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Bioanalysis
; 7(16): 2105-17, 2015.
Article
in English
| MEDLINE
| ID: mdl-26327189
ABSTRACT
BACKGROUND: Imatinib (IM) is widely used in treatment of chronic myeloid leukemia with target trough plasma concentrations above 1000 ng ml(-1). DBS can increase access to IM therapeutic drug monitoring. RESULTS: IM was measured in the range 50-4000 ng ml(-1) by UHPLC-MS/MS using one 6 mm DBS in a fully validated method. IM was stable at DBS maintained at 40°C for 36 days. Plasma and DBS concentrations were highly correlated (r > 0.96). The use of a IM concentration target of 765 ng ml(-1) in DBS identified 93% of patients with plasma concentration below 1000 ng ml(-1). CONCLUSION: IM can be measured in DBS using UHPLC-MS/MS with results comparable to those obtained in blood plasma.