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1.
Cell Chem Biol ; 31(1): 139-149.e14, 2024 01 18.
Article En | MEDLINE | ID: mdl-37967558

A novel class of benzoxaboroles was reported to induce cancer cell death but the mechanism was unknown. Using a forward genetics platform, we discovered mutations in cleavage and polyadenylation specific factor 3 (CPSF3) that reduce benzoxaborole binding and confer resistance. CPSF3 is the endonuclease responsible for pre-mRNA 3'-end processing, which is also important for RNA polymerase II transcription termination. Benzoxaboroles inhibit this endonuclease activity of CPSF3 in vitro and also curb transcriptional termination in cells, which results in the downregulation of numerous constitutively expressed genes. Furthermore, we used X-ray crystallography to demonstrate that benzoxaboroles bind to the active site of CPSF3 in a manner distinct from the other known inhibitors of CPSF3. The benzoxaborole compound impeded the growth of cancer cell lines derived from different lineages. Our results suggest benzoxaboroles may represent a promising lead as CPSF3 inhibitors for clinical development.


Antineoplastic Agents , Boron Compounds , Cleavage And Polyadenylation Specificity Factor , Endonucleases , RNA Precursors , RNA Processing, Post-Transcriptional , Cleavage And Polyadenylation Specificity Factor/antagonists & inhibitors , Cleavage And Polyadenylation Specificity Factor/chemistry , Endonucleases/antagonists & inhibitors , RNA Precursors/genetics , RNA Precursors/metabolism , Boron Compounds/chemistry , Boron Compounds/pharmacology , Antineoplastic Agents/chemistry , Antineoplastic Agents/pharmacology , RNA Processing, Post-Transcriptional/drug effects , Humans , Cell Line, Tumor
2.
Open Biol ; 13(11): 230221, 2023 Nov.
Article En | MEDLINE | ID: mdl-37989222

Eukaryotic pre-mRNA is processed by a large multiprotein complex to accurately cleave the 3' end, and to catalyse the addition of the poly(A) tail. Within this cleavage and polyadenylation specificity factor (CPSF) machinery, the CPSF73/CPSF3 endonuclease subunit directly contacts both CPSF100/CPSF2 and the scaffold protein Symplekin to form a subcomplex known as the core cleavage complex or mammalian cleavage factor. Here we have taken advantage of a stable CPSF73-CPSF100 minimal heterodimer from Encephalitozoon cuniculi to determine the solution structure formed by the first and second C-terminal domain (CTD1 and CTD2) of both proteins. We find a large number of contacts between both proteins in the complex, and notably in the region between CTD1 and CTD2. A similarity is also observed between CTD2 and the TATA-box binding protein (TBP) domains. Separately, we have determined the structure of the terminal CTD3 domain of CPSF73, which also belongs to the TBP domain family and is connected by a flexible linker to the rest of CPSF73. Biochemical assays demonstrate a key role for the CTD3 of CPSF73 in binding Symplekin, and structural models of the trimeric complex from other species allow for comparative analysis and support an overall conserved architecture.


Cleavage And Polyadenylation Specificity Factor , Encephalitozoon cuniculi , mRNA Cleavage and Polyadenylation Factors , Cleavage And Polyadenylation Specificity Factor/genetics , Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/metabolism , mRNA Cleavage and Polyadenylation Factors/genetics
3.
J Biol Chem ; 299(4): 103047, 2023 04.
Article En | MEDLINE | ID: mdl-36822327

Human cleavage and polyadenylation specificity factor (CPSF)73 (also known as CPSF3) is the endoribonuclease that catalyzes the cleavage reaction for the 3'-end processing of pre-mRNAs. The active site of CPSF73 is located at the interface between a metallo-ß-lactamase domain and a ß-CASP domain. Two metal ions are coordinated by conserved residues, five His and two Asp, in the active site, and they are critical for the nuclease reaction. The metal ions have long been thought to be zinc ions, but their exact identity has not been examined. Here we present evidence from inductively coupled plasma mass spectrometry and X-ray diffraction analyses that a mixture of metal ions, including Fe, Zn, and Mn, is present in the active site of CPSF73. The abundance of the various metal ions is different in samples prepared from different expression hosts. Zinc is present at less than 20% abundance in a sample expressed in insect cells, but the sample is active in cleaving a pre-mRNA substrate in a reconstituted canonical 3'-end processing machinery. Zinc is present at 75% abundance in a sample expressed in human cells, which has comparable endonuclease activity. We also observe a mixture of metal ions in the active site of the CPSF73 homolog INTS11, the endonuclease for Integrator. Taken together, our results provide further insights into the role of metal ions in the activity of CPSF73 and INTS11 for RNA 3'-end processing.


Cleavage And Polyadenylation Specificity Factor , Endonucleases , Humans , Catalytic Domain , Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/metabolism , Endonucleases/chemistry , Endonucleases/metabolism , RNA Processing, Post-Transcriptional , Zinc/metabolism
4.
Biomol NMR Assign ; 17(1): 43-48, 2023 06.
Article En | MEDLINE | ID: mdl-36723825

The initial pre-mRNA transcript in eukaryotes is processed by a large multi-protein complex in order to correctly cleave the 3' end, and to subsequently add the polyadenosine tail. This cleavage and polyadenylation specificity factor (CPSF) is composed of separate subunits, with structural information available for both isolated subunits and also larger assembled complexes. Nevertheless, certain key components of CPSF still lack high-resolution atomic data. One such region is the heterodimer formed between the first and second C-terminal domains of the endonuclease CPSF73, with those from the catalytically inactive CPSF100. Here we report the backbone and sidechain resonance assignments of a minimal C-terminal heterodimer of CPSF73-CPSF100 derived from the parasite Encephalitozoon cuniculi. The assignment process used several amino-acid specific labeling strategies, and the chemical shift values allow for secondary structure prediction.


Cleavage And Polyadenylation Specificity Factor , RNA 3' End Processing , Nuclear Magnetic Resonance, Biomolecular , Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/genetics , Cleavage And Polyadenylation Specificity Factor/metabolism , RNA Precursors/metabolism , mRNA Cleavage and Polyadenylation Factors/genetics
5.
RNA ; 27(10): 1148-1154, 2021 10.
Article En | MEDLINE | ID: mdl-34230059

CPSF73 is the endonuclease that catalyzes the cleavage reaction for 3'-end processing of mRNA precursors (pre-mRNAs) in two distinct machineries, a canonical machinery for the majority of pre-mRNAs and a U7 snRNP (U7 machinery) for replication-dependent histone pre-mRNAs in animal cells. CPSF73 also possesses 5'-3' exonuclease activity in the U7 machinery, degrading the downstream cleavage product after the endonucleolytic cleavage. Recent studies show that CPSF73 is a potential target for developing anticancer, antimalarial, and antiprotozoal drugs, spurring interest in identifying new small-molecule inhibitors against this enzyme. CPSF73 nuclease activity has so far been demonstrated using a gel-based end-point assay, using radiolabeled or fluorescently labeled RNA substrates. By taking advantage of unique properties of the U7 machinery, we have developed a novel, real-time fluorescence assay for the nuclease activity of CPSF73. This assay is facile and high-throughput, and should also be helpful for the discovery of new CPSF73 inhibitors.


Biological Assay , Cleavage And Polyadenylation Specificity Factor/metabolism , Histones/metabolism , RNA 3' End Processing , RNA Precursors/metabolism , Ribonucleoprotein, U7 Small Nuclear/metabolism , Cell-Free System , Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/genetics , Enzyme Assays , Enzyme Inhibitors/chemistry , Enzyme Inhibitors/pharmacology , Fluorescence , Fluorescent Dyes/chemistry , Histones/chemistry , Histones/genetics , Humans , Models, Molecular , Phenylalanine/analogs & derivatives , Phenylalanine/chemistry , Phenylalanine/pharmacology , Piperazines/chemistry , Piperazines/pharmacology , Proteolysis , RNA Precursors/chemistry , RNA Precursors/genetics , Rhodamines/chemistry , Ribonucleoprotein, U7 Small Nuclear/chemistry , Ribonucleoprotein, U7 Small Nuclear/genetics , Small Molecule Libraries/chemistry , Small Molecule Libraries/pharmacology
6.
Biochemistry ; 60(10): 780-790, 2021 03 16.
Article En | MEDLINE | ID: mdl-33615774

Cleavage and polyadenylation specificity factor 30 (CPSF30) is a zinc finger protein that regulates pre-mRNA processing. CPSF30 contains five CCCH domains and one CCHC domain and recognizes two conserved 3' pre-mRNA sequences: an AU hexamer and a U-rich motif. AU hexamer motifs are common in pre-mRNAs and are typically defined as AAUAAA. Variations within the AAUAAA hexamer occur in certain pre-mRNAs and can affect polyadenylation efficiency or be linked to diseases. The effects of disease-related variations on CPSF30/pre-mRNA binding were determined using a construct of CPSF30 that contains just the five CCCH domains (CPSF30-5F). Bioinformatics was utilized to identify the variability within the AU hexamer sequence in pre-mRNAs. The effects of this sequence variability on CPSF30-5F/RNA binding affinities were measured. Bases at positions 1, 2, 4, and 5 within the AU hexamer were found to be important for RNA binding. Bioinformatics revealed that the three bases flanking the AU hexamer at the 5' and 3' ends are twice as likely to be adenine or uracil as guanine and cytosine. The presence of A and U residues in these flanking regions was determined to promote higher-affinity CPSF30-5F/RNA binding than G and C residues. The addition of the zinc knuckle domain to CPSF30-5F (CPSF30-FL) restored binding to AU hexamer variants. This restoration of binding is connected to the presence of a U-rich sequence within the pre-mRNA to which the zinc knuckle binds. A mechanism of differential RNA binding by CPSF30, modulated by accessibility of the two RNA binding sites, is proposed.


Cleavage And Polyadenylation Specificity Factor/metabolism , Poly U/metabolism , Polyadenylation , RNA Precursors/metabolism , RNA, Messenger/metabolism , Animals , Binding Sites , Cattle , Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/genetics , Protein Binding , RNA Precursors/genetics , RNA, Messenger/chemistry , RNA, Messenger/genetics , Zinc Fingers
7.
Genes Dev ; 34(23-24): 1753-1761, 2020 12 01.
Article En | MEDLINE | ID: mdl-33122294

Most eukaryotic pre-mRNAs must undergo 3'-end cleavage and polyadenylation prior to their export from the nucleus. A large number of proteins in several complexes participate in this 3'-end processing, including cleavage and polyadenylation specificity factor (CPSF) in mammals. The CPSF30 subunit contains five CCCH zinc fingers (ZFs), with ZF2-ZF3 being required for the recognition of the AAUAAA poly(A) signal. ZF4-ZF5 recruits the hFip1 subunit of CPSF, although the details of this interaction have not been characterized. Here we report the crystal structure of human CPSF30 ZF4-ZF5 in complex with residues 161-200 of hFip1 at 1.9 Å resolution, illuminating the molecular basis for their interaction. Unexpectedly, the structure reveals one hFip1 molecule binding to each ZF4 and ZF5, with a conserved mode of interaction. Our mutagenesis studies confirm that the CPSF30-hFip1 complex has 1:2 stoichiometry in vitro. Mutation of each binding site in CPSF30 still allows one copy of hFip1 to bind, while mutation of both sites abrogates binding. Our fluorescence polarization binding assays show that ZF4 has higher affinity for hFip1, with a Kd of 1.8 nM. We also demonstrate that two copies of the catalytic module of poly(A) polymerase (PAP) are recruited by the CPSF30-hFip1 complex in vitro, and both hFip1 binding sites in CPSF30 can support polyadenylation.


Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/metabolism , Models, Molecular , mRNA Cleavage and Polyadenylation Factors/chemistry , Binding Sites , Cleavage And Polyadenylation Specificity Factor/genetics , Escherichia coli/genetics , Humans , Mutation , Protein Binding , Protein Structure, Quaternary , Zinc Fingers/physiology , mRNA Cleavage and Polyadenylation Factors/genetics , mRNA Cleavage and Polyadenylation Factors/metabolism
8.
Nat Microbiol ; 5(4): 545-553, 2020 04.
Article En | MEDLINE | ID: mdl-32094586

Regulated gene expression is largely achieved by controlling the activities of essential, multisubunit RNA polymerase transcription elongation complexes (TECs). The extreme stability required of TECs to processively transcribe large genomic regions necessitates robust mechanisms to terminate transcription. Efficient transcription termination is particularly critical for gene-dense bacterial and archaeal genomes1-3 in which continued transcription would necessarily transcribe immediately adjacent genes and result in conflicts between the transcription and replication apparatuses4-6; the coupling of transcription and translation7,8 would permit the loading of ribosomes onto aberrant transcripts. Only select sequences or transcription termination factors can disrupt the otherwise extremely stable TEC and we demonstrate that one of the last universally conserved archaeal proteins with unknown biological function is the Factor that terminates transcription in Archaea (FttA). FttA resolves the dichotomy of a prokaryotic gene structure (operons and polarity) and eukaryotic molecular homology (general transcription apparatus) that is observed in Archaea. This missing link between prokaryotic and eukaryotic transcription regulation provides the most parsimonious link to the evolution of the processing activities involved in RNA 3'-end formation in Eukarya.


Archaeal Proteins/chemistry , Cleavage And Polyadenylation Specificity Factor/chemistry , Genome, Archaeal , Thermococcus/genetics , Transcription Factors/chemistry , Transcription Termination, Genetic , Archaeal Proteins/genetics , Archaeal Proteins/metabolism , Bacteria/genetics , Bacteria/metabolism , Biological Evolution , Cleavage And Polyadenylation Specificity Factor/genetics , Cleavage And Polyadenylation Specificity Factor/metabolism , DNA-Directed RNA Polymerases/genetics , DNA-Directed RNA Polymerases/metabolism , Humans , Models, Molecular , Protein Biosynthesis , RNA, Messenger/genetics , RNA, Messenger/metabolism , Structural Homology, Protein , Thermococcus/metabolism , Transcription Elongation, Genetic , Transcription Factors/genetics , Transcription Factors/metabolism , Transcription Initiation, Genetic
9.
Science ; 367(6478): 700-703, 2020 02 07.
Article En | MEDLINE | ID: mdl-32029631

The 3'-end processing machinery for metazoan replication-dependent histone precursor messenger RNAs (pre-mRNAs) contains the U7 small nuclear ribonucleoprotein and shares the key cleavage module with the canonical cleavage and polyadenylation machinery. We reconstituted an active human histone pre-mRNA processing machinery using 13 recombinant proteins and two RNAs and determined its structure by cryo-electron microscopy. The overall structure is highly asymmetrical and resembles an amphora with one long handle. We captured the pre-mRNA in the active site of the endonuclease, the 73-kilodalton subunit of the cleavage and polyadenylation specificity factor, poised for cleavage. The endonuclease and the entire cleavage module undergo extensive rearrangements for activation, triggered through the recognition of the duplex between the authentic pre-mRNA and U7 small nuclear RNA (snRNA). Our study also has notable implications for understanding canonical and snRNA 3'-end processing.


Cleavage And Polyadenylation Specificity Factor/chemistry , Histones/genetics , RNA Cleavage , RNA Precursors/metabolism , Catalytic Domain , Cryoelectron Microscopy , Humans , Polyadenylation , RNA, Small Nuclear/metabolism , Recombinant Proteins , Ribonucleoprotein, U7 Small Nuclear/chemistry
10.
Biochemistry ; 59(8): 970-982, 2020 03 03.
Article En | MEDLINE | ID: mdl-32027124

Cleavage and polyadenylation specificity factor 30 (CPSF30) is a "zinc finger" protein that plays a crucial role in the transition of pre-mRNA to RNA. CPSF30 contains five conserved CCCH domains and a CCHC "zinc knuckle" domain. CPSF30 activity is critical for pre-mRNA processing. A truncated form of the protein, in which only the CCCH domains are present, has been shown to specifically bind AU-rich pre-mRNA targets; however, the RNA binding and recognition properties of full-length CPSF30 are not known. Herein, we report the isolation and biochemical characterization of full-length CPSF30. We report that CPSF30 contains one 2Fe-2S cluster in addition to five zinc ions, as measured by inductively coupled plasma mass spectrometry, ultraviolet-visible spectroscopy, and X-ray absorption spectroscopy. Utilizing fluorescence anisotropy RNA binding assays, we show that full-length CPSF30 has high binding affinity for two types of pre-mRNA targets, AAUAAA and polyU, both of which are conserved sequence motifs present in the majority of pre-mRNAs. Binding to the AAUAAA motif requires that the five CCCH domains of CPSF30 be present, whereas binding to polyU sequences requires the entire, full-length CPSF30. These findings implicate the CCHC "zinc knuckle" present in the full-length protein as being critical for mediating polyU binding. We also report that truncated forms of the protein, containing either just two CCCH domains (ZF2 and ZF3) or the CCHC "zinc knuckle" domain, do not exhibit any RNA binding, indicating that CPSF30/RNA binding requires several ZF (and/or Fe-S cluster) domains working in concert to mediate RNA recognition.


Cleavage And Polyadenylation Specificity Factor/metabolism , Iron-Sulfur Proteins/metabolism , Poly U/metabolism , RNA Precursors/metabolism , Amino Acid Sequence , Animals , Cattle , Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/genetics , Cobalt/chemistry , Iron-Sulfur Proteins/chemistry , Iron-Sulfur Proteins/genetics , Mutation , Protein Binding , RNA Precursors/genetics , Zinc/chemistry , Zinc Fingers , alpha-Synuclein/genetics
11.
J Eukaryot Microbiol ; 67(3): 306-320, 2020 05.
Article En | MEDLINE | ID: mdl-31898347

In humans, mRNA polyadenylation involves the participation of about 20 factors in four main complexes that recognize specific RNA sequences. Notably, CFIm25, CPSF73, and PAP have essential roles for poly(A) site selection, mRNA cleavage, and adenosine residues polymerization. Besides the relevance of polyadenylation for gene expression, information is scarce in intestinal protozoan parasites that threaten human health. To better understand polyadenylation in Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum, which represent leading causes of diarrhea worldwide, genomes were screened for orthologs of human factors. Results showed that Entamoeba histolytica and C. parvum have 16 and 12 proteins out of the 19 human proteins used as queries, respectively, while G. lamblia seems to have the smallest polyadenylation machinery with only six factors. Remarkably, CPSF30, CPSF73, CstF77, PABP2, and PAP, which were found in all parasites, could represent the core polyadenylation machinery. Multiple genes were detected for several proteins in Entamoeba, while gene redundancy is lower in Giardia and Cryptosporidium. Congruently with their relevance in the polyadenylation process, CPSF73 and PAP are present in all parasites, and CFIm25 is only missing in Giardia. They conserve the functional domains and predicted folding of human proteins, suggesting they may have the same roles in polyadenylation.


Cleavage And Polyadenylation Specificity Factor/genetics , Cryptosporidium parvum/genetics , Entamoeba histolytica/genetics , Giardia lamblia/genetics , Intestines/parasitology , RNA, Messenger/genetics , Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/metabolism , Cryptosporidium parvum/metabolism , Databases, Genetic , Entamoeba histolytica/metabolism , Giardia lamblia/metabolism , Humans , Models, Molecular , Open Reading Frames , Poly A/chemistry , Protein Domains , Protein Structure, Tertiary , Protozoan Proteins/chemistry , Protozoan Proteins/genetics , Protozoan Proteins/metabolism , RNA, Messenger/chemistry , RNA, Protozoan/chemistry , RNA, Protozoan/genetics , Sequence Alignment , Sequence Analysis, Protein
12.
Mol Cell ; 77(4): 800-809.e6, 2020 02 20.
Article En | MEDLINE | ID: mdl-31810758

The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core.


Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/genetics , Cleavage Stimulation Factor/chemistry , Cryoelectron Microscopy , Humans , Models, Molecular , Mutation , RNA 3' End Processing , RNA Precursors/metabolism , RNA, Messenger/metabolism
13.
Sci Transl Med ; 11(517)2019 11 06.
Article En | MEDLINE | ID: mdl-31694928

Cryptosporidium is an intestinal pathogen that causes severe but self-limiting diarrhea in healthy humans, yet it can turn into a life-threatening, unrelenting infection in immunocompromised patients and young children. Severe diarrhea is recognized as the leading cause of mortality for children below 5 years of age in developing countries. The only approved treatment against cryptosporidiosis, nitazoxanide, has limited efficacy in the most vulnerable patient populations, including malnourished children, and is ineffective in immunocompromised individuals. Here, we investigate inhibition of the parasitic cleavage and polyadenylation specificity factor 3 (CPSF3) as a strategy to control Cryptosporidium infection. We show that the oxaborole AN3661 selectively blocked Cryptosporidium growth in human HCT-8 cells, and oral treatment with AN3661 reduced intestinal parasite burden in both immunocompromised and neonatal mouse models of infection with greater efficacy than nitazoxanide. Furthermore, we present crystal structures of recombinantly produced Cryptosporidium CPSF3, revealing a mechanism of action whereby the mRNA processing activity of this enzyme is efficiently blocked by the binding of the oxaborole group at the metal-dependent catalytic center. Our data provide insights that may help accelerate the development of next-generation anti-Cryptosporidium therapeutics.


Cleavage And Polyadenylation Specificity Factor/metabolism , Cryptosporidiosis/genetics , Cryptosporidiosis/parasitology , Cryptosporidium/genetics , Metals/chemistry , RNA Precursors/genetics , RNA Processing, Post-Transcriptional , Animals , Antiparasitic Agents/chemistry , Antiparasitic Agents/pharmacology , Cell Line, Tumor , Cleavage And Polyadenylation Specificity Factor/chemistry , Crystallization , Humans , Ileum/parasitology , Ileum/ultrastructure , Mice, Inbred C57BL , Models, Molecular , Recombinant Proteins/metabolism
14.
Biochimie ; 164: 105-110, 2019 Sep.
Article En | MEDLINE | ID: mdl-30930282

Pre-mRNA 3'-end maturation is achieved by a mechanism requiring four different protein complexes assembled from approximately twenty factors. A global understanding of this essential process is still missing due to the inability to structurally characterize the entire complexes, even though structures of the isolated factors have been obtained. In this review, we summarize recent findings regarding the atomic description of one of the major players, the Cleavage and Polyadenylation Specificity Factor complex (CPSF in human, CPF in yeast). These data provide information on the architecture adopted by the major components of this complex, and on its capacity to recognize the polyadenylation signal sequence.


Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage Stimulation Factor/chemistry , RNA, Messenger/metabolism , Fungal Proteins/chemistry , Humans , Polyadenylation , Protein Binding , Yeasts/genetics , Yeasts/metabolism
15.
Methods Enzymol ; 599: 101-137, 2018.
Article En | MEDLINE | ID: mdl-29746237

Zinc finger (ZF) proteins are proteins that use zinc as a structural cofactor. The common feature among all ZFs is that they contain repeats of four cysteine and/or histidine residues within their primary amino acid sequence. With the explosion of genome sequencing in the early 2000s, a large number of proteins were annotated as ZFs based solely upon amino acid sequence. As these proteins began to be characterized experimentally, it was discovered that some of these proteins contain iron-sulfur sites either in place of or in addition to zinc. Here, we describe methods to isolate and characterize one such ZF protein, cleavage and polyadenylation specificity factor 30 (CPSF3O) with respect to its metal-loading and RNA-binding activity.


Cleavage And Polyadenylation Specificity Factor/chemistry , Cleavage And Polyadenylation Specificity Factor/metabolism , RNA/metabolism , Zinc Fingers , Animals , Chromatography, Gel/methods , Cleavage And Polyadenylation Specificity Factor/genetics , Cleavage And Polyadenylation Specificity Factor/isolation & purification , Cloning, Molecular/methods , Electrophoretic Mobility Shift Assay/methods , Escherichia coli/genetics , Fluorescence Polarization/methods , Humans , Iron-Sulfur Proteins/chemistry , Iron-Sulfur Proteins/genetics , Iron-Sulfur Proteins/isolation & purification , Iron-Sulfur Proteins/metabolism , Mass Spectrometry/methods , Models, Molecular , X-Ray Absorption Spectroscopy/methods
16.
Nat Struct Mol Biol ; 25(2): 135-138, 2018 02.
Article En | MEDLINE | ID: mdl-29358758

Mammalian mRNA biogenesis requires specific recognition of a hexanucleotide AAUAAA motif in the polyadenylation signals (PAS) of precursor mRNA (pre-mRNA) transcripts by the cleavage and polyadenylation specificity factor (CPSF) complex. Here we present a 3.1-Å-resolution cryo-EM structure of a core CPSF module bound to the PAS hexamer motif. The structure reveals the molecular interactions responsible for base-specific recognition, providing a rationale for mechanistic differences between mammalian and yeast 3' polyadenylation.


Cleavage And Polyadenylation Specificity Factor/chemistry , Polyadenylation , RNA Precursors/chemistry , Amino Acid Motifs , Cryoelectron Microscopy , Humans , Image Processing, Computer-Assisted , Molecular Structure , Motion , Nuclear Proteins/chemistry , Poly A/chemistry , Protein Binding , Protein Domains , Protein Multimerization , RNA, Messenger/chemistry
17.
Proc Natl Acad Sci U S A ; 115(7): E1419-E1428, 2018 02 13.
Article En | MEDLINE | ID: mdl-29208711

Nearly all eukaryotic messenger RNA precursors must undergo cleavage and polyadenylation at their 3'-end for maturation. A crucial step in this process is the recognition of the AAUAAA polyadenylation signal (PAS), and the molecular mechanism of this recognition has been a long-standing problem. Here, we report the cryo-electron microscopy structure of a quaternary complex of human CPSF-160, WDR33, CPSF-30, and an AAUAAA RNA at 3.4-Å resolution. Strikingly, the AAUAAA PAS assumes an unusual conformation that allows this short motif to be bound directly by both CPSF-30 and WDR33. The A1 and A2 bases are recognized specifically by zinc finger 2 (ZF2) of CPSF-30 and the A4 and A5 bases by ZF3. Interestingly, the U3 and A6 bases form an intramolecular Hoogsteen base pair and directly contact WDR33. CPSF-160 functions as an essential scaffold and preorganizes CPSF-30 and WDR33 for high-affinity binding to AAUAAA. Our findings provide an elegant molecular explanation for how PAS sequences are recognized for mRNA 3'-end formation.


Cleavage And Polyadenylation Specificity Factor/metabolism , Nuclear Proteins/metabolism , Poly A/metabolism , Polyadenylation , RNA Precursors/metabolism , RNA, Messenger/metabolism , Cleavage And Polyadenylation Specificity Factor/chemistry , Cryoelectron Microscopy , Humans , Models, Molecular , Nuclear Proteins/chemistry , Poly A/chemistry , Protein Conformation , RNA Precursors/chemistry , RNA, Messenger/chemistry
18.
Elife ; 62017 12 23.
Article En | MEDLINE | ID: mdl-29274231

3' polyadenylation is a key step in eukaryotic mRNA biogenesis. In mammalian cells, this process is dependent on the recognition of the hexanucleotide AAUAAA motif in the pre-mRNA polyadenylation signal by the cleavage and polyadenylation specificity factor (CPSF) complex. A core CPSF complex comprising CPSF160, WDR33, CPSF30 and Fip1 is sufficient for AAUAAA motif recognition, yet the molecular interactions underpinning its assembly and mechanism of PAS recognition are not understood. Based on cross-linking-coupled mass spectrometry, crystal structure of the CPSF160-WDR33 subcomplex and biochemical assays, we define the molecular architecture of the core human CPSF complex, identifying specific domains involved in inter-subunit interactions. In addition to zinc finger domains in CPSF30, we identify using quantitative RNA-binding assays an N-terminal lysine/arginine-rich motif in WDR33 as a critical determinant of specific AAUAAA motif recognition. Together, these results shed light on the function of CPSF in mediating PAS-dependent RNA cleavage and polyadenylation.


Cleavage And Polyadenylation Specificity Factor/metabolism , Nuclear Proteins/metabolism , RNA Precursors/metabolism , mRNA Cleavage and Polyadenylation Factors/metabolism , Cleavage And Polyadenylation Specificity Factor/chemistry , Crystallography, X-Ray , Humans , Hydrolysis , Mass Spectrometry , Nuclear Proteins/chemistry , Polyadenylation , Protein Binding , Protein Interaction Domains and Motifs , mRNA Cleavage and Polyadenylation Factors/chemistry
19.
Nat Commun ; 8: 14574, 2017 03 06.
Article En | MEDLINE | ID: mdl-28262680

Benzoxaboroles are effective against bacterial, fungal and protozoan pathogens. We report potent activity of the benzoxaborole AN3661 against Plasmodium falciparum laboratory-adapted strains (mean IC50 32 nM), Ugandan field isolates (mean ex vivo IC50 64 nM), and murine P. berghei and P. falciparum infections (day 4 ED90 0.34 and 0.57 mg kg-1, respectively). Multiple P. falciparum lines selected in vitro for resistance to AN3661 harboured point mutations in pfcpsf3, which encodes a homologue of mammalian cleavage and polyadenylation specificity factor subunit 3 (CPSF-73 or CPSF3). CRISPR-Cas9-mediated introduction of pfcpsf3 mutations into parental lines recapitulated AN3661 resistance. PfCPSF3 homology models placed these mutations in the active site, where AN3661 is predicted to bind. Transcripts for three trophozoite-expressed genes were lost in AN3661-treated trophozoites, which was not observed in parasites selected or engineered for AN3661 resistance. Our results identify the pre-mRNA processing factor PfCPSF3 as a promising antimalarial drug target.


Antimalarials/pharmacology , Boron Compounds/pharmacology , Cleavage And Polyadenylation Specificity Factor/chemistry , Plasmodium falciparum/drug effects , Protozoan Proteins/chemistry , RNA, Messenger/genetics , Amino Acid Sequence , Animals , Antimalarials/chemical synthesis , Boron Compounds/chemical synthesis , CRISPR-Cas Systems , Catalytic Domain , Cleavage And Polyadenylation Specificity Factor/antagonists & inhibitors , Cleavage And Polyadenylation Specificity Factor/genetics , Cleavage And Polyadenylation Specificity Factor/metabolism , Drug Resistance/genetics , Erythrocytes/drug effects , Erythrocytes/parasitology , Gene Editing/methods , Humans , Malaria/drug therapy , Malaria/parasitology , Malaria, Falciparum/drug therapy , Malaria, Falciparum/parasitology , Mice , Molecular Docking Simulation , Mutation , Plasmodium berghei/drug effects , Plasmodium berghei/genetics , Plasmodium berghei/growth & development , Plasmodium berghei/metabolism , Plasmodium falciparum/genetics , Plasmodium falciparum/growth & development , Plasmodium falciparum/metabolism , Protein Binding , Protein Interaction Domains and Motifs , Protein Structure, Secondary , Protozoan Proteins/antagonists & inhibitors , Protozoan Proteins/genetics , Protozoan Proteins/metabolism , RNA, Messenger/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Trophozoites/drug effects , Trophozoites/genetics , Trophozoites/growth & development , Trophozoites/metabolism
20.
Proc Natl Acad Sci U S A ; 113(17): 4700-5, 2016 Apr 26.
Article En | MEDLINE | ID: mdl-27071088

Cleavage and polyadenylation specificity factor 30 (CPSF30) is a key protein involved in pre-mRNA processing. CPSF30 contains five Cys3His domains (annotated as "zinc-finger" domains). Using inductively coupled plasma mass spectrometry, X-ray absorption spectroscopy, and UV-visible spectroscopy, we report that CPSF30 is isolated with iron, in addition to zinc. Iron is present in CPSF30 as a 2Fe-2S cluster and uses one of the Cys3His domains; 2Fe-2S clusters with a Cys3His ligand set are rare and notably have also been identified in MitoNEET, a protein that was also annotated as a zinc finger. These findings support a role for iron in some zinc-finger proteins. Using electrophoretic mobility shift assays and fluorescence anisotropy, we report that CPSF30 selectively recognizes the AU-rich hexamer (AAUAAA) sequence present in pre-mRNA, providing the first molecular-based evidence to our knowledge for CPSF30/RNA binding. Removal of zinc, or both zinc and iron, abrogates binding, whereas removal of just iron significantly lessens binding. From these data we propose a model for RNA recognition that involves a metal-dependent cooperative binding mechanism.


Cleavage And Polyadenylation Specificity Factor/chemistry , Iron/chemistry , RNA 3' Polyadenylation Signals/genetics , RNA, Messenger/chemistry , Sulfur/chemistry , mRNA Cleavage and Polyadenylation Factors/chemistry , Binding Sites , Cleavage And Polyadenylation Specificity Factor/genetics , Computer Simulation , Humans , Models, Chemical , Polyadenylation/genetics , Protein Binding , mRNA Cleavage and Polyadenylation Factors/genetics
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