Development and characterisation of monoclonal antibodies reactive with porcine CSF1R (CD115).

Macrophage colony-stimulating factor (CSF1) controls the proliferation and differentiation of cells of the mononuclear phagocyte system. CSF1, alongside a second ligand, interleukin-34 (IL-34), acts by binding to a cell surface receptor (CSF1R). We previously cloned and expressed pig CSF1 and IL-34. Here we produced a pig CSF1R-Ig+pFUSE Fc fusion protein and used it as an immunogen to produce three monoclonal antibodies (ROS8G11, ROS3A5 and ROS3B10) targeted against porcine CSF1R. Specific binding of each monoclonal antibody was confirmed by ELISA, Western blot, flow cytometry and immunocytochemistry. The antibodies did not block CSF1 signalling. The surface expression of CSF1R in pig peripheral blood was restricted to CD14-positive monocytes and was also detected on lung macrophages. These antibodies provided an opportunity to investigate the increase of available CSF1R during pig BMDM differentiation. The new monoclonal antibodies provide useful reagents to support the study of monocyte and macrophage biology in the pig.

Efectos sobre el metabolismo anaeróbico láctico del aporte de Phlebodium decumanun y de un programa de acondicionamiento físico-salud en profesores de esquí / Effects of Ph D and/or a physical/health conditioning programme on lactic anaerobic metabolism in ski teachers

Minimizar alguno de los efectos nocivos derivados del ejercicio se plantea como objetivo principal de este estudio, donde a una muestra poblacional de profesores de esquí alpino, que no hacen más ejercicio programado que su práctica laboral, se les somete a tres tipos de tratamiento, por la manipulación de dos variables independientes: programa de acondicionamiento físico–salud, aporte de una sustancia natural considerada inmunomoduladora (Phlebodium decumanum), o ambos a la vez, durante 4 semanas consecutivas. En la investigación se estudian las respuestas a ejercicio físico intenso que puede conllevar fatiga, en acciones semejantes al gesto técnico básico del esquí alpino, antes y después del tratamiento, con un diseño factorial que permitirá analizar el efecto de cada variable por sí sola o el efecto combinado. Para ello, se definen como variables dependientes: ión amonio, lactato y test de 45 segundos del Test de Bosco. Tras realizar el análisis estadístico se observan diferencias significativas en todas las variables excepto para el amonio, pudiendo observar que los mejores resultados se obtienen en el grupo donde se asociaban ambas variables. Por ello, podemos concluir de manera general que los resultados del estudio son esperanzadores para evitar riesgos potenciales de alta intensidad

Minimizing some of the harmful slide effects of physical activity is the main aim of this research. Alpine skiing monitors who did not practise more planned exercise but their jobs. Underwent three types of treatment, manipulating two independent variables: a programme of physical-health conditioning and a supplementation with a natural substance which is considered as immunomodulator (Phlebodium decumanum), or both at the same time, for consecutive four weeks. During the research the responses to the intense physical exercise which can cause fatigue because of actions that are similar to the basic technical gesture of the alpin skiing, before and after the treatment, with a factorial design, which will let it analyse the effects of each variable alone or together. As dependent variables ammonio, lactate and Bosco’s test of 45 seconds were considered. After carring out statictical analysis significant differences in all variables except for AMONIO were found. The best results were obtained in the group where both variables were combined. We can conclude saying that the results of the research give us hopes for avoiding potencial risks of high intensity exercise