ABSTRACT
Manganese(IV) (Mn(IV)) reduction coupled to anaerobic ammonium (NH4+-N) oxidation (Mnammox) is a recently identified metal oxide-mediated nitrogen (N) loss pathway, holding potential value for the efficient removal of NH4+-N from wastewater. However, little is known about the application of Mnammox in wastewater treatment. Here, a novel Mnammox bacterium Aromatoleum evansii (strain MAY27) was screened. Strain MAY27 can utilize MnO2 as an electron acceptor to achieve NH4+-N removal under a low C/N condition (C/N = 0.5). The influencing factors in the Mnammox process and the Mn(IV) reduction driving effect on NH4+-N oxidation were investigated. The physiological characteristics of strain MAY27 and differential metabolic pathways were identified through whole-genome sequencing and metabolomic analyses. A significant up-regulation of several key pathways upon the addition of MnO2, including glycolysis/gluconeogenesis, transmembrane transporter activity, and oxidoreductase activity. This study contributes to the advancement of biotechnological approaches for treating N-containing wastewater.
ABSTRACT
Constructed wetlands (CWs) have emerged as effective wastewater treatment systems, mimicked natural wetland processes but engineered for enhanced pollutant removal efficiency. Ammonium (NH4+) and nitrate (NO3-) are among common pollutants in wastewater, posing significant environmental and health risks. The primary objective of this study is to compares the performance of CWs using gravel and three sizes of natural pumice, along with phragmites australis, in horizontal and horizontal-vertical CWs for nitrate and ammonium removal in the complementary treatment of domestic wastewater. Additionally, the study aims to develop and validate a numerical model using MATLAB software to predict the removal efficiency of these pollutants, thereby contributing to the optimization of CW design and operation. The model operates as a zero-dimensional model based on the law of mass conservation, treating the wetland as a completely mixed reactor, thus avoiding complexities associated with solute movement in porous media. It accurately could predict removal efficiency of chemical, biochemical, and biological indicators while considering active and passive absorption mechanisms by plant uptake. Notably, the determination of coefficients in the model equation does not rely on potentially error-prone laboratory measurements due to sampling issues. Instead, optimization techniques alongside field data robustly estimate these coefficients, ensuring reliability and practicality. Results indicate that higher pollutant concentrations increase reaction rates, particularly enhancing CW efficiency in ammonium removal. Pumice, especially in larger sizes, exhibits superior absorption due to increased porosity and surface area. Overall, the model accurately predicts nitrates concentrations, demonstrating its potential for CW performance optimization and confirming the significance of effective pollutant removal strategies in wastewater treatment.
ABSTRACT
Microbial fuel cells (MFCs) show promise in sewage treatment because they can directly convert organic matter (OM) into electricity. This study aimed to demonstrate MFCs stability over 750 days of operation and efficient removal of OM and nitrogenous compounds from sewage. To enhance contaminant removal, oxygen was provided into the anode chamber via a mini air pump. This pump was powered by the MFCs' output voltage, which was boosted using a DC-DC converter. The experimental system consisted of 12 sets of cylindrical MFCs within a 246L-scale reactor. The boosted voltage reached 4.7 V. This voltage was first collected in capacitors every 5 min and then dispensed intermittently to the air pump for the MFCs reactor in 4 s. This corresponds to receiving average DO concentration reaching 0.34 ± 0.44 mg/L at 10 cm above the air-stone. Consequently, the degradation rate constants (k) for chemical oxygen demand (COD) and biological oxygen demand (BOD) in the presence of oxygen were 0.048 and 0.069, respectively, which surpassed those without oxygen by 0.039 and 0.044, respectively. Aeration also marginally improved the removal of ammonia because of its potential to create a favorable environment for the growth of anammox and ammonia-oxidizing bacteria such as Candidatus brocadia and Nitrospira. The findings of this study offer in-depth insight into the benefits of boosted voltage in MFCs, highlighting its potential to enhance contaminant degradation. This serves as a foundation for future research focused on improving MFCs performance, particularly for the removal of contaminants from wastewater.
ABSTRACT
Algae-mediated nitrogen removal from low carbon vs. nitrogen (C/N) wastewater techniques has garnered significant attention due to its superior autotrophic assimilation properties. This study investigated the ammonium-N removal potential of four algae species from low C/N synthetic wastewater. Results showed that 95 % and 99 % of ammonium-N are eliminated at initial concentrations of 11.05 ± 0.98 mg/L and 42.51 ± 2.20 mg/L with little nitrate and nitrite accumulation. The compositions of secreted algal-derived dissolved organic matter varied as C/N decreased and showed better bioavailability for nitrate-N removal by Pseudomonas sp. SZF15 without pre-oxidation, achieving an efficiency of 99 %. High-throughput sequencing revealed that the aquatic microbial communities, dominated by Scenedesmus, Kalenjinia, and Micractinium, remain relatively stable across different C/N, aligning with the underlying metabolic pathways. These findings may provide valuable insights into the sustainable elimination of multiple nitrogen contaminants from low C/N wastewater.
Subject(s)
Denitrification , Nitrogen , Wastewater , Wastewater/chemistry , Biodegradation, Environmental , Nitrates/metabolism , Ammonium Compounds/metabolism , Water Purification/methods , Water Pollutants, Chemical/metabolism , Carbon , Organic ChemicalsABSTRACT
This study investigates the potential of humic substances (HS) and graphene oxide (GO), as extracellular electron acceptors (EEA) for nitrification, aiming to explore alternatives to sustain this process in wastewater treatment systems. Experimental results demonstrate the conversion of ammonium to nitrate (up to 87 % of conversion) coupled to the reduction of either HS or GO by anaerobic consortia. Electron balance confirmed the contribution of HS and GO to ammonium oxidation. Tracer analysis in incubations performed with 15NH4+ demonstrated 15NO3- as the main product with a minor fraction ending as 29N2. Phylogenetic analysis identified Firmicutes, Euryarchaeota, and Chloroflexi as the microbial lineages potentially involved in anoxic nitrification linked to HS reduction. This study introduces a new avenue for research in which carbon-based materials with electron-accepting capacity may support the anoxic oxidation of ammonium, for instance in bioelectrochemical systems in which carbon-based anodes could support this novel process.
Subject(s)
Carbon , Nitrification , Carbon/chemistry , Electrons , Graphite/chemistry , Phylogeny , Oxidation-Reduction , Ammonium Compounds/metabolism , Anaerobiosis , Nitrates/metabolism , Bacteria/metabolismABSTRACT
Industrial, e.g. food industrial and domestic wastewaters contain huge amount of compounds causing eutrophication, and should be removed with high cost during wastewater treatment. However, these compounds could be utilized as fertilizers too. Biochar can remove a wide range of pollutants from water, such as ammonium, which can be found in relatively high concentration in dairy wastewaters. However, adsorption performance may be affected by the presence of other wastewater pollutants. Thus, this study aims to determine the efficiency of biochar as an adsorbent of ammonium in aqueous solutions in the presence of some selected organic compounds of typical dairy wastewaters such as bovine serum albumin (BSA), lactose, and acetic acid. Methods: The biochar was produced from banana leaves at 300 °C, modified with NaOH, and characterized by Scanning Electron Microscope - Energy Dispersive X-Ray Spectroscopy (SEM-EDX), Fourier-transform infrared spectra (FTIR) analysis, and specific surface area measurements. Batch experiments were carried out to investigate the ammonium adsorption capacity and the ion competitive adsorption mechanism. Significant Findings: Results show that the surface structure of the biochar derived from banana leaves is different from other biochars previously studied; although the specific surface area is not very considerable and despite having nitrogen within the elemental composition, the biochar studied is capable of adsorbing 2.60 mg NH4+/m2, the highest ammonium removal in 2 h occurs at pH 9 and 500 mg biochar dose. Langmuir model in the monolayer phase analysis fits better for all scenarios and the maximum NH4+ adsorption capacity was 0.97 mg/g without organic compounds. In the multilayer adsorption phase, the isotherm model that best fits the data obtained is the Harkins-Jura model without organic compounds. The presence of organic compounds in the aqueous solution significantly impacts the adsorption of ammonium by biochar since it improves the adsorption capacity (1.132 mg/g BSA, 0.975 mg/g lactose, and 1.874 mg/g acetic acid). The Aranovich-Donohue isotherm model fitted the data obtained during ion competitive adsorption experiments well.
ABSTRACT
In this study, a sulfonation approach using chlorosulfonic acid (CSA) to prepare cellulose sulfate nanofibers (CSNFs) from raw jute fibers is demonstrated. Both elemental sulfur content and zeta potential in the CSNFs are found to increase with increasing CSA content used. However, the corresponding crystallinity in the CSNFs decreases with the increasing amount of CSA used due to degradation of cellulose chains under harsh acidic conditions. The ammonium adsorption results from the CSNFs with varying degrees of sulfonation were analyzed using the Langmuir isotherm model, and the analysis showed a very high maximum ammonium adsorption capacity (41.1 mg/g) under neutral pH, comparable to the best value from a synthetic hydrogel in the literature. The high ammonium adsorption capacity of the CSNFs was found to be maintained in a broad acidic range (pH = 2.5 to 6.5).
ABSTRACT
High Cl- concentration in saline wastewater (e.g., landfill leachate) limits wastewater purification. Catalytic Cl- conversion into reactive chlorine species (RCS) arises as a sustainable strategy, making the salinity profitable for efficient wastewater treatment. Herein, aiming to reveal the structure-property relationship in Cl- utilization, bismuth oxychloride (BiOCl) photocatalysts with coexposed {001} and {110} facets are synthesized. With an increasing {001} ratio, the RCS production efficiency increases from 75.64 to 96.89 µg L-1 min-1. Mechanism investigation demonstrates the fast release of lattice Cl- as an RCS and the compensation of ambient Cl-. Correlation analysis between the internal electric field (IEF, parallel to [001]) and normalized efficiency on {110} (kRCS/S{110}, perpendicular to [001]) displays a coefficient of 0.86, validating that the promoted carrier dynamics eventually affects Cl- conversion on the open layered structure. The BiOCl photocatalyst is well behaved in ammonium (NH4+-N) degradation ranging from 20 to 800 mg N L-1 with different chlorinity (3-12 g L-1 NaCl). The sustainable Cl- conversion into RCS also realizes 85.4% of NH4+-N removal in the treatment of realistic landfill leachate (662 mg of N L-1 NH4+-N). The structure-property relationship provides insights into the design of efficient catalysts for environment remediation using ambient Cl-.
Subject(s)
Ammonium Compounds , Bismuth , Water Pollutants, Chemical , Wastewater , Water Pollutants, Chemical/chemistry , SalinityABSTRACT
The formation of stable and mature biofilms affects the efficient and stable removal of ammonium by biological activated carbon (BAC). In this study, the new granular activated carbon (GAC) was preloaded with the carbon source (glucose and sucrose) and nano manganese dioxide (nMnO2) before using. Then tests were performed to determine whether substrate preloading promoted ammonium removal. The ammonium removal treated by nMnO2 coupled with sucrose-loaded BAC reached 49.1 ± 2.5%, which was 1.7 times higher than that by the nonloaded BAC 28.2 ± 1.9%). The biomass on the substrate-loaded BAC reached 5.83 × 106-1.22 × 107 cells/g DW GAC on Day 7, which was 4.6-9.5 times higher than the value of the nonloaded BAC (1.28 × 106 cells/g DW GAC). The amount of extracellular polymer (i.e., protein) on nMnO2 coupled to sucrose-loaded BAC was promoted significantly. Flavobacterium (0.7%-11%), Burkholderiaceae (13%-20%) and Aquabacterium (30%-67%) were the dominant functional bacteria on the substrate-loaded BAC, which were conducive to the nitrification or denitrification process. The results indicated that loading nMnO2 and/or a carbon source accelerated the formation of biofilms on BAC and ammonium removal. Additionally, the ammonium removal treated by nMnO2 coupled with sucrose-loaded BAC was contributed by microbial degradation (56.0 ± 2.5%), biofilm adsorption (38.7 ± 2.1%) and GAC adsorption (5.3 ± 0.3%), suggesting a major role of microbial degradation.
Subject(s)
Ammonium Compounds , Water Purification , Charcoal , Nitrification , Biofilms , Sucrose , Water Purification/methodsABSTRACT
In the context of the anaerobic ammonium oxidation process (anammox), great scientific advances have been made over the past two decades, making anammox a consolidated technology widely used worldwide for nitrogen removal from wastewaters. This review provides a detailed and comprehensive description of the anammox process, the microorganisms involved and their metabolism. In addition, recent research on the application of the anammox process with alternative electron acceptors is described, highlighting the biochemical reactions involved, its advantages and potential applications for specific wastewaters. An updated description is also given of studies reporting the ability of microorganisms to couple the anammox process to extracellular electron transfer to insoluble electron acceptors; particularly iron, carbon-based materials and electrodes in bioelectrochemical systems (BES). The latter, also referred to as anodic anammox, is a promising strategy to combine the ammonium removal from wastewater with bioelectricity production, which is discussed here in terms of its efficiency, economic feasibility, and energetic aspects. Therefore, the information provided in this review is relevant for future applications.
Subject(s)
Ammonium Compounds , Wastewater , Denitrification , Nitrogen/metabolism , Anaerobic Ammonia Oxidation , Electrons , Oxidation-Reduction , Anaerobiosis , Bioreactors , Ammonium Compounds/metabolism , OxidantsABSTRACT
The strategy of nitrogen sufficiency conversion can improve ammonium nitrogen (NH4+-N) removal with microalgal cells from ammonium-rich wastewater. We selected and identified one promising isolated algal strain, NCU-7, Chlorella sorokiniana, which showed a high algal yield and tolerance to ammonium in wastewater, as well as strong adaptability to N deprivation. The transition from N deprivation through mixotrophy (DN, M) to N sufficiency through autotrophy (SN, P) achieved the highest algal yields (optical density = 1.18 and 1.59) and NH4+-N removal rates (2.5 and 4.2 mg L-1 d-1) from synthetic wastewaters at two NH4+-N concentrations (160 and 320 mg L-1, respectively). Algal cells in DN, M culture obtained the lowest protein content (20.6%) but the highest lipid content (34.0%) among all cultures at the end of the stage 2. After transferring to stage 3, the lowest protein content gradually recovered to almost the same level as SN, P culture on the final day. Transmission electron microscopy and proteomics analysis demonstrated that algal cells had reduced intracellular protein content but accumulated lipids under N deprivation by regulating the reduction in synthesis of protein, carbohydrate, and chloroplast, while enhancing lipid synthesis. After transferring to N sufficiency, algal cells accelerated their growth by recovering protein synthesis, leading to excessive uptake of NH4+-N from wastewater. This study provides specific insights into a nitrogen sufficiency conversion strategy to enhance algal growth and NH4+-N removal/uptake during microalgae-based ammonium-rich wastewater treatment.
Subject(s)
Ammonium Compounds , Chlorella , Microalgae , Water Purification , Ammonium Compounds/metabolism , Wastewater , Chlorella/metabolism , Microalgae/metabolism , Nitrogen/metabolism , Biomass , LipidsABSTRACT
The urgent need to address the severe issue of nitrogen pollution has prompted the search for a functional and easy recycling material. In this study, manganese oxides (MnOx) were loaded on activated carbon (AC), resulting in a composite known as AC-MnOx, for efficient ammonium removal from aqueous solutions. The results indicated a remarkable 15.6-fold increase in ammonium removal efficiency and a fivefold enhancement in removal capacity for AC-MnOx (3.20 mg/g) compared to AC. Under specific conditions (initial NH4+-N concentration of 15 mg/L, adsorbent dose of 2.5 g, pH of 6.5, and temperature of 35 â), the highest achieved ammonium removal efficiency reached 94.6%. Furthermore, the study distinguishes the contributions of catalytic oxidation and adsorption in the removal process. The adsorption process was effectively modeled using pseudo-second-order kinetics and Langmuir isotherm models. Interestingly, the amount of oxidation conversion (Ntur) exhibited a linear relationship with the dosage when the initial ammonium concentration was sufficiently high, while the relationship between initial ammonium concentration and the ratio of Ntur to adsorption capacity (Nsur) followed a negative exponential trend. The removal mechanisms involved electrostatic interaction between ammonium and the negatively charged dehydrogenated hydroxyl groups (- OHsur) or cation tunnel in crystal structures of MnOx, ion exchange adsorption, and the oxidation impact of MnOx. This research provides valuable insights into the application of immobilized MnOx media for ammonium removal. Moreover, filling AC-MnOx into constructed wetlands (CW) proved to be an effective method for reducing ammonium pollution, demonstrating its potential in the field of engineering wastewater treatment.
Subject(s)
Ammonium Compounds , Water Pollutants, Chemical , Charcoal/chemistry , Wastewater , Adsorption , Ammonium Compounds/analysis , Oxides/chemistry , Manganese Compounds/chemistry , Kinetics , Hydrogen-Ion Concentration , Water Pollutants, Chemical/analysisABSTRACT
The release of ammonia (as NH4+) into water bodies causes serious environmental problems. Therefore, the removal of ammonia from wastewater effluents has become a worldwide concern. New autotrophic biological alternatives for ammonia removal could reduce the limitations of conventional organic carbon-dependent nitrification-denitrification methods. Here, the potential of anaerobic ammonium oxidation coupled to Fe3+ reduction (a process known as Feammox) is studied in wastewater treatment plants of the yeast and beer production industry, not related to ammonium or iron treatment. This process is presented as a viable option to improve the efficiency of ammonia removal from wastewater. The results of this study show that enrichments under Feammox conditions achieved removals of 28.19-32.25% of the total NH4+. The highest rates of ammonium removal and Fe3+ reduction were achieved using FeCl3 as iron source and pH = 7.0. Different environmental conditions for the enrichments were studied and it was found that the use of sodium acetate as a carbon source and an incubation temperature of 35 °C presented higher rates of iron reduction and higher increase in nitrate concentration, related to ammonium oxidative processes. Likewise, the presence of relevant species of the iron and nitrogen cycles as Ferrovum myxofaciens, Geobacter spp, Shewanella spp, Albidiferax ferrireducens and Anammox was verified, supporting the findings of this study. These results provide information that may be relevant to the potential applicability of Feammox to treat wastewater with high ammonia load and could help develop cost-effective and environmentally friendly methods for ammonium removal in wastewater treatment plants.
Subject(s)
Ammonium Compounds , Wastewater , Ammonia , Anaerobiosis , Nitrogen/analysis , Iron , Oxidation-Reduction , Bioreactors , DenitrificationABSTRACT
Diminishing phosphorus resources worldwide requires developing new technologies to recover phosphorus (P) from wastewaters. A lab-scale electrolytic reactor with a magnesium anode was investigated to remove NH4+ and PO43- from synthetic wastewater by producing struvite. The effects of mixing speed, pH, and applied current on struvite yield, NH4+, and PO43- removal efficiencies were first evaluated using a factorial design. Then, the two most significant parameters were further optimized using Central Composite Design (CCD) coupled with Response Surface Methodology (RSM). The struvite was characterized by SEM, XRD, and FT-IR. A 5.7-fold increase in struvite yield was achieved by increasing the applied current from 0.1 to 0.5 A. The three regression equations generated by the CCD/RSM design with applied current and mixing speed as the two independent parameters were highly correlated with the response variables (struvite yield, NH4+ and PO43- removal efficiencies). The desirability analysis showed the best operating condition: current, 0.5 A and mixing speed, 414 rpm, for the reactor system, under which the optimal struvite yield and NH4+ and PO43- removal efficiencies were 4.75 g/L, 93.0%, and 58.4%, respectively. The SEM, XRD, and FT-IR analyses confirmed the high purity and quality of the struvite produced by the electrolytic reactor system.
Subject(s)
Magnesium , Wastewater , Struvite/chemistry , Magnesium/chemistry , Spectroscopy, Fourier Transform Infrared , Phosphorus/chemistry , Electrodes , Phosphates/chemistryABSTRACT
Hydrogen-based membrane biofilm reactors (MBfRs) are effective for nitrogen removal. However, the safety of hydrogen limited the application of MBfR. Here, a hydrogen-based partial denitrification system coupled with anammox (H2-PDA) was constructed in an MBfR for reducing hydrogen demand significantly. The metabolomics and structures of microbial communities were analyzed to determine the phenotypic differences and drivers underlying denitrification, anammox, and H2-PDA. These findings indicated that total nitrogen (TN) removal increased from 57.1% in S1 to 93.7% in S2. During the H2-PDA process, partial denitrification and anammox contributed to TN removal by 93.7% and 6.3%, respectively. Community analysis indicated that the H2-PDA system was dominated by the genus Meiothermus, which is involved in partial denitrification. Collectively, these findings confirmed the feasibility of incorporating the H2-PDA process in a MBfR and form a foundation for the establishment of novel and practical methods for efficient nitrogen removal.
Subject(s)
Ammonium Compounds , Nitrates , Denitrification , Anaerobic Ammonia Oxidation , Bioreactors , Oxidation-Reduction , Anaerobiosis , Methane , Biofilms , NitrogenABSTRACT
Vertical up-flow-constructed wetlands integrating with microbial fuel cell (VFCW-MFC) were evaluated for NH4+-N removal and bioelectricity recovery. The experiments were carried out in lab-scale VFCW-MFC microcosms treating synthetic domestic wastewater under different operational conditions of pH, hydraulic retention time, and mass loading rate. Effects of wild ornamental grass (Cenchrus setaceus) on treatment performance and voltage output were investigated simultaneously. Experiments demonstrated that the neutral pH of influents favoured NH4+-N removal and power generation. Extended retention time improved treatment capacity and power output but likely depended on the substrate availability. COD removal and power output increased, while NH4+-N removal decreased with increasing mass loading rates. At the loading rate of 88.31 mg COD/L.day, planted VFCW-MFCs exhibited better NH4+-N treatment performance (36.9%) and higher voltage output (132%-143%) than unplanted systems. Plants did not affect the COD removal efficiency of VFCW-MFCs (>95%). Power density was in the range of 1.26-1.59 mW/m2 in planted microcosms with a maximum CE of 13.6%. The anode layer accounted for a major proportion of NH4+-N removal in VFCW-MFCs. This study implies that NH4+-N in domestic wastewaters with relatively high COD:N ratios can be treated effectively in up-flow CW-MFCs via anaerobic processes, including anammox and heterotrophic denitrifying processes. The mass loading rate could be a critical parameter to balance different microbial processes, thus, coincidently determining the potential of power recovery from wastewaters.
Subject(s)
Bioelectric Energy Sources , Wastewater , Wetlands , Electrodes , ElectricityABSTRACT
Volatile fatty acids (VFAs) produced during anaerobic digestion (AD) of organic waste are a promising alternative carbon source for various biological processes; however, their applications are limited due to the presence of impurities such as ammonium (NH4+). This study investigates the potential for removal of ammonium using a naturally occurring zeolite (clinoptilolite) from chicken manure (CKM) derived VFA effluent recovered from an anaerobic membrane bioreactor (MBR). Experiments were conducted for both synthetic and actual VFA (AD-VFA) solutions, and the effects of different parameters were investigated with batch and continuous studies. It was observed that the Langmuir-type isotherm provided the best fit to the equilibrium data in the isotherm investigations carried out with the AD-VFA solution. The maximum adsorption capacity (qm) was found as 15.7 mg NH4+/g clinoptilolite. The effect of some operational parameters on process performance such as pH, initial NH4+ loading and potassium ion (K+) concentration was investigated. The pH had a negligible effect on ammonium removal for a pH range of 3-7, while the removal efficiency of ammonium decreased with the increase of initial NH4+ loading and K+ concentration. At the optimum conditions determined in batch experiments, the ammonium removal from synthetic and AD-VFA solutions were compared and average ammonium removal efficiencies of 93 and 94% were found in 12 h equilibrium time for synthetic and AD-VFA solutions, respectively. Overall findings indicated that clinoptilolite has excellent potential for ion exchange when combined with biological processes such as acidogenic fermentation of VFAs to purify the solution from high-ammonium content.
Subject(s)
Ammonium Compounds , Zeolites , Anaerobiosis , Bioreactors , Carbon , Fatty Acids, Volatile , Hydrogen-Ion Concentration , Ion Exchange , Manure , Potassium , Zeolites/chemistryABSTRACT
This review updates the current research efforts on using BES to recover NH3/NH4+, highlighting the novel configurations and introducing the working principles and the applications of microbial fuel cell (MFC), microbial electrolysis cell (MEC), microbial desalination cell (MDC), and microbial electrosynthesis cell (MESC) for NH3/NH4+ removal/recovery. However, commonly studied BES processes for NH3/NH4+ removal/recovery are energy intensive with external aeration needed for NH3 stripping being the largest energy input. In such a process bipolar membranes used for yielding a local alkaline pool recovering NH3 is not cost-effective. This gives a chance to microbial electrosynthesis which turned out to be a potential alternative option to approach circular bioeconomy. Furtherly, the reactor volume and NH3/NH4+ removal/recovery efficiency has a weakly positive correlation, indicating that there might be other factors controlling the reactor performance that are yet to be investigated.
Subject(s)
Ammonium Compounds , Bioelectric Energy Sources , Alkanesulfonic Acids , Ammonia , WastewaterABSTRACT
Excess amount of nitrogen in wastewater has caused serious concerns, such as water eutrophication. Paracoccus pantotrophus MA3, a novel isolated strain of heterotrophic nitrification-anaerobic denitrification bacteria, was evaluated for nitrogen removal using formic acid as the sole carbon source. The results showed that the maximum ammonium removal efficiency was observed under the optimum conditions of 26.25 carbon to nitrogen ratio, 3.39% (v/v) inoculation amount, 34.64 °C temperature, and at 180 rpm shaking speed, respectively. In addition, quantitative real-time PCR technique analysis assured that the gene expression level of formate dehydrogenase, formate tetrahydrofolate ligase, 5,10-methylenetetrahydrofolate dehydrogenase, serine hydroxymethyltransferase, respiratory nitrate reductase beta subunit, L-glutamine synthetase, glutamate dehydrogenase, and glutamate synthase were up-regulated compared to the control group, and combined with nitrogen mass balance analysis to conclude that most of the ammonium was removed by assimilation. A small amount of nitrate and nearly no nitrite were accumulated during heterotrophic nitrification. MA3 exhibited significant denitrification potential under anaerobic conditions with a maximum nitrate removal rate of 4.39 mg/L/h, and the only gas produced was N2. Additionally, 11.50 ± 0.06 mg/L/h of NH4+-N removal rate from biogas slurry was achieved.
Subject(s)
Ammonium Compounds , Formate-Tetrahydrofolate Ligase , Paracoccus pantotrophus , Aerobiosis , Ammonium Compounds/metabolism , Anaerobiosis , Biofuels , Carbon , Denitrification , Formate Dehydrogenases/metabolism , Formate-Tetrahydrofolate Ligase/metabolism , Formates , Glutamate Dehydrogenase , Glutamate Synthase/metabolism , Glutamate-Ammonia Ligase/metabolism , Glycine Hydroxymethyltransferase/metabolism , Methylenetetrahydrofolate Dehydrogenase (NADP)/metabolism , Nitrate Reductase/metabolism , Nitrates/metabolism , Nitrification , Nitrogen/metabolism , Paracoccus pantotrophus/metabolism , Wastewater , WaterABSTRACT
Riverbanks play the key role in ammonium removal from runoff entering river. Currently, microplastics (MPs) are frequently detected in riverbanks receiving urban and agricultural runoff. Nevertheless, the effect of MPs accumulation on ammonium removal in riverbanks is still unknown. We utilized sediment flow-through reactors to investigate the impact and mechanism of MPs accumulation on ammonium removal in riverbanks. These results revealed that MPs accumulation decreased ammonium removal in sediment by 8.2 %-12.8 % resulting from the reduction in nitrifier abundance (Nitrososphaera and Nitrososphaeraceae) and genes encoding ammonium and hydroxylamine oxidation (amoA, amoB, amoC, and hao) by MPs accumulation. Furthermore, MPs accumulation decreased the substrate and gene abundance of hydroxylamine oxidation process to reduce N2O emission (16.3 %-34.3 %). Notably, mathematic model verified that sediment physical properties changed by MPs accumulation were direct factors affecting ammonium removal in riverbank. It was suggested that both the biotoxicity of MPs and sediment physical properties should be considered in the ammonium removal process. To summarize, this study for the first time comprehensively clarifies the impact of MPs on the ammonium removal capacity of riverbanks, and provides information for taking measures to protect the ecological function of the riverbank and river ecosystem from MPs and ammonium pollution.