Distribution of various volumes of methylene blue injection in suprainguinal fascia iliaca block under ultrasound guidance: a cadaveric study / Distribución de varios volúmenes de inyección de azul de metileno en el bloque suprainguinal de la fascia ilíaca bajo guía por ultrasonido: un estudio cadavérico

SUMMARY: The local anesthetic volume for a single-shot suprainguinal fascia iliaca block (SFIB) is a key factor of a block success because the courses of the three target nerves from the lumbar plexus (LP), the lateral femoral cutaneous nerve (LFCN), femoral nerve (FN), and obturator nerve (ON), at the inguinal area are isolated and within striking distance. Thus, this cadaveric study aims to demonstrate the distribution of dye staining on the LFCN, FN, ON, and LP following the ultrasound-guided SFIB using 15-50 ml of methylene blue. A total of 40 USG-SFIBs were performed on 20 fresh adult cadavers using 15, 20, 25, 30, 35, 40, 45, and 50 ml of methylene blue. After the injections, the pelvic and inguinal regions were dissected to directly visualize the dye stained on the LFCN, FN, ON, and LP. All FN and LFCN were stained heavily when the 15-50 ml of dye was injected. Higher volumes of dye (40-50 ml) spread more medially and stained on the ON and LP in 60 % of cases. To increase the possibility of dye spreading to all three target nerves and LP of the SFIB, a high volume (≥40 ml) of anesthetic is recommended. If only a blockade of the FN and LFCN is required, a low volume (15-25 ml) of anesthetic is sufficient.

RESUMEN: El volumen de anestésico local para un bloqueo de la fascia ilíaca suprainguinal (FISI) de una sola inyección es un factor clave para el éxito del bloqueo, debido a que los cursos de los tres nervios objetivo del plexo lumbar (PL), el nervio cutáneo femoral lateral (NCFL), femoral (NF) y el nervio obturador (NO), en el área inguinal están aislados y dentro de la distancia de abordaje. Por lo tanto, este estudio cadavérico tiene como objetivo demostrar la distribución de la tinción de tinte en NCFL, NF, NO y PL siguiendo el FISI guiado por ultrasonido usando 15-50 ml de azul de metileno. Se realizaron un total de 40 USG-FISI en 20 cadáveres adultos frescos utilizando 15, 20, 25, 30, 35, 40, 45 y 50 ml de azul de metileno. Después de las inyecciones, se disecaron las regiones pélvica e inguinal para visualizar directamente el tinte teñido en NCFL, NF, NO y PL. Todos los NF y NCFL se tiñeron intensamente cuando se inyectaron los 15- 50 ml de colorante. Volúmenes mayores de colorante (40-50 ml) se esparcen más medialmente y tiñen el NO y la PL en el 60 % de los casos. Para aumentar la posibilidad de que el colorante se propague a los tres nervios objetivo y al PL del FISI, se recomienda un volumen elevado (≥40 ml) de anestésico. Si solo se requiere un bloqueo de NF y NCFL, un volumen bajo (15-25 ml) de anestésico es suficiente.

Understanding the anatomy of the Denonvilliers fascia: review article / Compreendendo a anatomia da fáscia de Denonvilliers: artigo de revisão

Abstract The postoperative outcome of rectal cancer has been improved after the introduction of the principles of total mesorectal excision (TME). Total mesorectal excision includes resection of the diseased rectum and mesorectum with non-violated mesorectal fascia (en bloc resection). Dissection along themesorectal fascia through the principle of the "holy plane" minimizes injury of the autonomic nerves and increases the chance of preserving them. It is important to stick to the TME principle to avoid perforating the tumor; violating the mesorectal fascia, thus resulting in positive circumferential resection margin (CRM); or causing injury to the autonomic nerves, especially if the tumor is located anteriorly. Therefore, identifying the anterior plane of dissection during TME is important because it is related with the autonomic nerves (Denonvilliers fascia). Although there are many articles about the Denonvilliers fascia (DVF) or the anterior dissection plane, unfortunately, there is no consensus on its embryological origin, histology, and gross anatomy. In the present review article, I aim to delineate and describe the anatomy of the DVF inmore details based on a review of the literature, in order to provide insight for colorectal surgeons to better understand this anatomical feature and to provide the best care to their patients.

Resumo O resultado pós-operatório do câncer retal foi melhorado após a introdução dos princípios da excisão total do mesorreto (TME, na sigla em inglês). A excisão total do mesorreto inclui a ressecção do reto e do mesorreto afetados com fáscia mesorretal não violada (ressecção em bloco). A dissecção ao longo da fáscia mesorretal pelo princípio do "plano sagrado" minimiza a lesão dos nervos autônomos e aumenta a chance de preservá-los. É importante seguir o princípio da TME para evitar: a perfuração do tumor; a violação da fáscia mesorretal, resultando em margem de ressecção circunferencial (CRM) positiva; ou a lesão aos nervos autônomos, especialmente se o tumor estiver localizado anteriormente. Portanto, a identificação do plano anterior de dissecção durante a TME é importante, pois está relacionada comos nervos autonômicos (fáscia de Denonvilliers). Embora existammuitos artigos sobre a fáscia de Denonvilliers (DVF, na sigla em inglês) ou o plano de dissecção anterior, infelizmente não há consenso sobre sua origem embriológica, histologia e anatomia macroscópica. No presente artigo de revisão, retendo delinear e descrever a anatomia da DVF em mais detalhes com base em uma revisão da literatura, a fim de fornecer subsídios para os cirurgiões colorretais entenderemmelhor esta característica anatômica e fornecer o melhor cuidado para seus pacientes.

Relaciones entre las fascias endopelvianas y el plexo hipogástrico inferior / Relationship between the endopelvic fascia and the inferior hypogastric plexus

El Plexo Hipogástrico Inferior (PHI) es un plexo difícil de definir y disecar, de allí la facilidad con que puede lesionarse tanto en la investigación anatómica como quirúrgica. Definir sus relaciones, con respecto a las fascias endopelvianas (FEP), incluyendo su formación y sus ramos, (Baader et al., 2003, p. 129)facilitaría su disección. Esta investigación anatómica pretende estandarizar Se utilizó material cadavérico perteneciente a la Tercera Cátedra de Anatomía de la Facultad de Medicina de la Universidad de Buenos Aires. Se disecaron un bloque de órganos de cadáver adulto formolizado (n=1) y diecisiete (n = 17) hemipelvis: cinco (n = 5) hemipelvis masculinas adultas formolizadas, nueve (n = 9) hemipelvis fetales formalizadas (7 masculinos y 2 femeninas), entre 18 y 36 semanas de edad gestacional calculada por la longitud femoral y tres (n = 3) hemipelvis adultas de cadáveres frescos, dos (n = 2) femeninas y un (n = 1) masculino. Se utilizaron elementos de microdisección y lupas.Pudimos distinguir tres sectores diferentes: el primero, preplexual, ubicado posterior y lateralmente a la FEP, donde los componentes simpáticos (nervios hipogástricos) y los parasimpáticos (nervios esplácnicos pélvicos) aún no han confluido para su formación. Un segundo sector, plexual, con el plexo ya completamente formado, ubicado en el espesor de la FEP. Por último, su porción terminal, ya desprovisto de la FEP, formado por nervios que se dirigen a la membrana perineal acompañados por vasos arteriales y venosos. Cada uno de estos sectores requiere distinto abordaje tanto en la disección anatómica como quirúrgica.

The Inferior Hypogastric Plexus (PHI) is a difficult plexus to define and dissect, hence the ease with which it can be injured both in anatomical and surgical research. Defining its relationships, with respect to the endopelvic fascia (FEP), including its formation and branches, (Baader et al., 2003, p. 129) would facilitate their dissection. This anatomical investigation aims to standardize different portions that require a different approach to preserve their integrity.Cadaveric material belonging to the Third Chair of Anatomy of the School of Medicine, Buenos Aires University was used. One (n=1) formolized male adult organ block and seventeen (n=17) hemipelvis were dissected: five (n=5) adult male hemipelvis formolized, nine (n=9) fetal hemipelvis formolized (7 male and 2 female), between 18 and 36 weeks of gestational age calculated by femoral length, and three (n=3) adult hemipelvis from fresh cadavers, two (n=2) female and one (n=1) male. Microdissection elements and magnifying glasses were used. We were able to distinguish three different sectors: the first, preplexual, located posterior and lateral to the FEP, where the sympathetic components (hypogastric nerves) and the parasympathetic (pelvic splanchnic nerves) have not yet converged to form the plexus. A second sector, plexual, with the plexus already fully formed, located in the thickness of the FEP. Finally, its terminal portion, already devoid of the FEP, formed by nerves that go to the perineal membrane accompanied by arterial and venous vessels. Each of these sectors requires a different approach in both anatomical and surgical dissection.

Anatomical and physiological considerations in the 'hip rotators test' related to the stomatognathic system: a brief commentary / Consideraciones anatomofisiológicas en el "test de rotadores de cadera" relacionado con el sistema estomatognático: un breve comentario

Manual tests in clinical investigation must be supported by anatomical and physiological findings in order to obtain an objective information. The application of different mandibular positions in children obtains a variation in the 'hip rotators test' (p < 0.001). The possible relationships behind the muscle tone of the external rotators of the hips and the stomatognathic system are exposed, with special attention on the fascial tissue and its morphological characteristics. Despite these anatomical and physiological connections, there is no further evidence of a strong cause-effect relationship in this test.

Las pruebas manuales en la investigación clínica deben estar respaldadas por hallazgos anatómicos y fisiológicos para obtener una información objetiva. La aplicación de diferentes posiciones mandibulares en niños muestra una variación en la "prueba de rotadores de cadera" (p <0,001). Se exponen las posibles relaciones del tono muscular de los rotadores externos de las caderas y el sistema estomatognático, con especial atención en el tejido fascial y sus características morfológicas. A pesar de estas conexiones anatomofisiológicas, no existe una evidencia mayor de una relación importante causa-efecto en esta prueba.

Uso de fáscia peitoral maior em preenchimento de dorso nasal: relato de caso / Use of pectoralis major fascia in dorsal nasal augmentation: case report

O aumento do dorso nasal nas rinoplastias é foco de estudo de diversos trabalhos que buscam as melhores fontes de enxerto e técnicas cirúrgicas. A utilização de cartilagem já é consagrada para este fim, a partir do septo nasal, da concha auricular ou dos arcos costais. Nos últimos anos, têm-se buscado meios para reduzir a palpabilidade e dispersibilidade dos enxertos cartilaginosos. Assim, são descritos materiais sintéticos, como o SURGICEL®; e, autólogos, representados pelas fáscias. A fáscia temporal é mais amplamente utilizada, porém requer uma nova incisão cirúrgica, aumentando o tempo e a morbidade da cirurgia. É também descrito o uso de fáscia lata e fáscia reto abdominal, comparativamente mais espessas e menos flexíveis. Em muitos casos de rinoplastia fazse necessária a retirada da cartilagem costal, o que permite a coleta de fáscia do músculo peitoral maior pela mesma incisão cirúrgica. Dessa forma, descrevemos a utilização da fáscia do músculo peitoral maior envolvendo cartilagem costal picada, em uma rinoplastia estruturada com aumento do dorso.

Increasing the nasal dorsum in rhinoplasty is the focus of several studies that seek the best graft sources and surgical techniques. The use of cartilage from the nasal septum, ear shell, or costal arches is already established for this purpose. In recent years, methods have been sought to reduce the palpability and dispersibility of cartilaginous grafts. Thus, synthetic materials such as SURGICEL® and autologous materials such as fascia have been explored. Temporal fascia are more widely used but require a new surgical incision, increasing surgical time and morbidity. Also described is the use of fascia lata and rectus abdominis fascia, which are comparatively thicker and less flexible. In many rhinoplasty procedures, it is necessary to remove the costal cartilage, which allows the collection of fascia from the major chest muscles through the same surgical incision. Thus, we describe the use of major chest muscle fascia and chopped costal cartilage in structured rhinoplasty to increase the dorsum.

The plantar aponeurosis in fetuses and adults: an aponeurosis or fascia? / La aponeurosis plantar en fetos y adultos: ¿aponeurosis o fascia?

The plantar aponeurosis (PA), which is a thickened layer of deep fascia located on the plantar surface of the foot, is comprised of three parts. There are differing opinions on its nomenclature since various authors use the terms PA and plantar fascia (PF) interchangeably. In addition, the variable classifications of its parts has led to confusion. In order to assess the nature of the PA, this study documented its morphology. Furthermore, a pilot histological analysis was conducted to examine whether the structure is an aponeurosis or fascia. This study comprised of a morphological analysis of the three parts of the PA by micro- and macro-dissection of 50 fetal and 50 adult cadaveric feet, respectively (total n=100). Furthermore, a pilot histological analysis was conducted on five fetuses (n=10) and five adults (n=10) (total n=20). In each foot, the histological analysis was conducted on the three parts of the plantar aponeurosis, i.e. the central, lateral, and medial at their calcaneal origin (total n=60). Fetuses: i) Morphology: In 66 % (33/50) of the specimens, the standard anatomical pattern was observed, viz. three parts (i.e. central, lateral, medial) that originated from the medial and lateral processes of the calcaneal tuberosity and inserted onto the metatarsals. In 18 % (9/50) of the specimens, a two-part structure was observed. Variable origins of the medial part were noted in 16 % (8/50) of the specimens. In order to document these variations, the central part of the PA was divided into three segments (i.e. upper, middle, lower): a) In 63 % (5/8) of the specimens, the medial part arose from the middle segment; b) In 37 % (3/8) of the specimens, the medial part arose from the middle and upper segments. ii) Histological analysis: a) The central part contained longitudinally arranged semi-dense type I collagen fibres with fibroblasts; b) The lateral part displayed semi-dense type I collagen fibres with fibroblasts, hyaluronic acid, corpusculum sensorium fusiforme (Ruffini corpuscle) and corpusculum lamellosum (Pacinian corpuscle); c) The medial part comprised of loose connective tissue with elastic and reticular fibres. Adults: i) Morphology: In 100 % of the specimens, the standard anatomical pattern was observed. ii) Histological Analysis: a) In the central part, longitudinally arranged type I collagen fibres with fibroblasts were visible; b) The lateral part contained longitudinally arranged type I collagen fibres with fibroblasts; c) The medial part comprised of loose connective tissue, type I and type III collagen fibres, elastic and reticular fibres. In the current study, the morphology of the PA in fetuses and adults conformed to the standard anatomical description with variations in the origin of the medial part observed in fetuses. In addition, the fetal specimens displayed a two-part structure of the PA when the medial part was absent. Microscopically, the findings suggest that only the central and lateral parts may be considered as the PA, whilst the medial part may be termed the PF.

La aponeurosis plantar (AP), que es una capa engrosada de fascia profunda localizada en la superficie plantar del pie, está compuesta de tres partes. Hay diferentes opiniones sobre su nomenclatura, ya que varios autores utilizan los términos AP y fascia plantar (FP) de forma intercambiable. Además, las distintas clasificaciones de sus partes han dado lugar a confusión. Con el fin de evaluar la naturaleza de la AP, este estudio documentó su morfología. Además, se realizó un análisis histológico para examinar si la estructura es una aponeurosis o fascia. Este estudio consistió en un análisis morfológico de las tres partes de la AP de 50 pies de fetos y 50 pies de cadáveres adultos, por micro y macrodisección, respectivamente (total n = 100). Además, se realizó un análisis histológico en cinco fetos (n = 10) y cinco adultos (n = 10) (total n = 20). En cada pie, el análisis histológico se realizó sobre las tres partes de la aponeurosis plantar, es decir, la central, lateral y medial en su origen calcáneo (total n = 60). Fetos: i) Morfología: En el 66 % (33/50) de los especímenes, se observó el patrón anatómico estándar, es decir, tres partes (central, lateral y medial) que se originaron a partir de los procesos medial y lateral de la tuberosidad calcánea y se insertaban en los metatarsianos. En 18 % (9/50) de los especímenes, se observó una estructura de dos partes. Los orígenes variables de la parte mediana se visualizaron en el 16 % (8/50) de los especímenes. Para documentar estas variaciones, la parte central de la AP se dividió en tres segmentos (superior, medio, inferior): en el 63 % (5/8) de los casos, la parte mediana surgió del segmento medio; en el 37 % (3/8) de los casos, los casos la parte medial surgió de los segmentos medio y superior. ii) Análisis histológico: a) La parte central contenía fibras de colágeno tipo I semi-densas dispuestas longitudinalmente con fibroblastos; b) La parte lateral mostró fibras de colágeno tipo I semi-densas con fibroblastos, ácido hialurónico, corpúsculo sensorial fusiform (corpúsculo de Ruffini) y corpúsculo lamellosum (corpúsculo de Pacini); c) La parte medial comprende tejido conjuntivo suelto con fibras elásticas y reticulares. Adultos: i) Morfología: En el 100 % de los especímenes se observó el patrón anatómico estándar. ii) Análisis histológico: a) En la parte central, se observaron fibras de colágeno de tipo I con disposición longitudinal de fibroblastos; b) La parte lateral contenía fibras de colágeno de tipo I dispuestas longitudinalmente con fibroblastos; c) La parte medial estaba compuesta de tejido conectivo suelto, fibras de colágenos tipo I y tipo III, fibras elásticas y reticulares. En el presente estudio, la morfología de la AP en fetos y adultos se ajustó a la descripción anatómica estándar con variaciones en el origen de la parte medial observada en fetos. Además, los especímenes fetales mostraron una estructura de dos partes de la AP cuando la parte medial estaba ausente. Microscópicamente, los hallazgos sugieren que sólo las partes central y lateral pueden considerarse como AP, mientras que la parte medial puede denominarse FP.

The Anatomy and Morphology of the Superficial Peroneal Nerve.

Background Knowledge on the anatomical and morphological characteristics of the superficial peroneal nerve is amenable to further refinement. This cadaveric study aimed to further evaluate anatomical and morphological characteristics of the superficial peroneal nerve. Methods In this study, 10 lower limbs from five fresh cadavers were dissected. The anatomical characteristics of the superficial peroneal nerve were identified. Nerve segments were submitted for histological and morphometric analyses, and nerve thicknesses and number of fascicles were assessed. Results Regarding the superficial peroneal nerve's bifurcation, 80% of the terminal branches were distal to the point of emergence from the fascia. In 90% limbs, two sensory branches were observed immediately after the distal bifurcation of the superficial peroneal nerve. The mean distance from the fibular head to the superficial peroneal nerve's emergence from the fascia was 24.6 cm and mean nerve thickness at this point was 0.3 cm. The mean distance between the lateral malleolus and the main nerve trunk at the ankle was 4.68 cm. The mean distance from the motor branch of the peroneus brevis to the lateral malleolus was 29.3 cm. Morphometric analyses revealed an average five nerve bundles at the broadest nerve diameter (2.6 mm). Conclusion The anatomical and morphometrical characteristics of the superficial peroneal nerve indicate that it may be a safe and useful donor for autologous graft treatment of peripheral nerve injuries. Our morphological study shows a median of five fascicles, and that the thickest diameter of the nerve was 2.6 mm at the emergence from the deep to the superficial compartment.

In vivo relationship between pelvis motion and deep fascia displacement of the medial gastrocnemius: anatomical and functional implications.

Different authors have modelled myofascial tissue connectivity over a distance using cadaveric models, but in vivo models are scarce. The aim of this study was to evaluate the relationship between pelvic motion and deep fascia displacement in the medial gastrocnemius (MG). Deep fascia displacement of the MG was evaluated through automatic tracking with an ultrasound. Angular variation of the pelvis was determined by 2D kinematic analysis. The average maximum fascia displacement and pelvic motion were 1.501 ±â€…0.78 mm and 6.55 ±â€…2.47 °, respectively. The result of a simple linear regression between fascia displacement and pelvic motion for three task executions by 17 individuals was r = 0.791 (P < 0.001). Moreover, hamstring flexibility was related to a lower anterior tilt of the pelvis (r = 0.544, P < 0.024) and a lower deep fascia displacement of the MG (r = 0.449, P < 0.042). These results support the concept of myofascial tissue connectivity over a distance in an in vivo model, reinforce the functional concept of force transmission through synergistic muscle groups, and grant new perspectives for the role of fasciae in restricting movement in remote zones.

Structural study of endopelvic fascia in prostates of different weights. Anatomic study applied to radical prostatectomy.

PURPOSE: To evaluate the structure of the endopelvic fascia in prostates of different weights. METHODS: We studied 10 patients with BPH (prostates> 90 g); 10 patients with prostate adenocarcinoma (PAC) (prostates< 60 g) and five young male cadavers (control group). During the surgery a small sample of endopelvic fascia was obtained. We analyzed elastic fibers, collagen and smooth muscle. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using the one-way ANOVA with the Bonferroni test and a p<0.05 was considered statistically significant. RESULTS: The mean of the prostate weight was 122 g in BPH patients, 53.1g in PAC patients and 18.6g in control group. Quantitative analysis documented that there are no differences (p=0.19) in Vv of elastic fibers and in Vv of type III collagen (p=0.88) between the three groups. There was a significant difference (p=0<0.0001) in the quantification of SMC in patients with prostates > 90 g (mean=9.61%) when compared to patients with prostates < 60 g (mean=17.92%) and with the control group (mean=33.35%). CONCLUSION: There are differences in endopelvic fascia structure in prostates> 90 g, which can be an additional factor for pre-operatory evaluation of radical prostatectomy.

Structural study of endopelvic fascia in prostates of different weights. Anatomic study applied to radical prostatectomy

PURPOSE: To evaluate the structure of the endopelvic fascia in prostates of different weights.METHODS: We studied 10 patients with BPH (prostates>90g); 10 patients with prostate adenocarcinoma (PAC) (prostates 60g) and five young male cadavers (control group). During the surgery a small sample of endopelvic fascia was obtained. We analyzed elastic fibers, collagen and smooth muscle. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using the one-way ANOVA with the Bonferroni test and a p 0.05 was considered statistically significant.RESULTS:The mean of the prostate weight was 122 g in BPH patients, 53.1g in PAC patients and 18.6g in control group. Quantitative analysis documented that there are no differences (p=0.19) in Vv of elastic fibers and in Vv of type III collagen (p=0.88) between the three groups. There was a significant difference (p=0 0.0001) in the quantification of SMC in patients with prostates >90g (mean=9.61%) when compared to patients with prostates 60g (mean=17.92%) and with the control group (mean=33.35%).CONCLUSION:There are differences in endopelvic fascia structure in prostates>90g, which can be an additional factor for pre-operatory evaluation of radical prostatectomy.(AU)

Structural study of endopelvic fascia in prostates of different weights. Anatomic study applied to radical prostatectomy

PURPOSE: To evaluate the structure of the endopelvic fascia in prostates of different weights. METHODS: We studied 10 patients with BPH (prostates>90g); 10 patients with prostate adenocarcinoma (PAC) (prostates<60g) and five young male cadavers (control group). During the surgery a small sample of endopelvic fascia was obtained. We analyzed elastic fibers, collagen and smooth muscle. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using the one-way ANOVA with the Bonferroni test and a p<0.05 was considered statistically significant. RESULTS: The mean of the prostate weight was 122 g in BPH patients, 53.1g in PAC patients and 18.6g in control group. Quantitative analysis documented that there are no differences (p=0.19) in Vv of elastic fibers and in Vv of type III collagen (p=0.88) between the three groups. There was a significant difference (p=0<0.0001) in the quantification of SMC in patients with prostates >90g (mean=9.61%) when compared to patients with prostates <60g (mean=17.92%) and with the control group (mean=33.35%). CONCLUSION: There are differences in endopelvic fascia structure in prostates>90g, which can be an additional factor for pre-operatory evaluation of radical prostatectomy. .

Regeneration of periosteum by human bone marrow stromal cell sheets.

PURPOSE: The presence of a functional periosteum accelerates healing in bone defects by providing a source of progenitor cells that aid in repair. We hypothesized that bone marrow stromal cell (BMSC) sheets could be used to engineer functional periosteal tissues. MATERIALS AND METHODS: BMSCs were cultured to hyperconfluence and produced sufficient extracellular matrix to form robust tissue sheets. The sheets were wrapped around calcium phosphate pellets and implanted subcutaneously in mice for 8 weeks. Histologic comparisons were made between calcium phosphate samples with and without BMSC sheet wraps. Bone and periosteum formation were analyzed through tissue morphology and tissue-specific protein expression. RESULTS: Calcium phosphate pellets wrapped in BMSC sheets regenerated a bone-like tissue, but pellets lacking the cell sheet wrap did not. The bone-like tissue seen on the calcium phosphate scaffolds wrapped with the BMSC sheets was enclosed within a periosteum-like tissue characterized morphologically and through expression of periostin. CONCLUSIONS: These data indicate that cell sheet technology has potential for regenerating a functional periosteum-like tissue that could aid in future orthopedic therapy.

Petrus Camper: A history and overview of the clinical importance of Camper's fascia in surgical anatomy.

Petrus Camper's contributions to modern anatomical science include descriptions of the foot, upper limb, axilla, and inguinal region. His explanation of the etiology of inguinal hernias revolutionized the surgical practice of their repair. Camper's description of abdominal anatomy was an invaluable contribution to the field of abdominal surgery. Current research reviewed in this article shows the importance of understanding the layers of the abdominal wall, notably Camper's fascia, the closure of which has been found to be vital to proper wound healing and patient recovery. This article begins with a biography of Petrus Camper and his research in the inguinal region. It continues with anatomical and histological descriptions of Camper's fascia and finishes with its clinical correlates in surgical practice.

Anatomia aplicada do ramo temporal do nervo facial: estudo do risco potencial de lesão durante a cirurgia do rejuvenescimento facial / Applied anatomy of the branch of the facial nerve: study of the potential risk of injury during facial rejuvenation surgery

Introdução: O ramo temporal do nervo facial é um dos nervos mais comumente lesados, devido à pouca tela subcutânea que o protege a partir da sua saída da glândula parótida. Método: Vinte e cinco hemifaces de cadáveres foram dissecadas e analisadas as relações entre o ramo temporal e glândula parótida, arco zigomático, SMAS, artéria temporal superficial e músculo frontal. Resultados: Doze ramos temporais dissecados perderam a proteção da glândula parótida a uma distância de 1,7 cm anterior ao trago. O cruzamentodo arco zigomático por dois ramos temporais foi o mais frequente. A passagem pelo arco zigomático ocorreu entre 3,2 e 3,9 cm posteriores à borda lateral da órbita. O curso do ramo temporal junto às faces profundas do SMAS e da fáscia temporoparietal, e acima da lâmina superficial da fáscia temporal profunda foi constante. O ramo frontal da artéria temporal superficial foi superior e sua trajetória paralela ao ramo temporal em 92% das dissecções. Conclusão: O ramo temporal do nervo facial segue um plano constante ao longo da face profunda da fáscia temporoparietal e está muito superficial quando cruza o arco zigomático.

Background: The temporal branch of the facial nerve is one of the nerves more commonly injured due to the scarce subcutaneous tissue that protects it from its exit at the parotid gland. Method: Twenty five cadaveric hemifaces were dissected allowing a analysis of the temporal branch and: parotid gland, zygomatic arch, SMAS, superficial temporal artery and frontal muscle. Results: Twelve temporal branches exposed at a distance 1.7cm anterior to the trago. Two temporal branches crossing the zygomatic arc was the most frequent finding. This passage occurred between 3.2 to 3.9 cm posterior to the lateral border of the orbit. The trajectory of the temporal branch near the deep side of the SMAS and temporofacial fascia and above the superficial layer of the deep temporal fascia was constant. The frontal branch of the superficial temporal artery was superior and parallel to the nerve trajectory in 92% of the dissections Conclusion: The temporal branch follow a constant plane along the under surface of the temporoparietal fascia and is quite superficial as it cross the zygomatic arch.

Manejo del sulcus vocalis con injerto de fascia temporal / Sulcus vocalis management with fascia temporal graft

El sulcus vocalis es una lesión en la cual el epitelio del pliegue vocal tiende a invaginarse y adherirse al ligamento y/o músculo resultando en disfonía. Existen múltiples tratamientos descritos ninguno con resultados ideales. Este es un estudio descriptivo-prospectivo en el Hospital Militar Central en pacientes operados por sulcus vocalis tipos II y III e implantados con fascia autóloga entre junio de 2006 y diciembre de 2008. De 17 pacientes operados cumplieron los criterios de inclusión 11. Edad promedio 32 años. 9 presentaron mejoría del análisis acústico de la voz con una tendencia a la mejoría en todas las variables, particularmente en el Shimmer y la frecuencia fundamental. En la estroboscopia, 10 pacientes presentaron recuperación de la onda mucosa y en 5 del cierre glótico. Ningún paciente presentó reacciones adversas al procedimiento. Del presente estudio se puede considerar que el manejo del sulcus vocalis con injerto autólogo de fascia temporal es un procedimiento seguro que en la mayoría de los casos representa una mejoría subjetiva y objetiva de la calidad de voz. Es necesario aumentar el tamaño de la muestra para obtener resultados de mayor poder estadístico y definir los criterios de éxito.

Sulcus vocalis is an injury in which the epithelium of the vocal fold tends to invaginate and attach to the ligament and / or muscle resulting in dysphonia. There are multiple treatments as described but none has ideal results. This is a descriptive – prospective study carried out at the Hospital Militar Central in patients that had been operated on due to sulcus vocalis of the type II and III and who have been given implants with autologous fascia between June, 2006 and December, 2008. Out of 17 patients that were operated on, 11 complied with the inclusion criteria. The average age was 32 years old. 9 of them exhibited an improvement on the acoustic analysis of the voice with a tendency to improvement in all variables, especially in Shimmer and the basic frequency. 10 patients exhibited a recovery of the mucous wave in the stroboscope analysis and 5 in the glottal closure. Not one patient showed adverse reactions to the procedure. It can be considered from this study that managing sulcus vocalis with a temporalis fascia autologous graft is a safe procedure that represents a subjective and an objective improvement of the quality of the voice. It is necessary to increase the size of the simple in order to obtain results with a greater statistical power and be able to define the criteria for success.

Anatomy and biomechanics of the vertebral aponeurosis part of the posterior layer of the thoracolumbar fascia.

There is significant paucity in the literature regarding vertebral aponeurosis. We were able to find only a few descriptions of this specific fascia in the extant medical literature. To elucidate further the anatomy of this structure, forty adult human cadavers were dissected. Both quantitation and anatomical observations were made of the vertebral aponeurosis. The vertebral aponeurosis was identified in 100% of specimens. This fascia was identified as a thin fibrous layer consisting of longitudinal and transverse connective tissue fibers blended together deep to the latissimus dorsi muscle. It attached medially to the spinous processes of the of the thoracic vertebrae; laterally to the angles of ribs; inferiorly to the fascia covering the serratus posterior inferior muscle (superficial lamina of the posterior layer of thoracolumbar fascia); superiorly it ran deep to the serratus posterior superior and splenius capitis muscles to blend with the deep fascia of the neck. At the level of the serratus posterior inferior muscle, the vertebral aponeurosis fused to form a continuous layer descending toward the sacrotuberous ligament covering the erector spinae muscle. Morphometrically, the mean length of the vertebral aponeurosis was 38 cm and the mean width was 24 cm. The mean thickness was three mm. There was no significant difference between left and right sides, gender or age with regard to vertebral aponeurosis length, width, or thickness (P > 0.05). During manual tension of the vertebral aponeurosis, the tensile force necessary for failure had a mean of 38.7 N. In all specimens, the vertebral aponeurosis was capable of holding sutures placed through its substance. We hope that these data will be of use for descriptive purposes and may potentially add to our understanding of the biomechanics involved in movements of the back. As back pain is perhaps the most common reason patients visit their physicians, additional knowledge of this anatomical region is important.

Augmentation mammoplasty using the retrofascia approach.

In augmentation mammoplasty, the selection of placement for implants is based on the characteristics of each patient and the tissue conditions to ensure an optimal covering of these. The location must not be arbitrarily based on the preferences of the surgeon and the patient, but must ensure adequate tissue covering, thus minimizing shorts and long-term risks. Retromammary, retropectoral, and total and mixed retromuscular implants have been used. The retrofascia location of the mammary prosthesis reported in this article was described for the first time in the year 2000. This retrofascia technique refers to the collocation of the implant behind the fascia and in front of the pectoralis major muscle with the goal of achieving proper covering and good support of the implant. This is a nonrandomized prospective study investigated 110 cases of mammary hypoplasia and ptosis levels 1 and 2 from October 2001 to October 2002 with an average follow-up period of 1 year. Periareolar incisions were used in the retrofascia collocation of the mammary implant. The types of implants used included texturized silicone gel (85%) and smooth silicone gel (15%). As demonstrated by the findings, this surgical technique can achieve a good support to keep the prosthesis in an adequate position, maintaining the superior pole with a very natural projection. The incidence of complications was 7.6%, the most frequent of which was capsular contracture (3.53%).