Assuntos
Regulação da Expressão Gênica , Interferon-alfa/fisiologia , Interferon beta/biossíntese , Sindbis virus , Linhagem Celular Transformada , Efeito Citopatogênico Viral , Eletroforese em Gel de Ágar , Humanos , Interferon beta/genética , Reação em Cadeia da Polimerase , RNA Mensageiro/biossíntese , Transcrição GênicaRESUMO
Expression of 2,5-OAS and ds-protein kinase (ds-PK) in human fibroblast culture following treatment with alpha-IF in a dose of 1000 U or challenge with Karelian fever virus (KFV) (5 PFU/cell, 4 h.p.i.) was compared. A highly sensitive and rapid method, coupled RT-PCR, was used to detect mRNA transcription levels. Alpha-IF had an expressed stimulating effect on 2,5-OAS mRNA level, whereas the virus markedly inhibited the synthesis of ds-PK mRNA.
Assuntos
2',5'-Oligoadenilato Sintetase/genética , Regulação Enzimológica da Expressão Gênica , Interferon-alfa/farmacologia , Proteínas Serina-Treonina Quinases/genética , Sindbis virus/fisiologia , Fibroblastos/efeitos dos fármacos , Fibroblastos/enzimologia , Fibroblastos/virologia , Humanos , Reação em Cadeia da Polimerase , RNA Mensageiro/genética , Transcrição Gênica , eIF-2 QuinaseRESUMO
A recombination of the genomes of VEE and KF viruses, two alphaviruses of different serotypes, takes place in mixed infection of chick embryo fibroblasts and Vero cells with vaccine strain VEE-230 and KF-9298 strain. This recombination resulted in the production of recombinant virions with altered antigenic properties. Polymerase chain reaction (PCR) rapidly and effectively detected the hybrid sites of the genome in recombinant viruses. Short nucleotide sequences (approximately 20 n. p.) of a known primary structure in the RNA genomes, specifically reacting with the complementary PCR primers, were chosen as genetic markers. These data are the first experimental validation of the probable recombinant formation of alphaviruses in nature. They are in good agreement with findings of the recombinant structure of Western equine encephalomyelitis virus genome.
Assuntos
Alphavirus/genética , Vírus da Encefalite Equina Venezuelana/genética , Nucleocapsídeo/genética , RNA Viral/genética , Recombinação Genética , Proteínas do Envelope Viral/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Embrião de Galinha , Chlorocebus aethiops , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Células VeroRESUMO
The alcoholization of pregnant female rats (5 g/kg) results in a decrease of endogenous ethanol level in their offspring and distant disturbances of the conditioned reflex activities of the young rats deteriorating the formation and preservation of the skill with an emotional positive reinforcement. Sodium gamma-gydroxybutyrate administered in a dose of 50 mg/kg from the 8th to the 20th day of life prevents the above-mentioned disturbances of learning and memory, restores the level of endogenous ethanol, corrects the parameters of lipid and mediator metabolism in the brain and blood changed by prenatal alcoholization.
Assuntos
Alcoolismo/tratamento farmacológico , Comportamento Animal/efeitos dos fármacos , Encefalopatias/induzido quimicamente , Oxibato de Sódio/uso terapêutico , Alcoolismo/metabolismo , Animais , Aminas Biogênicas/metabolismo , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Encefalopatias/tratamento farmacológico , Encefalopatias/metabolismo , Avaliação Pré-Clínica de Medicamentos , Etanol/toxicidade , Feminino , Aprendizagem/efeitos dos fármacos , Metabolismo dos Lipídeos , Memória/efeitos dos fármacos , Gravidez , Efeitos Tardios da Exposição Pré-Natal , RatosRESUMO
A number of biochemical parameters have been determined which characterized the state of CNS and the structure of cell membranes in antenatally alcoholized animals. As compared with the control group the alcoholized animals had a significantly increased level of phosphatidyl inositol diphosphate in brain phospholipid fractions, while content of serotonin was reduced in their brain and increased in blood plasma; content of phosphatidyl serine and the cholesterol/phospholipids ratio were reduced in brain.