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1.
Nat Rev Microbiol ; 21(9): 573-589, 2023 09.
Article in English | MEDLINE | ID: mdl-37185947

ABSTRACT

Infectious hepatitis type A and type E are caused by phylogenetically distinct single-stranded, positive-sense RNA viruses that were once considered to be non-enveloped. However, studies show that both are released nonlytically from hepatocytes as 'quasi-enveloped' virions cloaked in host membranes. These virion types predominate in the blood of infected individuals and mediate virus spread within the liver. They lack virally encoded proteins on their surface and are resistant to neutralizing anti-capsid antibodies induced by infection, yet they efficiently enter cells and initiate new rounds of virus replication. In this Review, we discuss the mechanisms by which specific peptide sequences in the capsids of these quasi-enveloped virions mediate their endosomal sorting complexes required for transport (ESCRT)-dependent release from hepatocytes through multivesicular endosomes, what is known about how they enter cells, and the impact of capsid quasi-envelopment on host immunity and pathogenesis.


Subject(s)
Liver , Virus Internalization , Humans , Capsid Proteins , Capsid/metabolism , Hepatitis Viruses/metabolism , Virion/metabolism
2.
Medicine (Baltimore) ; 101(42): e31280, 2022 Oct 21.
Article in English | MEDLINE | ID: mdl-36281156

ABSTRACT

BACKGROUND: The study aims to provide a comprehensive account of the association between the epidermal growth factor (EGF) + 61A/G polymorphism (rs4444903) and susceptibility to virus-related hepatocellular carcinoma (HCC). METHODS: Electronic searching of the Chinese National Knowledge Infrastructure, Wanfang, Chinese Scientific Journal Database (VIP), PubMed, Web of Science, and Embase was conducted to select eligible studies. Odds ratios (OR) and 95% confidence intervals (95%CI) were calculated to assess the strength of the association. RESULTS: In this study, a total of 18 articles were included with 2692 cases and 5835 controls for assessing the association between rs4444903 and HCC risk. The pooled results showed that the EGF + 61A/G polymorphism was significantly associated with the risk of virus-related HCC in all genetic models. Stratified analyses were conducted based on ethnicity, study quality, source of controls, type of controls, number of cases and genotyping method. The results showed that EGF + 61A/G polymorphisms significantly affect HCC susceptibility in different stratified populations. High heterogeneity was observed across included studies, and meta-regression analysis demonstrated that race, type of controls, and study quality contribute to the observed heterogeneity. CONCLUSION: This pooled analysis found that EGF + 61A/G polymorphism was significantly associated with the risk of HCC.


Subject(s)
Carcinoma, Hepatocellular , Hepatitis , Liver Neoplasms , Humans , Carcinoma, Hepatocellular/pathology , Epidermal Growth Factor/genetics , Epidermal Growth Factor/metabolism , Liver Neoplasms/pathology , Genetic Predisposition to Disease , Risk Factors , Hepatitis Viruses/metabolism , Polymorphism, Single Nucleotide
3.
Transfusion ; 59(10): 3177-3185, 2019 10.
Article in English | MEDLINE | ID: mdl-31393615

ABSTRACT

BACKGROUND: A serum alanine aminotransferase (ALT) test is currently demanded for blood donation in China. One of the major reasons to include such a test is possible etiology of known or unknown hepatotropic viruses. However, this hypothesis has never been examined convincingly. STUDY DESIGN AND METHODS: The study recruited 90 Chinese blood donors that were divided into three groups based on their ALT values. Serum virome from these donors was explored using a metagenomics approach with enhanced sensitivity resolved at single sequencing reads. RESULTS: Anellovirus and pegivirus C (GBV-C) were detected among these donors. None of them were found solely in donors with abnormal liver enzyme. Anellovirus was highly prevalent (93.3%) and the co-infection with multiple genera (alpha, beta, and gammatorquevirus) were more common in the donors with normal ALT values in comparison to those with elevated ALT (single/double/triple Anellovirus genera, 1/3/24 vs. 7/7/14 or 6/7/13, p = 0.009). For unmapped reads that accounted for 15 ± 14.9% of the data, similarity-based (BLASTN, BLASTP, and HMMER3) and similarity-independent (k-mer frequency) analysis identified several circular rep encoding ssDNA (CRESS-DNA) genomes. Direct PCR testing indicated these genomes were likely reagent contaminants. CONCLUSION: Viral etiology is not responsible for elevated ALT levels in Chinese blood donors. The ALT test, if not abandoned, should be adjusted for its cutoff in response to donor shortage in China.


Subject(s)
Alanine Transaminase/blood , Anelloviridae , Blood Donors , Genome, Viral , Hepatitis Viruses , Hepatitis, Viral, Human , Adult , Anelloviridae/genetics , Anelloviridae/metabolism , Asian People , China/epidemiology , Female , Hepatitis Viruses/genetics , Hepatitis Viruses/metabolism , Hepatitis, Viral, Human/blood , Hepatitis, Viral, Human/epidemiology , Hepatitis, Viral, Human/genetics , Humans , Male , Middle Aged , Prevalence
4.
Proteomics ; 15(22): 3815-25, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26314548

ABSTRACT

Hepatitis C virus (HCV) infection often leads to chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. The stability of the HCV proteins is controlled by ubiquitin-dependent and ubiquitin-independent proteasome pathways. Many viruses modulate proteasome function for their propagation. To examine the interrelationship between HCV and the proteasome pathways we employed a quantitative activity-based protein profiling method. Using this approach we were able to quantify the changes in the activity of several proteasome subunits and found that proteasome activity is drastically reduced by HCV replication. The results imply a link between the direct downregulation of the activity of this pathway and chronic HCV infection.


Subject(s)
Hepacivirus/physiology , Proteasome Endopeptidase Complex/metabolism , Cell Line, Tumor , Hepacivirus/drug effects , Hepatitis C/metabolism , Hepatitis C/virology , Hepatitis Viruses/drug effects , Hepatitis Viruses/metabolism , Humans , Isoenzymes/metabolism , Lactones/pharmacology , Orlistat , Proteome/metabolism , Signal Transduction , Viral Proteins/metabolism , Virus Replication
5.
Mol Biol Rep ; 39(2): 1217-23, 2012 Feb.
Article in English | MEDLINE | ID: mdl-21598110

ABSTRACT

Acute and chronic viral hepatitis infections are corresponding to increase the risk of different types of hematological malignancies especially with leukemia. In this study the serological and molecular markers of hepatitis viruses were evaluated in patients with different types of leukemia in comparing with control group. In this cross sectional study, 100 EDTA-treated blood samples were collected from leukemia patients and also from healthy control group, respectively. Serological and molecular markers of HBV, HCV and HDV viruses were analyzed for determination of the role of these hepatitis viruses in clinical outcomes of leukemia disorders. Increasing risk factors of leukemia were evaluated statistically in two studied groups by SPSS software. One of molecular and immunological markers of HBV, HDV and HCV was found in 24 of 100 (24%), 22 of 100 (22%), and 1 of 100 (1%) patients with leukemia and in 12 of 100 (12%), 6 of 100 (6%), and 2 of 100 (2%) control patients. Significant differences were detected in detection of HBsAg (P = 0.02), HBeAb (P = 0.009), and HCV-RNA (P = 0.05) between leukemia patients and control group, respectively. The high prevalence of HBV and HCV infective markers were detected in ALL and AML patients. Identification of high prevalence of HBV and HCV infective markers in leukemia patients proposed strong association between hepatitis viral infections and leukemia. Therefore, evaluation of the prevalence of viral hepatitis infections in larger groups of patients with long lasting follow up is suggesting.


Subject(s)
Biomarkers, Tumor/metabolism , Hematologic Neoplasms/epidemiology , Hematologic Neoplasms/virology , Hepatitis Viruses/metabolism , Biomarkers, Tumor/blood , Cross-Sectional Studies , Humans , Iran/epidemiology , Prevalence , Risk Factors
6.
Georgian Med News ; (194): 84-90, 2011 May.
Article in Russian | MEDLINE | ID: mdl-21685531

ABSTRACT

In present review hepatocyte apoptosis is presented as universal defensive reaction of liver, to the damages. Hepatocyte apoptosis may be caused by hepatotropic virus's direct affection, or by the immune reactions initiated by viruses. Apoptosis development caused by virus direct affection varies and contains at lest two mechanisms: production of specific proteins: B virus - X protein and C virus - core-protein; expression of the receptors leading the induction of this process on the hepatocyte membrane, for example, increasing of Fas-receptor and cell sensation to apoptosis stimulus. In apoptosis induced by immune reaction T-lymphocytes could trigger off apoptosis in two principal ways: by releasing perporines that produce holes through hepatocyte membrane and according to this process granzyms are permetted inside the cells. By destroying of caspases by proteases that initiate apoptosis cascade. In this article molecular mechanisms of the processes mentioned above are also discussed.


Subject(s)
Apoptosis , Hepatitis Viruses/metabolism , Hepatitis/pathology , Liver/pathology , Liver/virology , Caspases/metabolism , Hepatitis/metabolism , Hepatocytes/pathology , Hepatocytes/virology , Humans , T-Lymphocytes/metabolism , Viral Proteins/metabolism , fas Receptor/genetics , fas Receptor/metabolism
8.
Lab Invest ; 90(12): 1690-703, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20697376

ABSTRACT

Hedgehog (Hh) pathway activation promotes many processes that occur during fibrogenic liver repair. Whether the Hh pathway modulates the outcomes of virally mediated liver injury has never been examined. Gene-profiling studies of human hepatocellular carcinomas (HCCs) demonstrate Hh pathway activation in HCCs related to chronic infection with hepatitis B virus (HBV) or hepatitis C virus (HCV). Because most HCCs develop in cirrhotic livers, we hypothesized that Hh pathway activation occurs during fibrogenic repair of liver damage due to chronic viral hepatitis, and that Hh-responsive cells mediate disease progression and hepatocarciongenesis in chronic viral hepatitis. Immunohistochemistry and qRT-PCR analysis were used to analyze Hh pathway activation and identify Hh-responsive cell types in liver biopsies from 45 patients with chronic HBV or HCV. Hh signaling was then manipulated in cultured liver cells to directly assess the impact of Hh activity in relevant cell types. We found increased hepatic expression of Hh ligands in all patients with chronic viral hepatitis, and demonstrated that infection with HCV stimulated cultured hepatocytes to produce Hh ligands. The major cell populations that expanded during cirrhosis and HCC (ie, liver myofibroblasts, activated endothelial cells, and progenitors expressing markers of tumor stem/initiating cells) were Hh responsive, and higher levels of Hh pathway activity associated with cirrhosis and HCC. Inhibiting pathway activity in Hh-responsive target cells reduced fibrogenesis, angiogenesis, and growth. In conclusion, HBV/HCV infection increases hepatocyte production of Hh ligands and expands the types of Hh-responsive cells that promote liver fibrosis and cancer.


Subject(s)
Carcinoma, Hepatocellular/metabolism , Carcinoma, Hepatocellular/virology , Hedgehog Proteins/metabolism , Hepatitis C, Chronic/complications , Liver Cirrhosis/complications , Liver Neoplasms/metabolism , Liver Neoplasms/virology , Viral Proteins/pharmacology , Adult , Aged , Carcinoma, Hepatocellular/pathology , Cell Line, Tumor , Cells, Cultured , Disease Progression , Female , Hepatitis C, Chronic/pathology , Hepatitis Viruses/metabolism , Hepatocytes/metabolism , Hepatocytes/pathology , Humans , Liver/metabolism , Liver/pathology , Liver Cirrhosis/pathology , Liver Neoplasms/pathology , Male , Middle Aged , Signal Transduction
9.
Biochem J ; 419(1): 133-9, 2009 Apr 01.
Article in English | MEDLINE | ID: mdl-18973474

ABSTRACT

A panel of 52 murine monoclonal antibodies was found to recognize antigenic determinants that had been conserved among all major genetic subgroups of the H5N1 avian influenza virus prevalent since 1997. We screened a phage display library for peptides recognized by one such antibody (8H5). We analysed the specificity of 8H5 for reactive peptides presented as fusion proteins of HBc (hepatitis B core protein) and HEV (hepatitis E virus) structural protein, p239. This was then related to the specificity of the native HA (haemagglutinin) molecule by virtue of the capacity of fusion proteins to compete for 8H5 binding with different strains of H5N1 virus and the reactivity of antisera generated against fusion proteins to bind native HA molecules, and to inhibit haemagglutination and arrest infection by the virus. Nine reactive peptides of different amino acid sequences were identified, six of which were also reactive with the antibody in association with HBc and four were in association with p239. Binding occurred with the dimeric form of the four p239-fusion proteins and one of the HBc-fusion proteins, but not with the monomeric form. The HBc-fusion proteins blocked 8H5 binding with four strains of H5N1 influenza virus. Mouse antisera generated against fusion proteins bound to HA molecules, but did not inhibit haemagglutination or arrest H5N1 infection. Our findings indicate that 8H5 recognizes discontinuous sites presented by secondary and possibly higher structural orders of the peptides in spatially favourable positions for binding with the antibody, and that the peptides partially mimic the native 8H5 epitopes on the H5N1 virus.


Subject(s)
Influenza A Virus, H5N1 Subtype/immunology , Influenza A Virus, H5N1 Subtype/metabolism , Peptides/immunology , Peptides/metabolism , Animals , Antibodies, Monoclonal/immunology , Blotting, Western , Cell Line , Enzyme-Linked Immunosorbent Assay , Flow Cytometry , Hemagglutination , Hepatitis B Core Antigens/genetics , Hepatitis B Core Antigens/immunology , Hepatitis B Core Antigens/metabolism , Hepatitis Viruses/genetics , Hepatitis Viruses/immunology , Hepatitis Viruses/metabolism , Immune Sera/immunology , Influenza A Virus, H5N1 Subtype/genetics , Peptide Library , Peptides/genetics , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology , Recombinant Fusion Proteins/metabolism , Spodoptera , Viral Proteins/genetics , Viral Proteins/immunology , Viral Proteins/metabolism
10.
Eur J Pediatr ; 168(4): 469-76, 2009 Apr.
Article in English | MEDLINE | ID: mdl-18560888

ABSTRACT

Biliary atresia (BA) is the most frequent indication for paediatric liver transplantation. We tested the hypothesis of a viral aetiology of this disease by screening liver samples of a large number of BA patients for the common human hepatotropic viruses. Moreover, we correlated our findings to the expression of Mx protein, which has been shown to be significantly up-regulated during viral infections. Seventy-four liver biopsies (taken during Kasai portoenterostomy) were tested by polymerase chain reaction (PCR) for DNA viruses (herpes simplex virus [HSV], Epstein-Barr virus [EBV], varicella zoster virus [VZV], cytomegalovirus [CMV], adenovirus, parvovirus B19 and polyoma BK) and RNA viruses (enteroviruses, rotavirus and reovirus 3). Mx protein expression was assessed by immunohistochemistry. Virus DNA/RNA was found in less than half of the biopsies (8/74 CMV, 1/74 adenovirus; 21/64 reovirus, 1/64 enterovirus). A limited number presented with double infection. Patients that had detectable viral RNA/DNA in their liver biopsies were significantly older than virus-free patients (P = 0.037). The majority (54/59) of the liver biopsies showed expression of Mx proteins in hepatocytes, bile ducts and epithelium. Our data suggest that the known hepatotropic viruses do not play a major role in the aetiology and progression of BA. Their incidence appears to be, rather, a secondary phenomenon. Nonetheless, the inflammatory response in the livers of BA patients mimics that observed during viral infections.


Subject(s)
Biliary Atresia/virology , Hepatitis Viruses/isolation & purification , Hepatitis, Viral, Human/epidemiology , Hepatitis, Viral, Human/virology , Adenoviridae/isolation & purification , Biliary Atresia/epidemiology , Biomarkers/metabolism , Comorbidity , Cytomegalovirus/isolation & purification , DNA, Viral/isolation & purification , Enterovirus/isolation & purification , Female , GTP-Binding Proteins/metabolism , Hepatitis Viruses/metabolism , Hepatitis, Viral, Human/metabolism , Herpesvirus 3, Human/isolation & purification , Herpesvirus 4, Human/isolation & purification , Humans , Incidence , Infant , Infant, Newborn , Liver/pathology , Liver/virology , Male , Mammalian orthoreovirus 3/isolation & purification , Myxovirus Resistance Proteins , Parvovirus B19, Human/isolation & purification , Polyomavirus/isolation & purification , RNA, Viral/isolation & purification , Retrospective Studies , Rotavirus/isolation & purification , Simplexvirus/isolation & purification
11.
Biochem Biophys Res Commun ; 341(2): 314-9, 2006 Mar 10.
Article in English | MEDLINE | ID: mdl-16426578

ABSTRACT

Apolipoprotein B mRNA-editing enzyme catalytic-polypeptide 3G (APOBEC3G) is a potent inhibitor of infection by a wide range of retroviruses. Although recent reports have suggested that human APOBEC3G exerts antiviral activity against hepatitis B virus, APOBEC3G expression is normally low in the human liver. To clarify the role of APOBEC3G in cellular defenses against hepatitis viruses, the regulation of the APOBEC3G expression was investigated in human hepatocytes. Endogenous transcripts of nine APOBEC family members were barely detectable in quiescent liver cells. However, APOBEC3G was significantly up-regulated in response to interferon-alpha (IFN-alpha) stimulation in HepG2, Huh-7, and primary human hepatocytes. IFN regulatory factor elements that are important for IFN-inducible promoter activity were identified 5' upstream from the human APOBEC3G gene. Our findings provided the first evidence showing that APOBEC3G is induced by IFN stimulation in human hepatocytes and thus could be involved in host defense mechanisms directed against hepatitis viruses.


Subject(s)
Antiviral Agents/chemistry , Interferon-alpha/metabolism , Nucleoside Deaminases/physiology , Repressor Proteins/physiology , APOBEC-3G Deaminase , Base Sequence , Carcinoma, Hepatocellular/metabolism , Cell Line , Cytidine Deaminase , DNA Primers/chemistry , Gene Expression Regulation , Hepatitis Viruses/metabolism , Hepatocytes/metabolism , Humans , Immunoblotting , Luciferases/metabolism , Models, Genetic , Molecular Sequence Data , Nucleoside Deaminases/metabolism , Plasmids/metabolism , Promoter Regions, Genetic , RNA/metabolism , Repressor Proteins/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Time Factors
12.
Brief Funct Genomic Proteomic ; 4(3): 225-40, 2005 Nov.
Article in English | MEDLINE | ID: mdl-16420748

ABSTRACT

Laboratory mice often exhibit wide differences in susceptibility when infected experimentally with viruses. Based on such observations, experiments have been designed to investigate the determinism of these differences at the molecular level, and a few genes that play a major role in the innate mechanisms of defence of the species toward viral aggressions have been characterised. For example, the extraordinary resistance of SJL mice to experimental infections with hepatitis virus strain A59 is the consequence of a structural alteration of a cell adhesion molecule which normally binds to the spikes of the virus, allowing its entry into the cells. If the virus cannot bind to the molecule, or if the molecule is absent, epithelial cells of the intestine and liver are not infected and mice are resistant. In the same way, most--not to say all--laboratory strains of mice are susceptible to infections with orthomyxoviruses or flaviviruses because essential molecules, the synthesis of which is normally triggered by interferon, are defective in these mice. Wild mice, by contrast --probably because they are constantly exposed to natural infections--are resistant. Finally, some mouse strains resist experimental infections by the mouse cytomegalovirus 1 (MCMV-1) because, once infected, these mice synthesise a molecule at the surface of infected cells which allows immediate recognition and killing by natural killer (NK) cells. With the exuberant development of mouse genetics and the constant generation of new mutant alleles, it is likely that many more genes with an impact on the phenotype of resistance or susceptibility will be identified in the forthcoming years. These genes are probably numerous, however, and many of them presumably interact with each other and/or have additive effects. This might slow down progress in our understanding of the innate mechanism of defence.


Subject(s)
Genetic Predisposition to Disease , Virus Diseases/genetics , Amino Acid Motifs , Animals , Bunyaviridae/metabolism , Coronavirus/metabolism , Flavivirus/metabolism , Genome , Hepatitis Viruses/metabolism , Herpesviridae/metabolism , Humans , Killer Cells, Natural/metabolism , Killer Cells, Natural/virology , Mice , Mice, Inbred Strains , Models, Genetic , Muromegalovirus/metabolism , Orthomyxoviridae/metabolism , Retroviridae/genetics , Species Specificity
13.
Med Sci Monit ; 8(11): CR762-6, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12444381

ABSTRACT

BACKGROUND: Hepatitis A and B can be prevented by active immunization. The purpose of our study was to estimate the prevalence of viral markers of hepatitis A, B, and C among medical students, to assess the efficacy of vaccination against HBV infection after 4 years, and to evaluate the efficacy of vaccination against HAV infection. MATERIAL/METHODS: The study group consisted of 150 medical students, who were vaccinated with Engerix B at the beginning of medical studies. From this population we identified a subgroup of 45 students, in whom the presence of anti-HAV was not confirmed; they were then vaccinated with HAVRIX 1440. Viral markers for HAV, HBV, HCV were determined in this group. RESULTS: In the group of 150 persons, we found anti-HBc in 8 students (5.3%), and HBsAg in 1 (0.7%), who did not have either HBeAg or anti-HBe. Anti-HBs antibodies were found in 146 students (97.3%); anti-HCV in 3 cases (2%), and anti-HAV in 15 cases (10%). After 2-fold vaccination against viral hepatitis A, 100% of the students presented with anti-HAV. CONCLUSIONS: The incidence of HBsAg and anti-HCV among our students is the same as in the general population, while the incidence of anti-HAV is much lower (10%). The presence of anti-HBs 4 years after vaccination was detected in 97.3% of the students, and anti-HAV 1 month after the complete course of vaccination in 100% of cases.


Subject(s)
Hepatitis A Vaccines/immunology , Hepatitis A/prevention & control , Hepatitis B Vaccines/immunology , Hepatitis B/prevention & control , Hepatitis Viruses/metabolism , Adult , Antibody Formation , Female , Hepatitis A Vaccines/therapeutic use , Hepatitis B Surface Antigens/analysis , Hepatitis B Vaccines/therapeutic use , Humans , Male , Students, Medical , Time Factors
15.
Virology ; 202(2): 1061-4, 1994 Aug 01.
Article in English | MEDLINE | ID: mdl-8030212

ABSTRACT

We have previously identified a 180-kDa host cell glycoprotein (gp180) that specifically binds the surface envelope of duck hepatitis B virus (DHBV) and whose binding is inhibited by neutralizing antiviral monoclonal antibodies. Here we map the viral determinants required for gp180 binding to a 66-amino acid region within the preS domain of the envelope coding region. This region includes both major neutralizing preS epitopes previously defined by monoclonal antibodies. Examination of a series of linker-substitution mutations throughout preS indicates that all mutations that block gp180 binding ablate virus infectivity. Interestingly, two mutations that do not prevent binding can also impair infectivity.


Subject(s)
Hepatitis Virus, Duck/metabolism , Membrane Glycoproteins/metabolism , Receptors, Virus/metabolism , Viral Envelope Proteins/metabolism , Amino Acid Sequence , Animals , Binding Sites , Ducks/microbiology , Hepatitis B Surface Antigens/chemistry , Hepatitis Viruses/metabolism , Molecular Sequence Data , Protein Binding , Recombinant Fusion Proteins , Structure-Activity Relationship
16.
J Virol ; 66(6): 3339-46, 1992 Jun.
Article in English | MEDLINE | ID: mdl-1583719

ABSTRACT

Previously, we established a system whereby an intergenic region from mouse hepatitis virus (MHV) inserted into an MHV defective interfering (DI) RNA led to transcription of a subgenomic DI RNA in helper virus-infected cells. By using this system, the duration of a primary transcription initiation activity which transcribes subgenomic-size RNAs from the genomic-size RNA template in MHV-infected cells was examined. Efficient DI genomic and subgenomic RNA synthesis was observed when the DI RNA was transfected at 1, 3, 3.5, 5, and 6 h postinfection, indicating that all activities which are necessary for MHV RNA synthesis are present continuously during the first 6 h of infection. The effect of subgenomic DI RNA synthesis on DI genomic RNA replication was then examined. Replication efficiency of the DI genomic RNA which synthesized the subgenomic RNA was approximately 70% lower than that of DI genomic RNA which did not synthesize the subgenomic DI RNA in MHV-infected cells. Cotransfection of two different-size DI RNAs demonstrated that replication of the larger DI RNA was strongly inhibited by replication of the smaller genomic DI RNA. Cotransfection of two DI RNA species of the same length into MHV-infected cells demonstrated that reduced replication of the genomic DI RNA which synthesizes the subgenomic RNA did not affect the replication of cotransfected DI RNA, demonstrating that the reduction in DI genomic RNA replication works only in cis, not in trans. Therefore, the previously proposed hypothesis that coronavirus, subgenomic RNA synthesis may inhibit the replication of genomic RNA by competing for a limited amount of virus-derived factors seems unlikely. Possible mechanisms of coronavirus transcription are discussed.


Subject(s)
Coronaviridae/metabolism , Hepatitis Viruses/metabolism , Transcription, Genetic , Animals , Cells, Cultured , Gene Expression Regulation, Viral , Genome, Viral , Helper Viruses/metabolism , Mice , Models, Genetic , Mutagenesis , RNA, Viral/biosynthesis , Time Factors , Transfection , Virus Replication
17.
New Biol ; 3(6): 531-7, 1991 Jun.
Article in English | MEDLINE | ID: mdl-1680384

ABSTRACT

The primary transcripts encoded by retroelements contain two polyadenylation [poly(A)] sites, positioned 5' and 3' of the protein-coding sequences. To ensure efficient gene expression in these systems, a mechanism must operate that suppresses use of the 5' site, enhances use of the 3' site, or both. These possibilities have been examined in a recent series of experiments that have revealed two main regulatory themes. First, maximum 3' end processing can require sequences other than core poly(A) signals, which are transcribed only before the 3' site. Second, processing is partly inhibited when the site is positioned close to the promoter. These results should serve as the basis for a more detailed understanding of how the choice of the poly(A) site is regulated in these elements.


Subject(s)
Poly A/genetics , RNA, Messenger/genetics , RNA, Viral/genetics , Base Sequence , Gene Expression , HIV-1/genetics , HIV-1/metabolism , Hepatitis Viruses/genetics , Hepatitis Viruses/metabolism , Mosaic Viruses/genetics , Mosaic Viruses/metabolism , Poly A/metabolism , Promoter Regions, Genetic , RNA Processing, Post-Transcriptional , RNA, Messenger/metabolism , RNA, Viral/metabolism , Retroviridae/genetics , Retroviridae/metabolism , Transcription, Genetic
18.
J Virol ; 60(3): 943-9, 1986 Dec.
Article in English | MEDLINE | ID: mdl-3783821

ABSTRACT

Binding sites for polymerized albumin on hepatitis B virus components were reported in human hepatitis B virus chronic carriers predominantly with active viral replication (HB e antigen positive). The presence of comparable albumin-binding sites in the woodchuck hepatitis virus (WHV) model was examined on WHV components obtained from woodchucks with active viral replication (DNA polymerase positive). Binding sites for polymerized woodchuck serum albumin were not detected on the intact WHV virion, on 22-nm woodchuck hepatitis surface antigen (WHsAg), or on WHsAg polypeptides. Woodchuck albumin was not detected in purified 22-nm WHsAg, and anti-albumin antibodies were not detected in WHV chronic-carrier woodchucks. Our results in the WHV model argue against a role for viral polyalbumin-binding sites in tissue- and host-specific virus infectivity.


Subject(s)
Antigens, Viral/metabolism , Hepatitis B/etiology , Hepatitis Viruses/immunology , Serum Albumin/metabolism , Animals , Antigens, Surface/metabolism , Binding Sites , Hepatitis Viruses/metabolism , Humans , Marmota/microbiology , Protein Binding , Radioimmunoassay , Serum Albumin, Human , Viral Proteins/metabolism , Virion/immunology
19.
J Infect Dis ; 147(3): 535-9, 1983 Mar.
Article in English | MEDLINE | ID: mdl-6403632

ABSTRACT

Fecal and serum samples from a chimpanzee with acute-phase, blood-borne non-A, non-B hepatitis were administered to four chimpanzees. Fecal material given either intravenously or orally did not result in abnormal levels of alanine aminotransferase or the occurrence of ultrastructural alterations in hepatocellular cytoplasm characteristic of non-A, non-B hepatitis during a one-year period of follow-up. Definite non-A, non-B hepatitis was later demonstrated in two of these animals four weeks after inoculation of acute-phase serum. Thus, feces do not appear to transmit this human strain of blood-borne non-A, non-B hepatitis.


Subject(s)
Feces/microbiology , Hepatitis C/transmission , Hepatitis, Viral, Human/transmission , Animals , Cytoskeleton/ultrastructure , Female , Hepatitis C/microbiology , Hepatitis Viruses/metabolism , Humans , Liver/ultrastructure , Microscopy, Electron
20.
Biochim Biophys Acta ; 640(1): 91-9, 1981 Jan 08.
Article in English | MEDLINE | ID: mdl-6260173

ABSTRACT

A sulfonium analog of choline ('sulfocholine', a natural phospholipid constituent of diatoms) was metabolically incorporated into mouse LM fibroblasts cultured in serum-free medium. Subconfluent cultures of LM cells were able to utilize sulfocholine as sole choline source and to increase in cell number for 3 days of incubation; thereafter a decrease in cell number was observed. In contrast, cultures of LM cells seeded to confluency showed no decrease in cell number up to at least 10 days when maintained, with daily medium changes, in medium containing either choline or the sulfonium analog. Such confluent cultures, maintained for 7 days in sulfocholine-containing medium, showed virtually complete replacement of cellular phosphatidylcholine and greater than 50% replacement of cellular sphingomyelin by their respective sulfonium analogs. The functional exchangeability of natural phosphatidylcholine and sphingomyelin with their sulfonium analogs to participate in normal cell membrane-mediated activities was demonstrated by comparatively assaying the abilities of sulfocholine- and choline-maintained cells to incorporate and replicate certain animal viruses known to possess membrane-dependent steps in various phases of their replication cycles. No difference was detected between the abilities of sulfocholine- and choline-maintained cells to take up vesicular stomatitis virus or mengo virus, or to replicate vesicular stomatitis virus, mengo virus or mouse hepatitis virus.


Subject(s)
Cell Membrane/physiology , Cell Transformation, Viral/drug effects , Choline/analogs & derivatives , Membrane Lipids/physiology , Phospholipids/physiology , Animals , Cell Division , Choline/metabolism , Choline/pharmacology , Choline/physiology , Hepatitis Viruses/metabolism , Kinetics , L Cells/physiology , Mengovirus/metabolism , Mice , Vesicular stomatitis Indiana virus/metabolism , Virus Replication/drug effects
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