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1.
J Colloid Interface Sci ; 677(Pt B): 303-311, 2025 Jan.
Article in English | MEDLINE | ID: mdl-39146818

ABSTRACT

HYPOTHESIS: Perfluorocarbon is commonly used as a coolant, chemical reaction carrier solvent, medical anti-hypoxic agents and blood substitutes. The realization of non-contact complex manipulation of perfluorocarbon liquids is urgently needed in human life and industrial production. However, most liquid-repellent interfaces are ineffective for the transport of ultra-low surface tension perfluorocarbon liquids, and struggle to maintain good durability due to unstable air or oil cushions in the surface. Therefore, preparing surfaces for stable non-contact complex manipulation of ultra-low surface tension droplets remains a challenge. EXPERIMENTS: In this paper, a novel solution, a photothermal responsive droplet manipulation surface based on polydimethylsiloxane brushes, has been reported. On this surface, droplets with different surface tensions (as low as 10 mN/m) can be efficiently manipulated through induced near-infrared light. Notably, this surface maintains its effectiveness after exposure to extreme anthropogenic conditions. FINDINGS: The interface effect between perfluorocarbon droplets and polydimethylsiloxane brushes by near-infrared light-induced was investigated in detail. In addition, ultra-low surface tension droplets demonstrate the ability to transport solid particles. The conductive droplets exhibit sophisticated manipulation realizing the controlled switching of smart circuits. This research opens up new possibilities for advancing the capabilities and adaptability of ultralow surface tension droplets in a range of applications.

2.
Health Sci Rep ; 7(10): e70124, 2024 Oct.
Article in English | MEDLINE | ID: mdl-39391676

ABSTRACT

Background and Aim: Premenstrual tension syndrome can vary in type and severity among females, potentially affecting their academic performance. This study aims to examine the prevalence and severity of premenstrual tension syndrome symptoms in female university students and their impact on academics. Methods: A cross-sectional descriptive study was conducted among female university students from Ras Al Khaimah, UAE over 6 months, from January 1, 2022 to June 30, 2022. The updated premenstrual tension syndrome self-rating scale questionnaire was used to assess the prevalence and severity of symptoms. A total of 251 respondents were included in the study. The chi-square test was used to determine the association between PMT and academic performance. Results: The results showed that 78.9% of the participants experienced premenstrual tension syndrome, with 16.3% of them reporting premenstrual dysphoric disorder. The majority of participants reported mild to moderate symptoms, with anxiety and depressed mood being the most common psychological symptoms. In terms of behavioral symptoms, physical symptoms and feeling overwhelmed were the most prevalent. Furthermore, 90% of participants reported a negative impact on their academic performance, with paying attention in class being the most affected. The study also found that PMT disorder had a significant impact on physical activities and extracurricular activities. Conclusions: Our study showed a high prevalence of premenstrual disorders among female students and their influence on physical activity and extracurricular participation. The study highlights the importance of implementing therapies for PMT syndrome and providing support to improve academic performance and overall quality of life.

3.
Sci Rep ; 14(1): 23462, 2024 Oct 08.
Article in English | MEDLINE | ID: mdl-39379447

ABSTRACT

Current research introduces understanding of dental-cracks mechanistic with fundamental fracture behavior in natural-teeth and orthodontics including mode I crack under both tension and compression, mode II crack under both clockwise-shear and anticlockwise-shear and mixed-mode cracks under both compression-shear and tension-shear. It depends on experimental models of transparent-Plexiglas including pre-cracks of different orientations angle (b) based on fundamental theoretical fracture analysis with comparison. Problem-concept, cracking aspects of fracture-initiation, propagation-direction, fracture-increment length, critical external-load and fracture path are predicted experimentally and theoretically using directional fracture approach and directional strain-energy density theory. Tests are carried out for (36) samples for compression and tension in LEFM. Friction-resistance between crack-surfaces is considered with derivation of equations and charts. Negative stress-intensity factor (-KI) is developed for solving complicated problems of cracks under occlusal compression loads. The occlusal loads are compression and shear producing lateral tensile mixed mode cracks. The critical propagation angle (qc), critical propagation load (sc) and critical propagation envelope of stress intensity factors (KI-KII) are developed with respect to crack orientation angle (b) with comparisons. They are necessary to predict the fracture propagation early before teeth-failure. It helps for prevention and control of dental-cracks, correct-restoration, prosthodontics, orthodontics, and development of new dental-materials and technologies.

4.
J Orthop Case Rep ; 14(10): 230-235, 2024 Oct.
Article in English | MEDLINE | ID: mdl-39381312

ABSTRACT

Introduction: The inferior pole of patella constitutes around 9.3-22% of all the patella fractures presenting to the hospital. The management of inferior pole fractures has been debated since a long from early day of partial or full patellectomy to the current dictum of open reduction and fixation because of the importance of extensor mechanism of the knee. There are various modalities of management of the inferior pole of patella fracture. The use of braided polyester wire has shown to have a higher load to failure and less implant fatigue, leading to the use of trams osseous repair technique with fiber wire as an alternative to tension ban wiring. Materials and Methods: Twenty patients were divided into two groups of 10 each: Group A comprised of patients operated with tension band wiring (TBW) and Group B with patient operated with transosseous repair. Both groups underwent the same physiotherapy postoperatively and the results were compared. Results: Radiological outcomes and the WOMAC score of the patients are comparable in both groups. The return to full range of motion is also similar in both groups with similar physiotherapy given in both groups. Conclusion: This shows that with careful patient selection transosseous repair can be used as an alternative to TBW.

5.
J Hand Surg Glob Online ; 6(5): 691-693, 2024 Sep.
Article in English | MEDLINE | ID: mdl-39381391

ABSTRACT

Peripheral nerve injuries that are not acutely repaired may lead to a nerve gap because of the surrounding zone of injury and elastic recoil of nerve tissue after laceration. This can result in tension across the repair site during primary neurorrhaphy. Decades of basic science literature using in vivo models consistently demonstrate a relationship between increasing strain at a neurorrhaphy site and compromised microvascular blood flow. Clinical and laboratory data suggest tension-free repairs are associated with optimal outcomes; in the setting of a short segmental nerve gap, data suggest primary repair may continue to yield good functional results. In the case of high strain, nerve grafting or other methods should be considered given poor results of primary repairs performed under high tension because of local ischemia and fibrosis on a cellular level.

6.
Clin Exp Ophthalmol ; 2024 Oct 09.
Article in English | MEDLINE | ID: mdl-39385332

ABSTRACT

BACKGROUND: To evaluate and compare the long-term efficacy of medical treatments for normal tension glaucoma (NTG) in controlling intraocular pressure (IOP), and establish a hierarchical ranking based on their effectiveness. 'Long-term' is defined as a treatment duration of over 12 weeks in randomised controlled trials (RCTs). METHODS: This systematic review and model-based network meta-analysis (MBNMA) collected data of 795 patients with 997 eyes from RCTs. Patients with NTG were selected based on strict inclusion/exclusion criteria, with randomsation procedures and masking as reported in the individual trials. Eight different medications were compared, including prostaglandin analogues, beta-blockers, brimonidine, unoprostone isopropyl, brovincamine, and palmitoylethanolamide (PEA). Notably, PEA is an oral medication, while other drugs are topical agents. RESULTS: Primary outcome is the long-term efficacy of IOP control across medications with different follow-up durations. Among the eight medications, PEA demonstrates the highest efficacy (Surface under the cumulative ranking, SUCRA = 7.46%), followed by two prostaglandin analogues: travoprost (SUCRA = 6.86%) and latanoprost (SUCRA = 6.76%), then two beta-blockers: nipradilol (SUCRA = 4.90%) and timolol (SUCRA = 4.89%). Both brimonidine and unoprostone isopropyl have SUCRA scores below 4.0%, indicating modest but limited efficacy. Brovincamine has the lowest SUCRA score (1.32%), reflecting minimal effectiveness. CONCLUSIONS: This study revealed PEA as a promising agent for long-term IOP control in NTG patients, suggesting potential use as primary or adjunctive therapy. The outcomes call for PEA's consideration in clinical practice and highlight the need for further research into its long-term efficacy and safety for NTG.

7.
Sex Med Rev ; 2024 Oct 10.
Article in English | MEDLINE | ID: mdl-39385631

ABSTRACT

INTRODUCTION: Primary headaches, particularly migraine and tension-type headaches, as chronic and painful conditions, have a significant impact on individuals' health and overall quality of life, including aspects such as sexual health parameters. OBJECTIVES: The aim of this study was to review observational studies and to summarize the presence of sexual dysfunction in patients with primary headache and to discern whether it is more common in patients with primary headache than in healthy control subjects. METHODS: Observational studies published between January 1, 2000, and May 21, 2023 were searched in Medline (PubMed), Web of Science, Scopus, and EBSCO. This review included 23 observational studies that evaluated sexual dysfunction with or without comparison with healthy control subjects. RESULTS: As a result, the mean Female Sexual Function Index score, a score of <26.55 indicating sexual dysfunction, ranged from 19.25 ± 8.18 to 27.5 ± 7.5 in patients with primary headache. The prevalence of sexual dysfunction and erectile dysfunction was found to be between 10.7% and 93.75% in female patients and between 54.83% and 80% in male patients. Both migraineurs and tension-type headache sufferers had statistically significantly lower sexual function scores in females and lower erectile function scores in males compared with healthy control subjects. CONCLUSION: Sexual function is significantly affected in patients with primary headache, which is a chronic painful disorder. Therefore, it is very important to ask these patients about their sexual health and, if necessary, to seek support from a sexual health professional. Additionally, future studies may prioritize tension-type headaches and male patients due to the limited amount of research available on these subjects.

8.
Sci Rep ; 14(1): 23161, 2024 Oct 05.
Article in English | MEDLINE | ID: mdl-39369023

ABSTRACT

To explore the influence of surfactant concentration on the pore structure and permeability of coal samples during the chemical enhancement of coalbed methane production, different kinds and different concentrations of surfactants were added to the chemical solution, and the coal samples were soaked. Methods such as low-field nuclear magnetic resonance testing (NMR), fractal theory, permeability testing, surface tension testing, and contact angle testing were employed to analyze the variation patterns of coal sample pore structure, fractal characteristics, and permeability, and to explore the correlation between surface tension, contact angle, and the degree of pore structure development. The results show that the increase in total porosity of coal samples, the increase in the seepage pore porosity, the decrease in Dt, and the growth rate of permeability increase with the increase in surfactant concentration, and are negatively correlated with the surface tension of the solution and the contact angle of the coal-solution interface, while the decrease in Ds is not significantly correlated with surfactant concentration, surface tension, or contact angle. In terms of the erosion effect of a chemical solution on coal samples, the influence of contact angle is greater than that of surface tension, while surface tension has the greatest impact on the development of adsorption pores. By adding different surfactants, the surface tension of the chemical solution and the contact angle of the coal-solution interface can be controlled, further promoting the erosion of coal samples, which is of positive significance for the chemical enhancement of coalbed methane production.

9.
Sci Rep ; 14(1): 23220, 2024 Oct 05.
Article in English | MEDLINE | ID: mdl-39369130

ABSTRACT

Molecular dynamics (MD) simulation of the green ionic liquid [C4mim][PF6] mixed with polar benzonitrile (BNZ) solvent provides detailed insights into their structural and dynamic properties, essential for electrochemistry and materials science applications. The simulations we carried out at varying mole fractions (XBZN) reveal the mixtures' physical, structural, and dynamic properties, with radial, spatial, and combined distribution functions, highlighting the effective interaction through H-bonding involved. The simulation indicates that BZN stacks on the cation butyl tail, providing a significant explanation for the unique experimental observations (following). Adding BZN causes the mixture's liquid dynamics to increase linearly at low XBZN and exponentially at high XBZN, with a notable singular transition at 0.5XBZN. Comprehensive efforts were made to verify and support experimental sum frequency generation (SFG) spectroscopy by simulating the surface structure of the mixtures. Consequently, the simulated BZN stacking structure explains (1) the absence of the C≡N vibrational mode in the SFG spectrum for XBZN < 0.8, and (2) the gradual diminishing of the CH3 SFG signal, which disappears as XBZN approaches 0.5. Finally, this research removes a persistent ambiguity, proving that only the molecular moieties on the surface generate the SFG vibrational signal, while those in the subsurface do not.

10.
Indian J Otolaryngol Head Neck Surg ; 76(5): 3777-3792, 2024 Oct.
Article in English | MEDLINE | ID: mdl-39376292

ABSTRACT

Background: Laryngeal manual therapy (LMT) has been known as one of the main techniques for decreasing musculoskeletal tension in the (para) laryngeal muscles in dysphonic patients, especially patients with muscle tension dysphonia (MTD). Objective: A narrative review on the available LMT methods in MTD. Study Design: (Method).A review on the literature regarding to the LMT methods in MTD was conducted using electronic databases up to December 2022. To identify all eligible literature, hand searching was also utilized. Moreover, articles and books in which LMT methods were introduced or used for the first time, or had innovation and were complementary for previous LMT methods were included. Relevant sources were identified by two reviewers based on screened titles/abstracts and full texts. Results: The authors found five main common and some miscellaneous LMT methods that may not be used as conventional methods in clinical and research settings. There are some similarities and differences between the available LMT methods based on the manual techniques, target anatomical structures, and tension criteria. Although there is not enough information about details of some LMT methods, we additionally tried to provide some details about LMT treatment protocol. Conclusion: The LMT is still at the beginning and it is necessary to revise the previous methods or develop new ones according to the similarities and differences have been found in this review. This paper will be useful for learning and teaching the LMT methods in MTD for clinicians and students who are interested in. Supplementary Information: The online version contains supplementary material available at 10.1007/s12070-024-04896-1.

11.
Indian J Otolaryngol Head Neck Surg ; 76(5): 3940-3943, 2024 Oct.
Article in English | MEDLINE | ID: mdl-39376346

ABSTRACT

To explore the risk of obstructive sleep apnea (OSA) in patients with primary muscle tension dysphonia (MTD). The medical records of patients diagnosed with primary MTD between November 2021 and March 2023, were reviewed. The risk of having OSA was assessed by looking at the scores of two validated questionnaires, namely the STOP-BANG questionnaire and the Berlin questionnaire. A total of 40 patients with primary MTD were enrolled in this study, including 16 females (40%) and 24 males (60%). The mean total STOP-BANG score was 3.57 ± 1.67. Seventeen of the study group had intermediate risk of OSA, and 14 had high risk, accounting for 77.5% of patients with primary MTD. When using the Berlin questionnaire, the mean total score was 1.65 ± 0.95, and 67.5% of the study group had a score > 2 and were at high risk of having OSA. The results of this study indicate that patients with primary MTD are at moderate to severe risk of having OSA.

12.
Front Pediatr ; 12: 1484562, 2024.
Article in English | MEDLINE | ID: mdl-39376674

ABSTRACT

[This corrects the article DOI: 10.3389/fped.2023.1269628.].

13.
Int Urogynecol J ; 2024 Oct 01.
Article in English | MEDLINE | ID: mdl-39352427

ABSTRACT

INTRODUCTION AND HYPOTHESIS: Challenges in intracorporeal knot tying can be addressed with extracorporeal slip knots, simplifying the process of tying and managing tissue tension using a knot pusher. However, existing extracorporeal knot techniques are difficult owing to their complexity, the finesse required with thin yarns, and extensive training needs. We developed a new laparoscopic extracorporeal slip knot technique that can be used with a conventional needle driver or standard clamps, offering the advantages of being cost-effective and easy to learn. METHODS: The technique involves passing the active strand over another loop and securing it with the nondominant hand. A Kelly clamp is then wound around both loops three times in a tornado-like motion, passing the instrument over the active loop and under the passive one, and grasping the active strand. The passive strand is pulled to approximate the knot to the tissue and is tightened by the tension of the passive strand. RESULTS: This method has proven effective in various laparoscopic procedures such as sacrocolpopexy, colposuspension, pectopexy, myomectomy, and hysterectomy, facilitating surgeries without complications. CONCLUSIONS: The tornado knot technique is a feasible and safely locked sliding extracorporeal knot that can be easily learned, especially by surgeons who are accustomed to open surgery.

14.
Int Urogynecol J ; 2024 Oct 01.
Article in English | MEDLINE | ID: mdl-39352429

ABSTRACT

INTRODUCTION AND HYPOTHESIS: The objective was to determine if mid-urethral sling (MUS) tensioning with a Mayo Scissor as a sub-urethral spacer compared with a Babcock clamp holding a loop of tape under the urethra results in differences in patient-reported outcomes and rates of repeat surgery over a 5-year follow-up. METHODS: Follow-up 5 years after a randomized clinical trial, utilizing primary data collection linked to administrative health data, was carried out to create a longitudinal cohort. The primary outcome was participant-reported bothersome SUI symptoms, as defined by the Urogenital Distress Inventory (UDI-6) questionnaire. Secondary outcomes included participant-reported bothersome overactive bladder (OAB) scores, median scores of three validated urinary symptom questionnaires, and rates of subsequent surgery determined through patient report and administrative data. RESULTS: Two hundred and sixty (81.8%) of the original study participants provided participant-reported data at 5 years. Administrative data linkage was completed for all of the original participants (n = 318). Demographic characteristics remained similar in the two groups at the 5-year follow-up mark. No differences existed in the primary outcome of reported bothersome SUI symptoms (30.8% Scissors vs 26.8% Babcock, p = 0.559), proportion of participants with bothersome OAB, the median scores of three validated bladder questionnaires, or in rates and cumulative incidence of recurrent MUS surgery or surgical revision of mesh-related complications. CONCLUSION: Both the Scissor and Babcock tensioning techniques provided comparable outcomes at 5 years post-MUS surgery. The information from this study allows surgeons to better decide which technique to adopt in their practice, providing confidence in longer-term cure and safety.

15.
Int J Emerg Med ; 17(1): 134, 2024 Oct 03.
Article in English | MEDLINE | ID: mdl-39363258

ABSTRACT

BACKGROUND: Congenital diaphragmatic hernia(CDH) is a rare congenital anomaly characterized by herniation of abdominal contents into thoracic cavity through a defect in diaphragm. While commonly diagnosed prenatally or in neonatal period, late-presenting CDH can occur and may mimic other thoracic emergencies such as tension pneumothorax, complicating diagnosis and management. CASE PRESENTATION: A two-year old male black child from Ethiopia presented to the emergency department with sudden onset of acute respiratory distress. Initial clinical assessment and chest radiography suggested a diagnosis of tension pneumothorax due to the presence of significant mediastinal shift and apparent pleural air. Despite insertion of chest tube, the child's condition did not improve, raising suspicion of alternative diagnosis. Careful observation of initial chest x-ray and subsequent chest ultrasound revealed a left sided congenital diaphragmatic hernia with herniation of stomach and intestine into thoracic cavity compressing the left lung and causing mediastinal shift. After the diagnosis of CDH was confirmed, the child was stabilized and emergent surgical repair performed. Postoperative recovery was uneventful, and the child was discharged with no significant long-term complications. CONCLUSION: This case underscores the importance of considering CDH in the differential diagnosis of acute respiratory distress in a child. It highlights the diagnostic challenges and potential risks of emergency interventions based on initial misdiagnosis. Even if x -ray looks like typical of tension pneumothorax, it showed giant cystic air filled structure pushing the mediastinal structure to contralateral side with loss of left diaphragmatic outline which raised suspicion of congenital cystic lung mass or congenital diaphragmatic hernia. Advanced imaging and high index of suspicion are crucial for accurate diagnosis and timely management, ultimately improving patient outcomes. Consideration of alternative diagnosis when our initial intervention with insertion of chest tube fail to provide symptom improvement in suspected pneumothorax should raise suspicion of congenital diaphragmatic hernia like in our case.

16.
J Cell Sci ; 2024 Oct 04.
Article in English | MEDLINE | ID: mdl-39369303

ABSTRACT

Nonmuscle myosin II generates cytoskeletal forces that drive cell division, embryogenesis, muscle contraction, and many other cellular functions. However, at present there is no method that can directly measure the forces generated by myosins in living cells. Here we describe a Förster resonance energy transfer (FRET)-based tension sensor that can detect myosin associated force along the filamentous actin network. Fluorescence lifetime imaging microscopy (FLIM)-FRET measurements indicate that the forces generated by NMIIB exhibit significant spatial and temporal heterogeneity as a function of donor lifetime and fluorophore energy exchange. These measurements provide a proxy for inferred forces that vary widely along the actin cytoskeleton. This initial report highlights the potential utility of myosin-based tension sensors in elucidating the roles of cytoskeletal contractility in a wide variety of contexts.

17.
Article in English | MEDLINE | ID: mdl-39369428

ABSTRACT

PURPOSE: Unicondylar arthroplasty was performed using robotic medial unicompartmental knee arthroplasty (R-mUKA) and gap-balancing instrumentation. Our hypothesis was that robotic unicondylar knee arthroplasty accurately restores component positioning and lower limb alignment when compared to preoperative planning with actual implantation throughout the range of knee motion due to proper knee balancing. METHODS: Data were collected prospectively and were analysed for patients undergoing RM-UKA. A cemented UKA was implanted using the MAKO® robotic system. Lower limb alignment at 0°, 30°, 45°, 60° and 90° of flexion was recorded of the native knee, with the trial components in place and finally after component implantation. A spacer according to the femorotibial gap was introduced and the alignment was measured. The position of the final component was planned based on three-dimensional computed tomography images before making the bone cuts. The positioning of the femoral and tibial components was analysed in all three planes. RESULTS: A total of 52 patients were included (mean age 66.3 ± 6.7 years; 34 males, 18 females). The difference in femoral component position after planning and final implantation was 0.04° ± 0.58° more valgus in the coronal plane (p = 0.326) and 0.6° ± 1.4° more flexion relative to the sagittal plane (p = 0.034). The tibial component was placed in the coronal plane in 0.3° ± 0.8° of more varus (p = 0.113) and in the sagittal plane in 0.6° ± 1.2° of more posterior tibial slope (p = 0.001). Lower limb alignment of the native knee in extension was 5.8° ± 2.6° of varus and changed to 3° ± 2.1° varus after UKA (p ≤ 0.01). CONCLUSION: R-mUKA helps to achieve the target of alignment and component position without any significant differences to the planning. Ligament balancing causes non-significant changes in component position. It allows optimal component position even for off-the-shelf implants respecting the patient's specific anatomy. LEVEL OF EVIDENCE: II.

18.
BMC Ophthalmol ; 24(1): 427, 2024 Oct 01.
Article in English | MEDLINE | ID: mdl-39354448

ABSTRACT

BACKGROUND: Bilateral malignant glaucoma induced by a capsular tension ring associated with ring-shaped cysts of the ciliary body post-cataract surgery is rare. Herein, we present a case to highlight the possibility of capsular tension ring-induced malignant glaucoma. CASE PRESENTATION: A 59-year-old woman underwent phacoemulsification combined with capsular tension ring implantation for cataracts and zonular fibre laxity in both eyes. Upon admission, annular ciliary masses were detected in both eyes using ultrasound biomicroscopy. Two months post-surgery, the patient experienced vision deterioration, high intraocular pressure, and an axial shallowing anterior chamber in the right eye, and responded poorly to traditional anti-glaucoma medication. Ten days later, similar symptoms appeared in the left eye. Ultrasound biomicroscopy detected contact between the ciliary body and the capsular tension ring. Subsequently, malignant glaucoma was diagnosed. Anterior and posterior capsulotomies performed peripheral to intraocular lens optics using neodymium: YAG laser restored communication and alleviated the symptoms. A one-year follow-up revealed stable intraocular pressure and anterior chamber in both eyes. CONCLUSIONS: This is the first case report of bilateral malignant glaucoma after cataract surgery induced by capsular tension ring, which is associated with bilateral ring-shaped cysts of the ciliary body. Blockage between the ciliary body and capsular tension ring was confirmed using ultrasound biomicroscopy.


Subject(s)
Ciliary Body , Glaucoma , Intraocular Pressure , Phacoemulsification , Humans , Female , Middle Aged , Intraocular Pressure/physiology , Glaucoma/etiology , Glaucoma/surgery , Phacoemulsification/adverse effects , Ciliary Body/diagnostic imaging , Microscopy, Acoustic , Postoperative Complications , Lens Implantation, Intraocular/adverse effects , Cataract/etiology , Prostheses and Implants/adverse effects , Prosthesis Implantation/adverse effects
19.
Proc Natl Acad Sci U S A ; 121(41): e2316450121, 2024 Oct 08.
Article in English | MEDLINE | ID: mdl-39356672

ABSTRACT

Deciphering the dynamic mechanism of ferroptosis can provide insights into pathogenesis, which is valuable for disease diagnosis and treatment. However, due to the lack of suitable time-resolved mechanosensitive tools, researchers have been unable to determine the membrane tension and morphology of the plasma membrane and the nuclear envelope during ferroptosis. With this research, we propose a rational strategy to develop robust mechanosensitive fluorescence lifetime probes which can facilitate simultaneous fluorescence lifetime imaging of the plasma membrane and nuclear envelope. Fluorescence lifetime imaging microscopy using the unique mechanosensitive probes reveal a dynamic mechanism for ferroptosis: The membrane tension of both the plasma membrane and the nuclear envelope decreases during ferroptosis, and the nuclear envelope exhibits budding during the advanced stage of ferroptosis. Significantly, the membrane tension of the plasma membrane is always larger than that of the nuclear envelope, and the membrane tension of the nuclear envelope is slightly larger than that of the nuclear membrane bubble. Meanwhile, the membrane lesions are repaired in the low-tension regions through exocytosis.


Subject(s)
Cell Membrane , Ferroptosis , Fluorescent Dyes , Microscopy, Fluorescence , Nuclear Envelope , Ferroptosis/physiology , Humans , Fluorescent Dyes/chemistry , Cell Membrane/metabolism , Nuclear Envelope/metabolism , Microscopy, Fluorescence/methods , Exocytosis/physiology , HeLa Cells
20.
J Biomech ; 176: 112328, 2024 Sep 21.
Article in English | MEDLINE | ID: mdl-39357344

ABSTRACT

The biomechanical rupture risk assessment (BRRA) of abdominal aortic aneurysms (AAA) has higher sensitivity than maximal diameter criterion (DSEX) but its estimation is time-consuming and relies on an uncertain estimation of wall thickness. The aim of this study is to test tension-based criterion in the BRRA of AAA which removes the necessity of wall thickness measurement and should be faster. For that, we retrospectively analyzed 99 patients with intact AAA (25 females). Nineteen of them experienced a rupture later. BRRA was performed with wall tension PRRIT as a primary criterion. The ability of criterion to separate intact and ruptured AAAs at 1,3,6,9 and 12 months was estimated. Next, the receiver operating characteristics and the percentage of true negative cases for a different time to an outcome were estimated. Finally, the computational time was recorded. The results were compared to stress-based criterion PRRI and DSEX which served as a reference. All three criterions were able to discriminate between intact and ruptured AAAs up to 9 months (p < 0.05) while none of them could do for a 12 month prediction. PRRIT exhibited a significantly higher percentage of true negatives for 12 and 9 month predictions (45 % and 20 % respectively) and similar to other criteria for other prediction times. The mean computational time for estimating PRRIT was 19 h per patient compared to 67 h for PRRI. The tension- based BRRA of AAA leads to better outcomes for a 9 and 12 month prediction while the computational time drops by more than 70 % compared to PRRI.

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