ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: The flowers of Nyctanthes arbor-tristis (L.) heals mouth ulcers. Its tinctures promote gastric secretions, and improve lung expectoration when taken orally. It has traditionally been used to treats scabies and other skin problems. The leaves of NAT(L.) plant are used in Ayurvedic medicine to treat sciatica, chronic fever, rheumatism, internal worm infections, and as a laxative, diaphoretic, and diuretic. The bark used in treatment of snakebite and bronchitis. In addition to traditional uses, pharmacologically this plant has potent antimalarial, antiarthritic, anticancer and antidiabetic activity. However, the mechanistic antiproliferative potentials of NAT(L.) flower as anticancer therapeutics has not yet been explored. AIM OF THE STUDY: The current study is based on a broad range of scientific literature that highlights the nutritional and therapeutic benefits of NAT (L.). Present investigation was carried out to determine the therapeutic efficacy of NAT (L.) against breast adenocarcinoma cells and T-cell lymphoma. MATERIALS AND METHODS: The ethyl-acetate extract of NAT(L.) was tested against breast cancer cells to assess the anticancer potential. To evaluate apoptosis, intracellular ROS levels and mitochondrial dynamics, fluorescence microscopy and flow cytometry were employed. Additionally, cell cycle analysis and western blotting were also performed. Furthermore, in vivo antitumor efficacy of flower extracts was investigated in T-cell lymphoma-bearing BALB/c mice model. RESULTS: Our present study revealed that NAT (L.) exert anticancer activity against breast cancer cells effectively at IC50 320 µg/ml while having less impact on normal cells with IC50 more than 480 µg/ml. Fluorescence imaging showed that NAT (L.) treatment elicits a concentration-dependent rise in the occurrence of apoptotic cell deaths with altered mitochondrial dynamics and was subsequently confirmed by flow cytometry. Further, flow cytometric analysis delineates ethyl acetate flower extract exposure promotes arrest of cells in S phase of the cell cycle. The differential expression of apoptotic proteins such as Bax, Bcl-2, cleaved PARP-1, cleaved caspase 3, Cytochrome-c, p53 and VEGF A were influenced by NAT (L.) treatment. The in vivo antitumor activity study delineates that NAT(L.) therapy significantly increased the life span of T-cell lymphoma bearing mice while reducing tumor load and belly size growth pattern without causing significant other distinct side effects as evident by histopathological studies. CONCLUSION: Our current findings unveil that NAT(L.) ethyl acetate flower extract potentially induces mitochondrial pathway of apoptosis, promote cell cycle arrest, reduces tumor load of mice, enhances survivability and could be a promising agent against the triple negative breast cancer and lymphoma.
Subject(s)
Adenocarcinoma , Antineoplastic Agents, Phytogenic , Apoptosis , Breast Neoplasms , Flowers , Lymphoma, T-Cell , Mitochondria , Plant Extracts , Animals , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Flowers/chemistry , Mitochondria/drug effects , Female , Lymphoma, T-Cell/drug therapy , Lymphoma, T-Cell/pathology , Breast Neoplasms/drug therapy , Breast Neoplasms/pathology , Humans , Apoptosis/drug effects , Antineoplastic Agents, Phytogenic/pharmacology , Antineoplastic Agents, Phytogenic/therapeutic use , Mice , Adenocarcinoma/drug therapy , Adenocarcinoma/pathology , Mice, Inbred BALB C , Cell Line, Tumor , Oleaceae/chemistry , Reactive Oxygen Species/metabolism , Membrane Potential, Mitochondrial/drug effects , Cell Proliferation/drug effects , Xenograft Model Antitumor AssaysABSTRACT
The importance of medicinal plants for the treatment of different diseases is high from the aspects of the pharmaceutical industry and traditional healers. The present study involves nine different medicinal plants, namely, Neolamarckia cadamba, Nyctanthes arbor-tristis, Pogostemon benghalensis, Equisetum debile, Litsea monopetala, Spilanthes uliginosa, Desmostachya bipinnata, Mallotus philippensis, and Phoenix humilis, collected from Chitwan district of Nepal for biochemical analysis followed by the isolation of active plant fractions from the bioactive plant extract. The methanolic extracts of roots, barks, seeds, seed cover, and the other aerial parts of plants were used for the phytochemical analysis and biological activities. The DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay was adopted to evaluate the antioxidant activity. Antibacterial activity was evaluated using the agar well diffusion method. The antidiabetic activity was studied by the α-amylase enzyme inhibition assay. The highest antioxidant activity was observed in extracts of Nyctanthes arbor-tristis followed by Mallotus philippensis (seed cover), Pogostemon benghalensis, Litsea monopetala, Phoenix humilis, and Neolamarckia cadamba with IC50 values of 27.38 ± 1.35, 32.08 ± 2.81, 32.75 ± 2.13, 33.82 ± 1.07, 40.14 ± 0.93, and 50.44 ± 3.75 µg/mL, respectively. The highest antidiabetic activity was observed in extracts of Phoenix humilis followed by Desmostachya bipinnata and Pogostemon benghalensis with IC50 values of 95.69 ± 6.97, 99.24 ± 12.6, and 106.3 ± 12.89 µg/mL, respectively. The mild α-amylase enzyme inhibition was found in extracts of Nyctanthes arbor-tristis, Spilanthes uliginosa Swartz, Litsea monopetala, and Equisetum debile showing IC50 values of 110.4 ± 7.78, 115.98 ± 10.24, 149.83 ± 8.3, and 196.45 ± 6.04 µg/mL, whereas Mallotus Philippensis (seed cover), Mallotus philippensis (seed), and Desmostachya bipinnata showed weak α-amylase inhibition with IC50 values of 208.87 ± 1.76, 215.41 ± 2.09, and 238.89 ± 9.27 µg/mL, respectively. The extract of Nyctanthes arbor-tristis showed high zones of inhibition against S. aureus (ATCC 25923) and E. coli (ATCC 25922) of ZOI 26 and 22 mm, respectively. The chemical constituents isolated from the active plant Nyctanthes arbor-tristis were subjected to GCMS analysis where the major chemical compounds were 11,14,17-eicosatrienoic acid and methyl ester. These results support the partial scientific validation for the traditional uses of these medicinal plants in the treatment of diabetes and infectious diseases by the people living in different communities of Chitwan, Nepal.
Subject(s)
Oleaceae , Plants, Medicinal , Humans , Nepal , Antioxidants/pharmacology , Antioxidants/chemistry , Escherichia coli , Staphylococcus aureus , Plant Extracts/chemistry , Oleaceae/chemistry , Hypoglycemic Agents , alpha-AmylasesABSTRACT
Nyctanthes arbor-tristis, alias "Vishnu Parijat," is a medicinal plant used to treat various inflammation-associated ailments and to combat innumerable infections in the traditional system of medicine. In the present study, we collected the samples of N. arbor-tristis from the lower Himalayan region of Uttarakhand, India, and carried out their molecular identification through DNA barcoding. To examine the antioxidant and antibacterial activities, we prepared the ethanolic and aqueous extracts (from flowers and leaves) and performed their phytochemical analysis by using different qualitative and quantitative approaches. The phytoextracts showed marked antioxidant potential, as revealed by a comprehensive set of assays. The ethanolic leaf extract showed marked antioxidant potential towards DPPH, ABTS, and NO scavenging (IC50 = 30.75 ± 0.006, 30.83 ± 0.002, and 51.23 ± 0.009 µg/mL, respectively). We used TLC-bioautography assay to characterize different antioxidant constituents (based on their Rf values) in the chromatograms ran under different mobile phases. For one of the prominent antioxidant spots in TLC bioautography, GC-MS analysis identified cis-9-hexadecenal and n-hexadecanoic acid as the major constituents. Furthermore, in antibacterial study, the ethanolic leaf extract showed marked activity against Aeromonas salmonicida (113.40 mg/mL of extract was equivalent to 100 µg/mL of kanamycin). In contrast, the ethanolic flower extract showed considerable antibacterial activity against Pseudomonas aeruginosa (125.85 mg/mL of extract ≡100 µg/mL of kanamycin). This study presents the phylogenetic account and unravels the antioxidant-related properties and antibacterial potential of N. arbor-tristis.
Subject(s)
Oleaceae , Plant Extracts , Plant Extracts/pharmacology , Plant Extracts/chemistry , Antioxidants/pharmacology , Antioxidants/chemistry , Phylogeny , Anti-Bacterial Agents/pharmacology , Kanamycin , Oleaceae/chemistry , Phytochemicals/pharmacology , Plant LeavesABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Nyctanthes arbor-tristis Linn. is native to Indo-Pak sub-continent and has high medicinal values in Ayureda. This plant has been used traditionally for the treatment of sciatica, rheumatism, chronic fever, diabetes, snakebite, dysentery, cachexia and cancer. Studies have shown many pharmacological properties such as anti-cancer efficacy against Dalton's ascetic lymphoma, cytotoxicity against T-cell leukemia, anti-inflammatory, anti-diabetic and anti-oxidant effects. AIM OF THE STUDY: Aim of the study was to explore the anti-inflammatory and anti-proliferative potential of N. arbor-tristis. MATERIAL AND METHODS: Ethanol extract of fresh and uncrushed aerial parts of N. arbor-tristis was used in the present study. A new compound nyctanthesin A was isolated following a bioactivity-guided fractionation and chromatographic separations. Its chemical structure was elucidated through spectral studies including 1D, 2D-NMR experiments and HREIMS. The intracellular reactive oxygen species (ROS) and nitric oxide (NO) generation from phagocytes were detected by chemiluminescence technique and Griess method, respectively. TNF-α and TGF-ß production was quantified by ELISA. Anti-lymphoma and cytotoxic activities were assessed by alamar blue and MTT assays, respectively. The transcription and protein expression level of Bcl-2, COX-2, p38 MAPK, PDL-1, NF-κB, c-Myc and PNF-κB was performed by qRT-PCR and protein blot assays, respectively. RESULTS: Petroleum ether insoluble fraction of the ethanol extract of fresh and uncrushed aerial parts of N. arbor-tristis revealed anti-inflammatory potential by inhibiting ROS. A previously undescribed compound nyctanthesin A was isolated from this fraction and characterized by UV, IR, NMR and HREIMS. It showed significant anti-inflammatory property by inhibiting ROS, NO and TNF-α production. The strong anti-proliferative effects on B- cell lymphoma cells, DOHH2 and Raji, revealed its anti-lymphoma potential along with non-toxic profile against BJ and NIH-3T3 fibroblast cells of normal origin. The qRT-PCR results showed marked inhibition of Bcl-2, COX-2, p38 MAPK, PDL-1, c-Myc, NF-κB, and PNF-κB at transcription level in DOHH2 cells with comparatively lesser but significant effects in Raji cells, where the expression of Bcl-2 gene was not affected. The protein expression of PNF-κB in DOHH2 cells was inhibited by 66% (P < 0.05) and COX-2 in both cell lines was inhibited by 50% (P < 0.05) at 60 µg/mL. A moderate non-significant inhibition of TGF-ß (â¼20%) was observed in both cell lines at 100 µg/mL CONCLUSIONS: Scientific evidences reported here validate the anti-inflammatory and anti-cancer potential of the plant.
Subject(s)
Lymphoma, Non-Hodgkin , Oleaceae , Anti-Inflammatory Agents/pharmacology , Cyclooxygenase 2/genetics , Ethanol , Humans , Lipopolysaccharides , Lymphoma, Non-Hodgkin/drug therapy , NF-kappa B/metabolism , Nitric Oxide/metabolism , Oleaceae/chemistry , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Proto-Oncogene Proteins c-bcl-2 , Reactive Oxygen Species , Transforming Growth Factor beta , Tumor Necrosis Factor-alpha , p38 Mitogen-Activated Protein KinasesABSTRACT
Oleuropein (OLE) is the main bioactive ingredient in the leaves of the olive plant Olea europaea L. (Oleaceae), which has proven beneficial due to the antiinflammatory, antiatherogenic, anticancer, antimicrobial, and antiviral effects. This study aimed to investigate the antihypertensive and vasodilator potential of OLE by analyzing its acute effects on spontaneous atrial contractions and vasomotor responses of the isolated thoracic aorta in rats. We showed that the application of OLE induces negative chronotropic and inotropic effects on the heart. OLE also causes mild aortic vasodilation given that the maximal reduction in tension of intact aortic rings precontracted with phenylephrine was approximately 30%. This vasodilation is likely dependent on the nitric oxide released from the endothelium based on the effect obtained on denuded and phenylephrine precontracted aortic rings and responses reordered following vasoconstriction induced by high concentrations of K+ and heparin. Our findings provide a basis for further testing of OLE cardiovascular effects, which may lead to subsequent clinical research for its application in the treatment of hypertension and heart disease.
Subject(s)
Antihypertensive Agents/pharmacology , Endothelium, Vascular/drug effects , Heart Atria/drug effects , Iridoid Glucosides/pharmacology , Vasodilator Agents/administration & dosage , Animals , Antihypertensive Agents/therapeutic use , Aorta, Thoracic/drug effects , Drug Evaluation, Preclinical , Endothelium, Vascular/metabolism , Heart Atria/metabolism , Humans , Hypertension/drug therapy , Iridoid Glucosides/therapeutic use , Male , Models, Animal , Nitric Oxide/metabolism , Oleaceae/chemistry , Plant Leaves/chemistry , Rats , Vasoconstriction/drug effects , Vasodilation/drug effectsABSTRACT
Sweet osmanthus (Osmanthus fragrans Lour.) (OF) is one of the ten most famous flowers in China for its unique and delicate fragrance. A combined solid-phase microextraction and solvent-assisted flavor evaporation method was used to accurately capture the overall aromatic profile and characterize the predominant odorants of fresh osmanthus with the help of gas chromatography (GC)-olfactometry and comprehensive two-dimensional GC-quadrupole time-of-flight mass spectrometry (GC × GC-QTOF-MS). Twenty-six volatiles were identified for the first time in OF. A total of 23 potent odorants, dominated by monoterpene oxides and C6 aliphatic aldehydes, were identified. The efficacy of pectinase, ß-glucosidase, and their combination on the aroma enhancement of OF was evaluated by quantitation of the target aroma components using GC-triple quadrupole-MS. The total concentration of key aroma components increased in all three enzyme treatment groups, and the increase was more significant in two ß-glucosidase-treated groups. Changes in odor activity values and odor spectrum values of key odorants indicated that the pectinase-treated sample had more prominent floral, green, and potato-like scents. In contrast, the ß-glucosidase-treated sample had more dominant floral, woody, almond-like, and fruity notes but less green odor, which was confirmed by sensory evaluation. ß-Glucosidase and pectinase complement one another very well, and together, promote a remarkable aroma enhancement in OF.
Subject(s)
Flavoring Agents/chemistry , Odorants/analysis , Oleaceae/chemistry , Polygalacturonase/chemistry , beta-Glucosidase/chemistry , Biocatalysis , China , Flavoring Agents/isolation & purification , Flowers/chemistry , Food Handling , Gas Chromatography-Mass Spectrometry , Solid Phase Microextraction , Volatile Organic Compounds/chemistry , Volatile Organic Compounds/isolation & purificationABSTRACT
Background: Preterm birth is a known leading cause of neonatal mortality and morbidity. The underlying causes of pregnancy-associated complications are numerous, but infection and inflammation are the essential high-risk factors. However, there are no safe and effective preventive drugs that can be applied to pregnant women. Objective: The objectives of the study were to investigate a natural product, Abeliophyllum distichum leaf (ADL) extract, to examine the possibility of preventing preterm birth caused by inflammation. Methods: We used a mouse preterm birth model by intraperitoneally injecting lipopolysaccharides (LPS). ELISA, Western blot, real-time PCR and immunofluorescence staining analyses were performed to confirm the anti-inflammatory efficacy and related mechanisms of the ADL extracts. Cytotoxicity and cell death were measured using Cell Counting Kit-8 (CCK-8) analysis and flow cytometer. Results: A daily administration of ADL extract significantly reduced preterm birth, fetal loss, and fetal growth restriction after an intraperitoneal injection of LPS in mice. The ADL extract prevented the LPS-induced expression of TNF-α in maternal serum and amniotic fluid and attenuated the LPS-induced upregulation of placental proinflammatory genes, including IL-1ß, IL-6, IL-12p40, and TNF-α and the chemokine gene CXCL-1, CCL-2, CCL3, and CCL-4. LPS-treated THP-1 cell-conditioned medium accelerated trophoblast cell death, and TNF-α played an essential role in this effect. The ADL extract reduced LPS-treated THP-1 cell-conditioned medium-induced trophoblast cell death by inhibiting MAPKs and the NF-κB pathway in macrophages. ADL extract prevented exogenous TNF-α-induced increased trophoblast cell death and decreased cell viability. Conclusions: We have demonstrated that the inhibition of LPS-induced inflammation by ADL extract can prevent preterm birth, fetal loss, and fetal growth restriction.
Subject(s)
Glucosides/chemistry , Lipopolysaccharides/toxicity , Macrophages/drug effects , Macrophages/metabolism , Oleaceae/chemistry , Phenols/chemistry , Plant Extracts/therapeutic use , Plant Leaves/chemistry , Premature Birth/chemically induced , Premature Birth/prevention & control , Tumor Necrosis Factor-alpha/pharmacology , Animals , Cell Death/drug effects , Chromatography, High Pressure Liquid , Female , Male , Mice , Trophoblasts/cytology , Trophoblasts/metabolismABSTRACT
White fringetree is a host for the invasive emerald ash borer (EAB) but is of lower quality than the related and highly susceptible black ash. Field observations suggest that host trees grown in full sun are more resistant to EAB than those in shade, however the impact of light limitation on chemical defenses has not been assessed. We quantified constitutive and jasmonate-induced phloem defenses and growth patterns of white fringetree and black ash under differential light conditions and related them to EAB larval performance. White fringetree had significantly lower constitutive and induced activities of peroxidase, polyphenol oxidase, ß-glucosidase, chitinase and lignin content, but significantly higher gallic acid equivalent soluble phenolic, soluble sugar, and oleuropein concentrations compared to black ash. Multivariate analyses based on tissue chemical attributes displayed clear separation of species and induced defense responses. Further, EAB performed significantly worse on white fringetree than black ash, consistent with previous studies. Light limitation did not impact measured defenses or EAB larval performance, but it did decrease current year growth and increase photosynthetic efficiency. Overall our results suggest that phenolic profiles, metabolite abundance, and growth traits are important in mediating white fringetree resistance to EAB, and that short-term light limitation does not influence phloem chemistry or larval success.
Subject(s)
Coleoptera/physiology , Cyclopentanes/metabolism , Fraxinus/chemistry , Oleaceae/chemistry , Oxylipins/metabolism , Plant Extracts/chemistry , Animals , Behavior, Animal , Catechol Oxidase/metabolism , Chitinases/metabolism , Fraxinus/metabolism , Gallic Acid/metabolism , Iridoid Glucosides/metabolism , Larva , Light , Lignin/metabolism , Oleaceae/metabolism , Phenols/metabolism , Phloem/metabolism , Photosynthesis , Sugars/metabolism , beta-Glucosidase/metabolismABSTRACT
Abeliophyllum distichum Nakai is a Korean endemic plant of the Oleaceae family that contains acteoside, a glycosylated caffeic acid, with neuroprotective, antiinflammatory and antibacterial properties. Previous studies, involving Accelerated Chromatographic Isolation, a highperformance liquid chromatographyphotodiode array detector and a liquid chromatographmass selective detector, isolated and identified acteoside in A. distichum (AAD) and documented its antioxidant and antiinflammatory activities. The aim of the present study was to determine whether AAD could protect from DNA damage by reducing oxidative stress. AAD treatment protected plasmid DNA against damage to DNA doublestrands induced by reactive oxygen species (ROS) and decreased the levels of phosphorylated p53 and γH2AX in ROStreated NIH 3T3 cells. These findings suggested that AAD could reduce ROSmediated cellular damage and may represent an effective, natural antioxidant with the ability to protect genetic material.
Subject(s)
Antioxidants/pharmacology , DNA Damage/drug effects , Glucosides/pharmacology , Oleaceae/chemistry , Phenols/pharmacology , Animals , Histones/metabolism , Mice , NIH 3T3 Cells , Phosphorylation , Plant Extracts/chemistry , Reactive Oxygen Species/metabolism , Tumor Suppressor Protein p53/metabolismABSTRACT
Osmanthus fragrans var. aurantiacus has been used for the treatment of menopausal pain, foul breath, and intestinal bleeding. Four phenylpropyl triterpenoids, 3-O-trans-p-coumaroyltormentic acid (1), 3ß-trans-p-coumaroyloxy-2α-hydroxyl-urs-12-en-28-oic acid (2), 3ß-cis-p-coumaroyloxy-2α-hydroxyl-urs-12-en-28-oic acid (3), 3-O-cis-coumaroylmaslinic acid (4), were isolated from the leaves of O. fragrans var. aurantiacus and the inhibitory effect on nitric oxide (NO) production in lipopolysaccharide (LPS)-induced macrophages were evaluated. Among them, compounds 2-4 concentration dependently showed NO production inhibitory effects. To determine the signaling factors involved in the inhibition of NO production by compounds 2-4, we assessed anti-inflammatory activity. Western blot analysis revealed compounds 2-4 significantly decreased the expression of LPS-stimulated protein, inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, nuclear factor-kappa B (NF-κB) and phosphorylated extracellular regulated kinase (pERK)1/2. Also, compounds 2-4 downregulated tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, IL-6 and IL-8 levels in LPS-induced macrophages and colonic epithelial cells. This study demonstrated that phenylpropyl triterpenoids 2-4 isolated from O. fragrans var. aurantiacus leaves can be used as potential candidates for the prevention and treatment of inflammatory bowel disease.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Oleaceae/chemistry , Triterpenes/pharmacology , Animals , Anti-Inflammatory Agents/chemistry , Anti-Inflammatory Agents/isolation & purification , Cell Survival/drug effects , Cyclooxygenase 2/drug effects , Cytokines/drug effects , HT29 Cells , Humans , Lipopolysaccharides/toxicity , Mice , Mitogen-Activated Protein Kinase 1/drug effects , Mitogen-Activated Protein Kinase 3/drug effects , NF-kappa B p50 Subunit/drug effects , Nitric Oxide/metabolism , Nitric Oxide Synthase Type II/drug effects , Plant Leaves/chemistry , RAW 264.7 Cells , Triterpenes/chemistry , Triterpenes/isolation & purificationABSTRACT
Triterpenic acids possess rich biological activity. Due to slight differences in structure and polarity, the simultaneous determination of isomeric triterpenic acids is challenging. In the present work, a simple and effective approach to chromatographic separation of such compounds based on conventional C18 stationary phase with gradient elution was developed, which allowed the simultaneous separation of eleven analytes including euscaphic, arjunic, tormentic, arjunolic, asiatic, pomolic, maslinic, corosolic, oleanolic, ursolic and 2-Epi tormentic acid (internal standard). This approach with mass spectrometric detection and ultrasonic extraction was fast, sensitive and accurate for analyzing isomeric triterpenic acids in O. fragrans fruits with a toal duration of the analytical cycle (including pretreatment) within one hour. The LODs lie in ranges of 0.8-12 ng/mL (30 ng/mL for asiatic and corosolic acid). The developed method was validated and successfully applied in ten batches of O. fragrans fruits, which could reflect the detail content difference of triterpenic acid components.
Subject(s)
Chromatography, High Pressure Liquid/methods , Oleaceae/chemistry , Tandem Mass Spectrometry/methods , Triterpenes/analysis , Fruit/chemistry , Isomerism , Limit of Detection , Oleanolic Acid/analysis , Sensitivity and Specificity , Triterpenes/chemistryABSTRACT
This work reports on the preparation of a drying process from the ethanolic extract of Muirapuama and its characterization through green analytical techniques. The spray-drying processes were performed by using ethanolic extract in a ratio of 1:1 extract/excipient and 32 factorial design. The properties of dried powder were investigated in terms of total flavonoid content, moisture content, powder yield, and particle size distribution. An analytical eco-scale was applied to assess the greenness of the developed protocol. Ultra-high performance liquid chromatography (UHPLC)with reduced solvent consumption in the analysis was compared to the conventional HPLC method. A Fourier transform near-infrared (FT-NIR) spectroscopic method was applied based on the principal component scores for the prediction of extract/excipient mixtures and partial least squares regression model for quantitative analysis. NIR spectroscopy is an economic, powerful, and fast methodology for the detection of excipient in muirapuama dried extracts, generating no residue in the analysis. Scanning electron microscopy (SEM) images showed samples with a higher concentration of excipient, presenting better morphological characteristics and a lower moisture absorption rate. An eco-scale score value of 85 was achieved for UHPLC and 100 was achieved for NIR (excellent green analysis). Above all, these methods are rapid and green for the routine analysis of herbal medicines based on dried extracts.
Subject(s)
Green Chemistry Technology , Oleaceae/chemistry , Plant Extracts/analysis , Desiccation , Plant Extracts/chemistryABSTRACT
This work presents a new triptycene-based stationary phase (TP-PEG) combining the three-dimensional (3D) triptycene (TP) framework with polyethylene glycol (PEG) moieties for gas chromatographic (GC) separations. Its statically coated capillary column showed high column efficiency of 5263 plates/m determined by naphthalene at 120 °C. Its Rohrschneider-McReynolds constants and Abraham solvation system constants were measured to characterize its polarity and molecular interactions with analytes of different types. As evidenced, the TP-PEG column showed high-resolution performance for the isomers of anilines, phenols, halobenzenes and alkanes with distinct advantages over the PEG columns, particularly those critical isomers such as 3,5-/2,3-xylidine (R = 2.94), m-/p-chlorotoluene (R = 1.92), p-/m-cresol (R = 1.89), 2,2-dimethylbutane/2-methylpentane (R = 1.51), 2,2,3-trimethylbutane /2,3-dimethyl pentane (R = 1.74) and 2,3-dimethylpentane/n-heptane (R = 1.92). In addition, it exhibited good column repeatability and reproducibility with the relative standard deviation (RSD) values of 0.02%-0.09% for run-to-run, 0.13%-0.22% for day-to-day and 2.7%-4.1% for column-to-column, respectively, and a wide operational temperature range (30 °C-280 °C) . Its application to GC-MS analysis of the essential oil of Osmanthus fragrans has proven its good potential for practical analysis of complex samples.
Subject(s)
Anthracenes/chemistry , Gas Chromatography-Mass Spectrometry/methods , Oils, Volatile/chemistry , Oleaceae/chemistry , Polyethylene Glycols/chemistry , Alkanes/analysis , Alkanes/chemistry , Aniline Compounds/analysis , Aniline Compounds/chemistry , Benzene Derivatives/chemistry , Isomerism , Phenols/analysis , Phenols/chemistry , Reproducibility of ResultsABSTRACT
Although the antidiabetic efficacy of Nyctanthes arbor-tristis flowers has been reported, antiproliferative and anti-obesity activities are yet to be explored. We examined the anti-obesity and antiproliferative potentials of different fractions (hexane, chloroform, ethyl acetate, methanol) of N. abor-tristis flower extract for the first time using 3T3-L1 cells, primary peripheral blood mononuclear cells (PBMC) isolated from healthy and adult acute myeloid (AML) and chronic lymphocytic leukemia (CLL) patients, recombinant Jurkat T cells, and MCF7 cell lines. The in vitro hypoglycemic activity was evaluated using the inhibition of -amylase enzyme and glucose uptake by yeast cells. The percentage glucose uptake and -amylase inhibitory activity increased in a dose-dependent manner in the crude and the tested fractions (hexane and ethyl acetate). Inhibition of the 3T3-L1 cells' differentiation was observed in the ethyl acetate and chloroform fractions, followed by the hexane fraction. Antiproliferative analyses revealed that Nyctanthes exerted a high specific activity against anti-AML and anti-CLL PBMC cells, especially by the hexane and ethyl acetate fractions. The gas chromatography/mass spectrometry analysis indicated the presence of 1-heptacosanol (hexane fraction), 1-octadecene (hexane and chloroform fractions), and other organic compounds. Molecular docking demonstrated that phenol,2,5-bis(1,1-dimethylethyl) and 4-hydroxypyridine 1-oxide compounds showed specificity toward survivin protein, indicating the feasibility of N. abor-tristis in developing new drug leads against leukemia.
Subject(s)
Adipocytes/cytology , Antineoplastic Agents, Phytogenic/pharmacology , Flowers/chemistry , Leukemia, Lymphocytic, Chronic, B-Cell/metabolism , Leukemia, Myeloid, Acute/metabolism , Oleaceae/chemistry , Survivin/metabolism , 3T3-L1 Cells , Alkenes/chemistry , Animals , Cell Proliferation , Drug Evaluation, Preclinical , Gas Chromatography-Mass Spectrometry , Humans , Inhibitory Concentration 50 , Jurkat Cells , Leukemia, Lymphocytic, Chronic, B-Cell/drug therapy , Leukemia, Myeloid, Acute/drug therapy , Leukocytes, Mononuclear/cytology , MCF-7 Cells , Mice , Molecular Docking Simulation , Obesity/drug therapy , Plant Extracts/pharmacologyABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE: Myxopyrum serratulum A. W. Hill. (Oleaceae) is a traditionally used Indian medicinal plant for the treatment of cough, asthma and many other inflammatory diseases. AIM OF THE STUDY: In this study, the protective effects of M. serratulum on airway inflammation was investigated in ovalbumin (OVA)-induced murine model of allergic asthma and lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 murine macrophages, and the possible mechanisms were elucidated. MATERIALS AND METHODS: The phytochemicals present in the methanolic leaf extract of M. serratulum (MEMS) were identified by reverse phase high performance liquid chromatography (RP-HPLC) analysis. In vitro anti-inflammatory activity of MEMS were evaluated by estimating the levels of nitric oxide (NO), reactive oxygen species (ROS) and cytokines (IL-1α, IL-1ß, IL-2, IL-4, IL-6, IL-10, IL-12, IL-17A, IFN-γ, TNF-α, G-CSF and GM-CSF) in LPS-stimulated RAW 264.7 macrophages. In vivo anti-asthmatic activity of MEMS was studied using OVA-induced murine model. Airway hyperresponsiveness (AHR), was measured; total and differential cell counts, eosinophil peroxidase (EPO), prostaglandin E2 (PGE2), NO, ROS, and cytokines (IL-4, IL-5 and IL-13), were estimated in bronchoalveolar lavage fluid (BALF). Serum total IgE level was measured; and the histopathological changes of lung tissues were observed. The expressions of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in lung tissue homogenates were detected by Western blot. RESULTS: The chromatographic analysis of MEMS identified the presence of gallic acid, protocatechuic acid, catechin, ellagic acid, rutin, p-coumaric acid, quercetin, naringenin and apigenin. MEMS (125 and 250⯵g/mL) dose-dependently reduced the levels of NO, ROS and pro-inflammatory cytokines in LPS-stimulated RAW 264.7 macrophages. MEMS (200 and 400â¯mg/kg, p.o.) significantly (pâ¯<â¯0.05) alleviated AHR; number of inflammatory cells, EPO, PGE2, NO, ROS, and cytokines (IL-4, IL-5 and IL-13) in BALF; serum total IgE and the histopathological changes associated with lung inflammation. Western blot studies showed that MEMS substantially suppressed COX-2 and iNOS protein expressions in the lung tissues of OVA-sensitized/challenged mice. CONCLUSIONS: The present study corroborates for the first time the ameliorative effects of MEMS on airway inflammation by reducing the levels of oxidative stress, pro-inflammatory cytokines and inhibiting COX-2, iNOS protein expressions, thereby validating the ethnopharmacological uses of M. serratulum.
Subject(s)
Anti-Asthmatic Agents/pharmacology , Anti-Inflammatory Agents/pharmacology , Asthma/prevention & control , Bronchial Hyperreactivity/prevention & control , Bronchoconstriction/drug effects , Lung/drug effects , Macrophages/drug effects , Oleaceae , Plant Extracts/pharmacology , Animals , Anti-Asthmatic Agents/isolation & purification , Anti-Inflammatory Agents/isolation & purification , Asthma/immunology , Asthma/metabolism , Asthma/physiopathology , Bronchial Hyperreactivity/immunology , Bronchial Hyperreactivity/metabolism , Bronchial Hyperreactivity/physiopathology , Cytokines/metabolism , Disease Models, Animal , Female , Inflammation Mediators/metabolism , Lipopolysaccharides/pharmacology , Lung/immunology , Lung/metabolism , Lung/physiopathology , Macrophages/metabolism , Mice , Mice, Inbred BALB C , Nitric Oxide/metabolism , Oleaceae/chemistry , Ovalbumin , Plant Extracts/isolation & purification , Plant Leaves , RAW 264.7 Cells , Reactive Oxygen Species/metabolismABSTRACT
Triterpenoids, as an important family of plant secondary metabolites, have important biological activities associated with health and disease prevention. In this work, we proposed a HPLC-MS method for profiling multiple groups of triterpenoid acids and triterpenoid esters differing only in one position of the hydroxyl or methyl group in O. fragrans fruits. A total of thirty-one compounds were identified, and twenty-seven components were discovered in O. fragrans fruits for the first time. The HPLC-MS profiling method was applied in the analysis of the triterpenoids of O. fragrans flowers, and the time courses of triterpenoids of O. fragrans fruits.
Subject(s)
Carboxylic Acids/analysis , Esters/analysis , Fruit/chemistry , Oleaceae/chemistry , Triterpenes/analysis , Carboxylic Acids/chemistry , Chromatography, High Pressure Liquid/methods , Esters/chemistry , Flowers/chemistry , Isomerism , Mass Spectrometry/methods , Triterpenes/chemistryABSTRACT
RATIONALE: Floral volatiles are commonly present only at trace amounts and can be degraded or lost during vapor collection, which is often challenging from the analytical standpoint. Osmanthus fragrans Lour. is a widely cultivated plant known for the highly distinct fragrance of its flowers. The identification of specific volatile organic compounds (VOCs) and molecular differentiation of O. fragrans without any chemical pretreatment and VOC collection are important. METHODS: Twenty-eight VOCs released by the flowers from ten different cultivars of O. fragrans were identified using neutral desorption extractive atmospheric pressure chemical ionization mass spectrometry (ND-EAPCI-MS) without any chemical pretreatment or VOC collection. Chemical identification was performed by high-resolution MSn analysis and whenever possible was confirmed by the analysis of standards. RESULTS: According to our literature search, nine of the identified VOCs, 3-buten-2-one, cyclohexadiene, 2-methylfuran, 3-allylcyclohexene, cuminyl alcohol, hotrienol oxide, epoxy-linalool oxide, N-(2-hydroxyethyl) octanamide, and 3-hydroxy-dihydro-ß-ionone, have not been reported in O. fragrans in earlier studies. Confident differentiation between ten different cultivars of O. fragrans was achieved by the principal component analysis of the mass spectrometric results. CONCLUSIONS: The results of our ND-EAPCI-MS analysis substantially increase our knowledge about the chemistry of the O. fragrans floral fragrance and demonstrate the power of this technique for direct molecular profiling for plant recognition or in biotechnological applications.
Subject(s)
Flowers/chemistry , Mass Spectrometry/methods , Oleaceae/chemistry , Volatile Organic Compounds/chemistry , Molecular Structure , Oleaceae/classificationABSTRACT
The dried flowers of Chionanthus retusus were extracted with 80% MeOH, and the concentrate was divided into EtOAc, n-BuOH, and H2O fractions. Repeated SiO2, octadecyl SiO2 (ODS), and Sephadex LH-20 column chromatography of the EtOAc fraction led to the isolation of four flavonols (1-4), three flavones (5-7), four flavanonols (8-11), and one flavanone (12), which were identified based on extensive analysis of various spectroscopic data. Flavonoids 4-6 and 8-11 were isolated from the flowers of C. retusus for the first time in this study. Flavonoids 1, 2, 5, 6, 8, and 10-12 significantly inhibited NO production in RAW 264.7 cells stimulated by lipopolysaccharide (LPS) and glutamate-induced cell toxicity and effectively increased HO-1 protein expression in mouse hippocampal HT22 cells. Flavonoids with significant neuroprotective activity were also found to recover oxidative-stress-induced cell damage by increasing HO-1 protein expression. This article demonstrates that flavonoids from C. retusus flowers have significant potential as therapeutic materials in inflammation and neurodisease.
Subject(s)
Flavonoids/pharmacology , Flowers/chemistry , Glutamic Acid/toxicity , Oleaceae/chemistry , Animals , Cell Line, Tumor , Cell Survival/drug effects , Heme Oxygenase-1/metabolism , Lipopolysaccharides/pharmacology , Mice , RAW 264.7 Cells , Signal Transduction/drug effectsABSTRACT
In this work, dried flowers of Osmanthus fragrans Lour. were applied as green precursors to synthesize carbon dots (CDs) by a green hydrothermal method for the first time. The CDs showed strong blue fluorescence at 410 nm under 340-nm excitation with a quantum yield of approximately 18.53%. Furthermore, the CDs were applied for the sensitive detection of Fe3+. The linear response of Fe3+ ranged from 10 nM to 50 µM with a limit of detection as low as 5 nM. In addition, other ions were used as competitive substances to explore the selectivity of CDs for Fe3+. The fluorescence quenching effect of Fe3+ was much stronger, which demonstrated that the CDs had high selectivity for Fe3+ and they can be employed for the selective detection of Fe3+. The potential fluorescence quenching mechanism between CDs and Fe3+ was identified as the inner filter effect. The CDs were then used as a fluorescent sensor for the detection of Fe3+ in water samples and human serum; the recovery range was 93.76-113.80% (relative standard deviation less than 0.79%). These results indicate that the CDs can be applied for the sensitive and selective detection of Fe3+ in real samples. Moreover, on the basis of the redox reaction between Fe3+ and ascorbic acid (AA), the CD-Fe3+ system can be used as a fluorescent "off-on" sensor for the detection of AA with a limit of detection of 5 µM. What is more, because of their low toxicity and biocompatibility, the CDs can also be used for cell imaging and acted as a fluorescent probe for fluorescence imaging of Fe3+ and AA in living cells. These results demonstrate that the CDs have great potential for application in the fields of sensing, bioimaging, and even disease diagnosis.
Subject(s)
Ascorbic Acid/analysis , Carbon/chemistry , Ferric Compounds/analysis , Flowers/chemistry , Green Chemistry Technology/methods , Nanoparticles/chemistry , Oleaceae/chemistry , A549 Cells , Culture Media , Humans , Limit of Detection , Microscopy, Electron, Transmission , Photoelectron Spectroscopy , Spectrometry, FluorescenceABSTRACT
In the quest to search and discover bioactive compounds from nature, terpenoids have emerged as one of the most interesting and researched classes of compounds. Secoiridoid, a type of the terpenoid, has also been extensively studied, especially their chemical structures and pharmacological effects. Oleaceae is a family of woody dicotyledonous plants with broad economic and medicinal values. This family contains a large number of flavonoids, monoterpenoids, iridoids, secoiridoids and phenylethyl alcohols, of which the secoiridoids have various biological activities. The purpose of this review is to summarize the phytochemical and pharmacological of the secoiridoids (glycosides, aglycones, derivatives and dimers) in the Oleaceae family from 1987 to 2018. This review will also serve as a reference for further studies.