ABSTRACT
Peptidyl nucleoside antibiotics (PNAs) are a diverse class of natural products with promising biomedical activities. These compounds have tripartite structures composed of a core saccharide, a nucleobase, and one or more amino acids. In particular, amipurimycin and the miharamycins are novel 2-aminopurinyl PNAs with complex nine-carbon core saccharides and include the unusual amino acids (-)-cispentacin and N5-hydroxyarginine, respectively. Despite their interesting structures and properties, these PNAs have heretofore eluded biochemical scrutiny. Herein is reported the discovery and initial characterization of the miharamycin gene cluster in Streptomyces miharaensis (mhr) and the amipurimycin gene cluster (amc) in Streptomyces novoguineensis and Streptomyces sp. SN-C1. The gene clusters were identified using a comparative genomics approach, and heterologous expression of the amc cluster as well as gene interruption experiments in the mhr cluster support their role in the biosynthesis of amipurimycin and the miharamycins, respectively. The mhr and amc biosynthetic gene clusters characterized encode enzymes typical of polyketide biosynthesis instead of enzymes commonly associated with PNA biosynthesis, which, along with labeled precursor feeding studies, implies that the core saccharides found in the miharamycins and amipurimycin are partially assembled as polyketides rather than derived solely from carbohydrates. Furthermore, in vitro analysis of Mhr20 and Amc18 established their roles as ATP-grasp ligases involved in the attachment of the pendant amino acids found in these PNAs, and Mhr24 was found to be an unusual hydroxylase involved in the biosynthesis of N5-hydroxyarginine. Finally, analysis of the amc cluster and feeding studies also led to the proposal of a biosynthetic pathway for (-)-cispentacin.
Subject(s)
Anti-Bacterial Agents/biosynthesis , N-Glycosyl Hydrolases/biosynthesis , Nucleosides/biosynthesis , Purines/biosynthesis , Anti-Bacterial Agents/chemistry , Biosynthetic Pathways , Molecular Conformation , Multigene Family , N-Glycosyl Hydrolases/chemistry , N-Glycosyl Hydrolases/genetics , Nucleosides/chemistry , Nucleosides/genetics , Purines/chemistry , Streptomyces/geneticsABSTRACT
BACKGROUND: Huntington's disease (HD) is a devastating neurodegenerative disorder caused by CAG triplet expansions in the huntingtin gene. Oxidative stress is linked to HD pathology, although it is not clear whether this is an effect or a mediator of disease. The transgenic (TgHD) minipig expresses the N-terminal part of human-mutated huntingtin and represents a unique model to investigate therapeutic strategies towards HD. A more detailed characterization of this model is needed to fully utilize its potential. METHODS: In this study, we focused on the molecular and cellular features of fibroblasts isolated from TgHD minipigs and the wild-type (WT) siblings at different ages, pre-symptomatic at the age of 24-36 months and with the onset of behavioural symptoms at the age of 48 months. We measured oxidative stress, the expression of oxidative stress-related genes, proliferation capacity along with the expression of cyclin B1 and D1 proteins, cellular permeability, and the integrity of the nuclear DNA (nDNA) and mitochondrial DNA in these cells. RESULTS: TgHD fibroblasts isolated from 48-month-old animals showed increased oxidative stress, which correlated with the overexpression of SOD2 encoding mitochondrial superoxide dismutase 2, and the NEIL3 gene encoding DNA glycosylase involved in replication-associated repair of oxidized DNA. TgHD cells displayed an abnormal proliferation capacity and permeability. We further demonstrated increased nDNA damage in pre-symptomatic TgHD fibroblasts (isolated from animals aged 24-36 months). CONCLUSIONS: Our results unravel phenotypic alterations in primary fibroblasts isolated from the TgHD minipig model at the age of 48 months. Importantly, nDNA damage appears to precede these phenotypic alterations. Our results highlight the impact of fibroblasts from TgHD minipigs in studying the molecular mechanisms of HD pathophysiology that gradually occur with age.
Subject(s)
Aging/metabolism , Fibroblasts/metabolism , Huntingtin Protein/metabolism , Animals , Animals, Genetically Modified , Cell Division , DNA Damage , DNA, Mitochondrial/genetics , Gene Expression Regulation , Humans , Huntingtin Protein/genetics , Lipid Peroxidation , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , Oxidative Stress , Phenotype , Primary Cell Culture , Reactive Oxygen Species/metabolism , Superoxide Dismutase/biosynthesis , Superoxide Dismutase/genetics , Swine , Swine, MiniatureABSTRACT
Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of Escherichia coli can be enhanced by overexpressing ybeD, which was known to encode a hypothetical protein of unknown function. In the wild-type E. coli BL21(DE3), ybeD transcription level increased over five-fold when temperature was increased from 37°C to either 42°C or 46°C. To study the function of ybeD, a deletion strain and an overexpression strain were constructed. At 46°C, in comparison to the wild type, the ybeD-deletion reduced cell growth half-fold, and the ybeD-overexpression promoted cell growth over two-fold. The growth enhancement by ybeD-overexpression was much more pronounced at 46°C than 37°C. The ybeD-overexpression was also effective in other E. coli strains of MG1655, W3110, DH10B, and BW25113. These findings reveal that ybeD gene plays an important role in enduring high-temperature stress, and that ybeD-overexpression can be a prospective strategy to develop thermotolerant microbial hosts.
Subject(s)
Escherichia coli Proteins/biosynthesis , Escherichia coli Proteins/genetics , Escherichia coli/genetics , Escherichia coli/metabolism , Gene Expression Regulation, Bacterial , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , Thermotolerance/genetics , Cell Count , DNA, Bacterial/analysis , Escherichia coli/growth & development , Genes, Bacterial/genetics , Heat-Shock Proteins/genetics , Heat-Shock Proteins/metabolism , Hot Temperature , Sequence Deletion , Thermotolerance/physiologyABSTRACT
The nucleoside hydrolase gene from Leishmania donovani was cloned and expressed in Escherichia coli as a full length 36-kDa protein (LdNH36). Following lysis and extraction, the protein was purified by anion exchange and gel filtration chromatography. The purified protein had a molecular mass of approximately 36-kDa and was confirmed to be >99% pure. Using a nucleoside hydrolase assay, the protein was found to exhibit a Km of 741 ± 246 µM. Protein integrity was confirmed by lithium dodecyl sulfate polyacrylamide gel electrophoresis (LDS-PAGE), mass spectrometry (MS), and enzymatic assay. Analysis of antibody levels from immunized mice indicated that LdNH36 alone or in a stable emulsion with the Toll-like receptor-4 ligand glucopyranosyl lipid adjuvant (GLA-SE) as immunostimulant induced high levels of antigen-specific IgG antibodies. The cellular immune response indicated a Th1 response in mice immunized with LdNH36, but only when formulated with GLA-SE. Mice immunized with the LdNH36 antigen in combination with the GLA-SE adjuvant and challenged with Leishmania mexicana showed significant reductions (>20 fold) in parasite burden, confirming the protective efficacy of this vaccine candidate.
Subject(s)
Immunogenicity, Vaccine , Leishmania donovani , Leishmaniasis Vaccines , Leishmaniasis, Cutaneous , N-Glycosyl Hydrolases , Protozoan Proteins , Animals , Female , Leishmania donovani/enzymology , Leishmania donovani/genetics , Leishmania donovani/immunology , Leishmaniasis Vaccines/biosynthesis , Leishmaniasis Vaccines/immunology , Leishmaniasis Vaccines/isolation & purification , Leishmaniasis Vaccines/pharmacokinetics , Leishmaniasis, Cutaneous/immunology , Leishmaniasis, Cutaneous/prevention & control , Mice , Mice, Inbred BALB C , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/immunology , N-Glycosyl Hydrolases/isolation & purification , N-Glycosyl Hydrolases/pharmacology , Protozoan Proteins/biosynthesis , Protozoan Proteins/immunology , Protozoan Proteins/isolation & purification , Protozoan Proteins/pharmacology , Recombinant Proteins/biosynthesis , Recombinant Proteins/immunology , Recombinant Proteins/isolation & purification , Recombinant Proteins/pharmacologyABSTRACT
Adprhl1, a member of the ADP-ribosylhydrolase protein family, is expressed exclusively in the developing heart of all vertebrates. In the amphibian Xenopus laevis, distribution of its mRNA is biased towards actively growing chamber myocardium. Morpholino oligonucleotide-mediated knockdown of all Adprhl1 variants inhibits striated myofibril assembly and prevents outgrowth of the ventricle. The resulting ventricles retain normal electrical conduction and express markers of chamber muscle differentiation but are functionally inert. Using a cardiac-specific Gal4 binary expression system, we show that the abundance of Adprhl1 protein in tadpole hearts is tightly controlled through a negative regulatory mechanism targeting the 5'-coding sequence of Xenopus adprhl1. Over-expression of full length (40kDa) Adprhl1 variants modified to escape such repression, also disrupts cardiac myofibrillogenesis. Disarrayed myofibrils persist that show extensive branching, with sarcomere division occurring at the actin-Z-disc boundary. Ultimately, Adprhl1-positive cells contain thin actin threads, connected to numerous circular branch points. Recombinant Adprhl1 can localize to stripes adjacent to the Z-disc, suggesting a direct role for Adprhl1 in modifying Z-disc and actin dynamics as heart chambers grow. Modelling the structure of Adprhl1 suggests this cardiac-specific protein is a pseudoenzyme, lacking key residues necessary for ADP-ribosylhydrolase catalytic activity.
Subject(s)
Actin Cytoskeleton/physiology , Gene Expression Regulation, Developmental , Myocardium/cytology , N-Glycosyl Hydrolases/physiology , Xenopus Proteins/physiology , Animals , Animals, Genetically Modified , Gene Knockdown Techniques , Heart/embryology , Heart/growth & development , Heart Ventricles/embryology , Heart Ventricles/growth & development , Humans , Larva , Luminescent Proteins/analysis , Luminescent Proteins/genetics , Mice , Models, Molecular , Molecular Dynamics Simulation , Morpholinos/pharmacology , Mutation , Myocardium/metabolism , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , Organogenesis , Protein Conformation , RNA, Messenger/biosynthesis , Recombinant Fusion Proteins/metabolism , Xenopus Proteins/biosynthesis , Xenopus Proteins/genetics , Xenopus laevis/embryology , Xenopus laevis/growth & developmentABSTRACT
Endonuclease VIII-like 3 (Neil3) is one of the five DNA glycosylases found in mammals that recognize and remove oxidized bases, and initiate the base excision repair (BER) pathway. Previous attempts to express and purify the mouse and human orthologs of Neil3 in their active form have not been successful. Here we report the construction of bicistronic expression vectors for expressing in Escherichia coli the full-length mouse Neil3 (MmuNeil3), its glycosylase domain (MmuNeil3Δ324), as well as the glycosylase domain of human Neil3 (NEIL3Δ324). The purified Neil3 proteins are all active, and NEIL3Δ324 exhibits similar glycosylase/lyase activity as MmuNeil3Δ324 on both single-stranded and double-stranded substrates containing thymine glycol (Tg), spiroiminodihydantoin (Sp) or an abasic site (AP). We show that N-terminal initiator methionine processing is critical for the activity of both mouse and human Neil3 proteins. Co-expressing an E. coli methionine aminopeptidase (EcoMap) Y168A variant with MmuNeil3, MmuNeil3Δ324 and NEIL3Δ324 improves the N-terminal methionine processing and increases the percentage of active Neil3 proteins in the preparation. The purified Neil3 proteins are suitable for biochemical, structural and functional studies.
Subject(s)
Endodeoxyribonucleases/biosynthesis , Endodeoxyribonucleases/isolation & purification , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/isolation & purification , Recombinant Fusion Proteins/isolation & purification , Animals , Cloning, Molecular , Codon , Electrophoresis, Polyacrylamide Gel , Endodeoxyribonucleases/chemistry , Endodeoxyribonucleases/genetics , Enzyme Stability , Escherichia coli/genetics , Escherichia coli/metabolism , Histidine/chemistry , Histidine/genetics , Histidine/metabolism , Humans , Methionine/metabolism , Mice , N-Glycosyl Hydrolases/chemistry , N-Glycosyl Hydrolases/genetics , Oligopeptides/chemistry , Oligopeptides/genetics , Oligopeptides/metabolism , Protein Structure, Tertiary , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/geneticsABSTRACT
Most of the targeting moieties, such as antibody fragments or growth factor domains, used to construct targeted toxins for anticancer therapy derive from secretory proteins. These normally fold in the oxidative environment of the endoplasmic reticulum, and hence their folding in bacterial cells can be quite inefficient. For instance, only low amounts of properly folded antimetastatic chimera constituted by the amino-terminal fragment of human urokinase (ATF) fused to the plant ribosome-inactivating protein saporin could be recovered. ATF-saporin was instead secreted efficiently when expressed in eukaryotic cells protected from autointoxication with neutralizing anti-saporin antibodies. Pichia pastoris is a microbial eukaryotic host where these domains can fold into a transport-competent conformation and reach the extracellular medium. We show here that despite some host toxicity codon-usage optimization greatly increased the expression levels of active saporin but not those of an active-site mutant SAP-KQ in GS115 (his4) strain. The lack of any toxicity associated with expression of the latter confirmed that toxicity is due to saporin catalytic activity. Nevertheless, GS115 (his4) cells in flask culture secreted 3.5 mg/L of a histidine-tagged ATF-saporin chimera showing an IC(50) of 6 x 10(-11) M against U937 cells, thus demonstrating the suitability of this expression platform for secretion of toxic saporin-based chimeras.
Subject(s)
Immunotoxins/genetics , Immunotoxins/metabolism , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , Pichia/genetics , Pichia/metabolism , Plant Proteins/biosynthesis , Plant Proteins/genetics , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/genetics , Ribosome Inactivating Proteins, Type 1/biosynthesis , Ribosome Inactivating Proteins, Type 1/genetics , Urokinase-Type Plasminogen Activator/biosynthesis , Urokinase-Type Plasminogen Activator/genetics , Base Sequence , Binding Sites/genetics , Codon/genetics , DNA Primers/genetics , Gene Expression , Humans , Models, Biological , Mutagenesis, Site-Directed , N-Glycosyl Hydrolases/toxicity , Plant Proteins/toxicity , Protein Processing, Post-Translational , Recombinant Fusion Proteins/toxicity , Ribosome Inactivating Proteins, Type 1/toxicity , Saporins , Transformation, Genetic , U937 Cells , Urokinase-Type Plasminogen Activator/toxicityABSTRACT
The base excision repair (BER) pathway is mainly responsible for the repair of a vast number of non-bulky lesions produced by alkylation, oxidation or deamination of bases. DNA glycosylases are the key enzymes that recognize damaged bases and initiate BER by catalyzing the cleavage of the N-glycosylic bond between the base and the sugar. Many of the mammalian DNA glycosylases have been identified by a combination of biochemical and bioinformatics analysis. Thus, a mammalian family of three proteins (NEIL1, NEIL2 and NEIL3) that showed homology to the Escherichia coli Fpg/Nei DNA glycosylases was identified. Two of the proteins, NEIL1 and NEIL2 have been thoroughly characterized and shown to initiate BER of a diverse number of oxidized lesions. However, much less is known about NEIL3. The biochemical properties of NEIL3 have not been elucidated. This is mainly due to the difficulty in the expression and purification of NEIL3. Here, we describe the expression and partial purification of full-length human NEIL3 and the expression, purification and characterization of a truncated human core-NEIL3 (amino acids 1-301) that contains the complete E. coli Fpg/Nei-like domain but lacks the C-terminal region.
Subject(s)
N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/isolation & purification , Alkylation , Amino Acid Sequence , Cloning, Molecular , DNA Repair , Humans , Molecular Sequence Data , N-Glycosyl Hydrolases/chemistryABSTRACT
The Cg1547 protein of Corynebacterium glutamicum ATCC 13032 is a member of the LacI/GalR family of DNA-binding transcriptional regulators. A defined deletion in the cg1547 gene, now designated uriR (uridine utilization regulator), resulted in the mutant strain C. glutamicum KB1547. Comparison of gene expression levels in C. glutamicum KB1547 and the wild-type strain revealed enhanced expression of the uriR operon genes cg1546 (ribokinase), cg1545 (uridine transporter) and cg1543 (uridine-preferring nucleoside hydrolase). Gene expression of the uriR operon was stimulated by the presence of either uridine or ribose. Growth assays with C. glutamicum mutants showed that functional Cg1543 and Cg1545 proteins are essential for the utilization of uridine as the sole carbon source. Transcriptional regulation of the uriR operon is mediated by a 29 bp palindromic sequence composed of two catabolite-responsive element (cre)-like sequences and located in between the mapped -10 promoter region and the start codon of uriR. A similar cre sequence was detected in the upstream region of rbsK2 (cg2554), coding for a second ribokinase in C. glutamicum ATCC 13032. DNA band-shift assays with a streptavidin-tagged UriR protein and labelled oligonucleotides including the cre-like sequences of uriR and rbsK2 demonstrated the specific binding of the purified regulator in vitro. Whole-genome DNA microarray hybridizations comparing the gene expression in C. glutamicum KB1547 with that of the wild-type strain revealed that UriR is a pathway-specific repressor of genes involved in uridine utilization in C. glutamicum.
Subject(s)
Bacterial Proteins/metabolism , Corynebacterium glutamicum/metabolism , Repressor Proteins/metabolism , Uridine/metabolism , Bacterial Proteins/genetics , Base Sequence , Binding Sites , Corynebacterium glutamicum/genetics , Corynebacterium glutamicum/growth & development , DNA, Bacterial/metabolism , Electrophoretic Mobility Shift Assay , Gene Expression Profiling , Gene Order , Membrane Transport Proteins/biosynthesis , Molecular Sequence Data , N-Glycosyl Hydrolases/biosynthesis , Operon , Phosphotransferases (Alcohol Group Acceptor)/biosynthesis , Promoter Regions, Genetic , Protein Binding , Repressor Proteins/geneticsABSTRACT
Type 2 ribosome inactivating proteins (RIPs) include some potent plant toxins, among which ricin from Ricinus communis and abrin from Abrus precatorius seeds, have been known for more than a century. Two other type 2 RIPs belong to this class of proteins, both isolated from plants of the same family (Passifloraceae), modeccin and volkensin, from Adenia digitata and Adenia volkensii roots, respectively. Volkensin is probably the most potent plant toxin known, with an LD50 for rats of 50-60 ng/kg. Here we report the cloning, expression and renaturation of recombinant volkensin B chain. Furthermore, starting from separately expressed A and B chains, a co-association procedure was set-up, leading to in vitro heterodimeric volkensin reconstitution. The recombinant heterodimer was characterized by N-terminal sequence analysis and its hemagglutinating activity assessed. In parallel, we have explored the carbohydrate-binding properties of native volkensin with the aim to correlate toxin-specific properties (i.e., axonal transport along neurons) to lectin's sugar-binding preferences.
Subject(s)
N-Glycosyl Hydrolases/biosynthesis , Plant Lectins/biosynthesis , Chromatography, Affinity , Cloning, Molecular , Dimerization , Electrophoresis, Polyacrylamide Gel , Enzyme-Linked Immunosorbent Assay , Glycoproteins/metabolism , Hemagglutination Tests , Humans , N-Glycosyl Hydrolases/chemistry , Plant Lectins/chemistry , Protein Folding , Recombinant Proteins/biosynthesis , Recombinant Proteins/chemistry , Ribosome Inactivating Proteins, Type 2ABSTRACT
Pfs expression is required for several metabolic pathways and limits the production of autoinducer-2, a molecule proposed to play a central role in interspecies quorum sensing. The present study reveals physiological conditions and promoter DNA elements that regulate Escherichia coli pfs transcription. Pfs transcription is shown to rely on both sigma 70 and sigma 38 (rpoS), and the latter is subject to induction that increases pfs expression. Transcription is maximal as the cells approach stationary phase, and this level can be increased by salt stress through induction of sigma 38-dependent expression. The pfs promoter is shown to contain both positive and negative elements, which can be used by both forms of RNA polymerase. The negative element is contained within the overlapping dgt promoter, which is involved in purine metabolism. Consideration of the physiological roles of sigma 38 and dgt leads to a model for how autoinducer production is controlled under changing physiological conditions.
Subject(s)
Escherichia coli/physiology , Gene Expression Regulation, Bacterial , Homoserine/analogs & derivatives , N-Glycosyl Hydrolases/genetics , N-Glycosyl Hydrolases/physiology , Purines/metabolism , Transcription, Genetic , Bacterial Proteins/metabolism , DNA-Directed RNA Polymerases/metabolism , Escherichia coli/genetics , Homoserine/biosynthesis , Lactones , N-Glycosyl Hydrolases/biosynthesis , Promoter Regions, Genetic , RNA, Bacterial/analysis , RNA, Bacterial/genetics , RNA, Messenger/analysis , RNA, Messenger/genetics , Sigma Factor/metabolismABSTRACT
BACKGROUND: Glycosyl hydrolase family 1 (GH1) beta-glucosidases have been implicated in physiologically important processes in plants, such as response to biotic and abiotic stresses, defense against herbivores, activation of phytohormones, lignification, and cell wall remodeling. Plant GH1 beta-glucosidases are encoded by a multigene family, so we predicted the structures of the genes and the properties of their protein products, and characterized their phylogenetic relationship to other plant GH1 members, their expression and the activity of one of them, to begin to decipher their roles in rice. RESULTS: Forty GH1 genes could be identified in rice databases, including 2 possible endophyte genes, 2 likely pseudogenes, 2 gene fragments, and 34 apparently competent rice glycosidase genes. Phylogenetic analysis revealed that GH1 members with closely related sequences have similar gene structures and are often clustered together on the same chromosome. Most of the genes appear to have been derived from duplications that occurred after the divergence of rice and Arabidopsis thaliana lineages from their common ancestor, and the two plants share only 8 common gene lineages. At least 31 GH1 genes are expressed in a range of organs and stages of rice, based on the cDNA and EST sequences in public databases. The cDNA of the Os4bglu12 gene, which encodes a protein identical at 40 of 44 amino acid residues with the N-terminal sequence of a cell wall-bound enzyme previously purified from germinating rice, was isolated by RT-PCR from rice seedlings. A thioredoxin-Os4bglu12 fusion protein expressed in Escherichia coli efficiently hydrolyzed beta-(1,4)-linked oligosaccharides of 3-6 glucose residues and laminaribiose. CONCLUSION: Careful analysis of the database sequences produced more reliable rice GH1 gene structure and protein product predictions. Since most of these genes diverged after the divergence of the ancestors of rice and Arabidopsis thaliana, only a few of their functions could be implied from those of GH1 enzymes from Arabidopsis and other dicots. This implies that analysis of GH1 enzymes in monocots is necessary to understand their function in the major grain crops. To begin this analysis, Os4bglu12 beta-glucosidase was characterized and found to have high exoglucanase activity, consistent with a role in cell wall metabolism.
Subject(s)
Gene Expression Regulation, Plant , Genes, Plant , N-Glycosyl Hydrolases/genetics , Oryza/genetics , beta-Glucosidase/genetics , Evolution, Molecular , Expressed Sequence Tags , N-Glycosyl Hydrolases/biosynthesis , Oryza/enzymology , Phylogeny , Pseudogenes , beta-Glucosidase/biosynthesisABSTRACT
rihC is one of a group of three ribonucleoside hydrolases found in Escherichia coli (E. coli). The enzyme catalyzes the hydrolysis of selected nucleosides to ribose and the corresponding base. A family of Vmax/Km kinetic isotope effects using uridine labeled with stable isotopes, such as 2H, 13C, and 15N, were determined by liquid chromatography/mass spectrometry (LC/MS). The kinetic isotope effects were 1.012+/-0.006, 1.027+/-0.005, 1.134+/-0.007, 1.122+/-0.008, and 1.002+/-0.004 for [1'-13C], [1-15N], [1'-2H], [2'-2H], and [5'-2H2] uridine, respectively. A transition state based upon a bond-energy bond-order vibrational analysis (BEBOVIB) of the observed kinetic isotope effects is proposed. The main features of this transition state are activation of the heterocyclic base by protonation of/or hydrogen bonding to O2, an extensively broken C-N glycosidic bond, formation of an oxocarbenium ion in the ribose ring, C3'-exo ribose ring conformation, and almost no bond formation to the attacking nucleophile. The proposed transition state for the prokaryotic E. coli nucleoside hydrolase is compared to that of a similar enzyme isolated from Crithidia fasciculata (C. fasciculata).
Subject(s)
Escherichia coli Proteins/chemistry , Escherichia coli/enzymology , N-Glycosyl Hydrolases/chemistry , Amino Acid Sequence , Animals , Carbon Isotopes/chemistry , Chromatography, High Pressure Liquid , Crithidia fasciculata/enzymology , Crithidia fasciculata/genetics , Deuterium/chemistry , Escherichia coli/genetics , Escherichia coli Proteins/biosynthesis , Escherichia coli Proteins/genetics , Isotopes/chemistry , Kinetics , Mass Spectrometry , Models, Molecular , Molecular Conformation , Molecular Sequence Data , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , Nitrogen Isotopes/chemistry , Recombinant Proteins/chemistry , Recombinant Proteins/isolation & purification , Sequence Homology, Amino Acid , Uridine/chemistryABSTRACT
PttCel9A is a membrane-bound, family 9 glycosyl hydrolase from Populus tremula x tremuloides that is upregulated during secondary cell wall synthesis. The catalytic domain of PttCel9A, Delta(1-105)PttCel9A, was purified, and its activity was compared to TfCel9A and TfCel9B from Thermobifida fusca. Since aromatic amino acids involved in substrate binding at subsites -4, -3, and -2 are missing in PttCel9A, the activity of TfCel9A mutant enzymes W256S, W209A, and W313G was also investigated. Delta(1-105)PttCel9A hydrolyzed a comparatively narrow range of polymeric substrates, and the preferred substrate was (carboxymethyl)cellulose 4M. Moreover, Delta(1-105)PttCel9A did not hydrolyze oligosaccharides shorter than cellopentaose, whereas TfCel9A and TfCel9B hydrolyzed cellotetraose and cellotriose, respectively. These data suggest that the preferred substrates of PttCel9A are long, low-substituted, soluble cellulosic polymers. At 30 degrees C and pH 6.0, the kcat for cellohexaose of Delta(1-105)PttCel9A, TfCel9A, and TfCel9B were 0.023 +/- 0.001, 16.9 +/- 2.0, and 1.3 +/- 0.2, respectively. The catalytic efficiency (kcat/Km) of TfCel9B was 39% of that of TfCel9A, whereas the catalytic efficiency of Delta(1-105)PttCel9A was 0.04% of that of TfCel9A. Removing tryptophan residues at subsites -4, -3, and -2 decreased the efficiency of cellohexaose hydrolysis by TfCel9A. Mutation of W313 to G had the most drastic effect, producing a mutant enzyme with 1% of the catalytic efficiency of TfCel9A. The apparent narrow substrate range and catalytic efficiency of PttCel9A are correlated with a lack of aromatic amino acids in the substrate binding cleft and may be necessary to prevent excessive hydrolysis of cell wall polysaccharides during cell wall formation.
Subject(s)
Cellulose/analogs & derivatives , N-Glycosyl Hydrolases/genetics , N-Glycosyl Hydrolases/metabolism , Plant Proteins/genetics , Plant Proteins/metabolism , Populus/enzymology , Populus/genetics , Recombinant Proteins/chemistry , Sequence Homology, Amino Acid , Amino Acid Sequence , Arabidopsis/enzymology , Calcium Chloride/metabolism , Cations, Divalent/metabolism , Cellulose/metabolism , Chlorides/metabolism , Hydrogen-Ion Concentration , Hydrolysis , Kinetics , Molecular Sequence Data , Mutagenesis, Site-Directed , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/isolation & purification , Oligosaccharides/metabolism , Pichia/enzymology , Pichia/genetics , Plant Proteins/biosynthesis , Plant Proteins/isolation & purification , Polymers/metabolism , Recombinant Proteins/biosynthesis , Substrate Specificity , Tetroses/metabolism , Zinc Compounds/metabolismABSTRACT
Musarmins are type 1 ribosome-inactivating proteins with N-glycosidase activity on the 28 S rRNA that are present in bulbs of Muscari armeniacum L. and Miller at rather low concentrations. In the present work, a cDNA fragment coding for musarmin 1 was sub-cloned and expressed in Escherichia coli. The recombinant protein (rMU1) was synthesised as a polypeptide of 295 amino acids that was delivered to the periplasm and processed. Recombinant musarmin 1 present in the periplam has two forms: insoluble with a molecular mass of 29,423 and soluble with a molecular mass of 29,117 because of a small proteolytic shortening with respect to the insoluble one, presumably in the C-terminal. The yield of protein homogeneous by polyacrylamide gel electrophoresis was 23mgl-1 of bacterial culture. The recombinant musarmin 1 forms isolated from both the soluble and the insoluble (upon refolding) fractions retained full translational inhibitory and 28 S rRNA N-glycosidase activities as compared with the native protein. The recombinant protein displayed great stability towards trypsin, collagenase, rat plasma and rat liver protein extract, but was sensitive to the action of papain and proteinase K. The easy availability and full activity of the recombinant musarmin 1 makes it a good candidate for the preparation of immunotoxins for targeted therapy and for the construction of transgenic plants expressing it as antipathogenic agent.
Subject(s)
Escherichia coli/genetics , Escherichia coli/metabolism , Liliaceae/genetics , Liliaceae/metabolism , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , Plant Roots/genetics , Plant Roots/metabolism , Protein Engineering/methods , Liliaceae/classification , Plant Roots/classification , Recombinant Proteins/biosynthesis , Species SpecificityABSTRACT
Although the type-2 ribosome-inactivating proteins (SNA-I, SNA-V, SNLRP) from elderberry (Sambucus nigra L.) are all devoid of rRNA N-glycosylase activity towards plant ribosomes, some of them clearly show polynucleotide-adenosine glycosylase activity towards tobacco mosaic virus RNA. This particular substrate specificity was exploited to further unravel the mechanism underlying the in planta antiviral activity of ribosome-inactivating proteins. Transgenic tobacco (Nicotiana tabacum L. cv Samsun NN) plants expressing the elderberry ribosome-inactivating proteins were generated and challenged with tobacco mosaic virus in order to analyze their antiviral properties. Although some transgenic plants clearly showed antiviral activity, no clear correlation was observed between in planta antiviral activity of transgenic tobacco lines expressing the different ribosome-inactivating proteins and the in vitro polynucleotide-adenosine glycosylase activity of the respective proteins towards tobacco mosaic virus genomic RNA. However, our results suggest that the in planta antiviral activity of some ribosome-inactivating proteins may rely on a direct mechanism on the virus. In addition, it is evident that the working mechanism proposed for pokeweed antiviral protein cannot be extrapolated to elderberry ribosome-inactivating proteins because the expression of SNA-V is not accompanied by induction of pathogenesis-related proteins.
Subject(s)
Antiviral Agents/pharmacology , N-Glycosyl Hydrolases/physiology , Nicotiana/genetics , Nicotiana/virology , Plant Proteins/physiology , Sambucus nigra/metabolism , Tobacco Mosaic Virus/physiology , Adenine/metabolism , Animals , Antiviral Agents/metabolism , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , N-Glycosyl Hydrolases/metabolism , N-Glycosyl Hydrolases/pharmacology , Plant Diseases/virology , Plant Proteins/biosynthesis , Plant Proteins/genetics , Plant Proteins/pharmacology , Plants, Genetically Modified/genetics , Plants, Genetically Modified/metabolism , Plants, Genetically Modified/virology , RNA, Ribosomal/metabolism , RNA, Viral/metabolism , Rabbits , Recombinant Proteins/genetics , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Recombinant Proteins/pharmacology , Reticulocytes/metabolism , Ribosome Inactivating Proteins, Type 2 , Ribosomes/metabolism , Sambucus nigra/genetics , Nicotiana/metabolism , Tobacco Mosaic Virus/geneticsABSTRACT
A novel ribosome-inactivating protein designated Moschatin from the mature seeds of pumpkin (Cucurbita moschata) has been successively purified to homogeneity, using ammonium sulfate precipitation, CM-cellulose 52 column chromatography, Blue Sepharose CL-6B Affinity column chromatography and FPLC size-exclusion column chromatography. Moschatin is a type 1 RIP with a pI of 9.4 and molecular weight of approximately 29 kD. It is a rRNA N-glycosidase and potently blocked the protein synthesis in the rabbit reticulocyte lysate with a IC50 of 0.26 nM. Using the anti-human melanoma McAb Ng76, a novel immunotoxin Moschatin-Ng76 was prepared successfully and it efficiently inhibited the growth of targeted melanoma cells M21 with a IC50 of 0.04 nM, 1500 times lower than that of free Moschatin. The results implied that Moschatin could be used as a new potential anticancer agent.
Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Cucurbita/chemistry , Immunotoxins/chemistry , Immunotoxins/pharmacology , Melanoma/drug therapy , N-Glycosyl Hydrolases/pharmacology , Seeds/chemistry , Animals , Antineoplastic Agents, Phytogenic/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Cell Line, Tumor , Drug Screening Assays, Antitumor , Humans , Immunoglobulin G/immunology , Immunotoxins/immunology , Immunotoxins/isolation & purification , Molecular Weight , N-Glycosyl Hydrolases/antagonists & inhibitors , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/chemistry , Rabbits , Ribosome Inactivating Proteins , Ribosomes/drug effectsABSTRACT
Activation-induced cytidine deaminase (AID) is a 'master molecule' in immunoglobulin (Ig) class-switch recombination (CSR) and somatic hypermutation (SHM) generation, AID deficiencies are associated with hyper-IgM phenotypes in humans and mice. We show here that recessive mutations of the gene encoding uracil-DNA glycosylase (UNG) are associated with profound impairment in CSR at a DNA precleavage step and with a partial disturbance of the SHM pattern in three patients with hyper-IgM syndrome. Together with the finding that nuclear UNG expression was induced in activated B cells, these data support a model of CSR and SHM in which AID deaminates cytosine into uracil in targeted DNA (immunoglobulin switch or variable regions), followed by uracil removal by UNG.
Subject(s)
Cytidine Deaminase/immunology , DNA Glycosylases , Immune Complex Diseases/genetics , Immunoglobulin Class Switching/genetics , N-Glycosyl Hydrolases/deficiency , Somatic Hypermutation, Immunoglobulin/genetics , Adult , Amino Acid Sequence , Animals , Base Sequence , Child , Cytidine Deaminase/genetics , Gene Expression Regulation/genetics , Gene Expression Regulation/immunology , Humans , Immune Complex Diseases/immunology , Immunoglobulin Class Switching/immunology , Immunoglobulin M/genetics , Immunoglobulin M/immunology , Mice , Mice, Inbred BALB C , Molecular Sequence Data , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/genetics , N-Glycosyl Hydrolases/immunology , Point Mutation , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , Sequence Analysis, DNA , Somatic Hypermutation, Immunoglobulin/immunology , Uracil-DNA GlycosidaseABSTRACT
A novel human cytosolic flavin reductase, Nr1, was recently described that contains FMN, FAD, and NADPH cofactors. Though the targets of the related NADPH-dependent flavoprotein reductases, cytochrome P450 reductase, methionine synthase reductase, and nitric oxide synthase, are known, the cellular function of Nr1 is not clear. To explore expression and regulation of Nr1, we cloned fre-1, the Caenorhabditis elegans ortholog of Nr1, and discovered that it is transcribed as a bicistronic pre-mRNA together with dcs-1, the ortholog of the recently described scavenger mRNA decapping enzyme. We used the novel substrate, 7meGpppBODIPY, to demonstrate that DCS-1 has low micromolar specificity for guanine ribonucleotides with the 7me modification, whereas trimethylated G substrates are poor competitors. Contrary to earlier classification, DCS-1 is not a pyrophosphatase but a distant member of the Hint branch of the histidine triad superfamily of nucleotide hydrolases and transferases. These observations are consistent with the hypothesis that DCS-1 homologs may function in the metabolism of capped oligonucleotides generated following exosome-dependent degradation of short-lived mRNA transcripts. We find that fre-1 and dcs-1 are coordinately expressed through worm development, are induced by heat shock, and have a nearly identical expression profile in human tissues. Furthermore, immunocytochemical analysis of the endogenous proteins in COS cells indicates that both are present in the nucleus and concentrated in a distinct perinuclear structure. Though no connection between these enzymes had been anticipated, our data and data from global expression and protein association studies suggest that the two enzymes jointly participate in responses to DNA damage, heat shock, and other stresses.
Subject(s)
Caenorhabditis elegans/chemistry , FMN Reductase/chemistry , FMN Reductase/genetics , Hydrolases/genetics , N-Glycosyl Hydrolases/biosynthesis , N-Glycosyl Hydrolases/chemistry , Saccharomyces cerevisiae Proteins/biosynthesis , Saccharomyces cerevisiae Proteins/chemistry , Amino Acid Sequence , Animals , Animals, Genetically Modified , Boron Compounds/pharmacology , COS Cells , Caenorhabditis elegans Proteins , Cell Nucleus/metabolism , Cloning, Molecular , DNA Damage , Histidine/chemistry , Hot Temperature , Humans , Hydrolases/chemistry , Immunohistochemistry , Kinetics , Molecular Sequence Data , NADP/metabolism , Operon , Pyrophosphatases/metabolism , RNA, Messenger/metabolism , Recombinant Proteins/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Amino Acid , Time Factors , Tissue DistributionABSTRACT
The nucleoside hydrolases (NHs) are a family of nucleoside-modifying enzymes. They play an important role in the purine-salvage pathway of many pathogenic organisms which are unable to synthesize purines de novo. Although well characterized in protozoan parasites, their precise function and mechanism remain unclear in other species. For the first time, NHs from Caenorhabditis elegans and Campylobacter jejuni, which are representatives of mesozoa and bacteria, respectively, have been cloned and purified. Steady-state kinetics indicate a different substrate-specificity profile to previously described hydrolases. Native diffraction data sets were collected from crystals of NH from each organism. The hexagonal crystals (space group P6(2)22 or P6(4)22) of NH from C. elegans diffracted to a resolution of 2.8 A, while the data set from the orthorhombic crystals (space group I222 or I2(1)2(1)2(1)) of NH from C. jejuni could be processed to 1.7 A resolution. The unit-cell parameters were a = b = 102.23, c = 117.27 A in the former case and a = 101.13, b = 100.13, c = 81.37 A in the latter.