RESUMO
The fruits of Pistacia khinjuk Stocks were collected from Ilam province, Iran. The aim of this study was to analyse antioxidant capacity and phenolic composition of different parts of P. khinjuk fruit. The antioxidant capacity of extracts was measured using different assays: ferric reducing ability of plasma, 2,2-diphenyl-1-picrylhydrazyl and nitric oxide radical scavenging. The phenolic composition of P. khinjuk fruit is reported for the first time. Amongst different parts of the fruit analysed in this study, hull extract contained the highest total phenolic and flavonoid contents. We observed a high correlation between different antioxidant activity and total phenolic and flavonoid contents. Therefore, antioxidant capacity can be related to total phenolic and flavonoid contents. A correlation analysis revealed that ascorbic acid, gallic acid, rutin, caffeic acid, ferulic acid and sinapic acid were the phenolic compounds mainly responsible for antioxidant power of the fruit extracts.
Assuntos
Antioxidantes/química , Antioxidantes/farmacologia , Fenóis/análise , Pistacia/química , Compostos de Bifenilo/química , Compostos de Bifenilo/metabolismo , Ácidos Cafeicos/análise , Ácidos Cafeicos/farmacologia , Cromatografia Líquida de Alta Pressão , Avaliação Pré-Clínica de Medicamentos , Flavonoides/análise , Flavonoides/química , Frutas/química , Ácido Gálico/análise , Ácido Gálico/farmacologia , Irã (Geográfico) , Óxido Nítrico/metabolismo , Fenóis/química , Fenóis/farmacologia , Picratos/química , Picratos/metabolismo , Rutina/análise , Rutina/farmacologiaRESUMO
Leaves of 10 Bene genotypes were collected from six provinces (West Azerbaijan, Kurdistan, Kermanshah, Ilam, Hamedan and Lorestan) in Iran. This study was carried out to better characterise the total phenolic and flavonoid contents, antioxidant capacity and phenolic composition, as well as to evaluate the correlation between content of phenolic compounds and antioxidant activity in 10 genotypes of Bene. The antioxidant activity of leaf extracts was measured using different assays: ferric reducing antioxidant power, nitric oxide radical scavenging and 2,2-diphenyl-1-picrylhydrazyl. The results indicated that the antioxidant capacity can be related to total phenolic and flavonoid content, so that among all the genotypes studied here, the highest and the lowest phenolic content and antioxidant activity were observed in B2 and B10 genotypes, respectively. Analysing the phenolic composition using high performance liquid chromatography, caffeic acid, vanillic acid, ferulic acid and sinapic acid were found in all investigated genotypes.
Assuntos
Antioxidantes/análise , Antioxidantes/farmacologia , Fenóis/análise , Fenóis/farmacologia , Pistacia/química , Extratos Vegetais/farmacologia , Compostos de Bifenilo , Cromatografia Líquida de Alta Pressão , Flavonoides/análise , Flavonoides/farmacologia , Genótipo , Irã (Geográfico) , Oxirredução , Picratos , Pistacia/genética , Extratos Vegetais/químicaRESUMO
Five genotypes of Bene were collected from West Azerbaijan, Kurdistan, Kermanshah and Ilam provinces, Iran. Total phenolic and flavonoid contents were determined and antioxidant activity of the extracts were measured using different assays: FRAP, nitric oxide radical scavenging and DPPH. Total phenolic and flavonoid contents of the hull extracts were significantly higher than shell and kernel extracts. In hull extracts, positive correlation coefficient was observed between total phenolic content and FRAP (R(2) = 0.98) and DPPH (R(2) = 0.66) assays. The results showed that, the highest antioxidant activity of hull extract may be attributed to higher total phenolic content. Analyzing the phenolic composition using HPLC, sinapic acid, vanillic acid and p-hydroxybenzoic acid were found in hull and shell extracts of all genotypes. Among all the genotypes studied here, B4 showed the highest phenolic content and antioxidant activity.