ABSTRACT
This study explores the food transport efficiency (E) in a termite tunnel consisting of a main tunnel and a 2-segment loop tunnel through a model simulation. Simulated termites navigate between the main and loop tunnels through branching nodes (a, b, c, d) with associated probabilities (P1, P2, P3, P4). The loop tunnel locations (δ) are considered: near the nest (δâ =â 1), at the center of the main tunnel (δâ =â 2), and close to the food site (δâ =â 3). The results reveal that for δâ =â 1, paths such as a â d â b â c and c â d â b â a exhibited high E values. Conversely, for δâ =â 2, P3 and P4 demonstrate elevated E values ranging from 0.4 to 0.6. For δâ =â 3, paths like c â d or c â b display high E values, emphasizing the significance of in-loop separation tunnels (characterized by P3 and P4) in alleviating traffic congestion. Partial rank correlation validates that P1 and P2 minimally influence E, while P3 and P4 significantly negatively impact E, regardless of δ. However, for δâ =â 2, the influence of P3 and P4 is notably reduced due to the positional symmetry of the loop tunnel. In the discussion, we address model limitations and propose strategies to overcome them. Additionally, we outline potential experimental validations to ensure a comprehensive understanding of the dynamics governing termite food transport within tunnels.
Subject(s)
Feeding Behavior , Isoptera , Animals , Isoptera/physiology , Computer Simulation , Models, BiologicalABSTRACT
Methane is a powerful greenhouse gas, more potent than carbon dioxide, and emitted from a variety of natural sources including wetlands, permafrost, mammalian guts and termites. As increases in global temperatures continue to break records, quantifying the magnitudes of key methane sources has never been more pertinent. Over the last 40 years, the contribution of termites to the global methane budget has been subject to much debate. The most recent estimates of termite emissions range between 9 and 15 Tg CH4 year-1, approximately 4% of emissions from natural sources (excluding wetlands). However, we argue that the current approach for estimating termite contributions to the global methane budget is flawed. Key parameters, namely termite methane emissions from soil, deadwood, living tree stems, epigeal mounds and arboreal nests, are largely ignored in global estimates. This omission occurs because data are lacking and research objectives, crucially, neglect variation in termite ecology. Furthermore, inconsistencies in data collection methods prohibit the pooling of data required to compute global estimates. Here, we summarise the advances made over the last 40 years and illustrate how different aspects of termite ecology can influence the termite contribution to global methane emissions. Additionally, we highlight technological advances that may help researchers investigate termite methane emissions on a larger scale. Finally, we consider dynamic feedback mechanisms of climate warming and land-use change on termite methane emissions. We conclude that ultimately the global contribution of termites to atmospheric methane remains unknown and thus present an alternative framework for estimating their emissions. To significantly improve estimates, we outline outstanding questions to guide future research efforts.
Subject(s)
Isoptera , Methane , Isoptera/physiology , Isoptera/metabolism , Methane/analysis , Methane/metabolism , Animals , Climate Change , Greenhouse Gases/analysisABSTRACT
Sociality underpins major evolutionary transitions and significantly influences the structure and function of complex ecosystems. Social insects, seen as the pinnacle of sociality, have traits like obligate sterility that are considered 'master traits', used as single phenotypic measures of this complexity. However, evidence is mounting that completely aligning both phenotypic and evolutionary social complexity, and having obligate sterility central to both, is erroneous. We hypothesize that obligate and functional sterility are insufficient in explaining the diversity of phenotypic social complexity in social insects. To test this, we explore the relative importance of these sterility traits in an understudied but diverse taxon: the termites. We compile the largest termite social complexity dataset to date, using specimen and literature data. We find that although functional and obligate sterility explain a significant proportion of variance, neither trait is an adequate singular proxy for the phenotypic social complexity of termites. Further, we show both traits have only a weak association with the other social complexity traits within termites. These findings have ramifications for our general comprehension of the frameworks of phenotypic and evolutionary social complexity, and their relationship with sterility.
Subject(s)
Isoptera , Social Behavior , Isoptera/physiology , Animals , Biological Evolution , Phenotype , Behavior, AnimalABSTRACT
In social insect colonies, selfish behaviour due to intracolonial conflict among members can result in colony-level costs despite close relatedness. In certain termite species, queens use asexual reproduction for within-colony queen succession but rely on sexual reproduction for worker and alate production, resulting in multiple half-clones of a single primary queen competing for personal reproduction. Our study demonstrates that competition over asexual queen succession among different clone types leads to the overproduction of parthenogenetic offspring, resulting in the production of dysfunctional parthenogenetic alates. By genotyping the queens of 23 field colonies of Reticulitermes speratus, we found that clone variation in the queen population reduces as colonies develop. Field sampling of alates and primary reproductives of incipient colonies showed that overproduced parthenogenetic offspring develop into alates that have significantly smaller body sizes and much lower survivorship than sexually produced alates. Our results indicate that while the production of earlier and more parthenogenetic eggs is advantageous for winning the competition for personal reproduction, it comes at a great cost to the colony. Thus, this study highlights the evolutionary interplay between individual-level and colony-level selection on parthenogenesis by queens.
Subject(s)
Isoptera , Parthenogenesis , Animals , Isoptera/physiology , Isoptera/genetics , Female , Reproduction , Social BehaviorABSTRACT
Termites are social insects with high species diversity in tropical ecosystems. Multivariate analysis with near-infrared spectroscopy (NIRS) and data interpretation can separate social insects belonging to different colonies of the same species. The objective of this study was to propose the use of discriminant analysis by partial least squares (PLS-DA) combined with NIRS to identify the colonial origin of the Syntermes grandis (Rambur, 1842) (Blattodea: Termitidae) in 2 castes. Six ground S. grandis colonies were identified and mapped; 30 workers and 30 soldier termites in each colony were submitted to spectral measurement with NIRS. PLS-DA applied to the termites' spectral absorbance was used to detect a spectral pattern per S. grandis colony by caste. PLS-DA regression with NIRS proved to be an approach with 99.9% accuracy for identifying the colonial origin of S. grandis workers and 98.3% for soldiers. The methodology showed the importance of qualitatively characterizing the colonial phenotypic response of this species. NIRS is a high-precision approach to identifying the colony origin of S. grandis workers and soldiers. The PLS-DA can be used to design ecological field studies to identify colony territorial competition and foraging behavior of subterranean termite species.
Subject(s)
Isoptera , Spectroscopy, Near-Infrared , Isoptera/physiology , Animals , Discriminant Analysis , Least-Squares Analysis , Social BehaviorABSTRACT
Eastern subterranean termites, Reticulitermes flavipes (Kollar), are widely distributed across North America where they are exposed to a broad range of environmental conditions. However, mechanisms for overwintering are not well understood. Wisconsin is a unique location to study mechanisms of cold tolerance as it represents the northern boundary for persistent R. flavipes populations. In this study, we evaluated seasonal shifts in cold tolerance using critical thermal minimum (CTmin) and supercooling point (SCP) and examined how these measurements correlate to changes in the microbial community of the termite gut. Results showed seasonal acclimatization to cold, which is consistent with the use of behavioral freeze-avoidant mechanisms. However, these insects also demonstrated an increased susceptibility to freezing later in the season, which may be tied to changes in gut microbiota. Our results found shifts in the composition of the gut microbiome in R. flavipes between mid- to late summer and early to late fall. These differences may be suggestive of a change in metabolism to adjust to a period of reduced feeding and increased metabolic stress during overwintering. Specifically, results showed an increased abundance of Methanobrevibacter sp. (Euryarchaeota) associated with cold, which may be indicative of a metabolic shift from acetogenesis to methanogenesis associated with overwintering. Further work is needed focusing on specific contributions of certain gut microbes, particularly their role in metabolic adaptability and in providing protection from oxidative stress associated with changes in environmental conditions.
Subject(s)
Acclimatization , Cold Temperature , Gastrointestinal Microbiome , Isoptera , Seasons , Animals , Isoptera/physiology , Isoptera/microbiology , WisconsinABSTRACT
Domains as functional protein units and their rearrangements along the phylogeny can shed light on the functional changes of proteomes associated with the evolution of complex traits like eusociality. This complex trait is associated with sterile soldiers and workers, and long-lived, highly fecund reproductives. Unlike in Hymenoptera (ants, bees, and wasps), the evolution of eusociality within Blattodea, where termites evolved from within cockroaches, was accompanied by a reduction in proteome size, raising the question of whether functional novelty was achieved with existing rather than novel proteins. To address this, we investigated the role of domain rearrangements during the evolution of termite eusociality. Analysing domain rearrangements in the proteomes of three solitary cockroaches and five eusocial termites, we inferred more than 5,000 rearrangements over the phylogeny of Blattodea. The 90 novel domain arrangements that emerged at the origin of termites were enriched for several functions related to longevity, such as protein homeostasis, DNA repair, mitochondrial activity, and nutrient sensing. Many domain rearrangements were related to changes in developmental pathways, important for the emergence of novel castes. Along with the elaboration of social complexity, including permanently sterile workers and larger, foraging colonies, we found 110 further domain arrangements with functions related to protein glycosylation and ion transport. We found an enrichment of caste-biased expression and splicing within rearranged genes, highlighting their importance for the evolution of castes. Furthermore, we found increased levels of DNA methylation among rearranged compared to non-rearranged genes suggesting fundamental differences in their regulation. Our findings indicate the importance of domain rearrangements in the generation of functional novelty necessary for termite eusociality to evolve.
Subject(s)
Biological Evolution , Isoptera , Animals , Isoptera/genetics , Isoptera/physiology , Social Behavior , Phylogeny , Proteome/genetics , Insect Proteins/genetics , Insect Proteins/metabolism , Protein Domains , Blattellidae/geneticsABSTRACT
The white-bellied pangolin Phataginus tricuspis (Rafinesque 1821) is a semiarboreal species occurring in tropical sub-Saharan Africa. It is the world's most trafficked African pangolin species based on volumes recorded in seizures. Reintroduction of confiscated live pangolins and ex-situ rearing are being explored worldwide as a conservation action. However, the husbandry of seized animals is challenging as the diet of the white-bellied pangolin is poorly known and little studied. We analyzed the stomach contents of dead white-bellied pangolins from two forest-savanna protected areas. Stomach content samples from 13 white-bellied pangolin specimens contained ~165,000 Arthropoda, mostly Hymenoptera (60.34%) and Blattodea (39.66%). Overall, we identified 39 termite and 105 ant species consumed as prey by pangolins. Individual pangolins examined had fed on a maximum of 31 ant species and 13 termite species. The termite and ant species richness varied significantly across the pangolins' last consumed meal. We recorded 24 ant genera dominated by Crematogaster (relative importance [RI] = 17.28). Out of 18 termite genera recorded, the genus Pseudacanthotermes (RI = 17.21) was the most important prey. Ten ant species were preferentially eaten by white-bellied pangolin, with Crematogaster acis being the most common prey species. Four species of termite were most frequently eaten with Pseudacanthotermes militaris being the most abundant. The mean abundance of ants and termites varied among pangolin individuals. The season did not influence the mean abundance of termites eaten by pangolin individuals. However, ant abundance in stomach contents was significantly higher in the dry season. An improved understanding of pangolin feeding behavior and prey selection may help inform conservation husbandry efforts. For example, nutritional analysis of the food eaten by wild pangolins can guide the development of nutritional diets for captive pangolins.
Subject(s)
Ants , Diet , Isoptera , Pangolins , Animals , Cameroon , Ants/physiology , Isoptera/physiology , Diet/veterinary , Pangolins/physiology , Ecosystem , Forests , Gastrointestinal Contents , Feeding Behavior/physiologyABSTRACT
It is generally believed that termites can't learn and are not "intelligent". This study aimed to test whether termites could have any form of memory. A Y-shaped test device with one release chamber and two identical test chambers was designed and constructed by 3D printing. A colony of damp wood termites was harvested from the wild. Worker termites were randomly selected for experiment. Repellent odors that could mimic the alarm pheromone for termites were first identified. Among all substances tested, a tea tree oil and lemon juice were found to contain repellent odors for the tested termites, as they significantly reduced the time that termites spent in the chamber treated with these substances. As control, a trail pheromone was found to be attractive. Subsequently, a second cohort of termites were operant conditioned by punishment using both tea tree oil and lemon juice, and then tested for their ability to remember the path that could lead to the repellant odors. The test device was thoroughly cleaned between trials. It was found that conditioned termites displayed a reduced tendency to choose the path that led to expectant punishment as compared with naïve termites. Thus, it is concluded that damp wood termites are capable of learning and forming "fear memory", indicative of "intelligence" in termites. This result challenges established presumption about termites' intelligence.
Subject(s)
Isoptera , Odorants , Isoptera/physiology , Animals , Conditioning, Operant/physiology , Pheromones/pharmacology , Memory/physiology , Learning/physiology , Tea Tree Oil/pharmacology , Citrus , Insect Repellents/pharmacology , Behavior, Animal/physiology , PunishmentABSTRACT
In social insects, interactions among colony members trigger caste differentiation with morphological modifications. During caste differentiation in termites, body parts and caste-specific morphologies are modified during postembryonic development under endocrine controls such as juvenile hormone (JH) and ecdysone. In addition to endocrine factors, developmental toolkit genes such as Hox- and appendage-patterning genes also contribute to the caste-specific body part modifications. These toolkits are thought to provide spatial information for specific morphogenesis. During social evolution, the complex crosstalks between physiological and developmental mechanisms should be established, leading to the sophisticated caste systems. This article reviews recent studies on these mechanisms underlying the termite caste differentiation and addresses implications for the evolution of caste systems in termites.
Subject(s)
Isoptera , Juvenile Hormones , Animals , Isoptera/genetics , Isoptera/physiology , Isoptera/growth & development , Juvenile Hormones/metabolism , Gene Expression Regulation, Developmental , Ecdysone/metabolismABSTRACT
Hybridization between invasive pest species may lead to significant genetic and economic impacts that require close monitoring. The two most invasive and destructive termite species worldwide, Coptotermes formosanus Shiraki and Coptotermes gestroi (Wasmann), have the potential for hybridization in the field. A three-year field survey conducted during the dispersal flight season of Coptotermes in Taiwan identified alates with atypical morphology, which were confirmed as hybrids of the two Coptotermes species using microsatellite and mitochondrial analyses. Out of 27,601 alates collected over three years, 4.4% were confirmed as hybrid alates, and some advanced hybrids (>F1 generations) were identified. The hybrid alates had a dispersal flight season that overlapped with the two parental species 13 out of 15 times. Most of the hybrid alates were females, implying that mating opportunities beyond F1 may primarily be possible through female hybrids. However, the incipient colony growth results from all potential mating combinations suggest that only backcross colonies with hybrid males could sometimes lead to brood development. The observed asymmetrical viability and fertility of hybrid alates may critically reduce the probability of advanced-hybrid colonies being established in the field.
Subject(s)
Gene Flow , Hybridization, Genetic , Isoptera , Microsatellite Repeats , Animals , Isoptera/genetics , Isoptera/physiology , Female , Male , Microsatellite Repeats/genetics , Taiwan , Introduced Species , DNA, Mitochondrial/geneticsABSTRACT
Termite eusociality is accompanied by flagrant caste polyphenism manifested by the presence of several sterile (workers and soldiers) and reproductive (imaginal and neotenic kings and queens) caste phenotypes. Imaginal kings and queens are developmentally equivalent to adults of other hemimetabolous insects but display multiple adaptations inherent to their role of eusocial colony founders, such as long lifespan and high fecundity. Herein, we summarize the recent advances in understanding the biology of imaginal (primary) queens as emblematic examples of termite polyphenism acquired during social evolution. We focus on the control of queen development, on dynamics in physiology and fecundity during the queen's life, on new findings about queen fertility signaling, and on proximate mechanisms underlying queen longevity.
Subject(s)
Isoptera , Animals , Isoptera/physiology , Fertility , Reproduction , Longevity , PhenotypeABSTRACT
Physogastric termite queens are characterized by a notorious enlargement of the abdomen triggered by an equal development of the ovaries. Other physogastry-related modifications have been reported on the fat body, cuticle, midgut, tracheal system, and hemolymph. Surprisingly, modifications on the lateral oviducts of these females, important sites for ovulation and egg transport, have received little attention. Here we took advantage of the high fecundity of physogastric queens in three termitid species to evaluate ovary development and also to compare the morphophysiological features of the lateral oviducts between early-mated and physogastric queens of Cornitermes cumulans. Older queens show well-developed ovaries, with numerous ovarioles connected to the lateral oviducts through pedicels. At these sites, several corpora lutea were observed, residual follicle cells from previous ovulation events. Such features were absent among early-mated queens and reflect then the maturity and ageing of the queens. Histological and histochemical analyses indicated that secretory activity of the lateral oviducts was also restricted to physogastric queens, in which proteins, but not polysaccharides, are secreted into the oviduct lumen. The likely function of these proteins, based on previous studies, is to lubricate the lateral oviducts and stimulate muscular contractions to the egg transport. The physogastry of termite queens is a notorious feature, characterized by several body modifications, especially concerning the ovaries. Our results shed light on the physogastry-related changes in the lateral oviducts of termite queens, as their increasing secretory activity is in agreement with the high number of eggs produced and transporting through these structures. Thus, such changes correspond to an important step allowing the high egg-laying rate shown by physogastric termite queens.
Subject(s)
Isoptera , Female , Animals , Isoptera/physiology , Reproduction/physiology , Ovary , Ovarian Follicle , OviductsABSTRACT
Biocontrol of subterranean termites is largely impeded by their social immune responses. Studies on biocontrol agents combined with natural insecticides and their possible effects on the immune defense mechanisms of termites are limited. In this study, we investigated the effects of a combined biocontrol strategy using a plant-derived insect ATPase inhibitor, α-terpineol, with the entomopathogenic nematodes (EPNs) Steinernema carpocapsae against the subterranean termite Coptotermes formosanus Shiraki. Survival assays showed that even a low lethal concentration of α-terpineol significantly increased the EPNs-induced virulence in C. formosanus. α-terpineol treatment majorly inhibited the activity of Na+- K+- ATPase, which disturbed the EPNs-induced enhancement of locomotor activity and grooming behavior in termites treated with the combined strategy. Furthermore, the combination treatment had a synergistic inhibitory effect on innate immune responses in C. formosanus, which were measured as changes in the expression of immune-related genes and activities of immune system enzymes. In conclusion, α-terpineol can weaken the immune defense of termites against EPNs at low lethal concentrations, and is a suitable non-synthetic insecticide to prove the biocontrol efficiency of EPNs on C. formosanus. This study provides a theoretical basis and technical reference for a novel biocontrol strategy that promises to overcome the problems of host immune defense in termites.
Subject(s)
Insecticides , Isoptera , Nematoda , Animals , Isoptera/physiology , Virulence , Insecticides/pharmacology , Adenosine TriphosphatasesABSTRACT
The care-kill response determines whether a sick individual will be treated or eliminated from an insect society, but little is known about the physiological underpinnings of this process. We exploited the stepwise infection dynamics of an entomopathogenic fungus in a termite to explore how care-kill transitions occur, and identify the chemical cues behind these shifts. We found collective responses towards pathogen-injected individuals to vary according to severity and timing of pathogen challenge, with elimination, via cannibalism, occurring sooner in response to a severe active infection. However, injection with inactivated fungal blastospores also resulted in increased albeit delayed cannibalism, even though it did not universally cause host death. This indicates that the decision to eliminate an individual is triggered before pathogen viability or terminal disease status has been established. We then compared the surface chemistry of differently challenged individuals, finding increased amounts of long-chained methyl-branched alkanes with similar branching patterns in individuals injected with both dead and viable fungal blastospores, with the latter showing the largest increase. This coincided with the highest amounts of observed cannibalism as well as signs of severe moribundity. Our study provides new mechanistic insight into the emergent collective behaviors involved in the disease defense of a termite society.
Subject(s)
Isoptera , Humans , Animals , Isoptera/physiology , CannibalismABSTRACT
Heuweltjies are earthen mounds found throughout the Succulent Karoo of South Africa and are inhabited by the termite Microhodotermes viator. Many have assumed that heuweltjies are constructed by the occupying termites. Consequently, heuweltjies have been used as an example of several important concepts in ecology and evolution: the extended phenotype, ecosystem engineering and niche construction. However, recent findings demonstrate that M. viator does not directly construct heuweltjies. Rather, termite colonies enrich the soil around their nests with plant nutrients, which promotes development of widely separated patches of denser vegetation. Eventual formation of heuweltjies represents a response of the physical environment to the windbreak effect of the denser vegetation patches (localized reduction of wind velocity and resultant deposition and accumulation of airborne sediment). Other structures constructed by the termites are justifiably regarded as extended phenotypes. Identification and investigation of a complex cascade of processes are required to more precisely assess the manner in which this termite species functions as an ecosystem engineer or niche constructor, thereby significantly influencing the availability of resources within local ecosystems. Environmental alterations that are either directly or indirectly generated by social animals that construct large, communal nests represent ecological processes that contribute significantly to local biodiversity. This article is part of the theme issue 'The evolutionary ecology of nests: a cross-taxon approach'.
Subject(s)
Ecosystem , Isoptera , Animals , Isoptera/physiology , South Africa , Biodiversity , PhenotypeABSTRACT
The family Termitidae is renowned for its diverse nesting behaviors, with the evolution of epigeal and arboreal nests hypothesized to increase desiccation stress due to greater exposure to air. However, these nests may also alleviate desiccation stress through humidity regulation. To explore the implications of acquiring epigeal and arboreal nests, we investigated desiccation tolerance traits in 16 Termitidae termite species with varying nest types and analyzed trait correlations. Principal component analysis revealed that termites constructing epigeal and arboreal nests exhibited reduced water loss rates and enhanced survival under desiccated conditions. Furthermore, termites building arboreal nests displayed a notably higher water content. Redundancy analysis demonstrated that nest types accounted for a substantial portion (57.2%) of the observed variation in desiccation tolerance. These findings support the hypothesis that epigeal and arboreal nests in termites are associated with increased desiccation stress and increased desiccation tolerance. These findings highlight the role of nest type in influencing desiccation tolerance mechanisms and water regulation strategies in termites.
Subject(s)
Isoptera , Animals , Isoptera/physiology , Desiccation , Water , Nesting Behavior , HumidityABSTRACT
This study explores the food transport efficiency of termite using an individual-based model. Termites are believed to have evolved tunneling patterns that optimize food search and transport efficiency. However, few studies have investigated transport efficiency due to the difficulty of field observations. The model is characterized by four control variables: the number of simulated termites participating in transport (k1), the distribution of high curvature sections of the termite tunnel (k2), a quantity related to the density of the tunnel sections (k3), and the duration of traffic jams (k4). As k3 increases, the total length of the high curvature section decreases. Our simulation results show that the E(k1, k2) maps for k3 and k4 contain two modes: Mode A shows that E decreases with increasing k1 due to an increase in traffic jams, while Mode B shows E increasing with increasing k1 due to a decrease in the density of curved sections and an increase in jamming resolution time. The partial rank correlation coefficient analysis reveals that k1 and k2 have a negative effect on E, while k3 and k4 have a positive effect, with k1 having the greatest influence on E, followed by k3, k4, and k2. The ecological implications of the simulation results are briefly described, and the limitations of the model are discussed.
Subject(s)
Appetitive Behavior , Isoptera , Animals , Isoptera/physiology , Models, BiologicalABSTRACT
Division of labor is a prominent feature of social insect societies, where different castes engage in different specialized tasks. As brain differences are associated with behavioral differences, brain anatomy may be linked to caste polymorphism. Here, we show that termite brain morphology changes markedly with caste differentiation and age in the termite, Reticulitermes speratus. Brain morphology was shown to be associated with reproductive division of labor, with reproductive individuals (alates and neotenic reproductives) having larger brains than nonreproductives (workers and soldiers). Micro-computed tomography (CT) imaging and dissection observations showed that the king's brain morphology changed markedly with shrinkage of the optic lobes during their long life in the dark. Behavioral experiments showed that mature primary kings lose visual function as a result of optic lobe shrinkage. These results suggested that termites restructure their nervous systems to perform necessary tasks as they undergo caste differentiation, and that they also show flexible changes in brain morphology even after the final molt. This study showed that brain morphology in social insects is linked to caste and aging, and that the evolution of the division of labor is underpinned by the development of diverse neural systems for specialized tasks.
Subject(s)
Isoptera , Humans , Animals , Isoptera/physiology , X-Ray Microtomography , Aging , Brain/diagnostic imagingABSTRACT
Soil moisture is a critical environmental factor for the survival and behavior of subterranean termites (family Rhinotermitidae). The invasive Formosan subterranean termite, Coptotermes formosanus Shiraki, and the native eastern subterranean termite, Reticulitermes flavipes (Kollar), co-occur in the southeastern United States, while R. flavipes is distributed in a wider geoclimatic range. Previous studies showed that subterranean termites preferred higher soil moisture levels for tunneling and feeding; however, the impacts of constant moisture remained to be characterized to understand their moisture tolerance. In this study, we hypothesized that different soil moisture regimes can alter termite foraging and survival, and that the effects differ between the two species. The tunneling activity, survivorship, and food consumption of termites were documented for 28 days with different sand moisture conditions ranging from no moisture to full saturation (0%, 1%, 5%, 15%, 25%, and 30%). We found that there were no significant differences in the responses between C. formosanus and R. flavipes. In both species, termites did not survive or tunnel with 0% moisture. Termites performed tunneling with only 1% sand moisture, although they did not survive for 28 days. A minimal of 5% sand moisture was required for survival, and there were no significant differences in survivorship, tunneling activity, or food consumption among moisture contents of 5-30%. The results suggest that subterranean termites are resilient to moisture extremes. Colonies can tolerate low moisture conditions in their foraging environment for extended times, which may allow them to tunnel and find new moisture sources for colony survival.