Minimally invasive guided endodontic microsurgery with Zekrya bur: A 48-month follow-up case report.

Endodontic microsurgery guided by navigation systems represents a precise and minimally invasive approach for retreatment of apical periodontitis following failed conventional endodontic therapy. Accurate localization and minimal access to the root apex are paramount for successful outcomes and preservation of anatomical structures. Workflow considerations, such as three-dimensional (3D) virtual planning and endodontic guidance represent new crucial aspects for addressing complex cases. This clinical case report presents the successful reintervention of a complex case using a Zekrya bur, operative microscopy, cone beam computed tomography and biocompatible materials. After a 48-month follow-up, complete healing of the treated area was observed. This minimally invasive technique, employing a simple instrument widely available globally, underscores the potential for efficient and predictable outcomes in complex endodontic microsurgical retreatment.

In vitro antibacterial activity of green tea-loaded chitosan nanoparticles on caries-related microorganisms and dentin after Er:YAG laser caries removal.

This study aimed to determine the inhibitory effects of green tea (Gt), EGCG, and nanoformulations containing chitosan (Nchi) and chitosan+green tea (Nchi+Gt) against Streptococcus mutans and Lactobacillus casei. In addition, the antibacterial effect of nanoformulations was evaluated directly on dentin after the selective removal of carious lesion. At first, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against S. mutans and L. casei isolates were investigated. In parallel, dentin specimens were exposed to S. mutans to induce carious lesions. Soft dentin was selectively removed by Er:YAG laser (n=33) or bur (n=33). Remaining dentin was biomodified with Nchi (n=11) or Gt+Nchi (n=11). Control group (n=11) did not receive any treatment. Dentin scraps were collected at three time points. Microbiological analyses were conducted and evaluated by agar plate counts. Gt at 1:32 dilution inhibited S. mutans growth while 1:16 was efficient against L. casei. EGCG at 1:4 dilution completely inhibited S. mutans and L. casei growth. Independently of the association with Gt, Nchi completely inhibited S. mutans at 1:4 dilution. For L. casei, different concentrations of Nchi (1:32) and Nchi+Gt (1:8) were required to inhibit cell growth. After selective carious removal, viability of S. mutans decreased (p<0.001), without difference between bur and Er:YAG laser (p>0.05). Treatment with Nchi and Nchi+Gt did not influence the microbial load of S. mutans on dentin (p>0.05). Although variations in concentrations were noticed, all compounds showed antibacterial activity against S. mutans and L. casei. Both bur and Er:YAG laser have effectively removed soft dentin and reduced S. mutans counts. Nanoformulations did not promote any additional antibacterial effect in the remaining dentin.

Effect of green tea-loaded chitosan nanoparticles on leathery dentin microhardness.

The purpose of this study was to assess the effect of a chitosan-based nanoformulation containing green tea on leathery (remaining) dentin subsurface microhardness. Size distribution, polydispersity index (PDI) and zeta potential (mV) of nanoformulations were previously determined by dynamic light scattering (DLS). Human dentin specimens were exposed to Streptococcus mutans for 14 d. Soft dentin were selectively removed by Er:YAG laser (n = 30) or bur (n = 30). Remaining dentin was biomodified with chitosan nanoparticles (Nchi, n = 10) or green tea-loaded chitosan nanoparticles (Gt + Nchi, n = 10) for 1 min. Control group (n = 10) did not receive any treatment. Subsurface microhardness (Knoop) was evaluated in hard (sound) and soft dentin, and then, in leathery dentin and after its biomodification, at depths of 30, 60 and 90 µm from the surface. Nchi reached an average size of ≤ 300 nm, PDI varied between 0.311 and 0.422, and zeta potential around + 30 mV. Gt + Nchi reached an average size of ≤ 350 nm, PDI < 0.45, and zeta potential around + 40 mV. Soft dentin showed significantly reduced microhardness at all depths (p > 0.05). The subsurface microhardness was independent of choice of excavation method (p > 0.05). At 30 µm from the surface, Gt + Nchi increased the leathery dentin microhardness compared to untreated group (p < 0.05). Nchi promoted intermediate values (p > 0.05). Both nanoformulations showed an average size less than 350 nm with nanoparticles of different sizes and stability along the 90-day period evaluated. Subsurface microhardness of bur-treated and laser-irradiated dentin was similar. At 30 µm, the biomodification with Gt + Nchi improved the microhardness of leathery dentin, independently of caries excavation method used.

The influence of different surface treatment protocols and bonding agents on the repair microtensile bond strength of six-month' aged composite: An in vitro study / A influência de diferentes protocolos de tratamento de superfície e agentes de união na resistência de união à microtração de reparo de compósito envelhecido por seis meses: um estudo in vitro

Objective: To compare the effect of three surface treatment protocols and two intermediate agents on repairing aged composite, regarding microtensile bond strength (µTBS) and mode of fracture, at two time intervals. Material and methods: Six-monthaged microhybrid composite blocks, were randomly distributed into three groups, subjected to; Fine, Super Fine grit diamond burs or Erbium- Yag Laser surface treatment. Each block had both One Coat bond SL (Bond) and Brilliant Flow flowable composite (Flow) intermediate agents, alongside. Blocks were incrementally repaired using nanohybrid composite, cut into beams, then randomly subjected either immediately (IM) to µTBS test or after thermocycling (TC) for 5000 cycle. Mode of failure was determined using stereomicroscope. Data were analyzed through three-way ANOVA followed by pairwise comparison with Bonferroni correction. Kruskal Wallis test compared groups for failure mode analysis (α=0.05) Results: Super Fine grit showed the highest mean µTBScompared to control for both intermediate agents, IM and after TC at P<0.05. No difference between Fine grit and Laser application for all groups (P>0.05). IM, Bond showed the highest µTBS compared to TC, Flow for all tested groups. Beams roughened with Fine and Super Fine burs showed significantly lower adhesive failures than those roughened with Laser. Flow suffers significantly higher adhesive failure than those with Bond. For TC tested groups; beams with Super Fine bur and Bond showed significantly lower adhesive failure at P=0.029. Conclusion: Super Fine grit and Bond provide the highest µTBS and the least adhesive failure; moreover TC resulted in significant decrease in µTBS (AU)

Objetivo: Comparar o efeito de três protocolos de tratamento de superfície e dois agentes intermediários no reparo de compósito envelhecido, quanto à resistência à microtração (µTBS) e modo de fratura, em dois intervalos de tempo. Material e métodos: Blocos de compósito microhíbrido envelhecidos por seis meses, foram distribuídos aleatoriamente em três grupos, submetidos a; Pontas diamantadas de granulação Fina, Superfina ou tratamento de superfície com laser de Erbio-Yag. Cada bloco tinha ambos os agentes intermediários One Coat bond SL (Adesivo) ao lado de Brilliant Flow (Resina Fluida). Os blocos foram reparados de forma incremental usando compósito nanohíbrido, cortados em palitose, em seguida, submetidos aleatoriamente imediatamente (IM) ao teste de µTBS ou após termociclagem (TC) por 5000 ciclos. O modo de falha foi determinado usando estereomicroscópio. Os dados foram analisados por meio de ANOVA de três fatores, seguida de comparação pareada com correção de Bonferroni. O teste de Kruskal Wallis comparou os grupos para análise do modo de falha (α = 0,05). Resultados: a granulação Super fina apresentou a maior média de µTBS em comparação com o controle para ambos os agentes intermediários, IM e após TC (P <0,05). Não houve diferença entre granulação fina e aplicação de laser para todos os grupos (P> 0,05). IM, Adesivo apresentou a µTBS mais alta em comparação com TC, Resina Fluida para todos os grupos testados. Os palitos asperizados com pontas de granulações Fina e Superfina apresentaram falhas adesivas significativamente menores do que as asperizadas com Laser. Resina Fluida sofreu falhas adesivas significativamente maiores do que aquelas com Adesivo. Para grupos testados TC; palitos tratados com ponta Superfina e Adesivo apresentaram falha adesiva significativamente menor com P = 0,029. Conclusão: a granulação Superfina e o Adesivo fornecem a maior µTBS e o mínimo de falha adesiva; além disso, o TC resultou em uma diminuição significativa da µTBS. (AU)

Assessment of the Ability of Different Cleaning Protocols to Remove Eugenol-based Endodontic Sealer from the Root Dentin.

AIM: The aim of this study is to assess three protocols for removing a eugenol-based filling endodontic sealer from root dentin and the bond strength of fiberglass posts luted with resin cement. MATERIALS AND METHODS: Sixty single-rooted bovine teeth were prepared and filled with the Endofill endodontic sealer and gutta-percha cones. After 7 days, 9 mm of the filling was removed and divided into five groups (n = 12) according to the cleaning protocol: G1 (negative control), unfilled; G2 (positive control), saline solution; G3, 95% alcohol; G4, amyl acetate; and G5, Largo bur. The canals were hybridized and fiberglass posts were luted with resin cement. They were cross-sectioned in slices of 1 mm and subjected to the push-out test. The other samples were sectioned longitudinally and visualized in a scanning electron microscope (SEM), and the existing chemical elements were quantified by energy dispersive spectroscopy (EDS). The statistical analysis used variance analysis (ANOVA)-one way and Fisher's test, at a 5% significance level. RESULTS: The 95% alcohol was more effective for cleaning the canal, resulting in a clean dentinal surface and bond strength statistically similar to the negative control. The Largo bur was also statistically similar to the negative control, with only a few debris impregnated on the wall. Amyl acetate showed more sealer residues on the canal walls, with a consequent lower bond strength value than the other groups, only higher than the positive control. CONCLUSION: The 95% alcohol and Largo burs may be used after removing the canal filling, so that the bond strength is improved when using the eugenol-based sealer.

Cutting efficiency of different diamond burs after repeated cuts and sterilization cycles in autoclave.

CONTEXT: The aim was to evaluate the cutting efficiency of different diamond burs after successive cuts and repeated sterilization in an autoclave. The morphology and grit sizes were analyzed and correlated to cutting efficiency. MATERIALS AND METHODS: Ten medium-grit diamond burs of five different manufacturers were investigated (KG, KG Sorensen; TH, Tri-Hawk; KM, Komet; HC, Heico; and FD, Frank Dental). Changes in the cutting efficiency of diamond burs on composite resin blocks were measured after five repeated cuts and after five sterilization cycles. Grit sizes were analyzed by scanning electron microscopy (SEM) and correlated to cutting efficiency. The data were statistically analyzed using 3-way ANOVA and Tukey's test (α = 0.05). RESULTS: Significant differences were observed for diamond burs (P < 0.0001) and condition (P < 0.0001). FD presented the lowest mean cut time (21.88s), followed by KM (36.08s). TH (40.18s), HC (41.65s), and KG (42.23s) had the highest cut times. The number of cuts was not statistically significant. New burs had a significantly shorter cutting time (33.38s) when compared with the ones after sterilization cycles (39.55s). A moderate to strong positive correlation was found between diamond size and cutting time (Pearson's coefficient of 0.77). CONCLUSION: All diamond burs demonstrated lower cutting efficiency after repeated autoclaving. Cutting efficiency did not decrease as the number of cuts increased.

G2/M inhibitors as pharmacotherapeutic opportunities for glioblastoma: the old, the new, and the future.

Glioblastoma (GBM) is one of the deadliest tumors and has a median survival of 3 months if left untreated. Despite advances in rationally targeted pharmacological approaches, the clinical care of GBM remains palliative in intent. Since the majority of altered signaling cascades involved in cancer establishment and progression eventually affect cell cycle progression, an alternative approach for cancer therapy is to develop innovative compounds that block the activity of crucial molecules needed by tumor cells to complete cell division. In this context, we review promising ongoing and future strategies for GBM therapeutics aimed towards G2/M inhibition such as anti-microtubule agents and targeted therapy against G2/M regulators like cyclin-dependent kinases, Aurora inhibitors, PLK1, BUB, 1, and BUBR1, and survivin. Moreover, we also include investigational agents in the preclinical and early clinical settings. Although several drugs were shown to be gliotoxic, most of them have not yet entered therapeutic trials. The use of either single exposure or a combination with novel compounds may lead to treatment alternatives for GBM patients in the near future.

Study of the relationships between bur diameter and dental implant diameter / Estudio de las relaciones entre diámetro de fresas y diámetro de implantes dentales

The bur used to construct the bed where a dental implant is installed is extremely important to treatment success. The aim of this investigation was to analyze the relationship between bur diameter and implant diameter in different areas. A descriptive study was designed to analyze 5 commercial brands currently on the market. The manufacturer's instructions were followed to establish the size and type of bur indicated for the study implants, which were regular, with a width between 3.5 mm and 4.0 mm and a length from 12 mm to 13 mm. Drilling from the most apical region, the first measurement of width was established 3 mm cervically, the lower middle measurement 6 mm cervically, the upper middle measurement at 9 mm and the cervical measurement at 12 mm. A scanning electron microscope was used standardized for all measurements; the data were analyzed using the Shapiro-Wilk test and the t-test, having considering a value of p<0.05 for significant differences. At apical level, all the implants were wider than the bur. The greatest differences between bur width and implant were observed at cervical level, maintaining a relation where the implant was always wider. In medium-sized areas, it was observed that in some systems the burs have a larger diameter than the study implants. A significant relation was established in the apical, upper middle and cervical areas. Bur morphology is related to implant morphology; no implant system is the same as another in terms of the implant-bur relation.

La relación entre la fresa utilizada para construir el lecho donde se instala el implante dental tiene alta importancia en el éxito del tratamiento. El objetivo de esta investigación fue analizar la relación entre el diámetro de fresa y el diámetro de implantes en diferentes áreas. Se diseñó un estudio descriptivo analizando utilizando 5 marcas comerciales vigentes en el mercado; se siguió las instrucciones del fabricante para establecer el tamaño y tipo de fresa indicada para el implante en análisis, los cuales fueron de tipo regular considerando medidas en ancho de entre 3,5 mm y 4,0 mm y en largo de 12 mm a 13 mm. Desde la región mas apical de implantes y fresar se estableció 3 mm hacia cervical la primera medición de ancho, 6 mm hacia cervical la medición media inferior, a 9 mm la medición media superior y a 12 mm la medición cervical; para esta medición se utilizó microscopio electrónico de barrido estandarizado para todas las mediciones; los datos fueron analizados con pruebas estadísticas de Shapiro-Wilk y la prueba t-test, considerando un valor de p<0,05 para considerar diferencias significativas. A nivel apical, todos los implantes fueron mas anchos que las fresas utilizadas. A nivel cervical es donde se observan las mayores diferencias entre el ancho de la fresa y del implante manteniendo una relación donde el implante siempre es mas ancho. En áreas medianas, se observó que en algunos sistemas las fresas son de mayor diametro a los implantes analizados. Se estableció relación significativa en las regiones apical, media superior y cervical. La morfología de fresas presenta relación con la morfología implantaría; ningún sistema de implantes es igual a otro en términos de relación implante y fresa.

Scanning electronic microscopy analysis of the apical surface after of root-end resection with different methods.

To compare the time dispensed and the surface characteristics when a root-end resection was performed with a surgical ultrasonic device (VarioSurg, NSK, Japan) or with a Zekrya bur. Forty distal roots of freshly extracted mandibular molars were selected and divided into four different groups: (G1) stainless steel insert with ultrasound; (G2) diamond insert with ultrasound; (G3) stainless steel and diamond inserts with ultrasound, and (G4) Zekrya burs. The time required for the root-end resection was timed and the surface characteristics were evaluated via scanning electronic microscopy. The median times for the root-end resections were: 115.9 s for G1, 249.8 s for G2, 112.7 s for G3, and 7.44 s for G4, the latter presented a smoother root-end surface. There was no statistically significant difference in the comparisons between G3 group and G4 group (Zekrya burs). The other groups where the root-end resections were with ultrasonic devices proportioned a prolonged time to resection with irregular surfaces.

Evaluación de un nuevo método de remoción quirúrgica de implantes oseointegrados considerados en fallo / Evaluation of a new surgical removal method of failed integrated implants

OBJETIVO El objetivo del presente estudio descriptivo es la valoración de un nuevo método de remoción de implantes oseointegrados bajo una técnica mínimamente invasiva. MATERIALES Y MÉTODOS El estudio fue desarrollado sobre un grupo de 66 implantes oseointegrados de conexión interna y externa diagnosticados en falla, a los cuales se les indicó su remoción quirúrgica por presentar un diagnóstico radiográfico de pérdida ósea periimplantaria, mal posicionamiento quirúrgico con imposibilidad de rehabilitación protésica o daño del elemento de retención protésico del implante. El procedimiento quirúrgico, el cual es de carácter ambulatorio, fue llevado a cabo en un pabellón bajo anestesia local. Como primera opción de remoción quirúrgica, el procedimiento consistió en la utilización de instrumental de remoción a contra-torque, sin la apertura de un colgajo de espesor total, y como segunda opción de remoción quirúrgica la utilización de una fresa trefina, para lo cual fue necesario realizar un colgajo de espesor total que expusiera el lecho implantario. RESULTADOS Se removieron un total de 66 implantes oseointegrados, todos bajo la primera modalidad de remoción a contra-torque con el nuevo instrumento, sin colgajo y sin la necesidad de usar fresa trefina. CONCLUSIONES Con las limitaciones de este estudio preliminar planteamos que la utilización del nuevo instrumento de remoción a contra-torque de implantes oseointegrados en fallo se vislumbra como una muy buena alternativa de remoción quirúrgica mínimamente invasiva, disminuyendo los riesgos quirúrgicos del uso de una fresa trefina, el daño a los tejidos aledaños.

OBJECTIVE The aim of this study is the evaluation of a new removal method of osseointegrated implants under a minimally invasive technique. MATERIALS AND METHODS The study was conducted on a group of 66 osseointegrated internal and external connection implants that were considered unsuccesful. The surgical removal was indicated after radiologically confirming peri-implant bone loss, and poor positioning which made the prosthetic rehabilitation impossible, or would damage the prosthetic implant retainer. The surgical procedure was developed in an operating room under local anesthesia. As first surgical removal option, the procedure consisted of the use of a removal instrument that works against torque and which does not need a full thickness flap to reach the surgical site; and as a second surgical removal option a trephine bur was used. For this option, a full thickness flap was performed in order to expose the surgical site. RESULTS A total of 66 osseointegrated implants were removed under the first mode, with no full thickness flaps or Trephine burs required. CONCLUSIONS Within the limitations of this preliminary study, we propose that the use of this new, against torque, removal instrument of osseointegrated implants in failure is seen as a very good alternative to invasive surgical removal techniques, reducing surgical risks.

Influência do modelo e marca de ponta diamantada na resistência flexural / Influence of model and brand of diamond bur in flexural strength

Introdução: As pontas diamantadas são instrumentos rotatórios abrasivos utilizados principalmente na odontologia restauradora para realização de preparos dentários e acabamentos de restaurações. Estes instrumentos são formados por uma haste metálica, eixo intermediário e ponta ativa. Objetivo: Mensurar a resistência flexural da haste e intermediário de pontas diamantadas de seis marcas comerciais diferentes disponíveis no mercado brasileiro. Materiais e Métodos: utilizou- se 60 pontas diamantadas, sendo 30 pontas 2200 (n=5) e 30 pontas 2130 (n=5), das marcas KG Sorensen, Option, Microdont, FAVA, Zeep, Vortex. Os instrumentos rotatórios foram posicionados em dispositivo que permitiu que suas extremidades fossem apoiadas sobre bases metálicas; submetidas à Máquina Universal de Ensaio (EMIC), para mensurara força máxima aplicada em um deslocamento de 3 mm. Para o padrão de falha, verificou-se se as pontas sofreram dobras ou fraturas totais. Resultado: os instrumentos rotatórios da marca KG Sorensen destacaram-se em todos os testes, apresentando elevados valores de resistência flexural tanto para haste quanto para intermediário; não apresentando nenhuma falha do tipo fratura total. A marca Option apresentou o maior índice de instrumentos com falha do tipo fratura total.Conclusão: não existe padronização da resistência flexural entre as marcas comercias, sendo que a utilização de instrumentos com baixa resistência pode ocasionar maiores taxas de falha, danificando o equipamento ou causar injurias aos tecidos do paciente.

Introduction: The diamond burs are rotary abrasives instruments primarily used in restorative dentistry to perform dental preparations and finishing restorations. These instruments consist of shank, intermediate shaft and head. Objective: To measure the flexural strength of the shank and intermediate shaft diamond burs of six different commercial brands available in the Brazilian market. Methods: 60 diamond burs, 30 burs 2200(n = 5) and 30 burs 2135 (n = 5), of brands KG Sorensen, Option, Microdont, FAVA, Zeep, Vortex were used. The rotary instruments were placed in the device that allowed its ends were supportedon metal bases; submitted to Universal Testing Machine (EMIC), to measure the maximum force applied a displacement of 3mm. For the standard fault, it was found that the burs suffered bends or total fractures. Results: It was observed that the rotary instruments KG Sorensen, stood out all tests, showing high values of flexural strength for shank and intermediate shaft; showing no fault of type fracture total. Option had the highestrate of failure type instruments with total fracture. Conclusion: There is no standardization between the flexural trademarks, and the use of instruments with low resistance may cause higher rates of failure, damaging the equipment or cause injuries to the tissues of the patient.

Ultrasonic cavity preparation using CVD coated diamond bur: A case report.

Before any restorative procedure can be undertaken a proper cavity preparation is required. This clinical step is the mechanical alteration of the tooth to receive a restorative material with which a satisfactory form, function and the esthetics of the tooth will be established. In recent years improvements in materials and techniques have been devised and new technologies are now available for this purpose. The aim of the present study is to report two clinical cases in which a CVD coated diamond bur coupled to an ultrasonic handpiece is used in dental preparation. This technique provides an accurate and conservative tooth preparation with ideal access and visibility and because of enhanced efficiency can also play a role in eliminating some of the patient discomfort of the dental treatment.

Técnica pessoal para obtenção de enxertos ósseos cranianos / Personal technique to harvest skull bone grafts

O objetivo deste artigo foi relatar o desenvolvimento de técnica pessoal para obtenção de enxerto ósseo da calota craniana e divulgá-la entre os neurocirurgiões brasileiros. Essa técnica foi empregada em 101 pacientes que apresentavam necessidade de reconstrução óssea da maxila e/ou da mandíbula. A técnica apresenta o diferencial de determinação da profundidade do crânio por meio de trepanações parciais realizadas na região anterior e posterior do osso parietal para, posteriormente, realizar a remoção do enxerto. Como conclusão, a técnica mostra-se segura e eficiente e pode ser aplicada a qualquer paciente que necessita de cirurgia reconstrutiva.

The objective of this paper was to report the development of personal technique to harvest skull bone graft and spread it to Brazilian neurosurgeons. This technique was used in 101 patients who needed reconstructive surgery prior dental implants. The technique presents like differentiate the clinical determination of thickness of cranium prior the osteotomy. This technique, like conclusion, is safe and efficient and can be applied in any reconstructive surgery.

Levantamento do seio atraumático utilizando broca trefina: apresentação de técnica / Atraumatic sinus lift using a trefine bur: technique introduction

Os autores relatam em um caso clínico, uma nova técnica cirúrgica de levantamento de seio atraumático, realizado em uma paciente do sexo feminino que apresentava ausência do elemento dentário 25 havendo 9 mm de disponibilidade óssea da crista alveolar até o assoalho do seio maxilar, utilizando broca tipo trefina associada aos osteótomos côncavos o que permitiu a instalação de um implante de 14 mm

The present paper describes a clinical case of a new atraumatic sinus lift technique. Using a trefine bur and concave osteotomes, one patient with 9 mm of residual alveolar bone height underwent an atraumatic sinus augmentation surgery and an endosseous implant (14 mm) was inserted simultaneously

Influência da técnica de preparo cavitário na microinfiltração marginal em restaurações de dentes decíduos / Evaluation of the cavity prepare shape on the marginal microleakage in deciduous teeth

O objetivo desse trabalho foi avaliar a influência da forma do preparo cavitário na microinfiltração marginal em restaurações em dentes decíduos. Foram preparadas canaletas medindo 2,0 mm nas faces vestibular e lingual de molares decíduos que foram divididas aleatoriamente em 3 grupos de acordo com a técnica de preparo cavitário: Grupo I - alta rotação associado à ponta diamantada; Grupo II ­ sistema de abrasão a ar; e Grupo III ­ laser Er:YAG. Após o condicionamento com ácido fosfórico por 15 segundos, foi utilizado o adesivo Single Bond (3M) e a resina Filtek Flow (3M) para restaurar as canaletas. Os espécimes foram então mantidos em estufa a 37o C por 6 dias, termociclados (500 ciclos com temperatura variando entre 5 e 55o C), isolados e colocados em solução de fucsina básica à 0,5% durante 24 horas. Após serem seccionados a microinfiltração foi avaliada em microscópio ótico ligado a uma câmera de vídeo com 10 X de aumento. Para se avaliar a forma das cavidades medimos os ângulos oclusal, cervical e de abertura das cavidades, bem como a largura e a profundidade das mesmas por meio de fotomicrografias obtidas em MEV. Os resultados demonstraram semelhança estatística entre as larguras dos preparos cavitários, porém os ângulos oclusal, cervical e de abertura das cavidades apresentaram-se diferentes estatisticamente e que não houve microinfiltração marginal em nenhum dos grupos avaliados e que a diferença na forma de preparo das cavidades não influenciou na microinfiltração marginal dos espécimes estudados

The aim of this research was the evaluation of the cavity prepare technique on the marginal microleakage in deciduous teeth. Cavities measuring 2.0 mm were prepared on the buccal and lingual faces and were divided into three groups according to the cavity prepare technique: Group I ­ High speed associated to diamond bur; Group II ­ air abrasion system; and Group III ­Er:YAG laser. After the phosphoric acid etching for 15 seconds, the Single Bond (3M) adhesive and the Filtek Flow (3M) were used for the restoration of the cavities. Then the sample was stored at 37o C for 6 days, termocycled (500 cycles with temperature varying from 5 to 55o C), isolated and immersed in a 0.5% fucsin solution for 24 hours. After being sectioned the microleakage was examined with a magnification optical microscope connected to a color video camera. In order to evaluate the shape of the cavities the occlusal, cervical and cavity opening angles were measured, as well as the width and depth of then, using SEM photographs. The results showed that there was no marginal microleakage in any of the evaluated groups and that the difference in the cavity shape did not influence the marginal infiltration on the studied sample