RESUMO
BACKGROUND: The aim of this study was to analyze the association of eNOS polymorphisms with risk of Legg-Calve-Perthes Disease (LCPD). METHODS: The study comprised of 45 LCPD patients and 55 controls. The eNOS polymorphisms were genotyped with PCR and by PCR-RFLP. RESULTS: The eNOS 894Gâ¯>â¯T and -786Tâ¯>â¯C polymorphisms were significantly associated with an increased risk of LCPD. However, there was no significant association between eNOS 27-bp VNTR polymorphism and LCPD risk. CONCLUSION: Our results suggest that the eNOS 894Gâ¯>â¯T and -786Tâ¯>â¯C polymorphisms may be a risk factor for LCPD in Iranian children, but not 27-bp VNTR polymorphism.
RESUMO
OBJECTIVE: The aim of this study was to assess the association of MTHFR and TNF-α genes polymorphisms with Legg-Calvé-Perthes' disease (LCPD) risk in the Iranian children. METHODS: A total of 45 children with LCPD and 55 healthy controls were recruited to the study. Genotyping was performed via the RFLP-PCR method and genetic risk was calculated by odds ratio (OR) with its corresponding 95% confidence interval (CI). RESULTS & CONCLUSION: Our case-control study failed to determine any association of MTHFR (677Câ¯>â¯T and 1298Aâ¯>â¯C) and TNF-α (-308Gâ¯>â¯A and -238Gâ¯>â¯A) polymorphisms with LCPD risk. More studies with larger sample size are warranted to validate our findings.