ß-cyclodextrin microencapsulation enhanced antioxidant and antihyperlipidemic properties of Tunisian Periploca angustifolia roots condensed tannins in rats.

This study aimed to investigate the microencapsulation of novel condensed tannins isolated from Periploca angustifolia roots, using ß-cyclodextrin macrocyclic oligosaccharides, in order to enhance their antioxidant and antihyperlipidemic potentials. Scanning electron microscopy and Fourier transform infrared spectroscopy results revealed that tannin fraction was successfully included into ß-cyclodextrin cavities proved with an encapsulation efficacy of 70%. Our in vitro findings highlighted that both pure and encapsulated tannins have efficient inhibition capacities of pancreatic lipase activity. However, the inclusion complex has the greatest, in vivo, antioxidant, and antihyperlipidemic effects. In fact, results showed that complexed tannins had markedly restored serum lipid biomarkers, lipid peroxidation, protein carbonyl oxidation, and antioxidant enzyme defense. These findings were additionally confirmed by aortic and myocardial muscle sections of histological examination. Consequently, ß-cyclodextrin microencapsulation may be considered as an effective and promising technique for tannin delivery with improved antioxidant and antihyperlipidemic activities.

Optimization, isolation, characterization and hepatoprotective effect of a novel pigment-protein complex (phycocyanin) producing microalga: Phormidium versicolorNCC-466 using response surface methodology.

In our study, we focused on the optimization; antioxidant and hepatoprotective potentials of novel pigment-protein complex(C-PC) isolated from Phormidium versicolor against cadmium induced liver injury in rats. From analysis, the C-PC extraction parameters were optimized using the response surface methodology (RSM) for optimal recoveries of C-PC extraction. For analysis, the optimum operational conditions for maximizing phycocyanins concentration (67.45mg/g DM) were found to be water/solid 2, temperature 32.5°C and pH7.2.This pigment was identified using HPLC and FTIR analysis. In addition, the molecular masses of α and ß subunits were 17 and 19kDa. Scavenging activity of superoxide anion, hydroxyl, nitric oxide radicals and metal chelating in vitro results indicated that C-PC has an excellent capacity as antioxidant. In vivo study, C-PC significantly prevented cadmium-induced elevation of ALAT, ASAT and bilirubin levels in rats. The histopathological observations supported the results serum enzymes assays. The results of this study revealed that C-PC has significant hepatoprotective potential. C-PC (50mgkg-1 body weight) significantly enhanced the levels of antioxidant enzymes. It can be concluded that C-PC possesses prevention action against hepatotoxicity caused by cadmium.