ABSTRACT
The choroid plexus (ChP) produces cerebrospinal fluid and forms an essential brain barrier. ChP tissues form in each brain ventricle, each one adopting a distinct shape, but remarkably little is known about the mechanisms underlying ChP development. Here, we show that epithelial WNT5A is crucial for determining fourth ventricle (4V) ChP morphogenesis and size in mouse. Systemic Wnt5a knockout, or forced Wnt5a overexpression beginning at embryonic day 10.5, profoundly reduced ChP size and development. However, Wnt5a expression was enriched in Foxj1-positive epithelial cells of 4V ChP plexus, and its conditional deletion in these cells affected the branched, villous morphology of the 4V ChP. We found that WNT5A was enriched in epithelial cells localized to the distal tips of 4V ChP villi, where WNT5A acted locally to activate non-canonical WNT signaling via ROR1 and ROR2 receptors. During 4V ChP development, MEIS1 bound to the proximal Wnt5a promoter, and gain- and loss-of-function approaches demonstrated that MEIS1 regulated Wnt5a expression. Collectively, our findings demonstrate a dual function of WNT5A in ChP development and identify MEIS transcription factors as upstream regulators of Wnt5a in the 4V ChP epithelium.
Subject(s)
Choroid Plexus/embryology , Epithelium/metabolism , Fourth Ventricle/embryology , Myeloid Ecotropic Viral Integration Site 1 Protein/metabolism , Wnt-5a Protein/metabolism , Animals , Brain/embryology , CRISPR-Cas Systems/genetics , Cell Line , Epithelial Cells/metabolism , Female , HEK293 Cells , Humans , Mice , Mice, Knockout , Promoter Regions, Genetic/genetics , Receptor Tyrosine Kinase-like Orphan Receptors/metabolism , Signal Transduction/physiology , Wnt-5a Protein/geneticsABSTRACT
The dorsal medial region of the developing mammalian telencephalon plays a central role in the patterning of the adjacent brain regions. This review describes the development of this specialized region of the vertebrate brain, called the cortical hem, and the formation of the various cells and structures it gives rise to, including the choroid plexus, Cajal-Retzius cells and the hippocampus. We highlight the ontogenic processes that create these different forebrain derivatives from their shared embryonic origin and discuss the key signalling pathways and molecules that influence the patterning of the cortical hem. These include BMP, Wnt, FGF and Shh signalling pathways acting with Homeobox factors to carve the medial telencephalon into district progenitor regions, which in turn give rise to the choroid plexus, dentate gyrus and hippocampus. We then link the formation of the lateral ventricle choroid plexus with embryonic and postnatal neurogenesis in the hippocampus.
Subject(s)
Choroid Plexus/embryology , Dentate Gyrus/embryology , Hedgehog Proteins/metabolism , Neurogenesis , Signal Transduction , Animals , HumansABSTRACT
The choroid plexus (ChP) in each brain ventricle produces cerebrospinal fluid (CSF) and forms the blood-CSF barrier. Here, we construct a single-cell and spatial atlas of each ChP in the developing, adult, and aged mouse brain. We delineate diverse cell types, subtypes, cell states, and expression programs in epithelial and mesenchymal cells across ages and ventricles. In the developing ChP, we predict a common progenitor pool for epithelial and neuronal cells, validated by lineage tracing. Epithelial and fibroblast cells show regionalized expression by ventricle, starting at embryonic stages and persisting with age, with a dramatic transcriptional shift with maturation, and a smaller shift in each aged cell type. With aging, epithelial cells upregulate host-defense programs, and resident macrophages upregulate interleukin-1ß (IL-1ß) signaling genes. Our atlas reveals cellular diversity, architecture and signaling across ventricles during development, maturation, and aging of the ChP-brain barrier.
Subject(s)
Choroid Plexus/embryology , Choroid Plexus/metabolism , Age Factors , Aging/physiology , Animals , Blood-Brain Barrier/metabolism , Brain/metabolism , Brain/physiology , Brain Diseases/genetics , Brain Diseases/physiopathology , Cell Differentiation/genetics , Cell Lineage/genetics , Choroid Plexus/physiology , Epithelial Cells/metabolism , Female , Male , Mice/embryology , Mice, Inbred C57BL , Signal Transduction , Single-Cell AnalysisABSTRACT
OBJECTIVE: To describe the sonographic appearance and position of the choroid plexus of the fourth ventricle (4V-CP) between 12 and 21 weeks' gestation in normal fetuses and in fetuses with Dandy-Walker malformation (DWM) or Blake's pouch cyst (BPC). METHODS: The study population comprised 90 prospectively recruited normal singleton pregnancies and 41 pregnancies identified retrospectively from our institutional database that had a suspected posterior fossa anomaly at 12-13 weeks' gestation based on the ultrasound finding of abnormal hindbrain spaces. In all cases the final diagnosis was confirmed by prenatal and/or postnatal magnetic resonance imaging or postmortem examination. All pregnancies underwent a detailed ultrasound assessment, including a dedicated examination of the posterior fossa, at 12-13 weeks, 15-16 weeks and 20-21 weeks of gestation. Two-dimensional ultrasound images of the midsagittal and coronal views of the brain through the posterior fontanelle and three-dimensional volume datasets were obtained. Multiplanar orthogonal image correlation with volume contrast imaging was used as the reference visualization mode. Two independent operators, blinded to the fetal outcome, were asked to classify the 4V-CP as visible or not visible in both normal and abnormal cases, and to assess if the 4V-CP was positioned inside or outside the cyst in fetuses with DWM and BPC. RESULTS: Of the 41 fetuses with apparently isolated cystic posterior fossa anomaly in the first trimester, eight were diagnosed with DWM, 29 were diagnosed with BPC and four were found to be normal in the second trimester. The position of the 4V-CP differed between DWM, BPC and normal cases in the first- and second-trimester ultrasound examinations. In particular, in normal fetuses, no cyst was present and, in the midsagittal and coronal planes of the posterior fossa, the 4V-CP appeared as an echogenic oval-shaped structure located inside the 4V apparently attached to the cerebellar vermis. In fetuses with DWM, the 4V-CP was not visible in the midsagittal view because it was displaced inferolaterally by the cyst. In contrast, in the coronal view of the posterior brain, the 4V-CP was visualized in all cases with DWM at 12-13 weeks, with a moderate decrease in the visualization rate at 15-16 weeks (87.5%) and at 20-21 weeks (75%). In the coronal view, the 4V-CP was classified as being outside the cyst in all DWM cases at 12-13 weeks and in 87.5% and 75% of cases at 15-16 and 20-21 weeks, respectively. In fetuses with BPC, the 4V-CP was visualized in all cases in both the midsagittal and coronal views at 12-13 weeks and in 100% and 96.6% of cases, respectively, at 15-16 weeks. In the coronal view, the 4V-CP was classified as being inside the cyst in 28 (96.6%), 27 (93.1%) and 25 (86.2%) cases at 12-13, 15-16 and 20-21 weeks, respectively. The medial segment of the 4V-CP was visualized near the inferior part of the vermis. CONCLUSIONS: Our study shows that longitudinal ultrasound assessment of the 4V-CP and its temporal changes from 12 to 21 weeks is feasible. The 4V-CP is located inside the cyst, just below the vermis, in BPC and outside the cyst, inferolaterally displaced and distant from the vermian margin, in DWM, consistent with the pathogenesis of the two conditions. The position of the 4V-CP is a useful sonographic marker that can help differentiate between DWM and BPC as early as in the first trimester of pregnancy. © 2021 International Society of Ultrasound in Obstetrics and Gynecology.
Subject(s)
Central Nervous System Cysts/diagnostic imaging , Choroid Plexus/embryology , Dandy-Walker Syndrome/diagnostic imaging , Fourth Ventricle/embryology , Ultrasonography, Prenatal/methods , Central Nervous System Cysts/embryology , Choroid Plexus/diagnostic imaging , Choroid Plexus/pathology , Cranial Fossa, Posterior/diagnostic imaging , Cranial Fossa, Posterior/embryology , Cranial Fossa, Posterior/pathology , Dandy-Walker Syndrome/embryology , Databases, Factual , Diagnosis, Differential , Early Diagnosis , Feasibility Studies , Female , Fetus/diagnostic imaging , Fetus/embryology , Fourth Ventricle/diagnostic imaging , Fourth Ventricle/pathology , Humans , Pregnancy , Pregnancy Trimester, First , Pregnancy Trimester, Second , Retrospective StudiesSubject(s)
Basal Ganglia/abnormalities , Choroid Plexus/embryology , Median Eminence/abnormalities , Nervous System Malformations/diagnostic imaging , Ultrasonography, Prenatal , Abortion, Eugenic , Basal Ganglia/diagnostic imaging , Basal Ganglia/embryology , Choroid Plexus/diagnostic imaging , Choroid Plexus Neoplasms/diagnostic imaging , Choroid Plexus Neoplasms/embryology , Cysts/diagnostic imaging , Cysts/embryology , Diagnosis, Differential , Female , Humans , Hydrocephalus/diagnostic imaging , Hydrocephalus/embryology , Median Eminence/diagnostic imaging , Median Eminence/embryology , Medical Illustration , Nervous System Malformations/embryology , PregnancyABSTRACT
The choroid plexus (ChP) is a secretory tissue that produces cerebrospinal fluid (CSF) secreted into the ventricular system. It is a monolayer of secretory, multiciliated epithelial cells derived from neuroepithelial progenitors and overlying a stroma of mesenchymal cells of mesodermal origin. Zfp423, which encodes a Kruppel-type zinc-finger transcription factor essential for cerebellar development and mutated in rare cases of cerebellar vermis hypoplasia/Joubert syndrome and other ciliopathies, is expressed in the hindbrain roof plate, from which the IV ventricle ChP arises, and, later, in mesenchymal cells, which give rise to the stroma and leptomeninges. Mouse Zfp423 mutants display a marked reduction of the hindbrain ChP (hChP), which: (1) fails to express established markers of its secretory function and genes implicated in its development and maintenance (Lmx1a and Otx2); (2) shows a perturbed expression of signaling pathways previously unexplored in hChP patterning (Wnt3); and (3) displays a lack of multiciliated epithelial cells and a profound dysregulation of master genes of multiciliogenesis (Gmnc). Our results propose that Zfp423 is a master gene and one of the earliest known determinants of hChP development.
Subject(s)
Choroid Plexus/embryology , DNA-Binding Proteins/metabolism , Rhombencephalon/embryology , Transcription Factors/metabolism , Animals , Choroid Plexus/cytology , DNA-Binding Proteins/genetics , LIM-Homeodomain Proteins/genetics , LIM-Homeodomain Proteins/metabolism , Mice , Mice, Mutant Strains , Otx Transcription Factors/genetics , Otx Transcription Factors/metabolism , Rhombencephalon/cytology , Transcription Factors/genetics , Wnt3 Protein/genetics , Wnt3 Protein/metabolismABSTRACT
The choroid plexus (ChP) regulates brain development by secreting instructive cues and providing a protective brain barrier. Here, we show that polyI:C-mediated maternal immune activation leads to an inflammatory response in the developing embryonic mouse brain that manifests as pro-inflammatory cerebrospinal fluid (CSF) and accumulation of ChP macrophages. Elevation of CSF-CCL2 was sufficient to drive ChP immune cell recruitment, activation, and proliferation. In addition, ChP macrophages abandoned their regular tiling pattern and relocated to the ChP-free margin where they breached the weakened epithelial barrier. We further found that these immune cells entered from the ChP into the brain via anatomically specialized "hotspots" at the distal tips of ChP villi. In vivo two-photon imaging demonstrated that surveillance behaviors in ChP macrophages had already emerged at this early stage of embryogenesis. Thus, the embryonic ChP forms a functional brain barrier that can mount an inflammatory response to external insults.
Subject(s)
Choroid Plexus/embryology , Choroid Plexus/immunology , Inflammation/pathology , Animals , Calcium-Binding Proteins/metabolism , Cell Proliferation , Cerebrospinal Fluid/metabolism , Chemokine CCL2/metabolism , Imaging, Three-Dimensional , Inflammation Mediators/metabolism , Macrophage Activation , Mice, Inbred C57BL , Microfilament Proteins/metabolism , Receptors, CCR2/metabolism , Signal Transduction , Tight Junctions/metabolism , Up-RegulationABSTRACT
The choroid plexus (CP) is the predominant supplier of cerebral spinal fluid (CSF) and the site of the blood-CSF barrier and is thus essential for brain development and central nervous system homeostasis. Despite these crucial roles, our understanding of the molecular and cellular processes giving rise to the CPs within the ventricles of the mammalian brain is very rudimentary. Here, we identify WNT5a as an important regulator of CP development, where it acts as a pivotal factor driving CP epithelial morphogenesis in all ventricles. We show that WNT5a is essential for the establishment of a cohesive epithelium in the developing CP. We find that in its absence all CPs are substantially reduced in size and complexity and fail to expand into the ventricles. Severe defects were observed in the epithelial cytoarchitecture of all Wnt5a-/- CPs, exemplified by loss of apicobasally polarized morphology and detachment from the ventricular surface and/or basement membrane. We also present evidence that the WNT5a receptor, RYK, and the RHOA kinase, ROCK, are required for normal CP epithelial morphogenesis. Our study, therefore, reveals important insights into the molecular and cellular mechanisms governing CP development.
Subject(s)
Choroid Plexus/embryology , Epithelial Cells/ultrastructure , Receptor Protein-Tyrosine Kinases/genetics , Wnt-5a Protein/genetics , Amides/pharmacology , Animals , Cell Shape/drug effects , Cell Shape/genetics , Choroid Plexus/cytology , Choroid Plexus/drug effects , Choroid Plexus/ultrastructure , Enzyme Inhibitors/pharmacology , Epithelial Cells/drug effects , Injections, Intraventricular , Mice , Microinjections , Microscopy, Electron, Transmission , Morphogenesis/genetics , Pyridines/pharmacology , Receptor Protein-Tyrosine Kinases/metabolism , Wnt-5a Protein/metabolism , rho-Associated Kinases/antagonists & inhibitors , rho-Associated Kinases/metabolismABSTRACT
Endosomal sorting complex required for transport (ESCRT) complex proteins regulate biogenesis and release of extracellular vesicles (EVs), which enable cell-to-cell communication in the nervous system essential for development and adult function. We recently showed human loss-of-function (LOF) mutations in ESCRT-III member CHMP1A cause autosomal recessive microcephaly with pontocerebellar hypoplasia, but its mechanism was unclear. Here, we show Chmp1a is required for progenitor proliferation in mouse cortex and cerebellum and progenitor maintenance in human cerebral organoids. In Chmp1a null mice, this defect is associated with impaired sonic hedgehog (Shh) secretion and intraluminal vesicle (ILV) formation in multivesicular bodies (MVBs). Furthermore, we show CHMP1A is important for release of an EV subtype that contains AXL, RAB18, and TMED10 (ART) and SHH. Our findings show CHMP1A loss impairs secretion of SHH on ART-EVs, providing molecular mechanistic insights into the role of ESCRT proteins and EVs in the brain.
Subject(s)
Endosomal Sorting Complexes Required for Transport/metabolism , Extracellular Vesicles/metabolism , Hedgehog Proteins/metabolism , Adult , Animals , Brain/embryology , Brain/metabolism , Choroid Plexus/embryology , Choroid Plexus/growth & development , Choroid Plexus/metabolism , Humans , Infant, Newborn , Mice , NIH 3T3 Cells , Vesicular Transport ProteinsABSTRACT
OBJECTIVES: Diagnosis of ventriculomegaly (VM) and identification of choroid plexus (CP) can be challenging with fetal magnetic resonance imaging (MRI). Our aim is to create an adjunct method for supporting the diagnosis of VM by investigating the CP-ventricular wall separation distance in fetuses with and without VM (nV) with fetal MRI. METHODS: T2-weighted fetal MRIs of 154 fetuses were retrospectively evaluated. The CP separation was defined as the distance between the medial wall of the dependent ventricle and distal tip of the CP glomus. The measurement was performed at the same plane with the dependent ventricle measurement by two blinded readers. RESULTS: 41 fetuses with VM (mean gestational age 27 (19-35â¯weeks), and 44â¯nV fetuses (mean gestational age 28 (20-39â¯weeks) were included. Interobserver reliability was excellent for ventricle diameters (Râ¯=â¯0.99, confidence interval (CI) 95%) and the separation of CP (Râ¯=â¯0.98, CI 95%). Mean distance of CP separation was 10.7â¯mm⯱â¯4.2â¯mm and 3.0⯱â¯1.6â¯mm in VM and nV fetuses, respectively (pâ¯<â¯0.001). The distance of CP separation to differentiate VM cases was 6.5â¯mm (sensitivity: 0.98, specificity: 0.98). Separation of CP was correlated to ventricle diameter in cases with (Râ¯=â¯0.674) and without VM (Râ¯=â¯0.805). For the cut-off value >0.65â¯cm for the distance between the medial wall of the dependent ventricle and the medial border of choroid plexus sensitivity is 97.56, specificity 95.45, positive predictive value (PPV) 95.20, negative predictive value (NPV) 97.70, and likelihood ratio (LR) (+) is 21.46. CONCLUSION: Fetal CP can be efficiently evaluated with MRI, and the increase of CP-ventricular wall separation distance in correlation with the ventricle diameter is a reliable sign in the diagnosis of fetal VM.
Subject(s)
Cerebral Ventricles/diagnostic imaging , Choroid Plexus/diagnostic imaging , Magnetic Resonance Imaging , Nervous System Malformations/diagnostic imaging , Prenatal Diagnosis , Area Under Curve , Cerebral Ventricles/embryology , Choroid Plexus/embryology , Humans , ROC Curve , Retrospective StudiesABSTRACT
Nervous system development is a precisely orchestrated series of events requiring a multitude of intrinsic and extrinsic cues. Sortilin and SorCS2 are members of the Vps10p receptor family with complementary influence on some of these cues including the neurotrophins (NTs). However, the developmental time points where sortilin and SorCS2 exert their activities in conjunction or independently still remain unclear. In this study we present the characterization of the spatiotemporal expression pattern of sortilin and SorCS2 in the developing murine nervous system. Sortilin is highly expressed in the fetal nervous system with expression localized to distinct cell populations. Expression was high in neurons of the cortical plate and developing allocortex, as well as subpallial structures. Furthermore, the neuroepithelium lining the ventricles and the choroid plexus showed high expression of sortilin, together with the developing retina, spinal ganglia, and sympathetic ganglia. In contrast, SorCS2 was confined in a marked degree to the thalamus and, at E13.5, the floor plate from midbrain rostrally to spinal cord caudally. SorCS2 was also found in the ventricular zones of the ventral hippocampus and nucleus accumbens areas, in the meninges and in Schwann cells. Hence, sortilin and SorCS2 are extensively present in several distinct anatomical areas in the developing nervous system and are rarely co-expressed. Possible functions of sortilin and SorCS2 pertain to NT signaling, axon guidance and beyond. The present data will form the basis for hypotheses and study designs for unravelling the functions of sortilin and SorCS2 during the establishment of neuronal structures and connections.
Subject(s)
Adaptor Proteins, Vesicular Transport/metabolism , Central Nervous System , Gene Expression Regulation, Developmental/physiology , Nerve Tissue Proteins/metabolism , Peripheral Nerves , Receptors, Cell Surface/metabolism , Adaptor Proteins, Vesicular Transport/genetics , Animals , Animals, Newborn , Calbindin 2/metabolism , Calbindins/metabolism , Central Nervous System/embryology , Central Nervous System/growth & development , Central Nervous System/metabolism , Choroid Plexus/embryology , Choroid Plexus/growth & development , Choroid Plexus/metabolism , Embryo, Mammalian , Ependyma/embryology , Ependyma/growth & development , Ependyma/metabolism , Meninges/embryology , Meninges/growth & development , Meninges/metabolism , Mice , Mice, Inbred C57BL , Mice, Transgenic , Nerve Tissue Proteins/genetics , Peripheral Nerves/embryology , Peripheral Nerves/growth & development , Peripheral Nerves/metabolism , Receptors, Cell Surface/genetics , Tyrosine 3-Monooxygenase/metabolismABSTRACT
Pediatric hydrocephalus is characterized by an abnormal accumulation of cerebrospinal fluid (CSF) and is one of the most common congenital brain abnormalities. However, little is known about the molecular and cellular mechanisms regulating CSF flow in the developing brain. Through whole-genome sequencing analysis, we report that a homozygous splice site mutation in coiled-coil domain containing 39 (Ccdc39) is responsible for early postnatal hydrocephalus in the progressive hydrocephalus (prh) mouse mutant. Ccdc39 is selectively expressed in embryonic choroid plexus and ependymal cells on the medial wall of the forebrain ventricle, and the protein is localized to the axoneme of motile cilia. The Ccdc39prh/prh ependymal cells develop shorter cilia with disorganized microtubules lacking the axonemal inner arm dynein. Using high-speed video microscopy, we show that an orchestrated ependymal ciliary beating pattern controls unidirectional CSF flow on the ventricular surface, which generates bulk CSF flow in the developing brain. Collectively, our data provide the first evidence for involvement of Ccdc39 in hydrocephalus and suggest that the proper development of medial wall ependymal cilia is crucial for normal mouse brain development.
Subject(s)
Choroid Plexus , Cytoskeletal Proteins , Ependyma , Gene Expression Regulation, Developmental , Hydrocephalus , Animals , Choroid Plexus/embryology , Choroid Plexus/pathology , Cilia/genetics , Cilia/metabolism , Cytoskeletal Proteins/genetics , Cytoskeletal Proteins/metabolism , Ependyma/embryology , Ependyma/pathology , Hydrocephalus/embryology , Hydrocephalus/genetics , Hydrocephalus/pathology , Mice , Mice, Mutant StrainsABSTRACT
OBJECTIVE: To assess non-visualization of the choroid plexus of the fourth ventricle (CP-4V) as a simple, qualitative and reproducible first-trimester ultrasound feature of the posterior fossa for the prediction of central nervous system (CNS) anomalies and chromosomal defects. METHODS: First-trimester three-dimensional ultrasound datasets of the fetal brain were obtained prospectively from 65 consecutive normal singletons and retrospectively from 27 fetuses identified as having an abnormal posterior fossa on first-trimester ultrasound examination, and randomly combined to form the final study group. The stored ultrasound volumes were analyzed offline by two accredited sonologists, who were not aware of the final diagnoses. The CP-4V was assessed by multiplanar navigation and classified as visible or non-visible in its normal position depending on whether or not the echogenic structure that separates the fourth ventricle from the cisterna magna was identified in both midsagittal and axial planes. Correlation with subsequent second-trimester ultrasound, fetal magnetic resonance imaging, or postmortem or postnatal findings was performed to determine the predictive value of the first-trimester findings. RESULTS: Among the 92 ultrasound datasets analyzed, 73 (79%) were acquired transabdominally and 19 (21%) transvaginally. The CP-4V was classified as visible in 64 cases and non-visible in 28 cases, with agreement between the two observers in both sagittal and axial planes in all but one case. Twelve of the 28 (43%) fetuses with non-visible CP-4V were subsequently diagnosed as having a CNS malformation (open spina bifida (n = 6), Dandy-Walker malformation (n = 2), Blake's pouch cyst (n = 2), cephalocele (n = 1) and megacisterna magna (n = 1)). In addition, 20 of these 28 (71%) fetuses had aneuploidy (trisomy 18 (n = 10), triploidy (n = 5), trisomy 13 (n = 3), Turner syndrome (n = 1) or trisomy 21 (n = 1)). There was only one false-positive case, in which the CP-4V was classified as absent in a normal fetus. CONCLUSIONS: Non-visualization of the CP-4V in the first trimester appears to be a strong marker of posterior fossa anomalies and chromosomal defects. Qualitative evaluation of this anatomic structure is simple, feasible and reproducible, and its routine assessment during the first-trimester scan may facilitate the early detection of CNS anomalies and associated fetal aneuploidy. Copyright © 2017 ISUOG. Published by John Wiley & Sons Ltd.
Subject(s)
Choroid Plexus/diagnostic imaging , Cisterna Magna/embryology , Cranial Fossa, Posterior/abnormalities , Fetus/abnormalities , Fourth Ventricle/diagnostic imaging , Nervous System Malformations/diagnostic imaging , Ultrasonography, Prenatal , Adult , Choroid Plexus/embryology , Cisterna Magna/diagnostic imaging , Cranial Fossa, Posterior/diagnostic imaging , Echocardiography, Three-Dimensional , Female , Fourth Ventricle/embryology , Gestational Age , Humans , Nervous System Malformations/embryology , Nervous System Malformations/physiopathology , Observer Variation , Predictive Value of Tests , Pregnancy , Pregnancy Trimester, First , Prospective Studies , Reference Values , Reproducibility of Results , Retrospective Studies , SpainABSTRACT
The choroid plexus (ChP) is a non-neural epithelial tissue that produces cerebrospinal fluid (CSF). The ChP differentiates from the roof plate, a dorsal midline structure of the neural tube. However, molecular mechanisms underlying ChP development are poorly understood compared to neural development. MafB is a bZip transcription factor that is known to be expressed in the roof plate. Here we investigated the role of MafB in embryonic development of the hindbrain ChP (hChP) using Mafb-deficient mice. Immunohistochemical analyses revealed that MafB is expressed in the roof plate and early hChP epithelial cells but its expression disappears at a later embryonic stage. We also found that the Mafb-deficient hChP exhibits delayed differentiation and results in hypoplasia compared to the wild-type hChP. Furthermore, the Mafb-deficient hChP exhibits increased apoptotic cell death and decreased proliferating cells at E12.5, an early stage of hChP development. Collectively, our findings reveal that MafB play an important role in promoting hChP development during embryogenesis.
Subject(s)
Choroid Plexus/embryology , Gene Expression Regulation, Developmental , MafB Transcription Factor/genetics , MafB Transcription Factor/physiology , Rhombencephalon/embryology , Animals , Apoptosis , Cell Differentiation , Cell Proliferation , Immunohistochemistry , In Situ Hybridization , LIM-Homeodomain Proteins/genetics , LIM-Homeodomain Proteins/metabolism , Mice , Mice, Transgenic , Neural Tube/metabolism , Signal Transduction , Transcription Factors/genetics , Transcription Factors/metabolismABSTRACT
OBJECTIVE: To describe the sonographic appearance and temporal changes of the structures of the posterior cranial fossa in fetuses at a crown-rump length (CRL) between 45 and 84 mm in transvaginal acquired three-dimensional volume blocks. METHODS: This was a prospective, cross-sectional, observational study including 80 fetuses, whose mothers attended Kepler University Hospital Linz or the Ambulatorium für Fetalmedizin Feldkirch for first-trimester sonography. Three-dimensional volume blocks were acquired in a standardized way and after processing the sonographic characteristics of the brainstem, cerebellar vermis, choroid plexus, anterior membranous area (AMA) and Blake's metapore were described. Measurements of the length of the cerebellar vermis, the length of the AMA and the medulla-oblongata-pons angle (MOPA) were performed. In 20 fetuses the intra- and interobserver repeatability was calculated. RESULTS: The sonomorphologic characteristics of posterior fossa structures as cerebellar vermis, AMA, Blake's metapore, choroid plexus, pons and medulla oblongata were described. There is a significant correlation between CRL and vermis length, CRL and MOPA and CRL and AMA. CONCLUSIONS: Transvaginal three-dimensional sonography allows a detailed depiction of the structures of the posterior fossa and their temporal course in early pregnancy. © 2016 John Wiley & Sons, Ltd.
Subject(s)
Brain Stem/diagnostic imaging , Choroid Plexus/diagnostic imaging , Cranial Fossa, Posterior/diagnostic imaging , Adolescent , Adult , Brain Stem/embryology , Cerebellar Vermis/diagnostic imaging , Cerebellar Vermis/embryology , Choroid Plexus/embryology , Cranial Fossa, Posterior/embryology , Cross-Sectional Studies , Crown-Rump Length , Female , Gestational Age , Humans , Imaging, Three-Dimensional , Medulla Oblongata/diagnostic imaging , Medulla Oblongata/embryology , Pons/diagnostic imaging , Pons/embryology , Pregnancy , Pregnancy Trimester, First , Reproducibility of Results , Ultrasonography, Prenatal , Young AdultABSTRACT
BACKGROUND: The aim of the study was to report the prevalence and associated findings of fetal ventriculomegaly between 11 + 0 and 13 + 6 gestational weeks and to evaluate a sonographic approach to classify first trimester ventriculomegaly in the standard axial plane used for biparietal diameter (BPD) measurement. METHODS: The ratio between choroid plexus and lateral ventricle diameter (PDVDR), between the choroid plexus and lateral ventricle length (PLVLR) and between the choroid plexus and lateral ventricle area (PAVAR) were calculated from stored 2D images of the axial head plane in 100 normal fetuses and 17 fetuses with ventriculomegaly. RESULTS: The PDVDR, the PLVLR and the PAVAR were below the 5(th) percentile in 82.4%, 94.1% and 94.1% of the cases with ventriculomegaly. Ventriculomegaly was isolated in 29.4% and associated with further anomalies in 70.6% at the initial evaluation. The mean PLVLR in euploid compared to aneuploid fetuses was significantly lower (0.40 versus 0.53 (p = 0.0332)). CONCLUSIONS: The measurements of PDVDR, PLVLR and PAVAR are helpful to objectify ventriculomegaly at 11-14 gestational weeks. The PLVLR and PAVAR were superior to PDVDR, since there seems to be rather shrinkage of the choroid plexus than an increased width of the lateral ventricles in the first trimester.
Subject(s)
Hydrocephalus/diagnostic imaging , Pregnancy Trimester, First , Ultrasonography, Prenatal/methods , Choroid Plexus/diagnostic imaging , Choroid Plexus/embryology , Female , Gestational Age , Head/diagnostic imaging , Head/embryology , Humans , Lateral Ventricles/diagnostic imaging , Lateral Ventricles/embryology , PregnancyABSTRACT
The developing dorsomedial telencephalon includes the medial pallium, which goes on to form the hippocampus. Generating a reliable source of human hippocampal tissue is an important step for cell-based research into hippocampus-related diseases. Here we show the generation of functional hippocampal granule- and pyramidal-like neurons from self-organizing dorsomedial telencephalic tissue using human embryonic stem cells (hESCs). First, we develop a hESC culture method that utilizes bone morphogenetic protein (BMP) and Wnt signalling to induce choroid plexus, the most dorsomedial portion of the telencephalon. Then, we find that titrating BMP and Wnt exposure allowed the self-organization of medial pallium tissues. Following long-term dissociation culture, these dorsomedial telencephalic tissues give rise to Zbtb20(+)/Prox1(+) granule neurons and Zbtb20(+)/KA1(+) pyramidal neurons, both of which were electrically functional with network formation. Thus, we have developed an in vitro model that recapitulates human hippocampus development, allowing the generation of functional hippocampal granule- and pyramidal-like neurons.
Subject(s)
Choroid Plexus/embryology , Hippocampus/embryology , Human Embryonic Stem Cells/cytology , Pyramidal Cells/cytology , Telencephalon/embryology , Bone Morphogenetic Proteins/pharmacology , Cell Culture Techniques , Cell Differentiation/drug effects , Choroid Plexus/drug effects , Hippocampus/drug effects , Human Embryonic Stem Cells/drug effects , Humans , Immunohistochemistry , In Vitro Techniques , Neurons/cytology , Neurons/drug effects , Patch-Clamp Techniques , Polymerase Chain Reaction , Telencephalon/drug effects , Wnt Proteins/pharmacology , Wnt Signaling PathwayABSTRACT
PURPOSE: To evaluate fetuses with choroid plexus separation without ventriculomegaly in terms of fetal malformations, behavior of the separation during follow-up, and postnatal outcome. METHODS: In total, 172 fetuses with choroid plexus separation without ventriculomegaly were included in this prospective study. Fetal sonography was performed at 2- to 4-week intervals, and detailed physical and neurologic examinations were performed after their delivery. Fetuses were categorized into normal and abnormal subgroups according to the outcome. RESULTS: Sixteen fetuses (9.3%) were included in the abnormal-outcome group and 156 fetuses (90.7%) were included in the normal-outcome group. Both the initial mean lateral ventricular diameter (9.3 mm versus 8.6 mm) and the initial mean choroid plexus separation (4.8 mm versus 3.3 mm) were greater in the abnormal group than in the normal group (p < 0.001 for both comparisons). We found that 4.0 mm was the best cutoff point of choroid plexus separation to detect a major anomaly, with 87.5% sensitivity and 93.6% specificity. CONCLUSIONS: Choroid plexus separation without ventriculomegaly often resolves within the third trimester and does not affect postnatal outcome. It can be associated with various fetal malformations; however, with a comprehensive examination, all fetal malformations can be detected prenatally. Follow-up sonography studies would be useful, especially in the case of suspected corpus callosum agenesis.
Subject(s)
Cerebral Ventricles/diagnostic imaging , Cerebral Ventricles/embryology , Choroid Plexus/diagnostic imaging , Choroid Plexus/embryology , Ultrasonography, Prenatal , Adult , Brain Diseases/diagnostic imaging , Female , Fetal Diseases/diagnostic imaging , Humans , Infant , Infant, Newborn , Pregnancy , Pregnancy Outcome , Pregnancy Trimester, Second , Prospective Studies , Sensitivity and SpecificityABSTRACT
The developing forebrain roof plate (RP) contains a transient signaling center, perturbations in which have been linked to holoprosencephaly (HPE). Here, we describe a novel domain of retinoic acid (RA) signaling that is specific to the chick RP and demonstrate that RA signaling is sufficient for inducing characteristics of the RP in ectopic locations. We further demonstrate that, unlike what has been observed in the mouse, RA signaling is essential for invagination of the RP in chick, failure of which leads to an HPE-like phenotype. In addition, we found that RA exerts a negative influence on choroid plexus differentiation. Thus, our findings identify RA as a novel regulator of chick forebrain RP development.