ABSTRACT
l-amino acid oxidases (LAAOs) are flavoenzymes that catalyze the oxidative deamination of l-amino acids to the corresponding α-keto acids, ammonia, and hydrogen peroxide. Here, we show the overexpression, purification, and the characterization of LAAO4 from the fungus Hebeloma cylindrosporum in the yeast Pichia pastoris with a 9His-tag and compare this with the recently characterized 6His-hcLAAO4 expressed in E. coli. The expression of the enzyme with an ER-signal sequence in P. pastoris resulted in a glycosylated, secreted protein. The enzymatic activity without activation was higher after expression in P. pastoris compared to E. coli. Due to treatment with acidic pH, a striking increase of activity could be detected for both expression systems resulting in similar specific activities after acid activation. Regarding the substrate spectrum, temperature stability, Km, and vmax values, hcLAAO4 showed very few differences when produced in these two expression systems. A higher yield of hcLAAO4 could be obtained by fermentation.
Subject(s)
Fungal Proteins/genetics , Hebeloma/enzymology , L-Amino Acid Oxidase/genetics , Enzyme Stability , Fermentation , Fungal Proteins/chemistry , Fungal Proteins/metabolism , Gene Expression , Hebeloma/chemistry , Hebeloma/genetics , Kinetics , L-Amino Acid Oxidase/chemistry , L-Amino Acid Oxidase/metabolism , Pichia/genetics , Pichia/metabolism , Recombinant Proteins/metabolism , Substrate SpecificityABSTRACT
While plants mainly rely on the use of inorganic nitrogen sources like ammonium and nitrate, soil-borne microorganisms like the ectomycorrhizal fungus Hebeloma cylindrosporum can also take up soil organic N in the form of amino acids and peptides that they use as nitrogen and carbon sources. Following the previous identification and functional expression in yeast of two PTR-like peptide transporters, the present study details the functions and substrates of HcPTR2A and HcPTR2B by analysing their transport kinetics in Xenopus laevis oocytes. While both transporters mediated high-affinity di- and tripeptide transport, HcPTR2A also showed low-affinity transport of several amino acids-mostly hydrophobic ones with large side chains.
Subject(s)
Hebeloma , Membrane Transport Proteins , Mycorrhizae , Gene Expression Regulation, Fungal , Hebeloma/genetics , Membrane Transport Proteins/geneticsABSTRACT
Hebeloma parvisporum is described as new and placed within H. sect. Porphyrospora. This mushroom is sold as an edible in markets of Laos under the local name "wai khom." Hebeloma sect. Porphyrospora is discussed and expanded to include the species formerly included in the genus Anamika and recently transferred to Hebeloma. Hebeloma sect. Porphyrospora currently comprises 16 species, 14 of which are known only from the western Pacific and Indian subcontinent. All species in this section share the character of having red-brown spores when fresh, atypical for other sections of Hebeloma, which causes the lamellae to be red-brown. However, this red-brown color fades when the material is dried. The close links, morphologically and molecularly, between H. parvisporum and other members of H. sect. Porphyrospora, particularly H. victoriense, are shown.
Subject(s)
Hebeloma/classification , Agaricales/classification , Agaricales/cytology , Agaricales/genetics , DNA, Fungal/genetics , DNA, Ribosomal Spacer/genetics , Genes, Fungal/genetics , Hebeloma/cytology , Hebeloma/genetics , Laos , Phylogeny , Pigmentation , Sequence Analysis, DNA , Spores, Fungal/classification , Spores, Fungal/cytology , Spores, Fungal/geneticsABSTRACT
Arsenic (As) contamination is one of the most daunting environmental problem bothering the whole world. Exploring a suitable bioremediation technique is an urgent need of the hour. The present study focusses on scrutinizing the ectomycorrhizal (ECM) fungus for its potential role in As detoxification and understanding the molecular mechanisms responsible for its tolerance. When exposed to increasing concentrations of external As, the ECM fungus H. cylindrosporum accumulated the metalloid intracellularly, inducing the glutathione biosynthesis pathway. The genes coding for GSH biosynthesis enzymes, γ-glutamylcysteine synthetase (Hcγ-GCS) and glutathione synthetase (HcGS) were highly regulated by As stress. Arsenic coordinately upregulated the expression of both Hcγ-GCS and HcGS genes, thus resulting in increased Hcγ-GCS and HcGS protein expressions and enzyme activities, with substantial increase in intracellular GSH. Functional complementation of the two genes (Hcγ-GCS and HcGS) in their respective yeast mutants (gsh1Δ and gsh2Δ) further validated the role of both enzymes in mitigating As toxicity. These findings clearly highlight the potential importance of GSH antioxidant defense system in regulating the As induced responses and its detoxification in ECM fungus H. cylindrosporum.
Subject(s)
Arsenic/toxicity , Glutathione/biosynthesis , Hebeloma/drug effects , Mycorrhizae/drug effects , Soil Pollutants/toxicity , Antioxidants/metabolism , Arsenic/metabolism , Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Expression Regulation, Fungal/drug effects , Genetic Complementation Test , Glutamate-Cysteine Ligase/genetics , Glutamate-Cysteine Ligase/metabolism , Glutathione/metabolism , Glutathione Synthase/genetics , Glutathione Synthase/metabolism , Hebeloma/genetics , Hebeloma/metabolism , Inactivation, Metabolic , Mutation , Mycorrhizae/genetics , Mycorrhizae/metabolism , Saccharomyces cerevisiae/metabolism , Soil Pollutants/metabolismABSTRACT
Homeostatic mechanisms preventing the toxicity of heavy metal ions in cells involve, among others, compartmentalization and binding with peptidaceous ligands, particularly the cysteinyl-rich metallothioneins (MTs). We have previously shown that in natural conditions Zn-overaccumulating ectomycorrhizal (EM) fungus Russula bresadolae stores nearly 40% of Zn bound with cysteinyl- and hystidyl-containing RaZBP peptides, which resemble MTs, while the detoxification of Zn and Cd in EM Hebeloma mesophaeum relies upon compartmentalization in small vesicles and vacuoles, respectively. Here, we examined the performance of RaZBP1 gene expressed in H. mesophaeum mycelium with respect to handling of Zn and Cd. Expression of RaZBP1 impaired growth of the mycelium on low-Zn medium by 60%, the growth was partly ameliorated upon the addition of Zn and remained considerable up to 2 mmol/L Zn, while the growth of the wild-type and control mycelia transformed with empty T-DNA was severely reduced in the presence of 0.5 mmol/L Zn; furthermore, RaZBP1 slightly added to Cd tolerance in the range of Cd concentrations of 0.625 to 8 µmol/L. Staining of Zn- or Cd-exposed hyphal cells with Zn- or Cd-specific fluorescent tracers did not indicate that the expression of RaZBP1 would redirect the flow of the metals away from their innate sinks. Size exclusion chromatography of extracted metal species revealed that the complexes corresponding to Zn/Cd-RaZBP1 are present only in minute levels. Considering that RaZBP1 inhibited growth at low Zn, and despite the benefit that it provided to H. mesophaeum in the presence of high Zn and moderate Cd, these data indicate that the binding of excess Zn and Cd with RaZBP1 is not a trait that would be outright transmitted to H. mesophaeum.
Subject(s)
Cadmium/metabolism , Fungal Proteins/metabolism , Hebeloma/metabolism , Metallothionein/metabolism , Zinc/metabolism , Basidiomycota/genetics , Cytoplasmic Vesicles/metabolism , Fungal Proteins/genetics , Hebeloma/genetics , Hebeloma/growth & development , Metallothionein/genetics , Mycelium/genetics , Mycelium/growth & development , Mycelium/metabolism , Mycorrhizae/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolismABSTRACT
Most land plants rely on root symbioses to complement or improve their mineral nutrition. Recent researches have put forward that mycorrhizal fungi efficiently absorb and transfer potassium (K+) from the soil to host plant roots, but the molecular mechanisms involved are not completely elucidated yet. We have recently revealed that K+ is likely released from the fungal Hartig net to the plant by TOK channels in the ectomycorrhizal model Hebeloma cylindrosporum - Pinus pinaster. H. cylindrosporum harbours three TOK members. Herein, we report that one of them, HcTOK1, has similar features than the yeast ScTOK1. Moreover, we propose a role for this channel in the transport of K+ from the medium to ectomycorrhizal roots under K+ starvation.
Subject(s)
Gene Expression Regulation, Fungal/physiology , Mycorrhizae/physiology , Pinus/metabolism , Pinus/microbiology , Potassium/metabolism , Symbiosis/physiology , Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Expression Regulation, Fungal/genetics , Hebeloma/genetics , Hebeloma/physiology , Homeostasis , Pinus/genetics , Symbiosis/geneticsABSTRACT
Through a mutualistic relationship with woody plant roots, ectomycorrhizal fungi provide growth-limiting nutrients, including inorganic phosphate (Pi), to their host. Reciprocal trades occur at the Hartig net, which is the symbiotic interface of ectomycorrhizas where the two partners are symplasmically isolated. Fungal Pi must be exported to the symbiotic interface, but the proteins facilitating this transfer are unknown. In the present study, we combined transcriptomic, microscopy, whole plant physiology, X-ray fluorescence mapping, 32 P labeling and fungal genetic approaches to unravel the role of HcPT2, a fungal Pi transporter, during the Hebeloma cylindrosporum-Pinus pinaster ectomycorrhizal association. We localized HcPT2 in the extra-radical hyphae and the Hartig net and demonstrated its determinant role for both the establishment of ectomycorrhizas and Pi allocation towards P. pinaster. We showed that the host plant induces HcPT2 expression and that the artificial overexpression of HcPT2 is sufficient to significantly enhance Pi export towards the central cylinder. Together, our results reveal that HcPT2 plays an important role in ectomycorrhizal symbiosis, affecting both Pi influx in the mycelium and efflux towards roots under the control of P. pinaster.
Subject(s)
Fungal Proteins/metabolism , Hebeloma/metabolism , Membrane Transport Proteins/metabolism , Mycorrhizae/physiology , Symbiosis , Fungal Proteins/genetics , Gene Expression Regulation, Fungal , Hebeloma/genetics , Hebeloma/growth & development , Membrane Transport Proteins/genetics , Models, Biological , Mycelium/metabolism , Phosphates/metabolism , Phosphorus Radioisotopes , Pinus/microbiology , Up-Regulation/geneticsABSTRACT
Ectomycorrhizal fungi play an essential role in the ecology of boreal and temperate forests through the improvement of tree mineral nutrition. Potassium (K+ ) is an essential nutrient for plants and is needed in high amounts. We recently demonstrated that the ectomycorrhizal fungus Hebeloma cylindrosporum improves the K+ nutrition of Pinus pinaster under shortage conditions. Part of the transport systems involved in K+ uptake by the fungus has been deciphered, while the molecular players responsible for the transfer of this cation towards the plant remain totally unknown. Analysis of the genome of H. cylindrosporum revealed the presence of three putative tandem-pore outward-rectifying K+ (TOK) channels that could contribute to this transfer. Here, we report the functional characterization of these three channels through two-electrode voltage-clamp experiments in oocytes and yeast complementation assays. The expression pattern and physiological role of these channels were analysed in symbiotic interaction with P. pinaster. Pine seedlings colonized by fungal transformants overexpressing two of them displayed a larger accumulation of K+ in shoots. This study revealed that TOK channels have distinctive properties and functions in axenic and symbiotic conditions and suggested that HcTOK2.2 is implicated in the symbiotic transfer of K+ from the fungus towards the plant.
Subject(s)
Mycorrhizae/metabolism , Pinus/microbiology , Potassium/metabolism , Biological Transport , Hebeloma/genetics , Minerals/metabolism , Potassium Channels , Saccharomyces cerevisiae/metabolism , Seedlings , Symbiosis/geneticsABSTRACT
To clarify the early molecular interaction between ectomycorrhizal partners, we performed a RNA-Seq study of transcriptome reprogramming of the basidiomycete Hebeloma cylindrosporum before symbiotic structure differentiation with Pinus pinaster. Mycorrhiza transcriptome was studied for comparison. By reference to asymbiotic mycelium, 47 and 46 genes were specifically upregulated over fivefold (p ≤ 0.05) upon rhizosphere colonization and root adhesion respectively. Other 45 were upregulated throughout the symbiotic interaction, from rhizosphere colonization to differentiated mycorrhizas, whereas 274 were specifically upregulated in mycorrhizas. Although exoproteome represents 5.6% of H. cylindrosporum proteome, 38.5% of the genes upregulated upon pre-infectious root colonization encoded extracellular proteins. The proportion decreased to 23.5% in mycorrhizas. At all studied time points, mycorrhiza-induced small secreted proteins (MiSSPs), representing potential effectors, were over-represented among upregulated genes. This was also the case for carbohydrate-active enzymes (CAZymes). Several CAZymes were upregulated at all studied stages of the interaction. Consistent with a role in fungal morphogenesis and symbiotic interface differentiation, CAZymes over-expressed before and upon root attachment targeted fungal and both fungal and plant polysaccharides respectively. Different hydrophobins were upregulated upon early root adhesion, in mycorrhizas or throughout interaction. The functional classification of genes upregulated only in mycorrhizas pointed to intense metabolic activity and nutritional exchanges.
Subject(s)
Hebeloma/genetics , Mycorrhizae/genetics , Symbiosis , Transcriptome , Fungal Proteins/genetics , Hebeloma/growth & development , Hebeloma/isolation & purification , Hebeloma/physiology , Mycelium/genetics , Mycelium/growth & development , Mycelium/isolation & purification , Mycorrhizae/growth & development , Mycorrhizae/isolation & purification , Mycorrhizae/physiology , Pinus/microbiology , Pinus/physiology , Plant Roots/microbiology , Plant Roots/parasitology , Proteome/genetics , Up-RegulationABSTRACT
Hebeloma section Denudata includes the majority of the taxa commonly referred to as the Hebeloma crustuliniforme complex. In a recent paper we described in detail H. subsection Denudata and fifteen European species recognised within this subsection, using morphological and molecular methods. In this paper we continue this work and describe in detail three additional subsections and several new species. Within H. subsection Hiemalia we recognise just one species, Hebeloma hiemale. Here we propose an epitype in order to unambiguously define this taxon. Nine species occurring in Europe are assigned to H. subsect. Clepsydroida, namely Hebeloma ammophilum, H. cavipes, H. fragilipes, H. ingratum, H. laetitiae, H. limbatum sp. nov., H. matritense sp. nov., H. pseudofragilipes sp. nov., and H. vaccinum. Finally, we introduce H. subsection Echinospora with three species: Hebeloma echinosporum sp. nov., H. populinum, and H. rostratum sp. nov. We provide descriptions of all three of these species in order to clarify the taxonomy of this section. We provide a key to H. sect. Denudata and the discussed subsections. For the majority of the taxa there is good overall consistency between morphological and phylogenetic delimitation and, where the information exists, thanks to Aanen and Kuyper's work, biological delimitation.
Subject(s)
Hebeloma/classification , Phylogeny , Europe , Hebeloma/genetics , Hebeloma/growth & development , Hebeloma/isolation & purification , Molecular Sequence Data , Spores, Fungal/classification , Spores, Fungal/genetics , Spores, Fungal/growth & development , Spores, Fungal/isolation & purificationABSTRACT
Between 2002 and 2012 regular visits to the Carpathians were made and a number of Hebeloma spp. were collected from the alpine area. In total 44 collections were made that represent 11 species, two of which, Hebeloma grandisporum and H. oreophilum, are described here as new. Of the 11 species, four (H. alpinum, H. marginatulum and the two species described as new) are known only from alpine or Arctic habitats. Hebeloma dunense and H. mesophaeum are commonly found in, but not restricted to, alpine habitats. The other five species (H. aanenii, H. laterinum, H. naviculosporum, H. vaccinum, H. velutipes) are usually found in lowland or boreal habitats. Hebeloma naviculosporum is reported for the first time from the alpine zone and H. alpinum for the first time as growing with Helianthemum. All but two species (H. alpinum, H. marginatulum) are reported for the first time from the Carpathian alpine zone. In this paper we discuss the habitat, the 11 recorded species and give detailed descriptions of the two new species, both morphologically and molecularly. A key for Hebeloma species from sect. Hebeloma occurring in Arctic-alpine habitats is provided.
Subject(s)
Hebeloma/classification , Hebeloma/isolation & purification , Ecosystem , Europe , Fungal Proteins/genetics , Hebeloma/genetics , Hebeloma/growth & development , Molecular Sequence Data , Peptide Elongation Factor 1/genetics , Phylogeny , RNA Polymerase II/genetics , Soil Microbiology , Spores, Fungal/classification , Spores, Fungal/genetics , Spores, Fungal/growth & development , Spores, Fungal/isolation & purificationABSTRACT
Extracellular proteins play crucial roles in the interaction between mycorrhizal fungi and their environment. Computational prediction and experimental detection allowed identification of 869 proteins constituting the exoproteome of Hebeloma cylindrosporum. Small secreted proteins (SSPs) and carbohydrate-active enzymes (CAZymes) were the two major classes of extracellular proteins. Twenty-eight per cent of the SSPs were secreted by free-living mycelia and five of the 10 most abundant extracellular proteins were SSPs. By contrast, 63-75% of enzymes involved in nutrient acquisition were secreted. A total of 150 extracellular protein-coding genes were differentially expressed between mycorrhizas and free-living mycelia. SSPs were the most affected. External environmental conditions also affected expression of 199 exoproteome genes in mycorrhizas. SSPs displayed different patterns of regulation in response to presence of a host plant or other environmental signals. Several of the genes most overexpressed in the presence of organic matter encoded oxidoreductases. Hebeloma cylindrosporum has not fully lost its ancestral saprotrophic capacities but rather adapted them not to harm its hosts and to use soil organic nitrogen. The complex and divergent patterns of regulation of SSPs in response to a symbiotic partner and/or organic matter suggest various roles in the biology of mycorrhizal fungi.
Subject(s)
Fungal Proteins/metabolism , Genes, Fungal , Hebeloma/metabolism , Mycorrhizae/metabolism , Proteome/metabolism , Symbiosis , Fungal Proteins/genetics , Genomics , Hebeloma/genetics , Proteomics , TranscriptomeABSTRACT
Hebeloma ifeleletorum is described as a new species from American Samoa. Based on analyses of ITS and combined nLSU-ITS datasets H. ifeleletorum clusters with but is distinct from described species that have been placed in the genus Anamika by some. The phylogenetic relationship of H. ifeleletorum to the genus Anamika from Asia and to other species from Australia and New Caledonia suggests that H. ifeleletorum has origins in the western Pacific.
Subject(s)
Hebeloma/classification , Hebeloma/isolation & purification , Phylogeny , Asia , Australia , DNA, Fungal/genetics , Hebeloma/genetics , Hebeloma/growth & development , Molecular Sequence Data , Spores, Fungal/classification , Spores, Fungal/genetics , Spores, Fungal/growth & development , Spores, Fungal/isolation & purificationABSTRACT
We used Agrobacterium-mediated insertional mutagenesis to identify genes in the ectomycorrhizal fungus Hebeloma cylindrosporum that are essential for efficient mycorrhiza formation. One of the mutants presented a dramatically reduced ability to form ectomycorrhizas when grown in the presence of Pinus pinaster. It failed to form mycorrhizas in the presence of glucose at 0.5 g liter(-1), a condition favorable for mycorrhiza formation by the wild-type strain. However, it formed few mycorrhizas when glucose was replaced by fructose or when glucose concentration was increased to 1 g liter(-1). Scanning electron microscopy examination of these mycorrhizas revealed that this mutant was unable to differentiate true fungal sheath and Hartig net. Molecular analyses showed that the single-copy disrupting T-DNA was integrated 6,884 bp downstream from the start codon, of an open reading frame potentially encoding a 3,096-amino-acid-long protein. This gene, which we named HcMycE1, has orthologs in numerous fungi as well as different other eukaryotic microorganisms. RNAi inactivation of HcMycE1 in the wild-type strain also led to a mycorrhizal defect, demonstrating that the nonmycorrhizal phenotype of the mutant was due to mutagenic T-DNA integration in HcMycE1. In the wild-type strain colonizing P. pinaster roots, HcMycE1 was transiently upregulated before symbiotic structure differentiation. Together with the inability of the mutant to differentiate these structures, this suggests that HcMycE1 plays a crucial role upstream of the fungal sheath and Hartig net differentiation. This study provides the first characterization of a fungal mutant altered in mycorrhizal ability.
Subject(s)
Fungal Proteins/metabolism , Gene Expression Regulation, Fungal , Hebeloma/genetics , Mycorrhizae/genetics , Pinus/microbiology , Fungal Proteins/genetics , Hebeloma/physiology , Hebeloma/ultrastructure , Microscopy, Electron, Scanning , Multigene Family , Mutagenesis, Insertional , Mycelium , Mycorrhizae/physiology , Mycorrhizae/ultrastructure , Phenotype , Phylogeny , Pinus/ultrastructure , Plant Roots/microbiology , Plant Roots/ultrastructure , RNA Interference , SymbiosisABSTRACT
Mycorrhizal associations are known to improve the hydro-mineral nutrition of their host plants. However, the importance of mycorrhizal symbiosis for plant potassium nutrition has so far been poorly studied. We therefore investigated the impact of the ectomycorrhizal fungus Hebeloma cylindrosporum on the potassium nutrition of Pinus pinaster and examined the involvement of the fungal potassium transporter HcTrk1. HcTrk1 transcripts and proteins were localized in ectomycorrhizas using in situ hybridization and EGFP translational fusion constructs. Importantly, an overexpression strategy was performed on a H. cylindrosporum endogenous gene in order to dissect the role of this transporter. The potassium nutrition of mycorrhizal pine plants was significantly improved under potassium-limiting conditions. Fungal strains overexpressing HcTrk1 reduced the translocation of potassium and phosphorus from the roots to the shoots of inoculated plants in mycorrhizal experiments. Furthermore, expression of HcTrk1 and the phosphate transporter HcPT1.1 were reciprocally linked to the external inorganic phosphate and potassium availability. The development of these approaches provides a deeper insight into the role of ectomycorrhizal symbiosis on host plant K(+) nutrition and in particular, the K(+) transporter HcTrk1. The work augments our knowledge of the link between potassium and phosphorus nutrition via the mycorrhizal pathway.
Subject(s)
Fungal Proteins/metabolism , Hebeloma/metabolism , Host-Pathogen Interactions , Mycorrhizae/metabolism , Phosphorus/metabolism , Pinus/metabolism , Potassium/metabolism , Biological Transport/drug effects , DNA, Bacterial/genetics , Gene Expression Regulation, Fungal/drug effects , Hebeloma/drug effects , Hebeloma/genetics , Host-Pathogen Interactions/drug effects , Hyphae/drug effects , Hyphae/metabolism , Mycorrhizae/drug effects , Mycorrhizae/genetics , Phenotype , Phosphorus/pharmacology , Pinus/drug effects , Pinus/microbiology , Potassium/pharmacology , RNA, Messenger/genetics , RNA, Messenger/metabolism , Seedlings/drug effects , Seedlings/metabolism , Seedlings/microbiology , Sodium/metabolismABSTRACT
Mycorrhizal exchange of nutrients between fungi and host plants involves a specialization and polarization of the fungal plasma membrane adapted for the uptake from the soil and for secretion of nutrient ions towards root cells. In addition to the current progress in identification of membrane transport systems of both symbiotic partners, data concerning the transcriptional and translational regulation of these proteins are needed to elucidate their role for symbiotic functions. To answer whether the formerly described Pi-dependent expression of the phosphate transporter HcPT1.1 from Hebeloma cylindrosporum is the result of its promoter activity, we introduced promoter-EGFP fusion constructs in the fungus by Agrotransformation. Indeed, HcPT1.1 expression in pure fungal cultures quantified and visualized by EGFP under control of the HcPT1.1 promoter was dependent on external Pi concentrations, low Pi stimulating the expression. Furthermore, to study expression and localization of the phosphate transporter HcPT1.1 in symbiotic conditions, presence of transcripts and proteins was analyzed by the in situ hybridization technique as well as by immunostaining of proteins. In ectomycorrhiza, expression of the phosphate transporter was clearly enhanced by Pi-shortage indicating its role in Pi nutrition in the symbiotic association. Transcripts were detected in external hyphae and in the hyphal mantle, proteins in addition also within the Hartig net. Exploiting the transformable fungus H. cylindrosporum, Pi-dependent expression of the fungal transporter HcPT1.1 as result from its promoter activity as well as transcript and protein localization in ectomycorrhizal symbiosis are shown.
Subject(s)
Fungal Proteins/genetics , Fungal Proteins/metabolism , Gene Expression Regulation, Fungal , Mycorrhizae/metabolism , Phosphate Transport Proteins/genetics , Phosphate Transport Proteins/metabolism , Phosphates/metabolism , Promoter Regions, Genetic , Hebeloma/genetics , Hebeloma/metabolism , Hyphae/genetics , Hyphae/growth & development , Hyphae/metabolism , Mycorrhizae/genetics , Mycorrhizae/growth & development , Pinus/microbiology , Pinus/physiology , Protein Transport , SymbiosisABSTRACT
The first collection of a macrofungal agaric species, with morphological features similar to already described Anamika species, has been found in association with animal bones in north Queensland, Australia. This species also shares features with several, commonly occurring and previously described Australian Hebeloma species. An integrated morphological and molecular study has resulted in the conclusion that all Anamika species belong in Hebeloma. As a result, already described species of Anamika are recombined as H. indicum (K.A. Thomas, Peintner, M.M. Moser and Manim.) B.J. Rees & Orlovich, H. angustilamellatum (Zhu L. Yang and Z.W. Ge) B.J. Rees & Orlovich and H. lactariolens (Clémençon and Hongo) B.J. Rees & Orlovich. A. phylogenetic tree based on ribosomal ITS sequences examines the relationship of these species with other Hebeloma species from both hemispheres. Four new species, Hebeloma youngii B.J. Rees, H. nothofagetorum B.J. Rees, H. subvictoriense B.J. Rees, H. lacteocoffeatum B.J. Rees, and one form, H. aminophilum f. hygrosarx B.J. Rees, are described as new from Australia.
Subject(s)
Hebeloma/classification , Australia , Hebeloma/genetics , Hebeloma/ultrastructure , Microscopy, Electron, Scanning , PhylogenyABSTRACT
Nitrogen (N) utilization by ectomycorrhizal fungi is an essential aspect of their ecosystem function. N deposition changes both the N pools and the carbon/nitrogen (C/N) ratio of the substrates where ectomycorrhizal fungi are found, and it is important to understand how these changes affect the N forms used by ectomycorrhizal fungi. To overcome the difficulties of studying ectomycorrhizal fungi in situ, we investigated all known N genes in the model fungus, Hebeloma cylindrosporum in a culture study. In addition to studying the regulation of all known N utilization genes, we aimed to understand whether there are gene clusters that undergo similar regulation. Lastly we studied how C/N ratio, N transporter type, and N source affected relative gene expression levels. We grew the D2 strain of H. cylindrosporum on a range of inorganic and organic N sources under low, medium, and high C/N ratios. We found three gene clusters that were regulated in a similar pattern. Lastly, we found C/N ratio, N source and N transporter type all affected gene expression levels. Relative expression levels were highest on the high C/N ratio, BSA and diLeucine N sources, and inorganic N transporters were always expressed at higher levels than organic N transporters. These results suggest that inorganic N sources may always the default preference for H. cylindrosporum, regardless of both the N sources and the C/N ratio of the substrate.
Subject(s)
Carbon/metabolism , Gene Expression Regulation, Fungal , Genes, Fungal , Hebeloma/genetics , Nitrogen/metabolism , Amino Acids/metabolism , Biological Transport , Culture Media/metabolism , Fungal Proteins/genetics , Fungal Proteins/metabolism , Hebeloma/growth & development , Hebeloma/metabolism , Microbiological Techniques , Multigene Family , Mycorrhizae/genetics , Mycorrhizae/growth & development , Mycorrhizae/metabolismABSTRACT
Catabolism of amino acids is a central process in cellular nitrogen turnover, but only a few of the mechanisms involved have been described from basidiomycete fungi. This study identified one such mechanism, the l-amino acid oxidase (Lao1) enzyme of Hebeloma cylindrosporum, by 2D gel separation and MS. We determined genomic DNA sequences of lao1 and part of its upstream gene, a putative pyruvate decarboxylase (pdc2), and cloned the cDNA of lao1. The two genes were also identified and annotated from the genome of Laccaria bicolor. The lao1 and pdc2 gene structures were conserved between the two fungi. The intergenic region of L. bicolor possessed putative duplications not detected in H. cylindrosporum. Lao1 sequences possessed dinucleotide-binding motifs typical for flavoproteins. Lao1 was less than 23â% identical to Lao sequences described previously. Recombinant Lao1 of H. cylindrosporum was expressed in Escherichia coli, purified and refolded with SDS to gain catalytic activity. The enzyme possessed broad substrate specificity: 37 l-amino acids or derivatives served as effective substrates. The highest activities were recorded with l-glutamate, but positively charged and aromatic amino acids were also accepted. Michaelis constants for six amino acids varied from 0.5 to 6.7 mM. We have thus characterized a novel type of Lao-enzyme and its gene from the basidiomycete fungus H. cylindrosporum.
Subject(s)
Fungal Proteins/chemistry , Glutamic Acid/metabolism , Hebeloma/enzymology , L-Amino Acid Oxidase/chemistry , Amino Acid Sequence , Fungal Proteins/genetics , Fungal Proteins/metabolism , Hebeloma/chemistry , Hebeloma/genetics , L-Amino Acid Oxidase/genetics , L-Amino Acid Oxidase/metabolism , Molecular Sequence Data , Sequence Alignment , Substrate SpecificityABSTRACT
Metal tolerance of filamentous fungi is a poorly understood mechanism. In order to unravel the molecular basis of zinc (Zn) tolerance in the ectomycorrhizal fungal model Hebeloma cylindrosporum, we carried out a functional screening of an H. cylindrosporum cDNA library in the zrc1Δ mutant strain of Saccharomyces cerevisiae to search for genes conferring Zn tolerance to yeast cells. This strategy allowed the isolation of HcZnT1, a gene belonging to the cation diffusion facilitator family, which induced tolerance to Zn, but not to other metals. HcZnT1 was constitutively expressed in Hebeloma cells, whatever the Zn status of the medium and the fungal cell type (mycelia, sporocarps, mycorrhizas). A HcZnT1:GFP fusion protein was expressed in yeast and the corresponding fluorescence was recorded on endoplasmic reticulum membranes. Taken together, these different findings suggest a dual role of HcZnT1 in Zn homeostasis of fungal cells, by supplying requested Zn ions for the functioning of the endoplasmic reticulum as well as by detoxifying the cytosol under Zn stress. Zn pools were also investigated by using the Zn-specific fluorophore zinquin in H. cylindrosporum cells. Zinquin labeling revealed compartmentalization in intracellular vesicles interspersed throughout the cytoplasm that do not correspond to vacuolar compartments. Altogether the present data represent the first steps into the understanding of Zn homeostasis and tolerance in Hebeloma.