ABSTRACT
Environmental contaminants have been suggested as a possible cause of fibropapillomatosis (FP) in green sea turtles. In turn, a reduced concentration of serum cholesterol has been indicated as a reliable biomarker of malignancy in vertebrates, including marine turtles. In the present study, metal (Ag, Cd, Cu, Fe, Ni, Pb and Zn) concentrations, oxidative stress parameters [antioxidant capacity against peroxyl radicals (ACAP), protein carbonyls (PC), lipid peroxidation (LPO), frequency of micronucleated cells (FMC)], water content, cholesterol concentration and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) activity were analyzed in the blood/serum of juvenile (29.3-59.5cm) female green sea turtles (Chelonia mydas) with FP (n=14) and without FP (n=13) sampled at Ubatuba coast (São Paulo State, southeastern Brazil). Green sea turtles were grouped and analyzed according to the severity of tumors. Individuals heavily afflicted with FP showed significantly higher blood Cu, Pb and Fe concentrations, blood LPO levels, as well as significantly lower serum cholesterol concentrations and HMGR activity than turtles without FP. Significant and positive correlations were observed between HMGR activity and cholesterol concentrations, as well as LPO levels and Fe and Pb concentrations. In turn, Cu and Pb concentrations were significantly and negatively correlated with HMGR activity and cholesterol concentration. Furthermore, Cu, Fe and Pb were positively correlated with each other. Therefore, the reduced concentration of serum cholesterol observed in green sea turtles heavily afflicted with FP is related to a Cu- and Pb-induced inhibition of HMGR activity paralleled by a higher LPO rate induced by increased Fe and Pb concentrations. As oxidative stress is implicated in the pathogenesis of viral infections, our findings support the idea that metal contamination, especially by Cu, Fe and Pb, may be implicated in the etiology of FP in green sea turtles through oxidative stress generation.
Subject(s)
Metals/toxicity , Turtles/metabolism , Water Pollutants, Chemical/toxicity , Animals , Atlantic Ocean , Biomarkers/metabolism , Body Size/drug effects , Brazil , Cholesterol/blood , Female , Lipid Peroxidation/drug effects , Metals/blood , Papilloma/blood , Papilloma/etiology , Turtles/bloodABSTRACT
Confluent and reticulated papillomatosis of Gougerot and Carteaud is a dermatosis that despite showing characteristic clinical signs is often poorly recognized and diagnosed. The authors present a case with extensive skin involvement, discuss its association with obesity and describe dermoscopic findings making the histopathological correlation.
Subject(s)
Obesity/complications , Papilloma/pathology , Skin Neoplasms/pathology , Adult , Body Mass Index , Dermoscopy , Humans , Male , Obesity/therapy , Papilloma/etiology , Papilloma/therapy , Skin Neoplasms/etiology , Skin Neoplasms/therapy , Treatment Outcome , Weight LossABSTRACT
Confluent and reticulated papillomatosis of Gougerot and Carteaud is a dermatosis that despite showing characteristic clinical signs is often poorly recognized and diagnosed. The authors present a case with extensive skin involvement, discuss its association with obesity and describe dermoscopic findings making the histopathological correlation.
Subject(s)
Adult , Humans , Male , Obesity/complications , Papilloma/pathology , Skin Neoplasms/pathology , Body Mass Index , Dermoscopy , Obesity/therapy , Papilloma/etiology , Papilloma/therapy , Skin Neoplasms/etiology , Skin Neoplasms/therapy , Treatment Outcome , Weight LossABSTRACT
La papilomatosis reticulada y confluente (PRC) es una rara dermatosis de etiología desconocida, que se caracteriza por placas hiperpigmentadas que confluyen en el centro y presentan patrón reticulado en la periferia. Es subdiagnosticada y no tiene tratamiento específico. Se reporta el caso de una paciente mujer de 36 años con papilomatosis reticulada y confluente en zona intermamaria. Se realiza revisión de la literatura.
Confluent and reticulated papillomatosis is a rare dermatosis of unknown etiology, characterized by hyperpigmented plaques that meet in the center and reticulated pattern present in the periphery. Is underdiagnosed and no specific treatment. We report the case of a woman aged 36 with confluent and reticulated papillomaiosis intermammary area. Literature review was performed.
Subject(s)
Humans , Adult , Female , Papilloma , Papilloma/etiology , Papilloma/history , Papilloma/pathology , Papilloma/therapyABSTRACT
La infección persistente por ciertos tipos de alto riesgo oncogénico de virus papiloma humano (VPHAR) es el principal factor de riesgo para el desarrollo de cáncer de cuello uterino y sus lesiones precursoras. Los VPHAR inducen alteraciones moleculares durante todo el proceso de carcinogénesis cervical, que provocan la acumulación de errores genéticos, con la consecuente inestabilidad genética y transformación maligna. Estas alteraciones son producidas por la acción directa de las oncoproteínas virales E6 y E7 sobre las principales proteínas celulares supresoras de tumor, p53 y pRb, respectivamente, y pueden ser monitoreadas durante el surgimiento de la lesión neoplásica, mediante el uso de biomarcadores. En este artículo se revisan las últimas tendencias sobre el uso del estudio inmunocitoquímico, como una prueba complementaria a la citología y a la detección y tipificación de VPHAR en la evaluación de la expresión de biomarcadores como la proteína inhibidora de la proliferación celular p16INK4a, marcador único o combinada con otros biomarcadores, que puedan contribuir eficazmente en la detección de las pacientes con mayor riesgo a desarrollar neoplasia del cuello uterino asociada a la infección por VPHAR, durante la pesquisa de cáncer de cuello uterino de rutina y en el manejo clínico adecuado y oportuno.
Persistent infection with certain types of high oncogenic risk human papillomavirus (HR-HPV) is the main risk factor for developing cervical cancer and its precursor lesions. HR-HPV induces molecular changes during cervical carcinogenesis, causing the accumulation of genetic anomalies, with subsequent genetic instability and malignant transformation. These alterations are produced by the direct action of the E6 and E7 viral oncoproteins on principal tumor cell suppressor proteins, p53 and pRb, respectively, and can be monitored during growth of the neoplastic lesion using biomarkers. In this paper we review the latest trends on the use of immunocytochemistry as a complementary test to cytology and HR-HPV detection and typing in evaluating expression of biomarkers such as the p16INK4a cell proliferation inhibitor protein, as a single marker or combined with other biomarkers, which can contribute effectively to the detection of patients with increased risk of developing cervical neoplasia associated with HR-HPV infection during routine screening for cervical cancer and in appropriate clinical management.
Subject(s)
Humans , Adult , Female , Young Adult , Biomarkers, Pharmacological/analysis , Biomarkers, Pharmacological/blood , Epithelial Cells/chemistry , Uterine Cervical Neoplasms/diagnosis , Uterine Cervical Neoplasms/etiology , Uterine Cervical Neoplasms/pathology , Papilloma/etiology , Papilloma/chemistry , Papilloma/blood , Blood Chemical Analysis , Hematology , Immunohistochemistry , Medical OncologyABSTRACT
Introducción: los condilomas acuminados están entre las ITS más frecuentes y afectan hasta el 1, 7% de la población general. Objetivos: caracterizar un grupo de pacientes con condilomas acuminados atendidos en la consulta de patología del tractus genital inferior. Método: se realizó una intervención cuasiexperimental en pacientes con condilomas acuminados en el Hospital Materno Mariana Grajales en los años 2008 y 2009, se tomó como muestra para este estudio a 382 individuos de ambos sexos...
Subject(s)
Female , Condylomata Acuminata , Sexually Transmitted Diseases/diagnosis , Papilloma/etiologyABSTRACT
Due to the observation of a great number of patients having achrocordons, when they underwent fine needle biopsies for thyroid nodules, we decided to perform a prospective study to investigate the relationship between this finding and the presence of insulin resistance (IR), since achrocordons are commonly seen in hyperinsulinemic subjects. A total of 120 consecutive women, aged 18-35 yrs were studied. All subjects were also evaluated by thyroid ultrasound (US) for measuring thyroid volume and the presence of non-palpable nodules. Basal and post-prandial serum insulin was measured in all of them, as well as the Homeostasis Model Assessment (HOMA). Subjects were divided in two groups: Group A, with achrocordons (n = 44) and Group B, without achrocordons (n = 76). Group A showed 24 patients (54.5%) with thyroid nodules, whereas Group B only 13 subjects (17.1%); p = 0.0087. When we considered, as having high normal thyroid volume, the glands weighting more than 16 grams by US, without nodules, it was found that 8/44 cases from Group A (18.6%) and 3/76 from Group B (3.9%) fitted in such category, p = 0.0076. In patients with nodules and/or bigger thyroids, IR was observed in 36/44 (81.8%) of Group A and 14/76 (18.4%) of Group B, p = 0.0069, while the overall prevalence of IR was 0.47 in Group A and 0.05 in Group B, p = 0.00094. It is concluded that patients with achrocordons have a higher prevalence of US-detected thyroid nodules and larger thyroid glands. Then, it may be beneficial to search for thyroid abnormalities in those subjects with skin tags.
Subject(s)
Insulin Resistance , Papilloma/etiology , Skin Neoplasms/etiology , Thyroid Nodule/complications , Adolescent , Adult , Case-Control Studies , Female , Homeostasis , Humans , Papilloma/pathology , Prevalence , Prospective Studies , Skin Neoplasms/pathology , Thyroid Nodule/diagnostic imaging , Ultrasonography , Young AdultABSTRACT
Due to the observation of a great number of patients having achrocordons, when they underwent fine needle biopsies for thyroid nodules, we decided to perform a prospective study to investigate the relationship between this finding and the presence of insulin resistance (IR), since achrocordons are commonly seen in hyperinsulinemic subjects. A total of 120 consecutive women, aged 18-35 yrs were studied. All subjects were also evaluated by thyroid ultrasound (US) for measuring thyroid volume and the presence of nonpalpable nodules. Basal and post-prandial serum insulin was measured in all of them, as well as the Homeostasis Model Assessment (HOMA). Subjects were divided in two groups: Group A, with achrocordons (n = 44) and Group B, without achrocordons (n = 76). Group A showed 24 patients (54.5%) with thyroid nodules, whereas Group B only 13 subjects (17.1%); p = 0.0087. When we considered, as having high normal thyroid volume, the glands weighting more than 16 grams by US, without nodules, it was found that 8/44 cases from Group A (18.6%) and 3/76 from Group B (3.9%) fitted in such category, p = 0.0076. In patients with nodules and/or bigger thyroids, IR was observed in 36/44 (81.8%) of Group A and 14/76 (18.4%) of Group B, p = 0.0069, while the overall prevalence of IR was 0.47 in Group A and 0.05 in Group B, p = 0.00094. It is concluded that patients with achrocordons have a higher prevalence of US-detected thyroid nodules and larger thyroid glands. Then, it may be beneficial to search for thyroid abnormalities in those subjects with skin tags.
Debido a la alta frecuencia de acrocordones en pacientes que concurrían a nuestro servicio para realizar punciones aspirativas de nódulos tiroideos, realizamos un estudio prospectivo para investigar la relación entre este hallazgo y la presencia de insulino resistencia (IR), dado que los acrocordones son frecuentemente observados en pacientes hiperinsulinémicos. Se incluyeron 120 pacientes consecutivas, con edades entre 18 y 35 años. Todas fueron evaluadas con una ecografía tiroidea para determinar su volumen y la presencia de nódulos no palpables. Se midió insulinemia basal y post prandial, así como el índice Homeostasis Model Assessment (HOMA). Las pacientes se dividieron en 2 grupos: Grupo A, con acrocordones (n = 44) y Grupo B, sin acrocordones (n = 76). En el Grupo A se encontraron 24 (54.5%) con nódulos tiroideos, mientras que el Grupo B, sólo 13 (17.1%); p = 0.0087. Cuando consideramos la presencia de una glándula tiroides de tamaño elevado pero dentro de los límites normales, medida por ecografía (peso mayor a 16 gramos sin nódulos), encontramos que 8/44 casos del Grupo A (18.6%) y 3/76 del Grupo B (3.9%) entraron en esta categoría, p = 0.0076. En aquellas pacientes con nódulos tiroideos o glándula tiroides de mayor tamaño, observamos IR en 36/44 (81.8%) del Grupo A y en 14/76 (18.4%) del Grupo B, p = 0.0069, mientras que la prevalencia total de IR fue del 0.47 en el Grupo A y del 0.05 en el Grupo B, p = 0.00094. En conclusión, las pacientes con acrocordones tuvieron mayor prevalencia de nódulos tiroideos detectados por ecografía, glándula tiroides de mayor tamaño y mayor proporción de insulino resistencia.
Subject(s)
Adolescent , Adult , Female , Humans , Young Adult , Insulin Resistance , Papilloma/etiology , Skin Neoplasms/etiology , Thyroid Nodule/complications , Case-Control Studies , Homeostasis , Prevalence , Prospective Studies , Papilloma/pathology , Skin Neoplasms/pathology , Thyroid NoduleSubject(s)
Middle Aged , Cyclophosphamide/therapeutic use , Dermatitis, Exfoliative/complications , Dermatitis, Exfoliative/pathology , Dermatitis, Exfoliative/therapy , Epidermolysis Bullosa Dystrophica/pathology , Steroids/therapeutic use , Parakeratosis/etiology , Pruritus/etiology , Cheilitis/diagnosis , Acanthosis Nigricans , Biopsy/methods , Papilloma/etiologyABSTRACT
OBJECTIVE: To establish the association between high-grade intraepithelial lesions and cervical-uterine-cancer, and the infection by human papillomavirus, genetic antecedents, socioeconomics, sexual behavior and gynecology and obstetrics factors in women of the State of Nayarit, Mexico. MATERIALS AND METHODS: With a case-control design were studied 66 cases of high-grade intraepithelial lesions and cervical-uterine-cancer, and 132 controls. The information upon the risk factors was obtained by the application of a structured questionnaire. Polymerase Chain Reaction executed the virus identification. In the statistical analysis the association was obtained by odds ratio. The statistical significance was evaluated by the chi-square-Fisher and Student t tests, and multivariate logistic regression was used to explain the factors' influence. RESULTS: In women with high-risk squamous intraepithelial lesions and cervical-uterine-cancer, the most frequently high-risk human papillomavirus found were: 18, 35, 58, 16, 31, 33 and 51. CONCLUSIONS: Familial data of cervical-uterine-cancer, socioeconomic level, number of sexual partners, data of sexual transmitted diseases, and infection due to human papillomavirus 18 and 35 are the factors related to high-risk squamous intraepithelial lesions and cervical-uterine-cancer.
Subject(s)
Papilloma/epidemiology , Papilloma/etiology , Uterine Cervical Neoplasms/epidemiology , Uterine Cervical Neoplasms/etiology , Uterine Neoplasms/epidemiology , Uterine Neoplasms/etiology , Adult , Aged , Catchment Area, Health , Female , Humans , Mexico/epidemiology , Middle Aged , Risk FactorsABSTRACT
La papilomatosis respiratoria recidivante es una enfermedad de la vía aérea que afecta a las personas de todas las edades y que se caracteriza por la aparición de masas epiteliales recidivantes y benignas. Su evolución es multiforme y va desde su desaparición luego de un período de tratamiento relativamente corto y sencillo hasta su progresión por toda la vía aérea, llegando a sitios inaccesibles para el cirujano, obstruyéndola y a veces transformándose en un carcinoma pavimentosos que puede llevar al paciente a la muerte.
Subject(s)
Humans , Laryngeal Neoplasms/epidemiology , Laryngeal Neoplasms/therapy , Papilloma/epidemiology , Papilloma/therapy , Chemotherapy, Adjuvant , Immunization , Laryngeal Neoplasms/surgery , Laryngeal Neoplasms/etiology , Laryngeal Neoplasms/drug therapy , Papilloma/surgery , Papilloma/etiology , Papilloma/drug therapyABSTRACT
The role of human papillomaviruses (HPV) in laryngeal squamous cell carcinoma has not yet been established. Thirty-three cases of laryngeal squamous cell carcinoma were analysed for the presence of HPV DNA and compared with 25 cases of normal larynx and 29 cases of laryngeal squamous papilloma in their positivity index. The presence of HPV DNA was analysed by using L1 consensus primers and also by primers specific for the E7 gene of HPV types 16 and 18. Four normal laryngeal samples (16%) were positive for HPV DNA against the 24 samples (82%) (p < 0.001) found for laryngeal papilloma and 16 (48.5%) (p < 0.05) found for laryngeal squamous cell carcinoma. HPV 16 was the type most frequently found in laryngeal carcinoma samples. Our results support an etiologic role for this type of HPV in the pathogenesis of laryngeal carcinoma.
Subject(s)
Carcinoma, Squamous Cell/virology , DNA, Viral/genetics , Laryngeal Neoplasms/virology , Papilloma/virology , Papillomaviridae/genetics , Papillomavirus Infections/virology , Tumor Virus Infections/virology , Adult , Aged , Aged, 80 and over , Base Sequence , Biopsy , Blotting, Southern/methods , Blotting, Southern/statistics & numerical data , Carcinoma, Squamous Cell/etiology , Chi-Square Distribution , DNA Primers , DNA, Viral/analysis , Epithelium/pathology , Epithelium/virology , Humans , Laryngeal Neoplasms/etiology , Larynx/pathology , Larynx/virology , Middle Aged , Molecular Sequence Data , Papilloma/etiology , Papillomavirus Infections/etiology , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/statistics & numerical data , Tumor Virus Infections/etiologyABSTRACT
We describe 8 patients affected with Costello syndrome including an affected sib pair and review the literature on 29 previously reported cases. We emphasize an association with advanced parental age, which is consistent with autosomal dominant inheritance with germline mosaicism. The pathogenesis appears to involve metabolic dysfunction, with growth disturbance, storage disorder appearance, acanthosis nigricans, hypertrophic cardiomyopathy, and occasional abnormalities of glucose metabolism. Although the cause is currently unknown, Costello syndrome is interesting because of a potential genetic-metabolic etiology.
Subject(s)
Dwarfism/pathology , Facies , Intellectual Disability/pathology , Acanthosis Nigricans/pathology , Acanthosis Nigricans/physiopathology , Adolescent , Adult , Age Factors , Carbohydrate Metabolism, Inborn Errors/pathology , Carbohydrate Metabolism, Inborn Errors/physiopathology , Cardiomyopathy, Hypertrophic/pathology , Cardiomyopathy, Hypertrophic/physiopathology , Child , Child, Preschool , Diagnosis, Differential , Dwarfism/diagnosis , Dwarfism/etiology , Dwarfism/genetics , Dwarfism/physiopathology , Female , Genes, Dominant/genetics , Germ-Line Mutation/genetics , Glucose/metabolism , Humans , Intellectual Disability/diagnosis , Intellectual Disability/etiology , Intellectual Disability/genetics , Intellectual Disability/physiopathology , Male , Metabolic Diseases/pathology , Metabolic Diseases/physiopathology , Mosaicism/genetics , Nose Neoplasms/diagnosis , Nose Neoplasms/etiology , Nose Neoplasms/genetics , Nose Neoplasms/pathology , Nose Neoplasms/physiopathology , Papilloma/diagnosis , Papilloma/etiology , Papilloma/genetics , Papilloma/pathology , Papilloma/physiopathology , Parents , Phenotype , SyndromeABSTRACT
Se analizaron 30 pacientes en cuyas parejas se habían diagnosticado lesiones de PVH por colposcopía y biopsia. A todos se les sometió a una exploración y a una colposcopía con ácido acético. Se encontró que el 96 por ciento tenían lesiones condilomatosas. Al aplicar el tratamiento en base a 5 fluoracilo y aciclovir, 92 por ciento mostraron curación, hubo una tasa de 8 por ciento de complicaciones todas derivadas de una mala aplicación del producto. Se concluye que el 5 fluoracilo y el Aciclovir, administrado acorde al protocolo utilizado resultó útil en el 92 por ciento de la serie
Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Papilloma/diagnosis , Papilloma/etiology , Papilloma/therapy , Acyclovir/therapeutic use , Men , Costa RicaABSTRACT
O carcinoma da vulva representa cerca de 2 por cento a 5 por cento de todas as neoplasias genitais malignas da mulher. O Brasil apresenta na publicacao da Agencia Internacional para Pesquisa em Cancer (IACR) a primeira e a segunda maiores incidencias mundiais deste tumor entre os dados de 40 paises. Apesar de ser uma doenca que predomina em mulheres idosas, recentemente tem sido relatado um aumento de sua incidencia em mulheres jovens. Os autores discutem os diversos fatores de risco para o desenvolvimento do carcinoma vulvar
Subject(s)
Humans , Female , Condylomata Acuminata/diagnosis , Papilloma/etiology , Vulvar Neoplasms/diagnosis , Carcinoma , Risk Factors , Uterus/pathology , Vulvar Neoplasms/etiologyABSTRACT
Before the notorious increase of congenital malformations among individuals belonging to rurality, which are faced to yield between watering by sprinklers with weed-killers and the alarming growth of cardiovascular and pre--and malignant diseases we decided to undertake a perusal among the agents regarded since ever as bias toward to those pathologies. Following that conception we found out a common tendency between malnutrition, diabetes, alcoholism, drug-addiction, weed-killers, and so on. The whole group generate energy reduction (ATP) starting from which appears a biochemical complex competent to give way to any cardiovascular pathology, autoimmune maladies or even congenital malformations.
Subject(s)
Dermatomyositis/etiology , Down Syndrome/etiology , Esophageal Achalasia/etiology , Papilloma/etiology , Vocal Cords , Child , Dermatomyositis/complications , Dermatomyositis/diagnosis , Down Syndrome/complications , Esophageal Achalasia/complications , Esophageal Achalasia/therapy , Female , Humans , Male , Papilloma/complications , Papilloma/therapyABSTRACT
249 sueros de pacientes con diagnóstico de Carcinoma cervical (CA INV), Neoplasia Intraepitelial Cervical (NIC) y de mujeres sanas fueron analizados mediante la técnica de ELISA empleando 14 icosapéptidos sintéticos sobrelapados que representan la secuencia de aminoácidos de la proteína E7 del virus del papiloma tipo 16 (E7-VPH 16).Las principales regiones de la proteína reconocidas por las pacientes con diagnóstico de CA: invasivo fueron representadas por los péptidos E7 (1-20) (11.3 por ciento) y E7 (66-85 ) (12.5 por ciento) Ninguno de los controles mostró reactividad hacia estos péptidos. Para confirmar esta reactividad, los sueros fueron analizados mediante inmunotransferencia con proteína E7-VPH 16 recombinante; 28.2 por ciento de los sueros del grupo de CA invasivo fueron positivos, ningún suero del grupo control reconoció la proteína recombinante. Las reactividades de sueros de pacientes y controles hacia péptidos diferentes de E7 (11-30) y E7 (66-85) no fueron confirmados. La detección de anticuerpos hacia estos dos péptidos podría ser útil en el seguimiento de las pacientes con CA de cérvix
Subject(s)
Humans , Female , Adult , Papilloma/complications , Papilloma/etiology , Papilloma/prevention & control , Papilloma/therapy , Papilloma/virologyABSTRACT
The A. inform about the results achieved with lysozyme chlorhydrate in the treatment of 15 patients with juvenile laryngeal papillomatosis. The lysozyme is an electropositive enzyme which synthesis is related to the degree of proteins and vitamin B complex ingestion. Lysozyme is a component of the immunitary inespecific system, serving to prevent against HPV-DNA at the level of the secretory film of the mucociliary apparatus of the respiratory mucous membrane. Furthermore, lysozyme hydrolyzes the mucopolysaccharide of the connective tissue and inhibits the virus-DNA replication. 100-300 mgr daily during 30-60 days simultaneously with hyperproteic diet and vitamin B complex (after correction of the nutrimental deficiencies) brought about the evanishment of papillomatosis. The A. suggest that the predisposition to infection by virus DNA is primarily of immunitary origin, because of lysozyme deficiency, and secondary due to a low intake of proteins and vitamin B complex.