Secretion of collagen I and tenascin is modulated by laminin-111 in 3D culture of human adenoid cystic carcinoma cells.

Adenoid cystic carcinoma is a frequent malignant salivary gland neoplasm with high levels of recurrence and metastasis. This neoplasm expresses prominent extracellular matrix (ECM). We are studying regulatory mechanisms underlying secretion of ECM molecules in adenoid cystic carcinoma. We have previously demonstrated that laminin modulates the phenotype of a human adenoid cystic carcinoma (CAC2) cell line. Thus, this molecule would be a good candidate to regulate secretion of ECM molecules in these cells. Here we analysed the role played by laminin-111 [formerly laminin-1; Aumailley et al. (2005). Matrix Biol. 24, 326] stimulating secretory activity of CAC2 cells. Three-dimensional cultures of cells in laminin-111 (treated) or agarose (controls) were studied by light and electron microscopy. Ultrastructural analysis of CAC2 cells grown within laminin-111 showed pseudocysts filled with secretory-like material. Cells exhibited prominent and dilated endoplasmic reticulum and coated and uncoated vesicles. Ultrastructural findings suggested that laminin-111 induced secretory activity in CAC2 cells. We further investigated this point by light microscopy, immunofluorescence and confocal microscopy. Histochemistry showed periodic acid-Schiff (PAS)-positive diastase-resistant material in CAC2 cells treated by laminin-111. This material could represent laminin-induced secretion of ECM molecules. We searched for collagen I and tenascin in CAC2 cells treated by laminin-111. Confocal microscopy and immunoblot showed that laminin-111 enhanced secretion of collagen I and tenascin in CAC2 cells. We suggest that laminin-111 modulates secretion of collagen I and tenascin in cells derived from human adenoid cystic carcinoma.

Brefeldin-A induces apoptosis in human adenoid cystic carcinoma cultured cells.

Adenoid cystic carcinoma (ACC) of salivary glands is characterized by a high rate of local recurrences, neurotropism and metastasis. ACC long-term survival rate is not promising. Thus, different chemotherapeutical approaches had been proposed for this neoplasm, including apoptosis induction by different drugs. This work evaluates the efficacy of Brefeldin-A (BFA), a potent apoptosis inducer, on ACC cultured cells (CAC2 cell line). CAC2 cells were treated with a 375 microM BFA solution in serum-free medium during 18 h. CAC2 cells grown in DMEM supplemented with 10% fetal bovine serum served as controls. Apoptotic cell recognition and counting were carried out through Hoechst staining. Transmission electron microscopy and immunofluorescence assessed the effect of BFA on CAC2 cells phenotype. Treated cultures showed a high apoptotic index presenting +/-30% of cells in evident apoptosis, when compared to controls. Apoptotic CAC2 cells also exhibited different alterations such as cytoplasmic vesicles formation and mitochondrial changes. Cultured ACC cells are strongly susceptible to apoptosis induction under BFA treatment, which may constitute a promising tool in further chemotherapeutical approaches.

Cilindromatose: atualizaçäo clínica e epidemiológica / Cylindromatosis: clinical and epidemiological up-date

A proposta deste trabalho é fornecer uma revisäo e informaçöes atualizadas sobre a síndrome conhecida como cilindromatose ou tumor em turbante. A cilindromatose é uma desordem genética autossômica dominante que possui duas formas de manifestaçäo, uma de origem hereditária e a outra esporádica. A clínica se baseia no surgimento de tumores oriundos das glândulas sudoríparas écrinas que tendem a se concentrar na cabeça, principalmente, couro cabeludo e pescoço.

[Cystic adenoidal carcinoma of the external ear]. / Carcinoma adenoide quístico de conducto auditivo externo.

Tumors of the ceruminous glands can affect the auricula and periauricular skin and should be included in the differential diagnosis of lesions of these regions. Correct management of these tumors depends on precise histopathologic classification and diagnosis of their extension. We report a case of cystic adenoid carcinoma of the external auditory canal. Signs and symptoms, histopathology, and treatment of these lesions as reported in the literature are reviewed.

Argyrophilia in nucleolar organizer regions (AgNOR) in adenoid cystic carcinoma and polymorphous low-grade adenocarcinoma of the salivary glands.

Nine cases of adenoid cystic carcinomas (ACC) and three cases of polymorphous low-grade adenocarcinomas (PLGA) of the salivary glands from the Surgical Oral Pathology Service of the University of São Paulo School of Dentistry were examined by means of the silver-staining nucleolar organizer regions (AgNOR) technique. Quantitative and dimensional analyses of NORs were carried out. Our findings suggest that solid and cribriform patterns of ACC have a higher cellular activity than that presented by tubular ACC and PLGA.