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In this work, a multiplexed colorimetric strategy was initiated for simultaneous and fast visualization of dyes using low-cost and easy-to-prepare indicator papers as sorbents. Response surface methodology (RSM) was employed to model statistically and optimize the process variables for dyes extraction and colorimetric assays. Multiplexed colorimetry was realized by virtue of synchronous color alignments from different dimensions of multiple dyes co-stained colorimetric cards under RSM-optimized conditions, and smartphone-based image analysis was subsequently performed from different modes to double-check the credibility of colorimetric assays. As concept-to-proof trials, simultaneous visualization of dyes in both beverages and simulated dye effluents was experimentally proved with results highly matched to HPLC or spiked amounts at RSM-predicted staining time as short as 50 s â¼3 min, giving LODs as low as 0.97 ± 0.22/0.18 ± 0.08 µg/mL (tartrazine/brilliant blue) for multiplexed colorimetry, which much lower than those obtained by single colorimetry. Since this is the first case to propose such a RSM-guided multiplexed colorimetric concept, it will provide a reference for engineering of other all-in-one devices which can realize synchronous visualization applications within limited experimental steps.
Assuntos
Colorimetria , Corantes , Smartphone , Colorimetria/métodos , Corantes/química , Corantes/análise , Contaminação de Alimentos/análise , Tartrazina/análise , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/química , Processamento de Imagem Assistida por Computador/métodos , Benzenossulfonatos/química , Bebidas/análiseRESUMO
Herein, a multi-mode visualization platform was initiated for in-situ detection of food dyes (FDs) by combining colorimetry, fluorometry and smartphonebased digital image analysis, in which water-dispersible quantum dots (QDs) were served as nanoprobes. Colorimetry was achieved by color comparison, while both fluorometry and fluorescence quantification were performed through inner filter effect (IFE)-induced fluorescence quenching, then color information (RGB & gray-scale values) of colorimetry and fluorometry was picked by a smartphone to reconstruct digitized alignments. Since IFE mechanism was concentration-dependent but did not rely on the interaction between fluorophore and quencher, the whole process of fluorescence response could be finished within 10 s, and both color gradients and fluorescence changes showed fine mappings to FDs concentrations in the range of 1.0 × 10-3â¼0.035 mg/mL for brilliant blue, and 1.0 × 10-4â¼0.1 mg/mL for Allura red and sunset yellow. As a proof-of-concept, the in-situ multi-mode visualization of these FDs in real beverages was experimentally proved to be highly feasible and reliable as compared with instrumental techniques like UV-vis/fluorescence spectrometry, along with HPLC. Finally, this strategy was extended to the multi-mode visualization of non-food dyes in three simulated wastewater samples with high credibility by contrast with the true additive amounts of model dyes.
Assuntos
Colorimetria , Pontos Quânticos , Smartphone , Fluorometria , Espectrometria de Fluorescência/métodos , Corantes Fluorescentes/química , Pontos Quânticos/química , Carbono/química , Limite de DetecçãoRESUMO
Background: Pediatric perianal fistula is a common disorder. It is more difficult to detect the fistula tract and internal opening (IO) in children than in adults. This study aimed to evaluate the clinical diagnostic value of transcutaneous perianal ultrasound for children with perianal fistula. Methods: A retrospective review was conducted by analysing the preoperative transcutaneous perianal ultrasound and intraoperative exploration results of 203 consecutive patients who were <3 years old and diagnosed with perianal fistula. Analyses were conducted to evaluate the accuracy and consistency of utilizing the transcutaneous perianal ultrasound in the diagnosis of the complexity and location of the IO of perianal fistulas. Results: Compared with intraoperative exploration, the preoperative transcutaneous perianal ultrasonography has almost perfect agreement (Kappa = 0.881, P < 0.001) in the diagnosis of fistula tract complexity and IO with a sensitivity of 92% and a specificity of 97%. In addition, both intraoperative exploration and transcutaneous perianal ultrasound diagnosis showed high consistency in the identification of the IO of perianal fistulas (Quadrant I Kappa = 0.831, Quadrant II Kappa = 0.773, Quadrant III Kappa = 0.735, Quadrant IV Kappa = 0.802, all P < 0.01). The IOs were mainly distributed in Quadrants IV and II in both simple and complex fistulas. Conclusions: Transcutaneous perianal ultrasound, as a non-invasive and simple imaging technique, showed high accuracy in the diagnosis and identification of the fistula classification and IO location. It could be considered a first-line diagnostic instrument for evaluating perianal fistulas among children.
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Herein, a strategy combining colorimetry and inner filter effect (IFE)-based fluorometry was developed for multimode visualization of food dyes (FDs) using CdTe quantum-dots-doped fluorescent indicator papers as a sample-to-answer device. Colorimetry was straightforwardly achieved by FDs extraction through electrostatic interaction and hydrophobic effect while fluorometry was implemented by IFE-induced fluorescence quenching. RGB/gray-scale values of colorimetry and fluorometry were furtherly picked by a smartphone application and applied to reconstruct color information-based digital image analysis for both direct alignments and linear regression analysis. The apparent color and fluorescence of FDs-bound indicator papers, together with their digitized color information, showed a good mapping to FDs concentrations in the range of 0-0.5 mg/mL for Sunset Yellow, 0-0.2 mg/mL for Allura Red, and 0-0.08 mg/mL for Brilliant Blue. As a proof of concept, the dosages of these FDs in real beverages and simulated dye effluents were deduced and cross-validated by different visualization modes, and finally double-checked by instrumental techniques such as spectrometric methods, high-performance liquid chromatography (HPLC), and mass spectroscopy (MS). The above findings concluded that (i) IFE mechanism is generally applicable to build fluorometric systems and (ii) cross validation of different visualization modes can markedly improve detection accuracy, which may provide references for design and fabrication of novel "lab-on-paper" devices for visualization applications with high reliability.
Assuntos
Compostos de Cádmio , Pontos Quânticos , Colorimetria , Reprodutibilidade dos Testes , Pontos Quânticos/química , Telúrio/química , Fluorometria , Corantes Fluorescentes/química , Carbono/químicaRESUMO
Cerebrospinal fluid (CSF) hypovolemia is the core of spontaneous intracranial hypotension (SIH). More than 1000 magnetic resonance myelography (MRM) images are required to evaluate each subject. An effective spinal CSF quantification method is needed. In this study, we proposed a cascade artificial intelligence (AI) model to automatically segment spinal CSF. From January 2014 to December 2019, patients with SIH and 12 healthy volunteers (HVs) were recruited. We evaluated the performance of AI models which combined object detection (YOLO v3) and semantic segmentation (U-net or U-net++). The network of performance was evaluated using intersection over union (IoU). The best AI model was used to quantify spinal CSF in patients. We obtained 25,603 slices of MRM images from 13 patients and 12 HVs. We divided the images into training, validation, and test datasets with a ratio of 4:1:5. The IoU of Cascade YOLO v3 plus U-net++ (0.9374) was the highest. Applying YOLO v3 plus U-net++ to another 13 SIH patients showed a significant decrease in the volume of spinal CSF measured (59.32 ± 10.94 mL) at disease onset compared to during their recovery stage (70.61 ± 15.31 mL). The cascade AI model provided a satisfactory performance with regard to the fully automatic segmentation of spinal CSF from MRM images. The spinal CSF volume obtained through its measurements could reflect a patient's clinical status.
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Flavonoids have many positive pharmacological properties, such as antioxidant, antitumor, and anti-inflammatory activities. However, factors such as low water solubility and low dissolution rate limit their use. To overcome their poor solubility, carrier-free apigenin (API) microparticles and nanoparticles were prepared using three types of antisolvent precipitation technologies: supercritical antisolvent (SCF) technology, ultrasonic-assisted liquid antisolvent (UAL) technology, and high-pressure homogenization (HPH) technology. All three technologies can produce uniform tiny particles. However, the API particles obtained using these different techniques show subtle differences in terms of physical and chemical properties and biological activity. The preparation, characterization, and potential use of API microparticles and nanoparticles to improve in vitro release were studied. The resulting API particles were investigated and compared using Fourier-transform infrared spectroscopy, differential scanning calorimetry, X-ray powder diffraction, and scanning electron microscopy. We determined the optimum conditions for SCF, UAL, and HPH technologies to produce API microparticles and nanoparticles. The antioxidant and antitumor properties of the API particles were also investigated. The results demonstrated that the reduced particle size of the APIs prepared via SCF, UAL, and HPH technologies contributed to the enhanced dissolution rate, which in turn enhanced API bioactivity.
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Apigenina , Nanopartículas , Antioxidantes , Apigenina/química , Varredura Diferencial de Calorimetria , Cristalização/métodos , Microscopia Eletrônica de Varredura , Nanopartículas/química , Tamanho da Partícula , Solubilidade , Solventes/química , Tecnologia , UltrassomRESUMO
Osteoarthritis (OA) is the most common form of arthritis in the adult population and is a leading cause of disability. OA-related genetic loci may play an important role in clinical diagnosis and disease progression. With the rapid development of diverse technologies and omics methods, many OA-related public data sets have been accumulated. Here, we retrieved a diverse set of omics experimental results from 159 publications, including genome-wide association study, differentially expressed genes and differential methylation regions, and 2405 classified OA-related gene markers. Meanwhile, based on recent single-cell RNA-seq data from different joints, 5459 cell-type gene markers of joints were collected. The information has been integrated into an online database named OAomics and molecular biomarkers (OAOB). The database (http://ibi.zju.edu.cn/oaobdb/) provides a web server for OA marker genes, omics features and so on. To our knowledge, this is the first database of molecular biomarkers for OA.
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Estudo de Associação Genômica Ampla , Osteoartrite , Bases de Dados Factuais , Marcadores Genéticos , Humanos , Osteoartrite/genéticaRESUMO
A filter paper-based colorimetric strategy for instrument-independent visual detection of artificial food colorants (FCs) was developed in this study. Indicator papers were prepared via the one-step polycondensation of silane coupling agents onto glass microfiber filter papers, and colorimetric cards with a fine one-to-one correspondence between their colors and FCs concentrations were straightforward obtained by the extraction of FCs with indicator papers by virtue of electrostatic interaction and hydrophobic effect. Filter papers post-modified via such a simple way were proved to be of improved binding class selectivity and colorimetric sensitivity, allowing for in-situ colorimetric assay of FCs in an unprecedently wide range of applicable pH (1.0-12.0) with high reliability and fine versatility. Finally, the semiquantitative naked-eye determination of FCs (Allura red, brilliant blue and sunset yellow) in real-world drink samples was experimentally confirmed to be feasible by comparison with the findings of UV-vis absorption spectra, HPLC and mass spectra.
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Corantes de Alimentos , Cromatografia Líquida de Alta Pressão , Colorimetria , Espectrometria de Massas , Reprodutibilidade dos TestesRESUMO
Secale strictum subsp. kuprijanovii is a perennial, hermaphrodite wild rye species and a progenitor of the modern cultivated rye, Secale cereale. With high adaptive capacity in stress conditions, it is valuable for enriching the germplasm resources of rye. Therefore, to elucidate its genetic and phylogenetic relationship is of great importance. We hereby sequenced, assembled and presented for the first time the complete chloroplast genome of this less studied species. The whole genome is 137,079 bp in size, including a large single copy region of 81,099 bp, a small single copy region of 12,820 bp and two separated inverted repeat regions of 43,160 bp. A total of 109 unique genes were annotated, including 67 protein-coding genes, 38 tRNA genes and 4 rRNA genes. Phylogenetic analysis showed that Secale strictum subsp. kuprijanovii clustered most closely with Secale cereal. A remarkably close evolutionary relationship of S.strictum subsp. kuprijanovii with various wheat varieties may indicate its usage as a genetic resource for the breeding of both the cultivated rye and wheat.
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Circular RNA (circRNA) is a kind of new regulatory RNA with diverse biological functions. Numerous circRNAs have been identified in many plant species; however, evolution of plant circRNAs remains largely unknown. In this study, we assembled full-length sequences of 6519 rice (Oryza sativa) circRNAs and analyzed their conservation in another 46 plant species based on comparison of sequences and expression patterns. We found that, at the genomic level, 8.7% of the 6519 circRNAs were conserved in dicotyledonous plants and 49.1% in Oryza genus. Meanwhile, 57.8% of parental protein-coding genes of the rice circRNAs originated recently after divergence of monocotyledonous plants, implying recent origin of the majority of rice circRNAs, a conclusion further supported by the results based on analysis of 4663 full-length circRNAs in Arabidopsis thaliana. Accordingly, we proposed three models to address the origination of different types of circRNAs. Taken together, the results obtained in this study provide new insights for the evolutionary dynamics of plant circRNAs and candidate circRNAs for further functional exploration.
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Oryza , RNA Circular , Oryza/genética , Plantas/genética , RNA/genética , Análise de Sequência de RNARESUMO
Myoglobin (Mb) is an ideal biochemical marker for the diagnosis of certain diseases caused by damage to heart muscle or skeletal muscle. Nevertheless, serum myoglobin levels are usually very low while the interference components in real sample are extremely abundent. Hence, it is of great clinical significance to establish an effective method for Mb targeting. To obtain desired selectivity, targeting biomolecules like antibody and aptamer are essential to 'the state of the art'. However, such biomolecules suffer from many disadvantages, such as hard to prepare, susceptible to protease degradation, and high cost. Thus, novel alternatives that can overcome these issues are highly desirable. Herein, we pioneered a template-anchored controllable surface imprinting strategy for selective extraction of Mb from human serum via combining with facile magnetic separation of magnetic nanoparticles (MNPs). Mb-imprinted MNPs, as antibody-biomimetic materials, were prepared using amino group-modified MNPs as substrates and water-soluble self-polymerizable dopamine as imprinting monomer. The optimized imprinting time was 70 min, giving an optimal performance with high practical imprinting efficiency (up to 41%), high imprinting factor (4.2), high binding affinity (Kd=(2.05 ± 0.09) × 10-5 M), as well as excellent recognition selectivity. Moreover, compared to bare MNPs, Mb-imprinted MNPs possessed markedly better pH tolerance. Finally, the selective extraction of Mb from human serum sample by Mb-imprinted MNPs was experimentally confirmed and the recoveries of Mb in spiked serum ranged from (91.12 ± 6.81)% to (107.99 ± 7.76)%, indicating that the Mb-imprinted MNPs could be competent for the selective analysis of Mb in real bio-samples like human serum with high precision and reliability.