RESUMO
The mitochondria are critical mediators of cellular redox homeostasis due to their role in the generation and dissipation of reactive oxygen/nitrogen species (ROS/RNS). Modulations in ROS/RNS levels in the mitochondria are often reflected through oxidation/nitrosation of highly redox-sensitive cysteine residues within this organelle. Oxidation/nitrosation of functional cysteines on mitochondrial proteins serves to modulate protein activity, localization, and complexation in response to cellular stress, thereby controlling critical processes such as oxidative phosphorylation, apoptosis, and redox signalling. In this review, we describe mitochondrial sources of ROS/RNS, cysteine modifications that are triggered by increased mitochondrial ROS/RNS, and examples of key mitochondrial proteins that are regulated through cysteine-mediated redox signalling. We highlight recent advancements in proteomic methods to study cysteine posttranslational modifications. These tools will further aid in illuminating the important role of cysteine in maintaining and transducing redox signals in the mitochondria.
Assuntos
Cisteína/metabolismo , Mitocôndrias/metabolismo , Oxirredução , Transdução de Sinais , Estresse Oxidativo , Proteoma , Proteômica , Espécies Reativas de Nitrogênio/metabolismo , Espécies Reativas de Oxigênio/metabolismoRESUMO
Cystic fibrosis (CF) is one of the most common autosomal recessive diseases among Caucasians caused by a mutation in the CFTR gene. However, the clinical outcome of CF pulmonary disease varies remarkably even in patients with the same CFTR genotype. This has led to a search for genetic modifiers located outside the CFTR gene. The aim of this study was to evaluate the effect of functional variants in prostaglandin-endoperoxide synthase genes (COX1 and COX2) on the severity of lung disease in CF patients. To the best of our knowledge, it is the first time when analysis of COX1 and COX2 as potential CF modifiers is provided. The study included 94 CF patients homozygous for F508del mutation of CFTR. To compare their clinical condition, several parameters were recorded, e.g. a unique clinical score: disease severity status (DSS). To analyse the effect of non-CFTR genetic polymorphisms on the clinical course of CF patients, the whole coding region of COX1 and selected COX2 polymorphisms were analysed. Statistical analysis of genotype-phenotype associations revealed a relationship between the heterozygosity status of identified polymorphisms and better lung function. These results mainly concern COX2 polymorphisms: -765G>C and 8473T>C. The COX1 and COX2 polymorphisms reducing COX protein levels had a positive effect on all analysed clinical parameters. This suggests an important role of these genes as protective modifiers of pulmonary disease in CF patients, due to inhibition of arachidonic acid conversion into prostaglandins, which probably reduces the inflammatory process.
Assuntos
Ciclo-Oxigenase 1/genética , Ciclo-Oxigenase 2/genética , Fibrose Cística/enzimologia , Fibrose Cística/genética , Índice de Gravidade de Doença , Adolescente , Adulto , Criança , Pré-Escolar , Ciclo-Oxigenase 1/química , Ciclo-Oxigenase 1/metabolismo , Ciclo-Oxigenase 2/metabolismo , Fibrose Cística/patologia , Fibrose Cística/fisiopatologia , Regulador de Condutância Transmembrana em Fibrose Cística/genética , Feminino , Estudos de Associação Genética , Homozigoto , Humanos , Pulmão/enzimologia , Pulmão/patologia , Pulmão/fisiopatologia , Masculino , Estrutura Secundária de Proteína , Testes de Função Respiratória , Adulto JovemRESUMO
We investigate U(N) Chern-Simons theories on the noncommutative plane. We show that for the theories to be consistent quantum mechanically, the coefficient of the Chern-Simons term should be quantized kappa = n/2pi with an integer n. This is a surprise for the U(1) gauge theory. When uniform background charge density rho(e) is present, the quantization rule changes to kappa+rho(e)straight theta = n/2pi with the noncommutative parameter straight theta. With the exact expression for the angular momentum, we argue in the U(1) theory that charged particles in the symmetric phase carry fractional spin 1/2n and vortices in the broken phase carry half-integer or integer spin -n/2.
RESUMO
We consider the nonrelativistic field theory with a quartic interaction on a noncommutative plane and compute the 2-->2 scattering amplitude within perturbative analysis to all orders. We regain the results of the perturbative analysis by finding the scattering and the bound state wave functions of the two particle Schrodinger equation. These wave functions unusually have two center positions in the relative coordinates, whose separation is transverse to the total momentum and scales linearly with its magnitude, exhibiting the stringy nature of the noncommutative field theory.
RESUMO
A protease with a molecular mass of 48 kDa is secreted by the fire blight pathogen Erwinia amylovora in minimal medium. We characterized this activity as a metalloprotease, since the enzyme was inhibited by EDTA and o -phenanthroline. A gene cluster was determined to encode four genes connected to protease expression, including a structural gene (prtA) and three genes (prtD, prtE, prtF) for secretion of the protease, which are transcribed in the same direction. The organization of the protease gene cluster in E. amylovora is different from that in other Gram-negative bacteria, such as Erwinia chrysanthemi, Pseudomonas aeruginosa and Serratia marcescens. On the basis of the conservative motif of metalloproteases, PrtA was identified to be a member of the metzincin subfamily of zinc-binding metalloproteases, and was confirmed to be the 48 kDa protease on gels by sequencing of tryptic peptide fragments derived from the protein. The protease is apparently secreted into the external medium through the type I secretion pathway via PrtD, PrtE and PrtF which share more than 90% identity with the secretion apparatus for lipase of S. marcescens. A protease mutant was created by Tn 5 -insertions, and the mutation localized in the prtD gene. The lack of protease reduced colonization of an E. amylovora secretion mutant labelled with the gene for the green fluorescent protein (gfp) in the parenchyma of apple leaves.