A Novel Experimental Apparatus for Characterizing Flow Regime in Mechanically Stirred Tanks through Force Sensors.

Pressure fluctuations in a mixing tank can provide valuable information about the existing flow regime within the tank, which in turn influences the degree of mixing that can be achieved. In the present work, we propose a prototype for identifying the flow regime in mechanically stirred tanks equipped with four vertical baffles through the characterization of pressure fluctuations. Our innovative proposal is based on force sensors strategically placed in the baffles of the mixing tank. The signals coming from the sensors are transmitted to an electronic module based on an Arduino UNO development board. In the electronic module, the pressure signals are conditioned, amplified and sent via Bluetooth to a computer. In the computer, the signals can be plotted or stored in an Excel file. In addition, the proposed system includes a moving average filtering and a hierarchical bottom-up clustering analysis that can determine the real-time flow regime (i.e., the Reynolds number, Re) in which the tank was operated during the mixing process. Finally, to demonstrate the versatility of the proposed prototype, experiments were conducted to identify the Reynolds number for different flow regimes (static, laminar, transition and turbulent), i.e., 0≤Re≤ 42,955. Obtained results were in agreement with the prevailing consensus on the onset and developed from different flow regimes in mechanically stirred tanks.

Prediction of the Limiting Flux and Its Correlation with the Reynolds Number during the Microfiltration of Skim Milk Using an Improved Model.

Limiting flux (JL) determination is a critical issue for membrane processing. This work presents a modified exponential model for JL calculation, based on a previously published version. Our research focused on skim milk microfiltrations. The processing variables studied were the crossflow velocity (CFV), membrane hydraulic diameter (dh), temperature, and concentration factor, totaling 62 experimental runs. Results showed that, by adding a new parameter called minimum transmembrane pressure, the modified model not only improved the fit of the experimental data compared to the former version (R2 > 97.00%), but also revealed the existence of a minimum transmembrane pressure required to obtain flux (J). This result is observed as a small shift to the right on J versus transmembrane pressure curves, and this shift increases with the flow velocity. This fact was reported in other investigations, but so far has gone uninvestigated. The JL predicted values were correlated with the Reynolds number (Re) for each dh tested. Results showed that for a same Re; JL increased as dh decreased; in a wide range of Re within the turbulent regime. Finally, from dimensionless correlations; a unique expression JL = f (Re, dh) was obtained; predicting satisfactorily JL (R2 = 84.11%) for the whole set of experiments.

On the motion of magnetotactic bacteria: theoretical predictions and experimental observations.

The movement of magnetotactic bacteria is done in a viscous media in the low Reynolds number regime. In the present research, the simple model for magnetotactic bacteria motion, proposed by Nogueira and Lins de Barros (Eur Biophys J 24:13-21, 1995), was used to numerically simulate their trajectory. The model was done considering a spherical bacterium with a single flagellum and a magnetic moment positioned in the sphere center and parallel to the flagella. The numerical solution shows that the trajectory is a cylindrical helix and that the body Euler angles have linear dependencies on time. Using that information, analytical expressions were obtained for the first time for the center-of-mass coordinates, showing that the trajectories are helixes oriented to the magnetic field direction. They also show that the magnetic moment does not align to the magnetic field, but it precesses around it, being fully oriented only for very high magnetic fields. The analytical solution obtained permits to relate for the first time the flagellar force to the axial velocity and helical radius. Trajectories of uncultivated magnetotactic bacteria were registered in video and the coordinates were obtained for several bacteria in different magnetic fields. The trajectories showed to be a complex mixture of two oscillating functions: one with frequency lower than 5 Hz and the other one with frequency higher than 10 Hz. The simple model of Nogueira and Lins de Barros shows to be incomplete, because is unable to explain the trajectories composed of two oscillating functions observed in uncultivated magnetotactic bacteria.

Boundary behaviours of Leishmania mexicana: A hydrodynamic simulation study.

It is well established that the parasites of the genus Leishmania exhibit complex surface interactions with the sandfly vector midgut epithelium, but no prior study has considered the details of their hydrodynamics. Here, the boundary behaviours of motile Leishmania mexicana promastigotes are explored in a computational study using the boundary element method, with a model flagellar beating pattern that has been identified from digital videomicroscopy. In particular a simple flagellar kinematics is observed and quantified using image processing and mode identification techniques, suggesting a simple mechanical driver for the Leishmania beat. Phase plane analysis and long-time simulation of a range of Leishmania swimming scenarios demonstrate an absence of stable boundary motility for an idealised model promastigote, with behaviours ranging from boundary capture to deflection into the bulk both with and without surface forces between the swimmer and the boundary. Indeed, the inclusion of a short-range repulsive surface force results in the deflection of all surface-bound promastigotes, suggesting that the documented surface detachment of infective metacyclic promastigotes may be the result of their particular morphology and simple hydrodynamics. Further, simulation elucidates a remarkable morphology-dependent hydrodynamic mechanism of boundary approach, hypothesised to be the cause of the well-established phenomenon of tip-first epithelial attachment of Leishmania promastigotes to the sandfly vector midgut.

Development of Swimming Abilities in Squid Paralarvae: Behavioral and Ecological Implications for Dispersal.

This study investigates the development of swimming abilities and its relationship with morphology, growth, and nourishment of reared Doryteuthis opalescens paralarvae from hatching to 60 days of age. Paralarvae (2.5-11 mm mantle length - ML) were videotaped, and their behavior quantified throughout development using computerized motion analysis. Hatchlings swim dispersed maintaining large nearest neighbor distances (NND, 8.7 ML), with swimming speeds (SS) of 3-8 mm s-1 and paths with long horizontal displacements, resulting in high net to gross displacement ratios (NGDR). For 15-day-old paralarvae, swimming paths are more consistent between jets, growth of fins, length, and mass increases. The swimming pattern of 18-day-old paralarvae starved for 72 h exhibited a significant reduction in mean SS and inability to perform escape jets. A key morphological, behavioral, and ecological transition occurs at about 6 mm ML (>35-day old), when there is a clear change in body shape, swimming performance, and behavior, paths are more regularly repeated and directional swimming is evident, suggesting that morphological changes incur in swimming performance. These squid are able to perform sustained swimming and hover against a current at significantly closer NND (2.0 ML), as path displacement is reduced and maneuverability increases. As paralarvae reach 6-7 mm ML, they are able to attain speeds up to 562 mm s-1 and to form schools. Social feeding interactions (kleptoparasitism) are often observed prior to the formation of schools. Schools are always formed within areas of high flow gradient in the tanks and are dependent on squid size and current speed. Fin development is a requisite for synchronized and maneuverable swimming of schooling early juveniles. Although average speeds of paralarvae are within intermediate Reynolds numbers (Re < 100), they make the transition to the inertia-dominated realm during escape jets of high propulsion (Re > 3200), transitioning from plankton to nekton after their first month of life. The progressive development of swimming capabilities and social interactions enable juvenile squid to school, while also accelerates learning, orientation and cognition. These observations indicate that modeling of the lifecycle should include competency to exert influence over small currents and dispersal patterns after the first month of life.

Preparation of nanodispersions by solvent displacement using the Venturi tube.

The Venturi tube (VT) is an apparatus that produces turbulence which is taken advantage of to produce nanoparticles (NP) by solvent displacement. The objective of this study was to evaluate the potential of this device for preparing NP of poly-ε-caprolactone. Response Surface Methodology was used to determine the effect of the operating conditions and optimization. The NP produced by VT were characterized by Dynamic Light-Scattering to determine their particle size distribution (PS) and polydispersity index (PDI). Results showed that the Reynolds number (Re) has a strong effect on both PS and process yield (PY).The turbulence regime is key to the efficient formation of NP. The optimal conditions for obtaining NP were a polymer concentration of 1.6 w/v, a recirculation rate of 4.8 L/min, and a stabilizer concentration of 1.1 w/v. The predicted response of the PY was 99.7%, with a PS of 333 nm, and a PDI of 0.2. Maintaining the same preparation conditions will make it possible to obtain NP using other polymers with similar properties. Our results show that VT is a reproducible and versatile method for manufacturing NP, and so may be a feasible method for industrial-scale nanoprecipitation production.

Avaliação experimental e modelagem matemática de filtros anaeróbios como alternativa de baixo custo para remoção de algas de efluentes de lagoas de estabilização / Experimental evaluation and mathematical modeling of anaerobic filter as a low-cost alternative for algae removal from waste stabilization ponds

RESUMO Nesta pesquisa, estudou-se o desempenho de três filtros biológicos anaeróbios com novos e diferentes meios de suporte em tempos de detenção hidráulica (TDH) distintos (5,5 e 11,1 horas), objetivando a remoção de algas de efluentes de lagoas de estabilização. O considerável desempenho no pós-tratamento (remoções de até 77,4%) pode ser atribuído à atuação dos mecanismos de sedimentação, retenção física e assimilação biológica. Foi desenvolvido um modelo de decaimento para clorofila com base na simplificação da equação de transporte de massa. O coeficiente global de decaimento foi descrito como uma função dos números de Reynolds e Weber, obtida por meio de ajuste aos dados experimentais com um coeficiente de determinação superior a 0,96. Para confirmação de que a função de Reynolds e Weber descreve bem o comportamento de k, foram realizadas simulações do impacto das seguintes variáveis na eficiência de remoção de algas: comprimento do filtro (altura do leito), diâmetro do filtro, diâmetro do meio suporte, porosidade, vazão, tensão superficial e da viscosidade do líquido. As simulações desenvolvidas demonstraram coerência nos resultados, reforçando que os números de Reynolds e Weber, assim como o modelo de fluxo em pistão, podem ser usados para descrever o processo de remoção de algas em filtros anaeróbios.

ABSTRACT In this research we studied the performance of three anaerobic biological filters with new and different support media in different hydraulic retention time (HRT): 5.5 and 11.1 hours, aiming at the removal of wastewater stabilization ponds of algae. The considerable performance in the post-treatment (removal of up to 77.4%) can be attributed to the actions of sedimentation mechanisms, physical restraint and biological assimilation. It has developed a model for chlorophyll decay from the simplification of mass transport equation. The overall decay coefficient was described as a function of Reynolds and Weber numbers, which was obtained through adjusting the experimental data with a determination coefficient greater than 0.96. To confirm that the Reynolds and Weber function describes well the k behavior, simulations were made of the impact of these variables on algae removal efficiency: filter length (bed height), filter diameter, the diameter of the support means, porosity, flow, surface tension and viscosity of the liquid. Simulations show consistency in the results, reinforcing that the Reynolds and Weber numbers and the flow model of piston may be used to describe the process of removing algae in anaerobic filters.

Turbulence in microfluidics: Cleanroom-free, fast, solventless, and bondless fabrication and application in high throughput liquid-liquid extraction.

This paper addresses an important breakthrough in the deployment of ultra-high adhesion strength microfluidic technologies to provide turbulence at harsh flow rate conditions. This paper is only, to our knowledge, the second reporting on the generation of high flow rate-assisted turbulence in microchannels. This flow solves a crucial bottleneck in microfluidics: the generation of high throughput homogeneous mixings. We focused on the fabrication of bulky polydimethylsiloxane (PDMS) microchips (without any interfaces) rather than the laborious surface modifications that were employed in the first reporting about turbulence-assisted microfluidics. The fabrication is cleanroom-free, simple, low-cost, fast, solventless, and bondless requiring only a laboratory oven. More specifically, our method relies on the shaping of a nylon scaffold, cure of PDMS with embedded nylon, and removal of this scaffold. The scaffold was obtained by manually wrapping nylon threads. The withdrawing out of the scaffold was completed in few seconds using only a plier. Such microchannels endured flow rates of up to 60.0 mL min(-1) with a strikingly low elastic deformation. The importance in producing turbulence into microscale channels was successfully shown in liquid-liquid extractions. The great energy dissipation rate relative to the turbulence created high throughput and efficient extractions in microfluidics for the first time. The residence time was only 0.01 s at 25.0 mL min(-1) (total flow rate of the immiscible phases). In addition, the partition coefficient determined in a single run was similar to that obtained by the conventional batch shake-flask method that was realized in triplicate.