Effects of Flammulinavelutipes Stem Base on Microflora and Volatile Fatty Acids In Caecum of Growing Layers under Heat Stress Condition

The present study was undertaken to investigate the effects of Flammulinavelutipes stem base (FVS) on growth performance, microbial flora and volatile fatty acids of growing layers under heat stress condition. A total of 72 ISA Brown hens were randomly divided into six treatments: thermoneutral temperature control group (CON), heat stress control group (HS), heat stress antibiotic group (ANT) as positive control and heat stress FVS groups (20, 40 or 60 g/kg FVS). The experimental period had a duration of 28 d (days 84-112). On day 98, daily gain average was significantly higher (p 0.05) in the FVS groups than in the HS group. The number of bands in the FVS groups were higher (p 0.05) than in the HS group on day 98. The microbial similarity between the 60 g/kg FVS group and the HS group were the lowest on day 98. FVS groups specific bacteria were mainly Coprococcus comes, [Clostridium] papyrosolvens, Butyricicoccuspullicaecorumon day 98. Whereas on day 112, the FVS groups specific bacteria were mainly Parabacteroides distasonis, Coprobacterfastidiosus, Elusimicrobiumminutum. The content of acetic acid and butyric acid were higher (p 0.05) in 20 g/kg FVS group than in the CON group on day 112. In conclusion, FVS can lighten the adverse effect of heat stress by increasing the diversity of intestinal flora in growing layers.

Effects of Flammulinavelutipes Stem Base on Microflora and Volatile Fatty Acids In Caecum of Growing Layers under Heat Stress Condition

The present study was undertaken to investigate the effects of Flammulinavelutipes stem base (FVS) on growth performance, microbial flora and volatile fatty acids of growing layers under heat stress condition. A total of 72 ISA Brown hens were randomly divided into six treatments: thermoneutral temperature control group (CON), heat stress control group (HS), heat stress antibiotic group (ANT) as positive control and heat stress FVS groups (20, 40 or 60 g/kg FVS). The experimental period had a duration of 28 d (days 84-112). On day 98, daily gain average was significantly higher (p 0.05) in the FVS groups than in the HS group. The number of bands in the FVS groups were higher (p 0.05) than in the HS group on day 98. The microbial similarity between the 60 g/kg FVS group and the HS group were the lowest on day 98. FVS groups specific bacteria were mainly Coprococcus comes, [Clostridium] papyrosolvens, Butyricicoccuspullicaecorumon day 98. Whereas on day 112, the FVS groups specific bacteria were mainly Parabacteroides distasonis, Coprobacterfastidiosus, Elusimicrobiumminutum. The content of acetic acid and butyric acid were higher (p 0.05) in 20 g/kg FVS group than in the CON group on day 112. In conclusion, FVS can lighten the adverse effect of heat stress by increasing the diversity of intestinal flora in growing layers.(AU)

Organic egg production, egg quality, calcium utilization, and digestibility in laying hens fed with mushroom (Flammulina velutipes) stem waste

The possibility of using mushroom (Flammulina velutipes) stem waste (FVW) in layer diets was explored in this study. A total of 180, 40-week-old ISA Brown laying hens were randomly assigned to 5 groups, with 6 replications of 6 birds for each group. Layers were fed a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW. No significant differences (p>0.05) were observed on hen daily egg production, egg weight, egg mass, unmarketable eggs, feed intake, feed conversion ratio, and live weight among experimental groups. Haugh unit was found higher (p0.05) by feeding FVW in this study. Yolk color was found higher (p<0.05) in FVW fed groups than in control and antibiotic groups. The shell color was higher (p<0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups. Calcium retention and calcium in egg shell deposition were higher (p<0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups respectively. Apparent nutrient retention for dry matter, crude protein, crude fat, and organic matter were higher (p<0.05) in FVW fed groups than in control and antibiotic groups. FVW at 6% level in layer ration can be used for organic eggs production which would be safe and sound for human consumption.

Organic egg production, egg quality, calcium utilization, and digestibility in laying hens fed with mushroom (Flammulina velutipes) stem waste

The possibility of using mushroom (Flammulina velutipes) stem waste (FVW) in layer diets was explored in this study. A total of 180, 40-week-old ISA Brown laying hens were randomly assigned to 5 groups, with 6 replications of 6 birds for each group. Layers were fed a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW. No significant differences (p>0.05) were observed on hen daily egg production, egg weight, egg mass, unmarketable eggs, feed intake, feed conversion ratio, and live weight among experimental groups. Haugh unit was found higher (p<0.05) in FVW fed groups than in antibiotic fed groups. Shape index, shell weight, shell thickness, yolk weight, yolk index, yolk weight and albumen weight were not affected (p>0.05) by feeding FVW in this study. Yolk color was found higher (p<0.05) in FVW fed groups than in control and antibiotic groups. The shell color was higher (p<0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups. Calcium retention and calcium in egg shell deposition were higher (p<0.05) both in 4% FVW and 6% FVW fed groups than in control and antibiotic groups respectively. Apparent nutrient retention for dry matter, crude protein, crude fat, and organic matter were higher (p<0.05) in FVW fed groups than in control and antibiotic groups. FVW at 6% level in layer ration can be used for organic eggs production which would be safe and sound for human consumption.(AU)

Flammulina velutipes: An option for "alperujo" use.

BACKGROUND: Two-phase olive-mill wastes (or "alperujo") exhibit highly phytotoxic properties, mainly due to phenols. A valuable option for alperujo is its agricultural use, provided that no phytotoxic effects occur. AIMS: The present investigation was aimed at evaluating the efficacy of two strains of the lignin-degrading fungus Flammulina velutipes to colonize alperujo in order to produce edible mushrooms and to achieve its detoxification. METHODS: Some important cultural characters related to mushroom production (earliness, biological efficiency and quality of basidiomes) were estimated. The production of lignocellulolytic enzymes, phenol removal and detoxification of the substrate was evaluated. RESULTS: High biological efficiencies (70.8%) were obtained at 12°C with F. velutipes strain BAFC 670/06 in a substrate containing poplar wood shavings and 90% of alperujo. The nature of the substrate did not seem to exert an important influence on pileus and stem morphology; nevertheless shortest stems were observed at higher temperatures. Endo-ß-1,4-glucanase, endo-ß-1,4-xylanase, laccase and Mn-peroxidase activities were detected in the extracts recovered from the solid-state cultures. Both F. velutipes strains were effective in removing the phenolic compounds. The initial concentration in the substrate with 90% alperujo was reduced in the case of F. velutipes BAFC 1763 by 84.31%, and 40.15% by F. velutipes BAFC 670/06. Germinability experiments on Raphanus sativus, showed that alperujo phytotoxicity was significantly reduced by F. velutipes cultures. CONCLUSIONS: The experimented changes by the spent mushroom substrate resulting from F. velutipes cultivation with high amount of alperujo would allow its reuse for agricultural purposes.

Characterization of ß-glucosidase produced by the white rot fungus Flammulina velutipes.

ß-Glucosidase production by the white rot fungus Flammulina velutipes CFK 3111 was evaluated using different carbon and nitrogen sources under submerged fermentation. Maximal extracellular enzyme production was 1.6 U/ml, corresponding to a culture grown in sucrose 40 g/l and asparagine 10 g/l. High production yield was also obtained with glucose 10 g/l and asparagine 4 g/l medium (0.5 U/ml). Parameters affecting the enzyme activity were studied using p-nitrophenyl-ß-D-glucopyranoside as the substrate. Optimal activity was found at 50°C and pHs 5.0 to 6.0. Under these conditions, ß-glucosidase retained 25% of its initial activity after 12 h of incubation and exhibited a half-life of 5 h. The addition of MgCl2, urea, and ethanol enhanced the ß-glucosidase activity up to 47%, whereas FeCl2, CuSO4, Cd(NO3)2, and cetyltrimethylammonium bromide inflicted a strong inhibitory effect. Glucose and cellobiose also showed an inhibitory effect on the ß-glucosidase activity in a concentration-dependent manner. The enzyme had an estimated molecular mass of 75 kDa. To the best of our knowledge, F. velutipes CFK 3111 ß-glucosidase production is amongst the highest reported to date, in a basidiomycetous fungus.

Use of P450 cytochrome inhibitors in studies of enokipodin biosynthesis

Enokipodins A, B, C, and D are antimicrobial sesquiterpenes isolated from the mycelial culture medium of Flammulina velutipes, an edible mushroom. The presence of a quaternary carbon stereocenter on the cyclopentane ring makes enokipodins A-D attractive synthetic targets. In this study, nine different cytochrome P450 inhibitors were used to trap the biosynthetic intermediates of highly oxygenated cuparene-type sesquiterpenes of F. velutipes. Of these, 1-aminobenzotriazole produced three less-highly oxygenated biosynthetic intermediates of enokipodins A-D; these were identified as (S)-(-)-cuparene-1,4-quinone and epimers at C-3 of 6-hydroxy-6-methyl-3-(1,2,2-trimethylcyclopentyl)-2-cyclohexen-1-one. One of the epimers was found to be a new compound.

Use of P450 cytochrome inhibitors in studies of enokipodin biosynthesis.

Enokipodins A, B, C, and D are antimicrobial sesquiterpenes isolated from the mycelial culture medium of Flammulina velutipes, an edible mushroom. The presence of a quaternary carbon stereocenter on the cyclopentane ring makes enokipodins A-D attractive synthetic targets. In this study, nine different cytochrome P450 inhibitors were used to trap the biosynthetic intermediates of highly oxygenated cuparene-type sesquiterpenes of F. velutipes. Of these, 1-aminobenzotriazole produced three less-highly oxygenated biosynthetic intermediates of enokipodins A-D; these were identified as (S)-(-)-cuparene-1,4-quinone and epimers at C-3 of 6-hydroxy-6-methyl-3-(1,2,2-trimethylcyclopentyl)-2-cyclohexen-1-one. One of the epimers was found to be a new compound.

Expression of an oxalate decarboxylase impairs the necrotic effect induced by Nep1-like protein (NLP) of Moniliophthora perniciosa in transgenic tobacco.

Oxalic acid (OA) and Nep1-like proteins (NLP) are recognized as elicitors of programmed cell death (PCD) in plants, which is crucial for the pathogenic success of necrotrophic plant pathogens and involves reactive oxygen species (ROS). To determine the importance of oxalate as a source of ROS for OA- and NLP-induced cell death, a full-length cDNA coding for an oxalate decarboxylase (FvOXDC) from the basidiomycete Flammulina velutipes, which converts OA into CO(2) and formate, was overexpressed in tobacco plants. The transgenic plants contained less OA and more formic acid compared with the control plants and showed enhanced resistance to cell death induced by exogenous OA and MpNEP2, an NLP of the hemibiotrophic fungus Moniliophthora perniciosa. This resistance was correlated with the inhibition of ROS formation in the transgenic plants inoculated with OA, MpNEP2, or a combination of both PCD elicitors. Taken together, these results have established a pivotal function for oxalate as a source of ROS required for the PCD-inducing activity of OA and NLP. The results also indicate that FvOXDC represents a potentially novel source of resistance against OA- and NLP-producing pathogens such as M. perniciosa, the causal agent of witches' broom disease of cacao (Theobroma cacao L.).