ABSTRACT
Patients with pathological nipple discharge (PND) often undergo local surgical procedures because standard radiologic imaging fails to identify the underlying cause. MicroRNA (MiRNA) expression analysis of nipple fluid holds potential for distinguishing between breast diseases. This study aimed to compare miRNA expression levels between nipple fluids from patients with PND to identify possible relevant miRNAs that could differentiate between intraductal papillomas and no abnormalities in the breast tissue. Nipple fluid samples from patients with PND without radiological and pathological suspicion for malignancy who underwent a ductoscopy procedure were analyzed. We used univariate and multivariate regression analyses to identify nipple fluid miRNAs differing between pathologically confirmed papillomas and breast tissue without abnormalities. A total of 27 nipple fluid samples from patients with PND were included for miRNA expression analysis. Out of the 22 miRNAs examined, only miR-145-5p was significantly differentially expressed (upregulated) in nipple fluid from patients with an intraductal papilloma compared to patients showing no breast abnormalities (OR 4.76, p = 0.046), with a diagnostic accuracy of 92%. miR-145-5p expression in nipple fluid differs for intraductal papillomas and breast tissue without abnormalities and, therefore, has potential as a diagnostic marker to signal presence of papillomas in PND patients. However, further refinement and validation in clinical trials are necessary to establish its clinical applicability.
Subject(s)
Breast Diseases , Breast Neoplasms , MicroRNAs , Nipple Discharge , Papilloma, Intraductal , Papilloma , Humans , Female , Papilloma, Intraductal/diagnosis , Papilloma, Intraductal/genetics , Papilloma, Intraductal/pathology , Endoscopy/methods , Nipple Discharge/metabolism , Breast Neoplasms/diagnosis , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Breast Diseases/metabolism , Nipples/metabolism , MicroRNAs/genetics , MicroRNAs/metabolism , Papilloma/diagnosis , Papilloma/genetics , Papilloma/metabolismABSTRACT
Cervical cancer (CC) is the second most common cancer worldwide, strongly linked to high-risk human papilloma virus infection. Although screening programs have led to a relevant reduction in the incidence and mortality due to CC in developed countries, it is still an important cause of mortality in undeveloped countries. Clinical stage is still the most relevant prognostic factor. In early stages, the primary treatment is surgery or radiotherapy, whereas concomitant chemo-radiotherapy is the conventional approach in locally advanced stages. In the setting of recurrent or metastatic CC, for the first time ever, the combination of chemotherapy plus bevacizumab prolongs the overall survival beyond 12 months. Therefore, this regimen is considered by most of the oncologist a new standard of care for metastatic/recurrent CC (AU)
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Subject(s)
Humans , Male , Female , /standards , Uterine Cervical Neoplasms/metabolism , Uterine Cervical Neoplasms/pathology , Papilloma/diagnosis , Papilloma/metabolism , Adenocarcinoma/metabolism , Lymph Nodes/metabolism , Therapeutics/instrumentation , Therapeutics/methods , Uterine Cervical Neoplasms/drug therapy , Uterine Cervical Neoplasms/therapy , Papilloma/complications , Papilloma/pathology , Adenocarcinoma/complications , Lymph Nodes/abnormalities , Therapeutics/standards , TherapeuticsABSTRACT
Objetivo: Estudio descriptivo de pacientes del Hospital Universitario de La Princesa con diagnóstico de tumoración endobronquial benigna. Material y métodos: Se analizan las características de pacientes del Servicio de Cirugía Torácica del Hospital Universitario de La Princesa desde el año 2002 a 2012. Resultados: Presentamos 9 casos diagnosticados, 8 de ellos tratados mediante broncoscopia láser y uno mediante cirugía abierta. El láser fue aplicado a través de broncoscopia flexible y broncoscopia rígida. La mayoría fueron tratados con una única sesión, siendo dados de alta el mismo día del procedimiento sin complicaciones. A los 2 años de seguimiento se objetiva una remisión del 100%. Conclusiones: Consideramos que el tratamiento deber ser individualizado, teniendo en cuenta como terapia de primera línea la aplicación de láser endoscópico
Aims: A descriptive study of patients of the La Princesa University Hospital diagnosed with benign endobronchial tumor. Material and methods: The characteristics of patients admitted to the La Princesa University Hospital - Thoracic Surgery Unit during 2002-2012 were analyzed. Results: We present 9 diagnosed cases, 8 of them treated by laser bronchoscopy and one open surgery. The laser was applied via flexible bronchoscopy, rigid bronchoscopy. Most were treated with one session being discharged the same day of the procedure without complications. At 2 years of follow remission of 100% objective. Conclusions: We believe that treatment should be individualized, taking into account as first-line therapy application of endoscopic laser
Subject(s)
Female , Humans , Male , Laser Therapy/methods , Laser Therapy/standards , Bronchial Neoplasms/metabolism , Bronchial Neoplasms/pathology , Lipoma/pathology , Hematoma/blood , Hematoma/metabolism , Ambulatory Surgical Procedures/methods , Papilloma/diagnosis , Laser Therapy/trends , Laser Therapy , Bronchial Neoplasms/complications , Lipoma/genetics , Hematoma/complications , Hematoma/pathology , Ambulatory Surgical Procedures/standards , Papilloma/metabolismABSTRACT
OBJETIVO: Presentar el caso clínico de una paciente con disminución psíquica importante que es diagnosticada de un tumor uretral y su evolución. MÉTODO/RESULTADO: La paciente se intervenida quirúrgicamente realizándose el estudio anatomopatológico. Se diagnostica de adenocarcinoma uretral. La paciente está libre de enfermedad a los 12 meses de la cirugía. CONCLUSIONES: Se realiza una revisión del diagnóstico y tratamiento de dicha patología (AU)
OBJECTIVE: To report the case of a urethral tumour in a patient with mental deficiency. METHODS/ RESULTS: Complete resection of the tumour was performed and the pathologic examination informed the presence of urethral adenocarcinoma. The patient is disease-free twelve months after surgery. CONCLUSIONS: Bibliographic review for diagnosis and treatment was performed (AU)
Subject(s)
Humans , Female , Adult , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Intellectual Disability , Gynecology/education , Epilepsy/complications , Epilepsy/pathology , Ultrasonography/instrumentation , Papilloma/metabolism , Sexually Transmitted Diseases/transmission , Sexually Transmitted Diseases/virology , Adenocarcinoma/drug therapy , Adenocarcinoma/radiotherapy , Gynecology/methods , Epilepsy/diagnosis , Epilepsy/metabolism , Ultrasonography/standards , Papilloma/complications , Sexually Transmitted Diseases/metabolism , Sexually Transmitted Diseases/prevention & controlABSTRACT
Introducción: La detección de la sobreexpresión de la proteína p16 mediante técnicas inmunohistoquímicas e inmunocitoquímicas es un valor seguro en la identificación de lesiones epiteliales cervicales de alto grado, sirviendo al mismo tiempo para detectar aquellas lesiones de bajo grado cito o histológico con integración viral, firmes candidatas a la progresión a lesión de alto grado. Material y métodos: Hemos evaluado la eficacia de la detección inmunohistoquímica e inmunocitoquímica de la sobreexpresión de la proteína p16 sobre muestras de cérvix uterino. Para ello hemos recurrido a 58 casos de biopsias de cérvix y 53 citologías con diagnóstico de positividad para la infección por el virus del papiloma humano. Resultados: Hemos observado cómo el 100% de las lesiones de alto grado eran teñidas mientras que so ́lo un porcentaje de las de bajo grado hacían lo propio. Los resultados obtenidos en citología fueron extrapolados a las citologías (correspondientes a las mismas pacientes que las biopsias) obteniendo similares resultados. Conclusiones: La identificación de la sobreexpresión de la proteína p16 por métodos inmunocitoquímicos sobre citologías cervicovaginales convencionales muestra resultados superponibles a los obtenidos sobre muestras histológicas (AU)
Introduction: Detection of p16 expression by immunohistochemistry and immunocytochemistry is a good standard for the identification of high-grade cervical epithelial lesions and low-grade lesions with DNA HPV viral integration (with a tendency for progression). Material and methods: We evaluated p16 expression in 58 HPV-positive cervical biopsies and 53 conventional cytological samples that tested HPV-positive with immunohistochemical and immunocytochemical techniques. Results: All high-grade lesions were positive for p16 while only some of the low-grade lesions were positive. The results obtained in histological samples could be extrapolated to cytological samples from the same patients. Conclusions: p16 expression in conventional cytology provides similar results to those in histological samples (AU)
Subject(s)
Female , Humans , Pregnancy , Uterine Cervical Neoplasms/drug therapy , Uterine Cervical Neoplasms/radiotherapy , Cell Biology/education , Cyclin-Dependent Kinase Inhibitor p16/administration & dosage , Cyclin-Dependent Kinase Inhibitor p16 , Papilloma/genetics , Papanicolaou Test/methods , Uterine Cervical Neoplasms/rehabilitation , Uterine Cervical Neoplasms/therapy , Cell Biology/instrumentation , Cyclin-Dependent Kinase Inhibitor p16/metabolism , Cyclin-Dependent Kinase Inhibitor p16/supply & distribution , Papilloma/metabolism , Papanicolaou TestABSTRACT
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Subject(s)
Female , Humans , Papilloma/pathology , Papilloma/virology , Papillomavirus Vaccines/administration & dosage , Primary Prevention/methods , Secondary Prevention/methods , Uterine Cervical Neoplasms/drug therapy , Vulvar Neoplasms/metabolism , Papilloma/genetics , Papilloma/metabolism , Uterine Cervical Neoplasms/radiotherapy , Papillomavirus Vaccines , Primary Prevention/instrumentation , Secondary Prevention , Uterine Cervical Neoplasms/therapy , Vulvar Neoplasms/pathologyABSTRACT
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Subject(s)
Female , Humans , Papilloma/pathology , Papilloma/virology , Primary Prevention/methods , Secondary Prevention/methods , Uterine Cervical Neoplasms/drug therapy , Vulvar Neoplasms/therapy , Papilloma/genetics , Papilloma/metabolism , Primary Prevention , Uterine Cervical Neoplasms/therapy , Vulvar Neoplasms/drug therapyABSTRACT
FUNDAMENTOS: Acrocórdons são lesões dermatológicas comuns na população e estão associados ao diabetes mellitus, à obesidade, à resistência insulínica e à aterosclerose. A identificação precoce de pacientes com resistência insulínica pode ter papel preventivo primário. OBJETIVO: Avaliar a associação entre presença de acrocórdons cervicais ou axilares e resistência insulínica. MÉTODOS: Estudo transversal com pacientes dermatológicos adultos atendidos em hospital universitário. Casos foram definidos como portadores de mais de cinco acrocórdons cervicais e/ou axilares. A resistência insulínica foi estimada pelo índice HOMA-IR. Resultados foram ajustados pelas demais covariáveis de risco para resistência insulínica conhecidos, a partir de regressão logística múltipla. RESULTADOS: Avaliaram-se 98 casos e 103 controles, que não diferiram entre si quanto à idade ou ao gênero. Acrocórdons se associaram diretamente aos valores de HOMA-IR (Odds Ratio = 1,4), hipertrigliceridemia e índice de massa corpórea, independentemente do ajuste por diabetes mellitus, idade, fototipo, gênero, história de diabetes mellitus familiar e relação cintura/quadril. Níveis qualitativamente elevados de HOMA-IR (> 3,8) também evidenciaram associação significativa (Índice de probabilidade = 7,5). CONCLUSÕES: Presença de múltiplos acrocórdons se associou à resistência insulínica, independentemente dos demais fatores de risco.
BACKGROUND: Skin tags are dermatological lesions commonly found in the general population and have been associated with diabetes mellitus, obesity, insulin resistance and atherosclerosis. Early detection of patients with insulin resistance may play an important preventive role. OBJECTIVE: To evaluate the association between skin tags in the neck or axillary regions and insulin resistance. METHODS: A cross-sectional study involving adult patients receiving care at a university teaching hospital. Cases were defined as patients with > 5 skin tags in the neck region and/or axillae. Insulin resistance was estimated using the HOMA-IR index. Results were adjusted for the other known covariates of risk for insulin resistance using a multiple logistic regression model. RESULTS: Ninety-eight cases and 103 controls were evaluated. There was no difference between the groups with respect to age or gender. Skin tags were directly associated with HOMA-IR values (odds ratio = 1.4), hypertriglyceridemia and body mass index, irrespective of adjustment for diabetes mellitus, age, skin phototype, gender, family history of diabetes mellitus or hip/waist ratio. Qualitatively elevated HOMA-IR levels (>3.8) were also significantly associated (odds ratio = 7.5). CONCLUSIONS: The presence of multiple skin tags was strongly associated with insulin resistance irrespective of other risk factors.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Insulin Resistance , Papilloma/metabolism , Skin Neoplasms/metabolism , Case-Control Studies , Cross-Sectional Studies , Risk FactorsABSTRACT
INTRODUÇÃO E OBJETIVO: O tipo de câncer oral mais frequente é o carcinoma de células escamosas, que corresponde a 95 por cento dos casos(9). O papiloma escamoso oral é uma neoplasia benigna normalmente associada à infecção pelo papilomavírus humano (HPV)(21). A análise da literatura mostra alterações nos genes reguladores do ciclo celular p27, p21WAF/Cip1 e p16INK4a, porém sem uma definição de seus papéis na carcinogênese oral. O objetivo foi caracterizar imuno-histoquimicamente p27, p21WAF/Cip1 e p16NK4a em epitélio escamoso normal, papilomas escamosos e carcinomas de células escamosas da cavidade oral. MÉTODOS: Imuno-histoquímica para p27, p21WAF/Cip1 e p16NK4a em 32 casos de epitélio escamoso normal, 30 casos de papiloma escamoso e 34 de carcinoma de células escamosas da cavidade oral. RESULTADOS: p27: 97,06 por cento dos casos de carcinoma de células escamosas apresentaram imunopositividade focal. O grupo papiloma escamoso apresentou 33,33 por cento e o grupo controle, 18,75 por cento. p21WAF/Cip1: 100 por cento de imunopositividade focal tanto no grupo controle como no grupo carcinoma de células escamosas, e 90 por cento no grupo papiloma escamoso. p16INK4a: 100 por cento de imunopositividade focal para os grupos controle e papiloma escamoso, e 94 por cento para o grupo carcinoma de células escamosas. CONCLUSÃO: Imuno-histoquimicamente demonstrou-se diferença significativa para p27 quando feita comparação dos grupos controle e papiloma escamoso com o grupo carcinoma de células escamosas. O p21WAF/Cip1 não demonstrou poder de diferenciar os grupos analisados. O p16INK4a apresentou imunopositividade difusa em uma minoria dos casos do grupo carcinoma de células escamosas. O grupo papiloma escamoso se comportou de maneira similar ao grupo controle em relação aos três marcadores.
INTRODUCTION: The most frequent type of oral cancer is the squamous cell carcinoma, which corresponds to 95 percent of the cases(9).The oral squamous papilloma is a benign neoplasia, commonly associated with infections caused by the human papilloma virus(21). The analysis of medical literature shows changes in cell cycle regulatory genes (p27, p21WAF/Cip1 and p16INK4a), but does not define their roles in oral carcinogenesis. Objective: Characterize the immuno-histochemical expression of p27, p21WAF/Cip1 and p16INK4a in oral normal squamous epithelium, oral squamous papilloma and oral squamous cell carcinoma. METHODS: Immuno-histochemical evaluation of p27, p21WAF/Cip1 and p16INK4a in 32 samples of oral normal squamous epithelium, 30 of oral squamous papilloma and 34 of oral squamous cell carcinoma. RESULTS: 97.06 percent of the oral squamous cell carcinoma group, 33.33 percent of the squamous papilloma group and 18.75 percent of the control group showed focal immunopositivity for p27. 100 percent of both control and oral squamous cell carcinoma groups and 90 percent of the oral squamous papilloma group showed focal immunopositivity for p21WAF/Cip1. 100 percent of both control and oral squamous papilloma groups and 94 percent of the oral squamous cell carcinoma group showed focal immunopositivity for p16INK4a. CONCLUSIONS: The study revealed a statistically significant difference for p27 expression when comparing the control and oral squamous papilloma groups with the oral squamous cell carcinoma group. p21WAF/Cip1 did not prove to be useful to differentiate the groups. p16INK4a showed diffuse immunopositivity in a minority of the oral squamous cell carcinoma cases. The oral squamous papilloma group behaved similarly to the control group as to the three markers.
Subject(s)
Humans , Male , Female , Adult , Carcinoma, Squamous Cell/metabolism , Mouth Neoplasms/metabolism , Papilloma/metabolism , Immunohistochemistry , Retrospective StudiesABSTRACT
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Subject(s)
Humans , Male , Female , Papilloma/metabolism , Papilloma/pathology , Uterine Cervical Neoplasms/drug therapy , Polymerase Chain Reaction/methods , Menopause/metabolism , Sexually Transmitted Diseases, Viral/transmission , Sexually Transmitted Diseases, Viral/virology , Uterine Cervical Neoplasms/psychology , Papilloma/complications , Papilloma/diagnosis , Uterine Cervical Neoplasms/complications , Polymerase Chain Reaction/classification , Menopause/genetics , Sexually Transmitted Diseases, Viral/metabolism , Sexually Transmitted Diseases, Viral/pathology , Prospective Studies , Uterine Cervical Neoplasms/metabolismABSTRACT
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Subject(s)
Humans , Male , Female , Papilloma/metabolism , Papilloma/virology , Reproductive Tract Infections/transmission , Reproductive Tract Infections/virology , Vulvar Neoplasms/pathology , Muscular Dystrophies/pathology , Therapeutics/methods , Papilloma/complications , Papilloma/diagnosis , Reproductive Tract Infections/metabolism , Reproductive Tract Infections/pathology , Vulvar Neoplasms/virology , Muscular Dystrophies/metabolism , Therapeutics/instrumentationABSTRACT
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Subject(s)
Humans , Male , Female , Papilloma/diagnosis , Papilloma/virology , Uterine Cervical Neoplasms/pathology , Reproductive Tract Infections/virology , Viremia/virology , Lymph Nodes/pathology , Hepatitis B/virology , Public Health/methods , Papilloma/metabolism , Papilloma/pathology , Uterine Cervical Neoplasms/virology , Reproductive Tract Infections/transmission , Viremia/transmission , Lymph Nodes/metabolism , Hepatitis B/transmission , Public Health/legislation & jurisprudenceABSTRACT
Por ser um exame fundamental para o estabelecimento do plano terapêutico em pacientes com descarga papilar espontânea, a ductografia, tal como é realizada no INCa, é descrita em seus procedimentos e análises. A partir da reuniäo de treze casos registrados naquela instituiçäo, os elementos definidores do correto diagnóstico, assim como os procedimentos complementares utilizados, säo demonstrados de modo simples e objetivo