RESUMO
A new triterpenoid bearing octacosanoate, named taraxer-3ß-yl octacosanoate (1), together with 13 known compounds (2-14), was isolated from the ethanol extract of the stems and roots of Clerodendrum philippinum var. simplex. The structure of taraxer-3ß-yl octacosanoate (1) was elucidated by extensive spectroscopic analysis. Uncinatone (8) and clerodenone A (10) exhibited inhibition of lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages with IC50 values of 12.50 and 3.18 µM, respectively.
Assuntos
Clerodendrum/química , Ácidos Graxos/química , Macrófagos/efeitos dos fármacos , Triterpenos/química , Abietanos/química , Abietanos/isolamento & purificação , Animais , Linhagem Celular , Ácidos Graxos/isolamento & purificação , Lipopolissacarídeos , Camundongos , Estrutura Molecular , Óxido Nítrico/metabolismo , Raízes de Plantas/química , Caules de Planta/química , Triterpenos/isolamento & purificaçãoRESUMO
Two new compounds, the 6-oxo oleanane-type triterpenoid uncarilic acid, and its 5,6-secotriterpenoid derivative, secouncarilic acid, were isolated from the hooks and stems of Uucaria sessilifructus together with seven known ursane-type triterpenoids. Uncarilic acid is the second 6-oxo oleanane-type triterpenoid ever reported, while secouncarilic acid is the first oleanane-type 5,6-secotriterpenoid. A plausible biosynthetic pathway from uncarilic acid to secouncarilic acid was also postulated. The inhibitory activities of all the nine compounds against LPS-induced nitric oxide production in RAW264.7 macrophages were evaluated.