ABSTRACT
CASPT2//CASSCF/6-31G photochemical reaction path computations for two 4-cis-nona-2,4,6,8-tetraeniminium cation derivatives, with the 4-cis double bond embedded in a seven- and eight-member ring, are carried out to model the reactivity of the corresponding ring-locked retinal chromophores. The comparison of the excited state branches of the two reaction paths with that of the native chromophore, is used to unveil the factors responsible for the remarkably short (60 fs) excited state (S(1)) lifetime observed when an artificial rhodopsin containing an eight member ring-locked retinal is photoexcited. Indeed, it is shown that the strain imposed by the eight-member ring on the chromophore backbone leads to a dramatic change in the shape of the S(1) energy surface. Our models are also used to investigate the nature of the primary photoproducts observed in different artificial rhodopsins. It is seen that only the eight member ring-locked retinal model can access a shallow energy minimum on the ground state. This result implies that the primary, photorhodopsin-like, transient observed in artificial rhodopsins could correspond to a shallow excited state minimum. Similarly, the second, bathorhodopsin-like, transient species could be assigned to a ground state structure displaying a nearly all-trans conformation.
Subject(s)
Biomimetic Materials/chemistry , Retinaldehyde/chemistry , Rhodopsin/analogs & derivatives , Isomerism , Models, Molecular , Molecular Conformation , Photochemistry , Quantum Theory , Rhodopsin/chemistry , Schiff Bases/chemistry , ThermodynamicsABSTRACT
The recovery of rod responsiveness after saturating flashes is greatly retarded above a certain critical level of rhodopsin bleaching (approximately 0.1%). A mathematical description of the process of turn-off of the phototransduction cascade allows attributing different phases of the recovery to specific products of rhodopsin photolysis. The fast phase is determined by quenching of metarhodopsin II and activated transducin. The slow phase is controlled by decay of partially inactivated (phosphorylated and arrestin-bound) metarhodopsins, and by regeneration of rhodopsin. The transition between the two regimes of adaptation is rather abrupt, occurring within a few-fold range of stimulus intensity. This marks the border between reversal of light adaptation and dark adaptation, as it is commonly defined.
Subject(s)
Dark Adaptation/physiology , Retinal Rod Photoreceptor Cells/physiology , Rhodopsin/analogs & derivatives , Adaptation, Ocular/physiology , Animals , Cyclic GMP/physiology , Photic Stimulation/methods , Rana ridibunda , Rhodopsin/physiology , Vision, Ocular/physiologyABSTRACT
In this study, we address the mechanism of visual arrestin release from light-activated rhodopsin using fluorescently labeled arrestin mutants. We find that two mutants, I72C and S251C, when labeled with the small, solvent-sensitive fluorophore monobromobimane, exhibit spectral changes only upon binding light-activated, phosphorylated rhodopsin. Our analysis indicates that these changes are probably due to a burying of the probes at these sites in the rhodopsin-arrestin or phospholipid-arrestin interface. Using a fluorescence approach based on this observation, we demonstrate that arrestin and retinal release are linked and are described by similar activation energies. However, at physiological temperatures, we find that arrestin slows the rate of retinal release approximately 2-fold and abolishes the pH dependence of retinal release. Using fluorescence, EPR, and biochemical approaches, we also find intriguing evidence that arrestin binds to a post-Meta II photodecay product, possibly Meta III. We speculate that arrestin regulates levels of free retinal in the rod cell to help limit the formation of damaging oxidative retinal adducts. Such adducts may contribute to diseases like atrophic age-related macular degeneration (AMD). Thus, arrestin may serve to both attenuate rhodopsin signaling and protect the cell from excessive retinal levels under bright light conditions.
Subject(s)
Arrestin/metabolism , Rhodopsin/analogs & derivatives , Rhodopsin/metabolism , Animals , Arrestin/genetics , Cattle , Fluorescent Dyes , Hydrogen-Ion Concentration , Kinetics , Mutation, Missense , Protein Binding , Protein Interaction Mapping , Retinaldehyde/metabolism , Rod Cell Outer Segment/chemistryABSTRACT
Low-temperature spectrophotometry was used to study the primary stages of rhodopsin photolysis. A digitonin extract of rhodopsin was irradiated at -155 degrees C with blue light of wavelength 436 nm. The stage of the bathorhodopsin --> lumirhodopsin conversion was accompanied by the simultaneous formation of several products. Formation of an intermediate product spectrally similar to the known "blue-shifted intermediate" (BSI) was demonstrated. It is suggested that the appearance of more than one intermediate product at each stage of photolysis reflects the existence of several conformational states of the rhodopsin molecule during its photoconversion.
Subject(s)
Photolysis , Retinal Pigments , Rhodopsin/analogs & derivatives , Rhodopsin/metabolism , Animals , Cattle , Freezing , Models, Biological , Photochemistry/methods , Retinal Pigments/chemistry , Retinal Pigments/radiation effects , Rhodopsin/chemistry , Spectrophotometry/methodsABSTRACT
Sterical constraints of the 9-methyl-binding pocket of the rhodopsin chromophore are probed using retinal analogues carrying substituents of increasing size at the 9 position (H, F, Cl, Br, CH(3), and I). The corresponding 11-Z retinals were employed to investigate formation of photosensitive pigment, and the primary photoproduct was identified by Fourier transform infrared difference spectroscopy. In addition, any effects of cumulative strain were studied by introduction of the 9-Z configuration and/or the alpha-retinal ring structure. Our results show that the 9-F analogue still can escape from the 9-methyl-binding pocket and that its photochemistry behaves very similar to the 9-demethyl analogue. The 9-Cl and 9-Br analogues behave very similar to the native 9-methyl pigments, but the 9-I retinal does not fit very well and shows poor pigment formation. This puts an upper limit on the radial dimension of the 9-methyl pocket at 0.45-0.50 nm. Introduction of the alpha-retinal ring constraint in the 11-Z series results in cumulative strain, because the 9-I and 9-Br derivatives cannot bind to generate a photopigment. The 9-Z configuration can partially compensate for the additional alpha-retinal strain. The corresponding 9-Br analogue does form a photopigment, and the other derivatives give increased photopigment yields compared to the corresponding 11-Z derivatives. In fact, 9-Z-alpha-retinal would be an interesting candidate for retinal supplementation studies. Our data provide direct support for the concept that the 9-methyl group is an important determinant in ligand anchoring and activation of the protein and in general agree with a three-point interaction model involving the ring, 9-methyl group, and aldehyde function.
Subject(s)
Halogens/metabolism , Retinaldehyde/analogs & derivatives , Retinaldehyde/chemical synthesis , Retinaldehyde/metabolism , Rhodopsin/analogs & derivatives , Rhodopsin/chemical synthesis , Rhodopsin/metabolism , Animals , Binding Sites , Cattle , Freezing , Isomerism , Ligands , Protein Binding , Rhodopsin/chemistry , Rod Opsins/chemistry , Rod Opsins/metabolism , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , Stereoisomerism , Structure-Activity RelationshipABSTRACT
Rhodopsin, the pigment protein responsible for dim-light vision, is a G protein-coupled receptor that converts light absorption into the activation of a G protein, transducin, to initiate the visual response. We have crystallised detergent-solubilised bovine rhodopsin in the native form and after chemical modifications as needles 10-40 microm in cross-section. The crystals belong to the trigonal space group P3(1), with two molecules of rhodopsin per asymmetric unit, related by a non-crystallographic 2-fold axis parallel with the crystallographic screw axis along c (needle axis). The unit cell dimensions are a=103.8 A, c=76.6 A for native rhodopsin, but vary over a wide range after heavy atom derivatisation, with a between 101.5 A and 113.9 A, and c between 76.6 A and 79.2 A. Rhodopsin molecules are packed with the bundle of transmembrane helices tilted from the c-axis by about 100 degrees . The two molecules in the asymmetric unit form contacts along the entire length of their transmembrane helices 5 in an antiparallel orientation, and they are stacked along the needle axis according to the 3-fold screw symmetry. Hence hydrophobic contacts are prominent at protein interfaces both along and normal to the needle axis. The best crystals of native rhodopsin in this crystal form diffracted X-rays from a microfocused synchrotron source to 2.55 A maximum resolution. We describe steps taken to extend the diffraction limit from about 10 A to 2.6 A.
Subject(s)
Metals, Heavy/chemistry , Rhodopsin/chemistry , Animals , Cattle , Crystallization , Crystallography, X-Ray , Detergents , Metals, Heavy/metabolism , Protein Structure, Tertiary , Retina/chemistry , Retina/metabolism , Rhodopsin/analogs & derivatives , Rhodopsin/isolation & purification , Rhodopsin/metabolism , SpectrophotometryABSTRACT
Rho (rhodopsin; opsin plus 11-cis-retinal) is a prototypical G protein-coupled receptor responsible for the capture of a photon in retinal photoreceptor cells. A large number of mutations in the opsin gene associated with autosomal dominant retinitis pigmentosa have been identified. The naturally occurring T4R opsin mutation in the English mastiff dog leads to a progressive retinal degeneration that closely resembles human retinitis pigmentosa caused by the T4K mutation in the opsin gene. Using genetic approaches and biochemical assays, we explored the properties of the T4R mutant protein. Employing immunoaffinity-purified Rho from affected RHO(T4R/T4R) dog retina, we found that the mutation abolished glycosylation at Asn(2), whereas glycosylation at Asn(15) was unaffected, and the mutant opsin localized normally to the rod outer segments. Moreover, we found that T4R Rho(*) lost its chromophore faster as measured by the decay of meta-rhodopsin II and that it was less resistant to heat denaturation. Detergent-solubilized T4R opsin regenerated poorly and interacted abnormally with the G protein transducin (G(t)). Structurally, the mutation affected mainly the "plug" at the intradiscal (extracellular) side of Rho, which is possibly responsible for protecting the chromophore from the access of bulk water. The T4R mutation may represent a novel molecular mechanism of degeneration where the unliganded form of the mutant opsin exerts a detrimental effect by losing its structural integrity.
Subject(s)
Mutation , Receptors, G-Protein-Coupled/chemistry , Rhodopsin/analogs & derivatives , Rod Opsins/genetics , Alleles , Amino Acid Sequence , Animals , Chromatography, Liquid , Cytoplasm/metabolism , Detergents/pharmacology , Disease Models, Animal , Dogs , Electrophoresis, Polyacrylamide Gel , Glycosylation , Immunoblotting , Immunohistochemistry , Ligands , Light , Mass Spectrometry , Models, Molecular , Molecular Sequence Data , Peptides/chemistry , Protein Structure, Tertiary , Proteins/chemistry , Receptors, G-Protein-Coupled/metabolism , Retina/pathology , Retinitis Pigmentosa/genetics , Retinoids/metabolism , Rhodopsin/chemistry , Rod Cell Outer Segment , Rod Opsins/metabolism , Time Factors , Ultraviolet Rays , rho GTP-Binding Proteins/genetics , rho GTP-Binding Proteins/metabolismABSTRACT
Photoactivation of rhodopsin yields a photointermediate, metarhodopsin I, during the formation of the fully activated photointermediate, metarhodopsin II. It is proposed that Glu181 and Ser186, in the second extracellular loop, play important roles in the stabilization of the protonated Schiff base of metarhodopsin I. Glu181 and Ser186 form a network of hydrogen bonds mediated by a water molecule in the dark-state crystal structure of rhodopsin. On the other hand, the counter-ion of the protonated Schiff base, Glu113, is not involved in the hydrogen-bond network, as it is located further than hydrogen-bond distance from Ser186. Herein, the conformations and proton arrangements of the protonated form of Glu181 and Ser186 in the hydrogen-bond network have been investigated by molecular-dynamics calculations of the rhodopsin crystal structure as well as in the structural model of metarhodopsin I. In the metarhodopsin I model, Ser186 mediated the hydrogen-bond network between Glu113 and Glu181, changing the protein's conformation and creating a space by the outward motion of transmembrane segment 3, while the hydroxyl group of Glu181 was favored in the hydrogen-bond network. The hydrogen bond between Glu113 and Ser186 is thought to reduce the basicity of the carboxylate of Glu113, maintaining the protonated state of the Schiff base in the metarhodopsin I state. In the Glu181Gln mutant, the hydroxyl group of Ser186 favored the water molecule as a proton donor in the metarhodopsin I state, since the carbonyl group of the Gln residue was favored in the hydrogen-bond network. These results indicate that the Gln181 residue interferes with the hydrogen-bond between Glu113 and Ser186 in the metarhodopsin I state, facilitating the neutralization of the protonated Schiff base.
Subject(s)
Glutamic Acid/chemistry , Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Serine/chemistry , Crystallography, X-Ray , Hydrogen Bonding , Models, Molecular , Photochemistry , Protein Conformation , Schiff Bases/chemistryABSTRACT
The role of glutamic acid 181 in the bovine rhodopsin retinylidene chromophore pocket was studied by expressing E181 mutants in COS cells and measuring, as a function of time, the absorbance changes produced after excitation of lauryl maltoside pigment suspensions with 7 ns laser pulses. All mutants studied except E181D showed accelerated decay of bathorhodopsin compared to wild type. Even for E181D, an anomalously large blue shift was observed in the absorption spectrum of the bathorhodopsin decay product, BSI. These observations support the idea that E181 plays a significant role in the earliest stages of receptor activation. E181 mutations have a pronounced effect on the decay of the lumirhodopsin photointermediate, primarily affecting the size of the red shift that occurs in the lumirhodopsin I to lumirhodopsin II transition that takes place on the 10 micros time scale after wild-type photoexcitation. While the spectral change that occurs in the lumirhodopsin I to lumirhodopsin II transition in wild-type rhodopsin is very small ( approximately 2 nm), making it difficult to detect, it is larger in E181D ( approximately 6 nm), making it evident even in the lower signal-to-noise ratio measurements possible with rhodopsin mutants. The change seen is even larger for the E181F mutant where significant amounts of a deprotonated Schiff base intermediate are produced with the 10 micros time constant of lumirhodopsin II formation. The E181Q mutant shows lumirhodopsin decay more similar to wild-type behavior, and no lumirhodopsin I to lumirhodopsin II transition can be resolved. The addition of chloride ion to E181Q increases the lumirhodopsin I-lumirhodopsin II spectral shift and slows the deprotonation of the Schiff base. The latter result is consistent with the idea that a negative charge at position 181 contributes to protonated Schiff base stability in the later intermediates.
Subject(s)
Glutamic Acid/genetics , Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Rhodopsin/genetics , Amino Acid Substitution/genetics , Animals , Aspartic Acid/genetics , COS Cells , Cattle , Glutamic Acid/chemistry , Glutamine/genetics , Mutagenesis, Site-Directed , Phenylalanine/genetics , Photolysis , Protons , Schiff Bases , SpectrophotometryABSTRACT
Rhodopsin bears 11-cis-retinal covalently bound by a protonated Schiff base linkage. 11-cis/all-trans isomerization, induced by absorption of green light, leads to active metarhodopsin II, in which the Schiff base is intact but deprotonated. The subsequent metabolic retinoid cycle starts with Schiff base hydrolysis and release of photolyzed all-trans-retinal from the active site and ends with the uptake of fresh 11-cis-retinal. To probe chromophore-protein interaction in the active state, we have studied the effects of blue light absorption on metarhodopsin II using infrared and time-resolved UV-visible spectroscopy. A light-induced shortcut of the retinoid cycle, as it occurs in other retinal proteins, is not observed. The predominantly formed illumination product contains all-trans-retinal, although the spectra reflect Schiff base reprotonation and protein deactivation. By its kinetics of formation and decay, its low temperature photointermediates, and its interaction with transducin, this illumination product is identified as metarhodopsin III. This species is known to bind all-trans-retinal via a reprotonated Schiff base and forms normally in parallel to retinal release. We find that its generation by light absorption is only achieved when starting from active metarhodopsin II and is not found with any of its precursors, including metarhodopsin I. Based on the finding of others that metarhodopsin III binds retinal in all-trans-C(15)-syn configuration, we can now conclude that light-induced formation of metarhodopsin III operates by Schiff base isomerization ("second switch"). Our reaction model assumes steric hindrance of the retinal polyene chain in the active conformation, thus preventing central double bond isomerization.
Subject(s)
Light , Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Schiff Bases/chemistry , Animals , Cattle , Deuterium/chemistry , Isomerism , Photochemistry , Protein Conformation , Retinaldehyde/chemistry , Retinaldehyde/metabolism , Rhodopsin/metabolism , Schiff Bases/metabolism , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , TemperatureABSTRACT
In the phototransduction pathway of rhodopsin, the metarhodopsin (Meta) III retinal storage form arises from the active G-protein binding Meta II by a slow spontaneous reaction through the Meta I precursor or by light absorption and photoisomerization, respectively. Meta III is a side product of the Meta II decay path and holds its retinal in the original binding site, with the Schiff base bond to the apoprotein reprotonated as in the dark ground state. It thus keeps the retinal away from the regeneration pathway in which the photolyzed all-trans-retinal is released. This study was motivated by our recent observation that Meta III remains stable for hours in membranes devoid of regulatory proteins, whereas it decays much more rapidly in situ. We have now explored the possibility of regulated formation and decay of Meta III, using intrinsic opsin tryptophan fluorescence and UV-visible and Fourier transform infrared spectroscopy. We find that a rapid return of Meta III into the regeneration pathway is triggered by the G-protein transducin (G(t)). Depletion of the retinal storage is initiated by a novel direct bimolecular interaction of G(t) with Meta III, which was previously considered inactive. G(t) thereby induces the transition of Meta III into Meta II, so that the retinylidene bond to the apoprotein can be hydrolyzed, and the retinal can participate again in the normal retinoid cycle. Beyond the potential significance for retinoid metabolism, this may provide the first example of a G-protein-catalyzed conversion of a receptor.
Subject(s)
Rhodopsin/analogs & derivatives , Rhodopsin/metabolism , Transducin/metabolism , Animals , Cattle , Cell Membrane/metabolism , Guanosine Diphosphate/metabolism , Hydrogen-Ion Concentration , Light , Peptides/metabolism , Retinal Rod Photoreceptor Cells/chemistry , Retinal Rod Photoreceptor Cells/cytology , Retinal Rod Photoreceptor Cells/metabolism , Rhodopsin/chemistry , Spectrophotometry, Ultraviolet , Spectroscopy, Fourier Transform Infrared , TemperatureABSTRACT
Meta III is an inactive intermediate thermally formed following light activation of the visual pigment rhodopsin. It is produced from the Meta I/Meta II photoproduct equilibrium of rhodopsin by a thermal isomerization of the protonated Schiff base C=N bond of Meta I, and its chromophore configuration is therefore all-trans 15-syn. In contrast to the dark state of rhodopsin, which catalyzes exclusively the cis to trans isomerization of the C11=C12 bond of its 11-cis 15-anti chromophore, Meta III does not acquire this photoreaction specificity. Instead, it allows for light-dependent syn to anti isomerization of the C15=N bond of the protonated Schiff base, yielding Meta II, and for trans to cis isomerizations of C11=C12 and C9=C10 of the retinal polyene, as shown by FTIR spectroscopy. The 11-cis and 9-cis 15-syn isomers produced by the latter two reactions are not stable, decaying on the time scale of few seconds to dark state rhodopsin and isorhodopsin by thermal C15=N isomerization, as indicated by time-resolved FTIR methods. Flash photolysis of Meta III produces therefore Meta II, dark state rhodopsin, and isorhodopsin. Under continuous illumination, the latter two (or its unstable precursors) are converted as well to Meta II by presumably two different mechanisms.
Subject(s)
Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Animals , Cattle , Darkness , Isomerism , Light , Photolysis , Protein Isoforms/chemistry , Protons , Schiff Bases/chemistry , Spectroscopy, Fourier Transform Infrared/methods , Spectrum Analysis, Raman , TemperatureABSTRACT
The vibrational structure of the chromophore in the primary photoproduct of vision, bathorhodopsin, is examined to determine the cause of the anomalously decoupled and intense C(11)=C(12) hydrogen-out-of-plane (HOOP) wagging modes and their relation to energy storage in the primary photoproduct. Low-temperature (77 K) resonance Raman spectra of Glu181 and Ser186 mutants of bovine rhodopsin reveal only mild mutagenic perturbations of the photoproduct spectrum suggesting that dipolar, electrostatic, or steric interactions with these residues do not cause the HOOP mode frequencies and intensities. Density functional theory calculations are performed to investigate the effect of geometric distortion on the HOOP coupling. The decoupled HOOP modes can be simulated by imposing approximately 40 degrees twists in the same direction about the C(11)=C(12) and C(12)-C(13) bonds. Sequence comparison and examination of the binding site suggests that these distortions are caused by three constraints consisting of an electrostatic anchor between the protonated Schiff base and the Glu113 counterion, as well as steric interactions of the 9- and 13-methyl groups with surrounding residues. This distortion stores light energy that is used to drive the subsequent protein conformational changes that activate rhodopsin.
Subject(s)
Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Rhodopsin/metabolism , Animals , Cattle , Freezing , Glutamic Acid/genetics , Light , Models, Molecular , Mutagenesis, Site-Directed , Photochemistry , Protein Conformation , Protein Structure, Secondary , Protons , Rhodopsin/genetics , Schiff Bases/chemistry , Serine/genetics , Species Specificity , Spectrum Analysis, Raman/methods , Static Electricity , ThermodynamicsABSTRACT
Activation of the visual pigment rhodopsin is caused by 11-cis to -trans isomerization of its retinal chromophore. High-resolution solid-state NMR measurements on both rhodopsin and the metarhodopsin II intermediate show how retinal isomerization disrupts helix interactions that lock the receptor off in the dark. We made 2D dipolar-assisted rotational resonance NMR measurements between (13)C-labels on the retinal chromophore and specific (13)C-labels on tyrosine, glycine, serine, and threonine in the retinal binding site of rhodopsin. The essential aspects of the isomerization trajectory are a large rotation of the C20 methyl group toward extracellular loop 2 and a 4- to 5-A translation of the retinal chromophore toward transmembrane helix 5. The retinal-protein contacts observed in the active metarhodopsin II intermediate suggest a general activation mechanism for class A G protein-coupled receptors involving coupled motion of transmembrane helices 5, 6, and 7.
Subject(s)
Retinaldehyde/chemistry , Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Binding Sites , Isomerism , Nuclear Magnetic Resonance, Biomolecular , Protein Structure, Secondary , Rhodopsin/metabolismABSTRACT
Pharaonis phoborhodopsin (ppR, or pharaonis sensory rhodopsin II, NpsRII) is a sensor for the negative phototaxis of Natronomonas (Natronobacterium) pharaonis. Arginine 72 of ppR corresponds to Arg-82 of bacteriorhodopsin, which is a highly conserved residue among microbial rhodopsins. Using various Arg-72 ppR mutants, we obtained the following results: 1). Arg-72(ppR) together possibly with Asp-193 influenced the pK(a) of the counterion of the protonated Schiff base. 2). The M-rise became approximately four times faster than the wild-type. 3). Illumination causes proton uptake and release, and the pH profiles of the sequence of these two proton movements were different between R72A mutant and the wild-type; it is inferred that Arg-72 connects the proton transfer events occurring at both the Schiff base and an extracellular proton-releasing residue (Asp-193). 4). The M-decays of Arg-72 mutants were faster ( approximately 8-27 folds at pH 8 depending on mutants) than the wild-type, implying that the guanidinium prevents the proton transfer from the extracellular space to the deprotonated Schiff base. 5), The proton-pumping activities were decreased for mutants having increased M-decay rates, but the extent of the decrease was smaller than expected. The role of Arg-72 of ppR on the photochemistry was discussed.
Subject(s)
Arginine/chemistry , Rhodopsin/analogs & derivatives , Rhodopsin/metabolism , Bacteriorhodopsins/chemistry , Chlorine/chemistry , Guanidine/chemistry , Hydrogen-Ion Concentration , Ions , Light , Mutation , Phosphatidylcholines/chemistry , Photochemistry , Photolysis , Proton Pumps , Protons , Recombinant Proteins/chemistry , Time FactorsABSTRACT
G-protein coupled receptors (GPCRs) mediate responses to many types of extracellular signals. So far, bovine rhodopsin, the inactive form of a GPCR, is the only member of the family whose three dimensional structure has been determined. It would be desirable to determine the structure of the active form of a GPCR. In this paper, we report the large scale preparation of a stable, homogenous species, truncated octopus rhodopsin (t-rhodopsin) in which proteolysis has removed the proline-rich C-terminal; this species retains the spectral properties and the ability for light-induced G-protein activation of unproteolyzed octopus rhodopsin. Moreover, starting from this species we can prepare a pure, active form of pigment, octopus t-Acid Metarhodopsin which has an all-trans-retinal as its agonist. Photoisomerization of t-Acid Metarhodopsin leads back to the inactive form, t-rhodopsin with the inverse agonist 11-cis-retinal. Octopus t-Acid Metarhodopsin can activate an endogenous octopus G-protein in the dark and this activity is reduced by irradiation with orange light which photoregenerates t-Acid Metarhodopsin back to the initial species, t-rhodopsin.
Subject(s)
Octopodiformes/chemistry , Receptors, G-Protein-Coupled/isolation & purification , Rhodopsin/analogs & derivatives , Rhodopsin/isolation & purification , Animals , Chromatography, High Pressure Liquid , Electrophoresis, Polyacrylamide Gel , Guanosine 5'-O-(3-Thiotriphosphate)/metabolism , Immunoblotting , Receptors, G-Protein-Coupled/metabolism , Retinaldehyde/metabolism , Rhodopsin/metabolism , Signal Transduction/physiology , SpectrophotometryABSTRACT
Model building of the two photointermediates, lumirhodopsin and metarhodopsin I, and the activated form of rhodopsin, metarhodopsin II, is described. An outward swing of the C-terminal portion of transmembrane segment 3, pivoting on Cys110 at the N-terminal end of transmembrane segment 3, led to structural models of lumirhodopsin and metarhodopsin I. The conformation of the chromophore in the lumirhodopsin and metarhodopsin I models is controlled by the motion of transmembrane segment 3 and agreed closely with the hydrogen-bonding states of the protonated Schiff base in lumirhodopsin and metarhodopsin I as deduced from their FTIR and resonance Raman spectra and with the negative and positive CD bands of lumirhodopsin and metarhodopsin I, respectively. The structure of metarhodopsin II was constructed by an outward swing of transmembrane segment 3 and the rigid-body motion of transmembrane segment 6. The arrangement of the entire transmembrane segment of the metarhodopsin II model closely agreed with the electron paramagnetic resonance spectra of spin-labeled rhodopsin mutants and provided a structural basis for the protonation of Glu134, which is a key process in transducin activation.
Subject(s)
Models, Molecular , Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Animals , Humans , Hydrogen Bonding , Molecular Structure , Motion , Photochemistry , Protein Conformation , Schiff BasesABSTRACT
Artificial visual pigment formation was studied by using 8-methyl-substituted retinals in an effort to understand the effect that alkyl substitution of the chromophore side chain has on the visual cycle. The stereoselective synthesis of the 9-cis and 11-cis isomers of 8-methylretinal, as well as the 5-demethylated analogues is also described. The key bond formations consist of a thallium-accelerated Suzuki cross-coupling reaction between cyclohexenylboronic acids and dienyliodides (C6-C7), and a highly stereocontrolled Horner-Wadsworth-Emmons or Wittig condensation (C11-C12). The cyclohexenylboronic acid was prepared by trapping the precursor cyclohexenyllithium species with B(OiPr)(3) or B(OMe)(3). The cyclohexenyllithium species is itself obtained by nBuLi-induced elimination of a trisylhydrazone (Shapiro reaction), or depending upon the steric hindrance of the ring, by iodine-metal exchange. In binding experiments with the apoprotein opsin, only 9-cis-5-demethyl-8-methylretinal yielded an artificial pigment; 9-cis-8-methylretinal simply provided residual binding, while evidence of artificial pigment formation was not found for the 11-cis analogues. Molecular-mechanics-based docking simulations with the crystal structure of rhodopsin have allowed us to rationalize the lack of binding displayed by the 11-cis analogues. Our results indicate that these isomers are highly strained, especially when bound, due to steric clashes with the receptor, and that these interactions are undoubtedly alleviated when 9-cis-5-demethyl-8-methylretinal binds opsin.
Subject(s)
Retinaldehyde/analogs & derivatives , Retinaldehyde/chemistry , Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Binding Sites , Models, Chemical , Retinaldehyde/chemical synthesis , Rhodopsin/chemical synthesis , StereoisomerismABSTRACT
High-resolution solid-state NMR methods have been used to analyze the conformation of the chromophore in the late photointermediate metarhodopsin-I, from observation of (13)C nuclei introduced into the beta-ionone ring (at the C16, C17, and C18 methyl groups) and into the adjoining segment of the polyene chain (at C8). Bovine rhodopsin in its native membrane was also regenerated with retinal that was (13)C-labeled close to the 11-Z bond (C20 methyl group) to provide a reporter for optimizing and quantifying the photoconversion to metarhodopsin-I. Indirect photoconversion via the primary intermediate, bathorhodopin, was adopted as the preferred method since approximately 44% conversion to the metarhodopsin-I component could be achieved, with only low levels (approximately 18%) of ground-state rhodopsin remaining. The additional photoproduct, isorhodopsin, was resolved in (13)C spectra from C8 in the chain, at levels of approximately 38%, and was shown using rotational resonance NMR to adopt the 6-s-cis conformation between the ring and the polyene chain. The C8 resonance was not shifted in the metarhodopsin-I spectral component but was strongly broadened, revealing that the local conformation had become less well defined in this segment of the chain. This line broadening slowed rotational resonance exchange with the C17 and C18 ring methyl groups but was accounted for to show that, despite the chain being more relaxed in metarhodopsin-I, its average conformation with respect to the ring was similar to that in the ground state protein. Conformational restraints are also retained for the C16 and C17 methyl groups on photoactivation, which, together with the largely preserved conformation in the chain, argues convincingly that the ring remains with strong contacts in its binding pocket prior to activation of the receptor. Previous conclusions based on photocrosslinking studies are considered in view of the current findings.
Subject(s)
Norisoprenoids/chemistry , Rhodopsin/analogs & derivatives , Rhodopsin/chemistry , Animals , Binding Sites , Carbon Isotopes , Cattle , Isomerism , Models, Molecular , Molecular Conformation , Norisoprenoids/radiation effects , Nuclear Magnetic Resonance, Biomolecular/methods , Photochemistry , Rhodopsin/radiation effectsABSTRACT
To regenerate light-sensitive rhodopsin in rods from active metarhodopsin II (Meta II), all-trans-retinal must be removed from the retinal binding pocket and metabolically supplied 11-cis-retinal has to form a new retinylidene bond in the active site. Recent work from this laboratory has focused on Meta II decay and release and uptake of retinals in opsin employing intrinsic protein fluorescence. Here we summarize the results in the retinal channeling hypothesis, which describes a passage of the chromophore through the protein. 11-cis-retinal is taken up into an entrance site, and photolyzed all-trans-retinal is released from the active site into an exit site.