ABSTRACT
Biobased furans have emerged as chemical building blocks for the development of materials because of their diverse scaffolds and as they can be directly prepared from sugars. However, selective, efficient, and cost-effective scalable conversion of biobased furans remains elusive. Here, we report a robust transaminase (TA) from Shimia marina (SMTA) that enables the scalable amination of biobased furanaldehydes with high activity and broad substrate specificity. Crystallographic and mutagenesis analyses provide mechanistic insights and a structural basis for understanding SMTA, which enables a higher substrate conversion. The enzymatic cascade process established in this study allows one-pot synthesis of 2,5-bis(aminomethyl)furan (BAMF) and 5-(aminomethyl)furan-2-carboxylic acid from 5-hydroxymethylfurfural. The biosynthesis of various furfurylamines, including a one-pot cascade reaction for BAMF generation using whole cells, demonstrates their practical application in the pharmaceutical and polymer industries.
Subject(s)
Biocatalysis , Furans , Transaminases , Furans/chemistry , Furans/metabolism , Transaminases/metabolism , Transaminases/genetics , Transaminases/chemistry , Substrate Specificity , Furaldehyde/analogs & derivatives , Furaldehyde/metabolism , Furaldehyde/chemistry , Amination , Amines/chemistry , Amines/metabolism , Crystallography, X-RayABSTRACT
A facile biphasic system composed of choline chloride (ChCl)-based deep eutectic solvent (DES) and methyl isobutyl ketone (MIBK) was developed to realize the furfural production, lignin separation and preparation of fermentable glucose from Eucalyptus in one-pot. Results showed that the ChCl/1,2-propanediol/MIBK system owned the best property to convert hemicelluloses into furfural. Under the optimal conditions (MRChCl:1,2-propanediol = 1:2, raw materials:DES:MIBK ratio = 1:4:8 g/g/mL, 0.075 mol/L AlCl3·6H2O, 140 °C, and 90 min), the furfural yield and glucose yield reached 65.0 and 92.2 %, respectively. Meanwhile, the lignin with low molecular weight (1250-1930 g/mol), low polydispersity (DM = 1.25-1.53) and high purity (only 0.08-2.59 % carbohydrate content) was regenerated from the biphasic system. With the increase of pretreatment temperature, the ß-O-4, ß-ß and ß-5 linkages in the regenerated lignin were gradually broken, and the content of phenolic hydroxyl groups increased, but the content of aliphatic hydroxyl groups decreased. This research provides a new strategy for the comprehensive utilization of lignocellulose in biorefinery process.
Subject(s)
Cellulose , Eucalyptus , Furaldehyde , Lignin , Eucalyptus/chemistry , Eucalyptus/metabolism , Lignin/chemistry , Furaldehyde/chemistry , Furaldehyde/analogs & derivatives , Furaldehyde/metabolism , Cellulose/chemistry , Methyl n-Butyl Ketone/chemistry , Methyl n-Butyl Ketone/metabolism , Choline/chemistry , Propylene Glycol/chemistry , Glucose/metabolism , Glucose/chemistry , Fermentation , Solvents/chemistryABSTRACT
The baking process has the potential to generate health-risk compounds, including products from lipid oxidation and Maillard reaction. Pre- and post-digestion levels of hydroxymethylfurfural (HMF), malondialdehyde (MDA), glyoxal (GO), and methylglyoxal (MGO) were studied in cakes formulated with hazelnut and sunflower oil, along with their oleogels as margarine substitutes. The concentration of HMF in oil and oleogel-formulated cakes increased after digestion compared to cakes formulated with margarine. The MDA values were between 82 and 120 µg/100 g in oil and oleogel formulated cakes before digestion and a decrease was observed after digestion. The substitution of margarine with oil and oleogels resulted in the production of high amounts of GO and MGO in cakes. However, the highest bioaccessibility as 318.2% was found in cakes formulated by margarine for GO. Oleogels may not pose a potential health benefit compared to margarines due to the formation of HMF, MDA, GO, and MGO.
Subject(s)
Digestion , Furaldehyde , Malondialdehyde , Organic Chemicals , Furaldehyde/analogs & derivatives , Furaldehyde/chemistry , Furaldehyde/analysis , Malondialdehyde/metabolism , Malondialdehyde/chemistry , Organic Chemicals/chemistry , Humans , Gastrointestinal Tract/metabolism , Corylus/chemistry , Glycation End Products, Advanced/chemistry , Glycation End Products, Advanced/metabolism , Models, Biological , Sunflower Oil/chemistry , Margarine/analysisABSTRACT
Pasteurisation and spray drying are critical steps to ensure the safety and shelf-life of formulae, but these treatments also induce formation of some potentially harmful Maillard reaction products. In this study, the occurrence of potentially harmful Maillard reaction products and proximate compositions in different commercial formulae were analysed. Our results showed that infant formulae had significantly higher concentrations of furosine, Nε-(carboxymethyl)lysine (CML) and Nε-(carboxyethyl)lysine (CEL) than follow-on/toddler formula. Specialty formulae had higher concentrations of glyoxal and CML than other types of formulae. Correlation analysis indicated that concentrations of 5-hydroxymethylfurfural, 3-deoxyglucosone, CML and CEL were closely related to fat contents. These results provided insight into concentrations of potentially harmful Maillard reaction products in different types of formulae and provide a theoretical basis for further optimisation of processing.
Subject(s)
Infant Formula , Lysine , Maillard Reaction , Infant Formula/chemistry , Infant Formula/analysis , Lysine/chemistry , Lysine/analogs & derivatives , Lysine/analysis , Humans , Furaldehyde/analogs & derivatives , Furaldehyde/analysis , Furaldehyde/chemistry , Glyoxal/chemistry , Glyoxal/analysis , Infant , Deoxyglucose/analogs & derivatives , Deoxyglucose/chemistry , Deoxyglucose/analysisABSTRACT
Fabrication of sustainable bio-based malleable thermosets (BMTs) with excellent mechanical properties and reprocessing ability for applications in electronic devices has attracted more and more attention but remains significant challenges. Herein, the BMTs with excellent mechanical robustness and reprocessing ability were fabricated via integrating with radical polymerization and Schiff-base chemistry, and employed as the flexible substrate to prepare the capacitive sensor. To prepare the BMTs, an elastic bio-copolymer derived from plant oil and 5-hydroxymethylfurfural was first synthesized, and then used to fabricate the dynamic crosslinked BMTs through Schiff-base chemistry with the amino-modified cellulose and polyether amine. The synergistic effect of rigid cellulose backbone and the construction of dynamic covalent crosslinking network not only achieved high tensile strength (8.61 MPa) and toughness (3.77 MJ/m3) but also endowed the BMTs with excellent reprocessing ability with high mechanical toughness recovery efficiency of 104.8 %. More importantly, the BMTs were used as substrates to fabricate the capacitive sensor through the CO2-laser irradiation technique. The resultant capacitive sensor displayed excellent and sensitive humidity sensing performance, which allowed it to be successfully applied in human health monitoring. This work paved a promising way for the preparation of mechanical robustness malleable bio-thermosets for electronic devices.
Subject(s)
Cellulose , Furaldehyde , Plant Oils , Cellulose/chemistry , Furaldehyde/chemistry , Furaldehyde/analogs & derivatives , Plant Oils/chemistry , Electric Capacitance , Temperature , Tensile Strength , HumansABSTRACT
Pentanediols are substances with significant market potential as the key monomers for advanced polymeric materials. In this study, we successfully achieved directly hydrogenolysis of biomass-based furfural to 1,5-pentanediol with a remarkable yield of 53.4 % using Cu-modified cobalt supported on cerium dioxide catalysts. Through comprehensive characterization techniques, including H2-TPR, NH3-TPD, XPS, EPR and Raman analysis, the study revealed that the introduction of Cu altered the dispersion of Co species, attenuated the interaction between Co species and cerium dioxide, enhanced its reduction extent, and fostered the formation of plentiful cobalt oxide species and oxygen vacancies on the catalyst's surface. The cooperative influence of Cu and Co heightened the selectivity of the hydrogenolysis reaction. This work provides a novel strategy for the development of greener and more efficient catalytic processes based on non-precious metals that for the selective conversion of biomass-derived furfural to high-value pentanediols.
Subject(s)
Cobalt , Copper , Furaldehyde , Cobalt/chemistry , Catalysis , Copper/chemistry , Furaldehyde/chemistry , Cerium/chemistry , Glycols/chemistry , BiomassABSTRACT
Catalytic transfer hydrogenation (CTH), that employs protic solvents as hydrogen sources to alleviate the use of molecular hydrogen H2, has gained great attention. This work, reports multifunctional, metallic Cu nanoparticles supported ZIF-8 material for CTH of furfural to a highly valued fuel additive, 2-methylfuran (2-MF) using 2-propanol. Of all as-synthesized xCu(yM)/ZIF-8 catalysts with varied NaBH4 concentration (yM) and Cu loading (x), 11Cu(1.5 M)/ZIF-8 exhibited higher catalytic activity with > 99 % FAL conversion and 93.9 % 2-MF selectivity. This is ascribed to its high specific surface area, and existence of optimum amount of Lewis acid-base sites along with Cu0 species, which are responsible for hydrogenation of furfural to furfuryl alcohol and subsequent hydrogenolysis to produce 2-MF. The present work reports a highly efficient and stable, metal-MOF hybrid material for CTH of FAL to 2-MF, which is one among the best reports available in literature, therewith suggests a promising approach for bio-oil upgradation.
Subject(s)
Copper , Furaldehyde , Furans , Metal Nanoparticles , Zeolites , Furans/chemistry , Catalysis , Hydrogenation , Copper/chemistry , Furaldehyde/chemistry , Furaldehyde/analogs & derivatives , Zeolites/chemistry , Metal Nanoparticles/chemistry , Hydrogen/chemistryABSTRACT
Lactose hydrolysed concentrated milk was prepared using ß-galactosidase enzyme (4.76U/mL) with a reaction period of 12 h at 4 °C. Addition of polysaccharides (5 % maltodextrin/ß-cyclodextrin) to concentrated milk either before or after lactose hydrolysis did not result in significant differences (p > 0.05) in degree of hydrolysis (% DH) of lactose and residual lactose content (%). Three different inlet temperatures (165 °C, 175 °C and 185 °C) were used for the preparation of powders which were later characterised based on physico-chemical and maillard browning characteristics. Moisture content, solubility and available lysine content of the powders decreased significantly, whereas, browning parameters i.e., browning index, 5-hydroxymethylfurfural, furosine content increased significantly (p < 0.05) with an increase in inlet air temperature. The powder was finally prepared with 5 % polysaccharide and an inlet air temperature of 185 °C which reduced maillard browning. Protein-polysaccharide interactions were identified using Fourier Transform infrared spectroscopy, fluorescence spectroscopy and determination of free amino groups in the powder samples. Maltodextrin and ß-cyclodextrin containing powder samples exhibited lower free amino groups and higher degree of graft value as compared to control sample which indicated protein-polysaccharide interactions. Results obtained from Fourier Transform infrared spectroscopy also confirmed strong protein-polysaccharide interactions, moreover a significant decrease in fluorescence intensity was also observed in the powder samples. These interactions between the proteins and polysaccharides reduced the maillard browning in powders.
Subject(s)
Furaldehyde , Lactose , Maillard Reaction , Milk , Polysaccharides , Powders , Lactose/chemistry , Polysaccharides/chemistry , Milk/chemistry , Animals , Spectroscopy, Fourier Transform Infrared , Furaldehyde/analogs & derivatives , Furaldehyde/chemistry , beta-Galactosidase/metabolism , beta-Cyclodextrins/chemistry , Hydrolysis , Spray Drying , Temperature , Lysine/chemistry , Lysine/analogs & derivatives , Solubility , Spectrometry, Fluorescence , Milk Proteins/chemistry , Food Handling/methodsABSTRACT
Recently, there has been a growing interest in using sustainable energy to decrease lignin monomers to generate high-value-added products. Here, we present a protocol for electrocatalytic hydrogenation of 5-hydroxymethylfurfural. We describe steps for catalyst preparation, performing electrocatalytic experiments, high-performance liquid chromatography analysis, and in situ infrared reflection-absorption spectroscopy testing. The synthesized catalyst used in this reaction exhibits enhanced selectivity and Faradaic efficiency in NaClO4 solution. For complete details on the use and execution of this protocol, please refer to Zhang et al.1.
Subject(s)
Furaldehyde , Furaldehyde/analogs & derivatives , Furaldehyde/chemistry , Catalysis , Hydrogenation , Chromatography, High Pressure Liquid/methods , Electrochemical Techniques/methodsABSTRACT
The 5-nitro-2-furaldehyde (5-NF) is an aldehyde aromatic organic compound that has been envisaged as an alternative marker for detecting nitrofurazone treatment abuse and to avoid the false positive results induced by the semicarbazide. Analyzing 5-NF presents challenges, and its derivatization reaction with hydrazine reagents is required to enhance the capability of its detection and its identification. This study aims at developping an analytical method for 5-NF determination in trout muscle samples based on chemical derivatization prior to analysis by liquid chromatography-tandem mass spectrometry. Four commercially available hydrazine reagents, namely: N,N-Dimethylhydrazine (DMH), 4-Hydrazinobenzoic acid (HBA), 2,4-Dichlorophenylhydrazine (2,4-DCPH) and 2,6-Dichlorophenylhydrazine (2,6-DCPH) were proposed for the first time as derivatizing reagents in the analysis of 5-NF. The derivatization reaction was simultaneously performed along with the extraction method in acidic condition using ultrasonic assistance and followed by liquid extraction using acetonitrile. The efficiency of the chemical reaction with 5-NF was examined and the reaction conditions including the concentration of hydrochloric acid, pH, temperature, reaction time and the concentration of the derivatizing reagents were optimized. Experiments with fortified samples demonstrated that 2,4-DCPH derivatizing reagent at 20 mM for 20 min of ultrasonic treatment under acidic condition (pH 4) gave an effective sample derivatization method for 5-NF analysis. Under the optimized conditions, the calibration curves were linear from 0.25 to 2 µg kg-1 with coefficient of determination >0.99. The recoveries ranged from 89 % to 116 % and precision was less than 13 %. The limit of detection and quantification were 0.1 and 0.2 µg kg-1, respectively.
Subject(s)
Muscles , Tandem Mass Spectrometry , Trout , Tandem Mass Spectrometry/methods , Animals , Chromatography, Liquid/methods , Muscles/chemistry , Furaldehyde/analogs & derivatives , Furaldehyde/analysis , Furaldehyde/chemistry , Limit of Detection , Indicators and Reagents/chemistry , Hydrazines/chemistryABSTRACT
Gentianae Radix, an herbal medicine, has been used as a gastrointestinal drug in Japan. In the Japanese Pharmacopoeia 18th Revision, the sublimation test is specified as an identification test for Gentianae Radix. The compound obtained in this sublimation test was believed to be gentisin, a xanthone family compound. However, the compound we identified using liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and 1H- and 13C-NMR was 5-(hydroxymethyl)furfural (5-HMF). The same compound was found to be a sublimate of Gentianae Scabrae Radix and Gentianae Macrophyllae Radix, belonging to the same genus as Gentianae Radix. These results indicate the necessity to revise the identification test for Gentianae Radix to a more unique method.
Subject(s)
Furaldehyde , Gentiana , Furaldehyde/analogs & derivatives , Furaldehyde/chemistry , Gentiana/chemistry , Japan , Mass Spectrometry , Plant Roots/chemistry , Pharmacopoeias as Topic , Magnetic Resonance Spectroscopy , Chromatography, Liquid , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal/chemistry , East Asian PeopleABSTRACT
Nb-based catalysts supported on porous silica with different textural properties have been synthesized, characterized, and tested in the one-pot reaction of furfural to obtain valuable chemicals. The catalytic results reveal that the presence of fluoride in the synthesis, which limits the growing of the porous silica, limits diffusional problems of the porous silica, obtaining higher conversion values at shorter reaction times. On the other hand, the incorporation of NbOx species in the porous silica provides Lewis acid sites and a small proportion of Brönsted acid sites, in such a way that the main products are alkyl furfuryl ethers, which can be used as fuel additives.
Subject(s)
Furaldehyde , Niobium , Furaldehyde/chemistry , Hydrogenation , Silicon Dioxide/chemistry , CatalysisABSTRACT
Recently, catalytic valorization of biomass-derived furans has received growing interest. 5-Aminomethyl-2-furancarboxylic acid (AMFC), a furan amino acid, holds great promise in the aeras of polymer and pharmaceutical, but its synthesis remains limited. In this work, we report a chemobiocatalytic route toward AMFC by combining laccase-TEMPO system and recombinant Escherichia coli (named E.â coli_TAF) harboring ω-transaminase (TA), L-alanine dehydrogenase (L-AlaDH) and formate dehydrogenase (FDH), starting from 5-hydroxymethylfurfural (HMF). In the cascade, HMF is oxidized into 5-formyl-2-furancarboxylic acid (FFCA) by laccase-TEMPO system, and then the resulting intermediate is converted into AMFC by E.â coli_TAF via transamination with cheap ammonium formate instead of costly organic amine donors, theoretically generating H2O and CO2 as by-products. The tandem process was run in a one-pot twostep manner, affording AMFC with approximately 81 % yield, together with 10 % 2,5-furandicarboxylic acid (FDCA) as by-product. In addition, the scale-up production of AMFC was demonstrated, with 0.41â g/L h productivity and 8.6â g/L titer. This work may pave the way for green manufacturing of the furan-containing amino acid.
Subject(s)
Escherichia coli , Furaldehyde/analogs & derivatives , Laccase , Escherichia coli/metabolism , Laccase/chemistry , Amino Acids , Furans/chemistry , Furaldehyde/chemistry , Furaldehyde/metabolism , Dicarboxylic Acids/chemistryABSTRACT
The present study aimed to elucidate the pathway of pigment formation and identify the source of antioxidant activity during sugar smoking. Building upon previous research, this investigation replicated the sucrose cleavage process involved in sugar-smoking through model reactions to obtain distinct model reaction products. The products were analyzed using various techniques such as ultraviolet-visible spectrometry, Fourier-transform infrared spectroscopy, high-performance liquid chromatography, and high-performance liquid chromatography-tandem mass spectrometry. The findings revealed that the pyrolysis of sucrose at 330 °C yielded glucose and fructose, with fructose pyrolysis producing significantly more 5-HMF than glucose. Moreover, the antioxidant capacity of 5-HMF was found to make a substantial contribution. The primary source of 5-HMF was identified as fructose resulting from the cleavage of sucrose at 330 °C, while the primary pathway for the formation of the sugar-smoking pigment 5-GGMF was attributed to the intermolecular dehydration of 5-HMF and glucose at 150 °C.
Subject(s)
Antioxidants , Sugars , Carbohydrates/chemistry , Glucose/chemistry , Sucrose/chemistry , Fructose/chemistry , Smoking , Furaldehyde/chemistryABSTRACT
A systematic kinetic study was conducted in subcritical water medium in the temperature range from 150 to 200 °C for pure glucose, xylose, proline and aspartic acid as well as binary mixtures of sugars + amino acids to understand the reaction kinetics and interactions among biomass components and to discern the influence of Maillard reaction (MR) on the overall reaction kinetics. The main degradation products identified for glucose and xylose were the respective dehydration products, hydroxymethyl furfural and furfural, yielding an increasing solid residue with temperature (15.9 wt% at 200 °C) with an augmented heating value. The degradation of sugars and amino acids in binary systems was faster compared to pure compounds due to MR and the production of dehydration products was delayed when considering total sugar conversion. Higher relative reactivity in MR was observed for xylose over glucose showing also higher antioxidant activity.
Subject(s)
Amino Acids , Xylose , Humans , Xylose/chemistry , Glucose/chemistry , Sugars , Aspartic Acid/chemistry , Proline , Water/chemistry , Furaldehyde/chemistry , Maillard Reaction , Dehydration , KineticsABSTRACT
The purpose of this study is to design and investigate two coupling processes for acid-catalyzed hydrolysis of corncob, achieving the simultaneous preparation of biomass-based furfural and levulinic acid (LA). Meanwhile, high concentration and yield of LA were obtained through a situ feeding strategy of pretreated furfural residue with high solids loading (20%, w/v). In Scenario A, 2-methyltetrahydrofuran was selected as the solvent for the LA extraction process compared with the neutralization process in Scenario B. Techno-economic assessment results show that Scenario A is technically feasible and cost-competitive, with an internal rate of return of 21.92%, a net present value of 121 million US dollars, a carbon efficiency of 72%, an environmental factor of 4.38, and a process mass intensity of 32.19. This study will provide new insights for fully utilizing lignocellulosic biomass to prepare renewable energy resources, comprehensively evaluating the economic feasibility, and promoting green and low-carbon development.
Subject(s)
Furaldehyde , Zea mays , Furaldehyde/chemistry , Zea mays/chemistry , Biomass , Levulinic Acids , CarbonABSTRACT
Modern biocatalysis requires fast, sensitive, and efficient high-throughput screening methods to screen enzyme libraries in order to seek out novel biocatalysts or enhanced variants for the production of chemicals. For instance, the synthesis of bio-based furan compounds like 2,5-diformylfuran (DFF) from 5-hydroxymethylfurfural (HMF) via aerobic oxidation is a crucial process in industrial chemistry. Laccases, known for their mild operating conditions, independence from cofactors, and versatility with various substrates, thanks to the use of chemical mediators, are appealing candidates for catalyzing HMF oxidation. Herein, Schiff-based polymers based on the coupling of DFF and 1,4-phenylenediamine (PPD) have been used in the set-up of a novel colorimetric assay for detecting the presence of DFF in different reaction mixtures. This method may be employed for the fast screening of enzymes (Z' values ranging from 0.68 to 0.72). The sensitivity of the method has been proved, and detection (8.4 µM) and quantification (25.5 µM) limits have been calculated. Notably, the assay displayed selectivity for DFF and enabled the measurement of kinetics in DFF production from HMF using three distinct laccase-mediator systems.
Subject(s)
Furaldehyde , Laccase , Laccase/metabolism , Furaldehyde/chemistry , Oxidation-ReductionABSTRACT
The research on the efficient use of biomass to produce chemical products has received extensive attention. In this work, a novel heterogeneous biocarbon-based heterogeneous catalyst AT-Sn-YB was prepared using yellow bamboo (YB) as a carrier, and its physical properties were proved to be good by various characterization and stability experiments. In the γ-valerolactone/water (3:1, v/v) medium containing 100 mM CuCl2, the use of AT-Sn-YB (3.6 wt%) under 170 °C for 20 min was applied to catalyze YB into furfural (80.3% yield), accompanied with 2.8 g/L xylooligosaccharides. The YB solid residue obtained from treatment was efficiently saccharified to reducing sugars (17.2 g/L). Accordingly, comprehensive understanding of efficiently co-producing xylooligosaccharides, furfural and reducing sugars from YB was demonstrated via the pretreatment with biochar-based catalyst. This study innovatively used a new type of solid acid to complete the efficient co-production of chemical products, and realized the value-added utilization of yellow bamboo.
Subject(s)
Furaldehyde , Sugars , Furaldehyde/chemistry , CatalysisABSTRACT
In this study, the high-loaded copper-containing catalysts modified with Fe and Al were successfully applied for the hydroconversion of furfural to furfuryl alcohol (FA) or 2-methylfuran (2-MF) in a batch reactor. The synthesized catalysts were studied using a set of characterization techniques to find the correlation between their activity and physicochemical properties. Fine Cu-containing particles distributed in an amorphous SiO2 matrix, which has a high surface area, provide the conversion of furfural to FA or 2-MF under exposure to high pressure of hydrogen. The modification of the mono-copper catalyst with Fe and Al increases its activity and selectivity in the target process. The reaction temperature strongly affects the selectivity of the formed products. At a H2 pressure of 5.0 MPa, the highest selectivity toward FA (98%) and 2-MF (76%) was achieved in the case of 35Cu13Fe1Al-SiO2 at the temperature of 100 °C and 250 °C, respectively.
Subject(s)
Furaldehyde , Silicon Dioxide , Furaldehyde/chemistry , Silicon Dioxide/chemistry , Hydrogen/chemistry , Catalysis , TemperatureABSTRACT
The coexistence of anthocyanin with the sugar degradation product 5-hydroxymethylfurfural (5-HMF) is inevitable during the processing and storage of anthocyanin-rich juices. It was determined from our study that lower concentrations of 5-HMF have little effect on the stability of Cyanidin-3-O-glucoside (C3G), and even cause a slight increase for a short period of time. As the concentration of 5-HMF increased, the retention of C3G decreased and the color of the solution changed from orange-red to purple-red. The reaction sites of 5-HMF and C3G in its hemiketal form were predicted by quantum chemical calculations in order to investigate the pathways of action of the two. The degradation mechanism of 5-HMF on anthocyanin was verified by Ultraviolet and Visible spectrophotometer and Ultra performance liquid chromatography-mass spectrometry. Therefore, this article provides further theoretical support for the study of the effect of furfural compounds, which are sugar degradation products, on the stability of anthocyanins.