case report.

Introduction: Facial burns constitute a severe medical and psychological challenge, dramatically affecting patients' quality of life. We present an innovative approach involving the use of a monolayer acellular matrix-specifically the INTEGRA® Dermal Regeneration Template Single Layer-to optimize skin grafting outcomes in a facial burn patient. Case report: The case revolves around a 45-year-old woman suffering a facial burn due to a clothes iron accident. Following escharectomy, dermabrasion, and homologous tissue graft placement, a monolayer acellular dermal matrix was strategically applied to the right malar area. Subsequently, we conducted a reconstruction with partial-thickness grafts. The integration of grafts with the acellular dermal matrix was seamless, absent of complications. The patient's healing process was marked by significant improvement, devoid of infections, bleeding, or any need for graft resection. Discussion: This case underscores the profound benefits of using a monolayer dermal matrix in facial burn reconstruction. Despite necessitating surgical expertise and meticulous wound preparation, this approach substantially reduced operating room time and improved clinical outcomes. This study illuminates the potential of acellular dermal matrix application in facial burn reconstruction, paving the way for further research and clinical advancements in this area.

Recubrimiento radicular de recesiones gingivales con matriz dérmica acelular / Root coverage of gingival recession with acellular dermal matrix

La recesión gingival es considerada como una deformidad o condición mucogingival, la Academia Americana de Periodontología, define a la recesión gingival como el desplazamiento del margen del tejido blando apical a la unión cemento-esmalte con la exposición de la superficie radicular. El tratamiento de las recesiones gingivales es un motivo de consulta común debido a razones estéticas, hipersensibilidad dentinaria, molestias durante el cepillado e incluso temor a la pérdida dentaria. Es una situación clínica común, 60% de la población humana tiene algún tipo de recesión gingival. Al realizar el examen clínico a paciente masculino de 55 años, se observó una recesión gingival tipo 1 (RT1) sin pérdida de inserción interproximal de la clasificación de Cairo. Se realizó el colgajo posicionado coronalmente (CPC) utilizando una matriz dérmica acelular (MDA) de origen humano OrACELL®. Se obtuvo resultado favorable en el recubrimiento de recesiones gingivales múltiples; considerándolos como una buena alternativa frente a los injertos gingivales autógenos. Concluyendo que, el uso de la matriz dérmica acelular para el tratamiento de la recesión gingival tipo 1 es una adecuada opción para el recubrimiento radicular. Se recomiendan más estudios a largo plazo para ver la estabilidad de los resultados obtenidos con la MDA (AU)

Gingival recession, considered a deformity or mucogingival condition, the American Academy of Periodontology, defines gingival recession as the exposure of the root surface resulting from migration of the gingival margin apical to the cementoenamel junction (CEJ). The treatment of gingival recessions is a common reason for consultation due to aesthetic reasons, dentin hypersensitivity, discomfort during brushing and even fear of tooth loss. It is a common clinical situation, 60% of the human population has some kind of gingival recession. Clinical examination of a 55-year-old male patient showed a type 1 gingival recession (RT1) without loss of interproximal insertion of the Cairo classification. Coronally advanced flap (CAF) was performed using an acellular dermal matrix (ADM) of human origin OrACELL®. Favorable results were obtained in the coating of multiple gingival recessions; considering them as a good alternative to autogenous gingival grafts. Concluding that, the use of the acellular dermal matrix for the treatment of gingival recession type 1, is a suitable option for root lining. Further long-term studies are recommended to see the elasticity of MDA outcomes (AU)

Current state about root coverage using soft-tissue substitutes in the presence of noncarious cervical lesions: A literature review.

About half of the cases of gingival recession are associated with the noncarious cervical lesion (NCCL), resulting in combined defects (CDs). NCCL negatively affects the root coverage outcomes. In addition, considering the morbidity associated with graft harvesting, soft-tissue substitutes (STSs) appeared as a suitable option for connective tissue grafts for surgical root coverage. Currently, the literature addressing the therapy of CDs employing STSs is scarce. Thus, the present review aimed to update the literature and outline the future perspectives about root coverage of CDs using STSs. A detailed literature search was conducted on MEDLINE, Web of Science, EMBASE, LILACS, Scopus, and Google Scholar databases using keywords and Boolean operators. Randomized clinical trials (2) and case reports (6) were included. None of the selected studies reported any adverse effect using STSs. Based on the limited evidence available, we cannot state that STSs may benefit the periodontal clinical and patient-centered outcomes. Randomized controlled trials are needed to assess the long-term outcomes, surgical approaches, and restorative protocols.

In-Vivo Evaluation of a Reinforced Ovine Biologic for Plastic and Reconstructive Procedures in a Non-human Primate Model of Soft Tissue Repair.

Background: Biologic matrices are used in plastic and reconstructive surgical procedures to aid in the kinetics of soft tissue repair and promote functional tissue formation. The human acellular dermal matrix AlloDerm is widely used; however, it is offered at a relatively high cost, and its dermal composition may not provide an ideal remodeling scaffold. OviTex Plastic and Reconstructive Surgery (PRS) Resorbable and Permanent are reinforced biologic matrices engineered with layers of ovine forestomach matrix embroidered with small amounts of polymer to optimize biophysical performance. This study compared the healing outcomes of these matrices in a non-human primate model of soft tissue repair. Methods: Animals were implanted with test articles in surgically created full-thickness midline abdominal wall defects and evaluated macroscopically and histologically at 2, 4, 12, and 24 weeks. Results: Both OviTex PRS Permanent and Resorbable matrices exhibited earlier host cell infiltration, neovascularization, and collagen deposition and also fully remodeled into the host tissue by 12 weeks post implantation. AlloDerm had less host cell infiltration and neovascularization at early time points and never fully integrated into the surrounding host tissue. There was no statistical difference in overall inflammation between AlloDerm and either OviTex PRS product at any time point, despite small amounts of polymer reinforcement in OviTex products. Conclusions: In a primate soft tissue repair model, OviTex PRS Permanent and Resorbable matrices performed comparably with the leading human acellular dermal matrix. OviTex PRS Permanent and Resorbable are less expensive than alternatives like AlloDerm and may promote faster host cell proliferation and functional remodeling in some soft tissue repair applications.

Tratamiento de recesiones gingivales tipo I y II de cairo: Reporte de caso / Treatment of cairo type I and II gingival recessions: Case report

Introducción: Las recesiones gingivales, son defectos mucogingivales que ocurren con mayor frecuencia en adultos y pueden aumentar con la edad. Existe una exposición parcial de la superficie radicular y puede causar problemas estéticos, funcionales y periodontales. Reporte de caso: Paciente femenino de 51 años de edad, sistémicamente sana, diagnosticada con recesiones tipo I y II de Cairo, las cuales se trataron con técnicas de colgajo posicionado coronal, túnel y con uso de biomateriales como matriz dérmica acelular y proteínas derivadas del esmalte. Conclusión: La importancia de tomar en cuenta el diagnóstico de la recesión, así como las características del defecto y tejidos adyacentes determinarán el éxito en el tratamiento.

Introduction: Gingival recessions are mucogingival defects that occur more often in adults and may increase with age. There is a partial exposure of the tooth root which can create aesthetic, functional and periodontal problems. Case Report: 51 year-old female patient, systematically healthy, was diagnosed with Cairo type I and II recessions, which were treated with techniques such as: coronally advanced flap, tunnel and with the use of biomaterials like acellular dermal matrix and enamel matrix derivatives. Conclusion: The importance of taking into account the diagnosis of the recession, as well as the characteristics of the defect and adjacent tissues, will determine the success of the treatment.

Integra Dermal Regeneration Template in reconstructive surgery for cutaneous tumours: a two-year retrospective review.

OBJECTIVE: Integra Dermal Regeneration Template (IDRT) (Integra LifeSciences, US) is a bioengineered dermal matrix that has been widely used in burn reconstruction since its first description. However, little is reported on its use in oncologic dermatological defects. Our objective was to evaluate reconstruction using IDRT on cutaneous tumour defects. METHOD: We conducted a two-year retrospective review of patients with skin tumours who had an excision surgery, followed by reconstruction with IDRT, as a mid-step towards a final autograft procedure: a split-thickness skin graft. The records of all patients at a single academic institution were queried from the electronic medical record using data obtained from the operating surgeon. RESULTS: We identified 13 patients with different tumour types and locations. The mean defect size was 105.92cm². The matrix take rate was 92.3% and average postoperative day for definite autograft was 20 days. Patients were followed for a period of up to 12 months. Of the patients, one had exposed bone without periosteum; another patient showed recurrence six months after matrix placement, requiring a new second two-stage IDRT-autograft procedure before radiation therapy. Patients reported complete satisfaction with the cosmetic, functional and oncological results. No cases of infection were encountered. CONCLUSION: IDRT is a valid option for the reconstruction of oncologic surgical defects of the skin and can be used in different anatomical locations. Specifically, it is an alternative to the reconstructive ladder when grafts and local flaps are not possible in those patients, and an option for patients who will eventually need adjuvant radiotherapy.

Reconstrução do antebraço com matriz dérmica acelular após excisão de neurofibroma plexiforme gigante: relato de casos / Forearm reconstruction with acellular dermal matrix after giant plexiform neurofibroma excision: case reports

Introdução: O neurinoma plexiforme gigante é um neuroectoderma e uma doença hereditária. É um tumor cutâneo incomum associado à NF1, caracterizado como um tumor benigno da bainha do nervo periférico envolvendo múltiplos fascículos nervosos. Os objetivos da reconstrução da cobertura do antebraço são proteger as estruturas que vão até o punho e a mão e evitar cicatrizes que levem à perda de movimento. Tanto o antebraço quanto a mão desempenham papéis funcionais e sociais. O manejo bem-sucedido de feridas complexas é necessário para a reabilitação funcional geral desses pacientes. Relato do Caso: Paciente do sexo feminino, 31 anos, apresentou-se na divisão de cirurgia plástica com neurofibroma plexiforme gigante no antebraço direito. Após ressecção cuidadosa, todos os tendões anteriores do antebraço foram expostos. O defeito foi coberto com Pelnac T enxertável (espessura de 3mm e tamanho 12 X 24cm2), fixados com pontos monocryl 4-0. Após 10 dias, a matriz dérmica acelular foi removida e um enxerto de malha de pele de espessura parcial foi colocado. No dia 7, a matriz dérmica acelular apresentou bons sinais de ingestão. No dia 17, observamos uma sobrevida do enxerto de 95%. No seguimento de 3 meses, a reconstrução estava estável, sem defeitos de contorno, a mão apresentava amplitude de movimento completa e o paciente não apresentava problemas nas atividades diárias. Conclusões: A matriz dérmica acelular parece ser uma opção útil na cobertura de defeitos complexos no antebraço, permitindo menor morbidade e rápida recuperação funcional.

Introduction: Giant plexiform neurinoma is a neuroectoderm and inherited disease. It is an uncommon skin tumor associated with NF1, characterized as a benign peripheral nerve sheath tumor surrounding multiple nervous fascicles. The goals of forearm coverage reconstruction are to protect the structures running to the wrist and hand and prevent scarring that leads to movement loss. Both forearm and hand play functional and social roles. Successful management of complex wounds is necessary for the overall functional rehabilitation of these patients. Case Report: A 31-year-old woman presented at the plastic surgery division with a giant plexiform neurofibroma in the right forearm. After careful resection, all anterior forearm tendons were exposed. The defect was covered with graftable Pelnac T (thickness of 3mm and sizing 12 X 24cm2), fixed with 4-0 monocryl sutures. After 10 days, the acellular dermal matrix silicone layer was removed, and a split-thickness skin meshed graft was placed. On day 7, the acellular dermal matrix showed good signs of intake. On day 17, we observed a 95% graft survival. At the 3-month follow-up, reconstruction was stable without contouring defects, the hand had full range of motion, and the patient had no problems in daily activities. Conclusions: Acellular dermal matrix appears to be a useful option in covering complex defects in the forearm, allowing for less morbidity and rapid functional recovery.

Root coverage with tunneling technique or modified advanced flap associated with acellular dermal matrix: results from 6 months randomized clinical trial.

OBJECTIVES: This study compared two surgical techniques using acellular dermal matrix (ADM) for the treatment of multiple gingival recessions. MATERIAL AND METHODS: Twenty patients, with bilateral RT1 gingival recessions, were selected. One side received a modified extended coronally advanced flap (MECAF), and the contralateral side a supra-periosteal flap (TUN). The evaluated parameters were probing depth (PD), relative clinical attachment level, gingival index, gingival recession height (GR), width of keratinized tissue, keratinized tissue thickness, and gingival recession area at baseline and 6 months postoperative. Pain was evaluated weekly, using a visual analog scale (VAS) during first month postoperative. RESULTS: Both groups were effective in reducing GR (ΔGR: MECAF 2.28 mm; TUN 1.93 mm), without significant differences. The % of root coverage was numerically superior favoring MECAF (MECAF 61.24%; TUN 56.07%), without significant differences between groups. VAS scale failed to provide differences between groups. CONCLUSIONS: Both treatment were effective in root coverage and might be valuable for the treatment of multiple gingival recessions. The use of vertical releasing incisions and a flap design including the papillae did not hamper root coverage. CLINICAL RELEVANCE: This study compared two techniques for use of ADM in large multiple gingival recessions. Within its limitations, both treatments successfully achieved root coverage and were able to reduce gingival recession. It is expected a partial root coverage when using these techniques in large gingival recessions. The study failed to provide significant differences between groups. The use of modified extended coronally advanced flap may be advisable for the treatment of multiple gingival recessions, specially involving large defects when using ADM, to avoid early matrix exposure.

Root coverage of gingival recessions with non-carious cervical lesions: a controlled clinical trial.

OBJECTIVE: The non-carious cervical lesion (NCCL) is commonly produced by improper toothbrushing techniques, occlusion trauma, anatomic mal-positioned teeth, and acid erosion, thus sharing the same etiology of gingival recession (GR). The association of a graft to the coronally advanced flap had demonstrated the best long-term outcome for root coverage (RC). However, substitutes for the autogenous graft must be studied. This split-mouth clinical trial investigates the RC and the increase in keratinized tissue (KT) when comparing RC of NCCLs associated with GR with intact roots using an extended coronally advanced flap (ECAF) associated with the acellular dermal matrix graft (ADMG), a connective tissue replacement graft. MATERIAL AND METHODS: Seventeen individuals with bilateral GR were included in the study. One side had a NCCL (TG) and the opposite root was intact (CG). All patients were treated with the ECDF associated with ADMG. All clinical parameters were assessed at baseline and 6 months postoperative. RESULTS: Root coverage means (CG, 69.5 ± 19 and TG. 72.2 ± 16.5; p value = 0.849570) were not significantly different between control and test groups. In addition, the KT had an increase in the follow-up period for both groups. CONCLUSION: GR associated with NCCLs can be successfully treated with the ECDF and ADMG. CLINICAL RELEVANCE: Patients frequently search for GR treatment due to cervical wear, root sensitivity, and compromising aesthetics. The NCCL participates with the same issues. The present study contributes to the literature that GR associated with NCCLs can be successfully treated with the ECAF and the ADMG.

Invited Discussion on: Skin Reducing Mastectomy and Prepectoral Breast Reconstruction in Large Ptotic Breasts.

The applicability of acellular dermal matrix (ADM) for breast reconstruction is a consolidated reality, as skin and nipple sparing techniques became standard mastectomy approaches. ADM is a soft connective tissue graft generated via a decellularization process that preserves intact the extracellular skin matrix. ADM not only provides tissue reinforcement, but also better pocket control, and shape without the compressive effects of total sub-muscular coverage. Our preference is using one "Strattice®" ADM in pocket's format to cover the implant's inferior pole, protecting the totality of the implant in its inferior pole by the ADM. This technique besides its versatility is cheaper than other techniques presented. The success of ADM prepectoral breast reconstruction depends on three pillars: careful patient selection, flap perfusion and postoperative management. The challenge in large and/or ptotic breasts under the risk of large badly perfused flaps as well as of nipple, due to the nipple-furcules distance which can be handled with well-selected criteria as mentioned and safe management of nipple ascension as well as the nipple graft. Traditionally, they need to be associated with reducing mammoplasty techniques to achieve the expected aesthetic results in a single stage. Therefore, the plastic surgeon must be prepared for new reconstructive approaches postmastectomy, having the ADM as an excellent option for that.

Root coverage stability with acellular dermal matrix in multiple gingival recessions in esthetic zone: A clinical case report with 12-year follow-up.

The importance of surgical intervention for the maintenance of long-term results by root coverage of multiple gingival recessions in an esthetic area treated with a tissue substitute (acellular dermal matrix [ADM]-Alloderm®) is widely required. The present case report highlights the effectiveness of the ADM for the treatment of multiple recession defects in a female patient with Class I and II gingival recession in relation to maxillary anterior associated with esthetics and dentin hypersensitivity demands. The root coverage rate for the anterior area showed greater value with 70% of coverage; at 90 days and 2 and 12 years of follow-up, it showed 70.5%, 79%, and 77%, respectively. Conversely, for the posterior area, these rates were 68.5%, 63%, and 57% for the same follow-up periods. Results regarding gain of keratinized tissue demonstrated superior values for the anterior area, namely 3.92, 3.34 and 3.22 mm at 90 days and 2 and 12 years. These values for the posterior area were 0.54 mm, 2.41, and 1.87 mm, respectively. These findings suggest that the tissue substitute Alloderm® showed excellent long-term results for areas of multiple recessions, providing root coverage and stability of keratinized tissue gain. After this long period of evaluation, some local factors associated with recurrence of gingival recessions were detected, and a lack of proper periodontal maintenance care was observed. However, esthetic and functional outcomes achieved with surgical procedure were maintained.

Long-Term Outcome (10 Years) of Multiple Recession-Type Defects Treated With Acellular Dermal Matrix Graft and Coronally Advanced Flap: A Case Report.

INTRODUCTION: There is limited evidence related to the long-term treatment outcomes of acellular dermal matrix graft (ADMG) plus coronally advanced flap (CAF) for the treatment of multiple recession defects. The aim of this case report is to assess the short- and long-term clinical outcomes of Miller Class I multiple gingival recession-type defects in the maxilla treated with ADMG plus CAF. CASE PRESENTATION: A 36-year-old female patient smoking < five cigarettes per day presented multiple adjacent Miller Class I gingival recession in the left maxilla and was referred for treatment with chief complaints of dental hypersensitivity and esthetics. Root coverage was achieved by ADMG + CAF and the patient underwent a 10-year follow-up. CONCLUSIONS: Within the limits of this case report, mean root coverage obtained at 6 months could not be maintained in the long term. A significant relapse was detected after 10 years, from 80% complete root coverage (CRC) at 6 months to 40% at 10 years. Monitoring, compliance, and reinstructions in oral hygiene techniques seem mandatory for minimizing relapse of gingival recessions after root coverage procedures.

Tratamiento Quirúrgico de la Recesión en el Sector Anterior a través de la Técnica de Túnel Modificada Utilizando Matriz Dérmica Acelular / Surgical Treatment of the Recession in the Anterior Sector through the Modified Tunnel Technique Using the Acellular Dermal Matrix

RESUMEN: Objetivo: El propósito del reporte de caso fue evaluar la cobertura radicular (CR) en el sector anterior luego de haber realizado la técnica de túnel modificada utilizando Matriz Dérmica Acelular (MDA). Consideraciones clínicas: La literatura ha descrito el uso de la técnica de túnel desde hace varias décadas y en los últimos años se ha perfeccionado con desplazamiento del tejido gingival hacia coronal simultáneo con el uso de MDA con el fin de lograr mejores resultados. Aunque en situaciones clínicas de cobertura de recesión gingival (RG) es discutible qué técnica puede ser más efectiva y existen diferentes alternativas, la técnica de túnel más colgajo desplazado coronal utilizando MDA es una línea de investigación prometedora y todavía no está concluída por tanto sería interesante profundizar al respecto. Conclusión: El caso clínico presenta el seguimiento de un paciente con recesión tipo 1 (RT1), que fue operado utilizando MDA y la técnica de túnel modificada demostrando que puede ser efectiva en la cobertura total de la recesión con resultados estéticos sostenibles en un período de 24 meses.

ABSTRACT: Objective: The purpose of the case report was to evaluate the radicular coverage (RC) in the upper anterior sector after having performed the modified tunnel technique using the Acellular Dermal Matrix (ADM). Clinical considerations: The literature has described the use of the tunnel technique for several decades and in recent years has been perfected with displacement of the gingival tissue to coronal with the use of ADM to achieve better results. Although in clinical situations of gingival recession (GR) coverage it is questionable which technique can be more effective and there are different alternatives, the tunneling technique plus coronal displacement flap using ADM is a promising line of research and is not yet concluded so it would be interesting to deepen in this regard. Conclusion: The clinical case presents the follow-up of a patient with recession type 1 (RT1) who was operated using ADM and the modified tunnel technique demonstrating that it can be effective in the total coverage of the recession with sustainable aesthetic results in a period of 24 months.

Yeasts from skin colonization are able to cross the acellular dermal matrix.

In recent decades, the prognosis for burn patients has improved considerably with the development of specialized care. The acellular dermal matrix (ADM) is a totally artificial acellular device that functions to control water loss, prevent penetration by bacteria and allow migration of endothelial cells and fibroblasts from patient tissues. However, little is known about its effectiveness against yeasts. The present study evaluated the capacity of colonization and migration of some human commensal yeasts. Three clinical isolates from skin scales, identified as Candida parapsilosis, Candida glabrata and Rhodotorula mucilaginosa, were used. Their ability to cross the ADM was evaluated. After three days, all isolates had crossed the ADM. C. parapsilosis showed the lowest growth, while R. mucilaginosa showed intermediate and C. glabrata the highest growth. In the plates incubated for seven days, the growth of C. parapsilosis and C. glabrata increased by 1 log over the third day. All isolates have the capacity to colonize and migrate through the matrix, increasing the potential risk to burn patients, who can develop severe and even fatal infections by invasive fungi.

Sustitutos dérmicos definidos / Dermal substitutes defined

Son muchas las patologías que producen defectos de piel. El método de elección para la cobertura cutánea de estos defectos es el injerto de piel parcial, sin embargo, en algunos casos sus resultados no son adecuados. Los sustitutos dérmicos son una alternativa de cobertura cutánea, que existen en la actualidad y que permiten obtener mejores resultados funcionales y estéticos. Existen muchos sustitutos dérmicos en el mercado, cada uno con distintas características, beneficios y complicaciones. Esta revisión logra agrupar los aspectos más relevantes de los sustitutos dérmicos para poder tener una base teórica sobre estos productos que son de gran ayuda para poder tratar a pacientes con distintas patologías.

Many are the pathologies that cause skin defects. The gold standard for skin coverage of these defects is the partial skin graft, but in some cases the results are not appropiate. Dermal substitutes are an alternative for skin coverage that exists today and allow get better functional and aesthetic results. There are many dermal substitutes in the pharmaceutical industry, each one with different characteristics, benefits and complications. This review brings together the most important aspects of dermal substitutes to have a theoretical background on these products that are helpful to treat patients with different pathologies.

Matriz dérmica acelular e conjuntivo gengival, no tratamento das recessões periodontais: avaliação clínica comparativa / Acellular dermal matrix and connective gingival in the treatment of periodontal recessions: comparative clinical evaluation in humans

Diferentes técnicas cirúrgicas periodontais têm sido propostas para o tratamento das recessões periodontais. Dentre essas, o enxerto de tecido conjuntivo gengival destaca-se pela alta previsibilidade de sucesso. Esse trabalho comparou os resultados clínicos do tratamento das recessões periodontais, utilizando os enxertos de tecido conjuntivo gengival e matriz dérmica acelular. Um total de 36 recessões constituiu a amostra, divididas igualmente em 2 grupos. O grupo 1 foi tratado com o enxerto de matriz dérmica acelular (MDA) associado ao retalho deslocado coronariamente, enquanto o grupo 2 (CG) recebeu o tratamento com enxerto de tecido conjuntivo gengival, também associado ao retalho deslocado coronariamente. Foram avaliados os parâmetros clínicos de profundidade de sondagem, nível clínico de inserção, recessão periodontal no sentido corono-apical, diâmetro mésio-distal das recessões, faixa de mucosa ceratinizada inserida e influência da espessura do retalho e dos enxertos em relação à cobertura radicular. As medidas iniciais foram comparadas às obtidas com 45, 90 e 120 dias pós-operatórios. Não foram encontradas diferenças estatisticamente significantes, entre os grupos, em termos de redução das recessões periodontais, faixa de mucosa ceratinizada inserida e espessura do retalho e dos enxertos em relação à cobertura radicular. O grupo 2 (CG) apresentou redução estatisticamente significante, na profundidade de sondagem e nível clínico de inserção, comparativamente ao grupo 1(MDA). Porém, essas diferenças não foram clinicamente relevantes. Concluiu-se que tanto o enxerto de tecido conjuntivo gengival quanto a matriz dérmica acelular podem ser utilizados no tratamento das recessões periodontais, com uma alta previsibilidade de sucesso.

Different techniques have been proposed for the treatment of periodontal recessions. This study compared the clinical results of periodontal recession treatment using a connective tissue graft and an acellular dermal matrix. A totalof 36 recessions were treated and assigned to the group one and the contralateral recession to the group two. In the group one, the exposed root surfaces were treated by the placement of a acellular dermal matrix in combination with a coronally positioned flap; in the group two, a connective tissue graft was used. Probing depth, clinical attachment level, periodontal recession, and width of Keratinized tissue were measured in the procediment initial and 45, 90 and 120 days post surgery. There were no statistically significant differences between the group one and the group two in terms of recession reduction and width of Keratinized tissue. The group two (connective tissue graft) had a statiscally significant reduction in probing depth and clinical attachment gain, so these differences were no clinical significants. Based on these results, the connective tissue graft and acellular dermal matrix can be used for the treatment of periodontal recession, with a high success preview.

Evaluation of in vitro human gingival fibroblast seeding on acellular dermal matrix

The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donor's tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90 percent of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.

A matriz dérmica acelular (MDA) foi introduzida na Periodontia como um substituto para enxertos autógenos, os quais se tornaram restritos devido à quantidade limitada de tecido doador. O objetivo deste estudo foi verificar, in vitro, a distribuição, proliferação e viabilidade de fibroblastos gengivais humanos cultivados em MDA. Fibroblastos gengivais foram cultivados sobre MDA por até 21 dias. Os seguintes parâmetros foram avaliados: distribuição, proliferação e viabilidade celular. Os resultados revelaram que, aos 7 dias, os fibroblastos estavam aderidos e espraiados na superfície da MDA, e estavam distribuídos de forma desigual, formando uma camada celular descontínua; aos 14 dias, uma monocamada confluente de fibroblastos revestindo a superfície da MDA foi observada. Aos 21 dias, a monocamada celular exibiu uma redução na densidade celular. Aos 7 dias, cerca de 90 por cento das células aderidas na superfície da MDA estavam no ciclo celular, enquanto que aos 14 e 21 dias esse número reduziu significativamente. Uma maior proporção de células viáveis foi detectada na superfície da MDA tanto aos 14 quanto aos 21 dias. Os resultados sugerem que fibroblastos cultivados sobre a MDA por 14 dias permitem boas condições de adesão e espraiamento das células sobre a matriz, porém, a migração de células para o interior da matriz foi limitada.

Avaliação clínica comparativa da sintomatologia após a realização de enxertos gengivais livres autógenos e alógenos para a criação e manutenção de uma faixa de mucosa ceratinizada / Comparative clinical evaluation of postoperative morbidity after autogenous free gingival graft and dermal matrix allograft to gain and maintain keratinized tissue

Avaliou-se comparativamente os sinais e sintomas pós-operatórios de enxertos gengivais livres (EGL) e de matriz dérmica acelular (MDA) em 22 pacientes que possuíam duas áreas com quantidade inadequada de mucosa ceratinizada na região de pré-molares inferiores de arcos homólogos, sendo que cada paciente foi submetido aos dois tipos de enxertos. A análise foi realizada por meio de respostas a um questionário de sinais e sintomas no pós-operatório imediato de 10 dias. Os resultados foram analisados estatisticamente pelo teste de McNemar, p < 0,05, para comparar as frequências relativas de cada ocorrência (dor, edema e sangramento) e também para analisar o sítio de escolha do paciente de maior sensibilidade dolorosa, demonstrando similaridade de sintomatologia nos sítios enxertados e eleição da área doadora de enxerto autógeno como a mais sensível. Com base nesses resultados pode-se concluir que a MDA quando utilizada como substituta dos EGL apresenta menor desconforto no pós-operatório por não necessitar de uma área cirúrgica adicional para remoção de tecido.

This clinical study compared the postoperative morbidity resulting of free gingival graft and acellular dermal matrix allografts. Twenty two patients with contralateral insufficient keratinized tissue were submitted to both grafts and answered a signals and symptoms questionnaire in the 10th immediate postoperative day. McNemar test, p < 0,05, was used to compare the relative frequency of pain, swelling, bleeding and to analyze the most sensible area in accordance with the patients. The postoperative sensibility of the receptor area was comparable in both groups and the donor area of free gingival graft was the most sensible. The results of this study suggest that acellular dermal matrix allografts can be used as free gingival graft substitute with less postoperative discomfort and it’s not necessary any additional site to remove autogenous tissue.

Análise histológica da utilização da matriz dérmica acelular e da matriz de pericárdio bovino, associados ao retalho reposicionado coronariamente, no tratamento de deiscências ósseas criadas cirurgicamente em cães / Acellular dermal matrix and bovine pericardium matrix in treatment of deiscence-type defects. A histomorphometric study in dogs

O propósito deste estudo foi avaliar histológica e histometricamente o resultado da utilização das matrizes de colágeno de Pericárdio Bovino (MPB) e Dérmica Acelular (MAD) no tratamento de deiscências ósseas criadas cirurgicamente em cães. Foram criados defeitos periodontais do tipo deiscência óssea na superfície vestibular dos 2os, 3os e 4os prémolares inferiores de seis cães. Estes dentes foram divididos em 3 grupos: grupo I: MAD; grupo II: controle e grupo III: MPB, ambos com reposição coronária do retalho. Após 70 dias os animais foram sacrificados, os dentes retirados em bloco e processados histologicamente. Realizou-se análise histológica descritiva e histométrica, medindo-se a extensão do tecido epitelial, neoformação óssea e cementária e nível gengival para os grupos I, II e III. Os resultados demonstraram valores estatisticamente iguais para as medianas (em mm) dos valores de epitélio: 1,49, 1,91 e 1,82; cemento: 3,18, 3,09 e 3,31 respectivamente para os grupos I, II e III. Foi encontrada diferença estatisticamente significante em relação à neoformação óssea, com medianas (em mm) de 1,23, 1,82 e 1,62, respectivamente para os grupos I, II e III. Foi observado escasso infiltrado inflamatório. Com isso, pudemos concluir que as matrizes de MPB e MAD são materiais que permitem a reparação dos tecidos periodontais

The goal of this investigation was to compare, histologically and histometrically, the healing process of dehiscence-type defects treated by acellular dermal matrix (AD) and bovine pericardium matrix (PB). Six mongrel dogs were used. Buccal osseous deiscences were surgically created on mesial roots of the mandibular second, third and fourth premolars. The defects were randomly assigned to one of the treatments: AD, control and PB. After 70 days of healing, the dogs were sacrificed and the blocks were processed. The histometric parameters evaluated included: gingival level, epithelial length, new cementum and new bone. No statistically significant differences were found between PB, AD and control groups for epithelial length and new cementum parameters (p<0,05). Statistically significant differences were found between PB, AD and control groups for new bone (minor for AD). The presence of a slight inflammation was observed. Withim the limits of this study, it can be concluded that PB and AD showed good biocompatibility, but did not bring a complete periodontal tissue regeneration