RESUMO
The majority of fractures of the anterior pelvic ring is treated non-operatively. However, a number of patients do not get pain free and cannot be mobilized. Since the supra-acetabular external fixator is associated with significant complications we developed an alternative technique based on recent anatomical studies. This article is a clinical feasibility study to evaluate a novel stabilization technique for fractures of the anterior pelvic ring in the elderly patient. This technique obtains rapid pain reduction and early ambulation in this group of patients.
Assuntos
Placas Ósseas , Fixadores Externos , Procedimentos Ortopédicos/métodos , Fraturas por Osteoporose/cirurgia , Ossos Pélvicos/lesões , Acetábulo , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Ossos Pélvicos/cirurgiaAssuntos
Miosinas/metabolismo , Plasmodium berghei/crescimento & desenvolvimento , Plasmodium berghei/metabolismo , Sequência de Aminoácidos , Animais , Estágios do Ciclo de Vida , Dados de Sequência Molecular , Miosinas/química , Miosinas/genética , Plasmodium berghei/genética , Plasmodium berghei/patogenicidade , Plasmodium falciparum/genética , Plasmodium falciparum/metabolismo , Análise de Sequência de DNARESUMO
1. Adenosine, adenosine triphosphate (ATP) and some stable analogues of adenosine inhibited field stimulation-induced contractions of the uterus from rats treated with oestradiol cypionate (20 micrograms/kg, s.c.) 1 day previously. Adenosine was twice as potent as ATP; both were potentiated by dipyridamole (10 mumol/L). 2. The order of agonist potency of adenosine and its analogues was: 5'-N-ethylcarboxamidoadenosine (NECA) > N6-cyclohexyladenosine > or = R-phenylisopropyladenosine = S-phenylisopropyladenosine = 2-chloroadenosine > or = adenosine > or = ATP > > 2-p-(2-carboxyethyl) phenethylamino-5'-N-ethylcarboxamidoadenosine. This order suggests the presence of P1 purinoceptors of the A2B subtype. 3. Responses to agonists were antagonized to differing extents by the P1 purinoceptor antagonist 8-phenyltheophylline (10 mumol/L). 4. In uterine preparations from rats pretreated for 2 days with oestrogen (20 micrograms/kg, s.c.) and for 1 day with progesterone (3 mg/animal, s.c.), the inhibitory potencies of adenosine and NECA were reduced, indicating hormonal regulation of uterine responsiveness to P1 purinoceptor agonists. 5. Stable analogues of ATP caused contractions of unstimulated myometrial preparations from oestrogen-treated animals, indicating activation of a P2 purinoceptor, possibly of the P2X subtype, because of the relative order of potency was alpha, beta-methylene ATP > beta, gamma-methylene ATP = ATP = 2-methylthio ATP.
Assuntos
Miométrio/metabolismo , Receptores Purinérgicos P1/metabolismo , Receptores Purinérgicos P2/metabolismo , Nucleotídeos de Adenina/metabolismo , Animais , Estimulação Elétrica , Estrogênios/farmacologia , Feminino , Técnicas In Vitro , Progesterona/farmacologia , Agonistas do Receptor Purinérgico P1 , Agonistas do Receptor Purinérgico P2 , RatosRESUMO
The aim of this study was to characterise the muscarinic receptor present in the uterus of the virgin rat. Homogenate binding studies were undertaken using [3H]quinuclidinyl benzilate as the radioligand and atropine (10 microM) to determine non-specific binding. [3H]Quinuclidinyl benzilate binding was saturable with a Kd of 63 pM and a Bmax of 3 fmol/mg protein. The pKi values obtained using antagonists with high affinity for differing muscarinic receptor subtypes were pirenzepine, 6.2; hexahydrosiladifenidol, 6.9; AF-DX 116 (11-[[2-[(diethylamino)methyl]-1-piperidinyl]acetyl]5,11-dihydro-6H - pyrido[2,3-b][1,4]benzodiazepine-6-one), 7.0; and himbacine, 7.8. These findings suggest that muscarinic M2 receptors are present in rat uterus.