Assuntos
Isquemia Miocárdica/sangue , Isquemia Miocárdica/cirurgia , Fator de Crescimento Transformador beta/sangue , Fator de Necrose Tumoral alfa/sangue , Biomarcadores , Ponte de Artéria Coronária , Progressão da Doença , Eletrocardiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Monitorização Fisiológica , Isquemia Miocárdica/diagnóstico , Isquemia Miocárdica/fisiopatologia , Período Perioperatório , Fatores de RiscoRESUMO
The effects of tetrapeptide pancragen (Lys-Glu-Asp-Trp-NH2) on blood glucose level and permeability and adhesion of mesenteric capillaries were studied in Wistar rats with experimental streptozotocin-induced diabetes mellitus. Oral pancragen produced a pronounced hypoglycemic effect during treatment. Intramuscular pancragen normalized the adhesion of mesenteric capillary endothelium, but did not modify capillary permeability. The results indicate homeostatic and endothelioprotective effects of pancragen during the early period of diabetes mellitus.
Assuntos
Glicemia/análise , Permeabilidade Capilar/efeitos dos fármacos , Diabetes Mellitus Experimental/tratamento farmacológico , Oligopeptídeos/farmacologia , Administração Oral , Animais , Adesão Celular/efeitos dos fármacos , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/induzido quimicamente , Injeções Intramusculares , Masculino , Mesentério/irrigação sanguínea , Oligopeptídeos/administração & dosagem , Ratos , Ratos Wistar , EstreptozocinaRESUMO
We studied the effect of Vilon in rats 2, 4, and 6 months after the onset of chronic renal failure. Subcutaneous injection of Vilon significantly decreased serum concentration of transforming growth factor-beta(1) and permeability of mesenteric microvessels in rats 2 months after the onset of chronic renal failure. Our results indicate that the preparation produces a potent homeostatic effect in the early period of chronic renal failure.
Assuntos
Permeabilidade Capilar/efeitos dos fármacos , Dipeptídeos/farmacologia , Endotélio Vascular/efeitos dos fármacos , Falência Renal Crônica/sangue , Fator de Crescimento Transformador beta/sangue , Animais , Capilares/citologia , Dipeptídeos/uso terapêutico , Falência Renal Crônica/tratamento farmacológico , Falência Renal Crônica/metabolismo , Masculino , Artéria Mesentérica Inferior/citologia , Artéria Mesentérica Inferior/efeitos dos fármacos , RatosRESUMO
OBJECTIVES: To characterize dynamics of changes of serum levels of TGF-beta1 and TNF-alpha in rats with cardiac fibrosis (CF) occurring during chronic renal failure (CRF), and to reveal the character pf correlations of these factors with amounts of cardiac collagen. DESIGN AND METHODS: CRF was induced by unilateral nephrectomy and by electrocoagulation of 25% of the cortex of remnant kidney. Post-operative checkpoints were 2, 4, and 6 months. Serum TGF-beta1 and TNF-alpha levels were measured by ELISA. RESULTS: CF became pronounced only at 6 months of CRF, while serum TGF-beta1 concentrations reached maximum at 4 months, i.e., at the checkpoint preceding the development of CF. Multiple regression showed cardiac collagen to correlate with both serum TGF-beta1 levels and time from the onset of CRF. Sensitivity and specificity of TGF-beta1 as serum marker of CF were 86% and 75%.
Assuntos
Falência Renal Crônica/sangue , Falência Renal Crônica/patologia , Miocárdio/patologia , Fator de Crescimento Transformador beta/sangue , Fator de Necrose Tumoral alfa/análise , Animais , Biomarcadores/sangue , Modelos Animais de Doenças , Fibrose/sangue , Masculino , Ratos , Sensibilidade e Especificidade , Fatores de Tempo , Fator de Crescimento Transformador beta1RESUMO
Mast cells, producing different cytokines, chemokines, proteases, vasoactive substances and other mediators, are multifunction cells which play an important role in physiological reactions, as well as in pathological situations. In the human heart mast cells have been identified at the site of sarcolemma, in perivascular tissue, tunica adventitia of vessels, and also in coronary atheroma. Heart mast cells have a number of immunological and functional features that make them distinct from other mast cells, the features are believed to be due to microenvironmental influences on phenotypic and cytochemical characteristics. Mast cells play complex and considerable role in development of different pathological processes in the heart. Surface receptors to lgE (Fc epsilon RI) and anaphylotoxine G5a determine their participation in development of systemic and cardiac anaphylactic reactions. Direct activation of heart mast cells by intravenously injected substances used in therapy and diagnostics can also result in development of anaphylactoid reactions. It was shown that quantity and density of mast cells is much higher in patients with atherosclerosis, myocarditis, ischemic and dilated cardiomyopathy, than in humans without any cardiac pathology. Thus, the data allow to suggest that heart mast cells play an important role in regulation of a functional state of the myocardium both under normal and pathological conditions.
Assuntos
Cardiomiopatias/patologia , Mastócitos/patologia , Mastócitos/fisiologia , Isquemia Miocárdica/patologia , Miocárdio/citologia , Animais , Cardiomiopatias/enzimologia , Cardiomiopatias/imunologia , Humanos , Mastócitos/enzimologia , Mastócitos/imunologia , Isquemia Miocárdica/enzimologia , Isquemia Miocárdica/imunologiaRESUMO
Changes of the rat skin and mesenteric microvascular permeability for Na-fluorescine were studied during adaptation to intermittent hypobaric hypoxia. The data obtained revealed that the microvascular permeability increased on the 10 th day after the hypoxia and decreased within 25 days of hte adaptation to the hypoxia in both areas under study.
Assuntos
Adaptação Fisiológica , Permeabilidade Capilar/fisiologia , Fluoresceínas/farmacocinética , Hipóxia/fisiopatologia , Mesentério/irrigação sanguínea , Pele/irrigação sanguínea , Adaptação Fisiológica/efeitos dos fármacos , Animais , Anticoagulantes/farmacologia , Câmaras de Exposição Atmosférica , Permeabilidade Capilar/efeitos dos fármacos , Feminino , Fluoresceína , Heparina/farmacologia , Mesentério/efeitos dos fármacos , Microcirculação/efeitos dos fármacos , Microcirculação/fisiologia , Microscopia de Fluorescência/métodos , Ratos , Pele/efeitos dos fármacos , Fatores de TempoAssuntos
Pressão do Ar , Permeabilidade Capilar/efeitos dos fármacos , Heparina de Baixo Peso Molecular/farmacologia , Heparina/farmacologia , Hipóxia/fisiopatologia , Pele/irrigação sanguínea , Animais , Câmaras de Exposição Atmosférica , Feminino , Heparina/farmacocinética , Heparina de Baixo Peso Molecular/farmacocinética , Ratos , Pele/efeitos dos fármacos , Fatores de TempoRESUMO
The functional status of a population of mast cells taken from the subcutaneous connective tissue of rats with lymphosarcoma of Pliss and those suffering from aseptic inflammation was evaluated. The experiment established such manifestations of remote influence on the mast cell population, at early stages of tumorigenesis, as faster rates of tumor growth and mast cells maturation and enhanced degranulation. Initial signs of functional exhaustion of the mast cells population was seen as a paraneoplastic syndrome.
Assuntos
Tecido Adiposo/fisiopatologia , Linfoma não Hodgkin/fisiopatologia , Mastócitos/fisiologia , Animais , Celulite (Flegmão)/fisiopatologia , Síndromes Paraneoplásicas/fisiopatologia , RatosRESUMO
The effects of heparin on the permeability of skin vessels were investigated in intact rats with lymphosarcoma of Pliss. It has been ascertained that heparin treatment diminishes skin vessels permeability in both intact rats and those with blastoma. More pronounced effect was observed in the experiments involving chronic heparinization of animals, the permeability of vessels in tumor-bearing animals being lower than in intact ones.
Assuntos
Vasos Sanguíneos/efeitos dos fármacos , Heparina/farmacologia , Linfoma não Hodgkin/fisiopatologia , Permeabilidade/efeitos dos fármacos , Pele/irrigação sanguínea , Animais , Vasos Sanguíneos/fisiopatologia , Masculino , RatosRESUMO
Mast cells of the rat subcutaneous connective tissue were studied in experimental hypertension. An increase was discovered in the degranulation of the cells in rats with spontaneous and adrenal-regenerative hypertension. These animals demonstrated a rise in the number of immature cells with a relatively low content of serotonin. The changes described are believed to be linked with arterial pressure elevation.