ABSTRACT
The 16th non-collagenous domain (NC16A) of BP180 is the main antigenic target of autoantibodies in bullous pemphigoid (BP) and mucous membrane pemphigoid (MMP). Commercially available assays detect serum autoantibodies against NC16A in the majority of BP (80%-90%) and in approximately 50% of MMP patients. However, a standardized test system for detecting antibodies against other regions of BP180 is still lacking. Moreover, anti-BP180 autoantibodies have been found in neurological conditions such as multiple sclerosis and Parkinson disease. This study aimed at identifying primary epitopes recognized by BP autoantibodies on the BP180 ectodomain. Serum samples of 51 BP and 30 MMP patients both without anti-NC16A reactivity were included along with 44 multiple sclerosis and 75 Parkinson disease sera. Four overlapping His-tagged proteins covering the entire BP180 ectodomain (BP180(ec)1-4) were cloned, expressed, purified and tested for reactivity by immunoblot. IgG antibodies to BP180(ec)3 were detected in 98% of BP, 77% of MMP and 2% of normal human sera. Only weak reactivity was detected for neurological diseases against BP180(ec)1, BP180(ec)2 and BP180(ec)4, in 3%, 11% and 7% of tested multiple sclerosis sera, respectively. 8% of Parkinson disease sera reacted with BP180(ec)2 and 9% with BP180(ec)4. In conclusion, this study successfully identified epitopes recognized by BP autoantibodies outside the NC16A domain in pemphigoid diseases. These findings contribute to a better understanding of the immune response in BP and MMP with potential implications for a future diagnostic assay for NC16A-negative pemphigoid patients.
Subject(s)
Autoantibodies , Autoantigens , Collagen Type XVII , Multiple Sclerosis , Non-Fibrillar Collagens , Parkinson Disease , Pemphigoid, Benign Mucous Membrane , Pemphigoid, Bullous , Humans , Parkinson Disease/immunology , Parkinson Disease/blood , Non-Fibrillar Collagens/immunology , Pemphigoid, Bullous/immunology , Pemphigoid, Bullous/blood , Autoantigens/immunology , Multiple Sclerosis/immunology , Multiple Sclerosis/blood , Autoantibodies/blood , Autoantibodies/immunology , Pemphigoid, Benign Mucous Membrane/immunology , Pemphigoid, Benign Mucous Membrane/blood , Immunoglobulin G/blood , Immunoglobulin G/immunology , Epitopes/immunology , Protein Domains , Female , Male , AgedABSTRACT
BACKGROUND: Bullous pemphigoid (BP), the most common subepidermal autoimmune blistering disease, is classically defined by the presence of IgG autoantibodies directed against the hemidesmosomal proteins BP180 (type XVII collagen) and BP230 and the predominance of skin lesions. Several studies have addressed the role of anti-BP180 IgE in patients and experimental models, while data on anti-BP230 IgE are scarce. OBJECTIVE: To assess anti-BP230 IgE level by ELISA in BP sera and to correlate it with disease severity and clinical characteristics. METHODS: BP sera underwent anti-BP230 IgE ELISA and Western blotting against human BP230 fragments. RESULTS: We demonstrate that 36/154 (23%) of BP sera were positive for anti-BP230 IgE. Anti-BP230 IgE levels had no correlation with clinical phenotype or disease activity per se. Interestingly, anti-BP230 IgE was significantly associated with disease activity within individuals during the course of the disease. Additionally, anti-BP230 IgE and total IgE levels showed a significant correlation. Notably, anti-BP230 IgG correlated interindividually with disease activity. By Western blotting, the C-terminal domain of BP230 fragments (C2; amino acids 2024-2349 and C3; amino acids 2326-2649), provided the best serological assay for anti-BP230 IgE detection. CONCLUSION: As a complementary tool, IgE immunoblotting is recommended to obtain an optimal serological diagnosis, particularly in patients with severe disease without IgG reactivity by BP180- or BP230-specific ELISA. Although the detection of serum anti-BP230 IgE is not of major diagnostic significance, it may be relevant for therapeutic decisions, e.g., for anti-IgE-directed treatment, which has been successfully used in case series of BP.
Subject(s)
Autoantibodies , Autoantigens , Dystonin , Enzyme-Linked Immunosorbent Assay , Immunoglobulin E , Immunoglobulin G , Non-Fibrillar Collagens , Pemphigoid, Bullous , Severity of Illness Index , Humans , Pemphigoid, Bullous/immunology , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/diagnosis , Immunoglobulin E/blood , Immunoglobulin E/immunology , Autoantibodies/blood , Autoantibodies/immunology , Male , Female , Aged , Autoantigens/immunology , Dystonin/immunology , Aged, 80 and over , Non-Fibrillar Collagens/immunology , Middle Aged , Immunoglobulin G/blood , Immunoglobulin G/immunology , Collagen Type XVII , Adult , Blotting, WesternSubject(s)
Laminin , Pemphigoid, Bullous , Humans , Pemphigoid, Bullous/diagnosis , Pemphigoid, Bullous/immunology , Pemphigoid, Bullous/blood , Laminin/immunology , Sensitivity and Specificity , Recombinant Proteins/immunology , Autoantibodies/blood , Autoantibodies/immunology , Enzyme-Linked Immunosorbent Assay , Female , Male , Aged , Serologic Tests/methodsABSTRACT
Bullous pemphigoid (BP), the most frequent blistering dermatosis in the elderly, is associated with increased mortality. The severity of BP can be assessed by detecting the anti-BP180 immunoglobulin G (IgG) concentration, but the lab test is not available in many community clinics. BP patients are usually in a hypercoagulable state with increased levels of D-dimer and fibrin degradation products (FDPs). We aimed to evaluate the use of D-dimer and FDPs in assessing BP severity. We compared the levels of plasma D-dimer, plasma FDPs, eosinophil counts, eosinophil cationic protein, and serum anti-BP180 IgG concentration between 48 typical BP patients and 33 Herpes zoster (HZ) patients (control group). Correlational analyses were conducted to determine the relationships between the lab values and common BP severity markers. The plasma D-dimer and FDP levels were higher in BP patients than in HZ controls (D-dimer: 3297 ± 2517 µg/L vs. 569.70 ± 412.40 µg/L; FDP: 9.74 ± 5.88 mg/L vs. 2.02 ± 1.69 mg/L, respectively, P < 0.0001). Significant positive correlations were found between D-dimer/FDP levels and BP severity markers (i.e. anti-BP180 IgG concentration [D-dimer: r = 0.3928, P = 0.0058; FDP: r = 0.4379, P = 0.0019] and eosinophil counts [D-dimer: r = 0.3625, P = 0.0013; FDP: r = 0.2880, P = 0.0472]) in BP patients. We also found an association between FDP and urticaria/erythema lesions (r = 0.3016, P = 0.0372), but no other BPDAI components. In 19 BP patients with complete remission after systemic glucocorticoid treatment, D-dimer and FDP levels decreased post-therapy (D-dimer: 5559 ± 7492 µg/L vs. 1738 ± 1478 µg/L; P < 0.0001; FDP: 11.20 ± 5.88 mg/L vs. 5.13 ± 3.44 mg/L; P = 0.0003), whereas they did not in BP patients with treatment resistant. Plasma D-dimer and FDP are convenient markers to evaluate BP severity assistant on BPDAI and eosinophil counts. FDP is also helpful for inflammatory lesions in BP patients.
Subject(s)
Fibrin Fibrinogen Degradation Products/analysis , Pemphigoid, Bullous/blood , Aged , Aged, 80 and over , Autoantibodies/blood , Autoantigens/immunology , Biomarkers , Cross-Sectional Studies , Eosinophil Cationic Protein/blood , Eosinophilia/blood , Eosinophilia/etiology , Female , Herpes Zoster/blood , Humans , Immunoglobulin G/blood , Immunoglobulin G/immunology , Male , Middle Aged , Non-Fibrillar Collagens/immunology , Pemphigoid, Bullous/complications , Severity of Illness Index , Thrombophilia/blood , Thrombophilia/etiology , Urticaria/blood , Collagen Type XVIIABSTRACT
BACKGROUND: The clinical and immunological profile of patients with dipeptidyl peptidase-4 inhibitor (DPP4i)-associated bullous pemphigoid (BP) is inconsistent in the current literature. OBJECTIVES: The aims were to investigate the clinical and immunological features of patients with DPP4i-associated BP and to examine whether there are intraclass differences between different DPP4i agents. METHODS: A retrospective cohort study was conducted, including all consecutive patients diagnosed with BP throughout the years 2009-2019 in a tertiary referral center. RESULTS: The study encompassed 273 patients with BP (mean age at diagnosis 79.1 ± 9.9 years), of whom 24 (8.8%) were associated with DPP4i. Sitagliptin was the prescribed agent for 17 patients (70.8%), and vildagliptin was prescribed in seven patients (29.2%). Relative to other patients with BP, patients with DPP4i-associated BP had more prominent truncal involvement (95.8% vs. 73.9%; P = 0.017), greater erosion/blister Bullous Pemphigoid Disease Area Index (BPDAI) subscore (29.8 ± 17.4 vs. 20.6 ± 14.4; P = 0.018), and lower levels of anti-BP180 NC16A (279.2 ± 346.1 vs. 572.2 ± 1352.0 U/ml; P = 0.009) and anti-BP230 (25.5 ± 47.8 vs. 128.6 ± 302.9 U/ml; P = 0.009) antibodies. Relative to patients with sitagliptin-associated BP, those with vildagliptin-associated BP had a lower seropositivity rate (57.1% vs. 94.1%, P = 0.031) and lower levels (96.7 ± 139.0 vs. 354.5 ± 376.5; P = 0.023) of anti-BP180 NC16A antibodies, and tended to present with higher erosion/blister BPDAI subscore (36.3 ± 9.6 vs. 25.8 ± 19.7; P = 0.095). CONCLUSIONS: DPP4i-associated BP is characterized by a more severe blistering and erosive presentation despite lower levels of typically pathogenic antibodies.
Subject(s)
Autoantibodies/blood , Dipeptidyl-Peptidase IV Inhibitors/adverse effects , Pemphigoid, Bullous/diagnosis , Skin/pathology , Aged , Aged, 80 and over , Autoantibodies/immunology , Autoantigens/immunology , Dystonin/immunology , Female , Humans , Male , Middle Aged , Non-Fibrillar Collagens/immunology , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/chemically induced , Pemphigoid, Bullous/immunology , Retrospective Studies , Severity of Illness Index , Sitagliptin Phosphate/adverse effects , Skin/immunology , Vildagliptin/adverse effects , Collagen Type XVIIABSTRACT
BACKGROUND: TH17/IL-23 immune axis is considered to be involved in the pathogenesis of autoimmune and chronic inflammatory diseases. Bullous pemphigoid (BP) is the most frequent autoimmune blistering disease, characterized by the presence of autoantibodies against the components of the dermal-epidermal junction. Animal studies and characterization of patient samples point toward a contribution of TH17 cells in BP pathogenesis. However, genetic polymorphisms in the genes of TH17/IL-23 cytokines have not yet been well investigated in BP. METHODS: Detection of polymorphisms in IL-17A (rs2275913 and rs3819025), IL-17F (rs2397084 and rs763780), IL-17RA (rs2229151), and IL-23R (rs2201841, rs7530511, rs11209026, and rs10889677) genes were performed following the collection of blood samples and DNA extraction from BP patients and controls. Gene expression of IL-23R was determined by quantitative RT-PCR analysis. RESULTS: The prevalence of IL-23R rs7530511 genotypes and alleles, as well as IL-23R rs2201841 alleles, is significantly different between the BP patients and controls. While the minor C-allele of IL-23R rs7530511 is highly present in the patients, the G-allele distribution of IL-23R rs2201841 is significantly more prevalent in the control individuals compared to the BP patients. Genotypes and alleles of other SNPs in IL-17A, IL-17F, and IL-17RA were similarly distributed in patients and controls. CONCLUSIONS: No alteration was found in the gene expression between wild and polymorphic genotypes of IL-23R (rs2201841 and rs7530511) variations, indicating they do not contribute to altering the levels of gene expression in blood. In summary, our data show that the alleles of two SNPs in IL-23R rs2201841 and rs7530511 are associated with BP.
Subject(s)
Interleukin-17/genetics , Pemphigoid, Bullous/genetics , Polymorphism, Single Nucleotide , Receptors, Interleukin-17/genetics , Receptors, Interleukin/genetics , Aged , Female , Humans , Interleukin-17/blood , Male , Pemphigoid, Bullous/blood , Receptors, Interleukin/blood , Receptors, Interleukin-17/blood , Th17 Cells/metabolismABSTRACT
Bullous pemphigoid (BP) is an autoimmune blistering disease characterized by recruitment of leucocytes into skin and release of damaging enzymes, resulting in epidermal detachment and blister formation. To better understand the role of leukotriene B4 (LTB4) and other inflammatory factors in BP pathophysiology, we conducted microscopic and immunohistochemical analyses of preserved skin biopsy sections and conducted flow cytometry and ELISA analyses of matched blood and blister fluid from BP patients. Neutrophils predominated in BP blister fluid, which also contained monocytes/macrophages and T cells, but few to no eosinophils and B cells. In contrast, BP skin histology showed a different pattern, with abundant neutrophils but eosinophils being the predominant immune cell type. LTB4 pathway and neutrophil activation markers were prevalent in BP skin lesions and strongly associated with perivascular neutrophils. Blister fluid neutrophils, monocytes/macrophages and eosinophils all exhibited increased surface expression of leukotriene A4 hydrolase and neutrophil elastase (P = .002 for both). Blister fluid was also enriched in interleukins (IL)-1α, IL-1ß, IL-8, IL-10, IL-18, monocyte colony-stimulating factor (M-CSF) and vascular endothelial growth factor (VEGF). Our findings suggest differential leucocyte recruitment from blood into dermis and from dermis into blister, which correlates with disease activity, and presents potential new treatment opportunities for BP.
Subject(s)
Exudates and Transudates/cytology , Leukotriene B4/metabolism , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/pathology , Skin/pathology , Aged , Aged, 80 and over , Eosinophils , Epoxide Hydrolases/metabolism , Exudates and Transudates/metabolism , Female , Flow Cytometry , Humans , Interleukins/metabolism , Leukocyte Elastase/metabolism , Macrophage Colony-Stimulating Factor/metabolism , Macrophages/enzymology , Male , Middle Aged , Monocytes/enzymology , Neutrophil Infiltration , Neutrophils/enzymology , Pemphigoid, Bullous/immunology , Race Factors , Sex Factors , Skin/metabolism , Vascular Endothelial Growth Factor A/metabolismABSTRACT
Dermatitis herpetiformis (DH) is an extraintestinal manifestation of coeliac disease (CD). Patients with DH have an elevated risk of development of another autoimmune blistering skin disease, bullous pemphigoid (BP). In this study we investigated whether patients with DH and CD (mean age for both 49 years) have circulating autoantibodies against BP180, the major BP autoantigen. ELISA tests showed that only a few DH (3/46) and CD (2/43) patients had BP180-NC16A IgG autoantibodies. Immunoblotting found that more than half of the DH samples contained IgG autoantibodies against full-length BP180. Epitope mapping with 13 fusion proteins covering the BP180 polypeptide revealed that in DH and CD patients, IgG autoantibodies did not target the NC16A or other epitopes typical of BP but recognized other intracellular and mid-extracellular regions of BP180. None of the analyzed DH and CD patients with either ELISA or immunoblotting positivity had IgG or IgA reactivity against the cutaneous basement membrane in indirect immunofluorescence analysis or skin symptoms characteristic of BP. Although only a minority of middle-aged DH patients had IgG autoantibodies against the immunodominant epitopes of BP180, our results do not exclude the possibility that intermolecular epitope spreading could explain the switch from DH to BP in elderly patients.
Subject(s)
Autoantibodies/blood , Autoantigens/immunology , Autoimmunity , Celiac Disease/immunology , Dermatitis Herpetiformis/immunology , Immunodominant Epitopes , Immunoglobulin G/blood , Non-Fibrillar Collagens/immunology , Pemphigoid, Bullous/immunology , Adult , Aged , Aged, 80 and over , Case-Control Studies , Celiac Disease/blood , Celiac Disease/diagnosis , Dermatitis Herpetiformis/blood , Dermatitis Herpetiformis/diagnosis , Enzyme-Linked Immunosorbent Assay , Epitope Mapping , Female , GTP-Binding Proteins/immunology , Humans , Male , Middle Aged , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/diagnosis , Protein Glutamine gamma Glutamyltransferase 2 , Transglutaminases/immunology , Collagen Type XVIIABSTRACT
BACKGROUND: Regulatory T (Treg) cells play an essential role in peripheral immune tolerance. Bullous pemphigoid (BP) is the most common blistering disease and is caused by autoantibodies to two BP antigens: type XVII collagen and BP230. Recently, we reported that Treg cell dysfunction may cause the production of autoantibodies to BP antigens. Several studies have suggested an association between Treg cells and BP pathogenesis. However, Treg cells are heterogeneous in humans, leading to inconsistent results in previous studies. OBJECTIVE: To assess functional Treg subsets in BP. METHODS: We examined three distinct Treg subsets in conventional BP (cBP) patients before versus after systemic corticosteroid treatment, dipeptidyl peptidase-4 inhibitor-associated BP (DPP-4i-BP) patients, younger controls and older controls. RESULTS: We found that total Treg cells and all Treg cell subsets were increased in cBP patients before treatment and decreased by systemic corticosteroid treatment. In contrast, neither total Treg cells nor all Treg subsets were increased in DPP-4i-BP. Notably, CD45RA- Foxp3hi effector Treg cells positively correlated with disease severity in cBP, whereas CD45RA+Foxp3lo naïve Treg cells positively correlated with the disease severity in DPP-4i-BP. CONCLUSION: These findings suggest that Treg cells are differently involved in the pathogeneses of cBP and DPP-4i-BP.
Subject(s)
Autoantibodies/immunology , Dipeptidyl-Peptidase IV Inhibitors/adverse effects , Pemphigoid, Bullous/immunology , T-Lymphocyte Subsets/immunology , T-Lymphocytes, Regulatory/immunology , Adult , Aged , Aged, 80 and over , Autoantibodies/metabolism , Autoantigens/immunology , CD4 Lymphocyte Count , Case-Control Studies , Diabetes Mellitus, Type 2/blood , Diabetes Mellitus, Type 2/drug therapy , Dystonin/immunology , Female , Glucocorticoids/administration & dosage , Healthy Volunteers , Humans , Male , Middle Aged , Non-Fibrillar Collagens/immunology , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/chemically induced , Pemphigoid, Bullous/diagnosis , Severity of Illness Index , T-Lymphocyte Subsets/drug effects , T-Lymphocyte Subsets/metabolism , T-Lymphocytes, Regulatory/drug effects , T-Lymphocytes, Regulatory/metabolism , Collagen Type XVIIABSTRACT
Although T cells are considered as the central component in immune-mediated diseases, supportive evidence has demonstrated that B cells also contribute to the progression of these diseases. B cells are divided into various subsets according to their secreted cytokines. Different B cell subsets play diverse roles in immune-mediated dermatoses. Regulatory B cells (Bregs) are defined functionally by their ability to secrete IL-10, which has been revealed to contribute to immunological tolerance. Drugs that deplete B cells, such as rituximab, are now used for the treatment of several immune-mediated dermatoses. In this review, we present and discuss the current knowledge on the roles of B cells in several immune-mediate dermatoses including psoriasis, pemphigus, bullous pemphigoid, and dermatomyositis, atopic dermatitis.
Subject(s)
B-Lymphocytes, Regulatory/immunology , Dermatitis, Atopic/immunology , Dermatomyositis/immunology , Pemphigoid, Bullous/immunology , Pemphigus/immunology , Psoriasis/immunology , Animals , B-Lymphocytes, Regulatory/drug effects , B-Lymphocytes, Regulatory/metabolism , Dermatitis, Atopic/blood , Dermatitis, Atopic/drug therapy , Dermatitis, Atopic/pathology , Dermatomyositis/blood , Dermatomyositis/drug therapy , Dermatomyositis/pathology , Desmoglein 3/metabolism , Disease Models, Animal , Humans , Immunologic Factors/pharmacology , Immunologic Factors/therapeutic use , Interleukin-10/metabolism , Lymphocyte Depletion/methods , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/drug therapy , Pemphigoid, Bullous/pathology , Pemphigus/blood , Pemphigus/drug therapy , Pemphigus/pathology , Psoriasis/blood , Psoriasis/drug therapy , Psoriasis/pathology , Skin/immunology , Skin/pathologyABSTRACT
BACKGROUND: Paraneoplastic pemphigus (PNP) is a devastating autoimmune multiorgan syndrome associated with autoantibodies against several autoantigens, including the alpha-2-macroglobulin-like-1 (A2ML1). A2ML1 is recognized by up to 70 % of PNP sera. The currently recommended techniques for serological diagnosis of PNP are inadequate to detect anti-A2ML1 antibodies. OBJECTIVES: To develop novel assays which allow to easily and reliably detect anti-A2ML1 autoantibodies in PNP sera. METHODS: We produced full-length A2ML1 in fusion with enhanced green fluorescent protein (EGFP-A2ML1) in transfected human embryonic kidney 293 T cells. The recombinant protein was used as fluorescent ligand for immunoprecipitation studies. We further developed an enzyme-linked immunosorbent assay (ELISA) by immobilizing EGFP-A2ML1 on 96-well plates. RESULTS: A2ML1-positive PNP sera were able to immunoprecipitate EGFP-A2ML1. Direct measurement of fluorescence in immunoprecipitates correlates with the relative levels of anti-A2ML1 antibodies in the PNP sera. By the novel ELISA, based on the determined best cut-off value, 61 % of the tested 36 PNP sera were A2ML1 positive with a specificity of 88.9 % and a sensitivity of 95 %. The 20 tested normal sera (NHS) were negative, while 2 (10 %) of 20 pemphigus vulgaris and 3 (15 %) of 20 bullous pemphigoid sera showed borderline values. CONCLUSIONS: Our novel immunoassays enable rapid stratification of PNP patients. The novel green fluorescent protein-based ELISA utilizing an active eukaryotic A2ML1 is highly sensitive and reliable and, hence, is useful for a better understanding of the immunological background of PNP. This approach may be easily applied for the rapid detection of antibodies to various other antigens.
Subject(s)
Autoantibodies/isolation & purification , Paraneoplastic Syndromes/diagnosis , Pemphigoid, Bullous/diagnosis , Pemphigus/diagnosis , alpha-Macroglobulins/immunology , Autoantibodies/blood , Autoantibodies/immunology , Diagnosis, Differential , Enzyme-Linked Immunosorbent Assay/methods , Green Fluorescent Proteins/genetics , HEK293 Cells , Humans , Paraneoplastic Syndromes/blood , Paraneoplastic Syndromes/immunology , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/immunology , Pemphigus/blood , Pemphigus/immunology , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/immunology , Sensitivity and Specificity , alpha-Macroglobulins/geneticsABSTRACT
BACKGROUND: Bullous pemphigoid (BP) is a common T helper 2- (Th2-) dominated autoimmune blistering skin disease with significant mortality. MicroRNAs (miRNAs), which are endogenous noncoding RNA molecules, have been reported to be potential biomarkers for some autoimmune diseases; however, to date, there exist no reports on serum expression profiles of miRNAs in BP patients. METHODS: A RNA quantitative PCR- (qPCR-) based array was conducted on sera from 20 active BP patients and 20 healthy controls for screening of miRNAs. Significantly dysregulated miRNAs were validated with use of qPCR as performed on sera samples of 45 active BP patients and 60 healthy controls. Serum CCL17, anti-BP180, and anti-BP230 levels were measured with use of ELISA. RESULTS: Relative baseline expression levels of serum miR-1291 were significantly upregulated in the 45 BP patients as compared with the 60 healthy controls (P < 0.001) and significantly decreased in the disease control stage (n = 13, P = 0.006). In addition, these baseline miR-1291 levels showed a significant positive correlation with the baseline levels of serum CCL17 (P < 0.001) and anti-BP180 (n = 38, P = 0.024). Like that observed for miR-1291, baseline levels of serum CCL17 were also significantly elevated in the 45 BP patients compared with the 60 healthy controls (P < 0.001) and significantly decreased in the disease control stage (n = 13, P = 0.002). However, for anti-BP180, baseline serum levels were significantly elevated in only 38 of the 45 BP patients and significantly decreased in the disease control stage (n = 10, P = 0.004). CONCLUSIONS: Relative expression levels of serum miR-1291 can reflect disease activity of BP. miR-1291 may function as an important new serum biomarker for BP.
Subject(s)
Chemokine CCL17/genetics , MicroRNAs/genetics , Pemphigoid, Bullous/diagnosis , Pemphigoid, Bullous/genetics , Adult , Aged , Aged, 80 and over , Autoantibodies/blood , Autoantibodies/genetics , Autoantigens/blood , Autoantigens/genetics , Autoantigens/immunology , Biomarkers/blood , Case-Control Studies , Chemokine CCL17/blood , Chemokine CCL17/immunology , Dystonin/blood , Dystonin/genetics , Dystonin/immunology , Female , Humans , Male , MicroRNAs/blood , MicroRNAs/immunology , Middle Aged , Non-Fibrillar Collagens/blood , Non-Fibrillar Collagens/genetics , Non-Fibrillar Collagens/immunology , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/immunology , Skin/immunology , Skin/pathology , Th2 Cells/immunology , Th2 Cells/pathology , Collagen Type XVIIABSTRACT
No Abstract.
Subject(s)
Autoantigens/blood , Dystonin/blood , Interleukin-10/blood , Interleukin-18/blood , Non-Fibrillar Collagens/blood , Pemphigoid, Bullous/blood , Aged , Anti-Inflammatory Agents/therapeutic use , Female , Humans , Iran , Male , Middle Aged , Pemphigoid, Bullous/drug therapy , Pemphigoid, Bullous/immunology , Pemphigoid, Bullous/therapy , Collagen Type XVIIABSTRACT
BACKGROUND: The current standard in the serologic diagnosis of autoimmune bullous diseases (AIBD) is a multistep procedure sequentially applying different assays. In contrast, the BIOCHIP Mosaic technology combines multiple substrates for parallel analysis by indirect immunofluorescence. METHODS: Sera from 749 consecutive, prospectively recruited patients with direct immunofluorescence-positive AIBD from 13 international study centers were analyzed independently and blinded by using (1) a BIOCHIP Mosaic including primate esophagus, salt-split skin, rat bladder, monkey liver, monkey liver with serosa, recombinant BP180 NC16A, and gliadin GAF3X, as well as HEK293 cells expressing recombinant desmoglein 1, desmoglein 3, type VII collagen, and BP230 C-terminus and (2) the conventional multistep approach of the Department of Dermatology, University of Lübeck. RESULTS: In 731 of 749 sera (97.6%), specific autoantibodies could be detected with the BIOCHIP Mosaic, similar to the conventional procedure (725 cases, 96.8%). The Cohen κ for both serologic approaches ranged from 0.84 to 1.00. In 6.5% of sera, differences between the 2 approaches occurred and were mainly attributed to autoantigen fragments not present on the BIOCHIP Mosaic. LIMITATIONS: Laminin 332 and laminin γ1 are not represented on the BIOCHIP Mosaic. CONCLUSIONS: The BIOCHIP Mosaic is a standardized time- and serum-saving approach that further facilitates the serologic diagnosis of AIBD.
Subject(s)
Autoimmune Diseases/diagnosis , Pemphigoid, Bullous/diagnosis , Pemphigoid, Bullous/immunology , Pemphigus/diagnosis , Pemphigus/immunology , Adolescent , Adult , Aged , Aged, 80 and over , Autoimmune Diseases/blood , Child , Female , Fluorescent Antibody Technique, Indirect/methods , Humans , Male , Middle Aged , Pemphigoid, Bullous/blood , Pemphigus/blood , Prospective Studies , Young AdultSubject(s)
Paraplegia/complications , Pemphigoid, Bullous/pathology , Spinal Cord Injuries/complications , Biopsy , Dermatologic Agents/administration & dosage , Dermatologic Agents/therapeutic use , Female , Humans , Methotrexate/administration & dosage , Methotrexate/therapeutic use , Middle Aged , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/diagnosis , Pemphigoid, Bullous/drug therapy , Treatment OutcomeABSTRACT
iNKT (invariant natural killer T) cells are unconventional immunoregulatory T cells which contribute to B cell maturation, antibody and cytokine production. iNKT cells are implicated in the control of autoimmune inflammation in different disorders. For bullous pemphigoid (BP), the most frequent bullous autoimmune dermatosis, the role of iNKT cells has not yet been studied. We, therefore, aimed at investigating the frequency of iNKT cells in peripheral blood and biopsies from lesional and non-lesional skin from patients with BP and controls. Circulating CD3+iTCR+ iNKT cells were assessed by flow cytometry in peripheral blood from 30 patients with BP and from 29 controls (19 patients with skin tumors and 10 healthy controls). In 34 lesional and 13 non-lesional skin biopsies from BP patients and 17 biopsies from control individuals the number of Vα24+Vß11+ iNKT cells was investigated by immunofluorescence staining. BP patients showed a significantly lower frequency of circulating iNKT cells compared to the control group. Patients with severe disseminated blistering tended to display lower iNKT cell numbers than patients with moderate disease severity. In lesional skin of BP patients, an enrichment of iNKT cells was detected compared to skin biopsies from controls. Similarly to control biopsies, non-lesional biopsies of BP patients contained only few iNKT cells. In conclusion, the deficiency of circulating iNKT cells associated with enrichment at the site of cutaneous inflammation suggests that iNKT cells may play a pathophysiologically relevant role in BP.
Subject(s)
Natural Killer T-Cells/immunology , Pemphigoid, Bullous/blood , Skin/cytology , Adult , Aged , Aged, 80 and over , Biopsy , Case-Control Studies , Female , Flow Cytometry , Healthy Volunteers , Humans , Lymphocyte Count , Male , Middle Aged , Pemphigoid, Bullous/immunology , Pemphigoid, Bullous/pathology , Prospective Studies , Skin/immunology , Skin/pathology , Skin Neoplasms/blood , Skin Neoplasms/immunology , Skin Neoplasms/pathology , Young AdultABSTRACT
A male neonate was born with blisters on the trunk to a 37-year-old primigravid woman with a past medical history of recurrent, painful, topical steroid-responsive oral blisters. The diagnosis of neonatal pemphigus was made after the neonate and mother were found to have elevated desmoglein 3 (Dsg3) antibodies in conjunction with histopathologic features of pemphigus vulgaris. Interestingly, both neonate and mother also had elevated levels of BP180 antibodies, classically seen in bullous pemphigoid. This case is unique in that it portrays neonatal pemphigus, an already rare condition, complicated by the presence of BP180 antibodies.
Subject(s)
Autoantibodies/blood , Autoantigens/blood , Desmoglein 3/blood , Non-Fibrillar Collagens/blood , Pemphigus/immunology , Adult , Female , Humans , Infant, Newborn , Male , Pemphigoid, Bullous/blood , Pemphigoid, Bullous/immunology , Pemphigus/blood , Pemphigus/diagnosis , Collagen Type XVIIABSTRACT
BACKGROUND: Bullous pemphigoid (BP) has been associated with dipeptidyl peptidase-4 inhibitors (DPP4i). Clinical features, outcomes, and risk of BP for new DPP4i (linagliptin, saxagliptin, and alopgliptin) are not well established. Comparison of risk of BP appearance for DPP4i and other oral antidiabetic drugs (OADs) such as sodium glucose cotransporter 2 inhibitors has not been studied to date. OBJECTIVES: To describe the prevalence, sociodemographic, clinical, and histopathological characteristics, and outcome after drug withdrawal in DPP-4i-associated BP cases from our hospital. To review all Spanish spontaneous notifications of BP where DPP4i or OADs were included as suspected drugs and calculate the reporting odds ratios (RORs). METHODS: A retrospective observational study was performed examining the association between DDP4i and BP. Clinical features and RORs were analyzed. Data from the Spanish Pharmacovigilance System (SEFV) are included. RESULTS: In our center, 28 of 89 patients with BP (31.5%) were under DPP4i treatment; 53.6% were male, and mean age was 80.8 years. BP debuted the first year after DPP4i in 57.2%. BP control was achieved within 3.7 months after drug withdrawal. Regarding SEFV, 22 of 972 spontaneous notifications were related to BP and DPP4i. RORs were superior for DPP4i compared to other OADs. Vildagliptin had the highest ROR. CONCLUSIONS: We present the largest DPP4i-induced BP case series in a single center, with a detailed study of the sociodemographic, clinical, and histopathological characteristics of each patient, and their treatment and outcome. Vildagliptin had the highest risk. DPP4i-associated BP does not seem to have specific clinical characteristics.